id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-290798-5ca2e6wm	Malhotra, Bharti	Evaluation of custom multiplex real - time RT - PCR in comparison to fast - track diagnostics respiratory 21 pathogens kit for detection of multiple respiratory viruses	2016-06-06		.txt	text/plain	3019	155	56	title: Evaluation of custom multiplex real time RT PCR in comparison to fast track diagnostics respiratory 21 pathogens kit for detection of multiple respiratory viruses Therefore the aim of the present study was to develop a cost effective multiplex real time RT-PCR for the detection of 18 respiratory viruses and compare it with an in-vitro diagnostics approved Fast Track Diagnostic Respiratory Pathogens 21 Kit (FTD). METHODS: Nasopharyngeal aspirates and throat swabs were collected and processed for extraction of nucleic acid using an automated extraction system and multiplex real time RT-PCR was performed using the FTD kit and a custom assay on 356 samples. The present study compares custom real-time multiplex PCR primers and probes for the simultaneous detection of 18 respiratory viruses with an in-vitro diagnostics (IVD) approved fast track diagnostics (FTD) kit.	./cache/cord-290798-5ca2e6wm.txt	./txt/cord-290798-5ca2e6wm.txt