id	sid	tid	token	lemma	pos
cord-002791-tqchfdmy	1	1	key	key	JJ
cord-002791-tqchfdmy	1	2	:	:	:
cord-002791-tqchfdmy	1	3	cord-002791-tqchfdmy	cord-002791-tqchfdmy	.
cord-002791-tqchfdmy	2	1	authors	author	NNS
cord-002791-tqchfdmy	2	2	:	:	:
cord-002791-tqchfdmy	2	3	Mikel	Mikel	NNP
cord-002791-tqchfdmy	2	4	,	,	,
cord-002791-tqchfdmy	2	5	Pavel	Pavel	NNP
cord-002791-tqchfdmy	2	6	;	;	:
cord-002791-tqchfdmy	2	7	Vasickova	Vasickova	NNP
cord-002791-tqchfdmy	2	8	,	,	,
cord-002791-tqchfdmy	2	9	Petra	Petra	NNP
cord-002791-tqchfdmy	2	10	;	;	:
cord-002791-tqchfdmy	2	11	Kralik	Kralik	NNP
cord-002791-tqchfdmy	2	12	,	,	,
cord-002791-tqchfdmy	3	1	Petr	Petr	NNP
cord-002791-tqchfdmy	3	2	title	title	NN
cord-002791-tqchfdmy	3	3	:	:	:
cord-002791-tqchfdmy	3	4	One	one	CD
cord-002791-tqchfdmy	3	5	-	-	HYPH
cord-002791-tqchfdmy	3	6	plasmid	plasmid	NN
cord-002791-tqchfdmy	3	7	double	double	JJ
cord-002791-tqchfdmy	3	8	-	-	HYPH
cord-002791-tqchfdmy	3	9	expression	expression	NN
cord-002791-tqchfdmy	3	10	His	-PRON-	PRP$
cord-002791-tqchfdmy	3	11	-	-	HYPH
cord-002791-tqchfdmy	3	12	tag	tag	NN
cord-002791-tqchfdmy	3	13	system	system	NN
cord-002791-tqchfdmy	3	14	for	for	IN
cord-002791-tqchfdmy	3	15	rapid	rapid	JJ
cord-002791-tqchfdmy	3	16	production	production	NN
cord-002791-tqchfdmy	3	17	and	and	CC
cord-002791-tqchfdmy	3	18	easy	easy	JJ
cord-002791-tqchfdmy	3	19	purification	purification	NN
cord-002791-tqchfdmy	3	20	of	of	IN
cord-002791-tqchfdmy	3	21	MS2	MS2	NNP
cord-002791-tqchfdmy	3	22	phage	phage	NN
cord-002791-tqchfdmy	3	23	-	-	HYPH
cord-002791-tqchfdmy	3	24	like	like	JJ
cord-002791-tqchfdmy	3	25	particles	particle	NNS
cord-002791-tqchfdmy	3	26	date	date	NN
cord-002791-tqchfdmy	3	27	:	:	:
cord-002791-tqchfdmy	3	28	2017	2017	CD
cord-002791-tqchfdmy	3	29	-	-	HYPH
cord-002791-tqchfdmy	3	30	12	12	CD
cord-002791-tqchfdmy	3	31	-	-	SYM
cord-002791-tqchfdmy	3	32	13	13	CD
cord-002791-tqchfdmy	3	33	journal	journal	NN
cord-002791-tqchfdmy	3	34	:	:	:
cord-002791-tqchfdmy	4	1	Sci	Sci	NNP
cord-002791-tqchfdmy	4	2	Rep	Rep	NNP
cord-002791-tqchfdmy	4	3	DOI	DOI	NNP
cord-002791-tqchfdmy	4	4	:	:	:
cord-002791-tqchfdmy	5	1	10.1038	10.1038	CD
cord-002791-tqchfdmy	5	2	/	/	SYM
cord-002791-tqchfdmy	5	3	s41598	s41598	CD
cord-002791-tqchfdmy	5	4	-	-	HYPH
cord-002791-tqchfdmy	5	5	017	017	CD
cord-002791-tqchfdmy	5	6	-	-	HYPH
cord-002791-tqchfdmy	5	7	17951	17951	CD
cord-002791-tqchfdmy	5	8	-	-	SYM
cord-002791-tqchfdmy	5	9	5	5	CD
cord-002791-tqchfdmy	5	10	sha	sha	NN
cord-002791-tqchfdmy	5	11	:	:	:
cord-002791-tqchfdmy	5	12	637c82c1bb56fb9d7ff5a5a2b1c9645a80180065	637c82c1bb56fb9d7ff5a5a2b1c9645a80180065	CD
cord-002791-tqchfdmy	5	13	doc_id	doc_id	CD
cord-002791-tqchfdmy	5	14	:	:	:
cord-002791-tqchfdmy	5	15	2791	2791	CD
cord-002791-tqchfdmy	5	16	cord_uid	cord_uid	NNS
cord-002791-tqchfdmy	5	17	:	:	:
cord-002791-tqchfdmy	6	1	tqchfdmy	tqchfdmy	NNP
cord-002791-tqchfdmy	6	2	MS2	MS2	NNP
cord-002791-tqchfdmy	6	3	phage	phage	NN
cord-002791-tqchfdmy	6	4	-	-	HYPH
cord-002791-tqchfdmy	6	5	like	like	JJ
cord-002791-tqchfdmy	6	6	particles	particle	NNS
cord-002791-tqchfdmy	7	1	(	(	-LRB-
cord-002791-tqchfdmy	7	2	MS2	MS2	NNP
cord-002791-tqchfdmy	7	3	PLP	PLP	NNP
cord-002791-tqchfdmy	7	4	)	)	-RRB-
cord-002791-tqchfdmy	7	5	are	be	VBP
cord-002791-tqchfdmy	7	6	artificially	artificially	RB
cord-002791-tqchfdmy	7	7	constructed	construct	VBN
cord-002791-tqchfdmy	7	8	pseudo	pseudo	JJ
cord-002791-tqchfdmy	7	9	-	-	HYPH
cord-002791-tqchfdmy	7	10	viral	viral	JJ
cord-002791-tqchfdmy	7	11	particles	particle	NNS
cord-002791-tqchfdmy	7	12	derived	derive	VBN
cord-002791-tqchfdmy	7	13	from	from	IN
cord-002791-tqchfdmy	7	14	bacteriophage	bacteriophage	NN
cord-002791-tqchfdmy	7	15	MS2	MS2	NNP
cord-002791-tqchfdmy	7	16	.	.	.
cord-002791-tqchfdmy	8	1	They	-PRON-	PRP
cord-002791-tqchfdmy	8	2	are	be	VBP
cord-002791-tqchfdmy	8	3	able	able	JJ
cord-002791-tqchfdmy	8	4	to	to	TO
cord-002791-tqchfdmy	8	5	carry	carry	VB
cord-002791-tqchfdmy	8	6	a	a	DT
cord-002791-tqchfdmy	8	7	specific	specific	JJ
cord-002791-tqchfdmy	8	8	single	single	JJ
cord-002791-tqchfdmy	8	9	stranded	strand	VBN
cord-002791-tqchfdmy	8	10	RNA	RNA	NNP
cord-002791-tqchfdmy	8	11	(	(	-LRB-
cord-002791-tqchfdmy	8	12	ssRNA	ssrna	NN
cord-002791-tqchfdmy	8	13	)	)	-RRB-
cord-002791-tqchfdmy	8	14	sequence	sequence	NN
cord-002791-tqchfdmy	8	15	of	of	IN
cord-002791-tqchfdmy	8	16	choice	choice	NN
cord-002791-tqchfdmy	8	17	inside	inside	IN
cord-002791-tqchfdmy	8	18	their	-PRON-	PRP$
cord-002791-tqchfdmy	8	19	capsid	capsid	NN
cord-002791-tqchfdmy	8	20	,	,	,
cord-002791-tqchfdmy	8	21	thus	thus	RB
cord-002791-tqchfdmy	8	22	protecting	protect	VBG
cord-002791-tqchfdmy	8	23	it	-PRON-	PRP
cord-002791-tqchfdmy	8	24	against	against	IN
cord-002791-tqchfdmy	8	25	the	the	DT
cord-002791-tqchfdmy	8	26	effects	effect	NNS
cord-002791-tqchfdmy	8	27	of	of	IN
cord-002791-tqchfdmy	8	28	ubiquitous	ubiquitous	JJ
cord-002791-tqchfdmy	8	29	nucleases	nuclease	NNS
cord-002791-tqchfdmy	8	30	.	.	.
cord-002791-tqchfdmy	9	1	Such	such	JJ
cord-002791-tqchfdmy	9	2	particles	particle	NNS
cord-002791-tqchfdmy	9	3	are	be	VBP
cord-002791-tqchfdmy	9	4	able	able	JJ
cord-002791-tqchfdmy	9	5	to	to	TO
cord-002791-tqchfdmy	9	6	mimic	mimic	VB
cord-002791-tqchfdmy	9	7	ssRNA	ssrna	NN
cord-002791-tqchfdmy	9	8	viruses	virus	NNS
cord-002791-tqchfdmy	9	9	and	and	CC
cord-002791-tqchfdmy	9	10	,	,	,
cord-002791-tqchfdmy	9	11	thus	thus	RB
cord-002791-tqchfdmy	9	12	,	,	,
cord-002791-tqchfdmy	9	13	may	may	MD
cord-002791-tqchfdmy	9	14	serve	serve	VB
cord-002791-tqchfdmy	9	15	as	as	IN
cord-002791-tqchfdmy	9	16	the	the	DT
cord-002791-tqchfdmy	9	17	process	process	NN
cord-002791-tqchfdmy	9	18	control	control	NN
cord-002791-tqchfdmy	9	19	for	for	IN
cord-002791-tqchfdmy	9	20	molecular	molecular	JJ
cord-002791-tqchfdmy	9	21	detection	detection	NN
cord-002791-tqchfdmy	9	22	and	and	CC
cord-002791-tqchfdmy	9	23	quantification	quantification	NN
cord-002791-tqchfdmy	9	24	of	of	IN
cord-002791-tqchfdmy	9	25	such	such	JJ
cord-002791-tqchfdmy	9	26	agents	agent	NNS
cord-002791-tqchfdmy	9	27	in	in	IN
cord-002791-tqchfdmy	9	28	several	several	JJ
cord-002791-tqchfdmy	9	29	kinds	kind	NNS
cord-002791-tqchfdmy	9	30	of	of	IN
cord-002791-tqchfdmy	9	31	matrices	matrix	NNS
cord-002791-tqchfdmy	9	32	,	,	,
cord-002791-tqchfdmy	9	33	vaccines	vaccine	NNS
cord-002791-tqchfdmy	9	34	and	and	CC
cord-002791-tqchfdmy	9	35	vaccine	vaccine	NN
cord-002791-tqchfdmy	9	36	candidates	candidate	NNS
cord-002791-tqchfdmy	9	37	,	,	,
cord-002791-tqchfdmy	9	38	drug	drug	NN
cord-002791-tqchfdmy	9	39	delivery	delivery	NN
cord-002791-tqchfdmy	9	40	systems	system	NNS
cord-002791-tqchfdmy	9	41	,	,	,
cord-002791-tqchfdmy	9	42	and	and	CC
cord-002791-tqchfdmy	9	43	systems	system	NNS
cord-002791-tqchfdmy	9	44	for	for	IN
cord-002791-tqchfdmy	9	45	the	the	DT
cord-002791-tqchfdmy	9	46	display	display	NN
cord-002791-tqchfdmy	9	47	of	of	IN
cord-002791-tqchfdmy	9	48	immunologically	immunologically	RB
cord-002791-tqchfdmy	9	49	active	active	JJ
cord-002791-tqchfdmy	9	50	peptides	peptide	NNS
cord-002791-tqchfdmy	9	51	or	or	CC
cord-002791-tqchfdmy	9	52	nanomachines	nanomachine	NNS
cord-002791-tqchfdmy	9	53	.	.	.
cord-002791-tqchfdmy	10	1	Currently	currently	RB
cord-002791-tqchfdmy	10	2	,	,	,
cord-002791-tqchfdmy	10	3	there	there	EX
cord-002791-tqchfdmy	10	4	are	be	VBP
cord-002791-tqchfdmy	10	5	several	several	JJ
cord-002791-tqchfdmy	10	6	different	different	JJ
cord-002791-tqchfdmy	10	7	in	in	IN
cord-002791-tqchfdmy	10	8	vivo	vivo	NN
cord-002791-tqchfdmy	10	9	plasmid	plasmid	NN
cord-002791-tqchfdmy	10	10	-	-	HYPH
cord-002791-tqchfdmy	10	11	driven	drive	VBN
cord-002791-tqchfdmy	10	12	packaging	packaging	NN
cord-002791-tqchfdmy	10	13	systems	system	NNS
cord-002791-tqchfdmy	10	14	for	for	IN
cord-002791-tqchfdmy	10	15	production	production	NN
cord-002791-tqchfdmy	10	16	of	of	IN
cord-002791-tqchfdmy	10	17	MS2	MS2	NNP
cord-002791-tqchfdmy	10	18	PLP	PLP	NNP
cord-002791-tqchfdmy	10	19	.	.	.
cord-002791-tqchfdmy	11	1	In	in	IN
cord-002791-tqchfdmy	11	2	order	order	NN
cord-002791-tqchfdmy	11	3	to	to	TO
cord-002791-tqchfdmy	11	4	combine	combine	VB
cord-002791-tqchfdmy	11	5	all	all	PDT
cord-002791-tqchfdmy	11	6	the	the	DT
cord-002791-tqchfdmy	11	7	advantages	advantage	NNS
cord-002791-tqchfdmy	11	8	of	of	IN
cord-002791-tqchfdmy	11	9	the	the	DT
cord-002791-tqchfdmy	11	10	available	available	JJ
cord-002791-tqchfdmy	11	11	systems	system	NNS
cord-002791-tqchfdmy	11	12	and	and	CC
cord-002791-tqchfdmy	11	13	to	to	TO
cord-002791-tqchfdmy	11	14	upgrade	upgrade	VB
cord-002791-tqchfdmy	11	15	and	and	CC
cord-002791-tqchfdmy	11	16	simplify	simplify	VB
cord-002791-tqchfdmy	11	17	the	the	DT
cord-002791-tqchfdmy	11	18	production	production	NN
cord-002791-tqchfdmy	11	19	and	and	CC
cord-002791-tqchfdmy	11	20	purification	purification	NN
cord-002791-tqchfdmy	11	21	of	of	IN
cord-002791-tqchfdmy	11	22	MS2	MS2	NNP
cord-002791-tqchfdmy	11	23	PLP	PLP	NNP
cord-002791-tqchfdmy	11	24	,	,	,
cord-002791-tqchfdmy	11	25	a	a	DT
cord-002791-tqchfdmy	11	26	one	one	CD
cord-002791-tqchfdmy	11	27	-	-	HYPH
cord-002791-tqchfdmy	11	28	plasmid	plasmid	NN
cord-002791-tqchfdmy	11	29	double	double	JJ
cord-002791-tqchfdmy	11	30	-	-	HYPH
cord-002791-tqchfdmy	11	31	expression	expression	NN
cord-002791-tqchfdmy	11	32	His	-PRON-	PRP$
cord-002791-tqchfdmy	11	33	-	-	HYPH
cord-002791-tqchfdmy	11	34	tag	tag	NN
cord-002791-tqchfdmy	11	35	system	system	NN
cord-002791-tqchfdmy	11	36	was	be	VBD
cord-002791-tqchfdmy	11	37	designed	design	VBN
cord-002791-tqchfdmy	11	38	.	.	.
cord-002791-tqchfdmy	12	1	The	the	DT
cord-002791-tqchfdmy	12	2	described	describe	VBN
cord-002791-tqchfdmy	12	3	system	system	NN
cord-002791-tqchfdmy	12	4	utilizes	utilize	VBZ
cord-002791-tqchfdmy	12	5	a	a	DT
cord-002791-tqchfdmy	12	6	unique	unique	JJ
cord-002791-tqchfdmy	12	7	fusion	fusion	NN
cord-002791-tqchfdmy	12	8	insertional	insertional	JJ
cord-002791-tqchfdmy	12	9	mutation	mutation	NN
cord-002791-tqchfdmy	12	10	enabling	enable	VBG
cord-002791-tqchfdmy	12	11	purification	purification	NN
cord-002791-tqchfdmy	12	12	of	of	IN
cord-002791-tqchfdmy	12	13	particles	particle	NNS
cord-002791-tqchfdmy	12	14	using	use	VBG
cord-002791-tqchfdmy	12	15	His	-PRON-	PRP$
cord-002791-tqchfdmy	12	16	-	-	HYPH
cord-002791-tqchfdmy	12	17	tag	tag	NN
cord-002791-tqchfdmy	12	18	affinity	affinity	NN
cord-002791-tqchfdmy	12	19	.	.	.
cord-002791-tqchfdmy	13	1	Using	use	VBG
cord-002791-tqchfdmy	13	2	this	this	DT
cord-002791-tqchfdmy	13	3	new	new	JJ
cord-002791-tqchfdmy	13	4	production	production	NN
cord-002791-tqchfdmy	13	5	system	system	NN
cord-002791-tqchfdmy	13	6	,	,	,
cord-002791-tqchfdmy	13	7	highly	highly	RB
cord-002791-tqchfdmy	13	8	pure	pure	JJ
cord-002791-tqchfdmy	13	9	MS2	MS2	NNP
cord-002791-tqchfdmy	13	10	PLP	PLP	NNP
cord-002791-tqchfdmy	13	11	can	can	MD
cord-002791-tqchfdmy	13	12	be	be	VB
cord-002791-tqchfdmy	13	13	quickly	quickly	RB
cord-002791-tqchfdmy	13	14	produced	produce	VBN
cord-002791-tqchfdmy	13	15	and	and	CC
cord-002791-tqchfdmy	13	16	purified	purify	VBN
cord-002791-tqchfdmy	13	17	by	by	IN
cord-002791-tqchfdmy	13	18	a	a	DT
cord-002791-tqchfdmy	13	19	fast	fast	JJ
cord-002791-tqchfdmy	13	20	performance	performance	NN
cord-002791-tqchfdmy	13	21	liquid	liquid	NN
cord-002791-tqchfdmy	13	22	chromatography	chromatography	NN
cord-002791-tqchfdmy	13	23	(	(	-LRB-
cord-002791-tqchfdmy	13	24	FPLC	FPLC	NNP
cord-002791-tqchfdmy	13	25	)	)	-RRB-
cord-002791-tqchfdmy	13	26	approach	approach	NN
cord-002791-tqchfdmy	13	27	.	.	.
cord-002791-tqchfdmy	14	1	The	the	DT
cord-002791-tqchfdmy	14	2	system	system	NN
cord-002791-tqchfdmy	14	3	can	can	MD
cord-002791-tqchfdmy	14	4	be	be	VB
cord-002791-tqchfdmy	14	5	easily	easily	RB
cord-002791-tqchfdmy	14	6	adapted	adapt	VBN
cord-002791-tqchfdmy	14	7	to	to	TO
cord-002791-tqchfdmy	14	8	produce	produce	VB
cord-002791-tqchfdmy	14	9	other	other	JJ
cord-002791-tqchfdmy	14	10	MS2	MS2	NNP
cord-002791-tqchfdmy	14	11	PLP	PLP	NNP
cord-002791-tqchfdmy	14	12	with	with	IN
cord-002791-tqchfdmy	14	13	different	different	JJ
cord-002791-tqchfdmy	14	14	properties	property	NNS
cord-002791-tqchfdmy	14	15	.	.	.
cord-002791-tqchfdmy	14	16	.	.	.
cord-002791-tqchfdmy	15	1	Diagram	Diagram	NNP
cord-002791-tqchfdmy	15	2	of	of	IN
cord-002791-tqchfdmy	15	3	cloning	clone	VBG
cord-002791-tqchfdmy	15	4	experiments	experiment	NNS
cord-002791-tqchfdmy	15	5	in	in	IN
cord-002791-tqchfdmy	15	6	this	this	DT
cord-002791-tqchfdmy	15	7	study	study	NN
cord-002791-tqchfdmy	15	8	.	.	.
cord-002791-tqchfdmy	16	1	A	a	DT
cord-002791-tqchfdmy	16	2	specific	specific	JJ
cord-002791-tqchfdmy	16	3	control	control	NN
cord-002791-tqchfdmy	16	4	sequence	sequence	NN
cord-002791-tqchfdmy	16	5	derived	derive	VBN
cord-002791-tqchfdmy	16	6	from	from	IN
cord-002791-tqchfdmy	16	7	the	the	DT
cord-002791-tqchfdmy	16	8	mitochondrial	mitochondrial	JJ
cord-002791-tqchfdmy	16	9	DNA	dna	NN
cord-002791-tqchfdmy	16	10	(	(	-LRB-
cord-002791-tqchfdmy	16	11	mtDNA	mtDNA	NNP
cord-002791-tqchfdmy	16	12	)	)	-RRB-
cord-002791-tqchfdmy	17	1	sequences	sequence	NNS
cord-002791-tqchfdmy	17	2	of	of	IN
cord-002791-tqchfdmy	17	3	two	two	CD
cord-002791-tqchfdmy	17	4	extinct	extinct	JJ
cord-002791-tqchfdmy	17	5	species	specie	NNS
cord-002791-tqchfdmy	17	6	with	with	IN
cord-002791-tqchfdmy	17	7	one	one	CD
cord-002791-tqchfdmy	17	8	C	c	NN
cord-002791-tqchfdmy	17	9	-	-	HYPH
cord-002791-tqchfdmy	17	10	variant	variant	NN
cord-002791-tqchfdmy	17	11	pac	pac	NNP
cord-002791-tqchfdmy	17	12	-	-	HYPH
cord-002791-tqchfdmy	17	13	site	site	NN
cord-002791-tqchfdmy	17	14	(	(	-LRB-
cord-002791-tqchfdmy	17	15	TMpac	TMpac	NNP
cord-002791-tqchfdmy	17	16	)	)	-RRB-
cord-002791-tqchfdmy	17	17	was	be	VBD
cord-002791-tqchfdmy	17	18	cloned	clone	VBN
cord-002791-tqchfdmy	17	19	into	into	IN
cord-002791-tqchfdmy	17	20	the	the	DT
cord-002791-tqchfdmy	17	21	multiple	multiple	JJ
cord-002791-tqchfdmy	17	22	cloning	cloning	NN
cord-002791-tqchfdmy	17	23	site	site	NN
cord-002791-tqchfdmy	17	24	2	2	CD
cord-002791-tqchfdmy	17	25	(	(	-LRB-
cord-002791-tqchfdmy	17	26	MCS2	MCS2	NNP
cord-002791-tqchfdmy	17	27	)	)	-RRB-
cord-002791-tqchfdmy	17	28	of	of	IN
cord-002791-tqchfdmy	17	29	the	the	DT
cord-002791-tqchfdmy	17	30	pACYCDuet-1	pacycduet-1	JJ
cord-002791-tqchfdmy	17	31	vector	vector	NN
cord-002791-tqchfdmy	17	32	(	(	-LRB-
cord-002791-tqchfdmy	17	33	Novagen	Novagen	NNP
cord-002791-tqchfdmy	17	34	,	,	,
cord-002791-tqchfdmy	17	35	Merck	Merck	NNP
cord-002791-tqchfdmy	17	36	,	,	,
cord-002791-tqchfdmy	17	37	Germany	Germany	NNP
cord-002791-tqchfdmy	17	38	)	)	-RRB-
cord-002791-tqchfdmy	17	39	to	to	TO
cord-002791-tqchfdmy	17	40	construct	construct	VB
cord-002791-tqchfdmy	17	41	the	the	DT
cord-002791-tqchfdmy	17	42	pACYCDuet-1-TM	pacycduet-1-tm	CD
cord-002791-tqchfdmy	17	43	vector	vector	NN
cord-002791-tqchfdmy	17	44	.	.	.
cord-002791-tqchfdmy	18	1	Then	then	RB
cord-002791-tqchfdmy	18	2	,	,	,
cord-002791-tqchfdmy	18	3	the	the	DT
cord-002791-tqchfdmy	18	4	fragment	fragment	NN
cord-002791-tqchfdmy	18	5	of	of	IN
cord-002791-tqchfdmy	18	6	maturase	maturase	NN
cord-002791-tqchfdmy	18	7	and	and	CC
cord-002791-tqchfdmy	18	8	single	single	JJ
cord-002791-tqchfdmy	18	9	-	-	HYPH
cord-002791-tqchfdmy	18	10	chain	chain	NN
cord-002791-tqchfdmy	18	11	version	version	NN
cord-002791-tqchfdmy	18	12	of	of	IN
cord-002791-tqchfdmy	18	13	the	the	DT
cord-002791-tqchfdmy	18	14	coat	coat	NN
cord-002791-tqchfdmy	18	15	protein	protein	NN
cord-002791-tqchfdmy	18	16	dimer	dimer	NN
cord-002791-tqchfdmy	18	17	containing	contain	VBG
cord-002791-tqchfdmy	18	18	the	the	DT
cord-002791-tqchfdmy	18	19	His	-PRON-	PRP$
cord-002791-tqchfdmy	18	20	-	-	HYPH
cord-002791-tqchfdmy	18	21	tag	tag	NN
cord-002791-tqchfdmy	18	22	was	be	VBD
cord-002791-tqchfdmy	18	23	cloned	clone	VBN
cord-002791-tqchfdmy	18	24	into	into	IN
cord-002791-tqchfdmy	18	25	the	the	DT
cord-002791-tqchfdmy	18	26	multiple	multiple	JJ
cord-002791-tqchfdmy	18	27	cloning	cloning	NN
cord-002791-tqchfdmy	18	28	site	site	NN
cord-002791-tqchfdmy	18	29	1	1	CD
cord-002791-tqchfdmy	18	30	(	(	-LRB-
cord-002791-tqchfdmy	18	31	MCS1	MCS1	NNP
cord-002791-tqchfdmy	18	32	)	)	-RRB-
cord-002791-tqchfdmy	18	33	of	of	IN
cord-002791-tqchfdmy	18	34	the	the	DT
cord-002791-tqchfdmy	18	35	pACYCDuet-1-TM	pacycduet-1-tm	JJ
cord-002791-tqchfdmy	18	36	to	to	TO
cord-002791-tqchfdmy	18	37	create	create	VB
cord-002791-tqchfdmy	18	38	the	the	DT
cord-002791-tqchfdmy	18	39	pACYCDuet-1-TM	pACYCDuet-1-TM	VBN
cord-002791-tqchfdmy	18	40	-	-	HYPH
cord-002791-tqchfdmy	18	41	CoatDimer	CoatDimer	NNP
cord-002791-tqchfdmy	18	42	-	-	:
cord-002791-tqchfdmy	18	43	His	-PRON-	PRP$
cord-002791-tqchfdmy	18	44	vector	vector	NN
cord-002791-tqchfdmy	18	45	.	.	.
cord-002791-tqchfdmy	19	1	Scientific	Scientific	NNP
cord-002791-tqchfdmy	19	2	RepoRts	RepoRts	NNP
cord-002791-tqchfdmy	19	3	|	|	NNP
cord-002791-tqchfdmy	19	4	7:17501	7:17501	.
cord-002791-tqchfdmy	20	1	|	|	NNP
cord-002791-tqchfdmy	20	2	https://doi.org/10.1038/s41598-017-17951-5	https://doi.org/10.1038/s41598-017-17951-5	NNP
cord-002791-tqchfdmy	20	3	Induction	Induction	NNP
cord-002791-tqchfdmy	20	4	of	of	IN
cord-002791-tqchfdmy	20	5	expression	expression	NN
cord-002791-tqchfdmy	20	6	of	of	IN
cord-002791-tqchfdmy	20	7	His	-PRON-	PRP$
cord-002791-tqchfdmy	20	8	-	-	HYPH
cord-002791-tqchfdmy	20	9	tagged	tag	VBN
cord-002791-tqchfdmy	20	10	MS2	MS2	NNP
cord-002791-tqchfdmy	20	11	PLP	PLP	NNP
cord-002791-tqchfdmy	20	12	.	.	.
cord-002791-tqchfdmy	21	1	The	the	DT
cord-002791-tqchfdmy	21	2	pACYCDuet-1-TM	pacycduet-1-tm	JJ
cord-002791-tqchfdmy	21	3	-	-	HYPH
cord-002791-tqchfdmy	21	4	CoatDimer	CoatDimer	NNP
cord-002791-tqchfdmy	21	5	-	-	HYPH
cord-002791-tqchfdmy	21	6	His	-PRON-	PRP$
cord-002791-tqchfdmy	21	7	plasmid	plasmid	NN
cord-002791-tqchfdmy	21	8	was	be	VBD
cord-002791-tqchfdmy	21	9	transformed	transform	VBN
cord-002791-tqchfdmy	21	10	into	into	IN
cord-002791-tqchfdmy	21	11	E.	E.	NNP
cord-002791-tqchfdmy	21	12	coli	coli	NNS
cord-002791-tqchfdmy	21	13	LB21	LB21	NNP
cord-002791-tqchfdmy	21	14	(	(	-LRB-
cord-002791-tqchfdmy	21	15	DE3	DE3	NNP
cord-002791-tqchfdmy	21	16	)	)	-RRB-
cord-002791-tqchfdmy	21	17	cells	cell	NNS
cord-002791-tqchfdmy	21	18	and	and	CC
cord-002791-tqchfdmy	21	19	the	the	DT
cord-002791-tqchfdmy	21	20	production	production	NN
cord-002791-tqchfdmy	21	21	of	of	IN
cord-002791-tqchfdmy	21	22	His	-PRON-	PRP$
cord-002791-tqchfdmy	21	23	-	-	HYPH
cord-002791-tqchfdmy	21	24	tagged	tag	VBN
cord-002791-tqchfdmy	21	25	MS2	MS2	NNP
cord-002791-tqchfdmy	21	26	PLP	PLP	NNP
cord-002791-tqchfdmy	21	27	was	be	VBD
cord-002791-tqchfdmy	21	28	induced	induce	VBN
cord-002791-tqchfdmy	21	29	as	as	IN
cord-002791-tqchfdmy	21	30	described	describe	VBN
cord-002791-tqchfdmy	21	31	below	below	RB
cord-002791-tqchfdmy	21	32	.	.	.
cord-002791-tqchfdmy	22	1	Purification	purification	NN
cord-002791-tqchfdmy	22	2	of	of	IN
cord-002791-tqchfdmy	22	3	His	-PRON-	PRP$
cord-002791-tqchfdmy	22	4	-	-	HYPH
cord-002791-tqchfdmy	22	5	tagged	tag	VBN
cord-002791-tqchfdmy	22	6	MS2	MS2	NNP
cord-002791-tqchfdmy	22	7	PLP	PLP	NNP
cord-002791-tqchfdmy	22	8	.	.	.
cord-002791-tqchfdmy	23	1	His	-PRON-	PRP$
cord-002791-tqchfdmy	23	2	-	-	HYPH
cord-002791-tqchfdmy	23	3	tagged	tag	VBN
cord-002791-tqchfdmy	23	4	MS2	MS2	NNP
cord-002791-tqchfdmy	23	5	PLP	PLP	NNP
cord-002791-tqchfdmy	23	6	were	be	VBD
cord-002791-tqchfdmy	23	7	purified	purify	VBN
cord-002791-tqchfdmy	23	8	as	as	IN
cord-002791-tqchfdmy	23	9	described	describe	VBN
cord-002791-tqchfdmy	23	10	below	below	IN
cord-002791-tqchfdmy	23	11	using	use	VBG
cord-002791-tqchfdmy	23	12	an	an	DT
cord-002791-tqchfdmy	23	13	FPLC	FPLC	NNP
cord-002791-tqchfdmy	23	14	approach	approach	NN
cord-002791-tqchfdmy	23	15	.	.	.
cord-002791-tqchfdmy	24	1	The	the	DT
cord-002791-tqchfdmy	24	2	integrity	integrity	NN
cord-002791-tqchfdmy	24	3	of	of	IN
cord-002791-tqchfdmy	24	4	His	-PRON-	PRP$
cord-002791-tqchfdmy	24	5	-	-	HYPH
cord-002791-tqchfdmy	24	6	tagged	tag	VBN
cord-002791-tqchfdmy	24	7	MS2	MS2	NNP
cord-002791-tqchfdmy	24	8	PLP	PLP	NNP
cord-002791-tqchfdmy	24	9	isolated	isolate	VBN
cord-002791-tqchfdmy	24	10	using	use	VBG
cord-002791-tqchfdmy	24	11	FPLC	FPLC	NNP
cord-002791-tqchfdmy	24	12	was	be	VBD
cord-002791-tqchfdmy	24	13	verified	verify	VBN
cord-002791-tqchfdmy	24	14	by	by	IN
cord-002791-tqchfdmy	24	15	TEM	TEM	NNP
cord-002791-tqchfdmy	24	16	(	(	-LRB-
cord-002791-tqchfdmy	24	17	Fig	Fig	NNP
cord-002791-tqchfdmy	24	18	.	.	.
cord-002791-tqchfdmy	24	19	3	3	CD
cord-002791-tqchfdmy	24	20	)	)	-RRB-
cord-002791-tqchfdmy	24	21	.	.	.
cord-002791-tqchfdmy	25	1	The	the	DT
cord-002791-tqchfdmy	25	2	TEM	tem	NN
cord-002791-tqchfdmy	25	3	images	image	NNS
cord-002791-tqchfdmy	25	4	clearly	clearly	RB
cord-002791-tqchfdmy	25	5	show	show	VBP
cord-002791-tqchfdmy	25	6	that	that	IN
cord-002791-tqchfdmy	25	7	the	the	DT
cord-002791-tqchfdmy	25	8	His	His	NNP
cord-002791-tqchfdmy	25	9	-	-	HYPH
cord-002791-tqchfdmy	25	10	tagged	tag	VBN
cord-002791-tqchfdmy	25	11	MS2	MS2	NNP
cord-002791-tqchfdmy	25	12	PLP	PLP	NNP
cord-002791-tqchfdmy	25	13	were	be	VBD
cord-002791-tqchfdmy	25	14	correctly	correctly	RB
cord-002791-tqchfdmy	25	15	assembled	assemble	VBN
cord-002791-tqchfdmy	25	16	into	into	IN
cord-002791-tqchfdmy	25	17	intact	intact	JJ
cord-002791-tqchfdmy	25	18	capsids	capsid	NNS
cord-002791-tqchfdmy	25	19	of	of	IN
cord-002791-tqchfdmy	25	20	approximately	approximately	RB
cord-002791-tqchfdmy	25	21	27	27	CD
cord-002791-tqchfdmy	25	22	nm	nm	NN
cord-002791-tqchfdmy	25	23	in	in	IN
cord-002791-tqchfdmy	25	24	diameter	diameter	NN
cord-002791-tqchfdmy	25	25	.	.	.
cord-002791-tqchfdmy	26	1	The	the	DT
cord-002791-tqchfdmy	26	2	His	His	NNP
cord-002791-tqchfdmy	26	3	-	-	HYPH
cord-002791-tqchfdmy	26	4	tagged	tag	VBN
cord-002791-tqchfdmy	26	5	MS2	MS2	NNP
cord-002791-tqchfdmy	26	6	PLP	PLP	NNP
cord-002791-tqchfdmy	26	7	were	be	VBD
cord-002791-tqchfdmy	26	8	not	not	RB
cord-002791-tqchfdmy	26	9	damaged	damage	VBN
cord-002791-tqchfdmy	26	10	by	by	IN
cord-002791-tqchfdmy	26	11	the	the	DT
cord-002791-tqchfdmy	26	12	purification	purification	NN
cord-002791-tqchfdmy	26	13	process	process	NN
cord-002791-tqchfdmy	26	14	,	,	,
cord-002791-tqchfdmy	26	15	were	be	VBD
cord-002791-tqchfdmy	26	16	isolated	isolate	VBN
cord-002791-tqchfdmy	26	17	in	in	IN
cord-002791-tqchfdmy	26	18	a	a	DT
cord-002791-tqchfdmy	26	19	large	large	JJ
cord-002791-tqchfdmy	26	20	quantity	quantity	NN
cord-002791-tqchfdmy	26	21	(	(	-LRB-
cord-002791-tqchfdmy	26	22	2	2	CD
cord-002791-tqchfdmy	26	23	×	×	NN
cord-002791-tqchfdmy	26	24	10	10	CD
cord-002791-tqchfdmy	26	25	10	10	CD
cord-002791-tqchfdmy	26	26	particles/µl	particles/µl	NN
cord-002791-tqchfdmy	26	27	;	;	:
cord-002791-tqchfdmy	26	28	RT	RT	NNP
cord-002791-tqchfdmy	26	29	-	-	HYPH
cord-002791-tqchfdmy	26	30	qPCR	qpcr	NN
cord-002791-tqchfdmy	26	31	quantification	quantification	NN
cord-002791-tqchfdmy	26	32	according	accord	VBG
cord-002791-tqchfdmy	26	33	to	to	IN
cord-002791-tqchfdmy	26	34	8	8	CD
cord-002791-tqchfdmy	26	35	)	)	-RRB-
cord-002791-tqchfdmy	26	36	,	,	,
cord-002791-tqchfdmy	26	37	and	and	CC
cord-002791-tqchfdmy	26	38	did	do	VBD
cord-002791-tqchfdmy	26	39	not	not	RB
cord-002791-tqchfdmy	26	40	form	form	VB
cord-002791-tqchfdmy	26	41	any	any	DT
cord-002791-tqchfdmy	26	42	aggregates	aggregate	NNS
cord-002791-tqchfdmy	26	43	(	(	-LRB-
cord-002791-tqchfdmy	26	44	Fig	Fig	NNP
cord-002791-tqchfdmy	26	45	.	.	.
cord-002791-tqchfdmy	26	46	3	3	CD
cord-002791-tqchfdmy	26	47	)	)	-RRB-
cord-002791-tqchfdmy	26	48	.	.	.
cord-002791-tqchfdmy	27	1	Characterization	characterization	NN
cord-002791-tqchfdmy	27	2	of	of	IN
cord-002791-tqchfdmy	27	3	the	the	DT
cord-002791-tqchfdmy	27	4	single	single	JJ
cord-002791-tqchfdmy	27	5	-	-	HYPH
cord-002791-tqchfdmy	27	6	chain	chain	NN
cord-002791-tqchfdmy	27	7	version	version	NN
cord-002791-tqchfdmy	27	8	of	of	IN
cord-002791-tqchfdmy	27	9	the	the	DT
cord-002791-tqchfdmy	27	10	coat	coat	NN
cord-002791-tqchfdmy	27	11	protein	protein	NN
cord-002791-tqchfdmy	27	12	dimer	dimer	NN
cord-002791-tqchfdmy	27	13	containing	contain	VBG
cord-002791-tqchfdmy	27	14	the	the	DT
cord-002791-tqchfdmy	27	15	His	His	NNP
cord-002791-tqchfdmy	27	16	-	-	HYPH
cord-002791-tqchfdmy	27	17	tag	tag	NN
cord-002791-tqchfdmy	27	18	.	.	.
cord-002791-tqchfdmy	28	1	The	the	DT
cord-002791-tqchfdmy	28	2	results	result	NNS
cord-002791-tqchfdmy	28	3	of	of	IN
cord-002791-tqchfdmy	28	4	sodium	sodium	NN
cord-002791-tqchfdmy	28	5	dodecyl	dodecyl	NNP
cord-002791-tqchfdmy	28	6	sulfate	sulfate	NN
cord-002791-tqchfdmy	28	7	-	-	HYPH
cord-002791-tqchfdmy	28	8	polyacrylamide	polyacrylamide	NN
cord-002791-tqchfdmy	28	9	gel	gel	NN
cord-002791-tqchfdmy	28	10	electrophoresis	electrophoresis	NN
cord-002791-tqchfdmy	28	11	(	(	-LRB-
cord-002791-tqchfdmy	28	12	SDS	SDS	NNP
cord-002791-tqchfdmy	28	13	-	-	HYPH
cord-002791-tqchfdmy	28	14	PAGE	PAGE	NNP
cord-002791-tqchfdmy	28	15	)	)	-RRB-
cord-002791-tqchfdmy	28	16	showed	show	VBD
cord-002791-tqchfdmy	28	17	that	that	IN
cord-002791-tqchfdmy	28	18	MS2	MS2	NNP
cord-002791-tqchfdmy	28	19	PLP	PLP	NNP
cord-002791-tqchfdmy	28	20	contained	contain	VBD
cord-002791-tqchfdmy	28	21	wild	wild	JJ
cord-002791-tqchfdmy	28	22	-	-	HYPH
cord-002791-tqchfdmy	28	23	type	type	NN
cord-002791-tqchfdmy	28	24	coat	coat	NN
cord-002791-tqchfdmy	28	25	protein	protein	NN
cord-002791-tqchfdmy	28	26	with	with	IN
cord-002791-tqchfdmy	28	27	an	an	DT
cord-002791-tqchfdmy	28	28	approximate	approximate	JJ
cord-002791-tqchfdmy	28	29	molecular	molecular	JJ
cord-002791-tqchfdmy	28	30	weight	weight	NN
cord-002791-tqchfdmy	28	31	of	of	IN
cord-002791-tqchfdmy	28	32	13	13	CD
cord-002791-tqchfdmy	28	33	kDa	kDa	NNS
cord-002791-tqchfdmy	28	34	(	(	-LRB-
cord-002791-tqchfdmy	28	35	Fig	Fig	NNP
cord-002791-tqchfdmy	28	36	.	.	.
cord-002791-tqchfdmy	28	37	4,A1	4,a1	CD
cord-002791-tqchfdmy	28	38	)	)	-RRB-
cord-002791-tqchfdmy	28	39	,	,	,
cord-002791-tqchfdmy	28	40	whereas	whereas	IN
cord-002791-tqchfdmy	28	41	His	-PRON-	PRP$
cord-002791-tqchfdmy	28	42	-	-	HYPH
cord-002791-tqchfdmy	28	43	tagged	tag	VBN
cord-002791-tqchfdmy	28	44	MS2	MS2	NNP
cord-002791-tqchfdmy	28	45	PLP	PLP	NNP
cord-002791-tqchfdmy	28	46	incorporated	incorporate	VBD
cord-002791-tqchfdmy	28	47	a	a	DT
cord-002791-tqchfdmy	28	48	single	single	JJ
cord-002791-tqchfdmy	28	49	-	-	HYPH
cord-002791-tqchfdmy	28	50	chain	chain	NN
cord-002791-tqchfdmy	28	51	version	version	NN
cord-002791-tqchfdmy	28	52	of	of	IN
cord-002791-tqchfdmy	28	53	the	the	DT
cord-002791-tqchfdmy	28	54	coat	coat	NN
cord-002791-tqchfdmy	28	55	protein	protein	NN
cord-002791-tqchfdmy	28	56	dimer	dimer	NNP
cord-002791-tqchfdmy	28	57	containing	contain	VBG
cord-002791-tqchfdmy	28	58	a	a	DT
cord-002791-tqchfdmy	28	59	His	-PRON-	PRP$
cord-002791-tqchfdmy	28	60	-	-	HYPH
cord-002791-tqchfdmy	28	61	tag	tag	NN
cord-002791-tqchfdmy	28	62	with	with	IN
cord-002791-tqchfdmy	28	63	an	an	DT
cord-002791-tqchfdmy	28	64	approximate	approximate	JJ
cord-002791-tqchfdmy	28	65	weight	weight	NN
cord-002791-tqchfdmy	28	66	of	of	IN
cord-002791-tqchfdmy	28	67	29	29	CD
cord-002791-tqchfdmy	28	68	kDa	kDa	NNS
cord-002791-tqchfdmy	28	69	(	(	-LRB-
cord-002791-tqchfdmy	28	70	Fig	Fig	NNP
cord-002791-tqchfdmy	28	71	.	.	.
cord-002791-tqchfdmy	28	72	4,A2	4,a2	CD
cord-002791-tqchfdmy	28	73	)	)	-RRB-
cord-002791-tqchfdmy	28	74	.	.	.
cord-002791-tqchfdmy	29	1	While	while	IN
cord-002791-tqchfdmy	29	2	western	western	JJ
cord-002791-tqchfdmy	29	3	blot	blot	NN
cord-002791-tqchfdmy	29	4	analysis	analysis	NN
cord-002791-tqchfdmy	29	5	using	use	VBG
cord-002791-tqchfdmy	29	6	the	the	DT
cord-002791-tqchfdmy	29	7	Anti	Anti	NNP
cord-002791-tqchfdmy	29	8	-	-	NNP
cord-002791-tqchfdmy	29	9	Enterobacterio	Enterobacterio	NNP
cord-002791-tqchfdmy	29	10	Phage	Phage	NNP
cord-002791-tqchfdmy	29	11	MS2	MS2	NNP
cord-002791-tqchfdmy	29	12	Coat	Coat	NNP
cord-002791-tqchfdmy	29	13	Protein	Protein	NNP
cord-002791-tqchfdmy	29	14	polyclonal	polyclonal	JJ
cord-002791-tqchfdmy	29	15	antibody	antibody	NN
cord-002791-tqchfdmy	29	16	identified	identify	VBD
cord-002791-tqchfdmy	29	17	both	both	DT
cord-002791-tqchfdmy	29	18	wild	wild	JJ
cord-002791-tqchfdmy	29	19	-	-	HYPH
cord-002791-tqchfdmy	29	20	type	type	NN
cord-002791-tqchfdmy	29	21	(	(	-LRB-
cord-002791-tqchfdmy	29	22	Fig	Fig	NNP
cord-002791-tqchfdmy	29	23	.	.	.
cord-002791-tqchfdmy	29	24	4,B1	4,b1	CD
cord-002791-tqchfdmy	29	25	)	)	-RRB-
cord-002791-tqchfdmy	29	26	and	and	CC
cord-002791-tqchfdmy	29	27	single	single	JJ
cord-002791-tqchfdmy	29	28	-	-	HYPH
cord-002791-tqchfdmy	29	29	chain	chain	NN
cord-002791-tqchfdmy	29	30	versions	version	NNS
cord-002791-tqchfdmy	29	31	of	of	IN
cord-002791-tqchfdmy	29	32	the	the	DT
cord-002791-tqchfdmy	29	33	coat	coat	NN
cord-002791-tqchfdmy	29	34	protein	protein	NN
cord-002791-tqchfdmy	29	35	dimer	dimer	NN
cord-002791-tqchfdmy	29	36	containing	contain	VBG
cord-002791-tqchfdmy	29	37	the	the	DT
cord-002791-tqchfdmy	29	38	His	-PRON-	PRP$
cord-002791-tqchfdmy	29	39	-	-	HYPH
cord-002791-tqchfdmy	29	40	tag	tag	NN
cord-002791-tqchfdmy	29	41	(	(	-LRB-
cord-002791-tqchfdmy	29	42	Fig	fig	NN
cord-002791-tqchfdmy	29	43	.	.	.
cord-002791-tqchfdmy	29	44	4,B2	4,b2	CD
cord-002791-tqchfdmy	29	45	)	)	-RRB-
cord-002791-tqchfdmy	29	46	,	,	,
cord-002791-tqchfdmy	29	47	the	the	DT
cord-002791-tqchfdmy	29	48	anti	anti	JJ
cord-002791-tqchfdmy	29	49	-	-	JJ
cord-002791-tqchfdmy	29	50	HisTag	histag	JJ
cord-002791-tqchfdmy	29	51	antibody	antibody	NN
cord-002791-tqchfdmy	29	52	specifically	specifically	RB
cord-002791-tqchfdmy	29	53	interacted	interact	VBN
cord-002791-tqchfdmy	29	54	only	only	RB
cord-002791-tqchfdmy	29	55	with	with	IN
cord-002791-tqchfdmy	29	56	the	the	DT
cord-002791-tqchfdmy	29	57	single	single	JJ
cord-002791-tqchfdmy	29	58	-	-	HYPH
cord-002791-tqchfdmy	29	59	chain	chain	NN
cord-002791-tqchfdmy	29	60	version	version	NN
cord-002791-tqchfdmy	29	61	of	of	IN
cord-002791-tqchfdmy	29	62	the	the	DT
cord-002791-tqchfdmy	29	63	coat	coat	NN
cord-002791-tqchfdmy	29	64	protein	protein	NN
cord-002791-tqchfdmy	29	65	dimer	dimer	NN
cord-002791-tqchfdmy	29	66	containing	contain	VBG
cord-002791-tqchfdmy	29	67	the	the	DT
cord-002791-tqchfdmy	29	68	His	-PRON-	PRP$
cord-002791-tqchfdmy	29	69	-	-	HYPH
cord-002791-tqchfdmy	29	70	tag	tag	NN
cord-002791-tqchfdmy	29	71	(	(	-LRB-
cord-002791-tqchfdmy	29	72	Fig	Fig	NNP
cord-002791-tqchfdmy	29	73	.	.	.
cord-002791-tqchfdmy	29	74	4,C2	4,c2	CD
cord-002791-tqchfdmy	29	75	)	)	-RRB-
cord-002791-tqchfdmy	29	76	.	.	.
cord-002791-tqchfdmy	30	1	To	to	TO
cord-002791-tqchfdmy	30	2	calculate	calculate	VB
cord-002791-tqchfdmy	30	3	the	the	DT
cord-002791-tqchfdmy	30	4	exact	exact	JJ
cord-002791-tqchfdmy	30	5	molecular	molecular	JJ
cord-002791-tqchfdmy	30	6	weight	weight	NN
cord-002791-tqchfdmy	30	7	of	of	IN
cord-002791-tqchfdmy	30	8	wild	wild	JJ
cord-002791-tqchfdmy	30	9	-	-	HYPH
cord-002791-tqchfdmy	30	10	type	type	NN
cord-002791-tqchfdmy	30	11	and	and	CC
cord-002791-tqchfdmy	30	12	single	single	JJ
cord-002791-tqchfdmy	30	13	-	-	HYPH
cord-002791-tqchfdmy	30	14	chain	chain	NN
cord-002791-tqchfdmy	30	15	versions	version	NNS
cord-002791-tqchfdmy	30	16	of	of	IN
cord-002791-tqchfdmy	30	17	the	the	DT
cord-002791-tqchfdmy	30	18	coat	coat	NN
cord-002791-tqchfdmy	30	19	protein	protein	NN
cord-002791-tqchfdmy	30	20	dimer	dimer	NN
cord-002791-tqchfdmy	30	21	containing	contain	VBG
cord-002791-tqchfdmy	30	22	the	the	DT
cord-002791-tqchfdmy	30	23	His	-PRON-	PRP$
cord-002791-tqchfdmy	30	24	-	-	HYPH
cord-002791-tqchfdmy	30	25	tag	tag	NN
cord-002791-tqchfdmy	30	26	,	,	,
cord-002791-tqchfdmy	30	27	matrix	matrix	NN
cord-002791-tqchfdmy	30	28	assisted	assist	VBN
cord-002791-tqchfdmy	30	29	laser	laser	NN
cord-002791-tqchfdmy	30	30	desorption	desorption	NN
cord-002791-tqchfdmy	30	31	ionization	ionization	NN
cord-002791-tqchfdmy	30	32	-	-	HYPH
cord-002791-tqchfdmy	30	33	time	time	NN
cord-002791-tqchfdmy	30	34	of	of	IN
cord-002791-tqchfdmy	30	35	flight	flight	NN
cord-002791-tqchfdmy	30	36	mass	mass	NN
cord-002791-tqchfdmy	30	37	spectrometry	spectrometry	NN
cord-002791-tqchfdmy	30	38	(	(	-LRB-
cord-002791-tqchfdmy	30	39	MALDI	MALDI	NNP
cord-002791-tqchfdmy	30	40	-	-	HYPH
cord-002791-tqchfdmy	30	41	TOF	TOF	NNP
cord-002791-tqchfdmy	30	42	)	)	-RRB-
cord-002791-tqchfdmy	30	43	analysis	analysis	NN
cord-002791-tqchfdmy	30	44	was	be	VBD
cord-002791-tqchfdmy	30	45	performed	perform	VBN
cord-002791-tqchfdmy	30	46	.	.	.
