id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-283689-dzin12qb	Zhang, Wei	Involvement of PRRSV NSP3 and NSP5 in the autophagy process	2019-01-28		.txt	text/plain	5098	320	51	RESULTS: Autophagy was activated by porcine reproductive and respiratory syndrome virus (PRRSV) NSP3, NSP5 and NSP9, which are two transmembrane proteins and an RNA-dependent RNA polymerase, respectively. Based on the data presented in Fig. 2c , immunoblotting and immunofluorescence assay showed that the expression of the p62 protein was increased, indicating that NSP3 and NSP5 of PRRSV induced the formation of autophagosomes. As shown in Fig. 3a , ATG5 puncta were arranged in reticular structures and colocalized with calnexin in the Fig. 1 The distribution of autophagy proteins in PRRSV-infected Marc-145 cells. As shown in the present study, the induction of autophagy by PRRSV NSP3 and NSP5 contributed to the formation of autophagosomes derived from the ER, and the mature autophagosomes were not degraded by fusion with lysosomes.	./cache/cord-283689-dzin12qb.txt	./txt/cord-283689-dzin12qb.txt