key: cord-278641-8lh5y7j0
authors: Chen, Jianing; Cui, Yaru; Wang, Zemei; Liu, Guangliang
title: Identification and characterization of PEDV infection in rat crypt epithelial cells
date: 2020-09-17
journal: Vet Microbiol
DOI: 10.1016/j.vetmic.2020.108848
sha: 
doc_id: 278641
cord_uid: 8lh5y7j0

Porcine epidemic diarrhea (PED) is a devastating enteric disease to the world's swine production. Porcine epidemic diarrhea virus (PEDV), as the PED causative agent, has been commonly propagated and investigated in Vero cells, as well as in IPEC-J2, a porcine epithelial cell-jejunum 2. However, Vero cells, which are defective in interferon production, cannot represent the host response in enteric cells while PEDV replicates poorly in IPEC-J2 cells. In this study, we observed that rat crypt epithelial cells (IEC-6) were highly susceptible to different subtypes of PEDV. The replication kinetics of PEDV in IEC-6 cells is similar to that in Vero cells, but it is much higher than in IPEC-J2 cells. Besides that, PEDV infection in IEC-6 cells can induce the production of inflammatory cytokines and interferon, especially the type III IFNs. Collectively, our findings suggest that IEC-6 is an ideal cell line for PEDV replication and immune response studies.

The swine enteric coronaviruses, a family of the most significant problematic pathogens to the world's swine industry, include porcine epidemic diarrhea virus (PEDV), porcine transmissible gastroenteritis virus (TGEV), porcine deltacoronavirus (PDCoV), and swine acute diarrhea syndrome coronavirus (SADS-CoV) (Qiuhong et al., 2019) .

Among these four viruses, PEDV has been recognized as the most devastating pathogen, causing up to 100% mortality in piglets younger than one week old. Since its first discovery in the 1970s, PEDV has then subsequently identified nearly worldwide (Wood, 1977) .

Later in the 2010s, variant PEDV strains were emerged and killed hundreds of millions of suckling piglets (Jung and Saif, 2015; Wang et al., 2016) .

PEDV is an enveloped, single-stranded, positive-sense RNA virus, which targets the small intestine, especially the ileum (Lee, 2015) . PEDV infection in enterocytes can exhibit acute necrosis, leading to marked villous atrophy in the small intestine (Ducatelle et al., 1982) . Consequently, malabsorption, caused by the massive loss of absorptive enterocytes, contributes to diarrhea and even death of newborn piglets. The cell line, which is permissive for PEDV replication and possesses the intestine function and responses, may facilitate the studies about PEDV. Vero cells are commonly used for PEDV propagation.

However, this cell line is characterized by lacking the production of interferon (Desmyter et al., 1968) . Consequently, it is valuable in vaccine production but defective in immune studies. IPEC-J2 is a non-transformed porcine columnar epithelial cell line and used in the investigations of PEDV Lin et al., 2017) . However, it is semi-permissive to PEDV infection and defective in the production of several cytokines (Brosnahan and J o u r n a l P r e -p r o o f Brown, 2012) . These drawbacks of the two cell lines restrict further understandings of PEDV. Therefore, it is of great importance to screen proper cell lines for PEDV investigations.

In this study, we identified rat crypt epithelial cells (IEC-6) was highly susceptible to a different subtype of PEDV, which could be an ideal cell model for PEDV studies.

The rat small intestine epithelium IEC-6 cells were purchased from American Type Culture Collection (ATCC) and cultured in Dulbecco's modified Eagle's medium (DMEM) (Sigma, Germany) supplemented with 0.1 Unit/ml bovine insulin and 10% fetal bovine serum (Gemini, USA). African green monkey epithelial Vero cells were cultured in DMEM medium supplemented with 10% fetal bovine serum. The intestinal porcine epithelial cell line J2 (IPEC-J2) cells were cultured in DMEM/F12 (Sigma, Germany) supplemented with 10% fetal bovine serum, 5 μg/ml insulin/selenium/transferrin (Life, USA) and 5 ng/ml epidermal growth factors (EGF) (Life, USA). The PEDV LJX01/GS/2014 strain was isolated and conserved in our laboratory. The PEDV CV777 strain were isolated from attenuated vaccine and maintained in our laboratory.

IEC-6 cells were seeded in 48-well plates. When grown to 80% confluency, cells were infected with PEDV at a MOI=0.01. After 48 hours, the cells were fixed with 4% J o u r n a l P r e -p r o o f paraformaldehyde for 30 min and then permeabilized with 0.5% Triton X-100 for 15 min at room temperature. After blocking with 5% skim milk for 1 h, cells were incubated with mouse anti-PEDV-nucleocapsid monoclonal antibody (1:5000 dilution) for 1 h, followed by incubating with the Alexa Fluor 488 goat anti-mouse IgG antibody (1:1000 dilution) (Thermo, USA) for 1 h. The cell nuclei were stained by DAPI (Beyotime, China). The cells were then examined under a fluorescence microscope (TE2000U; Nikon) with a video documentation system.