cord-002791-tqchfdmy	31	1	The	the	DT
cord-002791-tqchfdmy	31	2	measured	measured	JJ
cord-002791-tqchfdmy	31	3	molecular	molecular	JJ
cord-002791-tqchfdmy	31	4	weight	weight	NN
cord-002791-tqchfdmy	31	5	of	of	IN
cord-002791-tqchfdmy	31	6	the	the	DT
cord-002791-tqchfdmy	31	7	wild	wild	JJ
cord-002791-tqchfdmy	31	8	-	-	HYPH
cord-002791-tqchfdmy	31	9	type	type	NN
cord-002791-tqchfdmy	31	10	coat	coat	NN
cord-002791-tqchfdmy	31	11	protein	protein	NN
cord-002791-tqchfdmy	31	12	was	be	VBD
cord-002791-tqchfdmy	31	13	13,730	13,730	CD
cord-002791-tqchfdmy	31	14	Da	Da	NNP
cord-002791-tqchfdmy	31	15	(	(	-LRB-
cord-002791-tqchfdmy	31	16	Fig	Fig	NNP
cord-002791-tqchfdmy	31	17	.	.	.
cord-002791-tqchfdmy	31	18	4D	4d	CD
cord-002791-tqchfdmy	31	19	)	)	-RRB-
cord-002791-tqchfdmy	31	20	.	.	.
cord-002791-tqchfdmy	32	1	The	the	DT
cord-002791-tqchfdmy	32	2	measured	measured	JJ
cord-002791-tqchfdmy	32	3	molecular	molecular	JJ
cord-002791-tqchfdmy	32	4	weight	weight	NN
cord-002791-tqchfdmy	32	5	of	of	IN
cord-002791-tqchfdmy	32	6	the	the	DT
cord-002791-tqchfdmy	32	7	single	single	JJ
cord-002791-tqchfdmy	32	8	-	-	HYPH
cord-002791-tqchfdmy	32	9	chain	chain	NN
cord-002791-tqchfdmy	32	10	version	version	NN
cord-002791-tqchfdmy	32	11	of	of	IN
cord-002791-tqchfdmy	32	12	the	the	DT
cord-002791-tqchfdmy	32	13	coat	coat	NN
cord-002791-tqchfdmy	32	14	protein	protein	NN
cord-002791-tqchfdmy	32	15	dimer	dimer	NN
cord-002791-tqchfdmy	32	16	containing	contain	VBG
cord-002791-tqchfdmy	32	17	the	the	DT
cord-002791-tqchfdmy	32	18	His	-PRON-	PRP$
cord-002791-tqchfdmy	32	19	-	-	HYPH
cord-002791-tqchfdmy	32	20	tag	tag	NN
cord-002791-tqchfdmy	32	21	was	be	VBD
cord-002791-tqchfdmy	32	22	28,329	28,329	CD
cord-002791-tqchfdmy	32	23	Da	Da	NNP
cord-002791-tqchfdmy	32	24	(	(	-LRB-
cord-002791-tqchfdmy	32	25	Fig	Fig	NNP
cord-002791-tqchfdmy	32	26	.	.	.
cord-002791-tqchfdmy	32	27	4E	4E	NNP
cord-002791-tqchfdmy	32	28	)	)	-RRB-
cord-002791-tqchfdmy	32	29	.	.	.
cord-002791-tqchfdmy	33	1	Testing	testing	NN
cord-002791-tqchfdmy	33	2	of	of	IN
cord-002791-tqchfdmy	33	3	the	the	DT
cord-002791-tqchfdmy	33	4	stability	stability	NN
cord-002791-tqchfdmy	33	5	of	of	IN
cord-002791-tqchfdmy	33	6	His	-PRON-	PRP$
cord-002791-tqchfdmy	33	7	-	-	HYPH
cord-002791-tqchfdmy	33	8	tagged	tag	VBN
cord-002791-tqchfdmy	33	9	MS2	MS2	NNP
cord-002791-tqchfdmy	33	10	PLP	PLP	NNP
cord-002791-tqchfdmy	33	11	against	against	IN
cord-002791-tqchfdmy	33	12	nucleases	nuclease	NNS
cord-002791-tqchfdmy	33	13	showed	show	VBD
cord-002791-tqchfdmy	33	14	them	-PRON-	PRP
cord-002791-tqchfdmy	33	15	to	to	TO
cord-002791-tqchfdmy	33	16	be	be	VB
cord-002791-tqchfdmy	33	17	resistant	resistant	JJ
cord-002791-tqchfdmy	33	18	under	under	IN
cord-002791-tqchfdmy	33	19	conditions	condition	NNS
cord-002791-tqchfdmy	33	20	where	where	WRB
cord-002791-tqchfdmy	33	21	naked	naked	JJ
cord-002791-tqchfdmy	33	22	DNA	DNA	NNP
cord-002791-tqchfdmy	33	23	and/or	and/or	CC
cord-002791-tqchfdmy	33	24	RNA	RNA	NNP
cord-002791-tqchfdmy	33	25	were	be	VBD
cord-002791-tqchfdmy	33	26	rapidly	rapidly	RB
cord-002791-tqchfdmy	33	27	degraded	degrade	VBN
cord-002791-tqchfdmy	33	28	(	(	-LRB-
cord-002791-tqchfdmy	33	29	Fig	fig	NN
cord-002791-tqchfdmy	33	30	.	.	.
cord-002791-tqchfdmy	33	31	5	5	CD
cord-002791-tqchfdmy	33	32	)	)	-RRB-
cord-002791-tqchfdmy	33	33	.	.	.
cord-002791-tqchfdmy	34	1	Schematic	schematic	JJ
cord-002791-tqchfdmy	34	2	representation	representation	NN
cord-002791-tqchfdmy	34	3	of	of	IN
cord-002791-tqchfdmy	34	4	de	de	NNP
cord-002791-tqchfdmy	34	5	novo	novo	NNP
cord-002791-tqchfdmy	34	6	-	-	HYPH
cord-002791-tqchfdmy	34	7	synthesized	synthesize	VBN
cord-002791-tqchfdmy	34	8	sequence	sequence	NN
cord-002791-tqchfdmy	34	9	of	of	IN
cord-002791-tqchfdmy	34	10	maturase	maturase	NN
cord-002791-tqchfdmy	34	11	and	and	CC
cord-002791-tqchfdmy	34	12	single	single	JJ
cord-002791-tqchfdmy	34	13	-	-	HYPH
cord-002791-tqchfdmy	34	14	chain	chain	NN
cord-002791-tqchfdmy	34	15	version	version	NN
cord-002791-tqchfdmy	34	16	of	of	IN
cord-002791-tqchfdmy	34	17	the	the	DT
cord-002791-tqchfdmy	34	18	coat	coat	NN
cord-002791-tqchfdmy	34	19	protein	protein	NN
cord-002791-tqchfdmy	34	20	dimer	dimer	NN
cord-002791-tqchfdmy	34	21	containing	contain	VBG
cord-002791-tqchfdmy	34	22	the	the	DT
cord-002791-tqchfdmy	34	23	His	His	NNP
cord-002791-tqchfdmy	34	24	-	-	HYPH
cord-002791-tqchfdmy	34	25	tag	tag	NN
cord-002791-tqchfdmy	34	26	.	.	.
cord-002791-tqchfdmy	35	1	De	De	NNP
cord-002791-tqchfdmy	35	2	novo	novo	NNP
cord-002791-tqchfdmy	35	3	-	-	HYPH
cord-002791-tqchfdmy	35	4	synthesized	synthesize	VBN
cord-002791-tqchfdmy	35	5	sequence	sequence	NN
cord-002791-tqchfdmy	35	6	was	be	VBD
cord-002791-tqchfdmy	35	7	used	use	VBN
cord-002791-tqchfdmy	35	8	as	as	IN
cord-002791-tqchfdmy	35	9	a	a	DT
cord-002791-tqchfdmy	35	10	PCR	PCR	NNP
cord-002791-tqchfdmy	35	11	template	template	NN
cord-002791-tqchfdmy	35	12	from	from	IN
cord-002791-tqchfdmy	35	13	which	which	WDT
cord-002791-tqchfdmy	35	14	the	the	DT
cord-002791-tqchfdmy	35	15	fragment	fragment	NN
cord-002791-tqchfdmy	35	16	of	of	IN
cord-002791-tqchfdmy	35	17	maturase	maturase	NN
cord-002791-tqchfdmy	35	18	and	and	CC
cord-002791-tqchfdmy	35	19	single	single	JJ
cord-002791-tqchfdmy	35	20	-	-	HYPH
cord-002791-tqchfdmy	35	21	chain	chain	NN
cord-002791-tqchfdmy	35	22	version	version	NN
cord-002791-tqchfdmy	35	23	of	of	IN
cord-002791-tqchfdmy	35	24	the	the	DT
cord-002791-tqchfdmy	35	25	coat	coat	NN
cord-002791-tqchfdmy	35	26	protein	protein	NN
cord-002791-tqchfdmy	35	27	dimer	dimer	NN
cord-002791-tqchfdmy	35	28	containing	contain	VBG
cord-002791-tqchfdmy	35	29	the	the	DT
cord-002791-tqchfdmy	35	30	His	-PRON-	PRP$
cord-002791-tqchfdmy	35	31	-	-	HYPH
cord-002791-tqchfdmy	35	32	tag	tag	NN
cord-002791-tqchfdmy	35	33	was	be	VBD
cord-002791-tqchfdmy	35	34	amplified	amplify	VBN
cord-002791-tqchfdmy	35	35	and	and	CC
cord-002791-tqchfdmy	35	36	subsequently	subsequently	RB
cord-002791-tqchfdmy	35	37	cloned	clone	VBN
cord-002791-tqchfdmy	35	38	to	to	IN
cord-002791-tqchfdmy	35	39	multiple	multiple	JJ
cord-002791-tqchfdmy	35	40	cloning	cloning	NN
cord-002791-tqchfdmy	35	41	site	site	NN
cord-002791-tqchfdmy	35	42	1	1	CD
cord-002791-tqchfdmy	35	43	(	(	-LRB-
cord-002791-tqchfdmy	35	44	MCS1	MCS1	NNP
cord-002791-tqchfdmy	35	45	)	)	-RRB-
cord-002791-tqchfdmy	35	46	of	of	IN
cord-002791-tqchfdmy	35	47	the	the	DT
cord-002791-tqchfdmy	35	48	pACYCDuet-1-TM	pacycduet-1-tm	JJ
cord-002791-tqchfdmy	35	49	.	.	.
cord-002791-tqchfdmy	36	1	The	the	DT
cord-002791-tqchfdmy	36	2	sequence	sequence	NN
cord-002791-tqchfdmy	36	3	in	in	IN
cord-002791-tqchfdmy	36	4	green	green	JJ
cord-002791-tqchfdmy	36	5	is	be	VBZ
cord-002791-tqchfdmy	36	6	the	the	DT
cord-002791-tqchfdmy	36	7	sequence	sequence	NN
cord-002791-tqchfdmy	36	8	of	of	IN
cord-002791-tqchfdmy	36	9	single	single	JJ
cord-002791-tqchfdmy	36	10	-	-	HYPH
cord-002791-tqchfdmy	36	11	chain	chain	NN
cord-002791-tqchfdmy	36	12	coat	coat	NN
cord-002791-tqchfdmy	36	13	protein	protein	NN
cord-002791-tqchfdmy	36	14	dimer	dimer	NN
cord-002791-tqchfdmy	36	15	containing	contain	VBG
cord-002791-tqchfdmy	36	16	His	-PRON-	PRP$
cord-002791-tqchfdmy	36	17	-	-	HYPH
cord-002791-tqchfdmy	36	18	tag	tag	NN
cord-002791-tqchfdmy	36	19	(	(	-LRB-
cord-002791-tqchfdmy	36	20	in	in	IN
cord-002791-tqchfdmy	36	21	blue	blue	JJ
cord-002791-tqchfdmy	36	22	)	)	-RRB-
cord-002791-tqchfdmy	36	23	surrounded	surround	VBN
cord-002791-tqchfdmy	36	24	by	by	IN
cord-002791-tqchfdmy	36	25	two	two	CD
cord-002791-tqchfdmy	36	26	KpnI	KpnI	NNP
cord-002791-tqchfdmy	36	27	restriction	restriction	NN
cord-002791-tqchfdmy	36	28	sites	site	NNS
cord-002791-tqchfdmy	36	29	(	(	-LRB-
cord-002791-tqchfdmy	36	30	in	in	IN
cord-002791-tqchfdmy	36	31	orange	orange	NNP
cord-002791-tqchfdmy	36	32	)	)	-RRB-
cord-002791-tqchfdmy	36	33	which	which	WDT
cord-002791-tqchfdmy	36	34	is	be	VBZ
cord-002791-tqchfdmy	36	35	localized	localize	VBN
cord-002791-tqchfdmy	36	36	between	between	IN
cord-002791-tqchfdmy	36	37	nucleotides	nucleotide	NNS
cord-002791-tqchfdmy	36	38	1255	1255	CD
cord-002791-tqchfdmy	36	39	-	-	SYM
cord-002791-tqchfdmy	36	40	2068	2068	CD
cord-002791-tqchfdmy	36	41	in	in	IN
cord-002791-tqchfdmy	36	42	de	de	NNP
cord-002791-tqchfdmy	36	43	novo	novo	NNP
cord-002791-tqchfdmy	36	44	-	-	HYPH
cord-002791-tqchfdmy	36	45	synthesized	synthesize	VBN
cord-002791-tqchfdmy	36	46	sequence	sequence	NN
cord-002791-tqchfdmy	36	47	.	.	.
cord-002791-tqchfdmy	37	1	Full	full	JJ
cord-002791-tqchfdmy	37	2	length	length	NN
cord-002791-tqchfdmy	37	3	de	de	FW
cord-002791-tqchfdmy	37	4	novo	novo	NN
cord-002791-tqchfdmy	37	5	-	-	HYPH
cord-002791-tqchfdmy	37	6	synthesized	synthesize	VBN
cord-002791-tqchfdmy	37	7	sequence	sequence	NN
cord-002791-tqchfdmy	37	8	is	be	VBZ
cord-002791-tqchfdmy	37	9	presented	present	VBN
cord-002791-tqchfdmy	37	10	in	in	IN
cord-002791-tqchfdmy	37	11	Supplementary	Supplementary	NNP
cord-002791-tqchfdmy	37	12	Figure	Figure	NNP
cord-002791-tqchfdmy	37	13	1	1	CD
cord-002791-tqchfdmy	37	14	.	.	.
cord-002791-tqchfdmy	38	1	,	,	,
cord-002791-tqchfdmy	38	2	molecular	molecular	JJ
cord-002791-tqchfdmy	38	3	weight	weight	NN
cord-002791-tqchfdmy	38	4	of	of	IN
cord-002791-tqchfdmy	38	5	wild	wild	JJ
cord-002791-tqchfdmy	38	6	type	type	NN
cord-002791-tqchfdmy	38	7	MS2	MS2	NNP
cord-002791-tqchfdmy	38	8	bacteriophage	bacteriophage	NN
cord-002791-tqchfdmy	38	9	coat	coat	NN
cord-002791-tqchfdmy	38	10	protein	protein	NN
cord-002791-tqchfdmy	38	11	was	be	VBD
cord-002791-tqchfdmy	38	12	about	about	RB
cord-002791-tqchfdmy	38	13	13	13	CD
cord-002791-tqchfdmy	38	14	kDa	kDa	NNS
cord-002791-tqchfdmy	38	15	(	(	-LRB-
cord-002791-tqchfdmy	38	16	A1	A1	NNP
cord-002791-tqchfdmy	38	17	)	)	-RRB-
cord-002791-tqchfdmy	39	1	whereas	whereas	IN
cord-002791-tqchfdmy	39	2	single	single	JJ
cord-002791-tqchfdmy	39	3	chain	chain	NN
cord-002791-tqchfdmy	39	4	version	version	NN
cord-002791-tqchfdmy	39	5	of	of	IN
cord-002791-tqchfdmy	39	6	the	the	DT
cord-002791-tqchfdmy	39	7	MS2	MS2	NNP
cord-002791-tqchfdmy	39	8	coat	coat	NN
cord-002791-tqchfdmy	39	9	protein	protein	NN
cord-002791-tqchfdmy	39	10	dimer	dimer	NNP
cord-002791-tqchfdmy	39	11	containing	contain	VBG
cord-002791-tqchfdmy	39	12	the	the	DT
cord-002791-tqchfdmy	39	13	Histag	Histag	NNP
cord-002791-tqchfdmy	39	14	was	be	VBD
cord-002791-tqchfdmy	39	15	about	about	RB
cord-002791-tqchfdmy	39	16	28	28	CD
cord-002791-tqchfdmy	39	17	kDa	kDa	NNPS
cord-002791-tqchfdmy	39	18	(	(	-LRB-
cord-002791-tqchfdmy	39	19	A2	A2	NNP
cord-002791-tqchfdmy	39	20	)	)	-RRB-
cord-002791-tqchfdmy	39	21	;	;	:
cord-002791-tqchfdmy	39	22	(	(	-LRB-
cord-002791-tqchfdmy	39	23	B	b	NN
cord-002791-tqchfdmy	39	24	)	)	-RRB-
cord-002791-tqchfdmy	39	25	western	western	JJ
cord-002791-tqchfdmy	39	26	blot	blot	NN
cord-002791-tqchfdmy	39	27	analysis	analysis	NN
cord-002791-tqchfdmy	39	28	using	use	VBG
cord-002791-tqchfdmy	39	29	primary	primary	JJ
cord-002791-tqchfdmy	39	30	Anti	Anti	NNP
cord-002791-tqchfdmy	39	31	-	-	NNP
cord-002791-tqchfdmy	39	32	Enterobacterio	Enterobacterio	NNP
cord-002791-tqchfdmy	39	33	Phage	Phage	NNP
cord-002791-tqchfdmy	39	34	MS2	MS2	NNP
cord-002791-tqchfdmy	39	35	Coat	Coat	NNP
cord-002791-tqchfdmy	39	36	Protein	Protein	NNP
cord-002791-tqchfdmy	39	37	polyclonal	polyclonal	JJ
cord-002791-tqchfdmy	39	38	antibody	antibody	NN
cord-002791-tqchfdmy	40	1	(	(	-LRB-
cord-002791-tqchfdmy	40	2	Merck	Merck	NNP
cord-002791-tqchfdmy	40	3	Millipore	Millipore	NNP
cord-002791-tqchfdmy	40	4	,	,	,
cord-002791-tqchfdmy	40	5	USA	USA	NNP
cord-002791-tqchfdmy	40	6	)	)	-RRB-
cord-002791-tqchfdmy	40	7	and	and	CC
cord-002791-tqchfdmy	40	8	secondary	secondary	JJ
cord-002791-tqchfdmy	40	9	goat	goat	NN
cord-002791-tqchfdmy	40	10	anti	anti	JJ
cord-002791-tqchfdmy	40	11	-	-	JJ
cord-002791-tqchfdmy	40	12	rabbit	rabbit	JJ
cord-002791-tqchfdmy	40	13	IgG	igg	NN
cord-002791-tqchfdmy	40	14	conjugated	conjugate	VBN
cord-002791-tqchfdmy	40	15	with	with	IN
cord-002791-tqchfdmy	40	16	HRP	HRP	NNP
cord-002791-tqchfdmy	40	17	(	(	-LRB-
cord-002791-tqchfdmy	40	18	Jackson	Jackson	NNP
cord-002791-tqchfdmy	40	19	ImmunoResearch	ImmunoResearch	NNP
cord-002791-tqchfdmy	40	20	,	,	,
cord-002791-tqchfdmy	40	21	UK	UK	NNP
cord-002791-tqchfdmy	40	22	)	)	-RRB-
cord-002791-tqchfdmy	40	23	antibodies	antibody	NNS
cord-002791-tqchfdmy	40	24	at	at	IN
cord-002791-tqchfdmy	40	25	wild	wild	JJ
cord-002791-tqchfdmy	40	26	type	type	NN
cord-002791-tqchfdmy	40	27	MS2	MS2	NNP
cord-002791-tqchfdmy	40	28	bacteriophage	bacteriophage	NN
cord-002791-tqchfdmy	40	29	coat	coat	NN
cord-002791-tqchfdmy	40	30	protein	protein	NN
cord-002791-tqchfdmy	40	31	(	(	-LRB-
cord-002791-tqchfdmy	40	32	B1	b1	NN
cord-002791-tqchfdmy	40	33	)	)	-RRB-
cord-002791-tqchfdmy	40	34	and	and	CC
cord-002791-tqchfdmy	40	35	single	single	JJ
cord-002791-tqchfdmy	40	36	chain	chain	NN
cord-002791-tqchfdmy	40	37	version	version	NN
cord-002791-tqchfdmy	40	38	of	of	IN
cord-002791-tqchfdmy	40	39	the	the	DT
cord-002791-tqchfdmy	40	40	MS2	MS2	NNP
cord-002791-tqchfdmy	40	41	coat	coat	NN
cord-002791-tqchfdmy	40	42	protein	protein	NN
cord-002791-tqchfdmy	40	43	dimer	dimer	NNP
cord-002791-tqchfdmy	40	44	containing	contain	VBG
cord-002791-tqchfdmy	40	45	the	the	DT
cord-002791-tqchfdmy	40	46	His	-PRON-	PRP$
cord-002791-tqchfdmy	40	47	-	-	HYPH
cord-002791-tqchfdmy	40	48	tag	tag	NN
cord-002791-tqchfdmy	40	49	(	(	-LRB-
cord-002791-tqchfdmy	40	50	B2	b2	NN
cord-002791-tqchfdmy	40	51	)	)	-RRB-
cord-002791-tqchfdmy	40	52	;	;	:
cord-002791-tqchfdmy	40	53	(	(	-LRB-
cord-002791-tqchfdmy	40	54	C	C	NNP
cord-002791-tqchfdmy	40	55	)	)	-RRB-
cord-002791-tqchfdmy	40	56	western	western	JJ
cord-002791-tqchfdmy	40	57	blot	blot	NN
cord-002791-tqchfdmy	40	58	analysis	analysis	NN
cord-002791-tqchfdmy	40	59	using	use	VBG
cord-002791-tqchfdmy	40	60	primary	primary	JJ
cord-002791-tqchfdmy	40	61	anti	anti	JJ
cord-002791-tqchfdmy	40	62	-	-	JJ
cord-002791-tqchfdmy	40	63	HisTag	histag	JJ
cord-002791-tqchfdmy	40	64	antibody	antibody	NN
cord-002791-tqchfdmy	40	65	(	(	-LRB-
cord-002791-tqchfdmy	40	66	Pierce	Pierce	NNP
cord-002791-tqchfdmy	40	67	,	,	,
cord-002791-tqchfdmy	40	68	Thermo	Thermo	NNP
cord-002791-tqchfdmy	40	69	Scientific	Scientific	NNP
cord-002791-tqchfdmy	40	70	,	,	,
cord-002791-tqchfdmy	40	71	USA	USA	NNP
cord-002791-tqchfdmy	40	72	)	)	-RRB-
cord-002791-tqchfdmy	40	73	and	and	CC
cord-002791-tqchfdmy	40	74	secondary	secondary	JJ
cord-002791-tqchfdmy	40	75	goat	goat	NN
cord-002791-tqchfdmy	40	76	anti	anti	JJ
cord-002791-tqchfdmy	40	77	-	-	JJ
cord-002791-tqchfdmy	40	78	mouse	mouse	JJ
cord-002791-tqchfdmy	40	79	IgG	IgG	NNP
cord-002791-tqchfdmy	40	80	conjugated	conjugate	VBN
cord-002791-tqchfdmy	40	81	with	with	IN
cord-002791-tqchfdmy	40	82	HRP	HRP	NNP
cord-002791-tqchfdmy	40	83	(	(	-LRB-
cord-002791-tqchfdmy	40	84	Jackson	Jackson	NNP
cord-002791-tqchfdmy	40	85	ImmunoResearch	ImmunoResearch	NNP
cord-002791-tqchfdmy	40	86	)	)	-RRB-
cord-002791-tqchfdmy	40	87	antibodies	antibody	NNS
cord-002791-tqchfdmy	40	88	at	at	IN
cord-002791-tqchfdmy	40	89	wild	wild	JJ
cord-002791-tqchfdmy	40	90	type	type	NN
cord-002791-tqchfdmy	40	91	MS2	MS2	NNP
cord-002791-tqchfdmy	40	92	bacteriophage	bacteriophage	NN
cord-002791-tqchfdmy	40	93	coat	coat	NN
cord-002791-tqchfdmy	40	94	protein	protein	NN
cord-002791-tqchfdmy	40	95	(	(	-LRB-
cord-002791-tqchfdmy	40	96	C1	C1	NNP
cord-002791-tqchfdmy	40	97	)	)	-RRB-
cord-002791-tqchfdmy	40	98	and	and	CC
cord-002791-tqchfdmy	40	99	single	single	JJ
cord-002791-tqchfdmy	40	100	chain	chain	NN
cord-002791-tqchfdmy	40	101	version	version	NN
cord-002791-tqchfdmy	40	102	of	of	IN
cord-002791-tqchfdmy	40	103	the	the	DT
cord-002791-tqchfdmy	40	104	MS2	MS2	NNP
cord-002791-tqchfdmy	40	105	coat	coat	NN
cord-002791-tqchfdmy	40	106	protein	protein	NN
cord-002791-tqchfdmy	40	107	dimer	dimer	NNP
cord-002791-tqchfdmy	40	108	containing	contain	VBG
cord-002791-tqchfdmy	40	109	the	the	DT
cord-002791-tqchfdmy	40	110	His	-PRON-	PRP$
cord-002791-tqchfdmy	40	111	-	-	HYPH
cord-002791-tqchfdmy	40	112	tag	tag	NN
cord-002791-tqchfdmy	40	113	(	(	-LRB-
cord-002791-tqchfdmy	40	114	C2	C2	NNP
cord-002791-tqchfdmy	40	115	)	)	-RRB-
cord-002791-tqchfdmy	40	116	;	;	:
cord-002791-tqchfdmy	40	117	(	(	-LRB-
cord-002791-tqchfdmy	40	118	D	D	NNP
cord-002791-tqchfdmy	40	119	,	,	,
cord-002791-tqchfdmy	40	120	E	e	NN
cord-002791-tqchfdmy	40	121	)	)	-RRB-
cord-002791-tqchfdmy	40	122	laser	laser	NN
cord-002791-tqchfdmy	40	123	desorption	desorption	NN
cord-002791-tqchfdmy	40	124	ionization	ionization	NN
cord-002791-tqchfdmy	40	125	-	-	HYPH
cord-002791-tqchfdmy	40	126	time	time	NN
cord-002791-tqchfdmy	40	127	of	of	IN
cord-002791-tqchfdmy	40	128	flight	flight	NN
cord-002791-tqchfdmy	40	129	mass	mass	NN
cord-002791-tqchfdmy	40	130	spectrometry	spectrometry	NN
cord-002791-tqchfdmy	40	131	(	(	-LRB-
cord-002791-tqchfdmy	40	132	MALDI	MALDI	NNP
cord-002791-tqchfdmy	40	133	-	-	HYPH
cord-002791-tqchfdmy	40	134	TOF	TOF	NNP
cord-002791-tqchfdmy	40	135	)	)	-RRB-
cord-002791-tqchfdmy	40	136	analysis	analysis	NN
cord-002791-tqchfdmy	40	137	of	of	IN
cord-002791-tqchfdmy	40	138	wild	wild	JJ
cord-002791-tqchfdmy	40	139	-	-	HYPH
cord-002791-tqchfdmy	40	140	type	type	NN
cord-002791-tqchfdmy	40	141	bacteriophage	bacteriophage	NN
cord-002791-tqchfdmy	40	142	MS2	ms2	NN
cord-002791-tqchfdmy	40	143	coat	coat	NN
cord-002791-tqchfdmy	40	144	protein	protein	NN
cord-002791-tqchfdmy	40	145	and	and	CC
cord-002791-tqchfdmy	40	146	single	single	JJ
cord-002791-tqchfdmy	40	147	-	-	HYPH
cord-002791-tqchfdmy	40	148	chain	chain	NN
cord-002791-tqchfdmy	40	149	version	version	NN
cord-002791-tqchfdmy	40	150	of	of	IN
cord-002791-tqchfdmy	40	151	the	the	DT
cord-002791-tqchfdmy	40	152	MS2	MS2	NNP
cord-002791-tqchfdmy	40	153	coat	coat	NN
cord-002791-tqchfdmy	40	154	protein	protein	NN
cord-002791-tqchfdmy	40	155	dimer	dimer	NNP
cord-002791-tqchfdmy	40	156	containing	contain	VBG
cord-002791-tqchfdmy	40	157	the	the	DT
cord-002791-tqchfdmy	40	158	His	-PRON-	PRP$
cord-002791-tqchfdmy	40	159	-	-	HYPH
cord-002791-tqchfdmy	40	160	tag	tag	NN
cord-002791-tqchfdmy	40	161	;	;	:
cord-002791-tqchfdmy	40	162	M	M	NNP
cord-002791-tqchfdmy	40	163	,	,	,
cord-002791-tqchfdmy	40	164	marker	marker	NN
cord-002791-tqchfdmy	40	165	,	,	,
cord-002791-tqchfdmy	40	166	spectra	spectra	JJ
cord-002791-tqchfdmy	40	167	multicolor	multicolor	JJ
cord-002791-tqchfdmy	40	168	broad	broad	JJ
cord-002791-tqchfdmy	40	169	range	range	NN
cord-002791-tqchfdmy	40	170	protein	protein	NN
cord-002791-tqchfdmy	40	171	ladder	ladder	NN
cord-002791-tqchfdmy	40	172	(	(	-LRB-
cord-002791-tqchfdmy	40	173	Fermentas	Fermentas	NNP
cord-002791-tqchfdmy	40	174	)	)	-RRB-
cord-002791-tqchfdmy	40	175	;	;	:
cord-002791-tqchfdmy	40	176	the	the	DT
cord-002791-tqchfdmy	40	177	marker	marker	NN
cord-002791-tqchfdmy	40	178	values	value	NNS
cord-002791-tqchfdmy	40	179	are	be	VBP
cord-002791-tqchfdmy	40	180	in	in	IN
cord-002791-tqchfdmy	40	181	kDa	kDa	NNP
cord-002791-tqchfdmy	40	182	.	.	.
cord-002791-tqchfdmy	41	1	The	the	DT
cord-002791-tqchfdmy	41	2	figures	figure	NNS
cord-002791-tqchfdmy	41	3	were	be	VBD
cord-002791-tqchfdmy	41	4	cropped	crop	VBN
cord-002791-tqchfdmy	41	5	,	,	,
cord-002791-tqchfdmy	41	6	full	full	JJ
cord-002791-tqchfdmy	41	7	length	length	NN
cord-002791-tqchfdmy	41	8	gel	gel	NN
cord-002791-tqchfdmy	41	9	and	and	CC
cord-002791-tqchfdmy	41	10	blots	blot	NNS
cord-002791-tqchfdmy	41	11	are	be	VBP
cord-002791-tqchfdmy	41	12	presented	present	VBN
cord-002791-tqchfdmy	41	13	in	in	IN
cord-002791-tqchfdmy	41	14	Supplementary	Supplementary	NNP
cord-002791-tqchfdmy	41	15	Figures	figure	NNS
cord-002791-tqchfdmy	41	16	2	2	CD
cord-002791-tqchfdmy	41	17	,	,	,
cord-002791-tqchfdmy	41	18	3	3	CD
cord-002791-tqchfdmy	41	19	Determination	determination	NN
cord-002791-tqchfdmy	41	20	of	of	IN
cord-002791-tqchfdmy	41	21	optimal	optimal	JJ
cord-002791-tqchfdmy	41	22	method	method	NN
cord-002791-tqchfdmy	41	23	of	of	IN
cord-002791-tqchfdmy	41	24	thermal	thermal	JJ
cord-002791-tqchfdmy	41	25	lysis	lysis	NN
cord-002791-tqchfdmy	41	26	of	of	IN
cord-002791-tqchfdmy	41	27	His	-PRON-	PRP$
cord-002791-tqchfdmy	41	28	-	-	HYPH
cord-002791-tqchfdmy	41	29	tagged	tag	VBN
cord-002791-tqchfdmy	41	30	MS2	MS2	NNP
cord-002791-tqchfdmy	41	31	PLP	PLP	NNP
cord-002791-tqchfdmy	41	32	and	and	CC
cord-002791-tqchfdmy	41	33	comparison	comparison	NN
cord-002791-tqchfdmy	41	34	of	of	IN
cord-002791-tqchfdmy	41	35	quantity	quantity	NN
cord-002791-tqchfdmy	41	36	and	and	CC
cord-002791-tqchfdmy	41	37	purity	purity	NN
cord-002791-tqchfdmy	41	38	of	of	IN
cord-002791-tqchfdmy	41	39	MS2	MS2	NNP
cord-002791-tqchfdmy	41	40	PLP	PLP	NNP
cord-002791-tqchfdmy	41	41	and	and	CC
cord-002791-tqchfdmy	41	42	His	-PRON-	PRP$
cord-002791-tqchfdmy	41	43	-	-	HYPH
cord-002791-tqchfdmy	41	44	tagged	tag	VBN
cord-002791-tqchfdmy	41	45	MS2	MS2	NNP
cord-002791-tqchfdmy	41	46	PLP	PLP	NNP
cord-002791-tqchfdmy	41	47	.	.	.
cord-002791-tqchfdmy	42	1	The	the	DT
cord-002791-tqchfdmy	42	2	RT	RT	NNP
cord-002791-tqchfdmy	42	3	-	-	HYPH
cord-002791-tqchfdmy	42	4	qPCR	qpcr	NN
cord-002791-tqchfdmy	42	5	results	result	NNS
cord-002791-tqchfdmy	42	6	showing	show	VBG
cord-002791-tqchfdmy	42	7	the	the	DT
cord-002791-tqchfdmy	42	8	most	most	RBS
cord-002791-tqchfdmy	42	9	suitable	suitable	JJ
cord-002791-tqchfdmy	42	10	conditions	condition	NNS
cord-002791-tqchfdmy	42	11	for	for	IN
cord-002791-tqchfdmy	42	12	thermal	thermal	JJ
cord-002791-tqchfdmy	42	13	lysis	lysis	NN
cord-002791-tqchfdmy	42	14	of	of	IN
cord-002791-tqchfdmy	42	15	His	-PRON-	PRP$
cord-002791-tqchfdmy	42	16	-	-	HYPH
cord-002791-tqchfdmy	42	17	tagged	tag	VBN
cord-002791-tqchfdmy	42	18	MS2	MS2	NNP
cord-002791-tqchfdmy	42	19	PLP	PLP	NNP
cord-002791-tqchfdmy	42	20	are	be	VBP
cord-002791-tqchfdmy	42	21	summarized	summarize	VBN
cord-002791-tqchfdmy	42	22	in	in	IN
cord-002791-tqchfdmy	42	23	Table	Table	NNP
cord-002791-tqchfdmy	42	24	1	1	CD
cord-002791-tqchfdmy	42	25	(	(	-LRB-
cord-002791-tqchfdmy	42	26	Table	table	NN
cord-002791-tqchfdmy	42	27	1	1	CD
cord-002791-tqchfdmy	42	28	)	)	-RRB-
cord-002791-tqchfdmy	42	29	.	.	.
cord-002791-tqchfdmy	43	1	Based	base	VBN
cord-002791-tqchfdmy	43	2	on	on	IN
cord-002791-tqchfdmy	43	3	these	these	DT
cord-002791-tqchfdmy	43	4	results	result	NNS
cord-002791-tqchfdmy	43	5	,	,	,
cord-002791-tqchfdmy	43	6	it	-PRON-	PRP
cord-002791-tqchfdmy	43	7	was	be	VBD
cord-002791-tqchfdmy	43	8	decided	decide	VBN
cord-002791-tqchfdmy	43	9	to	to	TO
cord-002791-tqchfdmy	43	10	include	include	VB
cord-002791-tqchfdmy	43	11	the	the	DT
cord-002791-tqchfdmy	43	12	step	step	NN
cord-002791-tqchfdmy	43	13	of	of	IN
cord-002791-tqchfdmy	43	14	particle	particle	NN
cord-002791-tqchfdmy	43	15	lysis	lysis	NN
cord-002791-tqchfdmy	43	16	into	into	IN
cord-002791-tqchfdmy	43	17	the	the	DT
cord-002791-tqchfdmy	43	18	step	step	NN
cord-002791-tqchfdmy	43	19	of	of	IN
cord-002791-tqchfdmy	43	20	initial	initial	JJ
cord-002791-tqchfdmy	43	21	denaturation	denaturation	NN
cord-002791-tqchfdmy	43	22	in	in	IN
cord-002791-tqchfdmy	43	23	RT	RT	NNP
cord-002791-tqchfdmy	43	24	protocol	protocol	NN
cord-002791-tqchfdmy	43	25	.	.	.
cord-002791-tqchfdmy	44	1	The	the	DT
cord-002791-tqchfdmy	44	2	RT	RT	NNP
cord-002791-tqchfdmy	44	3	-	-	HYPH
cord-002791-tqchfdmy	44	4	qPCR	qpcr	NN
cord-002791-tqchfdmy	44	5	results	result	NNS
cord-002791-tqchfdmy	44	6	comparing	compare	VBG
cord-002791-tqchfdmy	44	7	the	the	DT
cord-002791-tqchfdmy	44	8	quantity	quantity	NN
cord-002791-tqchfdmy	44	9	and	and	CC
cord-002791-tqchfdmy	44	10	purity	purity	NN
cord-002791-tqchfdmy	44	11	of	of	IN
cord-002791-tqchfdmy	44	12	MS2	MS2	NNP
cord-002791-tqchfdmy	44	13	PLP	PLP	NNP
cord-002791-tqchfdmy	44	14	and	and	CC
cord-002791-tqchfdmy	44	15	His	-PRON-	PRP$
cord-002791-tqchfdmy	44	16	-	-	HYPH
cord-002791-tqchfdmy	44	17	tagged	tag	VBN
cord-002791-tqchfdmy	44	18	MS2	MS2	NNP
cord-002791-tqchfdmy	44	19	PLP	PLP	NNP
cord-002791-tqchfdmy	44	20	are	be	VBP
cord-002791-tqchfdmy	44	21	summarized	summarize	VBN
cord-002791-tqchfdmy	44	22	in	in	IN
cord-002791-tqchfdmy	44	23	Table	Table	NNP
cord-002791-tqchfdmy	44	24	2	2	CD
cord-002791-tqchfdmy	44	25	(	(	-LRB-
cord-002791-tqchfdmy	44	26	Table	table	NN
cord-002791-tqchfdmy	44	27	2	2	CD
cord-002791-tqchfdmy	44	28	)	)	-RRB-
cord-002791-tqchfdmy	44	29	.	.	.
cord-002791-tqchfdmy	45	1	It	-PRON-	PRP
cord-002791-tqchfdmy	45	2	was	be	VBD
cord-002791-tqchfdmy	45	3	shown	show	VBN
cord-002791-tqchfdmy	45	4	previously	previously	RB
cord-002791-tqchfdmy	45	5	that	that	IN
cord-002791-tqchfdmy	45	6	the	the	DT
cord-002791-tqchfdmy	45	7	newest	new	JJS
cord-002791-tqchfdmy	45	8	and	and	CC
cord-002791-tqchfdmy	45	9	most	most	RBS
cord-002791-tqchfdmy	45	10	advanced	advanced	JJ
cord-002791-tqchfdmy	45	11	in	in	IN
cord-002791-tqchfdmy	45	12	vivo	vivo	NN
cord-002791-tqchfdmy	45	13	system	system	NN
cord-002791-tqchfdmy	45	14	for	for	IN
cord-002791-tqchfdmy	45	15	production	production	NN
cord-002791-tqchfdmy	45	16	of	of	IN
cord-002791-tqchfdmy	45	17	MS2	MS2	NNP
cord-002791-tqchfdmy	45	18	PLP	PLP	NNP
cord-002791-tqchfdmy	45	19	-	-	:
cord-002791-tqchfdmy	45	20	the	the	DT
cord-002791-tqchfdmy	45	21	one	one	CD
cord-002791-tqchfdmy	45	22	-	-	HYPH
cord-002791-tqchfdmy	45	23	plasmid	plasmid	NN
cord-002791-tqchfdmy	45	24	double	double	JJ
cord-002791-tqchfdmy	45	25	-	-	HYPH
cord-002791-tqchfdmy	45	26	expression	expression	NN
cord-002791-tqchfdmy	45	27	system	system	NN
cord-002791-tqchfdmy	45	28	-	-	:
cord-002791-tqchfdmy	45	29	has	have	VBZ
cord-002791-tqchfdmy	45	30	great	great	JJ
cord-002791-tqchfdmy	45	31	potential	potential	NN
cord-002791-tqchfdmy	45	32	for	for	IN
cord-002791-tqchfdmy	45	33	the	the	DT
cord-002791-tqchfdmy	45	34	production	production	NN
cord-002791-tqchfdmy	45	35	of	of	IN
cord-002791-tqchfdmy	45	36	MS2	MS2	NNP
cord-002791-tqchfdmy	45	37	PLP	PLP	NNP
cord-002791-tqchfdmy	45	38	11	11	CD
cord-002791-tqchfdmy	45	39	,	,	,
cord-002791-tqchfdmy	45	40	12	12	CD
cord-002791-tqchfdmy	45	41	.	.	.
cord-002791-tqchfdmy	46	1	In	in	IN
cord-002791-tqchfdmy	46	2	comparison	comparison	NN
cord-002791-tqchfdmy	46	3	with	with	IN
cord-002791-tqchfdmy	46	4	other	other	JJ
cord-002791-tqchfdmy	46	5	previously	previously	RB
cord-002791-tqchfdmy	46	6	used	use	VBN
cord-002791-tqchfdmy	46	7	systems	system	NNS
cord-002791-tqchfdmy	46	8	,	,	,
cord-002791-tqchfdmy	46	9	the	the	DT
cord-002791-tqchfdmy	46	10	system	system	NN
cord-002791-tqchfdmy	46	11	developed	develop	VBN
cord-002791-tqchfdmy	46	12	by	by	IN
cord-002791-tqchfdmy	46	13	Zhan	Zhan	NNP
cord-002791-tqchfdmy	46	14	et	et	NNP
cord-002791-tqchfdmy	46	15	al	al	NNP
cord-002791-tqchfdmy	46	16	.	.	NNP
cord-002791-tqchfdmy	46	17	has	have	VBZ
cord-002791-tqchfdmy	46	18	a	a	DT
cord-002791-tqchfdmy	46	19	good	good	JJ
cord-002791-tqchfdmy	46	20	expression	expression	NN
cord-002791-tqchfdmy	46	21	efficiency	efficiency	NN
cord-002791-tqchfdmy	46	22	,	,	,
cord-002791-tqchfdmy	46	23	produces	produce	VBZ
cord-002791-tqchfdmy	46	24	MS2	MS2	NNP
cord-002791-tqchfdmy	46	25	PLP	PLP	NNP
cord-002791-tqchfdmy	46	26	containing	contain	VBG
cord-002791-tqchfdmy	46	27	only	only	RB
cord-002791-tqchfdmy	46	28	defined	define	VBN
cord-002791-tqchfdmy	46	29	control	control	NN
cord-002791-tqchfdmy	46	30	ssRNA	ssrna	NN
cord-002791-tqchfdmy	46	31	sequences	sequence	NNS
cord-002791-tqchfdmy	46	32	without	without	IN
cord-002791-tqchfdmy	46	33	any	any	DT
cord-002791-tqchfdmy	46	34	other	other	JJ
cord-002791-tqchfdmy	46	35	MS2	MS2	NNP
cord-002791-tqchfdmy	46	36	bacteriophage	bacteriophage	NN
cord-002791-tqchfdmy	46	37	sequences	sequence	NNS
cord-002791-tqchfdmy	46	38	,	,	,
cord-002791-tqchfdmy	46	39	and	and	CC
cord-002791-tqchfdmy	46	40	also	also	RB
cord-002791-tqchfdmy	46	41	allows	allow	VBZ
cord-002791-tqchfdmy	46	42	packaging	packaging	NN
cord-002791-tqchfdmy	46	43	of	of	IN
cord-002791-tqchfdmy	46	44	long	long	JJ
cord-002791-tqchfdmy	46	45	control	control	NN
cord-002791-tqchfdmy	46	46	ssRNA	ssrna	NN
cord-002791-tqchfdmy	46	47	sequences	sequence	NNS
cord-002791-tqchfdmy	46	48	using	use	VBG
cord-002791-tqchfdmy	46	49	multiple	multiple	JJ
cord-002791-tqchfdmy	46	50	pac	pac	NNP
cord-002791-tqchfdmy	46	51	sites	site	NNS
cord-002791-tqchfdmy	46	52	[	[	-LRB-
cord-002791-tqchfdmy	46	53	10	10	CD
cord-002791-tqchfdmy	46	54	]	]	-RRB-
cord-002791-tqchfdmy	46	55	[	[	-LRB-
cord-002791-tqchfdmy	46	56	11	11	CD
cord-002791-tqchfdmy	46	57	]	]	-RRB-
cord-002791-tqchfdmy	46	58	[	[	-LRB-
cord-002791-tqchfdmy	46	59	12	12	CD
cord-002791-tqchfdmy	46	60	]	]	-RRB-
cord-002791-tqchfdmy	46	61	.	.	.
cord-002791-tqchfdmy	47	1	However	however	RB
cord-002791-tqchfdmy	47	2	,	,	,
cord-002791-tqchfdmy	47	3	the	the	DT
cord-002791-tqchfdmy	47	4	one	one	CD
cord-002791-tqchfdmy	47	5	-	-	HYPH
cord-002791-tqchfdmy	47	6	plasmid	plasmid	NN
cord-002791-tqchfdmy	47	7	double	double	JJ
cord-002791-tqchfdmy	47	8	-	-	HYPH
cord-002791-tqchfdmy	47	9	expression	expression	NN
cord-002791-tqchfdmy	47	10	system	system	NN
cord-002791-tqchfdmy	47	11	does	do	VBZ
cord-002791-tqchfdmy	47	12	not	not	RB
cord-002791-tqchfdmy	47	13	enable	enable	VB
cord-002791-tqchfdmy	47	14	rapid	rapid	JJ
cord-002791-tqchfdmy	47	15	,	,	,
cord-002791-tqchfdmy	47	16	simple	simple	JJ
cord-002791-tqchfdmy	47	17	,	,	,
cord-002791-tqchfdmy	47	18	and	and	CC
cord-002791-tqchfdmy	47	19	effective	effective	JJ
cord-002791-tqchfdmy	47	20	purification	purification	NN
cord-002791-tqchfdmy	47	21	of	of	IN
cord-002791-tqchfdmy	47	22	produced	produce	VBN
cord-002791-tqchfdmy	47	23	MS2	MS2	NNP
cord-002791-tqchfdmy	47	24	PLP	PLP	NNP
cord-002791-tqchfdmy	47	25	.	.	.
cord-002791-tqchfdmy	48	1	This	this	DT
cord-002791-tqchfdmy	48	2	problem	problem	NN
cord-002791-tqchfdmy	48	3	can	can	MD
cord-002791-tqchfdmy	48	4	be	be	VB
cord-002791-tqchfdmy	48	5	resolved	resolve	VBN
cord-002791-tqchfdmy	48	6	by	by	IN
cord-002791-tqchfdmy	48	7	introduction	introduction	NN
cord-002791-tqchfdmy	48	8	of	of	IN
cord-002791-tqchfdmy	48	9	a	a	DT
cord-002791-tqchfdmy	48	10	His	-PRON-	PRP$
cord-002791-tqchfdmy	48	11	-	-	HYPH
cord-002791-tqchfdmy	48	12	tag	tag	NN
cord-002791-tqchfdmy	48	13	into	into	IN
cord-002791-tqchfdmy	48	14	the	the	DT
cord-002791-tqchfdmy	48	15	structure	structure	NN
cord-002791-tqchfdmy	48	16	of	of	IN
cord-002791-tqchfdmy	48	17	MS2	MS2	NNP
cord-002791-tqchfdmy	48	18	PLP	PLP	NNP
cord-002791-tqchfdmy	48	19	.	.	.
cord-002791-tqchfdmy	49	1	This	this	DT
cord-002791-tqchfdmy	49	2	results	result	VBZ
cord-002791-tqchfdmy	49	3	in	in	IN
cord-002791-tqchfdmy	49	4	MS2	MS2	NNP
cord-002791-tqchfdmy	49	5	PLP	PLP	NNP
cord-002791-tqchfdmy	49	6	carrying	carry	VBG
cord-002791-tqchfdmy	49	7	180	180	CD
cord-002791-tqchfdmy	49	8	His	-PRON-	PRP$
cord-002791-tqchfdmy	49	9	-	-	HYPH
cord-002791-tqchfdmy	49	10	tag	tag	NN
cord-002791-tqchfdmy	49	11	epitopes	epitope	NNS
cord-002791-tqchfdmy	49	12	,	,	,
cord-002791-tqchfdmy	49	13	which	which	WDT
cord-002791-tqchfdmy	49	14	allows	allow	VBZ
cord-002791-tqchfdmy	49	15	their	-PRON-	PRP$
cord-002791-tqchfdmy	49	16	purification	purification	NN
cord-002791-tqchfdmy	49	17	using	use	VBG
cord-002791-tqchfdmy	49	18	affinity	affinity	NN
cord-002791-tqchfdmy	49	19	chromatography	chromatography	NN
cord-002791-tqchfdmy	49	20	13	13	CD
cord-002791-tqchfdmy	49	21	.	.	.
cord-002791-tqchfdmy	50	1	Unfortunately	unfortunately	RB
cord-002791-tqchfdmy	50	2	,	,	,
cord-002791-tqchfdmy	50	3	at	at	IN
cord-002791-tqchfdmy	50	4	the	the	DT
cord-002791-tqchfdmy	50	5	beginning	beginning	NN
cord-002791-tqchfdmy	50	6	of	of	IN
cord-002791-tqchfdmy	50	7	this	this	DT
cord-002791-tqchfdmy	50	8	study	study	NN
cord-002791-tqchfdmy	50	9	it	-PRON-	PRP
cord-002791-tqchfdmy	50	10	was	be	VBD
cord-002791-tqchfdmy	50	11	found	find	VBN
cord-002791-tqchfdmy	50	12	that	that	IN
cord-002791-tqchfdmy	50	13	introduction	introduction	NN
cord-002791-tqchfdmy	50	14	of	of	IN
cord-002791-tqchfdmy	50	15	a	a	DT
cord-002791-tqchfdmy	50	16	His	-PRON-	PRP$
cord-002791-tqchfdmy	50	17	-	-	HYPH
cord-002791-tqchfdmy	50	18	tag	tag	NN
cord-002791-tqchfdmy	50	19	into	into	IN
cord-002791-tqchfdmy	50	20	the	the	DT
cord-002791-tqchfdmy	50	21	structure	structure	NN
cord-002791-tqchfdmy	50	22	of	of	IN
cord-002791-tqchfdmy	50	23	MS2	MS2	NNP
cord-002791-tqchfdmy	50	24	PLP	PLP	NNP
cord-002791-tqchfdmy	50	25	according	accord	VBG
cord-002791-tqchfdmy	50	26	to	to	IN
cord-002791-tqchfdmy	50	27	Cheng	Cheng	NNP
cord-002791-tqchfdmy	50	28	et	et	NNP
cord-002791-tqchfdmy	50	29	al	al	NNP
cord-002791-tqchfdmy	50	30	.	.	.
