id author title date pages extension mime words sentences flesch summary cache txt cord-265765-zpf1bla8 Holland, Robert E Characterization of eae(+)Escherichia coli isolated from healthy and diarrheic calves 1999-05-14 .txt text/plain 4608 243 56 Strains of Escherichia coli from 101 healthy and 114 diarrheic calves were screened by PCR for the eae (intimin) gene and Shiga toxin genes (stx). Moreover, Shiga toxin antigens were detected significantly more (p = 0.001) often among the eae/stx(+) strains from healthy calves when compared to eae/stx(+) strains from diarrheic calves. No significant difference (p ≥ 0.05) was detected among the eae(+) and eae/stx(+) strains from healthy and diarrheic calves for enterohemolysin production. In this study, the Premier EHEC assay was used to detect Shiga toxin antigens associated with eae/stx strains. When examined in the immunoassay, Shiga toxin antigens were detected for all 20 eae/stx (100%) strains from healthy calves and in 8 of 11 (73.0%) strains from diarrheic calves. Detection and characterization of the eae gene of shiga-like toxin-producing Escherichia coli using polymerase chain reaction Shiga toxin-producing Escherichia coli strains from bovines: association of adhesion with carriage of eae and other genes ./cache/cord-265765-zpf1bla8.txt ./txt/cord-265765-zpf1bla8.txt