id author title date pages extension mime words sentences flesch summary cache txt cord-334968-gonx5taq Pignatelli, Jaime Lineage specific antigenic differences in porcine torovirus hemagglutinin-esterase (PToV-HE) protein 2013-12-23 .txt text/plain 6772 291 50 In the first case, the two PToV-HE lineages were detected even within the same animal at two sequential sampling time points, indicating that both PToV strains carrying different HE proteins coexisted on the same farm infecting the same piglets, and suggesting that the immune response generated against one PToV strain did not protect the animals against the infection by the other strain. Hence, in order to examine the potential existence of antigenic differences between the two lineages of PToV-HE proteins, piglet serum samples were analyzed with an HI assay to detect the presence of specific antibodies against the receptor binding domain that prevents the RBC hemagglutination. In addition, to study the antibody response against the whole protein by a different approach, soluble c-myc tagged HE52.7 and HE52.11 proteins were generated by rVV methodology to obtain highly purified coating antigens that were used in ELISA to test the same field serum samples. ./cache/cord-334968-gonx5taq.txt ./txt/cord-334968-gonx5taq.txt