cord-002791-tqchfdmy	50	31	and	and	CC
cord-002791-tqchfdmy	50	32	expression	expression	NN
cord-002791-tqchfdmy	50	33	of	of	IN
cord-002791-tqchfdmy	50	34	His	-PRON-	PRP$
cord-002791-tqchfdmy	50	35	-	-	HYPH
cord-002791-tqchfdmy	50	36	tagged	tag	VBN
cord-002791-tqchfdmy	50	37	MS2	MS2	NNP
cord-002791-tqchfdmy	50	38	PLP	PLP	NNP
cord-002791-tqchfdmy	50	39	in	in	IN
cord-002791-tqchfdmy	50	40	a	a	DT
cord-002791-tqchfdmy	50	41	one	one	CD
cord-002791-tqchfdmy	50	42	-	-	HYPH
cord-002791-tqchfdmy	50	43	plasmid	plasmid	NN
cord-002791-tqchfdmy	50	44	double	double	JJ
cord-002791-tqchfdmy	50	45	-	-	HYPH
cord-002791-tqchfdmy	50	46	expression	expression	NN
cord-002791-tqchfdmy	50	47	system	system	NN
cord-002791-tqchfdmy	50	48	does	do	VBZ
cord-002791-tqchfdmy	50	49	not	not	RB
cord-002791-tqchfdmy	50	50	result	result	VB
cord-002791-tqchfdmy	50	51	in	in	IN
cord-002791-tqchfdmy	50	52	the	the	DT
cord-002791-tqchfdmy	50	53	production	production	NN
cord-002791-tqchfdmy	50	54	of	of	IN
cord-002791-tqchfdmy	50	55	any	any	DT
cord-002791-tqchfdmy	50	56	His	His	NNP
cord-002791-tqchfdmy	50	57	-	-	HYPH
cord-002791-tqchfdmy	50	58	tagged	tag	VBN
cord-002791-tqchfdmy	50	59	MS2	MS2	NNP
cord-002791-tqchfdmy	50	60	PLP	PLP	NNP
cord-002791-tqchfdmy	50	61	13	13	CD
cord-002791-tqchfdmy	50	62	.	.	.
cord-002791-tqchfdmy	51	1	It	-PRON-	PRP
cord-002791-tqchfdmy	51	2	was	be	VBD
cord-002791-tqchfdmy	51	3	subsequently	subsequently	RB
cord-002791-tqchfdmy	51	4	discovered	discover	VBN
cord-002791-tqchfdmy	51	5	that	that	IN
cord-002791-tqchfdmy	51	6	another	another	DT
cord-002791-tqchfdmy	51	7	group	group	NN
cord-002791-tqchfdmy	51	8	also	also	RB
cord-002791-tqchfdmy	51	9	encountered	encounter	VBD
cord-002791-tqchfdmy	51	10	this	this	DT
cord-002791-tqchfdmy	51	11	problem	problem	NN
cord-002791-tqchfdmy	51	12	and	and	CC
cord-002791-tqchfdmy	51	13	failed	fail	VBD
cord-002791-tqchfdmy	51	14	in	in	IN
cord-002791-tqchfdmy	51	15	the	the	DT
cord-002791-tqchfdmy	51	16	production	production	NN
cord-002791-tqchfdmy	51	17	of	of	IN
cord-002791-tqchfdmy	51	18	His	-PRON-	PRP$
cord-002791-tqchfdmy	51	19	-	-	HYPH
cord-002791-tqchfdmy	51	20	tagged	tag	VBN
cord-002791-tqchfdmy	51	21	MS2	MS2	NNP
cord-002791-tqchfdmy	51	22	PLP	PLP	NNP
cord-002791-tqchfdmy	51	23	(	(	-LRB-
cord-002791-tqchfdmy	51	24	Harder	Harder	NNP
cord-002791-tqchfdmy	51	25	,	,	,
cord-002791-tqchfdmy	51	26	Friedrich	Friedrich	NNP
cord-002791-tqchfdmy	51	27	Loeffler	Loeffler	NNP
cord-002791-tqchfdmy	51	28	Institute	Institute	NNP
cord-002791-tqchfdmy	51	29	,	,	,
cord-002791-tqchfdmy	51	30	Germany	Germany	NNP
cord-002791-tqchfdmy	51	31	,	,	,
cord-002791-tqchfdmy	51	32	personal	personal	JJ
cord-002791-tqchfdmy	51	33	communication	communication	NN
cord-002791-tqchfdmy	51	34	,	,	,
cord-002791-tqchfdmy	51	35	March	March	NNP
cord-002791-tqchfdmy	51	36	2017	2017	CD
cord-002791-tqchfdmy	51	37	)	)	-RRB-
cord-002791-tqchfdmy	51	38	.	.	.
cord-002791-tqchfdmy	52	1	Ponchon	Ponchon	NNP
cord-002791-tqchfdmy	52	2	et	et	NNP
cord-002791-tqchfdmy	52	3	al	al	NNP
cord-002791-tqchfdmy	52	4	.	.	.
cord-002791-tqchfdmy	53	1	also	also	RB
cord-002791-tqchfdmy	53	2	adopted	adopt	VBD
cord-002791-tqchfdmy	53	3	the	the	DT
cord-002791-tqchfdmy	53	4	previously	previously	RB
cord-002791-tqchfdmy	53	5	reported	report	VBN
cord-002791-tqchfdmy	53	6	strategy	strategy	NN
cord-002791-tqchfdmy	53	7	of	of	IN
cord-002791-tqchfdmy	53	8	Cheng	Cheng	NNP
cord-002791-tqchfdmy	53	9	et	et	NNP
cord-002791-tqchfdmy	53	10	al	al	NNP
cord-002791-tqchfdmy	53	11	.	.	.
cord-002791-tqchfdmy	53	12	and	and	CC
cord-002791-tqchfdmy	53	13	tried	try	VBD
cord-002791-tqchfdmy	53	14	to	to	TO
cord-002791-tqchfdmy	53	15	produce	produce	VB
cord-002791-tqchfdmy	53	16	His	-PRON-	PRP$
cord-002791-tqchfdmy	53	17	-	-	HYPH
cord-002791-tqchfdmy	53	18	tagged	tag	VBN
cord-002791-tqchfdmy	53	19	MS2	MS2	NNP
cord-002791-tqchfdmy	53	20	PLP	PLP	NNP
cord-002791-tqchfdmy	53	21	15	15	CD
cord-002791-tqchfdmy	53	22	,	,	,
cord-002791-tqchfdmy	53	23	16	16	CD
cord-002791-tqchfdmy	53	24	.	.	.
cord-002791-tqchfdmy	54	1	However	however	RB
cord-002791-tqchfdmy	54	2	,	,	,
cord-002791-tqchfdmy	54	3	Ponchon	Ponchon	NNP
cord-002791-tqchfdmy	54	4	et	et	NNP
cord-002791-tqchfdmy	54	5	al	al	NNP
cord-002791-tqchfdmy	54	6	.	.	NNP
cord-002791-tqchfdmy	54	7	used	use	VBD
cord-002791-tqchfdmy	54	8	a	a	DT
cord-002791-tqchfdmy	54	9	so	so	RB
cord-002791-tqchfdmy	54	10	-	-	HYPH
cord-002791-tqchfdmy	54	11	called	call	VBN
cord-002791-tqchfdmy	54	12	two	two	CD
cord-002791-tqchfdmy	54	13	-	-	HYPH
cord-002791-tqchfdmy	54	14	plasmid	plasmid	NN
cord-002791-tqchfdmy	54	15	system	system	NN
cord-002791-tqchfdmy	54	16	where	where	WRB
cord-002791-tqchfdmy	54	17	the	the	DT
cord-002791-tqchfdmy	54	18	first	first	JJ
cord-002791-tqchfdmy	54	19	plasmid	plasmid	NN
cord-002791-tqchfdmy	54	20	carried	carry	VBD
cord-002791-tqchfdmy	54	21	the	the	DT
cord-002791-tqchfdmy	54	22	MS2	MS2	NNP
cord-002791-tqchfdmy	54	23	coat	coat	NN
cord-002791-tqchfdmy	54	24	protein	protein	NN
cord-002791-tqchfdmy	54	25	sequence	sequence	NN
cord-002791-tqchfdmy	54	26	under	under	IN
cord-002791-tqchfdmy	54	27	control	control	NN
cord-002791-tqchfdmy	54	28	of	of	IN
cord-002791-tqchfdmy	54	29	an	an	DT
cord-002791-tqchfdmy	54	30	inducible	inducible	JJ
cord-002791-tqchfdmy	54	31	tac	tac	NNP
cord-002791-tqchfdmy	54	32	promoter	promoter	NN
cord-002791-tqchfdmy	54	33	and	and	CC
cord-002791-tqchfdmy	54	34	the	the	DT
cord-002791-tqchfdmy	54	35	second	second	JJ
cord-002791-tqchfdmy	54	36	plasmid	plasmid	NN
cord-002791-tqchfdmy	54	37	carried	carry	VBD
cord-002791-tqchfdmy	54	38	the	the	DT
cord-002791-tqchfdmy	54	39	sequence	sequence	NN
cord-002791-tqchfdmy	54	40	for	for	IN
cord-002791-tqchfdmy	54	41	packaging	packaging	NN
cord-002791-tqchfdmy	54	42	under	under	IN
cord-002791-tqchfdmy	54	43	the	the	DT
cord-002791-tqchfdmy	54	44	control	control	NN
cord-002791-tqchfdmy	54	45	of	of	IN
cord-002791-tqchfdmy	54	46	a	a	DT
cord-002791-tqchfdmy	54	47	constitutive	constitutive	JJ
cord-002791-tqchfdmy	54	48	lpp	lpp	NN
cord-002791-tqchfdmy	54	49	promoter	promoter	NN
cord-002791-tqchfdmy	54	50	10	10	CD
cord-002791-tqchfdmy	54	51	,	,	,
cord-002791-tqchfdmy	54	52	16	16	CD
cord-002791-tqchfdmy	54	53	.	.	.
cord-002791-tqchfdmy	55	1	This	this	DT
cord-002791-tqchfdmy	55	2	strategy	strategy	NN
cord-002791-tqchfdmy	55	3	allows	allow	VBZ
cord-002791-tqchfdmy	55	4	successful	successful	JJ
cord-002791-tqchfdmy	55	5	co	co	JJ
cord-002791-tqchfdmy	55	6	-	-	NN
cord-002791-tqchfdmy	55	7	expression	expression	NN
cord-002791-tqchfdmy	55	8	of	of	IN
cord-002791-tqchfdmy	55	9	both	both	CC
cord-002791-tqchfdmy	55	10	the	the	DT
cord-002791-tqchfdmy	55	11	protein	protein	NN
cord-002791-tqchfdmy	55	12	and	and	CC
cord-002791-tqchfdmy	55	13	ssRNA	ssrna	NN
cord-002791-tqchfdmy	55	14	in	in	IN
cord-002791-tqchfdmy	55	15	an	an	DT
cord-002791-tqchfdmy	55	16	appropriate	appropriate	JJ
cord-002791-tqchfdmy	55	17	ratio	ratio	NN
cord-002791-tqchfdmy	55	18	.	.	.
cord-002791-tqchfdmy	56	1	But	but	CC
cord-002791-tqchfdmy	56	2	as	as	IN
cord-002791-tqchfdmy	56	3	a	a	DT
cord-002791-tqchfdmy	56	4	result	result	NN
cord-002791-tqchfdmy	56	5	,	,	,
cord-002791-tqchfdmy	56	6	in	in	IN
cord-002791-tqchfdmy	56	7	all	all	DT
cord-002791-tqchfdmy	56	8	cases	case	NNS
cord-002791-tqchfdmy	56	9	,	,	,
cord-002791-tqchfdmy	56	10	it	-PRON-	PRP
cord-002791-tqchfdmy	56	11	was	be	VBD
cord-002791-tqchfdmy	56	12	observed	observe	VBN
cord-002791-tqchfdmy	56	13	that	that	IN
cord-002791-tqchfdmy	56	14	the	the	DT
cord-002791-tqchfdmy	56	15	MS2	MS2	NNP
cord-002791-tqchfdmy	56	16	coat	coat	NN
cord-002791-tqchfdmy	56	17	protein	protein	NN
cord-002791-tqchfdmy	56	18	with	with	IN
cord-002791-tqchfdmy	56	19	the	the	DT
cord-002791-tqchfdmy	56	20	His	His	NNP
cord-002791-tqchfdmy	56	21	-	-	HYPH
cord-002791-tqchfdmy	56	22	tag	tag	NN
cord-002791-tqchfdmy	56	23	only	only	RB
cord-002791-tqchfdmy	56	24	formed	form	VBD
cord-002791-tqchfdmy	56	25	a	a	DT
cord-002791-tqchfdmy	56	26	dimer	dimer	NN
cord-002791-tqchfdmy	56	27	rather	rather	RB
cord-002791-tqchfdmy	56	28	than	than	IN
cord-002791-tqchfdmy	56	29	intact	intact	JJ
cord-002791-tqchfdmy	57	1	His	-PRON-	PRP$
cord-002791-tqchfdmy	57	2	-	-	HYPH
cord-002791-tqchfdmy	57	3	tagged	tag	VBN
cord-002791-tqchfdmy	57	4	MS2	MS2	NNP
cord-002791-tqchfdmy	57	5	PLP	PLP	NNP
cord-002791-tqchfdmy	57	6	16	16	CD
cord-002791-tqchfdmy	57	7	.	.	.
cord-002791-tqchfdmy	58	1	It	-PRON-	PRP
cord-002791-tqchfdmy	58	2	is	be	VBZ
cord-002791-tqchfdmy	58	3	known	know	VBN
cord-002791-tqchfdmy	58	4	that	that	IN
cord-002791-tqchfdmy	58	5	peptide	peptide	NN
cord-002791-tqchfdmy	58	6	insertions	insertion	NNS
cord-002791-tqchfdmy	58	7	into	into	IN
cord-002791-tqchfdmy	58	8	viral	viral	JJ
cord-002791-tqchfdmy	58	9	capsid	capsid	NN
cord-002791-tqchfdmy	58	10	proteins	protein	NNS
cord-002791-tqchfdmy	58	11	often	often	RB
cord-002791-tqchfdmy	58	12	result	result	VBP
cord-002791-tqchfdmy	58	13	in	in	IN
cord-002791-tqchfdmy	58	14	protein	protein	NN
cord-002791-tqchfdmy	58	15	misfolding	misfolding	NN
cord-002791-tqchfdmy	58	16	that	that	WDT
cord-002791-tqchfdmy	58	17	interferes	interfere	VBZ
cord-002791-tqchfdmy	58	18	with	with	IN
cord-002791-tqchfdmy	58	19	MS2	MS2	NNP
cord-002791-tqchfdmy	58	20	PLP	PLP	NNP
cord-002791-tqchfdmy	58	21	assembly	assembly	NN
cord-002791-tqchfdmy	58	22	17	17	CD
cord-002791-tqchfdmy	58	23	.	.	.
cord-002791-tqchfdmy	59	1	On	on	IN
cord-002791-tqchfdmy	59	2	the	the	DT
cord-002791-tqchfdmy	59	3	other	other	JJ
cord-002791-tqchfdmy	59	4	hand	hand	NN
cord-002791-tqchfdmy	59	5	,	,	,
cord-002791-tqchfdmy	59	6	it	-PRON-	PRP
cord-002791-tqchfdmy	59	7	is	be	VBZ
cord-002791-tqchfdmy	59	8	also	also	RB
cord-002791-tqchfdmy	59	9	known	know	VBN
cord-002791-tqchfdmy	59	10	that	that	DT
cord-002791-tqchfdmy	59	11	fusion	fusion	NN
cord-002791-tqchfdmy	59	12	of	of	IN
cord-002791-tqchfdmy	59	13	two	two	CD
cord-002791-tqchfdmy	59	14	coat	coat	NN
cord-002791-tqchfdmy	59	15	protein	protein	NN
cord-002791-tqchfdmy	59	16	monomers	monomer	NNS
cord-002791-tqchfdmy	59	17	(	(	-LRB-
cord-002791-tqchfdmy	59	18	single	single	JJ
cord-002791-tqchfdmy	59	19	-	-	HYPH
cord-002791-tqchfdmy	59	20	chain	chain	NN
cord-002791-tqchfdmy	59	21	dimer	dimer	NN
cord-002791-tqchfdmy	59	22	)	)	-RRB-
cord-002791-tqchfdmy	59	23	confers	confer	VBZ
cord-002791-tqchfdmy	59	24	tolerance	tolerance	NN
cord-002791-tqchfdmy	59	25	to	to	IN
cord-002791-tqchfdmy	59	26	peptide	peptide	JJ
cord-002791-tqchfdmy	59	27	insertions	insertion	NNS
cord-002791-tqchfdmy	59	28	in	in	IN
cord-002791-tqchfdmy	59	29	an	an	DT
cord-002791-tqchfdmy	59	30	MS2	MS2	NNP
cord-002791-tqchfdmy	59	31	coat	coat	NN
cord-002791-tqchfdmy	59	32	protein	protein	NN
cord-002791-tqchfdmy	59	33	dimer	dimer	NNP
cord-002791-tqchfdmy	59	34	18	18	CD
cord-002791-tqchfdmy	59	35	,	,	,
cord-002791-tqchfdmy	59	36	19	19	CD
cord-002791-tqchfdmy	59	37	.	.	.
cord-002791-tqchfdmy	60	1	The	the	DT
cord-002791-tqchfdmy	60	2	basic	basic	JJ
cord-002791-tqchfdmy	60	3	idea	idea	NN
cord-002791-tqchfdmy	60	4	of	of	IN
cord-002791-tqchfdmy	60	5	a	a	DT
cord-002791-tqchfdmy	60	6	using	use	VBG
cord-002791-tqchfdmy	60	7	a	a	DT
cord-002791-tqchfdmy	60	8	single	single	JJ
cord-002791-tqchfdmy	60	9	-	-	HYPH
cord-002791-tqchfdmy	60	10	chain	chain	NN
cord-002791-tqchfdmy	60	11	version	version	NN
cord-002791-tqchfdmy	60	12	of	of	IN
cord-002791-tqchfdmy	60	13	the	the	DT
cord-002791-tqchfdmy	60	14	MS2	MS2	NNP
cord-002791-tqchfdmy	60	15	coat	coat	NN
cord-002791-tqchfdmy	60	16	protein	protein	NN
cord-002791-tqchfdmy	60	17	dimer	dimer	NNP
cord-002791-tqchfdmy	60	18	with	with	IN
cord-002791-tqchfdmy	60	19	a	a	DT
cord-002791-tqchfdmy	60	20	His	-PRON-	PRP$
cord-002791-tqchfdmy	60	21	-	-	HYPH
cord-002791-tqchfdmy	60	22	tag	tag	NN
cord-002791-tqchfdmy	60	23	in	in	IN
cord-002791-tqchfdmy	60	24	this	this	DT
cord-002791-tqchfdmy	60	25	study	study	NN
cord-002791-tqchfdmy	60	26	was	be	VBD
cord-002791-tqchfdmy	60	27	inspired	inspire	VBN
cord-002791-tqchfdmy	60	28	by	by	IN
cord-002791-tqchfdmy	60	29	these	these	DT
cord-002791-tqchfdmy	60	30	previously	previously	RB
cord-002791-tqchfdmy	60	31	published	publish	VBN
cord-002791-tqchfdmy	60	32	results	result	NNS
cord-002791-tqchfdmy	60	33	.	.	.
cord-002791-tqchfdmy	61	1	The	the	DT
cord-002791-tqchfdmy	61	2	described	describe	VBN
cord-002791-tqchfdmy	61	3	plasmid	plasmid	NN
cord-002791-tqchfdmy	61	4	-	-	HYPH
cord-002791-tqchfdmy	61	5	driven	drive	VBN
cord-002791-tqchfdmy	61	6	system	system	NN
cord-002791-tqchfdmy	61	7	is	be	VBZ
cord-002791-tqchfdmy	61	8	based	base	VBN
cord-002791-tqchfdmy	61	9	on	on	IN
cord-002791-tqchfdmy	61	10	plasmid	plasmid	NN
cord-002791-tqchfdmy	61	11	pACYCDuet-1	pACYCDuet-1	NNP
cord-002791-tqchfdmy	61	12	.	.	.
cord-002791-tqchfdmy	62	1	Plasmid	Plasmid	NNP
cord-002791-tqchfdmy	62	2	pACYCDuet-1	pACYCDuet-1	NNP
cord-002791-tqchfdmy	62	3	was	be	VBD
cord-002791-tqchfdmy	62	4	chosen	choose	VBN
cord-002791-tqchfdmy	62	5	as	as	IN
cord-002791-tqchfdmy	62	6	the	the	DT
cord-002791-tqchfdmy	62	7	basis	basis	NN
cord-002791-tqchfdmy	62	8	for	for	IN
cord-002791-tqchfdmy	62	9	the	the	DT
cord-002791-tqchfdmy	62	10	new	new	JJ
cord-002791-tqchfdmy	62	11	His	-PRON-	PRP$
cord-002791-tqchfdmy	62	12	-	-	HYPH
cord-002791-tqchfdmy	62	13	tagged	tag	VBN
cord-002791-tqchfdmy	62	14	MS2	MS2	NNP
cord-002791-tqchfdmy	62	15	PLP	PLP	NNP
cord-002791-tqchfdmy	62	16	production	production	NN
cord-002791-tqchfdmy	62	17	system	system	NN
cord-002791-tqchfdmy	62	18	because	because	IN
cord-002791-tqchfdmy	62	19	it	-PRON-	PRP
cord-002791-tqchfdmy	62	20	was	be	VBD
cord-002791-tqchfdmy	62	21	shown	show	VBN
cord-002791-tqchfdmy	62	22	to	to	TO
cord-002791-tqchfdmy	62	23	be	be	VB
cord-002791-tqchfdmy	62	24	an	an	DT
cord-002791-tqchfdmy	62	25	optimal	optimal	JJ
cord-002791-tqchfdmy	62	26	solution	solution	NN
cord-002791-tqchfdmy	62	27	in	in	IN
cord-002791-tqchfdmy	62	28	previous	previous	JJ
cord-002791-tqchfdmy	62	29	studies	study	NNS
cord-002791-tqchfdmy	62	30	that	that	WDT
cord-002791-tqchfdmy	62	31	dealt	deal	VBD
cord-002791-tqchfdmy	62	32	with	with	IN
cord-002791-tqchfdmy	62	33	the	the	DT
cord-002791-tqchfdmy	62	34	construction	construction	NN
cord-002791-tqchfdmy	62	35	of	of	IN
cord-002791-tqchfdmy	62	36	particles	particle	NNS
cord-002791-tqchfdmy	62	37	or	or	CC
cord-002791-tqchfdmy	62	38	production	production	NN
cord-002791-tqchfdmy	62	39	of	of	IN
cord-002791-tqchfdmy	62	40	MS2	MS2	NNP
cord-002791-tqchfdmy	62	41	coat	coat	NN
cord-002791-tqchfdmy	62	42	protein	protein	NNP
cord-002791-tqchfdmy	62	43	11	11	CD
cord-002791-tqchfdmy	62	44	,	,	,
cord-002791-tqchfdmy	62	45	[	[	-LRB-
cord-002791-tqchfdmy	62	46	20	20	CD
cord-002791-tqchfdmy	62	47	]	]	-RRB-
cord-002791-tqchfdmy	62	48	[	[	-LRB-
cord-002791-tqchfdmy	62	49	21	21	CD
cord-002791-tqchfdmy	62	50	]	]	-RRB-
cord-002791-tqchfdmy	62	51	[	[	-LRB-
cord-002791-tqchfdmy	62	52	22	22	CD
cord-002791-tqchfdmy	62	53	]	]	-RRB-
cord-002791-tqchfdmy	62	54	.	.	.
cord-002791-tqchfdmy	63	1	Zhan	Zhan	NNP
cord-002791-tqchfdmy	63	2	et	et	NNP
cord-002791-tqchfdmy	63	3	al	al	NNP
cord-002791-tqchfdmy	63	4	.	.	NNP
cord-002791-tqchfdmy	63	5	used	use	VBD
cord-002791-tqchfdmy	63	6	a	a	DT
cord-002791-tqchfdmy	63	7	one	one	CD
cord-002791-tqchfdmy	63	8	-	-	HYPH
cord-002791-tqchfdmy	63	9	plasmid	plasmid	NN
cord-002791-tqchfdmy	63	10	double	double	JJ
cord-002791-tqchfdmy	63	11	-	-	HYPH
cord-002791-tqchfdmy	63	12	expression	expression	NN
cord-002791-tqchfdmy	63	13	system	system	NN
cord-002791-tqchfdmy	63	14	based	base	VBN
cord-002791-tqchfdmy	63	15	on	on	IN
cord-002791-tqchfdmy	63	16	the	the	DT
cord-002791-tqchfdmy	63	17	pACYCDuet-1	pacycduet-1	CD
cord-002791-tqchfdmy	63	18	plasmid	plasmid	NN
cord-002791-tqchfdmy	63	19	for	for	IN
cord-002791-tqchfdmy	63	20	production	production	NN
cord-002791-tqchfdmy	63	21	of	of	IN
cord-002791-tqchfdmy	63	22	MS2	MS2	NNP
cord-002791-tqchfdmy	63	23	PLP	PLP	NNP
cord-002791-tqchfdmy	63	24	carrying	carry	VBG
cord-002791-tqchfdmy	63	25	a	a	DT
cord-002791-tqchfdmy	63	26	3,034-nt	3,034-nt	CD
cord-002791-tqchfdmy	63	27	-	-	HYPH
cord-002791-tqchfdmy	63	28	long	long	JJ
cord-002791-tqchfdmy	63	29	control	control	NN
cord-002791-tqchfdmy	63	30	sequence	sequence	NN
cord-002791-tqchfdmy	63	31	11	11	CD
cord-002791-tqchfdmy	63	32	.	.	.
cord-002791-tqchfdmy	64	1	To	to	TO
cord-002791-tqchfdmy	64	2	test	test	VB
cord-002791-tqchfdmy	64	3	the	the	DT
cord-002791-tqchfdmy	64	4	ability	ability	NN
cord-002791-tqchfdmy	64	5	of	of	IN
cord-002791-tqchfdmy	64	6	such	such	PDT
cord-002791-tqchfdmy	64	7	a	a	DT
cord-002791-tqchfdmy	64	8	system	system	NN
cord-002791-tqchfdmy	64	9	to	to	TO
cord-002791-tqchfdmy	64	10	also	also	RB
cord-002791-tqchfdmy	64	11	pack	pack	VB
cord-002791-tqchfdmy	64	12	very	very	RB
cord-002791-tqchfdmy	64	13	short	short	JJ
cord-002791-tqchfdmy	64	14	control	control	NN
cord-002791-tqchfdmy	64	15	sequences	sequence	NNS
cord-002791-tqchfdmy	64	16	,	,	,
cord-002791-tqchfdmy	64	17	our	-PRON-	PRP$
cord-002791-tqchfdmy	64	18	previously	previously	RB
cord-002791-tqchfdmy	64	19	reported	report	VBN
cord-002791-tqchfdmy	64	20	specific	specific	JJ
cord-002791-tqchfdmy	64	21	control	control	NN
cord-002791-tqchfdmy	64	22	sequence	sequence	NN
cord-002791-tqchfdmy	64	23	with	with	IN
cord-002791-tqchfdmy	64	24	a	a	DT
cord-002791-tqchfdmy	64	25	total	total	JJ
cord-002791-tqchfdmy	64	26	length	length	NN
cord-002791-tqchfdmy	64	27	of	of	IN
cord-002791-tqchfdmy	64	28	150	150	CD
cord-002791-tqchfdmy	64	29	nt	nt	RB
cord-002791-tqchfdmy	64	30	was	be	VBD
cord-002791-tqchfdmy	64	31	chosen	choose	VBN
cord-002791-tqchfdmy	64	32	for	for	IN
cord-002791-tqchfdmy	64	33	packaging	packaging	NN
cord-002791-tqchfdmy	64	34	8	8	CD
cord-002791-tqchfdmy	64	35	.	.	.
cord-002791-tqchfdmy	65	1	The	the	DT
cord-002791-tqchfdmy	65	2	final	final	JJ
cord-002791-tqchfdmy	65	3	pACYCDuet-1-TM	pacycduet-1-tm	CD
cord-002791-tqchfdmy	65	4	-	-	HYPH
cord-002791-tqchfdmy	65	5	CoatDimer	CoatDimer	NNP
cord-002791-tqchfdmy	65	6	-	-	:
cord-002791-tqchfdmy	65	7	His	-PRON-	PRP$
cord-002791-tqchfdmy	65	8	expression	expression	NN
cord-002791-tqchfdmy	65	9	vector	vector	NN
cord-002791-tqchfdmy	65	10	was	be	VBD
cord-002791-tqchfdmy	65	11	constructed	construct	VBN
cord-002791-tqchfdmy	65	12	with	with	IN
cord-002791-tqchfdmy	65	13	a	a	DT
cord-002791-tqchfdmy	65	14	view	view	NN
cord-002791-tqchfdmy	65	15	of	of	IN
cord-002791-tqchfdmy	65	16	easily	easily	RB
cord-002791-tqchfdmy	65	17	allowing	allow	VBG
cord-002791-tqchfdmy	65	18	subsequent	subsequent	JJ
cord-002791-tqchfdmy	65	19	modifications	modification	NNS
cord-002791-tqchfdmy	65	20	.	.	.
cord-002791-tqchfdmy	66	1	MCS1	MCS1	NNP
cord-002791-tqchfdmy	66	2	of	of	IN
cord-002791-tqchfdmy	66	3	the	the	DT
cord-002791-tqchfdmy	66	4	vector	vector	NN
cord-002791-tqchfdmy	66	5	containing	contain	VBG
cord-002791-tqchfdmy	66	6	the	the	DT
cord-002791-tqchfdmy	66	7	sequences	sequence	NNS
cord-002791-tqchfdmy	66	8	of	of	IN
cord-002791-tqchfdmy	66	9	maturase	maturase	NN
cord-002791-tqchfdmy	66	10	and	and	CC
cord-002791-tqchfdmy	66	11	single	single	JJ
cord-002791-tqchfdmy	66	12	-	-	HYPH
cord-002791-tqchfdmy	66	13	chain	chain	NN
cord-002791-tqchfdmy	66	14	version	version	NN
cord-002791-tqchfdmy	66	15	of	of	IN
cord-002791-tqchfdmy	66	16	the	the	DT
cord-002791-tqchfdmy	66	17	coat	coat	NN
cord-002791-tqchfdmy	66	18	protein	protein	NN
cord-002791-tqchfdmy	66	19	dimer	dimer	NN
cord-002791-tqchfdmy	66	20	containing	contain	VBG
cord-002791-tqchfdmy	66	21	the	the	DT
cord-002791-tqchfdmy	66	22	His	-PRON-	PRP$
cord-002791-tqchfdmy	66	23	-	-	HYPH
cord-002791-tqchfdmy	66	24	tag	tag	NN
cord-002791-tqchfdmy	66	25	was	be	VBD
cord-002791-tqchfdmy	66	26	modified	modify	VBN
cord-002791-tqchfdmy	66	27	,	,	,
cord-002791-tqchfdmy	66	28	so	so	IN
cord-002791-tqchfdmy	66	29	that	that	IN
cord-002791-tqchfdmy	66	30	the	the	DT
cord-002791-tqchfdmy	66	31	maturase	maturase	NN
cord-002791-tqchfdmy	66	32	sequence	sequence	NN
cord-002791-tqchfdmy	66	33	no	no	RB
cord-002791-tqchfdmy	66	34	longer	long	RBR
cord-002791-tqchfdmy	66	35	contains	contain	VBZ
cord-002791-tqchfdmy	66	36	the	the	DT
cord-002791-tqchfdmy	66	37	NdeI	NdeI	NNP
cord-002791-tqchfdmy	66	38	restriction	restriction	NN
cord-002791-tqchfdmy	66	39	site	site	NN
cord-002791-tqchfdmy	66	40	.	.	.
cord-002791-tqchfdmy	67	1	This	this	DT
cord-002791-tqchfdmy	67	2	modification	modification	NN
cord-002791-tqchfdmy	67	3	opens	open	VBZ
cord-002791-tqchfdmy	67	4	the	the	DT
cord-002791-tqchfdmy	67	5	possibility	possibility	NN
cord-002791-tqchfdmy	67	6	for	for	IN
cord-002791-tqchfdmy	67	7	using	use	VBG
cord-002791-tqchfdmy	67	8	the	the	DT
cord-002791-tqchfdmy	67	9	NdeI	NdeI	NNP
cord-002791-tqchfdmy	67	10	restriction	restriction	NN
cord-002791-tqchfdmy	67	11	site	site	NN
cord-002791-tqchfdmy	67	12	for	for	IN
cord-002791-tqchfdmy	67	13	cloning	clone	VBG
cord-002791-tqchfdmy	67	14	into	into	IN
cord-002791-tqchfdmy	67	15	MCS2	MCS2	NNP
cord-002791-tqchfdmy	67	16	,	,	,
cord-002791-tqchfdmy	67	17	which	which	WDT
cord-002791-tqchfdmy	67	18	increases	increase	VBZ
cord-002791-tqchfdmy	67	19	the	the	DT
cord-002791-tqchfdmy	67	20	maximum	maximum	JJ
cord-002791-tqchfdmy	67	21	length	length	NN
cord-002791-tqchfdmy	67	22	of	of	IN
cord-002791-tqchfdmy	67	23	packed	packed	JJ
cord-002791-tqchfdmy	67	24	ssRNA	ssrna	NN
cord-002791-tqchfdmy	67	25	control	control	NN
cord-002791-tqchfdmy	67	26	sequences	sequence	NNS
cord-002791-tqchfdmy	67	27	,	,	,
cord-002791-tqchfdmy	67	28	because	because	IN
cord-002791-tqchfdmy	67	29	NdeI	NdeI	NNP
cord-002791-tqchfdmy	67	30	is	be	VBZ
cord-002791-tqchfdmy	67	31	the	the	DT
cord-002791-tqchfdmy	67	32	first	first	JJ
cord-002791-tqchfdmy	67	33	restriction	restriction	NN
cord-002791-tqchfdmy	67	34	site	site	NN
cord-002791-tqchfdmy	67	35	in	in	IN
cord-002791-tqchfdmy	67	36	the	the	DT
cord-002791-tqchfdmy	67	37	MCS2	MCS2	NNP
cord-002791-tqchfdmy	67	38	of	of	IN
cord-002791-tqchfdmy	67	39	the	the	DT
cord-002791-tqchfdmy	67	40	vector	vector	NN
cord-002791-tqchfdmy	67	41	.	.	.
cord-002791-tqchfdmy	68	1	Furthermore	furthermore	RB
cord-002791-tqchfdmy	68	2	,	,	,
cord-002791-tqchfdmy	68	3	in	in	IN
cord-002791-tqchfdmy	68	4	the	the	DT
cord-002791-tqchfdmy	68	5	case	case	NN
cord-002791-tqchfdmy	68	6	that	that	IN
cord-002791-tqchfdmy	68	7	the	the	DT
cord-002791-tqchfdmy	68	8	NdeI	NdeI	NNP
cord-002791-tqchfdmy	68	9	sequence	sequence	NN
cord-002791-tqchfdmy	68	10	will	will	MD
cord-002791-tqchfdmy	68	11	be	be	VB
cord-002791-tqchfdmy	68	12	present	present	JJ
cord-002791-tqchfdmy	68	13	in	in	IN
cord-002791-tqchfdmy	68	14	the	the	DT
cord-002791-tqchfdmy	68	15	sequence	sequence	NN
cord-002791-tqchfdmy	68	16	of	of	IN
cord-002791-tqchfdmy	68	17	maturase	maturase	NN
cord-002791-tqchfdmy	68	18	,	,	,
cord-002791-tqchfdmy	68	19	direct	direct	JJ
cord-002791-tqchfdmy	68	20	cloning	cloning	NN
cord-002791-tqchfdmy	68	21	of	of	IN
cord-002791-tqchfdmy	68	22	sequences	sequence	NNS
cord-002791-tqchfdmy	68	23	into	into	IN
cord-002791-tqchfdmy	68	24	MCS2	MCS2	NNP
cord-002791-tqchfdmy	68	25	of	of	IN
cord-002791-tqchfdmy	68	26	the	the	DT
cord-002791-tqchfdmy	68	27	vector	vector	NN
cord-002791-tqchfdmy	68	28	using	use	VBG
cord-002791-tqchfdmy	68	29	the	the	DT
cord-002791-tqchfdmy	68	30	NdeI	NdeI	NNP
cord-002791-tqchfdmy	68	31	restriction	restriction	NN
cord-002791-tqchfdmy	68	32	site	site	NN
cord-002791-tqchfdmy	68	33	will	will	MD
cord-002791-tqchfdmy	68	34	not	not	RB
cord-002791-tqchfdmy	68	35	be	be	VB
cord-002791-tqchfdmy	68	36	possible	possible	JJ
cord-002791-tqchfdmy	68	37	.	.	.
cord-002791-tqchfdmy	69	1	The	the	DT
cord-002791-tqchfdmy	69	2	inserted	insert	VBN
cord-002791-tqchfdmy	69	3	sequence	sequence	NN
cord-002791-tqchfdmy	69	4	for	for	IN
cord-002791-tqchfdmy	69	5	the	the	DT
cord-002791-tqchfdmy	69	6	His	His	NNP
cord-002791-tqchfdmy	69	7	-	-	HYPH
cord-002791-tqchfdmy	69	8	tag	tag	NN
cord-002791-tqchfdmy	69	9	is	be	VBZ
cord-002791-tqchfdmy	69	10	flanked	flank	VBN
cord-002791-tqchfdmy	69	11	by	by	IN
cord-002791-tqchfdmy	69	12	two	two	CD
cord-002791-tqchfdmy	69	13	KpnI	KpnI	NNP
cord-002791-tqchfdmy	69	14	restriction	restriction	NN
cord-002791-tqchfdmy	69	15	sites	site	NNS
cord-002791-tqchfdmy	69	16	.	.	.
cord-002791-tqchfdmy	70	1	This	this	DT
cord-002791-tqchfdmy	70	2	flanking	flanking	NN
cord-002791-tqchfdmy	70	3	is	be	VBZ
cord-002791-tqchfdmy	70	4	a	a	DT
cord-002791-tqchfdmy	70	5	consequence	consequence	NN
cord-002791-tqchfdmy	70	6	of	of	IN
cord-002791-tqchfdmy	70	7	previously	previously	RB
cord-002791-tqchfdmy	70	8	used	use	VBN
cord-002791-tqchfdmy	70	9	methods	method	NNS
cord-002791-tqchfdmy	70	10	of	of	IN
cord-002791-tqchfdmy	70	11	oligonucleotide	oligonucleotide	RB
cord-002791-tqchfdmy	70	12	-	-	HYPH
cord-002791-tqchfdmy	70	13	directed	direct	VBN
cord-002791-tqchfdmy	70	14	mutagenesis	mutagenesis	NN
cord-002791-tqchfdmy	70	15	14	14	CD
cord-002791-tqchfdmy	70	16	,	,	,
cord-002791-tqchfdmy	70	17	23	23	CD
cord-002791-tqchfdmy	70	18	.	.	.
cord-002791-tqchfdmy	71	1	Although	although	IN
cord-002791-tqchfdmy	71	2	the	the	DT
cord-002791-tqchfdmy	71	3	sequence	sequence	NN
cord-002791-tqchfdmy	71	4	of	of	IN
cord-002791-tqchfdmy	71	5	maturase	maturase	NN
cord-002791-tqchfdmy	71	6	and	and	CC
cord-002791-tqchfdmy	71	7	the	the	DT
cord-002791-tqchfdmy	71	8	single	single	JJ
cord-002791-tqchfdmy	71	9	-	-	HYPH
cord-002791-tqchfdmy	71	10	chain	chain	NN
cord-002791-tqchfdmy	71	11	version	version	NN
cord-002791-tqchfdmy	71	12	of	of	IN
cord-002791-tqchfdmy	71	13	coat	coat	NNP
cord-002791-tqchfdmy	71	14	protein	protein	NN
cord-002791-tqchfdmy	71	15	dimer	dimer	NN
cord-002791-tqchfdmy	71	16	containing	contain	VBG
cord-002791-tqchfdmy	71	17	the	the	DT
cord-002791-tqchfdmy	71	18	His	-PRON-	PRP$
cord-002791-tqchfdmy	71	19	-	-	HYPH
cord-002791-tqchfdmy	71	20	tag	tag	NN
cord-002791-tqchfdmy	71	21	was	be	VBD
cord-002791-tqchfdmy	71	22	synthesized	synthesize	VBN
cord-002791-tqchfdmy	71	23	de	de	IN
cord-002791-tqchfdmy	71	24	novo	novo	NNP
cord-002791-tqchfdmy	71	25	,	,	,
cord-002791-tqchfdmy	71	26	it	-PRON-	PRP
cord-002791-tqchfdmy	71	27	was	be	VBD
cord-002791-tqchfdmy	71	28	decided	decide	VBN
cord-002791-tqchfdmy	71	29	to	to	TO
cord-002791-tqchfdmy	71	30	maintain	maintain	VB
cord-002791-tqchfdmy	71	31	the	the	DT
cord-002791-tqchfdmy	71	32	presence	presence	NN
cord-002791-tqchfdmy	71	33	of	of	IN
cord-002791-tqchfdmy	71	34	the	the	DT
cord-002791-tqchfdmy	71	35	KpnI	KpnI	NNP
cord-002791-tqchfdmy	71	36	restriction	restriction	NN
cord-002791-tqchfdmy	71	37	sites	site	NNS
cord-002791-tqchfdmy	71	38	,	,	,
cord-002791-tqchfdmy	71	39	mainly	mainly	RB
cord-002791-tqchfdmy	71	40	due	due	IN
cord-002791-tqchfdmy	71	41	to	to	IN
cord-002791-tqchfdmy	71	42	the	the	DT
cord-002791-tqchfdmy	71	43	possibility	possibility	NN
cord-002791-tqchfdmy	71	44	of	of	IN
cord-002791-tqchfdmy	71	45	easy	easy	JJ
cord-002791-tqchfdmy	71	46	replacement	replacement	NN
cord-002791-tqchfdmy	71	47	of	of	IN
cord-002791-tqchfdmy	71	48	the	the	DT
cord-002791-tqchfdmy	71	49	His	-PRON-	PRP$
cord-002791-tqchfdmy	71	50	-	-	HYPH
cord-002791-tqchfdmy	71	51	tag	tag	NN
cord-002791-tqchfdmy	71	52	sequence	sequence	NN
cord-002791-tqchfdmy	71	53	at	at	IN
cord-002791-tqchfdmy	71	54	this	this	DT
cord-002791-tqchfdmy	71	55	position	position	NN
cord-002791-tqchfdmy	71	56	.	.	.
cord-002791-tqchfdmy	72	1	The	the	DT
cord-002791-tqchfdmy	72	2	single	single	JJ
cord-002791-tqchfdmy	72	3	-	-	HYPH
cord-002791-tqchfdmy	72	4	chain	chain	NN
cord-002791-tqchfdmy	72	5	version	version	NN
cord-002791-tqchfdmy	72	6	of	of	IN
cord-002791-tqchfdmy	72	7	the	the	DT
cord-002791-tqchfdmy	72	8	coat	coat	NN
cord-002791-tqchfdmy	72	9	protein	protein	NN
cord-002791-tqchfdmy	72	10	dimer	dimer	NNP
cord-002791-tqchfdmy	72	11	is	be	VBZ
cord-002791-tqchfdmy	72	12	more	more	RBR
cord-002791-tqchfdmy	72	13	tolerant	tolerant	JJ
cord-002791-tqchfdmy	72	14	of	of	IN
cord-002791-tqchfdmy	72	15	foreign	foreign	JJ
cord-002791-tqchfdmy	72	16	peptide	peptide	NN
cord-002791-tqchfdmy	72	17	insertions	insertion	NNS
cord-002791-tqchfdmy	72	18	than	than	IN
cord-002791-tqchfdmy	72	19	the	the	DT
cord-002791-tqchfdmy	72	20	wild	wild	JJ
cord-002791-tqchfdmy	72	21	-	-	HYPH
cord-002791-tqchfdmy	72	22	type	type	NN
cord-002791-tqchfdmy	72	23	coat	coat	NN
cord-002791-tqchfdmy	72	24	protein	protein	NN
cord-002791-tqchfdmy	72	25	monomer	monomer	NN
cord-002791-tqchfdmy	72	26	18	18	CD
cord-002791-tqchfdmy	72	27	,	,	,
cord-002791-tqchfdmy	72	28	19	19	CD
cord-002791-tqchfdmy	72	29	.	.	.
cord-002791-tqchfdmy	73	1	Therefore	therefore	RB
cord-002791-tqchfdmy	73	2	,	,	,
cord-002791-tqchfdmy	73	3	utilization	utilization	NN
cord-002791-tqchfdmy	73	4	of	of	IN
cord-002791-tqchfdmy	73	5	the	the	DT
cord-002791-tqchfdmy	73	6	described	describe	VBN
cord-002791-tqchfdmy	73	7	system	system	NN
cord-002791-tqchfdmy	73	8	should	should	MD
cord-002791-tqchfdmy	73	9	allow	allow	VB
cord-002791-tqchfdmy	73	10	insertion	insertion	NN
cord-002791-tqchfdmy	73	11	of	of	IN
cord-002791-tqchfdmy	73	12	different	different	JJ
cord-002791-tqchfdmy	73	13	peptides	peptide	NNS
cord-002791-tqchfdmy	73	14	at	at	IN
cord-002791-tqchfdmy	73	15	this	this	DT
cord-002791-tqchfdmy	73	16	position	position	NN
cord-002791-tqchfdmy	73	17	without	without	IN
cord-002791-tqchfdmy	73	18	causing	cause	VBG
cord-002791-tqchfdmy	73	19	any	any	DT
cord-002791-tqchfdmy	73	20	problems	problem	NNS
cord-002791-tqchfdmy	73	21	for	for	IN
cord-002791-tqchfdmy	73	22	MS2	MS2	NNP
cord-002791-tqchfdmy	73	23	PLP	PLP	NNP
cord-002791-tqchfdmy	73	24	assembly	assembly	NN
cord-002791-tqchfdmy	73	25	.	.	.
cord-002791-tqchfdmy	74	1	The	the	DT
cord-002791-tqchfdmy	74	2	possibility	possibility	NN
cord-002791-tqchfdmy	74	3	of	of	IN
cord-002791-tqchfdmy	74	4	producing	produce	VBG
cord-002791-tqchfdmy	74	5	MS2	MS2	NNP
cord-002791-tqchfdmy	74	6	PLP	PLP	NNP
cord-002791-tqchfdmy	74	7	carrying	carry	VBG
cord-002791-tqchfdmy	74	8	ssRNAs	ssRNAs	NNP
cord-002791-tqchfdmy	74	9	of	of	IN
cord-002791-tqchfdmy	74	10	choice	choice	NN
cord-002791-tqchfdmy	74	11	and	and	CC
cord-002791-tqchfdmy	74	12	presenting	present	VBG
cord-002791-tqchfdmy	74	13	various	various	JJ
cord-002791-tqchfdmy	74	14	peptides	peptide	NNS
cord-002791-tqchfdmy	74	15	on	on	IN
cord-002791-tqchfdmy	74	16	the	the	DT
cord-002791-tqchfdmy	74	17	surface	surface	NN
cord-002791-tqchfdmy	74	18	of	of	IN
cord-002791-tqchfdmy	74	19	the	the	DT
cord-002791-tqchfdmy	74	20	capsid	capsid	NN
cord-002791-tqchfdmy	74	21	is	be	VBZ
cord-002791-tqchfdmy	74	22	currently	currently	RB
cord-002791-tqchfdmy	74	23	a	a	DT
cord-002791-tqchfdmy	74	24	very	very	RB
cord-002791-tqchfdmy	74	25	promising	promising	JJ
cord-002791-tqchfdmy	74	26	method	method	NN
cord-002791-tqchfdmy	74	27	for	for	IN
cord-002791-tqchfdmy	74	28	the	the	DT
cord-002791-tqchfdmy	74	29	production	production	NN
cord-002791-tqchfdmy	74	30	of	of	IN
cord-002791-tqchfdmy	74	31	RNA	RNA	NNP
cord-002791-tqchfdmy	74	32	vaccines	vaccine	NNS
cord-002791-tqchfdmy	74	33	based	base	VBN
cord-002791-tqchfdmy	74	34	on	on	IN
cord-002791-tqchfdmy	74	35	recombinant	recombinant	JJ
cord-002791-tqchfdmy	74	36	MS2	MS2	NNP
cord-002791-tqchfdmy	74	37	PLP	PLP	NNP
cord-002791-tqchfdmy	74	38	24	24	CD
cord-002791-tqchfdmy	74	39	-	-	SYM
cord-002791-tqchfdmy	74	40	28	28	CD
cord-002791-tqchfdmy	74	41	.	.	.
cord-002791-tqchfdmy	75	1	The	the	DT
cord-002791-tqchfdmy	75	2	single	single	JJ
cord-002791-tqchfdmy	75	3	-	-	HYPH
cord-002791-tqchfdmy	75	4	chain	chain	NN
cord-002791-tqchfdmy	75	5	version	version	NN
cord-002791-tqchfdmy	75	6	of	of	IN
cord-002791-tqchfdmy	75	7	the	the	DT
cord-002791-tqchfdmy	75	8	MS2	MS2	NNP
cord-002791-tqchfdmy	75	9	coat	coat	NN
cord-002791-tqchfdmy	75	10	protein	protein	NNP
cord-002791-tqchfdmy	75	11	dimer	dimer	NNP
cord-002791-tqchfdmy	75	12	contains	contain	VBZ
cord-002791-tqchfdmy	75	13	one	one	CD
cord-002791-tqchfdmy	75	14	wild	wild	JJ
cord-002791-tqchfdmy	75	15	-	-	HYPH
cord-002791-tqchfdmy	75	16	type	type	NN
cord-002791-tqchfdmy	75	17	coat	coat	NN
cord-002791-tqchfdmy	75	18	protein	protein	NN
cord-002791-tqchfdmy	75	19	fused	fuse	VBD
cord-002791-tqchfdmy	75	20	with	with	IN
cord-002791-tqchfdmy	75	21	one	one	CD
cord-002791-tqchfdmy	75	22	mutagenized	mutagenized	JJ
cord-002791-tqchfdmy	75	23	coat	coat	NN
cord-002791-tqchfdmy	75	24	protein	protein	NN
cord-002791-tqchfdmy	75	25	carrying	carry	VBG
cord-002791-tqchfdmy	75	26	the	the	DT
cord-002791-tqchfdmy	75	27	His	His	NNP
cord-002791-tqchfdmy	75	28	-	-	HYPH
cord-002791-tqchfdmy	75	29	tag	tag	NN
cord-002791-tqchfdmy	75	30	.	.	.
