id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-296611-ma32oz4o	Ma, Tianxin	Novel genotype of infectious bronchitis virus isolated in China	2019-01-29		.txt	text/plain	5906	287	52	Further comparative genomic analysis revealed at least two recombination sites that replaced the spike gene in a lineage 18 within genotype I (GI-18)-like virus with an as-yet-unidentified sequence, likely derived from another IBV strain, resulting a novel serotype with a lower affinity to the respiratory tract in chickens. To the best of our knowledge, this provides the first evidence for recombination leading to replacement of the complete spike gene and the emergence of a novel genotype/serotype with a lower affinity to the respiratory tract in chickens comparing to one of its parental virus ck/CH/LGX/111119. To confirm these recombination breakpoints, three phylogenetic trees were constructed on the basis of the results of similarity plot (SimPlot) analysis for the nucleotide fragments 1-20,265 (5′ untranslated region (UTR) to 3′ end of Gene 1), 20,266-24,151 (3′ end of Gene 1 to 5′ end of Gene 3), and 24,152-27,629 (5′ end of Gene 3 to 3′ UTR), from nine IBV strains, including I0636/16, five strains (LGX/ 111119, CK/CH/GD/GZ14, γCoV/ck/China/I0114/14 (I0114/14), GX-YL9, and GX-YL5) showing close relationships with I0636/16 based on the complete genomic sequence analysis, and three Mass strains (Beaudette, H120, and M41) as an outgroup.	./cache/cord-296611-ma32oz4o.txt	./txt/cord-296611-ma32oz4o.txt