id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-285253-ik5w4t5e	Ali, S Asad	Real-world comparison of two molecular methods for detection of respiratory viruses	2011-06-29		.txt	text/plain	3414	177	49	METHODS: We tested nasal and throat swab specimens obtained from 225 infants with respiratory illness for 11 common respiratory viruses using both a multiplex assay (Respiratory MultiCode-PLx Assay [RMA]) and individual real-time RT-PCR (RT-rtPCR). The RMA assay detected significantly more human metapneumovirus (HMPV) and respiratory syncytial virus (RSV), while RT-rtPCR detected significantly more influenza A. Restricting the analysis to the 11 viruses tested with both methods, at least one virus was detected in 174 (78.3%) samples by RMA and in 163 (73.4%) samples by RT-rtPCR. In the second round using redesigned RT-rtPCR HMPV primers and probes, HMPV was detected in 7 samples, 5 of which were concordant with the results of the first RMA assay. Comparison of the Eragen Multi-Code Respiratory Virus Panel with conventional viral testing and real-time multiplex PCR assays for detection of respiratory viruses	./cache/cord-285253-ik5w4t5e.txt	./txt/cord-285253-ik5w4t5e.txt