cord-002791-tqchfdmy	76	1	Therefore	therefore	RB
cord-002791-tqchfdmy	76	2	,	,	,
cord-002791-tqchfdmy	76	3	the	the	DT
cord-002791-tqchfdmy	76	4	presented	present	VBN
cord-002791-tqchfdmy	76	5	method	method	NN
cord-002791-tqchfdmy	76	6	results	result	NNS
cord-002791-tqchfdmy	76	7	in	in	IN
cord-002791-tqchfdmy	76	8	the	the	DT
cord-002791-tqchfdmy	76	9	production	production	NN
cord-002791-tqchfdmy	76	10	of	of	IN
cord-002791-tqchfdmy	76	11	His	-PRON-	PRP$
cord-002791-tqchfdmy	76	12	-	-	HYPH
cord-002791-tqchfdmy	76	13	tagged	tag	VBN
cord-002791-tqchfdmy	76	14	MS2	MS2	NNP
cord-002791-tqchfdmy	76	15	PLP	PLP	NNP
cord-002791-tqchfdmy	76	16	containing	contain	VBG
cord-002791-tqchfdmy	76	17	90	90	CD
cord-002791-tqchfdmy	76	18	His	-PRON-	PRP$
cord-002791-tqchfdmy	76	19	-	-	HYPH
cord-002791-tqchfdmy	76	20	tags	tag	NNS
cord-002791-tqchfdmy	76	21	in	in	IN
cord-002791-tqchfdmy	76	22	the	the	DT
cord-002791-tqchfdmy	76	23	capsid	capsid	JJ
cord-002791-tqchfdmy	76	24	structure	structure	NN
cord-002791-tqchfdmy	76	25	exposed	expose	VBN
cord-002791-tqchfdmy	76	26	outward	outward	RB
cord-002791-tqchfdmy	76	27	from	from	IN
cord-002791-tqchfdmy	76	28	the	the	DT
cord-002791-tqchfdmy	76	29	particle	particle	NN
cord-002791-tqchfdmy	76	30	and	and	CC
cord-002791-tqchfdmy	76	31	allowing	allow	VBG
cord-002791-tqchfdmy	76	32	chelated	chelate	VBN
cord-002791-tqchfdmy	76	33	Co	Co	NNP
cord-002791-tqchfdmy	76	34	2	2	CD
cord-002791-tqchfdmy	76	35	+	+	SYM
cord-002791-tqchfdmy	76	36	on	on	IN
cord-002791-tqchfdmy	76	37	the	the	DT
cord-002791-tqchfdmy	76	38	resin	resin	NN
cord-002791-tqchfdmy	76	39	beads	bead	NNS
cord-002791-tqchfdmy	76	40	to	to	TO
cord-002791-tqchfdmy	76	41	access	access	VB
cord-002791-tqchfdmy	76	42	the	the	DT
cord-002791-tqchfdmy	76	43	His	His	NNP
cord-002791-tqchfdmy	76	44	-	-	HYPH
cord-002791-tqchfdmy	76	45	tag	tag	NN
cord-002791-tqchfdmy	76	46	.	.	.
cord-002791-tqchfdmy	77	1	Newly	newly	RB
cord-002791-tqchfdmy	77	2	produced	produce	VBN
cord-002791-tqchfdmy	77	3	single	single	JJ
cord-002791-tqchfdmy	77	4	-	-	HYPH
cord-002791-tqchfdmy	77	5	chain	chain	NN
cord-002791-tqchfdmy	77	6	version	version	NN
cord-002791-tqchfdmy	77	7	coat	coat	NN
cord-002791-tqchfdmy	77	8	protein	protein	NN
cord-002791-tqchfdmy	77	9	dimer	dimer	NNP
cord-002791-tqchfdmy	77	10	containing	contain	VBG
cord-002791-tqchfdmy	77	11	the	the	DT
cord-002791-tqchfdmy	77	12	His	-PRON-	PRP$
cord-002791-tqchfdmy	77	13	-	-	HYPH
cord-002791-tqchfdmy	77	14	tag	tag	NN
cord-002791-tqchfdmy	77	15	was	be	VBD
cord-002791-tqchfdmy	77	16	extensively	extensively	RB
cord-002791-tqchfdmy	77	17	characterized	characterize	VBN
cord-002791-tqchfdmy	77	18	using	use	VBG
cord-002791-tqchfdmy	77	19	SDS	SDS	NNP
cord-002791-tqchfdmy	77	20	-	-	HYPH
cord-002791-tqchfdmy	77	21	PAGE	PAGE	NNP
cord-002791-tqchfdmy	77	22	,	,	,
cord-002791-tqchfdmy	77	23	western	western	JJ
cord-002791-tqchfdmy	77	24	blotting	blotting	NN
cord-002791-tqchfdmy	77	25	and	and	CC
cord-002791-tqchfdmy	77	26	MALDI	MALDI	NNP
cord-002791-tqchfdmy	77	27	-	-	HYPH
cord-002791-tqchfdmy	77	28	TOF	TOF	NNP
cord-002791-tqchfdmy	77	29	analyses	analysis	NNS
cord-002791-tqchfdmy	77	30	.	.	.
cord-002791-tqchfdmy	78	1	The	the	DT
cord-002791-tqchfdmy	78	2	molecular	molecular	JJ
cord-002791-tqchfdmy	78	3	weight	weight	NN
cord-002791-tqchfdmy	78	4	of	of	IN
cord-002791-tqchfdmy	78	5	the	the	DT
cord-002791-tqchfdmy	78	6	wild	wild	JJ
cord-002791-tqchfdmy	78	7	-	-	HYPH
cord-002791-tqchfdmy	78	8	type	type	NN
cord-002791-tqchfdmy	78	9	coat	coat	NN
cord-002791-tqchfdmy	78	10	protein	protein	NN
cord-002791-tqchfdmy	78	11	measured	measure	VBD
cord-002791-tqchfdmy	78	12	using	use	VBG
cord-002791-tqchfdmy	78	13	MALDI	MALDI	NNP
cord-002791-tqchfdmy	78	14	-	-	HYPH
cord-002791-tqchfdmy	78	15	TOF	TOF	NNP
cord-002791-tqchfdmy	78	16	was	be	VBD
cord-002791-tqchfdmy	78	17	13,730	13,730	CD
cord-002791-tqchfdmy	78	18	Da	Da	NNP
cord-002791-tqchfdmy	78	19	,	,	,
cord-002791-tqchfdmy	78	20	which	which	WDT
cord-002791-tqchfdmy	78	21	is	be	VBZ
cord-002791-tqchfdmy	78	22	very	very	RB
cord-002791-tqchfdmy	78	23	close	close	JJ
cord-002791-tqchfdmy	78	24	to	to	IN
cord-002791-tqchfdmy	78	25	its	-PRON-	PRP$
cord-002791-tqchfdmy	78	26	theoretical	theoretical	JJ
cord-002791-tqchfdmy	78	27	value	value	NN
cord-002791-tqchfdmy	78	28	of	of	IN
cord-002791-tqchfdmy	78	29	13,728.54	13,728.54	CD
cord-002791-tqchfdmy	78	30	Da	Da	NNP
cord-002791-tqchfdmy	78	31	.	.	.
cord-002791-tqchfdmy	79	1	The	the	DT
cord-002791-tqchfdmy	79	2	measured	measured	JJ
cord-002791-tqchfdmy	79	3	molecular	molecular	JJ
cord-002791-tqchfdmy	79	4	weight	weight	NN
cord-002791-tqchfdmy	79	5	of	of	IN
cord-002791-tqchfdmy	79	6	the	the	DT
cord-002791-tqchfdmy	79	7	single	single	JJ
cord-002791-tqchfdmy	79	8	-	-	HYPH
cord-002791-tqchfdmy	79	9	chain	chain	NN
cord-002791-tqchfdmy	79	10	version	version	NN
cord-002791-tqchfdmy	79	11	of	of	IN
cord-002791-tqchfdmy	79	12	the	the	DT
cord-002791-tqchfdmy	79	13	coat	coat	NN
cord-002791-tqchfdmy	79	14	protein	protein	NN
cord-002791-tqchfdmy	79	15	dimer	dimer	NN
cord-002791-tqchfdmy	79	16	containing	contain	VBG
cord-002791-tqchfdmy	79	17	the	the	DT
cord-002791-tqchfdmy	79	18	His	-PRON-	PRP$
cord-002791-tqchfdmy	79	19	-	-	HYPH
cord-002791-tqchfdmy	79	20	tag	tag	NN
cord-002791-tqchfdmy	79	21	was	be	VBD
cord-002791-tqchfdmy	79	22	28,329	28,329	CD
cord-002791-tqchfdmy	79	23	Da	Da	NNP
cord-002791-tqchfdmy	79	24	(	(	-LRB-
cord-002791-tqchfdmy	79	25	Fig	Fig	NNP
cord-002791-tqchfdmy	79	26	.	.	.
cord-002791-tqchfdmy	79	27	4E	4E	NNP
cord-002791-tqchfdmy	79	28	)	)	-RRB-
cord-002791-tqchfdmy	79	29	,	,	,
cord-002791-tqchfdmy	79	30	which	which	WDT
cord-002791-tqchfdmy	79	31	is	be	VBZ
cord-002791-tqchfdmy	79	32	also	also	RB
cord-002791-tqchfdmy	79	33	very	very	RB
cord-002791-tqchfdmy	79	34	close	close	JJ
cord-002791-tqchfdmy	79	35	to	to	IN
cord-002791-tqchfdmy	79	36	its	-PRON-	PRP$
cord-002791-tqchfdmy	79	37	theoretical	theoretical	JJ
cord-002791-tqchfdmy	79	38	value	value	NN
cord-002791-tqchfdmy	79	39	of	of	IN
cord-002791-tqchfdmy	79	40	28,333	28,333	CD
cord-002791-tqchfdmy	79	41	Da	Da	NNS
cord-002791-tqchfdmy	79	42	.	.	.
cord-002791-tqchfdmy	80	1	The	the	DT
cord-002791-tqchfdmy	80	2	differences	difference	NNS
cord-002791-tqchfdmy	80	3	between	between	IN
cord-002791-tqchfdmy	80	4	theoretical	theoretical	JJ
cord-002791-tqchfdmy	80	5	and	and	CC
cord-002791-tqchfdmy	80	6	measured	measure	VBN
cord-002791-tqchfdmy	80	7	values	value	NNS
cord-002791-tqchfdmy	80	8	of	of	IN
cord-002791-tqchfdmy	80	9	protein	protein	NN
cord-002791-tqchfdmy	80	10	molecular	molecular	JJ
cord-002791-tqchfdmy	80	11	weights	weight	NNS
cord-002791-tqchfdmy	80	12	are	be	VBP
cord-002791-tqchfdmy	80	13	probably	probably	RB
cord-002791-tqchfdmy	80	14	due	due	JJ
cord-002791-tqchfdmy	80	15	to	to	IN
cord-002791-tqchfdmy	80	16	the	the	DT
cord-002791-tqchfdmy	80	17	sample	sample	NN
cord-002791-tqchfdmy	80	18	preparation	preparation	NN
cord-002791-tqchfdmy	80	19	protocol	protocol	NN
cord-002791-tqchfdmy	80	20	and	and	CC
cord-002791-tqchfdmy	80	21	instrumentation	instrumentation	NN
cord-002791-tqchfdmy	80	22	used	use	VBN
cord-002791-tqchfdmy	80	23	.	.	.
cord-002791-tqchfdmy	81	1	These	these	DT
cord-002791-tqchfdmy	81	2	analyses	analysis	NNS
cord-002791-tqchfdmy	81	3	clearly	clearly	RB
cord-002791-tqchfdmy	81	4	showed	show	VBD
cord-002791-tqchfdmy	81	5	that	that	IN
cord-002791-tqchfdmy	81	6	the	the	DT
cord-002791-tqchfdmy	81	7	desired	desire	VBN
cord-002791-tqchfdmy	81	8	mutagenized	mutagenized	JJ
cord-002791-tqchfdmy	81	9	coat	coat	NN
cord-002791-tqchfdmy	81	10	protein	protein	NN
cord-002791-tqchfdmy	81	11	with	with	IN
cord-002791-tqchfdmy	81	12	the	the	DT
cord-002791-tqchfdmy	81	13	expected	expect	VBN
cord-002791-tqchfdmy	81	14	properties	property	NNS
cord-002791-tqchfdmy	81	15	was	be	VBD
cord-002791-tqchfdmy	81	16	successfully	successfully	RB
cord-002791-tqchfdmy	81	17	obtained	obtain	VBN
cord-002791-tqchfdmy	81	18	after	after	IN
cord-002791-tqchfdmy	81	19	induction	induction	NN
cord-002791-tqchfdmy	81	20	.	.	.
cord-002791-tqchfdmy	82	1	Moreover	moreover	RB
cord-002791-tqchfdmy	82	2	,	,	,
cord-002791-tqchfdmy	82	3	this	this	DT
cord-002791-tqchfdmy	82	4	single	single	JJ
cord-002791-tqchfdmy	82	5	-	-	HYPH
cord-002791-tqchfdmy	82	6	chain	chain	NN
cord-002791-tqchfdmy	82	7	version	version	NN
cord-002791-tqchfdmy	82	8	of	of	IN
cord-002791-tqchfdmy	82	9	the	the	DT
cord-002791-tqchfdmy	82	10	coat	coat	NN
cord-002791-tqchfdmy	82	11	protein	protein	NN
cord-002791-tqchfdmy	82	12	dimer	dimer	NN
cord-002791-tqchfdmy	82	13	containing	contain	VBG
cord-002791-tqchfdmy	82	14	the	the	DT
cord-002791-tqchfdmy	82	15	His	-PRON-	PRP$
cord-002791-tqchfdmy	82	16	-	-	HYPH
cord-002791-tqchfdmy	82	17	tag	tag	NN
cord-002791-tqchfdmy	82	18	was	be	VBD
cord-002791-tqchfdmy	82	19	able	able	JJ
cord-002791-tqchfdmy	82	20	to	to	TO
cord-002791-tqchfdmy	82	21	create	create	VB
cord-002791-tqchfdmy	82	22	compact	compact	JJ
cord-002791-tqchfdmy	82	23	His	-PRON-	PRP$
cord-002791-tqchfdmy	82	24	-	-	HYPH
cord-002791-tqchfdmy	82	25	tagged	tag	VBN
cord-002791-tqchfdmy	82	26	MS2	MS2	NNP
cord-002791-tqchfdmy	82	27	PLP	PLP	NNP
cord-002791-tqchfdmy	82	28	,	,	,
cord-002791-tqchfdmy	82	29	which	which	WDT
cord-002791-tqchfdmy	82	30	were	be	VBD
cord-002791-tqchfdmy	82	31	morphologically	morphologically	RB
cord-002791-tqchfdmy	82	32	indistinguishable	indistinguishable	JJ
cord-002791-tqchfdmy	82	33	from	from	IN
cord-002791-tqchfdmy	82	34	previously	previously	RB
cord-002791-tqchfdmy	82	35	produced	produce	VBN
cord-002791-tqchfdmy	82	36	MS2	MS2	NNP
cord-002791-tqchfdmy	82	37	PLP	PLP	NNP
cord-002791-tqchfdmy	82	38	2	2	CD
cord-002791-tqchfdmy	82	39	,	,	,
cord-002791-tqchfdmy	82	40	8	8	CD
cord-002791-tqchfdmy	82	41	.	.	.
cord-002791-tqchfdmy	83	1	The	the	DT
cord-002791-tqchfdmy	83	2	presence	presence	NN
cord-002791-tqchfdmy	83	3	of	of	IN
cord-002791-tqchfdmy	83	4	the	the	DT
cord-002791-tqchfdmy	83	5	His	His	NNP
cord-002791-tqchfdmy	83	6	-	-	HYPH
cord-002791-tqchfdmy	83	7	tag	tag	NN
cord-002791-tqchfdmy	83	8	in	in	IN
cord-002791-tqchfdmy	83	9	the	the	DT
cord-002791-tqchfdmy	83	10	structure	structure	NN
cord-002791-tqchfdmy	83	11	of	of	IN
cord-002791-tqchfdmy	83	12	the	the	DT
cord-002791-tqchfdmy	83	13	coat	coat	NN
cord-002791-tqchfdmy	83	14	protein	protein	NN
cord-002791-tqchfdmy	83	15	was	be	VBD
cord-002791-tqchfdmy	83	16	used	use	VBN
cord-002791-tqchfdmy	83	17	for	for	IN
cord-002791-tqchfdmy	83	18	purification	purification	NN
cord-002791-tqchfdmy	83	19	of	of	IN
cord-002791-tqchfdmy	83	20	His	-PRON-	PRP$
cord-002791-tqchfdmy	83	21	-	-	HYPH
cord-002791-tqchfdmy	83	22	tagged	tagged	NNP
cord-002791-tqchfdmy	83	23	MS2	MS2	NNP
cord-002791-tqchfdmy	83	24	PLP	PLP	NNP
cord-002791-tqchfdmy	83	25	through	through	IN
cord-002791-tqchfdmy	83	26	FPLC	FPLC	NNP
cord-002791-tqchfdmy	83	27	.	.	.
cord-002791-tqchfdmy	84	1	In	in	IN
cord-002791-tqchfdmy	84	2	comparison	comparison	NN
cord-002791-tqchfdmy	84	3	with	with	IN
cord-002791-tqchfdmy	84	4	the	the	DT
cord-002791-tqchfdmy	84	5	traditionally	traditionally	RB
cord-002791-tqchfdmy	84	6	used	use	VBN
cord-002791-tqchfdmy	84	7	method	method	NN
cord-002791-tqchfdmy	84	8	of	of	IN
cord-002791-tqchfdmy	84	9	ultracentrifugation	ultracentrifugation	NN
cord-002791-tqchfdmy	84	10	4	4	CD
cord-002791-tqchfdmy	84	11	,	,	,
cord-002791-tqchfdmy	84	12	8	8	CD
cord-002791-tqchfdmy	84	13	,	,	,
cord-002791-tqchfdmy	84	14	11	11	CD
cord-002791-tqchfdmy	84	15	,	,	,
cord-002791-tqchfdmy	84	16	the	the	DT
cord-002791-tqchfdmy	84	17	FPLC	FPLC	NNP
cord-002791-tqchfdmy	84	18	approach	approach	NN
cord-002791-tqchfdmy	84	19	is	be	VBZ
cord-002791-tqchfdmy	84	20	a	a	DT
cord-002791-tqchfdmy	84	21	rapid	rapid	JJ
cord-002791-tqchfdmy	84	22	purification	purification	NN
cord-002791-tqchfdmy	84	23	technique	technique	NN
cord-002791-tqchfdmy	84	24	enabling	enable	VBG
cord-002791-tqchfdmy	84	25	the	the	DT
cord-002791-tqchfdmy	84	26	isolation	isolation	NN
cord-002791-tqchfdmy	84	27	of	of	IN
cord-002791-tqchfdmy	84	28	huge	huge	JJ
cord-002791-tqchfdmy	84	29	quantities	quantity	NNS
cord-002791-tqchfdmy	84	30	of	of	IN
cord-002791-tqchfdmy	84	31	His	-PRON-	PRP$
cord-002791-tqchfdmy	84	32	-	-	HYPH
cord-002791-tqchfdmy	84	33	tagged	tag	VBN
cord-002791-tqchfdmy	84	34	MS2	MS2	NNP
cord-002791-tqchfdmy	84	35	PLP	PLP	NNP
cord-002791-tqchfdmy	84	36	(	(	-LRB-
cord-002791-tqchfdmy	84	37	~10	~10	NNP
cord-002791-tqchfdmy	84	38	10	10	CD
cord-002791-tqchfdmy	84	39	particles/µl	particles/µl	NN
cord-002791-tqchfdmy	84	40	;	;	:
cord-002791-tqchfdmy	84	41	RT	RT	NNP
cord-002791-tqchfdmy	84	42	-	-	HYPH
cord-002791-tqchfdmy	84	43	qPCR	qpcr	NN
cord-002791-tqchfdmy	84	44	quantification	quantification	NN
cord-002791-tqchfdmy	84	45	according	accord	VBG
cord-002791-tqchfdmy	84	46	to	to	IN
cord-002791-tqchfdmy	84	47	8	8	CD
cord-002791-tqchfdmy	84	48	)	)	-RRB-
cord-002791-tqchfdmy	84	49	in	in	IN
cord-002791-tqchfdmy	84	50	less	less	JJR
cord-002791-tqchfdmy	84	51	than	than	IN
cord-002791-tqchfdmy	84	52	one	one	CD
cord-002791-tqchfdmy	84	53	hour	hour	NN
cord-002791-tqchfdmy	84	54	.	.	.
cord-002791-tqchfdmy	85	1	In	in	IN
cord-002791-tqchfdmy	85	2	fact	fact	NN
cord-002791-tqchfdmy	85	3	,	,	,
cord-002791-tqchfdmy	85	4	the	the	DT
cord-002791-tqchfdmy	85	5	quantity	quantity	NN
cord-002791-tqchfdmy	85	6	of	of	IN
cord-002791-tqchfdmy	85	7	isolated	isolate	VBN
cord-002791-tqchfdmy	85	8	His	-PRON-	PRP$
cord-002791-tqchfdmy	85	9	-	-	HYPH
cord-002791-tqchfdmy	85	10	tagged	tag	VBN
cord-002791-tqchfdmy	85	11	MS2	MS2	NNP
cord-002791-tqchfdmy	85	12	PLP	PLP	NNP
cord-002791-tqchfdmy	85	13	purified	purify	VBN
cord-002791-tqchfdmy	85	14	through	through	IN
cord-002791-tqchfdmy	85	15	FPLC	FPLC	NNP
cord-002791-tqchfdmy	85	16	is	be	VBZ
cord-002791-tqchfdmy	85	17	one	one	CD
cord-002791-tqchfdmy	85	18	order	order	NN
cord-002791-tqchfdmy	85	19	of	of	IN
cord-002791-tqchfdmy	85	20	magnitude	magnitude	NN
cord-002791-tqchfdmy	85	21	higher	high	JJR
cord-002791-tqchfdmy	85	22	than	than	IN
cord-002791-tqchfdmy	85	23	the	the	DT
cord-002791-tqchfdmy	85	24	quantity	quantity	NN
cord-002791-tqchfdmy	85	25	of	of	IN
cord-002791-tqchfdmy	85	26	MS2	MS2	NNP
cord-002791-tqchfdmy	85	27	PLP	PLP	NNP
cord-002791-tqchfdmy	85	28	purified	purify	VBN
cord-002791-tqchfdmy	85	29	through	through	IN
cord-002791-tqchfdmy	85	30	ultracentrifugation	ultracentrifugation	NN
cord-002791-tqchfdmy	85	31	(	(	-LRB-
cord-002791-tqchfdmy	85	32	~10	~10	NNP
cord-002791-tqchfdmy	85	33	9	9	CD
cord-002791-tqchfdmy	85	34	particles/µl	particles/µl	NN
cord-002791-tqchfdmy	85	35	;	;	:
cord-002791-tqchfdmy	85	36	RT	RT	NNP
cord-002791-tqchfdmy	85	37	-	-	HYPH
cord-002791-tqchfdmy	85	38	qPCR	qpcr	NN
cord-002791-tqchfdmy	85	39	quantification	quantification	NN
cord-002791-tqchfdmy	85	40	according	accord	VBG
cord-002791-tqchfdmy	85	41	to	to	IN
cord-002791-tqchfdmy	85	42	8	8	CD
cord-002791-tqchfdmy	85	43	)	)	-RRB-
cord-002791-tqchfdmy	85	44	.	.	.
cord-002791-tqchfdmy	86	1	The	the	DT
cord-002791-tqchfdmy	86	2	FPLC	FPLC	NNP
cord-002791-tqchfdmy	86	3	purification	purification	NN
cord-002791-tqchfdmy	86	4	procedure	procedure	NN
cord-002791-tqchfdmy	86	5	is	be	VBZ
cord-002791-tqchfdmy	86	6	also	also	RB
cord-002791-tqchfdmy	86	7	not	not	RB
cord-002791-tqchfdmy	86	8	as	as	RB
cord-002791-tqchfdmy	86	9	laborious	laborious	JJ
cord-002791-tqchfdmy	86	10	as	as	IN
cord-002791-tqchfdmy	86	11	traditional	traditional	JJ
cord-002791-tqchfdmy	86	12	methods	method	NNS
cord-002791-tqchfdmy	86	13	of	of	IN
cord-002791-tqchfdmy	86	14	purification	purification	NN
cord-002791-tqchfdmy	86	15	and	and	CC
cord-002791-tqchfdmy	86	16	does	do	VBZ
cord-002791-tqchfdmy	86	17	not	not	RB
cord-002791-tqchfdmy	86	18	have	have	VB
cord-002791-tqchfdmy	86	19	any	any	DT
cord-002791-tqchfdmy	86	20	negative	negative	JJ
cord-002791-tqchfdmy	86	21	impact	impact	NN
cord-002791-tqchfdmy	86	22	on	on	IN
cord-002791-tqchfdmy	86	23	MS2	MS2	NNP
cord-002791-tqchfdmy	86	24	PLP	PLP	NNP
cord-002791-tqchfdmy	86	25	integrity	integrity	NN
cord-002791-tqchfdmy	86	26	.	.	.
cord-002791-tqchfdmy	87	1	The	the	DT
cord-002791-tqchfdmy	87	2	integrity	integrity	NN
cord-002791-tqchfdmy	87	3	of	of	IN
cord-002791-tqchfdmy	87	4	His	-PRON-	PRP$
cord-002791-tqchfdmy	87	5	-	-	HYPH
cord-002791-tqchfdmy	87	6	tagged	tag	VBN
cord-002791-tqchfdmy	87	7	MS2	MS2	NNP
cord-002791-tqchfdmy	87	8	PLP	PLP	NNP
cord-002791-tqchfdmy	87	9	after	after	IN
cord-002791-tqchfdmy	87	10	FPLC	FPLC	NNP
cord-002791-tqchfdmy	87	11	purification	purification	NN
cord-002791-tqchfdmy	87	12	was	be	VBD
cord-002791-tqchfdmy	87	13	tested	test	VBN
cord-002791-tqchfdmy	87	14	by	by	IN
cord-002791-tqchfdmy	87	15	TEM	TEM	NNP
cord-002791-tqchfdmy	87	16	;	;	:
cord-002791-tqchfdmy	87	17	the	the	DT
cord-002791-tqchfdmy	87	18	stability	stability	NN
cord-002791-tqchfdmy	87	19	of	of	IN
cord-002791-tqchfdmy	87	20	particles	particle	NNS
cord-002791-tqchfdmy	87	21	against	against	IN
cord-002791-tqchfdmy	87	22	nucleases	nuclease	NNS
cord-002791-tqchfdmy	87	23	was	be	VBD
cord-002791-tqchfdmy	87	24	also	also	RB
cord-002791-tqchfdmy	87	25	tested	test	VBN
cord-002791-tqchfdmy	87	26	.	.	.
cord-002791-tqchfdmy	88	1	In	in	IN
cord-002791-tqchfdmy	88	2	general	general	JJ
cord-002791-tqchfdmy	88	3	,	,	,
cord-002791-tqchfdmy	88	4	the	the	DT
cord-002791-tqchfdmy	88	5	ability	ability	NN
cord-002791-tqchfdmy	88	6	of	of	IN
cord-002791-tqchfdmy	88	7	MS2	MS2	NNP
cord-002791-tqchfdmy	88	8	PLP	PLP	NNP
cord-002791-tqchfdmy	88	9	to	to	TO
cord-002791-tqchfdmy	88	10	withstand	withstand	VB
cord-002791-tqchfdmy	88	11	the	the	DT
cord-002791-tqchfdmy	88	12	action	action	NN
cord-002791-tqchfdmy	88	13	of	of	IN
cord-002791-tqchfdmy	88	14	nucleases	nuclease	NNS
cord-002791-tqchfdmy	88	15	is	be	VBZ
cord-002791-tqchfdmy	88	16	one	one	CD
cord-002791-tqchfdmy	88	17	of	of	IN
cord-002791-tqchfdmy	88	18	their	-PRON-	PRP$
cord-002791-tqchfdmy	88	19	most	most	RBS
cord-002791-tqchfdmy	88	20	important	important	JJ
cord-002791-tqchfdmy	88	21	properties	property	NNS
cord-002791-tqchfdmy	88	22	,	,	,
cord-002791-tqchfdmy	88	23	because	because	IN
cord-002791-tqchfdmy	88	24	only	only	RB
cord-002791-tqchfdmy	88	25	intact	intact	JJ
cord-002791-tqchfdmy	88	26	particles	particle	NNS
cord-002791-tqchfdmy	88	27	are	be	VBP
cord-002791-tqchfdmy	88	28	able	able	JJ
cord-002791-tqchfdmy	88	29	to	to	TO
cord-002791-tqchfdmy	88	30	protect	protect	VB
cord-002791-tqchfdmy	88	31	the	the	DT
cord-002791-tqchfdmy	88	32	ssRNA	ssrna	NN
cord-002791-tqchfdmy	88	33	that	that	IN
cord-002791-tqchfdmy	88	34	they	-PRON-	PRP
cord-002791-tqchfdmy	88	35	encapsulate	encapsulate	VBP
cord-002791-tqchfdmy	88	36	.	.	.
cord-002791-tqchfdmy	89	1	Testing	test	VBG
cord-002791-tqchfdmy	89	2	the	the	DT
cord-002791-tqchfdmy	89	3	stability	stability	NN
cord-002791-tqchfdmy	89	4	of	of	IN
cord-002791-tqchfdmy	89	5	FPLC	FPLC	NNP
cord-002791-tqchfdmy	89	6	-	-	HYPH
cord-002791-tqchfdmy	89	7	purified	purify	VBN
cord-002791-tqchfdmy	89	8	His	-PRON-	PRP$
cord-002791-tqchfdmy	89	9	-	-	HYPH
cord-002791-tqchfdmy	89	10	tagged	tag	VBN
cord-002791-tqchfdmy	89	11	MS2	MS2	NNP
cord-002791-tqchfdmy	89	12	PLP	PLP	NNP
cord-002791-tqchfdmy	89	13	against	against	IN
cord-002791-tqchfdmy	89	14	nucleases	nuclease	NNS
cord-002791-tqchfdmy	89	15	clearly	clearly	RB
cord-002791-tqchfdmy	89	16	showed	show	VBD
cord-002791-tqchfdmy	89	17	that	that	IN
cord-002791-tqchfdmy	89	18	the	the	DT
cord-002791-tqchfdmy	89	19	particles	particle	NNS
cord-002791-tqchfdmy	89	20	were	be	VBD
cord-002791-tqchfdmy	89	21	able	able	JJ
cord-002791-tqchfdmy	89	22	to	to	TO
cord-002791-tqchfdmy	89	23	effectively	effectively	RB
cord-002791-tqchfdmy	89	24	protect	protect	VB
cord-002791-tqchfdmy	89	25	the	the	DT
cord-002791-tqchfdmy	89	26	ssRNA	ssrna	NN
cord-002791-tqchfdmy	89	27	that	that	IN
cord-002791-tqchfdmy	89	28	they	-PRON-	PRP
cord-002791-tqchfdmy	89	29	encapsidated	encapsidate	VBD
cord-002791-tqchfdmy	89	30	against	against	IN
cord-002791-tqchfdmy	89	31	nuclease	nuclease	NNP
cord-002791-tqchfdmy	89	32	degradation	degradation	NN
cord-002791-tqchfdmy	89	33	.	.	.
cord-002791-tqchfdmy	90	1	It	-PRON-	PRP
cord-002791-tqchfdmy	90	2	was	be	VBD
cord-002791-tqchfdmy	90	3	shown	show	VBN
cord-002791-tqchfdmy	90	4	previously	previously	RB
cord-002791-tqchfdmy	90	5	that	that	IN
cord-002791-tqchfdmy	90	6	MS2	MS2	NNP
cord-002791-tqchfdmy	90	7	PLP	PLP	NNP
cord-002791-tqchfdmy	90	8	consisting	consist	VBG
cord-002791-tqchfdmy	90	9	of	of	IN
cord-002791-tqchfdmy	90	10	a	a	DT
cord-002791-tqchfdmy	90	11	single	single	JJ
cord-002791-tqchfdmy	90	12	-	-	HYPH
cord-002791-tqchfdmy	90	13	chain	chain	NN
cord-002791-tqchfdmy	90	14	version	version	NN
cord-002791-tqchfdmy	90	15	of	of	IN
cord-002791-tqchfdmy	90	16	the	the	DT
cord-002791-tqchfdmy	90	17	coat	coat	NN
cord-002791-tqchfdmy	90	18	protein	protein	NN
cord-002791-tqchfdmy	90	19	dimer	dimer	NNP
cord-002791-tqchfdmy	90	20	have	have	VBP
cord-002791-tqchfdmy	90	21	lower	low	JJR
cord-002791-tqchfdmy	90	22	temperature	temperature	NN
cord-002791-tqchfdmy	90	23	stability	stability	NN
cord-002791-tqchfdmy	90	24	in	in	IN
cord-002791-tqchfdmy	90	25	comparison	comparison	NN
cord-002791-tqchfdmy	90	26	with	with	IN
cord-002791-tqchfdmy	90	27	MS2	MS2	NNP
cord-002791-tqchfdmy	90	28	PLP	PLP	NNP
cord-002791-tqchfdmy	90	29	consisting	consist	VBG
cord-002791-tqchfdmy	90	30	of	of	IN
cord-002791-tqchfdmy	90	31	a	a	DT
cord-002791-tqchfdmy	90	32	wild	wild	JJ
cord-002791-tqchfdmy	90	33	-	-	HYPH
cord-002791-tqchfdmy	90	34	type	type	NN
cord-002791-tqchfdmy	90	35	coat	coat	NN
cord-002791-tqchfdmy	90	36	protein	protein	NN
cord-002791-tqchfdmy	90	37	dimer	dimer	NNP
cord-002791-tqchfdmy	90	38	29	29	CD
cord-002791-tqchfdmy	90	39	,	,	,
cord-002791-tqchfdmy	90	40	30	30	CD
cord-002791-tqchfdmy	90	41	.	.	.
cord-002791-tqchfdmy	91	1	Our	-PRON-	PRP$
cord-002791-tqchfdmy	91	2	results	result	NNS
cord-002791-tqchfdmy	91	3	indicate	indicate	VBP
cord-002791-tqchfdmy	91	4	that	that	IN
cord-002791-tqchfdmy	91	5	lysis	lysis	NN
cord-002791-tqchfdmy	91	6	temperature	temperature	NN
cord-002791-tqchfdmy	91	7	is	be	VBZ
cord-002791-tqchfdmy	91	8	a	a	DT
cord-002791-tqchfdmy	91	9	function	function	NN
cord-002791-tqchfdmy	91	10	of	of	IN
cord-002791-tqchfdmy	91	11	quantity	quantity	NN
cord-002791-tqchfdmy	91	12	,	,	,
cord-002791-tqchfdmy	91	13	time	time	NN
cord-002791-tqchfdmy	91	14	,	,	,
cord-002791-tqchfdmy	91	15	and	and	CC
cord-002791-tqchfdmy	91	16	temperature	temperature	NN
cord-002791-tqchfdmy	91	17	.	.	.
cord-002791-tqchfdmy	92	1	It	-PRON-	PRP
cord-002791-tqchfdmy	92	2	is	be	VBZ
cord-002791-tqchfdmy	92	3	also	also	RB
cord-002791-tqchfdmy	92	4	interesting	interesting	JJ
cord-002791-tqchfdmy	92	5	that	that	IN
cord-002791-tqchfdmy	92	6	the	the	DT
cord-002791-tqchfdmy	92	7	intensity	intensity	NN
cord-002791-tqchfdmy	92	8	of	of	IN
cord-002791-tqchfdmy	92	9	bands	band	NNS
cord-002791-tqchfdmy	92	10	on	on	IN
cord-002791-tqchfdmy	92	11	an	an	DT
cord-002791-tqchfdmy	92	12	agarose	agarose	JJ
cord-002791-tqchfdmy	92	13	gel	gel	NN
cord-002791-tqchfdmy	92	14	was	be	VBD
cord-002791-tqchfdmy	92	15	much	much	RB
cord-002791-tqchfdmy	92	16	higher	high	JJR
cord-002791-tqchfdmy	92	17	in	in	IN
cord-002791-tqchfdmy	92	18	the	the	DT
cord-002791-tqchfdmy	92	19	case	case	NN
cord-002791-tqchfdmy	92	20	of	of	IN
cord-002791-tqchfdmy	92	21	MS2	MS2	NNP
cord-002791-tqchfdmy	92	22	PLP	PLP	NNP
cord-002791-tqchfdmy	92	23	in	in	IN
cord-002791-tqchfdmy	92	24	comparison	comparison	NN
cord-002791-tqchfdmy	92	25	with	with	IN
cord-002791-tqchfdmy	92	26	His	-PRON-	PRP$
cord-002791-tqchfdmy	92	27	-	-	HYPH
cord-002791-tqchfdmy	92	28	tagged	tag	VBN
cord-002791-tqchfdmy	92	29	MS2	MS2	NNP
cord-002791-tqchfdmy	92	30	PLP	PLP	NNP
cord-002791-tqchfdmy	92	31	,	,	,
cord-002791-tqchfdmy	92	32	although	although	IN
cord-002791-tqchfdmy	92	33	the	the	DT
cord-002791-tqchfdmy	92	34	quantity	quantity	NN
cord-002791-tqchfdmy	92	35	of	of	IN
cord-002791-tqchfdmy	92	36	tested	test	VBN
cord-002791-tqchfdmy	92	37	particles	particle	NNS
cord-002791-tqchfdmy	92	38	was	be	VBD
cord-002791-tqchfdmy	92	39	the	the	DT
cord-002791-tqchfdmy	92	40	same-0.7	same-0.7	NNP
cord-002791-tqchfdmy	92	41	µg	µg	XX
cord-002791-tqchfdmy	92	42	per	per	IN
cord-002791-tqchfdmy	92	43	band	band	NN
cord-002791-tqchfdmy	92	44	,	,	,
cord-002791-tqchfdmy	92	45	which	which	WDT
cord-002791-tqchfdmy	92	46	corresponds	correspond	VBZ
cord-002791-tqchfdmy	92	47	approximately	approximately	RB
cord-002791-tqchfdmy	92	48	to	to	IN
cord-002791-tqchfdmy	92	49	10	10	CD
cord-002791-tqchfdmy	92	50	12	12	CD
cord-002791-tqchfdmy	92	51	particles	particle	NNS
cord-002791-tqchfdmy	92	52	8	8	CD
cord-002791-tqchfdmy	92	53	.	.	.
cord-002791-tqchfdmy	93	1	A	a	DT
cord-002791-tqchfdmy	93	2	possible	possible	JJ
cord-002791-tqchfdmy	93	3	explanation	explanation	NN
cord-002791-tqchfdmy	93	4	for	for	IN
cord-002791-tqchfdmy	93	5	this	this	DT
cord-002791-tqchfdmy	93	6	difference	difference	NN
cord-002791-tqchfdmy	93	7	is	be	VBZ
cord-002791-tqchfdmy	93	8	that	that	IN
cord-002791-tqchfdmy	93	9	the	the	DT
cord-002791-tqchfdmy	93	10	lower	low	JJR
cord-002791-tqchfdmy	93	11	intensity	intensity	NN
cord-002791-tqchfdmy	93	12	of	of	IN
cord-002791-tqchfdmy	93	13	His	-PRON-	PRP$
cord-002791-tqchfdmy	93	14	-	-	HYPH
cord-002791-tqchfdmy	93	15	tagged	tag	VBN
cord-002791-tqchfdmy	93	16	MS2	MS2	NNP
cord-002791-tqchfdmy	93	17	PLP	PLP	NNP
cord-002791-tqchfdmy	93	18	bands	band	NNS
cord-002791-tqchfdmy	93	19	is	be	VBZ
cord-002791-tqchfdmy	93	20	related	relate	VBN
cord-002791-tqchfdmy	93	21	to	to	IN
cord-002791-tqchfdmy	93	22	a	a	DT
cord-002791-tqchfdmy	93	23	lower	low	JJR
cord-002791-tqchfdmy	93	24	proportion	proportion	NN
cord-002791-tqchfdmy	93	25	of	of	IN
cord-002791-tqchfdmy	93	26	contaminating	contaminate	VBG
cord-002791-tqchfdmy	93	27	DNA	dna	NN
cord-002791-tqchfdmy	93	28	in	in	IN
cord-002791-tqchfdmy	93	29	comparison	comparison	NN
cord-002791-tqchfdmy	93	30	with	with	IN
cord-002791-tqchfdmy	93	31	MS2	MS2	NNP
cord-002791-tqchfdmy	93	32	PLP	PLP	NNP
cord-002791-tqchfdmy	93	33	(	(	-LRB-
cord-002791-tqchfdmy	93	34	Table	Table	NNP
cord-002791-tqchfdmy	93	35	2	2	CD
cord-002791-tqchfdmy	93	36	)	)	-RRB-
cord-002791-tqchfdmy	93	37	.	.	.
cord-002791-tqchfdmy	94	1	Empirically	empirically	RB
cord-002791-tqchfdmy	94	2	,	,	,
cord-002791-tqchfdmy	94	3	the	the	DT
cord-002791-tqchfdmy	94	4	lysis	lysis	NN
cord-002791-tqchfdmy	94	5	temperature	temperature	NN
cord-002791-tqchfdmy	94	6	of	of	IN
cord-002791-tqchfdmy	94	7	His	-PRON-	PRP$
cord-002791-tqchfdmy	94	8	-	-	HYPH
cord-002791-tqchfdmy	94	9	tagged	tag	VBN
cord-002791-tqchfdmy	94	10	MS2	MS2	NNP
cord-002791-tqchfdmy	94	11	PLP	PLP	NNP
cord-002791-tqchfdmy	94	12	was	be	VBD
cord-002791-tqchfdmy	94	13	determined	determined	JJ
cord-002791-tqchfdmy	94	14	to	to	TO
cord-002791-tqchfdmy	94	15	be	be	VB
cord-002791-tqchfdmy	94	16	62.5	62.5	CD
cord-002791-tqchfdmy	94	17	°	°	NNP
cord-002791-tqchfdmy	94	18	C/5	c/5	NN
cord-002791-tqchfdmy	94	19	min	min	NNP
cord-002791-tqchfdmy	94	20	(	(	-LRB-
cord-002791-tqchfdmy	94	21	Fig	Fig	NNP
cord-002791-tqchfdmy	94	22	.	.	.
cord-002791-tqchfdmy	94	23	6	6	CD
cord-002791-tqchfdmy	94	24	)	)	-RRB-
cord-002791-tqchfdmy	94	25	.	.	.
cord-002791-tqchfdmy	95	1	This	this	DT
cord-002791-tqchfdmy	95	2	feature	feature	NN
cord-002791-tqchfdmy	95	3	was	be	VBD
cord-002791-tqchfdmy	95	4	utilized	utilize	VBN
cord-002791-tqchfdmy	95	5	in	in	IN
cord-002791-tqchfdmy	95	6	our	-PRON-	PRP$
cord-002791-tqchfdmy	95	7	previously	previously	RB
cord-002791-tqchfdmy	95	8	developed	develop	VBN
cord-002791-tqchfdmy	95	9	RT	RT	NNP
cord-002791-tqchfdmy	95	10	-	-	HYPH
cord-002791-tqchfdmy	95	11	qPCR	qpcr	NN
cord-002791-tqchfdmy	95	12	system	system	NN
cord-002791-tqchfdmy	95	13	for	for	IN
cord-002791-tqchfdmy	95	14	quantification	quantification	NN
cord-002791-tqchfdmy	95	15	of	of	IN
cord-002791-tqchfdmy	95	16	MS2	MS2	NNP
cord-002791-tqchfdmy	95	17	PLP	PLP	NNP
cord-002791-tqchfdmy	95	18	,	,	,
cord-002791-tqchfdmy	95	19	which	which	WDT
cord-002791-tqchfdmy	95	20	uses	use	VBZ
cord-002791-tqchfdmy	95	21	a	a	DT
cord-002791-tqchfdmy	95	22	two	two	CD
cord-002791-tqchfdmy	95	23	-	-	HYPH
cord-002791-tqchfdmy	95	24	step	step	NN
cord-002791-tqchfdmy	95	25	format	format	NN
cord-002791-tqchfdmy	95	26	in	in	IN
cord-002791-tqchfdmy	95	27	which	which	WDT
cord-002791-tqchfdmy	95	28	RT	RT	NNP
cord-002791-tqchfdmy	95	29	and	and	CC
cord-002791-tqchfdmy	95	30	qPCR	qPCR	NNP
cord-002791-tqchfdmy	95	31	reactions	reaction	NNS
cord-002791-tqchfdmy	95	32	are	be	VBP
cord-002791-tqchfdmy	95	33	separated	separate	VBN
cord-002791-tqchfdmy	95	34	8	8	CD
cord-002791-tqchfdmy	95	35	.	.	.
cord-002791-tqchfdmy	96	1	The	the	DT
cord-002791-tqchfdmy	96	2	step	step	NN
cord-002791-tqchfdmy	96	3	of	of	IN
cord-002791-tqchfdmy	96	4	particle	particle	NN
cord-002791-tqchfdmy	96	5	lysis	lysis	NN
cord-002791-tqchfdmy	96	6	was	be	VBD
cord-002791-tqchfdmy	96	7	included	include	VBN
cord-002791-tqchfdmy	96	8	in	in	IN
cord-002791-tqchfdmy	96	9	the	the	DT
cord-002791-tqchfdmy	96	10	RT	RT	NNP
cord-002791-tqchfdmy	96	11	protocol	protocol	NN
cord-002791-tqchfdmy	96	12	and	and	CC
cord-002791-tqchfdmy	96	13	it	-PRON-	PRP
cord-002791-tqchfdmy	96	14	was	be	VBD
cord-002791-tqchfdmy	96	15	demonstrated	demonstrate	VBN
cord-002791-tqchfdmy	96	16	that	that	IN
cord-002791-tqchfdmy	96	17	initial	initial	JJ
cord-002791-tqchfdmy	96	18	denaturation	denaturation	NN
cord-002791-tqchfdmy	96	19	of	of	IN
cord-002791-tqchfdmy	96	20	65	65	CD
cord-002791-tqchfdmy	96	21	°	°	NN
cord-002791-tqchfdmy	96	22	C/5	c/5	NN
cord-002791-tqchfdmy	96	23	minutes	minute	NNS
cord-002791-tqchfdmy	96	24	during	during	IN
cord-002791-tqchfdmy	96	25	the	the	DT
cord-002791-tqchfdmy	96	26	RT	RT	NNP
cord-002791-tqchfdmy	96	27	protocol	protocol	NN
cord-002791-tqchfdmy	96	28	is	be	VBZ
cord-002791-tqchfdmy	96	29	sufficient	sufficient	JJ
cord-002791-tqchfdmy	96	30	to	to	TO
cord-002791-tqchfdmy	96	31	lyse	lyse	VB
cord-002791-tqchfdmy	96	32	His	-PRON-	PRP$
cord-002791-tqchfdmy	96	33	-	-	HYPH
cord-002791-tqchfdmy	96	34	tagged	tag	VBN
cord-002791-tqchfdmy	96	35	MS2	MS2	NNP
cord-002791-tqchfdmy	96	36	PLP	PLP	NNP
cord-002791-tqchfdmy	96	37	.	.	.
cord-002791-tqchfdmy	97	1	This	this	DT
cord-002791-tqchfdmy	97	2	approach	approach	NN
cord-002791-tqchfdmy	97	3	not	not	RB
cord-002791-tqchfdmy	97	4	only	only	RB
cord-002791-tqchfdmy	97	5	saves	save	VBZ
cord-002791-tqchfdmy	97	6	time	time	NN
cord-002791-tqchfdmy	97	7	,	,	,
cord-002791-tqchfdmy	97	8	but	but	CC
cord-002791-tqchfdmy	97	9	also	also	RB
cord-002791-tqchfdmy	97	10	represents	represent	VBZ
cord-002791-tqchfdmy	97	11	the	the	DT
cord-002791-tqchfdmy	97	12	gentlest	gentle	JJS
cord-002791-tqchfdmy	97	13	method	method	NN
cord-002791-tqchfdmy	97	14	of	of	IN
cord-002791-tqchfdmy	97	15	thermal	thermal	JJ
cord-002791-tqchfdmy	97	16	lysis	lysis	NN
cord-002791-tqchfdmy	97	17	and	and	CC
cord-002791-tqchfdmy	97	18	provides	provide	VBZ
cord-002791-tqchfdmy	97	19	the	the	DT
cord-002791-tqchfdmy	97	20	highest	high	JJS
cord-002791-tqchfdmy	97	21	RT	rt	NN
cord-002791-tqchfdmy	97	22	-	-	HYPH
cord-002791-tqchfdmy	97	23	qPCR	qpcr	NN
cord-002791-tqchfdmy	97	24	yield	yield	NN
cord-002791-tqchfdmy	97	25	.	.	.
cord-002791-tqchfdmy	98	1	On	on	IN
cord-002791-tqchfdmy	98	2	the	the	DT
cord-002791-tqchfdmy	98	3	other	other	JJ
cord-002791-tqchfdmy	98	4	hand	hand	NN
cord-002791-tqchfdmy	98	5	,	,	,
cord-002791-tqchfdmy	98	6	the	the	DT
cord-002791-tqchfdmy	98	7	results	result	NNS
cord-002791-tqchfdmy	98	8	of	of	IN
cord-002791-tqchfdmy	98	9	this	this	DT
cord-002791-tqchfdmy	98	10	experiment	experiment	NN
cord-002791-tqchfdmy	98	11	also	also	RB
cord-002791-tqchfdmy	98	12	show	show	VBP
cord-002791-tqchfdmy	98	13	that	that	IN
cord-002791-tqchfdmy	98	14	the	the	DT
cord-002791-tqchfdmy	98	15	temperature	temperature	NN
cord-002791-tqchfdmy	98	16	of	of	IN
cord-002791-tqchfdmy	98	17	lysis	lysis	NN
cord-002791-tqchfdmy	98	18	has	have	VBZ
cord-002791-tqchfdmy	98	19	no	no	DT
cord-002791-tqchfdmy	98	20	critical	critical	JJ
cord-002791-tqchfdmy	98	21	effect	effect	NN
cord-002791-tqchfdmy	98	22	on	on	IN
cord-002791-tqchfdmy	98	23	ssRNA	ssrna	NN
cord-002791-tqchfdmy	98	24	stability	stability	NN
cord-002791-tqchfdmy	98	25	,	,	,
cord-002791-tqchfdmy	98	26	and	and	CC
cord-002791-tqchfdmy	98	27	,	,	,
cord-002791-tqchfdmy	98	28	thus	thus	RB
cord-002791-tqchfdmy	98	29	,	,	,
cord-002791-tqchfdmy	98	30	the	the	DT
cord-002791-tqchfdmy	98	31	STE	STE	NNP
cord-002791-tqchfdmy	98	32	buffer	buffer	NN
cord-002791-tqchfdmy	98	33	in	in	IN
cord-002791-tqchfdmy	98	34	which	which	WDT
cord-002791-tqchfdmy	98	35	the	the	DT
cord-002791-tqchfdmy	98	36	particles	particle	NNS
cord-002791-tqchfdmy	98	37	were	be	VBD
cord-002791-tqchfdmy	98	38	diluted	dilute	VBN
cord-002791-tqchfdmy	98	39	and	and	CC
cord-002791-tqchfdmy	98	40	lysed	lyse	VBN
cord-002791-tqchfdmy	98	41	provided	provide	VBN
cord-002791-tqchfdmy	98	42	good	good	JJ
cord-002791-tqchfdmy	98	43	protection	protection	NN
cord-002791-tqchfdmy	98	44	to	to	IN
cord-002791-tqchfdmy	98	45	naked	naked	JJ
cord-002791-tqchfdmy	98	46	ssRNA	ssrna	NN
cord-002791-tqchfdmy	98	47	molecules	molecule	NNS
cord-002791-tqchfdmy	98	48	.	.	.
