id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-257572-dh4jvbmi	van Kasteren, Puck B.	In vivo assessment of equine arteritis virus vaccine improvement by disabling the deubiquitinase activity of papain-like protease 2	2015-07-09		.txt	text/plain	4711	255	51	Both viruses have been thoroughly characterized in cell[ 6 _ T D $ D I F F ] culture experiments, revealing no differences in replication kinetics, yet a strongly decreased DUB activity and an enhanced induction of interferon beta mRNA expression (a hallmark of innate immune activation) of the mutant virus compared to its parental counterpart (van Kasteren et al., 2013) . Upon challenge with the[ 4 _ T D $ D I F F ] virulent EAV KY84 strain, both nonvaccinated control horses (Group 3) showed virus replication, with amounts of viral RNA reaching levels comparable to those produced by the vaccine viruses (Fig. 3A) . We have previously shown that mutant EAV lacking PLP2 DUB activity induces a more potent innate immune response than its DUB-competent parental virus upon infection in cell culture (van Kasteren et al., 2013) , and hypothesized that this feature might be included in an improved arterivirus vaccine.	./cache/cord-257572-dh4jvbmi.txt	./txt/cord-257572-dh4jvbmi.txt