id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-333366-j3yh5v1g	Zhao, Dongmin	Peptide inhibitors of tembusu virus infection derived from the envelope protein	2020-05-07		.txt	text/plain	5074	302	56	DN59, an inhibitory peptide corresponding to the stem region of dengue virus (DENV) E protein, almost completely inhibited DENV infection in LLC-MK2 cells and exhibited cross-inhibitory activity against West Nile virus (WNV) infection. The data demonstrated that TP1 inhibited TMUV infection through destroying the integrity of the viral particles, while both TP1 and TP2 exerted inhibitory effects by interfering with the binding of TMUV to cells. At 24 h post-infection, qRT-PCR, plaque assays, and western blotting were conducted to detect virus production with peptide treatment. To explore the mechanism of the inhibitory effects of TP2, a virus binding inhibition assay was performed to determine whether the peptides interfered with the binding between TMUV and cells. The direct binding of TP1 or TP2 to TMUV, but not to target cells, might explain why both peptides exhibited similar inhibitory effects in the different cell types BHK-21 cells, CEFs, DF-1 cells and DEFs. The mechanism by which flavivirus infection is inhibited by peptide inhibitors is not clearly understood.	./cache/cord-333366-j3yh5v1g.txt	./txt/cord-333366-j3yh5v1g.txt