id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-314954-otc0pc09	Liu, Xiaoli	Identification of a novel linear B-cell epitope in the UL26 and UL26.5 proteins of Duck Enteritis Virus	2010-09-13		.txt	text/plain	4270	224	60	In this study, the C-terminus of DEV UL26 protein (designated UL26c), which contains the whole of UL26.5, was expressed, and the recombinant UL26c protein was used to immunize BALB/c mice to generate monoclonal antibodies (mAb). Both western blotting and ELISA showed that 1C8 could react specifically with both chicken embryonic fibroblasts (CEF) infected with DEV ( Figure 1B and Figure 1C ) and the recombinant protein UL26c ( Figure 1D and Figure 1C ). The results of both western blotting and ELISA showed that this peptide defined by mAb 1C8 could react with murine anti-DEV antibody ( Figure 4 ), which demonstrated that the epitope had good reactivity. A series of 17 fragments that spanned the UL26c protein were expressed with a GST tag in this study, and used to screen for the minimal epitope recognized by mAb 1C8 using western blotting and ELISA. The specificity and reactivity of the mAb 1C8 were also determined by western blotting using recombinant UL26c protein and CEF infected by DEV, respectively.	./cache/cord-314954-otc0pc09.txt	./txt/cord-314954-otc0pc09.txt