id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-261579-f0prnpsu	Eichler, Robert	The role of single N-glycans in proteolytic processing and cell surface transport of the Lassa virus glycoprotein GP-C	2006-05-31		.txt	text/plain	2557	138	51	title: The role of single N-glycans in proteolytic processing and cell surface transport of the Lassa virus glycoprotein GP-C In this report, we investigated the effect of each N-glycan on proteolytic cleavage and cell surface transport by disrupting the consensus sequences of eleven potential N-glycan attachment sites individually. To address this question, we investigated in the present report each potential N-glycosylation site of the Lassa virus glycoprotein concerning N-glycan maturation, cleavage of GP-C and glycoprotein transport to the cell surface. In order to investigate the importance of the individual N-glycosylation sites of Lassa virus GP-C for intracellular trafficking we analysed cell surface expression of the mutated glycoproteins using a biotinylation approach. Taken together, our study suggest that individual N-linked oligosaccharides of the Lassa virus glycoprotein differ greatly in terms of their importance for correct protein folding which seems to be important for activation cleavage by SKI-1/S1P.	./cache/cord-261579-f0prnpsu.txt	./txt/cord-261579-f0prnpsu.txt