id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-268918-6l02una0	Kapil, Sanjay	Characterization of bovine coronavirus isolates/from eight different states in the USA	1999-06-30		.txt	text/plain	3359	175	54	Antigenic differences were observed among the isolates in a one-way hemagglutination-inhibition (HI) test using a polyclonal antiserum against the Mebus bovine coronavirus isolate. Minor antigenic and biological differences have been observed among BCV isolates from calf diarrhea and winter dysentery cases from the USA (Tsunemitsu and Saif, 1995) and Canada (Dea et al., 1995) . We compared antigenic differences on one-way hemagglutination-inhibition (HI) test, isoelectric focusing of BCV proteins, and relative susceptibility of BCV isolates to hygromycin B. We observed some differences in cytopathic effects among the calf diarrhea isolates; however, passage number, plaque purification, and the presence of trypsin and pancreatin in the cell culture medium greatly affected the cytopathology of BCV isolates. Antiserum against cell culture propagated bovine coronavirus isolates: on the basis of a one way HI test, 77 BCV isolates were inhibited from 1 : 2 up to 1 : 1024.	./cache/cord-268918-6l02una0.txt	./txt/cord-268918-6l02una0.txt