id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-285335-agm4zbcx	Kennedy, Melissa	Deletions in the 7a ORF of feline coronavirus associated with an epidemic of feline infectious peritonitis	2001-08-08		.txt	text/plain	2292	120	55	A population of Persian cats experienced an epidemic of feline infectious peritonitis (FIP) over 2 years. Feline coronavirus (FCoV) genomic RNA was detected consistently in this study in biologic samples from adult cats, kittens suffering from FIP, and their siblings. Analysis of viral 7a/7b open reading frame (ORFs) were analyzed and revealed two distinct virus variants circulating in the population, one with an intact 7a ORF and one with two major deletions in the 7a ORF. Both virus variants were identified in one cat, the sire ''Dan'', as sequence analysis of clones from a single PCR from this animal revealed the presence of the 7a deletion mutant as well as the intact isolate (Dan 1 and 2 in Fig. 2 ). The sire of the majority of FIP kittens was dually infected with both virus variants as revealed by sequence analysis of cloned 7a/7b genes from this cat.	./cache/cord-285335-agm4zbcx.txt	./txt/cord-285335-agm4zbcx.txt