cord-002791-tqchfdmy	99	1	The	the	DT
cord-002791-tqchfdmy	99	2	purity	purity	NN
cord-002791-tqchfdmy	99	3	of	of	IN
cord-002791-tqchfdmy	99	4	produced	produce	VBN
cord-002791-tqchfdmy	99	5	MS2	MS2	NNP
cord-002791-tqchfdmy	99	6	PLP	PLP	NNP
cord-002791-tqchfdmy	99	7	is	be	VBZ
cord-002791-tqchfdmy	99	8	essential	essential	JJ
cord-002791-tqchfdmy	99	9	,	,	,
cord-002791-tqchfdmy	99	10	and	and	CC
cord-002791-tqchfdmy	99	11	,	,	,
cord-002791-tqchfdmy	99	12	therefore	therefore	RB
cord-002791-tqchfdmy	99	13	,	,	,
cord-002791-tqchfdmy	99	14	elimination	elimination	NN
cord-002791-tqchfdmy	99	15	of	of	IN
cord-002791-tqchfdmy	99	16	contaminating	contaminate	VBG
cord-002791-tqchfdmy	99	17	E.	E.	NNP
cord-002791-tqchfdmy	99	18	coli	coli	NNS
cord-002791-tqchfdmy	99	19	genomic	genomic	JJ
cord-002791-tqchfdmy	99	20	DNA	dna	NN
cord-002791-tqchfdmy	99	21	from	from	IN
cord-002791-tqchfdmy	99	22	lysed	lyse	VBN
cord-002791-tqchfdmy	99	23	bacterial	bacterial	JJ
cord-002791-tqchfdmy	99	24	culture	culture	NN
cord-002791-tqchfdmy	99	25	prior	prior	RB
cord-002791-tqchfdmy	99	26	to	to	IN
cord-002791-tqchfdmy	99	27	MS2	MS2	NNP
cord-002791-tqchfdmy	99	28	PLP	PLP	NNP
cord-002791-tqchfdmy	99	29	purification	purification	NN
cord-002791-tqchfdmy	99	30	is	be	VBZ
cord-002791-tqchfdmy	99	31	a	a	DT
cord-002791-tqchfdmy	99	32	part	part	NN
cord-002791-tqchfdmy	99	33	of	of	IN
cord-002791-tqchfdmy	99	34	each	each	DT
cord-002791-tqchfdmy	99	35	production	production	NN
cord-002791-tqchfdmy	99	36	protocol	protocol	NN
cord-002791-tqchfdmy	99	37	;	;	:
cord-002791-tqchfdmy	99	38	usually	usually	RB
cord-002791-tqchfdmy	99	39	,	,	,
cord-002791-tqchfdmy	99	40	a	a	DT
cord-002791-tqchfdmy	99	41	mixture	mixture	NN
cord-002791-tqchfdmy	99	42	of	of	IN
cord-002791-tqchfdmy	99	43	DNases	dnase	NNS
cord-002791-tqchfdmy	99	44	and	and	CC
cord-002791-tqchfdmy	99	45	RNases	RNases	NNPS
cord-002791-tqchfdmy	99	46	are	be	VBP
cord-002791-tqchfdmy	99	47	used	use	VBN
cord-002791-tqchfdmy	99	48	2	2	CD
cord-002791-tqchfdmy	99	49	,	,	,
cord-002791-tqchfdmy	99	50	3	3	CD
cord-002791-tqchfdmy	99	51	,	,	,
cord-002791-tqchfdmy	99	52	5	5	CD
cord-002791-tqchfdmy	99	53	,	,	,
cord-002791-tqchfdmy	99	54	8	8	CD
cord-002791-tqchfdmy	99	55	,	,	,
cord-002791-tqchfdmy	99	56	11	11	CD
cord-002791-tqchfdmy	99	57	,	,	,
cord-002791-tqchfdmy	99	58	12	12	CD
cord-002791-tqchfdmy	99	59	.	.	.
cord-002791-tqchfdmy	100	1	It	-PRON-	PRP
cord-002791-tqchfdmy	100	2	is	be	VBZ
cord-002791-tqchfdmy	100	3	generally	generally	RB
cord-002791-tqchfdmy	100	4	accepted	accept	VBN
cord-002791-tqchfdmy	100	5	that	that	IN
cord-002791-tqchfdmy	100	6	this	this	DT
cord-002791-tqchfdmy	100	7	treatment	treatment	NN
cord-002791-tqchfdmy	100	8	is	be	VBZ
cord-002791-tqchfdmy	100	9	sufficient	sufficient	JJ
cord-002791-tqchfdmy	100	10	to	to	TO
cord-002791-tqchfdmy	100	11	eliminate	eliminate	VB
cord-002791-tqchfdmy	100	12	contaminating	contaminate	VBG
cord-002791-tqchfdmy	100	13	DNA	dna	NN
cord-002791-tqchfdmy	100	14	.	.	.
cord-002791-tqchfdmy	101	1	However	however	RB
cord-002791-tqchfdmy	101	2	,	,	,
cord-002791-tqchfdmy	101	3	this	this	DT
cord-002791-tqchfdmy	101	4	may	may	MD
cord-002791-tqchfdmy	101	5	not	not	RB
cord-002791-tqchfdmy	101	6	be	be	VB
cord-002791-tqchfdmy	101	7	true	true	JJ
cord-002791-tqchfdmy	101	8	.	.	.
cord-002791-tqchfdmy	102	1	Cheng	Cheng	NNP
cord-002791-tqchfdmy	102	2	et	et	NNP
cord-002791-tqchfdmy	102	3	al	al	NNP
cord-002791-tqchfdmy	102	4	.	.	.
cord-002791-tqchfdmy	103	1	tested	test	VBD
cord-002791-tqchfdmy	103	2	the	the	DT
cord-002791-tqchfdmy	103	3	purity	purity	NN
cord-002791-tqchfdmy	103	4	of	of	IN
cord-002791-tqchfdmy	103	5	MS2	MS2	NNP
cord-002791-tqchfdmy	103	6	PLP	PLP	NNP
cord-002791-tqchfdmy	103	7	purified	purify	VBN
cord-002791-tqchfdmy	103	8	through	through	IN
cord-002791-tqchfdmy	103	9	ultracentrifugation	ultracentrifugation	NN
cord-002791-tqchfdmy	103	10	and	and	CC
cord-002791-tqchfdmy	103	11	found	find	VBD
cord-002791-tqchfdmy	103	12	that	that	IN
cord-002791-tqchfdmy	103	13	they	-PRON-	PRP
cord-002791-tqchfdmy	103	14	exhibit	exhibit	VBP
cord-002791-tqchfdmy	103	15	significant	significant	JJ
cord-002791-tqchfdmy	103	16	DNA	dna	NN
cord-002791-tqchfdmy	103	17	contamination	contamination	NN
cord-002791-tqchfdmy	104	1	13	13	CD
cord-002791-tqchfdmy	104	2	MS2	MS2	NNP
cord-002791-tqchfdmy	104	3	PLP	PLP	NNP
cord-002791-tqchfdmy	104	4	was	be	VBD
cord-002791-tqchfdmy	104	5	only	only	RB
cord-002791-tqchfdmy	104	6	~10	~10	CD
cord-002791-tqchfdmy	104	7	3	3	CD
cord-002791-tqchfdmy	104	8	particles/µl	particles/µl	NNP
cord-002791-tqchfdmy	104	9	)	)	-RRB-
cord-002791-tqchfdmy	104	10	.	.	.
cord-002791-tqchfdmy	105	1	Based	base	VBN
cord-002791-tqchfdmy	105	2	on	on	IN
cord-002791-tqchfdmy	105	3	these	these	DT
cord-002791-tqchfdmy	105	4	findings	finding	NNS
cord-002791-tqchfdmy	105	5	,	,	,
cord-002791-tqchfdmy	105	6	the	the	DT
cord-002791-tqchfdmy	105	7	His	-PRON-	PRP$
cord-002791-tqchfdmy	105	8	-	-	HYPH
cord-002791-tqchfdmy	105	9	tagged	tag	VBN
cord-002791-tqchfdmy	105	10	MS2	MS2	NNP
cord-002791-tqchfdmy	105	11	PLP	PLP	NNP
cord-002791-tqchfdmy	105	12	produced	produce	VBD
cord-002791-tqchfdmy	105	13	in	in	IN
cord-002791-tqchfdmy	105	14	this	this	DT
cord-002791-tqchfdmy	105	15	study	study	NN
cord-002791-tqchfdmy	105	16	and	and	CC
cord-002791-tqchfdmy	105	17	previously	previously	RB
cord-002791-tqchfdmy	105	18	produced	produce	VBD
cord-002791-tqchfdmy	105	19	MS2	MS2	NNP
cord-002791-tqchfdmy	105	20	PLP	PLP	NNP
cord-002791-tqchfdmy	105	21	8	8	CD
cord-002791-tqchfdmy	105	22	were	be	VBD
cord-002791-tqchfdmy	105	23	tested	test	VBN
cord-002791-tqchfdmy	105	24	for	for	IN
cord-002791-tqchfdmy	105	25	the	the	DT
cord-002791-tqchfdmy	105	26	presence	presence	NN
cord-002791-tqchfdmy	105	27	of	of	IN
cord-002791-tqchfdmy	105	28	contaminating	contaminate	VBG
cord-002791-tqchfdmy	105	29	DNA	dna	NN
cord-002791-tqchfdmy	105	30	.	.	.
cord-002791-tqchfdmy	106	1	Because	because	IN
cord-002791-tqchfdmy	106	2	some	some	DT
cord-002791-tqchfdmy	106	3	MS2	MS2	NNP
cord-002791-tqchfdmy	106	4	PLP	PLP	NNP
cord-002791-tqchfdmy	106	5	applications	application	NNS
cord-002791-tqchfdmy	106	6	,	,	,
cord-002791-tqchfdmy	106	7	e.g.	e.g.	RB
cord-002791-tqchfdmy	106	8	,	,	,
cord-002791-tqchfdmy	106	9	use	use	VBP
cord-002791-tqchfdmy	106	10	as	as	IN
cord-002791-tqchfdmy	106	11	a	a	DT
cord-002791-tqchfdmy	106	12	PCV	pcv	NN
cord-002791-tqchfdmy	106	13	in	in	IN
cord-002791-tqchfdmy	106	14	RT	RT	NNP
cord-002791-tqchfdmy	106	15	-	-	HYPH
cord-002791-tqchfdmy	106	16	qPCR	qpcr	NN
cord-002791-tqchfdmy	106	17	detection	detection	NN
cord-002791-tqchfdmy	106	18	and	and	CC
cord-002791-tqchfdmy	106	19	quantification	quantification	NN
cord-002791-tqchfdmy	106	20	of	of	IN
cord-002791-tqchfdmy	106	21	pathogenic	pathogenic	JJ
cord-002791-tqchfdmy	106	22	ssRNA	ssrna	NN
cord-002791-tqchfdmy	106	23	viruses	virus	NNS
cord-002791-tqchfdmy	106	24	from	from	IN
cord-002791-tqchfdmy	106	25	a	a	DT
cord-002791-tqchfdmy	106	26	wide	wide	JJ
cord-002791-tqchfdmy	106	27	range	range	NN
cord-002791-tqchfdmy	106	28	of	of	IN
cord-002791-tqchfdmy	106	29	samples	sample	NNS
cord-002791-tqchfdmy	106	30	,	,	,
cord-002791-tqchfdmy	106	31	require	require	VBP
cord-002791-tqchfdmy	106	32	a	a	DT
cord-002791-tqchfdmy	106	33	greater	great	JJR
cord-002791-tqchfdmy	106	34	quantity	quantity	NN
cord-002791-tqchfdmy	106	35	of	of	IN
cord-002791-tqchfdmy	106	36	MS2	MS2	NNP
cord-002791-tqchfdmy	106	37	PLP	PLP	NNP
cord-002791-tqchfdmy	106	38	(	(	-LRB-
cord-002791-tqchfdmy	106	39	~10	~10	NNP
cord-002791-tqchfdmy	106	40	5	5	CD
cord-002791-tqchfdmy	106	41	-10	-10	SYM
cord-002791-tqchfdmy	106	42	8	8	CD
cord-002791-tqchfdmy	106	43	particles/µl	particles/µl	NNP
cord-002791-tqchfdmy	106	44	)	)	-RRB-
cord-002791-tqchfdmy	106	45	,	,	,
cord-002791-tqchfdmy	106	46	the	the	DT
cord-002791-tqchfdmy	106	47	presence	presence	NN
cord-002791-tqchfdmy	106	48	of	of	IN
cord-002791-tqchfdmy	106	49	contaminating	contaminate	VBG
cord-002791-tqchfdmy	106	50	DNA	DNA	NNP
cord-002791-tqchfdmy	106	51	was	be	VBD
cord-002791-tqchfdmy	106	52	tested	test	VBN
cord-002791-tqchfdmy	106	53	in	in	IN
cord-002791-tqchfdmy	106	54	range	range	NN
cord-002791-tqchfdmy	106	55	of	of	IN
cord-002791-tqchfdmy	106	56	10	10	CD
cord-002791-tqchfdmy	106	57	8	8	CD
cord-002791-tqchfdmy	106	58	-10	-10	CD
cord-002791-tqchfdmy	106	59	5	5	CD
cord-002791-tqchfdmy	107	1	particles/µl	particles/µl	NNP
cord-002791-tqchfdmy	107	2	.	.	.
cord-002791-tqchfdmy	108	1	The	the	DT
cord-002791-tqchfdmy	108	2	results	result	NNS
cord-002791-tqchfdmy	108	3	are	be	VBP
cord-002791-tqchfdmy	108	4	consistent	consistent	JJ
cord-002791-tqchfdmy	108	5	with	with	IN
cord-002791-tqchfdmy	108	6	those	those	DT
cord-002791-tqchfdmy	108	7	reported	report	VBN
cord-002791-tqchfdmy	108	8	previously	previously	RB
cord-002791-tqchfdmy	108	9	13	13	CD
cord-002791-tqchfdmy	108	10	with	with	IN
cord-002791-tqchfdmy	108	11	the	the	DT
cord-002791-tqchfdmy	108	12	difference	difference	NN
cord-002791-tqchfdmy	108	13	that	that	WDT
cord-002791-tqchfdmy	108	14	His	-PRON-	PRP$
cord-002791-tqchfdmy	108	15	-	-	HYPH
cord-002791-tqchfdmy	108	16	tagged	tag	VBN
cord-002791-tqchfdmy	108	17	MS2	MS2	NNP
cord-002791-tqchfdmy	108	18	PLP	PLP	NNP
cord-002791-tqchfdmy	108	19	are	be	VBP
cord-002791-tqchfdmy	108	20	also	also	RB
cord-002791-tqchfdmy	108	21	not	not	RB
cord-002791-tqchfdmy	108	22	absolutely	absolutely	RB
cord-002791-tqchfdmy	108	23	free	free	JJ
cord-002791-tqchfdmy	108	24	of	of	IN
cord-002791-tqchfdmy	108	25	contaminating	contaminate	VBG
cord-002791-tqchfdmy	108	26	DNA	dna	NN
cord-002791-tqchfdmy	108	27	,	,	,
cord-002791-tqchfdmy	108	28	especially	especially	RB
cord-002791-tqchfdmy	108	29	at	at	IN
cord-002791-tqchfdmy	108	30	higher	high	JJR
cord-002791-tqchfdmy	108	31	concentrations	concentration	NNS
cord-002791-tqchfdmy	108	32	.	.	.
cord-002791-tqchfdmy	109	1	On	on	IN
cord-002791-tqchfdmy	109	2	the	the	DT
cord-002791-tqchfdmy	109	3	other	other	JJ
cord-002791-tqchfdmy	109	4	hand	hand	NN
cord-002791-tqchfdmy	109	5	,	,	,
cord-002791-tqchfdmy	109	6	according	accord	VBG
cord-002791-tqchfdmy	109	7	to	to	IN
cord-002791-tqchfdmy	109	8	our	-PRON-	PRP$
cord-002791-tqchfdmy	109	9	findings	finding	NNS
cord-002791-tqchfdmy	110	1	His	-PRON-	PRP$
cord-002791-tqchfdmy	110	2	-	-	HYPH
cord-002791-tqchfdmy	110	3	tagged	tag	VBN
cord-002791-tqchfdmy	110	4	MS2	MS2	NNP
cord-002791-tqchfdmy	110	5	PLP	PLP	NNP
cord-002791-tqchfdmy	110	6	are	be	VBP
cord-002791-tqchfdmy	110	7	always	always	RB
cord-002791-tqchfdmy	110	8	at	at	RB
cord-002791-tqchfdmy	110	9	least	least	JJS
cord-002791-tqchfdmy	110	10	150,000×	150,000×	CD
cord-002791-tqchfdmy	110	11	cleaner	clean	JJR
cord-002791-tqchfdmy	110	12	through	through	IN
cord-002791-tqchfdmy	110	13	all	all	DT
cord-002791-tqchfdmy	110	14	tested	test	VBN
cord-002791-tqchfdmy	110	15	concentrations	concentration	NNS
cord-002791-tqchfdmy	110	16	(	(	-LRB-
cord-002791-tqchfdmy	110	17	10	10	CD
cord-002791-tqchfdmy	110	18	8	8	CD
cord-002791-tqchfdmy	110	19	-10	-10	CD
cord-002791-tqchfdmy	110	20	5	5	CD
cord-002791-tqchfdmy	110	21	particles/µl	particles/µl	NNP
cord-002791-tqchfdmy	110	22	)	)	-RRB-
cord-002791-tqchfdmy	110	23	than	than	IN
cord-002791-tqchfdmy	110	24	MS2	MS2	NNP
cord-002791-tqchfdmy	110	25	PLP	PLP	NNP
cord-002791-tqchfdmy	110	26	purified	purify	VBN
cord-002791-tqchfdmy	110	27	through	through	IN
cord-002791-tqchfdmy	110	28	ultracentrifugation	ultracentrifugation	NN
cord-002791-tqchfdmy	110	29	(	(	-LRB-
cord-002791-tqchfdmy	110	30	Table	table	NN
cord-002791-tqchfdmy	110	31	2	2	CD
cord-002791-tqchfdmy	110	32	)	)	-RRB-
cord-002791-tqchfdmy	110	33	.	.	.
cord-002791-tqchfdmy	111	1	Moreover	moreover	RB
cord-002791-tqchfdmy	111	2	,	,	,
cord-002791-tqchfdmy	111	3	the	the	DT
cord-002791-tqchfdmy	111	4	proportion	proportion	NN
cord-002791-tqchfdmy	111	5	of	of	IN
cord-002791-tqchfdmy	111	6	contaminating	contaminate	VBG
cord-002791-tqchfdmy	111	7	DNA	dna	NN
cord-002791-tqchfdmy	111	8	decreases	decrease	NNS
cord-002791-tqchfdmy	111	9	with	with	IN
cord-002791-tqchfdmy	111	10	progressive	progressive	JJ
cord-002791-tqchfdmy	111	11	dilution	dilution	NN
cord-002791-tqchfdmy	111	12	,	,	,
cord-002791-tqchfdmy	111	13	but	but	CC
cord-002791-tqchfdmy	111	14	only	only	RB
cord-002791-tqchfdmy	111	15	His	-PRON-	PRP$
cord-002791-tqchfdmy	111	16	-	-	HYPH
cord-002791-tqchfdmy	111	17	tagged	tag	VBN
cord-002791-tqchfdmy	111	18	MS2	MS2	NNP
cord-002791-tqchfdmy	111	19	PLP	PLP	NNP
cord-002791-tqchfdmy	111	20	can	can	MD
cord-002791-tqchfdmy	111	21	be	be	VB
cord-002791-tqchfdmy	111	22	diluted	dilute	VBN
cord-002791-tqchfdmy	111	23	to	to	IN
cord-002791-tqchfdmy	111	24	a	a	DT
cord-002791-tqchfdmy	111	25	quantity	quantity	NN
cord-002791-tqchfdmy	111	26	where	where	WRB
cord-002791-tqchfdmy	111	27	contaminating	contaminate	VBG
cord-002791-tqchfdmy	111	28	DNA	dna	NN
cord-002791-tqchfdmy	111	29	is	be	VBZ
cord-002791-tqchfdmy	111	30	not	not	RB
cord-002791-tqchfdmy	111	31	detectable	detectable	JJ
cord-002791-tqchfdmy	111	32	in	in	IN
cord-002791-tqchfdmy	111	33	RT	RT	NNP
cord-002791-tqchfdmy	111	34	-	-	HYPH
cord-002791-tqchfdmy	111	35	qPCR	qPCR	NNP
cord-002791-tqchfdmy	111	36	.	.	.
cord-002791-tqchfdmy	112	1	In	in	IN
cord-002791-tqchfdmy	112	2	general	general	JJ
cord-002791-tqchfdmy	112	3	,	,	,
cord-002791-tqchfdmy	112	4	the	the	DT
cord-002791-tqchfdmy	112	5	purity	purity	NN
cord-002791-tqchfdmy	112	6	of	of	IN
cord-002791-tqchfdmy	112	7	isolated	isolate	VBN
cord-002791-tqchfdmy	112	8	MS2	MS2	NNP
cord-002791-tqchfdmy	112	9	PLP	PLP	NNP
cord-002791-tqchfdmy	112	10	is	be	VBZ
cord-002791-tqchfdmy	112	11	very	very	RB
cord-002791-tqchfdmy	112	12	important	important	JJ
cord-002791-tqchfdmy	112	13	because	because	IN
cord-002791-tqchfdmy	112	14	the	the	DT
cord-002791-tqchfdmy	112	15	presence	presence	NN
cord-002791-tqchfdmy	112	16	of	of	IN
cord-002791-tqchfdmy	112	17	contaminating	contaminate	VBG
cord-002791-tqchfdmy	112	18	DNA	DNA	NNP
cord-002791-tqchfdmy	112	19	could	could	MD
cord-002791-tqchfdmy	112	20	affect	affect	VB
cord-002791-tqchfdmy	112	21	quantification	quantification	NN
cord-002791-tqchfdmy	112	22	in	in	IN
cord-002791-tqchfdmy	112	23	experiments	experiment	NNS
cord-002791-tqchfdmy	112	24	where	where	WRB
cord-002791-tqchfdmy	112	25	particles	particle	NNS
cord-002791-tqchfdmy	112	26	are	be	VBP
cord-002791-tqchfdmy	112	27	used	use	VBN
cord-002791-tqchfdmy	112	28	as	as	IN
cord-002791-tqchfdmy	112	29	a	a	DT
cord-002791-tqchfdmy	112	30	PCV	pcv	NN
cord-002791-tqchfdmy	112	31	,	,	,
cord-002791-tqchfdmy	112	32	or	or	CC
cord-002791-tqchfdmy	112	33	can	can	MD
cord-002791-tqchfdmy	112	34	confer	confer	VB
cord-002791-tqchfdmy	112	35	toxic	toxic	JJ
cord-002791-tqchfdmy	112	36	effects	effect	NNS
cord-002791-tqchfdmy	112	37	on	on	IN
cord-002791-tqchfdmy	112	38	MS2	MS2	NNP
cord-002791-tqchfdmy	112	39	PLP	PLP	NNP
cord-002791-tqchfdmy	112	40	serving	serve	VBG
cord-002791-tqchfdmy	112	41	as	as	IN
cord-002791-tqchfdmy	112	42	vaccines	vaccine	NNS
cord-002791-tqchfdmy	112	43	or	or	CC
cord-002791-tqchfdmy	112	44	drug	drug	NN
cord-002791-tqchfdmy	112	45	delivery	delivery	NN
cord-002791-tqchfdmy	112	46	systems	system	NNS
cord-002791-tqchfdmy	112	47	9	9	CD
cord-002791-tqchfdmy	112	48	.	.	.
cord-002791-tqchfdmy	113	1	Thus	thus	RB
cord-002791-tqchfdmy	113	2	,	,	,
cord-002791-tqchfdmy	113	3	the	the	DT
cord-002791-tqchfdmy	113	4	one	one	CD
cord-002791-tqchfdmy	113	5	-	-	HYPH
cord-002791-tqchfdmy	113	6	plasmid	plasmid	NN
cord-002791-tqchfdmy	113	7	double	double	JJ
cord-002791-tqchfdmy	113	8	-	-	HYPH
cord-002791-tqchfdmy	113	9	expression	expression	NN
cord-002791-tqchfdmy	113	10	His	-PRON-	PRP$
cord-002791-tqchfdmy	113	11	-	-	HYPH
cord-002791-tqchfdmy	113	12	tag	tag	NN
cord-002791-tqchfdmy	113	13	system	system	NN
cord-002791-tqchfdmy	113	14	presented	present	VBN
cord-002791-tqchfdmy	113	15	in	in	IN
cord-002791-tqchfdmy	113	16	this	this	DT
cord-002791-tqchfdmy	113	17	work	work	NN
cord-002791-tqchfdmy	113	18	is	be	VBZ
cord-002791-tqchfdmy	113	19	able	able	JJ
cord-002791-tqchfdmy	113	20	to	to	TO
cord-002791-tqchfdmy	113	21	meet	meet	VB
cord-002791-tqchfdmy	113	22	this	this	DT
cord-002791-tqchfdmy	113	23	demanding	demand	VBG
cord-002791-tqchfdmy	113	24	criterion	criterion	NN
cord-002791-tqchfdmy	113	25	,	,	,
cord-002791-tqchfdmy	113	26	and	and	CC
cord-002791-tqchfdmy	113	27	can	can	MD
cord-002791-tqchfdmy	113	28	be	be	VB
cord-002791-tqchfdmy	113	29	used	use	VBN
cord-002791-tqchfdmy	113	30	for	for	IN
cord-002791-tqchfdmy	113	31	the	the	DT
cord-002791-tqchfdmy	113	32	production	production	NN
cord-002791-tqchfdmy	113	33	of	of	IN
cord-002791-tqchfdmy	113	34	highly	highly	RB
cord-002791-tqchfdmy	113	35	pure	pure	JJ
cord-002791-tqchfdmy	113	36	MS2	MS2	NNP
cord-002791-tqchfdmy	113	37	PLP	PLP	NNP
cord-002791-tqchfdmy	113	38	.	.	.
cord-002791-tqchfdmy	114	1	On	on	IN
cord-002791-tqchfdmy	114	2	the	the	DT
cord-002791-tqchfdmy	114	3	other	other	JJ
cord-002791-tqchfdmy	114	4	hand	hand	NN
cord-002791-tqchfdmy	114	5	,	,	,
cord-002791-tqchfdmy	114	6	the	the	DT
cord-002791-tqchfdmy	114	7	described	describe	VBN
cord-002791-tqchfdmy	114	8	purification	purification	NN
cord-002791-tqchfdmy	114	9	method	method	NN
cord-002791-tqchfdmy	114	10	is	be	VBZ
cord-002791-tqchfdmy	114	11	dedicated	dedicate	VBN
cord-002791-tqchfdmy	114	12	mainly	mainly	RB
cord-002791-tqchfdmy	114	13	for	for	IN
cord-002791-tqchfdmy	114	14	using	use	VBG
cord-002791-tqchfdmy	114	15	MS2	MS2	NNP
cord-002791-tqchfdmy	114	16	PLP	PLP	NNP
cord-002791-tqchfdmy	114	17	as	as	IN
cord-002791-tqchfdmy	114	18	a	a	DT
cord-002791-tqchfdmy	114	19	PCV	pcv	NN
cord-002791-tqchfdmy	114	20	and	and	CC
cord-002791-tqchfdmy	114	21	for	for	IN
cord-002791-tqchfdmy	114	22	using	use	VBG
cord-002791-tqchfdmy	114	23	as	as	IN
cord-002791-tqchfdmy	114	24	a	a	DT
cord-002791-tqchfdmy	114	25	vaccines	vaccine	NNS
cord-002791-tqchfdmy	114	26	or	or	CC
cord-002791-tqchfdmy	114	27	drug	drug	NN
cord-002791-tqchfdmy	114	28	delivery	delivery	NN
cord-002791-tqchfdmy	114	29	systems	system	NNS
cord-002791-tqchfdmy	114	30	it	-PRON-	PRP
cord-002791-tqchfdmy	114	31	is	be	VBZ
cord-002791-tqchfdmy	114	32	needed	need	VBN
cord-002791-tqchfdmy	114	33	to	to	TO
cord-002791-tqchfdmy	114	34	perform	perform	VB
cord-002791-tqchfdmy	114	35	additional	additional	JJ
cord-002791-tqchfdmy	114	36	laboratory	laboratory	NN
cord-002791-tqchfdmy	114	37	tests	test	NNS
cord-002791-tqchfdmy	114	38	.	.	.
cord-002791-tqchfdmy	115	1	Construction	construction	NN
cord-002791-tqchfdmy	115	2	of	of	IN
cord-002791-tqchfdmy	115	3	specific	specific	JJ
cord-002791-tqchfdmy	115	4	control	control	NN
cord-002791-tqchfdmy	115	5	sequence	sequence	NN
cord-002791-tqchfdmy	115	6	.	.	.
cord-002791-tqchfdmy	116	1	A	a	DT
cord-002791-tqchfdmy	116	2	specific	specific	JJ
cord-002791-tqchfdmy	116	3	control	control	NN
cord-002791-tqchfdmy	116	4	sequence	sequence	NN
cord-002791-tqchfdmy	116	5	derived	derive	VBN
cord-002791-tqchfdmy	116	6	from	from	IN
cord-002791-tqchfdmy	116	7	mtDNA	mtdna	JJ
cord-002791-tqchfdmy	116	8	sequences	sequence	NNS
cord-002791-tqchfdmy	116	9	of	of	IN
cord-002791-tqchfdmy	116	10	two	two	CD
cord-002791-tqchfdmy	116	11	extinct	extinct	JJ
cord-002791-tqchfdmy	116	12	species	specie	NNS
cord-002791-tqchfdmy	116	13	-	-	HYPH
cord-002791-tqchfdmy	116	14	thylacine	thylacine	NN
cord-002791-tqchfdmy	116	15	(	(	-LRB-
cord-002791-tqchfdmy	116	16	Thylacinus	Thylacinus	NNP
cord-002791-tqchfdmy	116	17	cynocephalus	cynocephalus	NNP
cord-002791-tqchfdmy	116	18	)	)	-RRB-
cord-002791-tqchfdmy	116	19	and	and	CC
cord-002791-tqchfdmy	116	20	the	the	DT
cord-002791-tqchfdmy	116	21	moa	moa	NNP
cord-002791-tqchfdmy	116	22	bird	bird	NN
cord-002791-tqchfdmy	116	23	(	(	-LRB-
cord-002791-tqchfdmy	116	24	Dinornis	Dinornis	NNP
cord-002791-tqchfdmy	116	25	struthoides)-was	struthoides)-was	XX
cord-002791-tqchfdmy	116	26	constructed	construct	VBN
cord-002791-tqchfdmy	116	27	as	as	IN
cord-002791-tqchfdmy	116	28	described	describe	VBN
cord-002791-tqchfdmy	116	29	previously	previously	RB
cord-002791-tqchfdmy	116	30	8	8	CD
cord-002791-tqchfdmy	116	31	.	.	.
cord-002791-tqchfdmy	117	1	Construction	construction	NN
cord-002791-tqchfdmy	117	2	of	of	IN
cord-002791-tqchfdmy	117	3	pACYCDuet-1-TM	pacycduet-1-tm	CD
cord-002791-tqchfdmy	117	4	.	.	.
cord-002791-tqchfdmy	118	1	The	the	DT
cord-002791-tqchfdmy	118	2	specific	specific	JJ
cord-002791-tqchfdmy	118	3	control	control	NN
cord-002791-tqchfdmy	118	4	sequence	sequence	NN
cord-002791-tqchfdmy	118	5	was	be	VBD
cord-002791-tqchfdmy	118	6	cloned	clone	VBN
cord-002791-tqchfdmy	118	7	into	into	IN
cord-002791-tqchfdmy	118	8	multiple	multiple	JJ
cord-002791-tqchfdmy	118	9	cloning	cloning	NN
cord-002791-tqchfdmy	118	10	site	site	NN
cord-002791-tqchfdmy	118	11	2	2	CD
cord-002791-tqchfdmy	118	12	(	(	-LRB-
cord-002791-tqchfdmy	118	13	MCS2	MCS2	NNP
cord-002791-tqchfdmy	118	14	)	)	-RRB-
cord-002791-tqchfdmy	118	15	of	of	IN
cord-002791-tqchfdmy	118	16	the	the	DT
cord-002791-tqchfdmy	118	17	pACYCDuet-1	pacycduet-1	JJ
cord-002791-tqchfdmy	118	18	vector	vector	NN
cord-002791-tqchfdmy	118	19	(	(	-LRB-
cord-002791-tqchfdmy	118	20	p15A	p15A	NNP
cord-002791-tqchfdmy	118	21	-	-	HYPH
cord-002791-tqchfdmy	118	22	type	type	NN
cord-002791-tqchfdmy	118	23	replication	replication	NN
cord-002791-tqchfdmy	118	24	origin	origin	NN
cord-002791-tqchfdmy	118	25	;	;	:
cord-002791-tqchfdmy	118	26	Novagen	Novagen	NNP
cord-002791-tqchfdmy	118	27	,	,	,
cord-002791-tqchfdmy	118	28	Merck	Merck	NNP
cord-002791-tqchfdmy	118	29	,	,	,
cord-002791-tqchfdmy	118	30	Germany	Germany	NNP
cord-002791-tqchfdmy	118	31	;	;	:
cord-002791-tqchfdmy	118	32	Fig	Fig	NNP
cord-002791-tqchfdmy	118	33	.	.	NNP
cord-002791-tqchfdmy	118	34	1	1	CD
cord-002791-tqchfdmy	118	35	)	)	-RRB-
cord-002791-tqchfdmy	118	36	.	.	.
cord-002791-tqchfdmy	119	1	In	in	IN
cord-002791-tqchfdmy	119	2	brief	brief	NN
cord-002791-tqchfdmy	119	3	,	,	,
cord-002791-tqchfdmy	119	4	a	a	DT
cord-002791-tqchfdmy	119	5	191-bp	191-bp	CD
cord-002791-tqchfdmy	119	6	amplicon	amplicon	NN
cord-002791-tqchfdmy	119	7	encoding	encode	VBG
cord-002791-tqchfdmy	119	8	the	the	DT
cord-002791-tqchfdmy	119	9	specific	specific	JJ
cord-002791-tqchfdmy	119	10	control	control	NN
cord-002791-tqchfdmy	119	11	sequence	sequence	NN
cord-002791-tqchfdmy	119	12	was	be	VBD
cord-002791-tqchfdmy	119	13	obtained	obtain	VBN
cord-002791-tqchfdmy	119	14	by	by	IN
cord-002791-tqchfdmy	119	15	PCR	PCR	NNP
cord-002791-tqchfdmy	119	16	using	use	VBG
cord-002791-tqchfdmy	119	17	a	a	DT
cord-002791-tqchfdmy	119	18	de	de	FW
cord-002791-tqchfdmy	119	19	novo	novo	NN
cord-002791-tqchfdmy	119	20	template	template	NN
cord-002791-tqchfdmy	119	21	construct	construct	NN
cord-002791-tqchfdmy	119	22	and	and	CC
cord-002791-tqchfdmy	119	23	the	the	DT
cord-002791-tqchfdmy	119	24	TM	TM	NNP
cord-002791-tqchfdmy	119	25	-	-	HYPH
cord-002791-tqchfdmy	119	26	NdeI	NdeI	NNP
cord-002791-tqchfdmy	119	27	F	F	NNP
cord-002791-tqchfdmy	119	28	and	and	CC
cord-002791-tqchfdmy	119	29	TM	TM	NNP
cord-002791-tqchfdmy	119	30	-	-	HYPH
cord-002791-tqchfdmy	119	31	AvrII	AvrII	NNP
cord-002791-tqchfdmy	119	32	pac	pac	NNP
cord-002791-tqchfdmy	119	33	R	R	NNP
cord-002791-tqchfdmy	119	34	primer	primer	NN
cord-002791-tqchfdmy	119	35	pair	pair	NN
cord-002791-tqchfdmy	119	36	,	,	,
cord-002791-tqchfdmy	119	37	which	which	WDT
cord-002791-tqchfdmy	119	38	included	include	VBD
cord-002791-tqchfdmy	119	39	NdeI	NdeI	NNP
cord-002791-tqchfdmy	119	40	and	and	CC
cord-002791-tqchfdmy	119	41	AvrII	AvrII	NNP
cord-002791-tqchfdmy	119	42	restriction	restriction	NN
cord-002791-tqchfdmy	119	43	enzyme	enzyme	NN
cord-002791-tqchfdmy	119	44	sites	site	NNS
cord-002791-tqchfdmy	119	45	;	;	:
cord-002791-tqchfdmy	119	46	the	the	DT
cord-002791-tqchfdmy	119	47	TM	TM	NNP
cord-002791-tqchfdmy	119	48	-	-	HYPH
cord-002791-tqchfdmy	119	49	AvrII	AvrII	NNP
cord-002791-tqchfdmy	119	50	pac	pac	NNP
cord-002791-tqchfdmy	119	51	R	R	NNP
cord-002791-tqchfdmy	119	52	primer	primer	NN
cord-002791-tqchfdmy	119	53	also	also	RB
cord-002791-tqchfdmy	119	54	included	include	VBD
cord-002791-tqchfdmy	119	55	one	one	CD
cord-002791-tqchfdmy	119	56	C	c	NN
cord-002791-tqchfdmy	119	57	-	-	HYPH
cord-002791-tqchfdmy	119	58	variant	variant	NN
cord-002791-tqchfdmy	119	59	pac	pac	NNP
cord-002791-tqchfdmy	119	60	site	site	NN
cord-002791-tqchfdmy	119	61	(	(	-LRB-
cord-002791-tqchfdmy	119	62	Table	Table	NNP
cord-002791-tqchfdmy	119	63	3	3	CD
cord-002791-tqchfdmy	119	64	)	)	-RRB-
cord-002791-tqchfdmy	119	65	.	.	.
cord-002791-tqchfdmy	120	1	The	the	DT
cord-002791-tqchfdmy	120	2	reaction	reaction	NN
cord-002791-tqchfdmy	120	3	mixture	mixture	NN
cord-002791-tqchfdmy	120	4	was	be	VBD
cord-002791-tqchfdmy	120	5	composed	compose	VBN
cord-002791-tqchfdmy	120	6	of	of	IN
cord-002791-tqchfdmy	120	7	12.5	12.5	CD
cord-002791-tqchfdmy	120	8	µl	µl	IN
cord-002791-tqchfdmy	120	9	of	of	IN
cord-002791-tqchfdmy	120	10	FastStart	FastStart	NNP
cord-002791-tqchfdmy	120	11	PCR	PCR	NNP
cord-002791-tqchfdmy	120	12	master	master	NN
cord-002791-tqchfdmy	120	13	(	(	-LRB-
cord-002791-tqchfdmy	120	14	Roche	Roche	NNP
cord-002791-tqchfdmy	120	15	Molecular	Molecular	NNP
cord-002791-tqchfdmy	120	16	Diagnostics	Diagnostics	NNPS
cord-002791-tqchfdmy	120	17	,	,	,
cord-002791-tqchfdmy	120	18	Germany	Germany	NNP
cord-002791-tqchfdmy	120	19	)	)	-RRB-
cord-002791-tqchfdmy	120	20	,	,	,
cord-002791-tqchfdmy	120	21	7.5	7.5	CD
cord-002791-tqchfdmy	120	22	pmol	pmol	NN
cord-002791-tqchfdmy	120	23	of	of	IN
cord-002791-tqchfdmy	120	24	each	each	DT
cord-002791-tqchfdmy	120	25	primer	primer	NN
cord-002791-tqchfdmy	120	26	and	and	CC
cord-002791-tqchfdmy	120	27	3.3	3.3	CD
cord-002791-tqchfdmy	120	28	pmol	pmol	NN
cord-002791-tqchfdmy	120	29	of	of	IN
cord-002791-tqchfdmy	120	30	template	template	NN
cord-002791-tqchfdmy	120	31	DNA	dna	NN
cord-002791-tqchfdmy	120	32	.	.	.
cord-002791-tqchfdmy	121	1	The	the	DT
cord-002791-tqchfdmy	121	2	assay	assay	NN
cord-002791-tqchfdmy	121	3	was	be	VBD
cord-002791-tqchfdmy	121	4	run	run	VBN
cord-002791-tqchfdmy	121	5	in	in	IN
cord-002791-tqchfdmy	121	6	a	a	DT
cord-002791-tqchfdmy	121	7	total	total	JJ
cord-002791-tqchfdmy	121	8	volume	volume	NN
cord-002791-tqchfdmy	121	9	of	of	IN
cord-002791-tqchfdmy	121	10	25	25	CD
cord-002791-tqchfdmy	121	11	µl	µl	IN
cord-002791-tqchfdmy	121	12	under	under	IN
cord-002791-tqchfdmy	121	13	the	the	DT
cord-002791-tqchfdmy	121	14	following	follow	VBG
cord-002791-tqchfdmy	121	15	conditions	condition	NNS
cord-002791-tqchfdmy	121	16	:	:	:
cord-002791-tqchfdmy	121	17	95	95	CD
cord-002791-tqchfdmy	121	18	°	°	NN
cord-002791-tqchfdmy	121	19	C	c	NN
cord-002791-tqchfdmy	121	20	for	for	IN
cord-002791-tqchfdmy	121	21	3	3	CD
cord-002791-tqchfdmy	121	22	minutes	minute	NNS
cord-002791-tqchfdmy	121	23	,	,	,
cord-002791-tqchfdmy	121	24	followed	follow	VBN
cord-002791-tqchfdmy	121	25	by	by	IN
cord-002791-tqchfdmy	121	26	25	25	CD
cord-002791-tqchfdmy	121	27	cycles	cycle	NNS
cord-002791-tqchfdmy	121	28	of	of	IN
cord-002791-tqchfdmy	121	29	94	94	CD
cord-002791-tqchfdmy	121	30	°	°	NN
cord-002791-tqchfdmy	121	31	C	c	NN
cord-002791-tqchfdmy	121	32	for	for	IN
cord-002791-tqchfdmy	121	33	30	30	CD
cord-002791-tqchfdmy	121	34	seconds	second	NNS
cord-002791-tqchfdmy	121	35	,	,	,
cord-002791-tqchfdmy	121	36	56	56	CD
cord-002791-tqchfdmy	121	37	°	°	NN
cord-002791-tqchfdmy	121	38	C	c	NN
cord-002791-tqchfdmy	121	39	for	for	IN
cord-002791-tqchfdmy	121	40	30	30	CD
cord-002791-tqchfdmy	121	41	seconds	second	NNS
cord-002791-tqchfdmy	121	42	,	,	,
cord-002791-tqchfdmy	121	43	and	and	CC
cord-002791-tqchfdmy	121	44	72	72	CD
cord-002791-tqchfdmy	121	45	°	°	NN
cord-002791-tqchfdmy	121	46	C	c	NN
cord-002791-tqchfdmy	121	47	for	for	IN
cord-002791-tqchfdmy	121	48	30	30	CD
cord-002791-tqchfdmy	121	49	seconds	second	NNS
cord-002791-tqchfdmy	121	50	;	;	:
cord-002791-tqchfdmy	121	51	final	final	JJ
cord-002791-tqchfdmy	121	52	extension	extension	NN
cord-002791-tqchfdmy	121	53	was	be	VBD
cord-002791-tqchfdmy	121	54	at	at	IN
cord-002791-tqchfdmy	121	55	72	72	CD
cord-002791-tqchfdmy	121	56	°	°	NN
cord-002791-tqchfdmy	121	57	C	c	NN
cord-002791-tqchfdmy	121	58	for	for	IN
cord-002791-tqchfdmy	121	59	5	5	CD
cord-002791-tqchfdmy	121	60	minutes	minute	NNS
cord-002791-tqchfdmy	121	61	.	.	.
cord-002791-tqchfdmy	122	1	The	the	DT
cord-002791-tqchfdmy	122	2	results	result	NNS
cord-002791-tqchfdmy	122	3	of	of	IN
cord-002791-tqchfdmy	122	4	the	the	DT
cord-002791-tqchfdmy	122	5	PCR	PCR	NNP
cord-002791-tqchfdmy	122	6	amplification	amplification	NN
cord-002791-tqchfdmy	122	7	were	be	VBD
cord-002791-tqchfdmy	122	8	examined	examine	VBN
cord-002791-tqchfdmy	122	9	by	by	IN
cord-002791-tqchfdmy	122	10	agarose	agarose	JJ
cord-002791-tqchfdmy	122	11	gel	gel	NN
cord-002791-tqchfdmy	122	12	electrophoresis	electrophoresis	NN
cord-002791-tqchfdmy	122	13	(	(	-LRB-
cord-002791-tqchfdmy	122	14	1	1	CD
cord-002791-tqchfdmy	122	15	%	%	NN
cord-002791-tqchfdmy	122	16	)	)	-RRB-
cord-002791-tqchfdmy	122	17	.	.	.
cord-002791-tqchfdmy	123	1	The	the	DT
cord-002791-tqchfdmy	123	2	PCR	PCR	NNP
cord-002791-tqchfdmy	123	3	product	product	NN
cord-002791-tqchfdmy	123	4	was	be	VBD
cord-002791-tqchfdmy	123	5	purified	purify	VBN
cord-002791-tqchfdmy	123	6	using	use	VBG
cord-002791-tqchfdmy	123	7	the	the	DT
cord-002791-tqchfdmy	123	8	QIAquick	QIAquick	NNP
cord-002791-tqchfdmy	123	9	PCR	PCR	NNP
cord-002791-tqchfdmy	123	10	purification	purification	NN
cord-002791-tqchfdmy	123	11	kit	kit	NN
cord-002791-tqchfdmy	123	12	(	(	-LRB-
cord-002791-tqchfdmy	123	13	Qiagen	Qiagen	NNP
cord-002791-tqchfdmy	123	14	,	,	,
cord-002791-tqchfdmy	123	15	Germany	Germany	NNP
cord-002791-tqchfdmy	123	16	)	)	-RRB-
cord-002791-tqchfdmy	123	17	and	and	CC
cord-002791-tqchfdmy	123	18	subsequently	subsequently	RB
cord-002791-tqchfdmy	123	19	cleaved	cleave	VBD
cord-002791-tqchfdmy	123	20	at	at	IN
cord-002791-tqchfdmy	123	21	37	37	CD
cord-002791-tqchfdmy	123	22	°	°	NN
cord-002791-tqchfdmy	123	23	C	c	NN
cord-002791-tqchfdmy	123	24	for	for	IN
cord-002791-tqchfdmy	123	25	2	2	CD
cord-002791-tqchfdmy	123	26	hours	hour	NNS
cord-002791-tqchfdmy	123	27	.	.	.
cord-002791-tqchfdmy	124	1	The	the	DT
cord-002791-tqchfdmy	124	2	composition	composition	NN
cord-002791-tqchfdmy	124	3	of	of	IN
cord-002791-tqchfdmy	124	4	the	the	DT
cord-002791-tqchfdmy	124	5	restriction	restriction	NN
cord-002791-tqchfdmy	124	6	mixture	mixture	NN
cord-002791-tqchfdmy	124	7	was	be	VBD
cord-002791-tqchfdmy	124	8	500	500	CD
cord-002791-tqchfdmy	124	9	ng	ng	NN
cord-002791-tqchfdmy	124	10	of	of	IN
cord-002791-tqchfdmy	124	11	PCR	PCR	NNP
cord-002791-tqchfdmy	124	12	product	product	NN
cord-002791-tqchfdmy	124	13	,	,	,
cord-002791-tqchfdmy	124	14	2	2	CD
cord-002791-tqchfdmy	124	15	µl	µl	IN
cord-002791-tqchfdmy	124	16	of	of	IN
cord-002791-tqchfdmy	124	17	NEBuffer	NEBuffer	NNP
cord-002791-tqchfdmy	124	18	2	2	CD
cord-002791-tqchfdmy	125	1	(	(	-LRB-
cord-002791-tqchfdmy	125	2	New	New	NNP
cord-002791-tqchfdmy	125	3	England	England	NNP
cord-002791-tqchfdmy	125	4	Biolabs	Biolabs	NNP
cord-002791-tqchfdmy	125	5	,	,	,
cord-002791-tqchfdmy	125	6	UK	UK	NNP
cord-002791-tqchfdmy	125	7	;	;	:
cord-002791-tqchfdmy	125	8	NEB	NEB	NNP
cord-002791-tqchfdmy	125	9	)	)	-RRB-
cord-002791-tqchfdmy	125	10	,	,	,
cord-002791-tqchfdmy	125	11	20	20	CD
cord-002791-tqchfdmy	125	12	U	u	NN
cord-002791-tqchfdmy	125	13	and	and	CC
cord-002791-tqchfdmy	125	14	4	4	CD
cord-002791-tqchfdmy	125	15	U	u	NN
cord-002791-tqchfdmy	125	16	of	of	IN
cord-002791-tqchfdmy	125	17	NdeI	NdeI	NNP
cord-002791-tqchfdmy	125	18	and	and	CC
cord-002791-tqchfdmy	125	19	AvrII	AvrII	NNP
cord-002791-tqchfdmy	125	20	endonucleases	endonuclease	NNS
cord-002791-tqchfdmy	125	21	,	,	,
cord-002791-tqchfdmy	125	22	respectively	respectively	RB
cord-002791-tqchfdmy	125	23	(	(	-LRB-
cord-002791-tqchfdmy	125	24	both	both	DT
cord-002791-tqchfdmy	125	25	NEB	NEB	NNP
cord-002791-tqchfdmy	125	26	)	)	-RRB-
cord-002791-tqchfdmy	125	27	,	,	,
cord-002791-tqchfdmy	125	28	in	in	IN
cord-002791-tqchfdmy	125	29	a	a	DT
cord-002791-tqchfdmy	125	30	final	final	JJ
cord-002791-tqchfdmy	125	31	volume	volume	NN
cord-002791-tqchfdmy	125	32	of	of	IN
cord-002791-tqchfdmy	125	33	20	20	CD
cord-002791-tqchfdmy	125	34	µl	µl	IN
cord-002791-tqchfdmy	125	35	.	.	.
