id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-272292-k0ugjb6f	Liu, Shih-Jen	Immunological characterizations of the nucleocapsid protein based SARS vaccine candidates	2006-04-12		.txt	text/plain	4957	261	57	The recombinant nucleocapsid (rN) protein of the coronavirus (CoV) responsible for severe acute respiratory syndrome (SARS) was cloned and expressed in Escherichia coli, extracted from cell lysates containing 6 M urea, then purified by Ni(2+)-affinity chromatography. To identify the B-cell immunodominant epitopes of the rN protein in the mouse and monkey, the reactivities of antisera raised against purified rN proteins formulated in ISA-51/CpG were tested with a panel of overlapping synthetic peptides covering the entire N protein sequence. We also only observed that peptides corresponding to residues 336–350 were capable of stimulating IFN-γ production in T-cell cultures derived from peripheral blood mononuclear cells (PBMCs) of macaques immunized with the rN protein emulsified in ISA/CpG adjuvant.	./cache/cord-272292-k0ugjb6f.txt	./txt/cord-272292-k0ugjb6f.txt