To assess the host response against PEDV, the three cell lines were infected with PEDV at a MOI=0.01. The supernatant was collected at 12, 24, 36, 48, 60 and 72 hours postinfection and used for viral titration. At 24 hours post-infection, the cells were also lysed with TRIzol (Takara, Japan) for RNA extraction.

Confluent monolayers of IEC-6 cells in 6-well plates were inoculated with PEDV and incubated for 1 h at room temperature on rocker. After three washes, the cells were overlaid with 0.75% low melting point agarose (Sigma, Germany) in DMEM containing 5% FBS and then incubated at 37℃ for around 72 h. To visualize plaques, cells were stained with 1% crystal violet in ethanol.

Vero cells were seeded into 96-well plates and cultured for 80-90% confluence. The cell monolayers were washed three times with PBS. Serial 10-fold dilutions of the collected J o u r n a l P r e -p r o o f supernatant were made, and 100 l of each dilution were inoculated to five wells of the 96well plate. The plates were incubated at 37 • C and supplied with 5% CO2. And then, the cultures were checked under a light microscope for cytopathology every day for 3 days.

The dilution of the supernatant was titrated by TCID50 assay and calculated based on the Reed-Muench method.

For the detection of host gene expression level, the real-time qPCR was performed with unique aptamer qPCR SYBR green master mix (Novogen, China) in the Bio-Rad CFX96 system. The reactions were incubated at 94°C for 30 s, followed by 45 cycles at 94°C for 5 s and 60°C for 30 s. All reactions were run in triplicate using the primer sets listed in Table 1 . The 2 -∆∆Ct method was employed to measure the expression level of target genes.

The Student's t-test was used for the examination of statistical significance between matched groups. An unadjusted P value of less than 0.05 was considered significant; a P value of less than 0.01 was considered highly significant.

IEC-6 cell line, originally derived from the rat small intestinal crypt, was commonly used in the nutritional investigations (Quaroni et al., 1979) . To investigate if it is susceptible to PEDV, the IEC-6 cells were infected with two different subtypes of PEDV at a MOI=0.01 and observed for cytopathic effect (CPE) under a microscope. The PEDV J o u r n a l P r e -p r o o f vaccine strain CV777 belongs to subtype G1, while the field strain LJX isolated by our group belongs to subtype G2 (Guo et al., 2018) . As shown in Fig. 1A , typical CPE became visible at 24 hours post-infection. The morphology of PEDV-infected cells became enlarged fusiform at early stage. Lately, they were wrinkled and detached. However, the mock-infected cells remained normal during the whole process. IFA was then performed to confirm its infection by using a mAb against PEDV nucleocapsid protein (Yang et al., 2019) . The results showed that strong PEDV-N positive signals were observed in IEC-6 cells infected with either LJX strain or CV777 strain (Fig. 1B) , indicating IEC-6 cells were successfully infected by PEDV. Then the total RNA was extracted and analyzed by RT-PCR. The results demonstrated that the PEDV-specific bands were amplified from virusinoculated cells (Fig. 1C) . Finally, the western blotting analysis showed that the PEDV nucleocapsid protein was detected in cells infected with PEDV (Fig. 1D) . We also attempted to identify whether the IEC-6 were susceptible to TGEV but no positive signal were obtained (data not shown). Taken together, the above data illustrated that IEC-6 cells were susceptible to PEDV but not TGEV.

Vero and IPEC-J2 cell lines were commonly used for PEDV investigation. To test and compare the replicative capacity of PEDV in different cell lines, the PEDV replication kinetics were measured in these three cell lines. These cells were seeded in the 24-well plate and inoculated with PEDV LJX strain or CV777 strain at a MOI=0.01. The supernatant was collected every 12 hours and used for viral titration. The results showed that the PEDV LJX strain replicated poorly in IPEC-J2 cells with viral titers lower than 10 4 J o u r n a l P r e -p r o o f TCID50/ml. PEDV LJX strain exhibited a robust and similar growth curve in Vero and IEC-6 cells, showing higher than 10 7 TCID50/ml viral titers. PEDV CV777 strain reached its most elevated viral titers in both Vero and IEC-6 cells but poorly replicated in IPEC-J2 cells (Fig. 1E) . The plaque assay is usually employed for virus purification and titration. It is of great importance to virus related studies. We also tested whether IEC-6 could be used to conduct plaque assay. The data showed that the infection of both LJX and CV777 strains resulted to the formation of plaque (Fig. 1F) . These results further showed that IEC-6 cells supported PEDV infection and replication.