cord-002791-tqchfdmy	126	1	The	the	DT
cord-002791-tqchfdmy	126	2	cleaved	cleaved	JJ
cord-002791-tqchfdmy	126	3	PCR	PCR	NNP
cord-002791-tqchfdmy	126	4	product	product	NN
cord-002791-tqchfdmy	126	5	was	be	VBD
cord-002791-tqchfdmy	126	6	also	also	RB
cord-002791-tqchfdmy	126	7	purified	purify	VBN
cord-002791-tqchfdmy	126	8	using	use	VBG
cord-002791-tqchfdmy	126	9	the	the	DT
cord-002791-tqchfdmy	126	10	QIAquick	QIAquick	NNP
cord-002791-tqchfdmy	126	11	PCR	PCR	NNP
cord-002791-tqchfdmy	126	12	purification	purification	NN
cord-002791-tqchfdmy	126	13	kit	kit	NN
cord-002791-tqchfdmy	126	14	(	(	-LRB-
cord-002791-tqchfdmy	126	15	Qiagen	Qiagen	NNP
cord-002791-tqchfdmy	126	16	)	)	-RRB-
cord-002791-tqchfdmy	126	17	.	.	.
cord-002791-tqchfdmy	127	1	The	the	DT
cord-002791-tqchfdmy	127	2	pACYCDuet-1	pACYCDuet-1	NNP
cord-002791-tqchfdmy	127	3	vector	vector	NN
cord-002791-tqchfdmy	127	4	was	be	VBD
cord-002791-tqchfdmy	127	5	cleaved	cleave	VBN
cord-002791-tqchfdmy	127	6	at	at	IN
cord-002791-tqchfdmy	127	7	37	37	CD
cord-002791-tqchfdmy	127	8	°	°	NN
cord-002791-tqchfdmy	127	9	C	c	NN
cord-002791-tqchfdmy	127	10	for	for	IN
cord-002791-tqchfdmy	127	11	2	2	CD
cord-002791-tqchfdmy	127	12	hours	hour	NNS
cord-002791-tqchfdmy	127	13	.	.	.
cord-002791-tqchfdmy	128	1	The	the	DT
cord-002791-tqchfdmy	128	2	restriction	restriction	NN
cord-002791-tqchfdmy	128	3	mixture	mixture	NN
cord-002791-tqchfdmy	128	4	was	be	VBD
cord-002791-tqchfdmy	128	5	composed	compose	VBN
cord-002791-tqchfdmy	128	6	of	of	IN
cord-002791-tqchfdmy	128	7	500	500	CD
cord-002791-tqchfdmy	128	8	ng	ng	NN
cord-002791-tqchfdmy	128	9	of	of	IN
cord-002791-tqchfdmy	128	10	plasmid	plasmid	NN
cord-002791-tqchfdmy	128	11	DNA	DNA	NNP
cord-002791-tqchfdmy	128	12	,	,	,
cord-002791-tqchfdmy	128	13	5	5	CD
cord-002791-tqchfdmy	128	14	µl	µl	IN
cord-002791-tqchfdmy	128	15	of	of	IN
cord-002791-tqchfdmy	128	16	NEBuffer	NEBuffer	NNP
cord-002791-tqchfdmy	128	17	2	2	CD
cord-002791-tqchfdmy	128	18	(	(	-LRB-
cord-002791-tqchfdmy	128	19	NEB	NEB	NNP
cord-002791-tqchfdmy	128	20	)	)	-RRB-
cord-002791-tqchfdmy	128	21	20	20	CD
cord-002791-tqchfdmy	128	22	U	u	NN
cord-002791-tqchfdmy	128	23	and	and	CC
cord-002791-tqchfdmy	128	24	4	4	CD
cord-002791-tqchfdmy	128	25	U	u	NN
cord-002791-tqchfdmy	128	26	of	of	IN
cord-002791-tqchfdmy	128	27	NdeI	NdeI	NNP
cord-002791-tqchfdmy	128	28	and	and	CC
cord-002791-tqchfdmy	128	29	AvrII	AvrII	NNP
cord-002791-tqchfdmy	128	30	endonucleases	endonuclease	NNS
cord-002791-tqchfdmy	128	31	,	,	,
cord-002791-tqchfdmy	128	32	respectively	respectively	RB
cord-002791-tqchfdmy	128	33	(	(	-LRB-
cord-002791-tqchfdmy	128	34	both	both	DT
cord-002791-tqchfdmy	128	35	NEB	NEB	NNP
cord-002791-tqchfdmy	128	36	)	)	-RRB-
cord-002791-tqchfdmy	128	37	,	,	,
cord-002791-tqchfdmy	128	38	in	in	IN
cord-002791-tqchfdmy	128	39	a	a	DT
cord-002791-tqchfdmy	128	40	final	final	JJ
cord-002791-tqchfdmy	128	41	volume	volume	NN
cord-002791-tqchfdmy	128	42	of	of	IN
cord-002791-tqchfdmy	128	43	50	50	CD
cord-002791-tqchfdmy	128	44	µl	µl	IN
cord-002791-tqchfdmy	128	45	.	.	.
cord-002791-tqchfdmy	129	1	The	the	DT
cord-002791-tqchfdmy	129	2	cleaved	cleaved	JJ
cord-002791-tqchfdmy	129	3	vector	vector	NN
cord-002791-tqchfdmy	129	4	was	be	VBD
cord-002791-tqchfdmy	129	5	dephosphorylated	dephosphorylate	VBN
cord-002791-tqchfdmy	129	6	with	with	IN
cord-002791-tqchfdmy	129	7	0.25	0.25	CD
cord-002791-tqchfdmy	129	8	U	u	NN
cord-002791-tqchfdmy	129	9	of	of	IN
cord-002791-tqchfdmy	129	10	calf	calf	NN
cord-002791-tqchfdmy	129	11	-	-	HYPH
cord-002791-tqchfdmy	129	12	intestinal	intestinal	JJ
cord-002791-tqchfdmy	129	13	alkaline	alkaline	JJ
cord-002791-tqchfdmy	129	14	phosphatase	phosphatase	NN
cord-002791-tqchfdmy	129	15	(	(	-LRB-
cord-002791-tqchfdmy	129	16	CIP	CIP	NNP
cord-002791-tqchfdmy	129	17	)	)	-RRB-
cord-002791-tqchfdmy	129	18	(	(	-LRB-
cord-002791-tqchfdmy	129	19	NEB	NEB	NNP
cord-002791-tqchfdmy	129	20	)	)	-RRB-
cord-002791-tqchfdmy	129	21	at	at	IN
cord-002791-tqchfdmy	129	22	37	37	CD
cord-002791-tqchfdmy	129	23	°	°	NN
cord-002791-tqchfdmy	129	24	C	c	NN
cord-002791-tqchfdmy	129	25	for	for	IN
cord-002791-tqchfdmy	129	26	1	1	CD
cord-002791-tqchfdmy	129	27	hour	hour	NN
cord-002791-tqchfdmy	129	28	.	.	.
cord-002791-tqchfdmy	130	1	Subsequently	subsequently	RB
cord-002791-tqchfdmy	130	2	,	,	,
cord-002791-tqchfdmy	130	3	vector	vector	NN
cord-002791-tqchfdmy	130	4	DNA	DNA	NNP
cord-002791-tqchfdmy	130	5	was	be	VBD
cord-002791-tqchfdmy	130	6	purified	purify	VBN
cord-002791-tqchfdmy	130	7	using	use	VBG
cord-002791-tqchfdmy	130	8	the	the	DT
cord-002791-tqchfdmy	130	9	QIAquick	QIAquick	NNP
cord-002791-tqchfdmy	130	10	PCR	PCR	NNP
cord-002791-tqchfdmy	130	11	purification	purification	NN
cord-002791-tqchfdmy	130	12	kit	kit	NN
cord-002791-tqchfdmy	130	13	(	(	-LRB-
cord-002791-tqchfdmy	130	14	Qiagen	Qiagen	NNP
cord-002791-tqchfdmy	130	15	)	)	-RRB-
cord-002791-tqchfdmy	130	16	.	.	.
cord-002791-tqchfdmy	131	1	Ligation	ligation	NN
cord-002791-tqchfdmy	131	2	of	of	IN
cord-002791-tqchfdmy	131	3	the	the	DT
cord-002791-tqchfdmy	131	4	specific	specific	JJ
cord-002791-tqchfdmy	131	5	control	control	NN
cord-002791-tqchfdmy	131	6	sequence	sequence	NN
cord-002791-tqchfdmy	131	7	to	to	IN
cord-002791-tqchfdmy	131	8	the	the	DT
cord-002791-tqchfdmy	131	9	MCS2	MCS2	NNP
cord-002791-tqchfdmy	131	10	of	of	IN
cord-002791-tqchfdmy	131	11	the	the	DT
cord-002791-tqchfdmy	131	12	cleaved	cleaved	JJ
cord-002791-tqchfdmy	131	13	pACYCDuet-1	pACYCDuet-1	NNP
cord-002791-tqchfdmy	131	14	vector	vector	NN
cord-002791-tqchfdmy	131	15	was	be	VBD
cord-002791-tqchfdmy	131	16	performed	perform	VBN
cord-002791-tqchfdmy	131	17	using	use	VBG
cord-002791-tqchfdmy	131	18	the	the	DT
cord-002791-tqchfdmy	131	19	Quick	quick	JJ
cord-002791-tqchfdmy	131	20	-	-	HYPH
cord-002791-tqchfdmy	131	21	ligation	ligation	NN
cord-002791-tqchfdmy	131	22	kit	kit	NN
cord-002791-tqchfdmy	131	23	(	(	-LRB-
cord-002791-tqchfdmy	131	24	NEB	NEB	NNP
cord-002791-tqchfdmy	131	25	)	)	-RRB-
cord-002791-tqchfdmy	131	26	according	accord	VBG
cord-002791-tqchfdmy	131	27	to	to	IN
cord-002791-tqchfdmy	131	28	the	the	DT
cord-002791-tqchfdmy	131	29	manufacturer	manufacturer	NN
cord-002791-tqchfdmy	131	30	's	's	POS
cord-002791-tqchfdmy	131	31	instructions	instruction	NNS
cord-002791-tqchfdmy	131	32	.	.	.
cord-002791-tqchfdmy	132	1	Transformation	transformation	NN
cord-002791-tqchfdmy	132	2	of	of	IN
cord-002791-tqchfdmy	132	3	E.	E.	NNP
cord-002791-tqchfdmy	132	4	coli	coli	VBZ
cord-002791-tqchfdmy	132	5	TOP10	TOP10	.
cord-002791-tqchfdmy	133	1	(	(	-LRB-
cord-002791-tqchfdmy	133	2	Life	Life	NNP
cord-002791-tqchfdmy	133	3	Technologies	Technologies	NNPS
cord-002791-tqchfdmy	133	4	,	,	,
cord-002791-tqchfdmy	133	5	USA	USA	NNP
cord-002791-tqchfdmy	133	6	)	)	-RRB-
cord-002791-tqchfdmy	133	7	was	be	VBD
cord-002791-tqchfdmy	133	8	also	also	RB
cord-002791-tqchfdmy	133	9	performed	perform	VBN
cord-002791-tqchfdmy	133	10	according	accord	VBG
cord-002791-tqchfdmy	133	11	to	to	IN
cord-002791-tqchfdmy	133	12	the	the	DT
cord-002791-tqchfdmy	133	13	manufacturer	manufacturer	NN
cord-002791-tqchfdmy	133	14	's	's	POS
cord-002791-tqchfdmy	133	15	recommendations	recommendation	NNS
cord-002791-tqchfdmy	133	16	.	.	.
cord-002791-tqchfdmy	134	1	Transformed	transform	VBN
cord-002791-tqchfdmy	134	2	cells	cell	NNS
cord-002791-tqchfdmy	134	3	were	be	VBD
cord-002791-tqchfdmy	134	4	grown	grow	VBN
cord-002791-tqchfdmy	134	5	on	on	IN
cord-002791-tqchfdmy	134	6	Luria	Luria	NNP
cord-002791-tqchfdmy	134	7	-	-	HYPH
cord-002791-tqchfdmy	134	8	Bertani	Bertani	NNP
cord-002791-tqchfdmy	134	9	(	(	-LRB-
cord-002791-tqchfdmy	134	10	LB	LB	NNP
cord-002791-tqchfdmy	134	11	)	)	-RRB-
cord-002791-tqchfdmy	135	1	(	(	-LRB-
cord-002791-tqchfdmy	135	2	Sigma	Sigma	NNP
cord-002791-tqchfdmy	135	3	-	-	HYPH
cord-002791-tqchfdmy	135	4	Aldrich	Aldrich	NNP
cord-002791-tqchfdmy	135	5	,	,	,
cord-002791-tqchfdmy	135	6	Czech	Czech	NNP
cord-002791-tqchfdmy	135	7	Republic	Republic	NNP
cord-002791-tqchfdmy	135	8	)	)	-RRB-
cord-002791-tqchfdmy	135	9	agar	agar	NNP
cord-002791-tqchfdmy	135	10	plates	plate	NNS
cord-002791-tqchfdmy	135	11	containing	contain	VBG
cord-002791-tqchfdmy	135	12	chloramphenicol	chloramphenicol	NNP
cord-002791-tqchfdmy	135	13	(	(	-LRB-
cord-002791-tqchfdmy	135	14	34	34	CD
cord-002791-tqchfdmy	135	15	µg	µg	NN
cord-002791-tqchfdmy	135	16	/	/	SYM
cord-002791-tqchfdmy	135	17	ml	ml	NNP
cord-002791-tqchfdmy	135	18	,	,	,
cord-002791-tqchfdmy	135	19	Sigma	Sigma	NNP
cord-002791-tqchfdmy	135	20	-	-	HYPH
cord-002791-tqchfdmy	135	21	Aldrich	Aldrich	NNP
cord-002791-tqchfdmy	135	22	)	)	-RRB-
cord-002791-tqchfdmy	135	23	overnight	overnight	RB
cord-002791-tqchfdmy	135	24	.	.	.
cord-002791-tqchfdmy	136	1	Plasmid	Plasmid	NNP
cord-002791-tqchfdmy	136	2	DNA	DNA	NNP
cord-002791-tqchfdmy	136	3	was	be	VBD
cord-002791-tqchfdmy	136	4	isolated	isolate	VBN
cord-002791-tqchfdmy	136	5	with	with	IN
cord-002791-tqchfdmy	136	6	the	the	DT
cord-002791-tqchfdmy	136	7	NucleoSpin	NucleoSpin	NNP
cord-002791-tqchfdmy	136	8	Plasmid	Plasmid	NNP
cord-002791-tqchfdmy	136	9	kit	kit	NN
cord-002791-tqchfdmy	136	10	(	(	-LRB-
cord-002791-tqchfdmy	136	11	Macherey	Macherey	NNP
cord-002791-tqchfdmy	136	12	-	-	HYPH
cord-002791-tqchfdmy	136	13	Nagel	Nagel	NNP
cord-002791-tqchfdmy	136	14	,	,	,
cord-002791-tqchfdmy	136	15	Germany	Germany	NNP
cord-002791-tqchfdmy	136	16	)	)	-RRB-
cord-002791-tqchfdmy	136	17	according	accord	VBG
cord-002791-tqchfdmy	136	18	to	to	IN
cord-002791-tqchfdmy	136	19	the	the	DT
cord-002791-tqchfdmy	136	20	manufacturer	manufacturer	NN
cord-002791-tqchfdmy	136	21	's	's	POS
cord-002791-tqchfdmy	136	22	instructions	instruction	NNS
cord-002791-tqchfdmy	136	23	.	.	.
cord-002791-tqchfdmy	137	1	The	the	DT
cord-002791-tqchfdmy	137	2	presence	presence	NN
cord-002791-tqchfdmy	137	3	of	of	IN
cord-002791-tqchfdmy	137	4	specific	specific	JJ
cord-002791-tqchfdmy	137	5	inserts	insert	NNS
cord-002791-tqchfdmy	137	6	was	be	VBD
cord-002791-tqchfdmy	137	7	confirmed	confirm	VBN
cord-002791-tqchfdmy	137	8	by	by	IN
cord-002791-tqchfdmy	137	9	vector	vector	NN
cord-002791-tqchfdmy	137	10	-	-	HYPH
cord-002791-tqchfdmy	137	11	specific	specific	JJ
cord-002791-tqchfdmy	137	12	(	(	-LRB-
cord-002791-tqchfdmy	137	13	DuetUP2	DuetUP2	NNP
cord-002791-tqchfdmy	137	14	and	and	CC
cord-002791-tqchfdmy	137	15	T7	T7	NNP
cord-002791-tqchfdmy	137	16	terminator	terminator	NNP
cord-002791-tqchfdmy	137	17	)	)	-RRB-
cord-002791-tqchfdmy	137	18	and	and	CC
cord-002791-tqchfdmy	137	19	insert	insert	VB
cord-002791-tqchfdmy	137	20	-	-	HYPH
cord-002791-tqchfdmy	137	21	specific	specific	JJ
cord-002791-tqchfdmy	137	22	(	(	-LRB-
cord-002791-tqchfdmy	137	23	TM	TM	NNP
cord-002791-tqchfdmy	137	24	F	F	NNP
cord-002791-tqchfdmy	137	25	and	and	CC
cord-002791-tqchfdmy	137	26	TM	TM	NNP
cord-002791-tqchfdmy	137	27	R	R	NNP
cord-002791-tqchfdmy	137	28	)	)	-RRB-
cord-002791-tqchfdmy	137	29	primers	primer	NNS
cord-002791-tqchfdmy	137	30	(	(	-LRB-
cord-002791-tqchfdmy	137	31	Construction	construction	NN
cord-002791-tqchfdmy	137	32	of	of	IN
cord-002791-tqchfdmy	137	33	the	the	DT
cord-002791-tqchfdmy	137	34	expression	expression	NN
cord-002791-tqchfdmy	137	35	vector	vector	NN
cord-002791-tqchfdmy	137	36	with	with	IN
cord-002791-tqchfdmy	137	37	a	a	DT
cord-002791-tqchfdmy	137	38	single	single	JJ
cord-002791-tqchfdmy	137	39	-	-	HYPH
cord-002791-tqchfdmy	137	40	chain	chain	NN
cord-002791-tqchfdmy	137	41	version	version	NN
cord-002791-tqchfdmy	137	42	of	of	IN
cord-002791-tqchfdmy	137	43	the	the	DT
cord-002791-tqchfdmy	137	44	coat	coat	NN
cord-002791-tqchfdmy	137	45	protein	protein	NN
cord-002791-tqchfdmy	137	46	dimer	dimer	NN
cord-002791-tqchfdmy	137	47	containing	contain	VBG
cord-002791-tqchfdmy	137	48	the	the	DT
cord-002791-tqchfdmy	137	49	His	-PRON-	PRP$
cord-002791-tqchfdmy	137	50	-	-	HYPH
cord-002791-tqchfdmy	137	51	tag	tag	NN
cord-002791-tqchfdmy	137	52	for	for	IN
cord-002791-tqchfdmy	137	53	production	production	NN
cord-002791-tqchfdmy	137	54	of	of	IN
cord-002791-tqchfdmy	137	55	His	-PRON-	PRP$
cord-002791-tqchfdmy	137	56	-	-	HYPH
cord-002791-tqchfdmy	137	57	tagged	tag	VBN
cord-002791-tqchfdmy	137	58	MS2	MS2	NNP
cord-002791-tqchfdmy	137	59	PLP	PLP	NNP
cord-002791-tqchfdmy	137	60	-pACYCDuet-1-TM	-pACYCDuet-1-TM	NNP
cord-002791-tqchfdmy	137	61	-	-	:
cord-002791-tqchfdmy	137	62	CoatDimer	CoatDimer	NNP
cord-002791-tqchfdmy	137	63	-	-	HYPH
cord-002791-tqchfdmy	137	64	His	His	NNP
cord-002791-tqchfdmy	137	65	.	.	.
cord-002791-tqchfdmy	138	1	The	the	DT
cord-002791-tqchfdmy	138	2	sequence	sequence	NN
cord-002791-tqchfdmy	138	3	of	of	IN
cord-002791-tqchfdmy	138	4	maturase	maturase	NN
cord-002791-tqchfdmy	138	5	and	and	CC
cord-002791-tqchfdmy	138	6	the	the	DT
cord-002791-tqchfdmy	138	7	single	single	JJ
cord-002791-tqchfdmy	138	8	-	-	HYPH
cord-002791-tqchfdmy	138	9	chain	chain	NN
cord-002791-tqchfdmy	138	10	version	version	NN
cord-002791-tqchfdmy	138	11	of	of	IN
cord-002791-tqchfdmy	138	12	the	the	DT
cord-002791-tqchfdmy	138	13	coat	coat	NN
cord-002791-tqchfdmy	138	14	protein	protein	NN
cord-002791-tqchfdmy	138	15	dimer	dimer	NN
cord-002791-tqchfdmy	138	16	containing	contain	VBG
cord-002791-tqchfdmy	138	17	the	the	DT
cord-002791-tqchfdmy	138	18	His	-PRON-	PRP$
cord-002791-tqchfdmy	138	19	-	-	HYPH
cord-002791-tqchfdmy	138	20	tag	tag	NN
cord-002791-tqchfdmy	138	21	were	be	VBD
cord-002791-tqchfdmy	138	22	synthesized	synthesize	VBN
cord-002791-tqchfdmy	138	23	de	de	IN
cord-002791-tqchfdmy	138	24	novo	novo	NNP
cord-002791-tqchfdmy	138	25	(	(	-LRB-
cord-002791-tqchfdmy	138	26	Life	Life	NNP
cord-002791-tqchfdmy	138	27	Technologies	Technologies	NNPS
cord-002791-tqchfdmy	138	28	)	)	-RRB-
cord-002791-tqchfdmy	139	1	(	(	-LRB-
cord-002791-tqchfdmy	139	2	Fig	Fig	NNP
cord-002791-tqchfdmy	139	3	.	.	NNP
cord-002791-tqchfdmy	139	4	2	2	CD
cord-002791-tqchfdmy	139	5	)	)	-RRB-
cord-002791-tqchfdmy	139	6	.	.	.
cord-002791-tqchfdmy	140	1	The	the	DT
cord-002791-tqchfdmy	140	2	de	de	FW
cord-002791-tqchfdmy	140	3	novo	novo	NNP
cord-002791-tqchfdmy	140	4	construct	construct	NN
cord-002791-tqchfdmy	140	5	also	also	RB
cord-002791-tqchfdmy	140	6	contained	contain	VBD
cord-002791-tqchfdmy	140	7	a	a	DT
cord-002791-tqchfdmy	140	8	transversion	transversion	NN
cord-002791-tqchfdmy	140	9	of	of	IN
cord-002791-tqchfdmy	140	10	adenine	adenine	NN
cord-002791-tqchfdmy	140	11	(	(	-LRB-
cord-002791-tqchfdmy	140	12	A	a	NN
cord-002791-tqchfdmy	140	13	)	)	-RRB-
cord-002791-tqchfdmy	140	14	at	at	IN
cord-002791-tqchfdmy	140	15	position	position	NN
cord-002791-tqchfdmy	140	16	639	639	CD
cord-002791-tqchfdmy	140	17	to	to	IN
cord-002791-tqchfdmy	140	18	cytosine	cytosine	NN
cord-002791-tqchfdmy	140	19	(	(	-LRB-
cord-002791-tqchfdmy	140	20	C	C	NNP
cord-002791-tqchfdmy	140	21	)	)	-RRB-
cord-002791-tqchfdmy	140	22	.	.	.
cord-002791-tqchfdmy	141	1	This	this	DT
cord-002791-tqchfdmy	141	2	change	change	NN
cord-002791-tqchfdmy	141	3	does	do	VBZ
cord-002791-tqchfdmy	141	4	not	not	RB
cord-002791-tqchfdmy	141	5	influence	influence	VB
cord-002791-tqchfdmy	141	6	the	the	DT
cord-002791-tqchfdmy	141	7	amino	amino	NN
cord-002791-tqchfdmy	141	8	acid	acid	NN
cord-002791-tqchfdmy	141	9	sequence	sequence	NN
cord-002791-tqchfdmy	141	10	of	of	IN
cord-002791-tqchfdmy	141	11	maturase	maturase	NN
cord-002791-tqchfdmy	141	12	but	but	CC
cord-002791-tqchfdmy	141	13	leads	lead	VBZ
cord-002791-tqchfdmy	141	14	to	to	IN
cord-002791-tqchfdmy	141	15	deletion	deletion	NN
cord-002791-tqchfdmy	141	16	of	of	IN
cord-002791-tqchfdmy	141	17	the	the	DT
cord-002791-tqchfdmy	141	18	NdeI	NdeI	NNP
cord-002791-tqchfdmy	141	19	restriction	restriction	NN
cord-002791-tqchfdmy	141	20	site	site	NN
cord-002791-tqchfdmy	141	21	(	(	-LRB-
cord-002791-tqchfdmy	141	22	CATATG	CATATG	NNP
cord-002791-tqchfdmy	141	23	−›	−›	NNP
cord-002791-tqchfdmy	141	24	CCTATG	CCTATG	NNP
cord-002791-tqchfdmy	141	25	)	)	-RRB-
cord-002791-tqchfdmy	141	26	.	.	.
cord-002791-tqchfdmy	142	1	Fusion	fusion	NN
cord-002791-tqchfdmy	142	2	of	of	IN
cord-002791-tqchfdmy	142	3	the	the	DT
cord-002791-tqchfdmy	142	4	two	two	CD
cord-002791-tqchfdmy	142	5	sequences	sequence	NNS
cord-002791-tqchfdmy	142	6	encoding	encode	VBG
cord-002791-tqchfdmy	142	7	the	the	DT
cord-002791-tqchfdmy	142	8	coat	coat	NN
cord-002791-tqchfdmy	142	9	protein	protein	NN
cord-002791-tqchfdmy	142	10	was	be	VBD
cord-002791-tqchfdmy	142	11	done	do	VBN
cord-002791-tqchfdmy	142	12	as	as	IN
cord-002791-tqchfdmy	142	13	in	in	IN
cord-002791-tqchfdmy	142	14	the	the	DT
cord-002791-tqchfdmy	142	15	pCT2dl-13	pct2dl-13	NN
cord-002791-tqchfdmy	142	16	construct	construct	VB
cord-002791-tqchfdmy	142	17	18	18	CD
cord-002791-tqchfdmy	142	18	,	,	,
cord-002791-tqchfdmy	142	19	19	19	CD
cord-002791-tqchfdmy	142	20	.	.	.
cord-002791-tqchfdmy	143	1	The	the	DT
cord-002791-tqchfdmy	143	2	sequence	sequence	NN
cord-002791-tqchfdmy	143	3	(	(	-LRB-
cord-002791-tqchfdmy	143	4	-GGTACCCATCACCATCACCATCACGGTACC-	-ggtacccatcaccatcaccatcacggtacc-	NN
cord-002791-tqchfdmy	143	5	)	)	-RRB-
cord-002791-tqchfdmy	143	6	encoding	encode	VBG
cord-002791-tqchfdmy	143	7	the	the	DT
cord-002791-tqchfdmy	143	8	His	-PRON-	PRP$
cord-002791-tqchfdmy	143	9	-	-	HYPH
cord-002791-tqchfdmy	143	10	tag	tag	NN
cord-002791-tqchfdmy	143	11	flanked	flank	VBN
cord-002791-tqchfdmy	143	12	by	by	IN
cord-002791-tqchfdmy	143	13	two	two	CD
cord-002791-tqchfdmy	143	14	KpnI	KpnI	NNP
cord-002791-tqchfdmy	143	15	restriction	restriction	NN
cord-002791-tqchfdmy	143	16	sites	site	NNS
cord-002791-tqchfdmy	143	17	(	(	-LRB-
cord-002791-tqchfdmy	143	18	underlined	underline	VBN
cord-002791-tqchfdmy	143	19	)	)	-RRB-
cord-002791-tqchfdmy	143	20	13	13	CD
cord-002791-tqchfdmy	143	21	was	be	VBD
cord-002791-tqchfdmy	143	22	inserted	insert	VBN
cord-002791-tqchfdmy	143	23	between	between	IN
cord-002791-tqchfdmy	143	24	residues	residue	NNS
cord-002791-tqchfdmy	143	25	11	11	CD
cord-002791-tqchfdmy	143	26	and	and	CC
cord-002791-tqchfdmy	143	27	12	12	CD
cord-002791-tqchfdmy	143	28	of	of	IN
cord-002791-tqchfdmy	143	29	the	the	DT
cord-002791-tqchfdmy	143	30	second	second	JJ
cord-002791-tqchfdmy	143	31	coat	coat	NN
cord-002791-tqchfdmy	143	32	protein	protein	NN
cord-002791-tqchfdmy	143	33	amino	amino	NN
cord-002791-tqchfdmy	143	34	acid	acid	NN
cord-002791-tqchfdmy	143	35	sequence	sequence	NN
cord-002791-tqchfdmy	143	36	(	(	-LRB-
cord-002791-tqchfdmy	143	37	corresponding	correspond	VBG
cord-002791-tqchfdmy	143	38	to	to	IN
cord-002791-tqchfdmy	143	39	the	the	DT
cord-002791-tqchfdmy	143	40	position	position	NN
cord-002791-tqchfdmy	143	41	between	between	IN
cord-002791-tqchfdmy	143	42	codons	codon	NNS
cord-002791-tqchfdmy	143	43	15	15	CD
cord-002791-tqchfdmy	143	44	and	and	CC
cord-002791-tqchfdmy	143	45	16	16	CD
cord-002791-tqchfdmy	143	46	of	of	IN
cord-002791-tqchfdmy	143	47	wild	wild	JJ
cord-002791-tqchfdmy	143	48	-	-	HYPH
cord-002791-tqchfdmy	143	49	type	type	NN
cord-002791-tqchfdmy	143	50	coat	coat	NN
cord-002791-tqchfdmy	143	51	protein	protein	NN
cord-002791-tqchfdmy	143	52	sequence	sequence	NN
cord-002791-tqchfdmy	143	53	)	)	-RRB-
cord-002791-tqchfdmy	143	54	14	14	CD
cord-002791-tqchfdmy	143	55	.	.	.
cord-002791-tqchfdmy	144	1	The	the	DT
cord-002791-tqchfdmy	144	2	de	de	FW
cord-002791-tqchfdmy	144	3	novo	novo	NNP
cord-002791-tqchfdmy	144	4	construct	construct	NN
cord-002791-tqchfdmy	144	5	was	be	VBD
cord-002791-tqchfdmy	144	6	verified	verify	VBN
cord-002791-tqchfdmy	144	7	by	by	IN
cord-002791-tqchfdmy	144	8	sequencing	sequence	VBG
cord-002791-tqchfdmy	144	9	(	(	-LRB-
cord-002791-tqchfdmy	144	10	Life	Life	NNP
cord-002791-tqchfdmy	144	11	Technologies	Technologies	NNPS
cord-002791-tqchfdmy	144	12	)	)	-RRB-
cord-002791-tqchfdmy	144	13	.	.	.
cord-002791-tqchfdmy	145	1	The	the	DT
cord-002791-tqchfdmy	145	2	fragment	fragment	NN
cord-002791-tqchfdmy	145	3	encoding	encode	VBG
cord-002791-tqchfdmy	145	4	the	the	DT
cord-002791-tqchfdmy	145	5	maturase	maturase	NN
cord-002791-tqchfdmy	145	6	and	and	CC
cord-002791-tqchfdmy	145	7	single	single	JJ
cord-002791-tqchfdmy	145	8	-	-	HYPH
cord-002791-tqchfdmy	145	9	chain	chain	NN
cord-002791-tqchfdmy	145	10	version	version	NN
cord-002791-tqchfdmy	145	11	of	of	IN
cord-002791-tqchfdmy	145	12	the	the	DT
cord-002791-tqchfdmy	145	13	coat	coat	NN
cord-002791-tqchfdmy	145	14	protein	protein	NN
cord-002791-tqchfdmy	145	15	dimer	dimer	NNP
cord-002791-tqchfdmy	145	16	with	with	IN
cord-002791-tqchfdmy	145	17	the	the	DT
cord-002791-tqchfdmy	145	18	His	-PRON-	PRP$
cord-002791-tqchfdmy	145	19	-	-	HYPH
cord-002791-tqchfdmy	145	20	tag	tag	NN
cord-002791-tqchfdmy	145	21	modification	modification	NN
cord-002791-tqchfdmy	145	22	was	be	VBD
cord-002791-tqchfdmy	145	23	obtained	obtain	VBN
cord-002791-tqchfdmy	145	24	by	by	IN
cord-002791-tqchfdmy	145	25	PCR	PCR	NNP
cord-002791-tqchfdmy	145	26	with	with	IN
cord-002791-tqchfdmy	145	27	the	the	DT
cord-002791-tqchfdmy	145	28	KAPA	KAPA	NNP
cord-002791-tqchfdmy	145	29	HiFi	HiFi	NNP
cord-002791-tqchfdmy	145	30	HotStart	HotStart	NNP
cord-002791-tqchfdmy	145	31	PCR	PCR	NNP
cord-002791-tqchfdmy	145	32	Kit	kit	NN
cord-002791-tqchfdmy	145	33	(	(	-LRB-
cord-002791-tqchfdmy	145	34	Kapa	Kapa	NNP
cord-002791-tqchfdmy	145	35	Biosystems	Biosystems	NNP
cord-002791-tqchfdmy	145	36	,	,	,
cord-002791-tqchfdmy	145	37	USA	USA	NNP
cord-002791-tqchfdmy	145	38	)	)	-RRB-
cord-002791-tqchfdmy	145	39	using	use	VBG
cord-002791-tqchfdmy	145	40	the	the	DT
cord-002791-tqchfdmy	145	41	de	de	FW
cord-002791-tqchfdmy	145	42	novo	novo	NNP
cord-002791-tqchfdmy	145	43	template	template	NN
cord-002791-tqchfdmy	145	44	construct	construct	NN
cord-002791-tqchfdmy	145	45	and	and	CC
cord-002791-tqchfdmy	145	46	the	the	DT
cord-002791-tqchfdmy	145	47	MS2	MS2	NNP
cord-002791-tqchfdmy	145	48	NcoI	NcoI	NNP
cord-002791-tqchfdmy	145	49	and	and	CC
cord-002791-tqchfdmy	145	50	MS2	MS2	NNP
cord-002791-tqchfdmy	145	51	NotI	NotI	NNP
cord-002791-tqchfdmy	145	52	primer	primer	NN
cord-002791-tqchfdmy	145	53	pair	pair	NN
cord-002791-tqchfdmy	145	54	,	,	,
cord-002791-tqchfdmy	145	55	which	which	WDT
cord-002791-tqchfdmy	145	56	included	include	VBD
cord-002791-tqchfdmy	145	57	NcoI	NcoI	NNP
cord-002791-tqchfdmy	145	58	and	and	CC
cord-002791-tqchfdmy	145	59	NotI	NotI	NNP
cord-002791-tqchfdmy	145	60	restriction	restriction	NN
cord-002791-tqchfdmy	145	61	enzyme	enzyme	NN
cord-002791-tqchfdmy	145	62	sites	site	NNS
cord-002791-tqchfdmy	145	63	(	(	-LRB-
cord-002791-tqchfdmy	145	64	Table	Table	NNP
cord-002791-tqchfdmy	145	65	1	1	CD
cord-002791-tqchfdmy	145	66	)	)	-RRB-
cord-002791-tqchfdmy	145	67	.	.	.
cord-002791-tqchfdmy	146	1	The	the	DT
cord-002791-tqchfdmy	146	2	reaction	reaction	NN
cord-002791-tqchfdmy	146	3	mixture	mixture	NN
cord-002791-tqchfdmy	146	4	was	be	VBD
cord-002791-tqchfdmy	146	5	composed	compose	VBN
cord-002791-tqchfdmy	146	6	of	of	IN
cord-002791-tqchfdmy	146	7	5	5	CD
cord-002791-tqchfdmy	146	8	µl	µl	IN
cord-002791-tqchfdmy	146	9	of	of	IN
cord-002791-tqchfdmy	146	10	5	5	CD
cord-002791-tqchfdmy	146	11	×	×	NNP
cord-002791-tqchfdmy	146	12	Kapa	Kapa	NNP
cord-002791-tqchfdmy	146	13	HiFi	HiFi	NNP
cord-002791-tqchfdmy	146	14	fidelity	fidelity	NN
cord-002791-tqchfdmy	146	15	buffer	buffer	NN
cord-002791-tqchfdmy	146	16	,	,	,
cord-002791-tqchfdmy	146	17	0.3	0.3	CD
cord-002791-tqchfdmy	146	18	mM	mm	CD
cord-002791-tqchfdmy	146	19	dNTP	dntp	NN
cord-002791-tqchfdmy	146	20	mix	mix	NN
cord-002791-tqchfdmy	146	21	,	,	,
cord-002791-tqchfdmy	146	22	7.5	7.5	CD
cord-002791-tqchfdmy	146	23	pmol	pmol	NN
cord-002791-tqchfdmy	146	24	of	of	IN
cord-002791-tqchfdmy	146	25	each	each	DT
cord-002791-tqchfdmy	146	26	primer	primer	NN
cord-002791-tqchfdmy	146	27	,	,	,
cord-002791-tqchfdmy	146	28	1	1	CD
cord-002791-tqchfdmy	146	29	U	U	NNP
cord-002791-tqchfdmy	146	30	of	of	IN
cord-002791-tqchfdmy	146	31	Kapa	Kapa	NNP
cord-002791-tqchfdmy	146	32	HiFi	HiFi	NNP
cord-002791-tqchfdmy	146	33	polymerase	polymerase	NN
cord-002791-tqchfdmy	146	34	,	,	,
cord-002791-tqchfdmy	146	35	and	and	CC
cord-002791-tqchfdmy	146	36	5	5	CD
cord-002791-tqchfdmy	146	37	pmol	pmol	NN
cord-002791-tqchfdmy	146	38	of	of	IN
cord-002791-tqchfdmy	146	39	template	template	NN
cord-002791-tqchfdmy	146	40	DNA	dna	NN
cord-002791-tqchfdmy	146	41	.	.	.
cord-002791-tqchfdmy	147	1	The	the	DT
cord-002791-tqchfdmy	147	2	assay	assay	NN
cord-002791-tqchfdmy	147	3	was	be	VBD
cord-002791-tqchfdmy	147	4	run	run	VBN
cord-002791-tqchfdmy	147	5	in	in	IN
cord-002791-tqchfdmy	147	6	a	a	DT
cord-002791-tqchfdmy	147	7	total	total	JJ
cord-002791-tqchfdmy	147	8	volume	volume	NN
cord-002791-tqchfdmy	147	9	of	of	IN
cord-002791-tqchfdmy	147	10	25	25	CD
cord-002791-tqchfdmy	147	11	µl	µl	IN
cord-002791-tqchfdmy	147	12	under	under	IN
cord-002791-tqchfdmy	147	13	the	the	DT
cord-002791-tqchfdmy	147	14	following	follow	VBG
cord-002791-tqchfdmy	147	15	conditions	condition	NNS
cord-002791-tqchfdmy	147	16	:	:	:
cord-002791-tqchfdmy	147	17	95	95	CD
cord-002791-tqchfdmy	147	18	°	°	NN
cord-002791-tqchfdmy	147	19	C	c	NN
cord-002791-tqchfdmy	147	20	for	for	IN
cord-002791-tqchfdmy	147	21	5	5	CD
cord-002791-tqchfdmy	147	22	minutes	minute	NNS
cord-002791-tqchfdmy	147	23	,	,	,
cord-002791-tqchfdmy	147	24	followed	follow	VBN
cord-002791-tqchfdmy	147	25	by	by	IN
cord-002791-tqchfdmy	147	26	25	25	CD
cord-002791-tqchfdmy	147	27	cycles	cycle	NNS
cord-002791-tqchfdmy	147	28	of	of	IN
cord-002791-tqchfdmy	147	29	98	98	CD
cord-002791-tqchfdmy	147	30	°	°	NN
cord-002791-tqchfdmy	147	31	C	c	NN
cord-002791-tqchfdmy	147	32	for	for	IN
cord-002791-tqchfdmy	147	33	20	20	CD
cord-002791-tqchfdmy	147	34	seconds	second	NNS
cord-002791-tqchfdmy	147	35	,	,	,
cord-002791-tqchfdmy	147	36	55	55	CD
cord-002791-tqchfdmy	147	37	°	°	NN
cord-002791-tqchfdmy	147	38	C	c	NN
cord-002791-tqchfdmy	147	39	for	for	IN
cord-002791-tqchfdmy	147	40	15	15	CD
cord-002791-tqchfdmy	147	41	seconds	second	NNS
cord-002791-tqchfdmy	147	42	,	,	,
cord-002791-tqchfdmy	147	43	and	and	CC
cord-002791-tqchfdmy	147	44	72	72	CD
cord-002791-tqchfdmy	147	45	°	°	NN
cord-002791-tqchfdmy	147	46	C	c	NN
cord-002791-tqchfdmy	147	47	for	for	IN
cord-002791-tqchfdmy	147	48	70	70	CD
cord-002791-tqchfdmy	147	49	seconds	second	NNS
cord-002791-tqchfdmy	147	50	;	;	:
cord-002791-tqchfdmy	147	51	final	final	JJ
cord-002791-tqchfdmy	147	52	extension	extension	NN
cord-002791-tqchfdmy	147	53	was	be	VBD
cord-002791-tqchfdmy	147	54	at	at	IN
cord-002791-tqchfdmy	147	55	72	72	CD
cord-002791-tqchfdmy	147	56	°	°	NN
cord-002791-tqchfdmy	147	57	C	c	NN
cord-002791-tqchfdmy	147	58	for	for	IN
cord-002791-tqchfdmy	147	59	5	5	CD
cord-002791-tqchfdmy	147	60	minutes	minute	NNS
cord-002791-tqchfdmy	147	61	.	.	.
cord-002791-tqchfdmy	148	1	The	the	DT
cord-002791-tqchfdmy	148	2	results	result	NNS
cord-002791-tqchfdmy	148	3	of	of	IN
cord-002791-tqchfdmy	148	4	the	the	DT
cord-002791-tqchfdmy	148	5	PCR	PCR	NNP
cord-002791-tqchfdmy	148	6	amplification	amplification	NN
cord-002791-tqchfdmy	148	7	were	be	VBD
cord-002791-tqchfdmy	148	8	examined	examine	VBN
cord-002791-tqchfdmy	148	9	by	by	IN
cord-002791-tqchfdmy	148	10	agarose	agarose	JJ
cord-002791-tqchfdmy	148	11	gel	gel	NN
cord-002791-tqchfdmy	148	12	electrophoresis	electrophoresis	NN
cord-002791-tqchfdmy	148	13	(	(	-LRB-
cord-002791-tqchfdmy	148	14	1	1	CD
cord-002791-tqchfdmy	148	15	%	%	NN
cord-002791-tqchfdmy	148	16	)	)	-RRB-
cord-002791-tqchfdmy	148	17	.	.	.
cord-002791-tqchfdmy	149	1	The	the	DT
cord-002791-tqchfdmy	149	2	PCR	PCR	NNP
cord-002791-tqchfdmy	149	3	product	product	NN
cord-002791-tqchfdmy	149	4	was	be	VBD
cord-002791-tqchfdmy	149	5	purified	purify	VBN
cord-002791-tqchfdmy	149	6	using	use	VBG
cord-002791-tqchfdmy	149	7	the	the	DT
cord-002791-tqchfdmy	149	8	QIAquick	QIAquick	NNP
cord-002791-tqchfdmy	149	9	PCR	PCR	NNP
cord-002791-tqchfdmy	149	10	purification	purification	NN
cord-002791-tqchfdmy	149	11	kit	kit	NN
cord-002791-tqchfdmy	149	12	(	(	-LRB-
cord-002791-tqchfdmy	149	13	Qiagen	Qiagen	NNP
cord-002791-tqchfdmy	149	14	)	)	-RRB-
cord-002791-tqchfdmy	149	15	and	and	CC
cord-002791-tqchfdmy	149	16	subsequently	subsequently	RB
cord-002791-tqchfdmy	149	17	cleaved	cleave	VBD
cord-002791-tqchfdmy	149	18	at	at	IN
cord-002791-tqchfdmy	149	19	37	37	CD
cord-002791-tqchfdmy	149	20	°	°	NN
cord-002791-tqchfdmy	149	21	C	c	NN
cord-002791-tqchfdmy	149	22	for	for	IN
cord-002791-tqchfdmy	149	23	2	2	CD
cord-002791-tqchfdmy	149	24	hours	hour	NNS
cord-002791-tqchfdmy	149	25	.	.	.
cord-002791-tqchfdmy	150	1	The	the	DT
cord-002791-tqchfdmy	150	2	restriction	restriction	NN
cord-002791-tqchfdmy	150	3	mixture	mixture	NN
cord-002791-tqchfdmy	150	4	was	be	VBD
cord-002791-tqchfdmy	150	5	composed	compose	VBN
cord-002791-tqchfdmy	150	6	of	of	IN
cord-002791-tqchfdmy	150	7	500	500	CD
cord-002791-tqchfdmy	150	8	ng	ng	NN
cord-002791-tqchfdmy	150	9	of	of	IN
cord-002791-tqchfdmy	150	10	PCR	PCR	NNP
cord-002791-tqchfdmy	150	11	product	product	NN
cord-002791-tqchfdmy	150	12	,	,	,
cord-002791-tqchfdmy	150	13	2	2	CD
cord-002791-tqchfdmy	150	14	µl	µl	IN
cord-002791-tqchfdmy	150	15	of	of	IN
cord-002791-tqchfdmy	150	16	NEBuffer	NEBuffer	NNP
cord-002791-tqchfdmy	150	17	3	3	CD
cord-002791-tqchfdmy	150	18	(	(	-LRB-
cord-002791-tqchfdmy	150	19	NEB	NEB	NNP
cord-002791-tqchfdmy	150	20	)	)	-RRB-
cord-002791-tqchfdmy	150	21	,	,	,
cord-002791-tqchfdmy	150	22	and	and	CC
cord-002791-tqchfdmy	150	23	10	10	CD
cord-002791-tqchfdmy	150	24	U	u	NN
cord-002791-tqchfdmy	150	25	each	each	DT
cord-002791-tqchfdmy	150	26	of	of	IN
cord-002791-tqchfdmy	150	27	NcoI	NcoI	NNP
cord-002791-tqchfdmy	150	28	and	and	CC
cord-002791-tqchfdmy	150	29	NotI	NotI	NNP
cord-002791-tqchfdmy	150	30	endonucleases	endonuclease	NNS
cord-002791-tqchfdmy	150	31	(	(	-LRB-
cord-002791-tqchfdmy	150	32	both	both	DT
cord-002791-tqchfdmy	150	33	NEB	NEB	NNP
cord-002791-tqchfdmy	150	34	)	)	-RRB-
cord-002791-tqchfdmy	150	35	,	,	,
cord-002791-tqchfdmy	150	36	in	in	IN
cord-002791-tqchfdmy	150	37	a	a	DT
cord-002791-tqchfdmy	150	38	final	final	JJ
cord-002791-tqchfdmy	150	39	volume	volume	NN
cord-002791-tqchfdmy	150	40	of	of	IN
cord-002791-tqchfdmy	150	41	20	20	CD
cord-002791-tqchfdmy	150	42	µl	µl	IN
cord-002791-tqchfdmy	150	43	.	.	.
cord-002791-tqchfdmy	151	1	The	the	DT
cord-002791-tqchfdmy	151	2	cleaved	cleaved	JJ
cord-002791-tqchfdmy	151	3	PCR	PCR	NNP
cord-002791-tqchfdmy	151	4	product	product	NN
cord-002791-tqchfdmy	151	5	was	be	VBD
cord-002791-tqchfdmy	151	6	purified	purify	VBN
cord-002791-tqchfdmy	151	7	using	use	VBG
cord-002791-tqchfdmy	151	8	the	the	DT
cord-002791-tqchfdmy	151	9	QIAquick	QIAquick	NNP
cord-002791-tqchfdmy	151	10	PCR	PCR	NNP
cord-002791-tqchfdmy	151	11	purification	purification	NN
cord-002791-tqchfdmy	151	12	kit	kit	NN
cord-002791-tqchfdmy	151	13	(	(	-LRB-
cord-002791-tqchfdmy	151	14	Qiagen	Qiagen	NNP
cord-002791-tqchfdmy	151	15	)	)	-RRB-
cord-002791-tqchfdmy	151	16	.	.	.
cord-002791-tqchfdmy	152	1	The	the	DT
cord-002791-tqchfdmy	152	2	pACYCDuet-1-TM	pacycduet-1-tm	CD
cord-002791-tqchfdmy	152	3	vector	vector	NN
cord-002791-tqchfdmy	152	4	was	be	VBD
cord-002791-tqchfdmy	152	5	cleaved	cleave	VBN
cord-002791-tqchfdmy	152	6	at	at	IN
cord-002791-tqchfdmy	152	7	37	37	CD
cord-002791-tqchfdmy	152	8	°	°	NN
cord-002791-tqchfdmy	152	9	C	c	NN
cord-002791-tqchfdmy	152	10	for	for	IN
cord-002791-tqchfdmy	152	11	2	2	CD
cord-002791-tqchfdmy	152	12	hours	hour	NNS
cord-002791-tqchfdmy	152	13	.	.	.
cord-002791-tqchfdmy	153	1	The	the	DT
cord-002791-tqchfdmy	153	2	restriction	restriction	NN
cord-002791-tqchfdmy	153	3	mixture	mixture	NN
cord-002791-tqchfdmy	153	4	was	be	VBD
cord-002791-tqchfdmy	153	5	composed	compose	VBN
cord-002791-tqchfdmy	153	6	of	of	IN
cord-002791-tqchfdmy	153	7	500	500	CD
cord-002791-tqchfdmy	153	8	ng	ng	NN
cord-002791-tqchfdmy	153	9	of	of	IN
cord-002791-tqchfdmy	153	10	plasmid	plasmid	NN
cord-002791-tqchfdmy	153	11	DNA	DNA	NNP
cord-002791-tqchfdmy	153	12	,	,	,
cord-002791-tqchfdmy	153	13	5	5	CD
cord-002791-tqchfdmy	153	14	µl	µl	IN
cord-002791-tqchfdmy	153	15	of	of	IN
cord-002791-tqchfdmy	153	16	NEBuffer	NEBuffer	NNP
cord-002791-tqchfdmy	153	17	3	3	CD
cord-002791-tqchfdmy	153	18	(	(	-LRB-
cord-002791-tqchfdmy	153	19	NEB	NEB	NNP
cord-002791-tqchfdmy	153	20	)	)	-RRB-
cord-002791-tqchfdmy	153	21	,	,	,
cord-002791-tqchfdmy	153	22	and	and	CC
cord-002791-tqchfdmy	153	23	10	10	CD
cord-002791-tqchfdmy	153	24	U	u	NN
cord-002791-tqchfdmy	153	25	each	each	DT
cord-002791-tqchfdmy	153	26	of	of	IN
cord-002791-tqchfdmy	153	27	NcoI	NcoI	NNP
cord-002791-tqchfdmy	153	28	and	and	CC
cord-002791-tqchfdmy	153	29	NotI	NotI	NNP
cord-002791-tqchfdmy	153	30	endonucleases	endonuclease	NNS
cord-002791-tqchfdmy	153	31	(	(	-LRB-
cord-002791-tqchfdmy	153	32	both	both	DT
cord-002791-tqchfdmy	153	33	NEB	NEB	NNP
cord-002791-tqchfdmy	153	34	)	)	-RRB-
cord-002791-tqchfdmy	153	35	,	,	,
cord-002791-tqchfdmy	153	36	in	in	IN
cord-002791-tqchfdmy	153	37	a	a	DT
cord-002791-tqchfdmy	153	38	final	final	JJ
cord-002791-tqchfdmy	153	39	volume	volume	NN
cord-002791-tqchfdmy	153	40	of	of	IN
cord-002791-tqchfdmy	153	41	50	50	CD
cord-002791-tqchfdmy	153	42	µl	µl	IN
cord-002791-tqchfdmy	153	43	.	.	.
