id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-000988-79fp75u3	Al-Siyabi, Turkiya	A cost effective real-time PCR for the detection of adenovirus from viral swabs	2013-06-07		.txt	text/plain	6247	327	43	Twentyseven virus culture-positive specimens and 169 virus culture-negative specimens were randomly selected and tested for the presence of HAdV using a well established in-house real-time PCR assay [18] following recovery of viral DNA was recovered by homogenization with heat treatment or automated nucleic acid extraction. This internally controlled quantitative real-time PCR assay targets the hexon gene of adenovirus, and is validated for detection Table 1 Nucleotide sequences of primers and probes used in this study The analytical sensitivity (or limit of detection, LoD) of the homogenization with heat treatment or nucleic acid extraction, in combination with the real-time PCR, was determined using 10-fold serial dilutions (in UTM) of a cultured HAdV-C type 6. Virus stock dilutions were quantified using commercial real-time PCR assay, and the LoD for homogenization or nucleic acid extraction-based protocols were shown to be approximately equivalent (Figure 2) .	./cache/cord-000988-79fp75u3.txt	./txt/cord-000988-79fp75u3.txt