id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-259296-qsaewje2	Wang, Pengcheng	Tomatidine inhibits porcine epidemic diarrhea virus replication by targeting 3CL protease	2020-11-11		.txt	text/plain	8180	490	54	We screened an FDA-approved library of 911 natural products and found that tomatidine, a steroidal alkaloid extracted from the skin and leaves of tomatoes, demonstrates significant inhibition of PEDV replication in Vero and IPEC-J2 cells in vitro. After transfer, the membrane was incubated in blocking buffer (5% non-fat milk in PBST w/v) for 2 h at room temperature, washed three times with PBST, then probed with the following antibodies: anti-PEDV N-protein ( The detection of mRNA levels of TGEV, PRRSV, and SVA was performed as described previously [13, 19, 20] . When Vero cells were treated with increasing concentrations of tomatidine, the cleaved fragments clearly decreased in a dose-dependent manner ( Figure 6B ), indicating that tomatidine inhibited the activity of PEDV 3CL protease.	./cache/cord-259296-qsaewje2.txt	./txt/cord-259296-qsaewje2.txt