cord-002791-tqchfdmy	154	1	Cleaved	cleaved	JJ
cord-002791-tqchfdmy	154	2	vector	vector	NN
cord-002791-tqchfdmy	154	3	was	be	VBD
cord-002791-tqchfdmy	154	4	dephosphorylated	dephosphorylate	VBN
cord-002791-tqchfdmy	154	5	using	use	VBG
cord-002791-tqchfdmy	154	6	0.25	0.25	CD
cord-002791-tqchfdmy	154	7	U	U	NNP
cord-002791-tqchfdmy	154	8	CIP	CIP	NNP
cord-002791-tqchfdmy	154	9	(	(	-LRB-
cord-002791-tqchfdmy	154	10	NEB	NEB	NNP
cord-002791-tqchfdmy	154	11	)	)	-RRB-
cord-002791-tqchfdmy	154	12	at	at	IN
cord-002791-tqchfdmy	154	13	37	37	CD
cord-002791-tqchfdmy	154	14	°	°	NN
cord-002791-tqchfdmy	154	15	C	c	NN
cord-002791-tqchfdmy	154	16	for	for	IN
cord-002791-tqchfdmy	154	17	1	1	CD
cord-002791-tqchfdmy	154	18	hour	hour	NN
cord-002791-tqchfdmy	154	19	.	.	.
cord-002791-tqchfdmy	155	1	Subsequently	subsequently	RB
cord-002791-tqchfdmy	155	2	,	,	,
cord-002791-tqchfdmy	155	3	vector	vector	NN
cord-002791-tqchfdmy	155	4	DNA	DNA	NNP
cord-002791-tqchfdmy	155	5	was	be	VBD
cord-002791-tqchfdmy	155	6	purified	purify	VBN
cord-002791-tqchfdmy	155	7	using	use	VBG
cord-002791-tqchfdmy	155	8	the	the	DT
cord-002791-tqchfdmy	155	9	QIAquick	QIAquick	NNP
cord-002791-tqchfdmy	155	10	PCR	PCR	NNP
cord-002791-tqchfdmy	155	11	purification	purification	NN
cord-002791-tqchfdmy	155	12	kit	kit	NN
cord-002791-tqchfdmy	155	13	(	(	-LRB-
cord-002791-tqchfdmy	155	14	Qiagen	Qiagen	NNP
cord-002791-tqchfdmy	155	15	)	)	-RRB-
cord-002791-tqchfdmy	155	16	.	.	.
cord-002791-tqchfdmy	156	1	Ligation	ligation	NN
cord-002791-tqchfdmy	156	2	of	of	IN
cord-002791-tqchfdmy	156	3	the	the	DT
cord-002791-tqchfdmy	156	4	fragment	fragment	NN
cord-002791-tqchfdmy	156	5	encoding	encode	VBG
cord-002791-tqchfdmy	156	6	the	the	DT
cord-002791-tqchfdmy	156	7	maturase	maturase	NN
cord-002791-tqchfdmy	156	8	and	and	CC
cord-002791-tqchfdmy	156	9	single	single	JJ
cord-002791-tqchfdmy	156	10	-	-	HYPH
cord-002791-tqchfdmy	156	11	chain	chain	NN
cord-002791-tqchfdmy	156	12	version	version	NN
cord-002791-tqchfdmy	156	13	of	of	IN
cord-002791-tqchfdmy	156	14	the	the	DT
cord-002791-tqchfdmy	156	15	coat	coat	NN
cord-002791-tqchfdmy	156	16	protein	protein	NN
cord-002791-tqchfdmy	156	17	dimer	dimer	NNP
cord-002791-tqchfdmy	156	18	with	with	IN
cord-002791-tqchfdmy	156	19	the	the	DT
cord-002791-tqchfdmy	156	20	His	-PRON-	PRP$
cord-002791-tqchfdmy	156	21	-	-	HYPH
cord-002791-tqchfdmy	156	22	tag	tag	NN
cord-002791-tqchfdmy	156	23	modification	modification	NN
cord-002791-tqchfdmy	156	24	to	to	IN
cord-002791-tqchfdmy	156	25	the	the	DT
cord-002791-tqchfdmy	156	26	MCS1	MCS1	NNP
cord-002791-tqchfdmy	156	27	of	of	IN
cord-002791-tqchfdmy	156	28	the	the	DT
cord-002791-tqchfdmy	156	29	pACYCDuet-1-TM	pacycduet-1-tm	CD
cord-002791-tqchfdmy	156	30	vector	vector	NN
cord-002791-tqchfdmy	156	31	was	be	VBD
cord-002791-tqchfdmy	156	32	performed	perform	VBN
cord-002791-tqchfdmy	156	33	using	use	VBG
cord-002791-tqchfdmy	156	34	the	the	DT
cord-002791-tqchfdmy	156	35	Quick	quick	JJ
cord-002791-tqchfdmy	156	36	-	-	HYPH
cord-002791-tqchfdmy	156	37	ligation	ligation	NN
cord-002791-tqchfdmy	156	38	kit	kit	NN
cord-002791-tqchfdmy	156	39	(	(	-LRB-
cord-002791-tqchfdmy	156	40	NEB	NEB	NNP
cord-002791-tqchfdmy	156	41	)	)	-RRB-
cord-002791-tqchfdmy	156	42	according	accord	VBG
cord-002791-tqchfdmy	156	43	to	to	IN
cord-002791-tqchfdmy	156	44	the	the	DT
cord-002791-tqchfdmy	156	45	manufacturer	manufacturer	NN
cord-002791-tqchfdmy	156	46	's	's	POS
cord-002791-tqchfdmy	156	47	instructions	instruction	NNS
cord-002791-tqchfdmy	156	48	.	.	.
cord-002791-tqchfdmy	157	1	Transformation	transformation	NN
cord-002791-tqchfdmy	157	2	of	of	IN
cord-002791-tqchfdmy	157	3	E.	E.	NNP
cord-002791-tqchfdmy	157	4	coli	coli	VBZ
cord-002791-tqchfdmy	157	5	TOP10	TOP10	.
cord-002791-tqchfdmy	158	1	(	(	-LRB-
cord-002791-tqchfdmy	158	2	Life	Life	NNP
cord-002791-tqchfdmy	158	3	Technologies	Technologies	NNPS
cord-002791-tqchfdmy	158	4	)	)	-RRB-
cord-002791-tqchfdmy	158	5	was	be	VBD
cord-002791-tqchfdmy	158	6	also	also	RB
cord-002791-tqchfdmy	158	7	performed	perform	VBN
cord-002791-tqchfdmy	158	8	according	accord	VBG
cord-002791-tqchfdmy	158	9	to	to	IN
cord-002791-tqchfdmy	158	10	the	the	DT
cord-002791-tqchfdmy	158	11	manufacturer	manufacturer	NN
cord-002791-tqchfdmy	158	12	's	's	POS
cord-002791-tqchfdmy	158	13	recommendations	recommendation	NNS
cord-002791-tqchfdmy	158	14	.	.	.
cord-002791-tqchfdmy	159	1	Transformed	transform	VBN
cord-002791-tqchfdmy	159	2	cells	cell	NNS
cord-002791-tqchfdmy	159	3	were	be	VBD
cord-002791-tqchfdmy	159	4	grown	grow	VBN
cord-002791-tqchfdmy	159	5	on	on	IN
cord-002791-tqchfdmy	159	6	LB	LB	NNP
cord-002791-tqchfdmy	159	7	(	(	-LRB-
cord-002791-tqchfdmy	159	8	Sigma	Sigma	NNP
cord-002791-tqchfdmy	159	9	-	-	HYPH
cord-002791-tqchfdmy	159	10	Aldrich	Aldrich	NNP
cord-002791-tqchfdmy	159	11	)	)	-RRB-
cord-002791-tqchfdmy	159	12	agar	agar	NNP
cord-002791-tqchfdmy	159	13	plates	plate	NNS
cord-002791-tqchfdmy	159	14	containing	contain	VBG
cord-002791-tqchfdmy	159	15	chloramphenicol	chloramphenicol	NNP
cord-002791-tqchfdmy	159	16	(	(	-LRB-
cord-002791-tqchfdmy	159	17	34	34	CD
cord-002791-tqchfdmy	159	18	µg	µg	NN
cord-002791-tqchfdmy	159	19	/	/	SYM
cord-002791-tqchfdmy	159	20	ml	ml	NN
cord-002791-tqchfdmy	159	21	)	)	-RRB-
cord-002791-tqchfdmy	159	22	overnight	overnight	RB
cord-002791-tqchfdmy	159	23	.	.	.
cord-002791-tqchfdmy	160	1	Plasmid	Plasmid	NNP
cord-002791-tqchfdmy	160	2	DNA	DNA	NNP
cord-002791-tqchfdmy	160	3	was	be	VBD
cord-002791-tqchfdmy	160	4	isolated	isolate	VBN
cord-002791-tqchfdmy	160	5	with	with	IN
cord-002791-tqchfdmy	160	6	the	the	DT
cord-002791-tqchfdmy	160	7	NucleoSpin	NucleoSpin	NNP
cord-002791-tqchfdmy	160	8	Plasmid	Plasmid	NNP
cord-002791-tqchfdmy	160	9	kit	kit	NN
cord-002791-tqchfdmy	160	10	(	(	-LRB-
cord-002791-tqchfdmy	160	11	Macherey	Macherey	NNP
cord-002791-tqchfdmy	160	12	-	-	HYPH
cord-002791-tqchfdmy	160	13	Nagel	Nagel	NNP
cord-002791-tqchfdmy	160	14	)	)	-RRB-
cord-002791-tqchfdmy	160	15	according	accord	VBG
cord-002791-tqchfdmy	160	16	to	to	IN
cord-002791-tqchfdmy	160	17	the	the	DT
cord-002791-tqchfdmy	160	18	manufacturer	manufacturer	NN
cord-002791-tqchfdmy	160	19	's	's	POS
cord-002791-tqchfdmy	160	20	instructions	instruction	NNS
cord-002791-tqchfdmy	160	21	.	.	.
cord-002791-tqchfdmy	161	1	The	the	DT
cord-002791-tqchfdmy	161	2	presence	presence	NN
cord-002791-tqchfdmy	161	3	of	of	IN
cord-002791-tqchfdmy	161	4	specific	specific	JJ
cord-002791-tqchfdmy	161	5	inserts	insert	NNS
cord-002791-tqchfdmy	161	6	was	be	VBD
cord-002791-tqchfdmy	161	7	confirmed	confirm	VBN
cord-002791-tqchfdmy	161	8	by	by	IN
cord-002791-tqchfdmy	161	9	vector	vector	NN
cord-002791-tqchfdmy	161	10	-	-	HYPH
cord-002791-tqchfdmy	161	11	specific	specific	JJ
cord-002791-tqchfdmy	161	12	(	(	-LRB-
cord-002791-tqchfdmy	161	13	DuetUP1	DuetUP1	NNP
cord-002791-tqchfdmy	161	14	and	and	CC
cord-002791-tqchfdmy	161	15	DuetDOWN1	DuetDOWN1	NNP
cord-002791-tqchfdmy	161	16	)	)	-RRB-
cord-002791-tqchfdmy	161	17	and	and	CC
cord-002791-tqchfdmy	161	18	insert	insert	VB
cord-002791-tqchfdmy	161	19	-	-	HYPH
cord-002791-tqchfdmy	161	20	specific	specific	JJ
cord-002791-tqchfdmy	161	21	(	(	-LRB-
cord-002791-tqchfdmy	161	22	MS2	MS2	NNP
cord-002791-tqchfdmy	161	23	NcoI	NcoI	NNP
cord-002791-tqchfdmy	161	24	and	and	CC
cord-002791-tqchfdmy	161	25	MS2	MS2	NNP
cord-002791-tqchfdmy	161	26	NotI	NotI	NNP
cord-002791-tqchfdmy	161	27	)	)	-RRB-
cord-002791-tqchfdmy	161	28	primers	primer	NNS
cord-002791-tqchfdmy	161	29	(	(	-LRB-
cord-002791-tqchfdmy	161	30	Table	Table	NNP
cord-002791-tqchfdmy	161	31	3	3	CD
cord-002791-tqchfdmy	161	32	)	)	-RRB-
cord-002791-tqchfdmy	161	33	in	in	IN
cord-002791-tqchfdmy	161	34	PCRs	pcr	NNS
cord-002791-tqchfdmy	161	35	as	as	RB
cord-002791-tqchfdmy	161	36	well	well	RB
cord-002791-tqchfdmy	161	37	as	as	IN
cord-002791-tqchfdmy	161	38	by	by	IN
cord-002791-tqchfdmy	161	39	sequencing	sequence	VBG
cord-002791-tqchfdmy	161	40	(	(	-LRB-
cord-002791-tqchfdmy	161	41	Eurofins	Eurofins	NNP
cord-002791-tqchfdmy	161	42	MWG	MWG	NNP
cord-002791-tqchfdmy	161	43	Operon	Operon	NNP
cord-002791-tqchfdmy	161	44	)	)	-RRB-
cord-002791-tqchfdmy	161	45	.	.	.
cord-002791-tqchfdmy	162	1	The	the	DT
cord-002791-tqchfdmy	162	2	constructed	construct	VBN
cord-002791-tqchfdmy	162	3	pACYCDuet-1-TM	pacycduet-1-tm	CD
cord-002791-tqchfdmy	162	4	-	-	HYPH
cord-002791-tqchfdmy	162	5	CoatDimer-	coatdimer-	NN
cord-002791-tqchfdmy	163	1	His	-PRON-	PRP$
cord-002791-tqchfdmy	163	2	plasmid	plasmid	NN
cord-002791-tqchfdmy	163	3	was	be	VBD
cord-002791-tqchfdmy	163	4	transformed	transform	VBN
cord-002791-tqchfdmy	163	5	into	into	IN
cord-002791-tqchfdmy	163	6	E.	E.	NNP
cord-002791-tqchfdmy	163	7	coli	coli	NNS
cord-002791-tqchfdmy	164	1	BL21	BL21	NNP
cord-002791-tqchfdmy	164	2	(	(	-LRB-
cord-002791-tqchfdmy	164	3	DE3	DE3	NNP
cord-002791-tqchfdmy	164	4	)	)	-RRB-
cord-002791-tqchfdmy	164	5	cells	cell	NNS
cord-002791-tqchfdmy	164	6	(	(	-LRB-
cord-002791-tqchfdmy	164	7	NEB	NEB	NNP
cord-002791-tqchfdmy	164	8	)	)	-RRB-
cord-002791-tqchfdmy	165	1	according	accord	VBG
cord-002791-tqchfdmy	165	2	to	to	IN
cord-002791-tqchfdmy	165	3	the	the	DT
cord-002791-tqchfdmy	165	4	manufacturer	manufacturer	NN
cord-002791-tqchfdmy	165	5	's	's	POS
cord-002791-tqchfdmy	165	6	instructions	instruction	NNS
cord-002791-tqchfdmy	165	7	and	and	CC
cord-002791-tqchfdmy	165	8	the	the	DT
cord-002791-tqchfdmy	165	9	bacterial	bacterial	JJ
cord-002791-tqchfdmy	165	10	cells	cell	NNS
cord-002791-tqchfdmy	165	11	were	be	VBD
cord-002791-tqchfdmy	165	12	grown	grow	VBN
cord-002791-tqchfdmy	165	13	in	in	IN
cord-002791-tqchfdmy	165	14	LB	LB	NNP
cord-002791-tqchfdmy	165	15	broth	broth	NN
cord-002791-tqchfdmy	165	16	(	(	-LRB-
cord-002791-tqchfdmy	165	17	Sigma	Sigma	NNP
cord-002791-tqchfdmy	165	18	-	-	HYPH
cord-002791-tqchfdmy	165	19	Aldrich	Aldrich	NNP
cord-002791-tqchfdmy	165	20	)	)	-RRB-
cord-002791-tqchfdmy	165	21	containing	contain	VBG
cord-002791-tqchfdmy	165	22	chloramphenicol	chloramphenicol	NNP
cord-002791-tqchfdmy	165	23	(	(	-LRB-
cord-002791-tqchfdmy	165	24	34	34	CD
cord-002791-tqchfdmy	165	25	µg	µg	NN
cord-002791-tqchfdmy	165	26	/	/	SYM
cord-002791-tqchfdmy	165	27	ml	ml	NN
cord-002791-tqchfdmy	165	28	)	)	-RRB-
cord-002791-tqchfdmy	165	29	at	at	IN
cord-002791-tqchfdmy	165	30	37	37	CD
cord-002791-tqchfdmy	165	31	°	°	NN
cord-002791-tqchfdmy	165	32	C	c	NN
cord-002791-tqchfdmy	165	33	until	until	IN
cord-002791-tqchfdmy	165	34	OD	OD	NNP
cord-002791-tqchfdmy	165	35	600	600	CD
cord-002791-tqchfdmy	165	36	=	=	SYM
cord-002791-tqchfdmy	165	37	1.7	1.7	CD
cord-002791-tqchfdmy	165	38	.	.	.
cord-002791-tqchfdmy	166	1	Two	two	CD
cord-002791-tqchfdmy	166	2	ml	ml	NNS
cord-002791-tqchfdmy	166	3	of	of	IN
cord-002791-tqchfdmy	166	4	the	the	DT
cord-002791-tqchfdmy	166	5	bacterial	bacterial	JJ
cord-002791-tqchfdmy	166	6	culture	culture	NN
cord-002791-tqchfdmy	166	7	were	be	VBD
cord-002791-tqchfdmy	166	8	transferred	transfer	VBN
cord-002791-tqchfdmy	166	9	to	to	IN
cord-002791-tqchfdmy	166	10	200	200	CD
cord-002791-tqchfdmy	166	11	ml	ml	NNS
cord-002791-tqchfdmy	166	12	of	of	IN
cord-002791-tqchfdmy	166	13	Terrific	terrific	JJ
cord-002791-tqchfdmy	166	14	broth	broth	NN
cord-002791-tqchfdmy	166	15	(	(	-LRB-
cord-002791-tqchfdmy	166	16	TB	TB	NNP
cord-002791-tqchfdmy	166	17	)	)	-RRB-
cord-002791-tqchfdmy	166	18	medium	medium	NN
cord-002791-tqchfdmy	167	1	(	(	-LRB-
cord-002791-tqchfdmy	167	2	24	24	CD
cord-002791-tqchfdmy	167	3	g	g	NN
cord-002791-tqchfdmy	167	4	/	/	SYM
cord-002791-tqchfdmy	167	5	l	l	NN
cord-002791-tqchfdmy	167	6	yeast	yeast	NN
cord-002791-tqchfdmy	167	7	extract	extract	NN
cord-002791-tqchfdmy	167	8	,	,	,
cord-002791-tqchfdmy	167	9	20	20	CD
cord-002791-tqchfdmy	167	10	g	g	NN
cord-002791-tqchfdmy	167	11	/	/	SYM
cord-002791-tqchfdmy	167	12	l	l	NN
cord-002791-tqchfdmy	167	13	tryptone	tryptone	NN
cord-002791-tqchfdmy	167	14	,	,	,
cord-002791-tqchfdmy	167	15	4	4	CD
cord-002791-tqchfdmy	167	16	ml	ml	NN
cord-002791-tqchfdmy	167	17	/	/	SYM
cord-002791-tqchfdmy	167	18	l	l	NN
cord-002791-tqchfdmy	167	19	glycerol	glycerol	NN
cord-002791-tqchfdmy	167	20	,	,	,
cord-002791-tqchfdmy	167	21	0.017	0.017	CD
cord-002791-tqchfdmy	167	22	M	M	NNP
cord-002791-tqchfdmy	167	23	KH	KH	NNP
cord-002791-tqchfdmy	167	24	2	2	CD
cord-002791-tqchfdmy	167	25	PO	PO	NNP
cord-002791-tqchfdmy	167	26	4	4	CD
cord-002791-tqchfdmy	167	27	,	,	,
cord-002791-tqchfdmy	167	28	0.072	0.072	CD
cord-002791-tqchfdmy	167	29	M	m	NN
cord-002791-tqchfdmy	167	30	K	K	NNP
cord-002791-tqchfdmy	167	31	2	2	CD
cord-002791-tqchfdmy	167	32	HPO	HPO	NNP
cord-002791-tqchfdmy	167	33	4	4	CD
cord-002791-tqchfdmy	167	34	,	,	,
cord-002791-tqchfdmy	167	35	all	all	DT
cord-002791-tqchfdmy	167	36	Sigma	Sigma	NNP
cord-002791-tqchfdmy	167	37	-	-	HYPH
cord-002791-tqchfdmy	167	38	Aldrich	Aldrich	NNP
cord-002791-tqchfdmy	167	39	)	)	-RRB-
cord-002791-tqchfdmy	167	40	containing	contain	VBG
cord-002791-tqchfdmy	167	41	chloramphenicol	chloramphenicol	NNP
cord-002791-tqchfdmy	167	42	(	(	-LRB-
cord-002791-tqchfdmy	167	43	34	34	CD
cord-002791-tqchfdmy	167	44	µg	µg	NN
cord-002791-tqchfdmy	167	45	/	/	SYM
cord-002791-tqchfdmy	167	46	ml	ml	NN
cord-002791-tqchfdmy	167	47	)	)	-RRB-
cord-002791-tqchfdmy	167	48	and	and	CC
cord-002791-tqchfdmy	167	49	cultivated	cultivate	VBN
cord-002791-tqchfdmy	167	50	at	at	IN
cord-002791-tqchfdmy	167	51	37	37	CD
cord-002791-tqchfdmy	167	52	°	°	NN
cord-002791-tqchfdmy	167	53	C	c	NN
cord-002791-tqchfdmy	167	54	until	until	IN
cord-002791-tqchfdmy	167	55	OD	OD	NNP
cord-002791-tqchfdmy	167	56	600	600	CD
cord-002791-tqchfdmy	167	57	=	=	SYM
cord-002791-tqchfdmy	167	58	0.8	0.8	CD
cord-002791-tqchfdmy	167	59	and	and	CC
cord-002791-tqchfdmy	167	60	centrifuged	centrifuge	VBN
cord-002791-tqchfdmy	167	61	at	at	IN
cord-002791-tqchfdmy	167	62	6000	6000	CD
cord-002791-tqchfdmy	167	63	×	×	NN
cord-002791-tqchfdmy	167	64	g	g	NN
cord-002791-tqchfdmy	167	65	for	for	IN
cord-002791-tqchfdmy	167	66	10	10	CD
cord-002791-tqchfdmy	167	67	minutes	minute	NNS
cord-002791-tqchfdmy	167	68	at	at	IN
cord-002791-tqchfdmy	167	69	25	25	CD
cord-002791-tqchfdmy	167	70	°	°	NN
cord-002791-tqchfdmy	167	71	C	c	NN
cord-002791-tqchfdmy	167	72	.	.	.
cord-002791-tqchfdmy	168	1	Subsequently	subsequently	RB
cord-002791-tqchfdmy	168	2	,	,	,
cord-002791-tqchfdmy	168	3	the	the	DT
cord-002791-tqchfdmy	168	4	pellet	pellet	NN
cord-002791-tqchfdmy	168	5	was	be	VBD
cord-002791-tqchfdmy	168	6	resuspended	resuspend	VBN
cord-002791-tqchfdmy	168	7	in	in	IN
cord-002791-tqchfdmy	168	8	200	200	CD
cord-002791-tqchfdmy	168	9	ml	ml	NNS
cord-002791-tqchfdmy	168	10	of	of	IN
cord-002791-tqchfdmy	168	11	fresh	fresh	JJ
cord-002791-tqchfdmy	168	12	TB	TB	NNP
cord-002791-tqchfdmy	168	13	medium	medium	NN
cord-002791-tqchfdmy	168	14	containing	contain	VBG
cord-002791-tqchfdmy	168	15	chloramphenicol	chloramphenicol	NNP
cord-002791-tqchfdmy	168	16	(	(	-LRB-
cord-002791-tqchfdmy	168	17	34	34	CD
cord-002791-tqchfdmy	168	18	µg	µg	NN
cord-002791-tqchfdmy	168	19	/	/	SYM
cord-002791-tqchfdmy	168	20	ml	ml	NN
cord-002791-tqchfdmy	168	21	)	)	-RRB-
cord-002791-tqchfdmy	168	22	.	.	.
cord-002791-tqchfdmy	169	1	Protein	protein	NN
cord-002791-tqchfdmy	169	2	expression	expression	NN
cord-002791-tqchfdmy	169	3	was	be	VBD
cord-002791-tqchfdmy	169	4	induced	induce	VBN
cord-002791-tqchfdmy	169	5	by	by	IN
cord-002791-tqchfdmy	169	6	addition	addition	NN
cord-002791-tqchfdmy	169	7	of	of	IN
cord-002791-tqchfdmy	169	8	2	2	CD
cord-002791-tqchfdmy	169	9	mM	mm	CD
cord-002791-tqchfdmy	169	10	isopropyl	isopropyl	NN
cord-002791-tqchfdmy	169	11	-	-	HYPH
cord-002791-tqchfdmy	169	12	L	l	NN
cord-002791-tqchfdmy	169	13	-	-	HYPH
cord-002791-tqchfdmy	169	14	thio	thio	NNP
cord-002791-tqchfdmy	169	15	-	-	HYPH
cord-002791-tqchfdmy	169	16	D	D	NNP
cord-002791-tqchfdmy	169	17	-	-	HYPH
cord-002791-tqchfdmy	169	18	galactopyranoside	galactopyranoside	NNP
cord-002791-tqchfdmy	169	19	(	(	-LRB-
cord-002791-tqchfdmy	169	20	IPTG	IPTG	NNP
cord-002791-tqchfdmy	169	21	)	)	-RRB-
cord-002791-tqchfdmy	170	1	(	(	-LRB-
cord-002791-tqchfdmy	170	2	Sigma	Sigma	NNP
cord-002791-tqchfdmy	170	3	-	-	HYPH
cord-002791-tqchfdmy	170	4	Aldrich	Aldrich	NNP
cord-002791-tqchfdmy	170	5	)	)	-RRB-
cord-002791-tqchfdmy	170	6	to	to	IN
cord-002791-tqchfdmy	170	7	the	the	DT
cord-002791-tqchfdmy	170	8	culture	culture	NN
cord-002791-tqchfdmy	170	9	,	,	,
cord-002791-tqchfdmy	170	10	after	after	IN
cord-002791-tqchfdmy	170	11	30	30	CD
cord-002791-tqchfdmy	170	12	minutes	minute	NNS
cord-002791-tqchfdmy	170	13	of	of	IN
cord-002791-tqchfdmy	170	14	culture	culture	NN
cord-002791-tqchfdmy	170	15	growth	growth	NN
cord-002791-tqchfdmy	170	16	at	at	IN
cord-002791-tqchfdmy	170	17	37	37	CD
cord-002791-tqchfdmy	170	18	°	°	NN
cord-002791-tqchfdmy	170	19	C	c	NN
cord-002791-tqchfdmy	170	20	,	,	,
cord-002791-tqchfdmy	170	21	rifampicin	rifampicin	NNS
cord-002791-tqchfdmy	170	22	(	(	-LRB-
cord-002791-tqchfdmy	170	23	Sigma	Sigma	NNP
cord-002791-tqchfdmy	170	24	-	-	HYPH
cord-002791-tqchfdmy	170	25	Aldrich	Aldrich	NNP
cord-002791-tqchfdmy	170	26	)	)	-RRB-
cord-002791-tqchfdmy	170	27	was	be	VBD
cord-002791-tqchfdmy	170	28	added	add	VBN
cord-002791-tqchfdmy	170	29	to	to	IN
cord-002791-tqchfdmy	170	30	the	the	DT
cord-002791-tqchfdmy	170	31	IPTG	IPTG	NNP
cord-002791-tqchfdmy	170	32	induced	induce	VBN
cord-002791-tqchfdmy	170	33	culture	culture	NN
cord-002791-tqchfdmy	170	34	to	to	IN
cord-002791-tqchfdmy	170	35	a	a	DT
cord-002791-tqchfdmy	170	36	final	final	JJ
cord-002791-tqchfdmy	170	37	concentration	concentration	NN
cord-002791-tqchfdmy	170	38	of	of	IN
cord-002791-tqchfdmy	170	39	200	200	CD
cord-002791-tqchfdmy	170	40	µg	µg	NN
cord-002791-tqchfdmy	170	41	/	/	SYM
cord-002791-tqchfdmy	170	42	ml	ml	NN
cord-002791-tqchfdmy	170	43	and	and	CC
cord-002791-tqchfdmy	170	44	the	the	DT
cord-002791-tqchfdmy	170	45	culture	culture	NN
cord-002791-tqchfdmy	170	46	was	be	VBD
cord-002791-tqchfdmy	170	47	cultivated	cultivate	VBN
cord-002791-tqchfdmy	170	48	at	at	IN
cord-002791-tqchfdmy	170	49	37	37	CD
cord-002791-tqchfdmy	170	50	°	°	NN
cord-002791-tqchfdmy	170	51	C	c	NN
cord-002791-tqchfdmy	170	52	for	for	IN
cord-002791-tqchfdmy	170	53	16	16	CD
cord-002791-tqchfdmy	170	54	hours	hour	NNS
cord-002791-tqchfdmy	170	55	.	.	.
cord-002791-tqchfdmy	171	1	The	the	DT
cord-002791-tqchfdmy	171	2	cell	cell	NN
cord-002791-tqchfdmy	171	3	suspension	suspension	NN
cord-002791-tqchfdmy	171	4	was	be	VBD
cord-002791-tqchfdmy	171	5	centrifuged	centrifuge	VBN
cord-002791-tqchfdmy	171	6	at	at	IN
cord-002791-tqchfdmy	171	7	6000	6000	CD
cord-002791-tqchfdmy	171	8	×	×	NN
cord-002791-tqchfdmy	171	9	g	g	NN
cord-002791-tqchfdmy	171	10	for	for	IN
cord-002791-tqchfdmy	171	11	10	10	CD
cord-002791-tqchfdmy	171	12	minutes	minute	NNS
cord-002791-tqchfdmy	171	13	at	at	IN
cord-002791-tqchfdmy	171	14	4	4	CD
cord-002791-tqchfdmy	171	15	°	°	NN
cord-002791-tqchfdmy	171	16	C	c	NN
cord-002791-tqchfdmy	171	17	and	and	CC
cord-002791-tqchfdmy	171	18	cells	cell	NNS
cord-002791-tqchfdmy	171	19	were	be	VBD
cord-002791-tqchfdmy	171	20	washed	wash	VBN
cord-002791-tqchfdmy	171	21	and	and	CC
cord-002791-tqchfdmy	171	22	centrifuged	centrifuge	VBN
cord-002791-tqchfdmy	171	23	(	(	-LRB-
cord-002791-tqchfdmy	171	24	6000	6000	CD
cord-002791-tqchfdmy	171	25	×	×	NN
cord-002791-tqchfdmy	171	26	g	g	NN
cord-002791-tqchfdmy	171	27	for	for	IN
cord-002791-tqchfdmy	171	28	10	10	CD
cord-002791-tqchfdmy	171	29	minutes	minute	NNS
cord-002791-tqchfdmy	171	30	at	at	IN
cord-002791-tqchfdmy	171	31	4	4	CD
cord-002791-tqchfdmy	171	32	°	°	NN
cord-002791-tqchfdmy	171	33	C	c	NN
cord-002791-tqchfdmy	171	34	)	)	-RRB-
cord-002791-tqchfdmy	171	35	twice	twice	RB
cord-002791-tqchfdmy	171	36	in	in	IN
cord-002791-tqchfdmy	171	37	200	200	CD
cord-002791-tqchfdmy	171	38	ml	ml	NNS
cord-002791-tqchfdmy	171	39	of	of	IN
cord-002791-tqchfdmy	171	40	PBS	PBS	NNP
cord-002791-tqchfdmy	171	41	buffer	buffer	NN
cord-002791-tqchfdmy	172	1	(	(	-LRB-
cord-002791-tqchfdmy	172	2	pH	ph	NN
cord-002791-tqchfdmy	172	3	=	=	NNP
cord-002791-tqchfdmy	172	4	7.2	7.2	CD
cord-002791-tqchfdmy	172	5	)	)	-RRB-
cord-002791-tqchfdmy	172	6	.	.	.
cord-002791-tqchfdmy	173	1	The	the	DT
cord-002791-tqchfdmy	173	2	pellet	pellet	NN
cord-002791-tqchfdmy	173	3	was	be	VBD
cord-002791-tqchfdmy	173	4	resuspended	resuspend	VBN
cord-002791-tqchfdmy	173	5	in	in	IN
cord-002791-tqchfdmy	173	6	4	4	CD
cord-002791-tqchfdmy	173	7	ml	ml	NNS
cord-002791-tqchfdmy	173	8	of	of	IN
cord-002791-tqchfdmy	173	9	sonication	sonication	NN
cord-002791-tqchfdmy	173	10	buffer	buffer	NN
cord-002791-tqchfdmy	173	11	(	(	-LRB-
cord-002791-tqchfdmy	173	12	50	50	CD
cord-002791-tqchfdmy	173	13	mM	mm	CD
cord-002791-tqchfdmy	173	14	Tris	Tris	NNP
cord-002791-tqchfdmy	173	15	,	,	,
cord-002791-tqchfdmy	173	16	5	5	CD
cord-002791-tqchfdmy	173	17	mM	mm	CD
cord-002791-tqchfdmy	173	18	MgCl	MgCl	NNP
cord-002791-tqchfdmy	173	19	2	2	CD
cord-002791-tqchfdmy	173	20	×	×	NN
cord-002791-tqchfdmy	173	21	6H	6h	NN
cord-002791-tqchfdmy	173	22	2	2	CD
cord-002791-tqchfdmy	173	23	O	o	NN
cord-002791-tqchfdmy	173	24	,	,	,
cord-002791-tqchfdmy	173	25	5	5	CD
cord-002791-tqchfdmy	173	26	mM	mm	CD
cord-002791-tqchfdmy	173	27	CaCl	cacl	NN
cord-002791-tqchfdmy	173	28	2	2	CD
cord-002791-tqchfdmy	173	29	,	,	,
cord-002791-tqchfdmy	173	30	0.1	0.1	CD
cord-002791-tqchfdmy	173	31	M	m	NN
cord-002791-tqchfdmy	173	32	NaCl	nacl	NN
cord-002791-tqchfdmy	173	33	,	,	,
cord-002791-tqchfdmy	173	34	pH	ph	NN
cord-002791-tqchfdmy	173	35	=	=	NNP
cord-002791-tqchfdmy	173	36	8.0	8.0	CD
cord-002791-tqchfdmy	173	37	,	,	,
cord-002791-tqchfdmy	173	38	all	all	DT
cord-002791-tqchfdmy	173	39	Sigma	Sigma	NNP
cord-002791-tqchfdmy	173	40	-	-	HYPH
cord-002791-tqchfdmy	173	41	Aldrich	Aldrich	NNP
cord-002791-tqchfdmy	173	42	)	)	-RRB-
cord-002791-tqchfdmy	173	43	and	and	CC
cord-002791-tqchfdmy	173	44	200	200	CD
cord-002791-tqchfdmy	173	45	U	u	NN
cord-002791-tqchfdmy	173	46	of	of	IN
cord-002791-tqchfdmy	173	47	Turbo	Turbo	NNP
cord-002791-tqchfdmy	173	48	DNase	DNase	NNP
cord-002791-tqchfdmy	173	49	(	(	-LRB-
cord-002791-tqchfdmy	173	50	Ambion	Ambion	NNP
cord-002791-tqchfdmy	173	51	,	,	,
cord-002791-tqchfdmy	173	52	ThermoFisher	ThermoFisher	NNP
cord-002791-tqchfdmy	173	53	Scientific	Scientific	NNP
cord-002791-tqchfdmy	173	54	,	,	,
cord-002791-tqchfdmy	173	55	USA	USA	NNP
cord-002791-tqchfdmy	173	56	)	)	-RRB-
cord-002791-tqchfdmy	173	57	,	,	,
cord-002791-tqchfdmy	173	58	700	700	CD
cord-002791-tqchfdmy	173	59	U	u	NN
cord-002791-tqchfdmy	173	60	of	of	IN
cord-002791-tqchfdmy	173	61	RNase	RNase	NNP
cord-002791-tqchfdmy	173	62	A	A	NNP
cord-002791-tqchfdmy	173	63	(	(	-LRB-
cord-002791-tqchfdmy	173	64	Qiagen	Qiagen	NNP
cord-002791-tqchfdmy	173	65	)	)	-RRB-
cord-002791-tqchfdmy	173	66	,	,	,
cord-002791-tqchfdmy	173	67	and	and	CC
cord-002791-tqchfdmy	174	1	2500	2500	CD
cord-002791-tqchfdmy	174	2	U	U	NNP
cord-002791-tqchfdmy	174	3	of	of	IN
cord-002791-tqchfdmy	174	4	benzonase	benzonase	NNP
cord-002791-tqchfdmy	174	5	nuclease	nuclease	NNP
cord-002791-tqchfdmy	174	6	(	(	-LRB-
cord-002791-tqchfdmy	174	7	Sigma	Sigma	NNP
cord-002791-tqchfdmy	174	8	-	-	HYPH
cord-002791-tqchfdmy	174	9	Aldrich	Aldrich	NNP
cord-002791-tqchfdmy	174	10	)	)	-RRB-
cord-002791-tqchfdmy	174	11	were	be	VBD
cord-002791-tqchfdmy	174	12	added	add	VBN
cord-002791-tqchfdmy	174	13	to	to	TO
cord-002791-tqchfdmy	174	14	eliminate	eliminate	VB
cord-002791-tqchfdmy	174	15	E.	E.	NNP
cord-002791-tqchfdmy	174	16	coli	coli	NNS
cord-002791-tqchfdmy	174	17	genomic	genomic	JJ
cord-002791-tqchfdmy	174	18	DNA	DNA	NNP
cord-002791-tqchfdmy	174	19	and	and	CC
cord-002791-tqchfdmy	174	20	RNA	RNA	NNP
cord-002791-tqchfdmy	174	21	.	.	.
cord-002791-tqchfdmy	175	1	The	the	DT
cord-002791-tqchfdmy	175	2	cells	cell	NNS
cord-002791-tqchfdmy	175	3	were	be	VBD
cord-002791-tqchfdmy	175	4	lysed	lyse	VBN
cord-002791-tqchfdmy	175	5	by	by	IN
cord-002791-tqchfdmy	175	6	ultrasonic	ultrasonic	JJ
cord-002791-tqchfdmy	175	7	disruption	disruption	NN
cord-002791-tqchfdmy	175	8	(	(	-LRB-
cord-002791-tqchfdmy	175	9	Bandelin	Bandelin	NNP
cord-002791-tqchfdmy	175	10	VW3100	VW3100	NNP
cord-002791-tqchfdmy	175	11	sonicator	sonicator	NN
cord-002791-tqchfdmy	175	12	,	,	,
cord-002791-tqchfdmy	175	13	probe	probe	VB
cord-002791-tqchfdmy	175	14	MS73	MS73	NNP
cord-002791-tqchfdmy	175	15	,	,	,
cord-002791-tqchfdmy	175	16	Bandelin	Bandelin	NNP
cord-002791-tqchfdmy	175	17	,	,	,
cord-002791-tqchfdmy	175	18	Germany	Germany	NNP
cord-002791-tqchfdmy	175	19	)	)	-RRB-
cord-002791-tqchfdmy	175	20	at	at	IN
cord-002791-tqchfdmy	175	21	amplitude	amplitude	NN
cord-002791-tqchfdmy	175	22	50	50	CD
cord-002791-tqchfdmy	175	23	%	%	NN
cord-002791-tqchfdmy	175	24	,	,	,
cord-002791-tqchfdmy	175	25	using	use	VBG
cord-002791-tqchfdmy	175	26	four	four	CD
cord-002791-tqchfdmy	175	27	pulses	pulse	NNS
cord-002791-tqchfdmy	175	28	for	for	IN
cord-002791-tqchfdmy	175	29	a	a	DT
cord-002791-tqchfdmy	175	30	total	total	JJ
cord-002791-tqchfdmy	175	31	length	length	NN
cord-002791-tqchfdmy	175	32	of	of	IN
cord-002791-tqchfdmy	175	33	2	2	CD
cord-002791-tqchfdmy	175	34	minutes	minute	NNS
cord-002791-tqchfdmy	175	35	at	at	IN
cord-002791-tqchfdmy	175	36	4	4	CD
cord-002791-tqchfdmy	175	37	°	°	NN
cord-002791-tqchfdmy	175	38	C	c	NN
cord-002791-tqchfdmy	175	39	.	.	.
cord-002791-tqchfdmy	176	1	Sonicated	sonicated	JJ
cord-002791-tqchfdmy	176	2	bacterial	bacterial	JJ
cord-002791-tqchfdmy	176	3	suspension	suspension	NN
cord-002791-tqchfdmy	176	4	was	be	VBD
cord-002791-tqchfdmy	176	5	incubated	incubate	VBN
cord-002791-tqchfdmy	176	6	at	at	IN
cord-002791-tqchfdmy	176	7	37	37	CD
cord-002791-tqchfdmy	176	8	°	°	NN
cord-002791-tqchfdmy	176	9	C	c	NN
cord-002791-tqchfdmy	176	10	for	for	IN
cord-002791-tqchfdmy	176	11	3	3	CD
cord-002791-tqchfdmy	176	12	hours	hour	NNS
cord-002791-tqchfdmy	176	13	.	.	.
cord-002791-tqchfdmy	177	1	To	to	TO
cord-002791-tqchfdmy	177	2	eliminate	eliminate	VB
cord-002791-tqchfdmy	177	3	cell	cell	NN
cord-002791-tqchfdmy	177	4	debris	debris	NN
cord-002791-tqchfdmy	177	5	,	,	,
cord-002791-tqchfdmy	177	6	the	the	DT
cord-002791-tqchfdmy	177	7	lysed	lyse	VBN
cord-002791-tqchfdmy	177	8	bacterial	bacterial	JJ
cord-002791-tqchfdmy	177	9	suspension	suspension	NN
cord-002791-tqchfdmy	177	10	was	be	VBD
cord-002791-tqchfdmy	177	11	briefly	briefly	RB
cord-002791-tqchfdmy	177	12	centrifuged	centrifuge	VBN
cord-002791-tqchfdmy	177	13	at	at	IN
cord-002791-tqchfdmy	177	14	6000×	6000×	CD
cord-002791-tqchfdmy	177	15	g	g	NN
cord-002791-tqchfdmy	177	16	for	for	IN
cord-002791-tqchfdmy	177	17	15	15	CD
cord-002791-tqchfdmy	177	18	minutes	minute	NNS
cord-002791-tqchfdmy	177	19	at	at	IN
cord-002791-tqchfdmy	177	20	room	room	NN
cord-002791-tqchfdmy	177	21	temperature	temperature	NN
cord-002791-tqchfdmy	177	22	and	and	CC
cord-002791-tqchfdmy	177	23	the	the	DT
cord-002791-tqchfdmy	177	24	supernatant	supernatant	NN
cord-002791-tqchfdmy	177	25	containing	contain	VBG
cord-002791-tqchfdmy	177	26	His	-PRON-	PRP$
cord-002791-tqchfdmy	177	27	-	-	HYPH
cord-002791-tqchfdmy	177	28	tagged	tag	VBN
cord-002791-tqchfdmy	177	29	MS2	MS2	NNP
cord-002791-tqchfdmy	177	30	PLP	PLP	NNP
cord-002791-tqchfdmy	177	31	was	be	VBD
cord-002791-tqchfdmy	177	32	filtered	filter	VBN
cord-002791-tqchfdmy	177	33	through	through	IN
cord-002791-tqchfdmy	177	34	a	a	DT
cord-002791-tqchfdmy	177	35	0.22-µm	0.22-µm	CD
cord-002791-tqchfdmy	177	36	syringe	syringe	NN
cord-002791-tqchfdmy	177	37	PES	PES	NNP
cord-002791-tqchfdmy	177	38	filter	filter	NN
cord-002791-tqchfdmy	177	39	(	(	-LRB-
cord-002791-tqchfdmy	177	40	TPP	TPP	NNP
cord-002791-tqchfdmy	177	41	,	,	,
cord-002791-tqchfdmy	177	42	Switzerland	Switzerland	NNP
cord-002791-tqchfdmy	177	43	)	)	-RRB-
cord-002791-tqchfdmy	177	44	.	.	.
cord-002791-tqchfdmy	178	1	Tris	Tris	NNP
cord-002791-tqchfdmy	178	2	,	,	,
cord-002791-tqchfdmy	178	3	100	100	CD
cord-002791-tqchfdmy	178	4	NaCl	nacl	NN
cord-002791-tqchfdmy	178	5	,	,	,
cord-002791-tqchfdmy	178	6	1	1	CD
cord-002791-tqchfdmy	178	7	mM	mm	CD
cord-002791-tqchfdmy	178	8	EDTA	EDTA	NNP
cord-002791-tqchfdmy	178	9	,	,	,
cord-002791-tqchfdmy	178	10	pH	ph	NN
cord-002791-tqchfdmy	178	11	=	=	SYM
cord-002791-tqchfdmy	178	12	7.5	7.5	CD
cord-002791-tqchfdmy	178	13	,	,	,
cord-002791-tqchfdmy	178	14	all	all	DT
cord-002791-tqchfdmy	178	15	Sigma	Sigma	NNP
cord-002791-tqchfdmy	178	16	-	-	HYPH
cord-002791-tqchfdmy	178	17	Aldrich	Aldrich	NNP
cord-002791-tqchfdmy	178	18	)	)	-RRB-
cord-002791-tqchfdmy	178	19	.	.	.
cord-002791-tqchfdmy	179	1	His	-PRON-	PRP$
cord-002791-tqchfdmy	179	2	-	-	HYPH
cord-002791-tqchfdmy	179	3	tagged	tag	VBN
cord-002791-tqchfdmy	179	4	MS2	MS2	NNP
cord-002791-tqchfdmy	179	5	PLP	PLP	NNP
cord-002791-tqchfdmy	179	6	were	be	VBD
cord-002791-tqchfdmy	179	7	verified	verify	VBN
cord-002791-tqchfdmy	179	8	by	by	IN
cord-002791-tqchfdmy	179	9	TEM	TEM	NNP
cord-002791-tqchfdmy	179	10	using	use	VBG
cord-002791-tqchfdmy	179	11	a	a	DT
cord-002791-tqchfdmy	179	12	Philips	Philips	NNP
cord-002791-tqchfdmy	179	13	EM	EM	NNP
cord-002791-tqchfdmy	179	14	208	208	CD
cord-002791-tqchfdmy	179	15	electron	electron	NN
cord-002791-tqchfdmy	179	16	microscope	microscope	NN
cord-002791-tqchfdmy	179	17	(	(	-LRB-
cord-002791-tqchfdmy	179	18	FEI	FEI	NNP
cord-002791-tqchfdmy	179	19	,	,	,
cord-002791-tqchfdmy	179	20	Czech	Czech	NNP
cord-002791-tqchfdmy	179	21	Republic	Republic	NNP
cord-002791-tqchfdmy	179	22	)	)	-RRB-
cord-002791-tqchfdmy	179	23	.	.	.
cord-002791-tqchfdmy	180	1	The	the	DT
cord-002791-tqchfdmy	180	2	sample	sample	NN
cord-002791-tqchfdmy	180	3	of	of	IN
cord-002791-tqchfdmy	180	4	supernatant	supernatant	JJ
cord-002791-tqchfdmy	180	5	containing	contain	VBG
cord-002791-tqchfdmy	180	6	His	-PRON-	PRP$
cord-002791-tqchfdmy	180	7	-	-	HYPH
cord-002791-tqchfdmy	180	8	tagged	tag	VBN
cord-002791-tqchfdmy	180	9	MS2	MS2	NNP
cord-002791-tqchfdmy	180	10	PLP	PLP	NNP
cord-002791-tqchfdmy	180	11	was	be	VBD
cord-002791-tqchfdmy	180	12	applied	apply	VBN
cord-002791-tqchfdmy	180	13	to	to	IN
cord-002791-tqchfdmy	180	14	the	the	DT
cord-002791-tqchfdmy	180	15	grid	grid	NN
cord-002791-tqchfdmy	180	16	,	,	,
cord-002791-tqchfdmy	180	17	stained	stain	VBN
cord-002791-tqchfdmy	180	18	with	with	IN
cord-002791-tqchfdmy	180	19	2	2	CD
cord-002791-tqchfdmy	180	20	%	%	NN
cord-002791-tqchfdmy	180	21	ammonium	ammonium	NN
cord-002791-tqchfdmy	180	22	molybdate	molybdate	NN
cord-002791-tqchfdmy	180	23	(	(	-LRB-
cord-002791-tqchfdmy	180	24	pH	pH	NNP
cord-002791-tqchfdmy	180	25	=	=	NNP
cord-002791-tqchfdmy	180	26	7.0	7.0	CD
cord-002791-tqchfdmy	180	27	)	)	-RRB-
cord-002791-tqchfdmy	180	28	for	for	IN
cord-002791-tqchfdmy	180	29	1	1	CD
cord-002791-tqchfdmy	180	30	minute	minute	NN
cord-002791-tqchfdmy	180	31	,	,	,
cord-002791-tqchfdmy	180	32	and	and	CC
cord-002791-tqchfdmy	180	33	inspected	inspect	VBN
cord-002791-tqchfdmy	180	34	at	at	IN
cord-002791-tqchfdmy	180	35	18,000	18,000	CD
cord-002791-tqchfdmy	180	36	×	×	NN
cord-002791-tqchfdmy	180	37	magnification	magnification	NN
cord-002791-tqchfdmy	180	38	and	and	CC
cord-002791-tqchfdmy	180	39	an	an	DT
cord-002791-tqchfdmy	180	40	accelerating	accelerate	VBG
cord-002791-tqchfdmy	180	41	voltage	voltage	NN
cord-002791-tqchfdmy	180	42	of	of	IN
cord-002791-tqchfdmy	180	43	80	80	CD
cord-002791-tqchfdmy	180	44	kV.	kv.	NN
cord-002791-tqchfdmy	180	45	Purified	purify	VBN
cord-002791-tqchfdmy	181	1	His	-PRON-	PRP$
cord-002791-tqchfdmy	181	2	-	-	HYPH
cord-002791-tqchfdmy	181	3	tagged	tag	VBN
cord-002791-tqchfdmy	181	4	MS2	MS2	NNP
cord-002791-tqchfdmy	181	5	PLP	PLP	NNP
cord-002791-tqchfdmy	181	6	were	be	VBD
cord-002791-tqchfdmy	181	7	quantified	quantify	VBN
cord-002791-tqchfdmy	181	8	by	by	IN
cord-002791-tqchfdmy	181	9	RT	RT	NNP
cord-002791-tqchfdmy	181	10	-	-	HYPH
cord-002791-tqchfdmy	181	11	qPCR	qPCR	NNP
cord-002791-tqchfdmy	181	12	as	as	IN
cord-002791-tqchfdmy	181	13	was	be	VBD
cord-002791-tqchfdmy	181	14	described	describe	VBN
cord-002791-tqchfdmy	181	15	previously	previously	RB
cord-002791-tqchfdmy	181	16	8	8	CD
cord-002791-tqchfdmy	181	17	.	.	.