PEDV infection in the porcine small intestine is characterized by inflammatory cytokines and interferon (Jung and Saif, 2015) . The type III interferon is considered to play a critical role in enteric immunity. Therefore, we examined whether PEDV infection could induce the typical enteric immunity in IEC-6 cells compared to its infection in IPEC-J2 and Vero cells. PEDV LJX was a field strain and was used to infect the three cell lines.

After 24 hours post-infection, the RNA was extracted for RT-qPCR analysis. The results showed that both type I and type III interferon responses were significantly activated in PEDV infected IEC-6 and IPEC-J2 cells while the type I interferon were absent in Vero cells ( Fig. 2A, 2B, 2D, 2E ). Due to the lack of IFN-λ3 gene of green monkey in the NCBI or ENSEMBL database, we failed to detect its gene expression level. Although the IFN-λ3 gene of green monkey was strongly upregulated in PEDV infected Vero cells, its Ct value was about 30, while the Ct value to GAPDH was about 18 in PEDV infected cells. In mock J o u r n a l P r e -p r o o f cells, the Ct values of about 39 for IFN-λ3 and 18 for GAPDH. For type II interferon, PEDV infection led to a significant upregulation in IEC-6 cells but not in IPEC-J2 and Vero cells (Fig. 2C) . PEDV infection also enhanced the expression of IL-1, IL-6, IL-8, and

TNFα in IEC-6 cells except for IL-17A (Fig. 2F, 2G, 2H, 2I, 2J ). All these results demonstrated that IEC-6 cells were able to generate a robust immune response against PEDV infection.

Although Vero and IPEC-J2 were commonly used in PEDV studies, they were defective in immune response and viral replication. There were also other cell lines identified permissive for PEDV infection recently. PEDV can also infect HEK293T, IP2-2I cells, and primary bovine mesenchymal cells (Jung et al., 2020; Wang et al., 2019; Zhang et al., 2017) . However, HEK293T is not an intestinal epithelium cell line, while IPI-2I is semi-permissive to PEDV infection. The primary bovine mesenchymal cells cannot culture for a long time. The rat small intestinal crypt epithelium cell line IEC-6 is non-transformed.

And it was often used as a necrotizing enterocolitis model system and nutritional studies (Braga-Neto et al., 2012; Ruthig and Meckling-Gill, 1999; Yan et al., 2018) . The uptake, metabolism, and transport processes are observed in IEC-6 cells (Said et al., 1997; Sanderson and He, 1994) . Therefore, IEC-6 is recognized as an ideal model for intestine relating studies. However, it is rarely used in viral studies. Only modified vaccinia virus Ankara was reported to multiply in IEC-6 cells (Okeke et al., 2006) . In this study, we tested the susceptibility of IEC-6 cells to PEDV infection and found it was highly susceptible.

The growth curve of PEDV in IEC-6 was similar to it in Vero cells with the viral titers higher than 10 7 TCID50/ml. It was also found that IEC-6 could mimic the antiviral immune response in the small intestine. PEDV infection in the small intestine is characterized by the induction of proinflammatory responses (Huan et al., 2017) . In our study, IL-1b, IL-6, IL-8, and TNFα were upregulated by PEDV infection in IEC-6 cells. Only TNFα, IL-17A and IL-8 were found increasingly expressed in IPEC-J2 cells. IL-6 was only activated by PEDV infection in IEC-6 cells. Besides that, interferon is recognized to play critical roles in the innate immune response. The type III interferon is significantly essential in maintaining intestinal homeostasis, especially to PEDV (Ingle et al., 2018; Zhang et al., 2018) . In PEDV infected IEC-6 cells, the expression of type I and type III interferon was drastically upregulated, while type II interferon was also increasingly expressed. Vero cells are defective in interferon production. The Ct values of all genes were higher than 29 while it was about 20 for GAPDH. Although IFNλ1 was highly upregulated in Vero cells, its CT value was also higher than 29. In IPEC-J2 cells, the production of IFNα, IFNλ1, and IFN-λ3 was increased but not as high as in IEC-6 cells. All these data suggested that PEDV infection in IEC-6 cells could induce strong antiviral responses.

In summary, our studies identified the rat small intestinal crypt epithelium IEC-6 cells were highly susceptible to PEDV. PEDV could efficiently replicate in IEC-6 cells as it does in Vero cells. Besides that, PEDV infection activated potent inflammatory cytokines and J o u r n a l P r e -p r o o f interferon, primarily type III interferon in IEC-6 cells, which highly simulated its infection in the small intestine. All these data above suggested that IEC-6 cells can be used as an excellent cell model for PEDV studies. 

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