id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-264888-h560slug	Goossens, Evy	The C-terminal domain of Clostridium perfringens alpha toxin as a vaccine candidate against bovine necrohemorrhagic enteritis	2016-04-27		.txt	text/plain	5355	283	44	title: The C-terminal domain of Clostridium perfringens alpha toxin as a vaccine candidate against bovine necrohemorrhagic enteritis Using an intestinal loop model in calves, we investigated the protection afforded by antisera raised against native alpha toxin or its non-toxic C-terminal fragment against C. The aim of this study was to evaluate whether the nontoxic C-terminal fragment of alpha toxin could be a candidate for effective vaccination of calves against bovine necrohemorrhagic enteritis. Incubation of cell-free supernatants of the wild-type strain JIR325 (concentrated tenfold using Vivaspin, Sartorius Stedim Biotech GmbH, Göttingen, Germany) on sheep blood agar at 37 °C overnight results in an inner, complete zone of hemolysis caused by perfringolysin O and a less complete outer zone caused by alpha toxin. perfringens strain by sera from calves immunized with the native alpha toxin (rCpa) or the non-toxic C-terminal fragment of the alpha toxin (Cpa 247-370 ). perfringens in the intestinal loop model was significantly reduced only by co-administration of antisera from animals vaccinated with the native alpha toxin.	./cache/cord-264888-h560slug.txt	./txt/cord-264888-h560slug.txt