id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-269277-x5c5ogo7	Li, Jingjing	Key elements of the human bocavirus type 1 (HBoV1) promoter and its trans-activation by NS1 protein	2013-10-27		.txt	text/plain	4114	202	54	The pGL3-Bov-EGFP vector containing nt 1-252 was transfected into 293 T, A549, HeLa and WI-38 cell lines to assay the activity of the promoter of HBoV1 in these mammalian cells. Here, the activity of the HBoV1 promoter in cells co-transfected with P1 and pGL3-pCMV-NS1mut vector (no NS1 protein expression) was used as a control. In our recent study, NS1 transcripts from the left-hand of the viral genome were detected in pWHL-1 transfected 293 T cells and other mammalian cells including HeLa, A549, WI-38 cell lines (data not shown), suggesting that this promoter is unique and active in most mammalian cells. For detection of HBoV1 promoter activity, mammalian cell lines including 293 T, A549, HeLa and WI-38 were transfected with the pGL3-Bov-EGFP construct and the pEGFP-N1 control vector in 24-well plates. Key elements of the human bocavirus type 1 (HBoV1) promoter and its trans-activation by NS1 protein	./cache/cord-269277-x5c5ogo7.txt	./txt/cord-269277-x5c5ogo7.txt