cord-002791-tqchfdmy	182	1	The	the	DT
cord-002791-tqchfdmy	182	2	quantity	quantity	NN
cord-002791-tqchfdmy	182	3	of	of	IN
cord-002791-tqchfdmy	182	4	FPLC	FPLC	NNP
cord-002791-tqchfdmy	182	5	-	-	HYPH
cord-002791-tqchfdmy	182	6	purified	purify	VBN
cord-002791-tqchfdmy	182	7	His	-PRON-	PRP$
cord-002791-tqchfdmy	182	8	-	-	HYPH
cord-002791-tqchfdmy	182	9	tagged	tag	VBN
cord-002791-tqchfdmy	182	10	MS2	MS2	NNP
cord-002791-tqchfdmy	182	11	PLP	PLP	NNP
cord-002791-tqchfdmy	182	12	was	be	VBD
cord-002791-tqchfdmy	182	13	compared	compare	VBN
cord-002791-tqchfdmy	182	14	with	with	IN
cord-002791-tqchfdmy	182	15	previously	previously	RB
cord-002791-tqchfdmy	182	16	produced	produce	VBN
cord-002791-tqchfdmy	182	17	non	non	JJ
cord-002791-tqchfdmy	182	18	-	-	JJ
cord-002791-tqchfdmy	182	19	His	-PRON-	PRP$
cord-002791-tqchfdmy	182	20	-	-	HYPH
cord-002791-tqchfdmy	182	21	tagged	tag	VBN
cord-002791-tqchfdmy	182	22	MS2	MS2	NNP
cord-002791-tqchfdmy	182	23	PLP	PLP	NNP
cord-002791-tqchfdmy	182	24	purified	purify	VBN
cord-002791-tqchfdmy	182	25	through	through	IN
cord-002791-tqchfdmy	182	26	ultracentrifugation	ultracentrifugation	NN
cord-002791-tqchfdmy	182	27	8	8	CD
cord-002791-tqchfdmy	182	28	.	.	.
cord-002791-tqchfdmy	183	1	To	to	TO
cord-002791-tqchfdmy	183	2	confirm	confirm	VB
cord-002791-tqchfdmy	183	3	the	the	DT
cord-002791-tqchfdmy	183	4	production	production	NN
cord-002791-tqchfdmy	183	5	of	of	IN
cord-002791-tqchfdmy	183	6	the	the	DT
cord-002791-tqchfdmy	183	7	single	single	JJ
cord-002791-tqchfdmy	183	8	-	-	HYPH
cord-002791-tqchfdmy	183	9	chain	chain	NN
cord-002791-tqchfdmy	183	10	version	version	NN
cord-002791-tqchfdmy	183	11	of	of	IN
cord-002791-tqchfdmy	183	12	the	the	DT
cord-002791-tqchfdmy	183	13	coat	coat	NN
cord-002791-tqchfdmy	183	14	protein	protein	NN
cord-002791-tqchfdmy	183	15	dimer	dimer	NN
cord-002791-tqchfdmy	183	16	containing	contain	VBG
cord-002791-tqchfdmy	183	17	the	the	DT
cord-002791-tqchfdmy	183	18	His	-PRON-	PRP$
cord-002791-tqchfdmy	183	19	-	-	HYPH
cord-002791-tqchfdmy	183	20	tag	tag	NN
cord-002791-tqchfdmy	183	21	,	,	,
cord-002791-tqchfdmy	183	22	SDS	SDS	NNP
cord-002791-tqchfdmy	183	23	-	-	HYPH
cord-002791-tqchfdmy	183	24	PAGE	PAGE	NNP
cord-002791-tqchfdmy	183	25	,	,	,
cord-002791-tqchfdmy	183	26	western	western	JJ
cord-002791-tqchfdmy	183	27	blot	blot	NN
cord-002791-tqchfdmy	183	28	analysis	analysis	NN
cord-002791-tqchfdmy	183	29	,	,	,
cord-002791-tqchfdmy	183	30	and	and	CC
cord-002791-tqchfdmy	183	31	MALDI	MALDI	NNP
cord-002791-tqchfdmy	183	32	-	-	HYPH
cord-002791-tqchfdmy	183	33	TOF	TOF	NNP
cord-002791-tqchfdmy	183	34	analysis	analysis	NN
cord-002791-tqchfdmy	183	35	were	be	VBD
cord-002791-tqchfdmy	183	36	applied	apply	VBN
cord-002791-tqchfdmy	183	37	.	.	.
cord-002791-tqchfdmy	184	1	As	as	IN
cord-002791-tqchfdmy	184	2	a	a	DT
cord-002791-tqchfdmy	184	3	control	control	NN
cord-002791-tqchfdmy	184	4	material	material	NN
cord-002791-tqchfdmy	184	5	,	,	,
cord-002791-tqchfdmy	184	6	previously	previously	RB
cord-002791-tqchfdmy	184	7	produced	produce	VBD
cord-002791-tqchfdmy	184	8	MS2	MS2	NNP
cord-002791-tqchfdmy	184	9	PLP	PLP	NNP
cord-002791-tqchfdmy	184	10	without	without	IN
cord-002791-tqchfdmy	184	11	His	-PRON-	PRP$
cord-002791-tqchfdmy	184	12	-	-	HYPH
cord-002791-tqchfdmy	184	13	tag	tag	NN
cord-002791-tqchfdmy	184	14	,	,	,
cord-002791-tqchfdmy	184	15	composed	compose	VBN
cord-002791-tqchfdmy	184	16	of	of	IN
cord-002791-tqchfdmy	184	17	wild	wild	JJ
cord-002791-tqchfdmy	184	18	-	-	HYPH
cord-002791-tqchfdmy	184	19	type	type	NN
cord-002791-tqchfdmy	184	20	MS2	MS2	NNP
cord-002791-tqchfdmy	184	21	bacteriophage	bacteriophage	NN
cord-002791-tqchfdmy	184	22	coat	coat	NN
cord-002791-tqchfdmy	184	23	protein	protein	NNP
cord-002791-tqchfdmy	184	24	8	8	CD
cord-002791-tqchfdmy	184	25	,	,	,
cord-002791-tqchfdmy	184	26	were	be	VBD
cord-002791-tqchfdmy	184	27	used	use	VBN
cord-002791-tqchfdmy	184	28	.	.	.
cord-002791-tqchfdmy	185	1	Briefly	briefly	RB
cord-002791-tqchfdmy	185	2	,	,	,
cord-002791-tqchfdmy	185	3	MS2	MS2	NNP
cord-002791-tqchfdmy	185	4	PLP	PLP	NNP
cord-002791-tqchfdmy	185	5	and	and	CC
cord-002791-tqchfdmy	185	6	His	-PRON-	PRP$
cord-002791-tqchfdmy	185	7	-	-	HYPH
cord-002791-tqchfdmy	185	8	tagged	tag	VBN
cord-002791-tqchfdmy	185	9	MS2	MS2	NNP
cord-002791-tqchfdmy	185	10	PLP	PLP	NNP
cord-002791-tqchfdmy	185	11	were	be	VBD
cord-002791-tqchfdmy	185	12	resolved	resolve	VBN
cord-002791-tqchfdmy	185	13	by	by	IN
cord-002791-tqchfdmy	185	14	SDS	SDS	NNP
cord-002791-tqchfdmy	185	15	-	-	HYPH
cord-002791-tqchfdmy	185	16	PAGE	PAGE	NNP
cord-002791-tqchfdmy	185	17	31	31	CD
cord-002791-tqchfdmy	185	18	using	use	VBG
cord-002791-tqchfdmy	185	19	a	a	DT
cord-002791-tqchfdmy	185	20	15	15	CD
cord-002791-tqchfdmy	185	21	%	%	NN
cord-002791-tqchfdmy	185	22	separating	separate	VBG
cord-002791-tqchfdmy	185	23	polyacrylamide	polyacrylamide	NN
cord-002791-tqchfdmy	185	24	gel	gel	NN
cord-002791-tqchfdmy	185	25	and	and	CC
cord-002791-tqchfdmy	185	26	transferred	transfer	VBN
cord-002791-tqchfdmy	185	27	to	to	IN
cord-002791-tqchfdmy	185	28	polyvinylidene	polyvinylidene	NN
cord-002791-tqchfdmy	185	29	difluoride	difluoride	NN
cord-002791-tqchfdmy	185	30	membranes	membrane	NNS
cord-002791-tqchfdmy	185	31	(	(	-LRB-
cord-002791-tqchfdmy	185	32	PVDF	PVDF	NNP
cord-002791-tqchfdmy	185	33	;	;	:
cord-002791-tqchfdmy	185	34	Amersham	Amersham	NNP
cord-002791-tqchfdmy	185	35	,	,	,
cord-002791-tqchfdmy	185	36	UK	UK	NNP
cord-002791-tqchfdmy	185	37	)	)	-RRB-
cord-002791-tqchfdmy	185	38	which	which	WDT
cord-002791-tqchfdmy	185	39	were	be	VBD
cord-002791-tqchfdmy	185	40	subsequently	subsequently	RB
cord-002791-tqchfdmy	185	41	blocked	block	VBN
cord-002791-tqchfdmy	185	42	in	in	IN
cord-002791-tqchfdmy	185	43	1	1	CD
cord-002791-tqchfdmy	185	44	%	%	NN
cord-002791-tqchfdmy	185	45	casein	casein	NN
cord-002791-tqchfdmy	185	46	hydrolysate	hydrolysate	NN
cord-002791-tqchfdmy	185	47	(	(	-LRB-
cord-002791-tqchfdmy	185	48	Imuna	Imuna	NNP
cord-002791-tqchfdmy	185	49	,	,	,
cord-002791-tqchfdmy	185	50	Czech	Czech	NNP
cord-002791-tqchfdmy	185	51	Republic	Republic	NNP
cord-002791-tqchfdmy	185	52	)	)	-RRB-
cord-002791-tqchfdmy	185	53	overnight	overnight	RB
cord-002791-tqchfdmy	185	54	at	at	IN
cord-002791-tqchfdmy	185	55	4	4	CD
cord-002791-tqchfdmy	185	56	°	°	NN
cord-002791-tqchfdmy	185	57	C	c	NN
cord-002791-tqchfdmy	185	58	.	.	.
cord-002791-tqchfdmy	186	1	Protein	protein	NN
cord-002791-tqchfdmy	186	2	bands	band	NNS
cord-002791-tqchfdmy	186	3	resolved	resolve	VBN
cord-002791-tqchfdmy	186	4	by	by	IN
cord-002791-tqchfdmy	186	5	SDS	SDS	NNP
cord-002791-tqchfdmy	186	6	-	-	HYPH
cord-002791-tqchfdmy	186	7	PAGE	PAGE	NNP
cord-002791-tqchfdmy	186	8	were	be	VBD
cord-002791-tqchfdmy	186	9	visualized	visualize	VBN
cord-002791-tqchfdmy	186	10	by	by	IN
cord-002791-tqchfdmy	186	11	staining	stain	VBG
cord-002791-tqchfdmy	186	12	with	with	IN
cord-002791-tqchfdmy	186	13	Coomassie	Coomassie	NNP
cord-002791-tqchfdmy	186	14	brilliant	brilliant	JJ
cord-002791-tqchfdmy	186	15	blue	blue	NN
cord-002791-tqchfdmy	186	16	R-250	r-250	NN
cord-002791-tqchfdmy	186	17	.	.	.
cord-002791-tqchfdmy	187	1	The	the	DT
cord-002791-tqchfdmy	187	2	membranes	membrane	NNS
cord-002791-tqchfdmy	187	3	were	be	VBD
cord-002791-tqchfdmy	187	4	incubated	incubate	VBN
cord-002791-tqchfdmy	187	5	with	with	IN
cord-002791-tqchfdmy	187	6	Anti	Anti	NNP
cord-002791-tqchfdmy	187	7	-	-	NNP
cord-002791-tqchfdmy	187	8	Enterobacterio	Enterobacterio	NNP
cord-002791-tqchfdmy	187	9	Phage	Phage	NNP
cord-002791-tqchfdmy	187	10	MS2	MS2	NNP
cord-002791-tqchfdmy	187	11	Coat	Coat	NNP
cord-002791-tqchfdmy	187	12	Protein	Protein	NNP
cord-002791-tqchfdmy	187	13	polyclonal	polyclonal	JJ
cord-002791-tqchfdmy	187	14	antibody	antibody	NN
cord-002791-tqchfdmy	187	15	(	(	-LRB-
cord-002791-tqchfdmy	187	16	Merck	Merck	NNP
cord-002791-tqchfdmy	187	17	Millipore	Millipore	NNP
cord-002791-tqchfdmy	187	18	,	,	,
cord-002791-tqchfdmy	187	19	USA	USA	NNP
cord-002791-tqchfdmy	187	20	)	)	-RRB-
cord-002791-tqchfdmy	187	21	at	at	IN
cord-002791-tqchfdmy	187	22	a	a	DT
cord-002791-tqchfdmy	187	23	dilution	dilution	NN
cord-002791-tqchfdmy	187	24	of	of	IN
cord-002791-tqchfdmy	187	25	1:1500	1:1500	CD
cord-002791-tqchfdmy	187	26	for	for	IN
cord-002791-tqchfdmy	187	27	1	1	CD
cord-002791-tqchfdmy	187	28	hour	hour	NN
cord-002791-tqchfdmy	187	29	at	at	IN
cord-002791-tqchfdmy	187	30	room	room	NN
cord-002791-tqchfdmy	187	31	temperature	temperature	NN
cord-002791-tqchfdmy	187	32	,	,	,
cord-002791-tqchfdmy	187	33	washed	wash	VBN
cord-002791-tqchfdmy	187	34	in	in	IN
cord-002791-tqchfdmy	187	35	PBS	PBS	NNP
cord-002791-tqchfdmy	187	36	with	with	IN
cord-002791-tqchfdmy	187	37	0.05	0.05	CD
cord-002791-tqchfdmy	187	38	%	%	NN
cord-002791-tqchfdmy	187	39	Tween-20	Tween-20	NNP
cord-002791-tqchfdmy	187	40	(	(	-LRB-
cord-002791-tqchfdmy	187	41	PBS	PBS	NNP
cord-002791-tqchfdmy	187	42	/	/	SYM
cord-002791-tqchfdmy	187	43	T	T	NNP
cord-002791-tqchfdmy	187	44	,	,	,
cord-002791-tqchfdmy	187	45	Serva	Serva	NNP
cord-002791-tqchfdmy	187	46	,	,	,
cord-002791-tqchfdmy	187	47	Germany	Germany	NNP
cord-002791-tqchfdmy	187	48	)	)	-RRB-
cord-002791-tqchfdmy	187	49	,	,	,
cord-002791-tqchfdmy	187	50	and	and	CC
cord-002791-tqchfdmy	187	51	then	then	RB
cord-002791-tqchfdmy	187	52	incubated	incubate	VBD
cord-002791-tqchfdmy	187	53	for	for	IN
cord-002791-tqchfdmy	187	54	1	1	CD
cord-002791-tqchfdmy	187	55	hour	hour	NN
cord-002791-tqchfdmy	187	56	at	at	IN
cord-002791-tqchfdmy	187	57	room	room	NN
cord-002791-tqchfdmy	187	58	temperature	temperature	NN
cord-002791-tqchfdmy	187	59	with	with	IN
cord-002791-tqchfdmy	187	60	goat	goat	NN
cord-002791-tqchfdmy	187	61	anti	anti	JJ
cord-002791-tqchfdmy	187	62	-	-	JJ
cord-002791-tqchfdmy	187	63	rabbit	rabbit	JJ
cord-002791-tqchfdmy	187	64	IgG	igg	NN
cord-002791-tqchfdmy	187	65	conjugated	conjugate	VBN
cord-002791-tqchfdmy	187	66	with	with	IN
cord-002791-tqchfdmy	187	67	HRP	HRP	NNP
cord-002791-tqchfdmy	187	68	diluted	dilute	VBN
cord-002791-tqchfdmy	187	69	1:1500	1:1500	CD
cord-002791-tqchfdmy	188	1	(	(	-LRB-
cord-002791-tqchfdmy	188	2	Jackson	Jackson	NNP
cord-002791-tqchfdmy	188	3	ImmunoResearch	ImmunoResearch	NNP
cord-002791-tqchfdmy	188	4	,	,	,
cord-002791-tqchfdmy	188	5	UK	UK	NNP
cord-002791-tqchfdmy	188	6	)	)	-RRB-
cord-002791-tqchfdmy	188	7	.	.	.
cord-002791-tqchfdmy	189	1	After	after	IN
cord-002791-tqchfdmy	189	2	a	a	DT
cord-002791-tqchfdmy	189	3	washing	washing	NN
cord-002791-tqchfdmy	189	4	step	step	NN
cord-002791-tqchfdmy	189	5	,	,	,
cord-002791-tqchfdmy	189	6	the	the	DT
cord-002791-tqchfdmy	189	7	protein	protein	NN
cord-002791-tqchfdmy	189	8	bands	band	NNS
cord-002791-tqchfdmy	189	9	were	be	VBD
cord-002791-tqchfdmy	189	10	visualized	visualize	VBN
cord-002791-tqchfdmy	189	11	with	with	IN
cord-002791-tqchfdmy	189	12	3,3′-diaminobenzidine	3,3′-diaminobenzidine	NNP
cord-002791-tqchfdmy	189	13	(	(	-LRB-
cord-002791-tqchfdmy	189	14	Sigma	Sigma	NNP
cord-002791-tqchfdmy	189	15	-	-	HYPH
cord-002791-tqchfdmy	189	16	Aldrich	Aldrich	NNP
cord-002791-tqchfdmy	189	17	)	)	-RRB-
cord-002791-tqchfdmy	189	18	.	.	.
cord-002791-tqchfdmy	190	1	Western	western	JJ
cord-002791-tqchfdmy	190	2	blotting	blot	VBG
cord-002791-tqchfdmy	190	3	with	with	IN
cord-002791-tqchfdmy	190	4	an	an	DT
cord-002791-tqchfdmy	190	5	anti	anti	JJ
cord-002791-tqchfdmy	190	6	-	-	JJ
cord-002791-tqchfdmy	190	7	HisTag	histag	JJ
cord-002791-tqchfdmy	190	8	antibody	antibody	NN
cord-002791-tqchfdmy	190	9	(	(	-LRB-
cord-002791-tqchfdmy	190	10	Pierce	Pierce	NNP
cord-002791-tqchfdmy	190	11	,	,	,
cord-002791-tqchfdmy	190	12	Thermo	Thermo	NNP
cord-002791-tqchfdmy	190	13	Scientific	Scientific	NNP
cord-002791-tqchfdmy	190	14	,	,	,
cord-002791-tqchfdmy	190	15	USA	USA	NNP
cord-002791-tqchfdmy	190	16	)	)	-RRB-
cord-002791-tqchfdmy	190	17	diluted	dilute	VBD
cord-002791-tqchfdmy	190	18	1:3000	1:3000	.
cord-002791-tqchfdmy	191	1	as	as	IN
cord-002791-tqchfdmy	191	2	primary	primary	JJ
cord-002791-tqchfdmy	191	3	antibody	antibody	NN
cord-002791-tqchfdmy	191	4	and	and	CC
cord-002791-tqchfdmy	191	5	goat	goat	NN
cord-002791-tqchfdmy	191	6	anti	anti	JJ
cord-002791-tqchfdmy	191	7	-	-	JJ
cord-002791-tqchfdmy	191	8	mouse	mouse	JJ
cord-002791-tqchfdmy	191	9	IgG	IgG	NNP
cord-002791-tqchfdmy	191	10	conjugated	conjugate	VBN
cord-002791-tqchfdmy	191	11	with	with	IN
cord-002791-tqchfdmy	191	12	HRP	HRP	NNP
cord-002791-tqchfdmy	191	13	diluted	dilute	VBN
cord-002791-tqchfdmy	191	14	1:1000	1:1000	CD
cord-002791-tqchfdmy	192	1	(	(	-LRB-
cord-002791-tqchfdmy	192	2	Jackson	Jackson	NNP
cord-002791-tqchfdmy	192	3	ImmunoResearch	ImmunoResearch	NNP
cord-002791-tqchfdmy	192	4	)	)	-RRB-
cord-002791-tqchfdmy	192	5	as	as	IN
cord-002791-tqchfdmy	192	6	a	a	DT
cord-002791-tqchfdmy	192	7	secondary	secondary	JJ
cord-002791-tqchfdmy	192	8	antibody	antibody	NN
cord-002791-tqchfdmy	192	9	was	be	VBD
cord-002791-tqchfdmy	192	10	performed	perform	VBN
cord-002791-tqchfdmy	192	11	as	as	IN
cord-002791-tqchfdmy	192	12	described	describe	VBN
cord-002791-tqchfdmy	192	13	above	above	RB
cord-002791-tqchfdmy	192	14	.	.	.
cord-002791-tqchfdmy	193	1	MALDI	MALDI	NNP
cord-002791-tqchfdmy	193	2	-	-	HYPH
cord-002791-tqchfdmy	193	3	TOF	TOF	NNP
cord-002791-tqchfdmy	193	4	analysis	analysis	NN
cord-002791-tqchfdmy	193	5	was	be	VBD
cord-002791-tqchfdmy	193	6	performed	perform	VBN
cord-002791-tqchfdmy	193	7	as	as	IN
cord-002791-tqchfdmy	193	8	follows	follow	VBZ
cord-002791-tqchfdmy	193	9	:	:	:
cord-002791-tqchfdmy	194	1	MS2	MS2	NNP
cord-002791-tqchfdmy	194	2	PLP	PLP	NNP
cord-002791-tqchfdmy	194	3	8	8	CD
cord-002791-tqchfdmy	194	4	and	and	CC
cord-002791-tqchfdmy	195	1	His	-PRON-	PRP$
cord-002791-tqchfdmy	195	2	-	-	HYPH
cord-002791-tqchfdmy	195	3	tagged	tag	VBN
cord-002791-tqchfdmy	195	4	MS2	MS2	NNP
cord-002791-tqchfdmy	195	5	PLP	PLP	NNP
cord-002791-tqchfdmy	195	6	were	be	VBD
cord-002791-tqchfdmy	195	7	treated	treat	VBN
cord-002791-tqchfdmy	195	8	with	with	IN
cord-002791-tqchfdmy	195	9	10	10	CD
cord-002791-tqchfdmy	195	10	%	%	NN
cord-002791-tqchfdmy	195	11	β	β	NN
cord-002791-tqchfdmy	195	12	-	-	HYPH
cord-002791-tqchfdmy	195	13	mercaptoethanol	mercaptoethanol	JJ
cord-002791-tqchfdmy	195	14	(	(	-LRB-
cord-002791-tqchfdmy	195	15	Sigma	Sigma	NNP
cord-002791-tqchfdmy	195	16	-	-	HYPH
cord-002791-tqchfdmy	195	17	Aldrich	Aldrich	NNP
cord-002791-tqchfdmy	195	18	)	)	-RRB-
cord-002791-tqchfdmy	195	19	at	at	IN
cord-002791-tqchfdmy	195	20	95	95	CD
cord-002791-tqchfdmy	195	21	°	°	NN
cord-002791-tqchfdmy	195	22	C	c	NN
cord-002791-tqchfdmy	195	23	for	for	IN
cord-002791-tqchfdmy	195	24	1	1	CD
cord-002791-tqchfdmy	195	25	minute	minute	NN
cord-002791-tqchfdmy	195	26	and	and	CC
cord-002791-tqchfdmy	195	27	precipitated	precipitate	VBD
cord-002791-tqchfdmy	195	28	at	at	IN
cord-002791-tqchfdmy	195	29	0	0	CD
cord-002791-tqchfdmy	195	30	°	°	NN
cord-002791-tqchfdmy	195	31	C	c	NN
cord-002791-tqchfdmy	195	32	for	for	IN
cord-002791-tqchfdmy	195	33	15	15	CD
cord-002791-tqchfdmy	195	34	minutes	minute	NNS
cord-002791-tqchfdmy	195	35	.	.	.
cord-002791-tqchfdmy	196	1	Precipitated	precipitate	VBN
cord-002791-tqchfdmy	196	2	proteins	protein	NNS
cord-002791-tqchfdmy	196	3	were	be	VBD
cord-002791-tqchfdmy	196	4	resuspended	resuspend	VBN
cord-002791-tqchfdmy	196	5	in	in	IN
cord-002791-tqchfdmy	196	6	1	1	CD
cord-002791-tqchfdmy	196	7	%	%	NN
cord-002791-tqchfdmy	196	8	trifluoroacetic	trifluoroacetic	JJ
cord-002791-tqchfdmy	196	9	acid	acid	NN
cord-002791-tqchfdmy	196	10	and	and	CC
cord-002791-tqchfdmy	196	11	desalted	desalt	VBD
cord-002791-tqchfdmy	196	12	using	use	VBG
cord-002791-tqchfdmy	196	13	3	3	CD
cord-002791-tqchfdmy	196	14	kDa	kDa	NNS
cord-002791-tqchfdmy	196	15	and	and	CC
cord-002791-tqchfdmy	196	16	10	10	CD
cord-002791-tqchfdmy	196	17	kDa	kDa	NNP
cord-002791-tqchfdmy	196	18	Vivacon	Vivacon	NNP
cord-002791-tqchfdmy	196	19	columns	column	NNS
cord-002791-tqchfdmy	196	20	(	(	-LRB-
cord-002791-tqchfdmy	196	21	Sartorius	Sartorius	NNP
cord-002791-tqchfdmy	196	22	)	)	-RRB-
cord-002791-tqchfdmy	196	23	.	.	.
cord-002791-tqchfdmy	197	1	MALDI	MALDI	NNP
cord-002791-tqchfdmy	197	2	-	-	HYPH
cord-002791-tqchfdmy	197	3	TOF	TOF	NNP
cord-002791-tqchfdmy	197	4	MS	MS	NNP
cord-002791-tqchfdmy	197	5	analysis	analysis	NN
cord-002791-tqchfdmy	197	6	was	be	VBD
cord-002791-tqchfdmy	197	7	carried	carry	VBN
cord-002791-tqchfdmy	197	8	out	out	RP
cord-002791-tqchfdmy	197	9	using	use	VBG
cord-002791-tqchfdmy	197	10	an	an	DT
cord-002791-tqchfdmy	197	11	Ultraflextreme	Ultraflextreme	NNP
cord-002791-tqchfdmy	197	12	instrument	instrument	NN
cord-002791-tqchfdmy	197	13	(	(	-LRB-
cord-002791-tqchfdmy	197	14	Bruker	Bruker	NNP
cord-002791-tqchfdmy	197	15	Daltonics	Daltonics	NNP
cord-002791-tqchfdmy	197	16	,	,	,
cord-002791-tqchfdmy	197	17	USA	USA	NNP
cord-002791-tqchfdmy	197	18	)	)	-RRB-
cord-002791-tqchfdmy	197	19	operated	operate	VBD
cord-002791-tqchfdmy	197	20	in	in	IN
cord-002791-tqchfdmy	197	21	linear	linear	JJ
cord-002791-tqchfdmy	197	22	positive	positive	JJ
cord-002791-tqchfdmy	197	23	detection	detection	NN
cord-002791-tqchfdmy	197	24	mode	mode	NN
cord-002791-tqchfdmy	197	25	.	.	.
cord-002791-tqchfdmy	198	1	Ferulic	ferulic	NN
cord-002791-tqchfdmy	198	2	acid	acid	NN
cord-002791-tqchfdmy	198	3	(	(	-LRB-
cord-002791-tqchfdmy	198	4	Sigma	Sigma	NNP
cord-002791-tqchfdmy	198	5	-	-	HYPH
cord-002791-tqchfdmy	198	6	Aldrich	Aldrich	NNP
cord-002791-tqchfdmy	198	7	)	)	-RRB-
cord-002791-tqchfdmy	199	1	(	(	-LRB-
cord-002791-tqchfdmy	199	2	12.5	12.5	CD
cord-002791-tqchfdmy	199	3	mg	mg	NNP
cord-002791-tqchfdmy	199	4	/	/	SYM
cord-002791-tqchfdmy	199	5	ml	ml	NN
cord-002791-tqchfdmy	199	6	in	in	IN
cord-002791-tqchfdmy	199	7	water	water	NN
cord-002791-tqchfdmy	199	8	:	:	:
cord-002791-tqchfdmy	199	9	acetonitrile	acetonitrile	NN
cord-002791-tqchfdmy	199	10	:	:	:
cord-002791-tqchfdmy	200	1	formic	formic	JJ
cord-002791-tqchfdmy	200	2	acid	acid	NN
cord-002791-tqchfdmy	200	3	,	,	,
cord-002791-tqchfdmy	200	4	50:33:17	50:33:17	CD
cord-002791-tqchfdmy	200	5	,	,	,
cord-002791-tqchfdmy	200	6	v	v	NN
cord-002791-tqchfdmy	200	7	/	/	SYM
cord-002791-tqchfdmy	200	8	v	v	NN
cord-002791-tqchfdmy	200	9	mixture	mixture	NN
cord-002791-tqchfdmy	200	10	)	)	-RRB-
cord-002791-tqchfdmy	200	11	was	be	VBD
cord-002791-tqchfdmy	200	12	used	use	VBN
cord-002791-tqchfdmy	200	13	as	as	IN
cord-002791-tqchfdmy	200	14	the	the	DT
cord-002791-tqchfdmy	200	15	MALDI	MALDI	NNP
cord-002791-tqchfdmy	200	16	matrix	matrix	NN
cord-002791-tqchfdmy	200	17	in	in	IN
cord-002791-tqchfdmy	200	18	combination	combination	NN
cord-002791-tqchfdmy	200	19	with	with	IN
cord-002791-tqchfdmy	200	20	a	a	DT
cord-002791-tqchfdmy	200	21	stainless	stainless	JJ
cord-002791-tqchfdmy	200	22	steel	steel	NN
cord-002791-tqchfdmy	200	23	sample	sample	NN
cord-002791-tqchfdmy	200	24	plate	plate	NN
cord-002791-tqchfdmy	200	25	.	.	.
cord-002791-tqchfdmy	201	1	Stability	stability	NN
cord-002791-tqchfdmy	201	2	of	of	IN
cord-002791-tqchfdmy	201	3	His	-PRON-	PRP$
cord-002791-tqchfdmy	201	4	-	-	HYPH
cord-002791-tqchfdmy	201	5	tagged	tag	VBN
cord-002791-tqchfdmy	201	6	MS2	MS2	NNP
cord-002791-tqchfdmy	201	7	PLP	PLP	NNP
cord-002791-tqchfdmy	201	8	against	against	IN
cord-002791-tqchfdmy	201	9	nucleases	nuclease	NNS
cord-002791-tqchfdmy	201	10	.	.	.
cord-002791-tqchfdmy	202	1	Stability	stability	NN
cord-002791-tqchfdmy	202	2	of	of	IN
cord-002791-tqchfdmy	202	3	His	-PRON-	PRP$
cord-002791-tqchfdmy	202	4	-	-	HYPH
cord-002791-tqchfdmy	202	5	tagged	tag	VBN
cord-002791-tqchfdmy	202	6	MS2	MS2	NNP
cord-002791-tqchfdmy	202	7	PLP	PLP	NNP
cord-002791-tqchfdmy	202	8	against	against	IN
cord-002791-tqchfdmy	202	9	nucleases	nuclease	NNS
cord-002791-tqchfdmy	202	10	was	be	VBD
cord-002791-tqchfdmy	202	11	verified	verify	VBN
cord-002791-tqchfdmy	202	12	by	by	IN
cord-002791-tqchfdmy	202	13	their	-PRON-	PRP$
cord-002791-tqchfdmy	202	14	incubation	incubation	NN
cord-002791-tqchfdmy	202	15	with	with	IN
cord-002791-tqchfdmy	202	16	the	the	DT
cord-002791-tqchfdmy	202	17	combination	combination	NN
cord-002791-tqchfdmy	202	18	of	of	IN
cord-002791-tqchfdmy	202	19	10	10	CD
cord-002791-tqchfdmy	202	20	U	u	NN
cord-002791-tqchfdmy	202	21	of	of	IN
cord-002791-tqchfdmy	202	22	Turbo	Turbo	NNP
cord-002791-tqchfdmy	202	23	DNase	DNase	NNP
cord-002791-tqchfdmy	202	24	(	(	-LRB-
cord-002791-tqchfdmy	202	25	Ambion	Ambion	NNP
cord-002791-tqchfdmy	202	26	)	)	-RRB-
cord-002791-tqchfdmy	203	1	and/or	and/or	CC
cord-002791-tqchfdmy	203	2	10	10	CD
cord-002791-tqchfdmy	203	3	U	U	NNS
cord-002791-tqchfdmy	203	4	of	of	IN
cord-002791-tqchfdmy	203	5	RNase	RNase	NNP
cord-002791-tqchfdmy	203	6	A	A	NNP
cord-002791-tqchfdmy	203	7	(	(	-LRB-
cord-002791-tqchfdmy	203	8	Qiagen	Qiagen	NNP
cord-002791-tqchfdmy	203	9	)	)	-RRB-
cord-002791-tqchfdmy	203	10	at	at	IN
cord-002791-tqchfdmy	203	11	37	37	CD
cord-002791-tqchfdmy	203	12	°	°	NN
cord-002791-tqchfdmy	203	13	C	c	NN
cord-002791-tqchfdmy	203	14	for	for	IN
cord-002791-tqchfdmy	203	15	1	1	CD
cord-002791-tqchfdmy	203	16	hour	hour	NN
cord-002791-tqchfdmy	203	17	.	.	.
cord-002791-tqchfdmy	204	1	As	as	IN
cord-002791-tqchfdmy	204	2	controls	control	NNS
cord-002791-tqchfdmy	204	3	for	for	IN
cord-002791-tqchfdmy	204	4	the	the	DT
cord-002791-tqchfdmy	204	5	reaction	reaction	NN
cord-002791-tqchfdmy	204	6	,	,	,
cord-002791-tqchfdmy	204	7	DNA	DNA	NNP
cord-002791-tqchfdmy	204	8	(	(	-LRB-
cord-002791-tqchfdmy	204	9	500	500	CD
cord-002791-tqchfdmy	204	10	ng	ng	NN
cord-002791-tqchfdmy	204	11	of	of	IN
cord-002791-tqchfdmy	204	12	purified	purify	VBN
cord-002791-tqchfdmy	204	13	PCR	PCR	NNP
cord-002791-tqchfdmy	204	14	fragment	fragment	NN
cord-002791-tqchfdmy	204	15	encoding	encode	VBG
cord-002791-tqchfdmy	204	16	the	the	DT
cord-002791-tqchfdmy	204	17	maturase	maturase	NN
cord-002791-tqchfdmy	204	18	and	and	CC
cord-002791-tqchfdmy	204	19	single	single	JJ
cord-002791-tqchfdmy	204	20	-	-	HYPH
cord-002791-tqchfdmy	204	21	chain	chain	NN
cord-002791-tqchfdmy	204	22	version	version	NN
cord-002791-tqchfdmy	204	23	of	of	IN
cord-002791-tqchfdmy	204	24	the	the	DT
cord-002791-tqchfdmy	204	25	coat	coat	NN
cord-002791-tqchfdmy	204	26	protein	protein	NN
cord-002791-tqchfdmy	204	27	dimer	dimer	NNP
cord-002791-tqchfdmy	204	28	with	with	IN
cord-002791-tqchfdmy	204	29	His	-PRON-	PRP$
cord-002791-tqchfdmy	204	30	-	-	HYPH
cord-002791-tqchfdmy	204	31	tag	tag	NN
cord-002791-tqchfdmy	204	32	modification	modification	NN
cord-002791-tqchfdmy	204	33	)	)	-RRB-
cord-002791-tqchfdmy	204	34	and	and	CC
cord-002791-tqchfdmy	204	35	RNA	RNA	NNP
cord-002791-tqchfdmy	204	36	(	(	-LRB-
cord-002791-tqchfdmy	204	37	500	500	CD
cord-002791-tqchfdmy	204	38	ng	ng	NN
cord-002791-tqchfdmy	204	39	of	of	IN
cord-002791-tqchfdmy	204	40	IAC	IAC	NNP
cord-002791-tqchfdmy	204	41	in	in	IN
cord-002791-tqchfdmy	204	42	vitro	vitro	FW
cord-002791-tqchfdmy	204	43	transcript	transcript	NN
cord-002791-tqchfdmy	204	44	8	8	CD
cord-002791-tqchfdmy	204	45	)	)	-RRB-
cord-002791-tqchfdmy	204	46	,	,	,
cord-002791-tqchfdmy	204	47	were	be	VBD
cord-002791-tqchfdmy	204	48	added	add	VBN
cord-002791-tqchfdmy	204	49	to	to	IN
cord-002791-tqchfdmy	204	50	the	the	DT
cord-002791-tqchfdmy	204	51	tested	test	VBN
cord-002791-tqchfdmy	204	52	His	-PRON-	PRP$
cord-002791-tqchfdmy	204	53	-	-	HYPH
cord-002791-tqchfdmy	204	54	tagged	tag	VBN
cord-002791-tqchfdmy	204	55	MS2	MS2	NNP
cord-002791-tqchfdmy	204	56	PLP	PLP	NNP
cord-002791-tqchfdmy	204	57	.	.	.
cord-002791-tqchfdmy	205	1	The	the	DT
cord-002791-tqchfdmy	205	2	ability	ability	NN
cord-002791-tqchfdmy	205	3	of	of	IN
cord-002791-tqchfdmy	205	4	His	-PRON-	PRP$
cord-002791-tqchfdmy	205	5	-	-	HYPH
cord-002791-tqchfdmy	205	6	tagged	tag	VBN
cord-002791-tqchfdmy	205	7	MS2	MS2	NNP
cord-002791-tqchfdmy	205	8	PLP	PLP	NNP
cord-002791-tqchfdmy	205	9	to	to	TO
cord-002791-tqchfdmy	205	10	resist	resist	VB
cord-002791-tqchfdmy	205	11	the	the	DT
cord-002791-tqchfdmy	205	12	impact	impact	NN
cord-002791-tqchfdmy	205	13	of	of	IN
cord-002791-tqchfdmy	205	14	nucleases	nuclease	NNS
cord-002791-tqchfdmy	205	15	was	be	VBD
cord-002791-tqchfdmy	205	16	determined	determine	VBN
cord-002791-tqchfdmy	205	17	using	use	VBG
cord-002791-tqchfdmy	205	18	agarose	agarose	JJ
cord-002791-tqchfdmy	205	19	gel	gel	NN
cord-002791-tqchfdmy	205	20	electrophoresis	electrophoresis	NN
cord-002791-tqchfdmy	205	21	(	(	-LRB-
cord-002791-tqchfdmy	205	22	0.8	0.8	CD
cord-002791-tqchfdmy	205	23	%	%	NN
cord-002791-tqchfdmy	205	24	)	)	-RRB-
cord-002791-tqchfdmy	205	25	.	.	.
cord-002791-tqchfdmy	206	1	Temperature	temperature	NN
cord-002791-tqchfdmy	206	2	stability	stability	NN
cord-002791-tqchfdmy	206	3	of	of	IN
cord-002791-tqchfdmy	206	4	MS2	MS2	NNP
cord-002791-tqchfdmy	206	5	PLP	PLP	NNP
cord-002791-tqchfdmy	206	6	and	and	CC
cord-002791-tqchfdmy	206	7	His	-PRON-	PRP$
cord-002791-tqchfdmy	206	8	-	-	HYPH
cord-002791-tqchfdmy	206	9	tagged	tag	VBN
cord-002791-tqchfdmy	206	10	MS2	MS2	NNP
cord-002791-tqchfdmy	206	11	PLP	PLP	NNP
cord-002791-tqchfdmy	206	12	.	.	.
cord-002791-tqchfdmy	207	1	MS2	MS2	NNP
cord-002791-tqchfdmy	207	2	PLP	PLP	NNP
cord-002791-tqchfdmy	207	3	8	8	CD
cord-002791-tqchfdmy	207	4	and	and	CC
cord-002791-tqchfdmy	207	5	His	-PRON-	PRP$
cord-002791-tqchfdmy	207	6	-	-	HYPH
cord-002791-tqchfdmy	207	7	tagged	tag	VBN
cord-002791-tqchfdmy	207	8	MS2	MS2	NNP
cord-002791-tqchfdmy	207	9	PLP	PLP	NNP
cord-002791-tqchfdmy	207	10	(	(	-LRB-
cord-002791-tqchfdmy	207	11	both	both	DT
cord-002791-tqchfdmy	208	1	7	7	CD
cord-002791-tqchfdmy	208	2	µg	µg	NN
cord-002791-tqchfdmy	208	3	,	,	,
cord-002791-tqchfdmy	208	4	~10	~10	.
cord-002791-tqchfdmy	209	1	12	12	CD
cord-002791-tqchfdmy	209	2	particles	particle	NNS
cord-002791-tqchfdmy	209	3	)	)	-RRB-
cord-002791-tqchfdmy	209	4	dissolved	dissolve	VBD
cord-002791-tqchfdmy	209	5	in	in	IN
cord-002791-tqchfdmy	209	6	STE	STE	NNP
cord-002791-tqchfdmy	209	7	buffer	buffer	NN
cord-002791-tqchfdmy	209	8	were	be	VBD
cord-002791-tqchfdmy	209	9	incubated	incubate	VBN
cord-002791-tqchfdmy	209	10	for	for	IN
cord-002791-tqchfdmy	209	11	15	15	CD
cord-002791-tqchfdmy	209	12	minutes	minute	NNS
cord-002791-tqchfdmy	209	13	and	and	CC
cord-002791-tqchfdmy	209	14	5	5	CD
cord-002791-tqchfdmy	209	15	minutes	minute	NNS
cord-002791-tqchfdmy	209	16	,	,	,
cord-002791-tqchfdmy	209	17	respectively	respectively	RB
cord-002791-tqchfdmy	209	18	,	,	,
cord-002791-tqchfdmy	209	19	at	at	IN
cord-002791-tqchfdmy	209	20	the	the	DT
cord-002791-tqchfdmy	209	21	selected	select	VBN
cord-002791-tqchfdmy	209	22	temperatures	temperature	NNS
cord-002791-tqchfdmy	209	23	.	.	.
cord-002791-tqchfdmy	210	1	The	the	DT
cord-002791-tqchfdmy	210	2	BioRad	BioRad	NNP
cord-002791-tqchfdmy	210	3	Tetrad	Tetrad	NNP
cord-002791-tqchfdmy	210	4	2	2	CD
cord-002791-tqchfdmy	210	5	Peltier	Peltier	NNP
cord-002791-tqchfdmy	210	6	Thermal	Thermal	NNP
cord-002791-tqchfdmy	210	7	Cycler	Cycler	NNP
cord-002791-tqchfdmy	210	8	(	(	-LRB-
cord-002791-tqchfdmy	210	9	BioRad	BioRad	NNP
cord-002791-tqchfdmy	210	10	,	,	,
cord-002791-tqchfdmy	210	11	USA	USA	NNP
cord-002791-tqchfdmy	210	12	)	)	-RRB-
cord-002791-tqchfdmy	210	13	temperature	temperature	NN
cord-002791-tqchfdmy	210	14	gradient	gradient	NN
cord-002791-tqchfdmy	210	15	program	program	NN
cord-002791-tqchfdmy	210	16	(	(	-LRB-
cord-002791-tqchfdmy	210	17	61	61	CD
cord-002791-tqchfdmy	210	18	°	°	NN
cord-002791-tqchfdmy	210	19	C	C	NNP
cord-002791-tqchfdmy	210	20	-70	-70	NN
cord-002791-tqchfdmy	210	21	°	°	NNP
cord-002791-tqchfdmy	210	22	C	C	NNP
cord-002791-tqchfdmy	210	23	)	)	-RRB-
cord-002791-tqchfdmy	210	24	was	be	VBD
cord-002791-tqchfdmy	210	25	used	use	VBN
cord-002791-tqchfdmy	210	26	.	.	.
cord-002791-tqchfdmy	211	1	Immediately	immediately	RB
cord-002791-tqchfdmy	211	2	after	after	IN
cord-002791-tqchfdmy	211	3	heating	heating	NN
cord-002791-tqchfdmy	211	4	,	,	,
cord-002791-tqchfdmy	211	5	the	the	DT
cord-002791-tqchfdmy	211	6	samples	sample	NNS
cord-002791-tqchfdmy	211	7	were	be	VBD
cord-002791-tqchfdmy	211	8	loaded	load	VBN
cord-002791-tqchfdmy	211	9	onto	onto	IN
cord-002791-tqchfdmy	211	10	1	1	CD
cord-002791-tqchfdmy	211	11	%	%	NN
cord-002791-tqchfdmy	211	12	agarose	agarose	NN
cord-002791-tqchfdmy	211	13	gel	gel	NN
cord-002791-tqchfdmy	211	14	and	and	CC
cord-002791-tqchfdmy	211	15	the	the	DT
cord-002791-tqchfdmy	211	16	particle	particle	NN
cord-002791-tqchfdmy	211	17	position	position	NN
cord-002791-tqchfdmy	211	18	was	be	VBD
cord-002791-tqchfdmy	211	19	visualized	visualize	VBN
cord-002791-tqchfdmy	211	20	under	under	IN
cord-002791-tqchfdmy	211	21	UV	uv	NN
cord-002791-tqchfdmy	211	22	illumination	illumination	NN
cord-002791-tqchfdmy	211	23	.	.	.
cord-002791-tqchfdmy	212	1	Determination	determination	NN
cord-002791-tqchfdmy	212	2	of	of	IN
cord-002791-tqchfdmy	212	3	optimal	optimal	JJ
cord-002791-tqchfdmy	212	4	method	method	NN
cord-002791-tqchfdmy	212	5	of	of	IN
cord-002791-tqchfdmy	212	6	thermal	thermal	JJ
cord-002791-tqchfdmy	212	7	lysis	lysis	NN
cord-002791-tqchfdmy	212	8	of	of	IN
cord-002791-tqchfdmy	212	9	His	-PRON-	PRP$
cord-002791-tqchfdmy	212	10	-	-	HYPH
cord-002791-tqchfdmy	212	11	tagged	tag	VBN
cord-002791-tqchfdmy	212	12	MS2	MS2	NNP
cord-002791-tqchfdmy	212	13	PLP	PLP	NNP
cord-002791-tqchfdmy	212	14	and	and	CC
cord-002791-tqchfdmy	212	15	comparison	comparison	NN
cord-002791-tqchfdmy	212	16	of	of	IN
cord-002791-tqchfdmy	212	17	quantity	quantity	NN
cord-002791-tqchfdmy	212	18	and	and	CC
cord-002791-tqchfdmy	212	19	purity	purity	NN
cord-002791-tqchfdmy	212	20	of	of	IN
cord-002791-tqchfdmy	212	21	MS2	MS2	NNP
cord-002791-tqchfdmy	212	22	PLP	PLP	NNP
cord-002791-tqchfdmy	212	23	and	and	CC
cord-002791-tqchfdmy	212	24	His	-PRON-	PRP$
cord-002791-tqchfdmy	212	25	-	-	HYPH
cord-002791-tqchfdmy	212	26	tagged	tag	VBN
cord-002791-tqchfdmy	212	27	MS2	MS2	NNP
cord-002791-tqchfdmy	212	28	PLP	PLP	NNP
cord-002791-tqchfdmy	212	29	.	.	.
cord-002791-tqchfdmy	213	1	Based	base	VBN
cord-002791-tqchfdmy	213	2	on	on	IN
cord-002791-tqchfdmy	213	3	the	the	DT
cord-002791-tqchfdmy	213	4	results	result	NNS
cord-002791-tqchfdmy	213	5	of	of	IN
cord-002791-tqchfdmy	213	6	temperature	temperature	NN
cord-002791-tqchfdmy	213	7	stability	stability	NN
cord-002791-tqchfdmy	213	8	testing	testing	NN
cord-002791-tqchfdmy	213	9	,	,	,
cord-002791-tqchfdmy	213	10	His	-PRON-	PRP$
cord-002791-tqchfdmy	213	11	-	-	HYPH
cord-002791-tqchfdmy	213	12	tagged	tag	VBN
cord-002791-tqchfdmy	213	13	MS2	MS2	NNP
cord-002791-tqchfdmy	213	14	PLP	PLP	NNP
cord-002791-tqchfdmy	213	15	particles	particle	NNS
cord-002791-tqchfdmy	213	16	were	be	VBD
cord-002791-tqchfdmy	213	17	diluted	dilute	VBN
cord-002791-tqchfdmy	213	18	to	to	IN
cord-002791-tqchfdmy	213	19	an	an	DT
cord-002791-tqchfdmy	213	20	approximate	approximate	JJ
cord-002791-tqchfdmy	213	21	concentration	concentration	NN
cord-002791-tqchfdmy	213	22	of	of	IN
cord-002791-tqchfdmy	213	23	10	10	CD
cord-002791-tqchfdmy	213	24	6	6	CD
cord-002791-tqchfdmy	213	25	particles/µl	particles/µl	NN
cord-002791-tqchfdmy	213	26	.	.	.
cord-002791-tqchfdmy	214	1	The	the	DT
cord-002791-tqchfdmy	214	2	influence	influence	NN
cord-002791-tqchfdmy	214	3	of	of	IN
cord-002791-tqchfdmy	214	4	lysis	lysis	NN
cord-002791-tqchfdmy	214	5	temperature	temperature	NN
cord-002791-tqchfdmy	214	6	on	on	IN
cord-002791-tqchfdmy	214	7	the	the	DT
cord-002791-tqchfdmy	214	8	exact	exact	JJ
cord-002791-tqchfdmy	214	9	quantity	quantity	NN
cord-002791-tqchfdmy	214	10	of	of	IN
cord-002791-tqchfdmy	214	11	His	-PRON-	PRP$
cord-002791-tqchfdmy	214	12	-	-	HYPH
cord-002791-tqchfdmy	214	13	tagged	tag	VBN
cord-002791-tqchfdmy	214	14	MS2	MS2	NNP
cord-002791-tqchfdmy	214	15	PLP	PLP	NNP
cord-002791-tqchfdmy	214	16	was	be	VBD
cord-002791-tqchfdmy	214	17	tested	test	VBN
cord-002791-tqchfdmy	214	18	using	use	VBG
cord-002791-tqchfdmy	214	19	a	a	DT
cord-002791-tqchfdmy	214	20	previously	previously	RB
cord-002791-tqchfdmy	214	21	described	describe	VBN
cord-002791-tqchfdmy	214	22	quantitative	quantitative	JJ
cord-002791-tqchfdmy	214	23	reverse	reverse	NN
cord-002791-tqchfdmy	214	24	transcription	transcription	NN
cord-002791-tqchfdmy	214	25	polymerase	polymerase	NN
cord-002791-tqchfdmy	214	26	chain	chain	NN
cord-002791-tqchfdmy	214	27	reaction	reaction	NN
cord-002791-tqchfdmy	214	28	(	(	-LRB-
cord-002791-tqchfdmy	214	29	RT	RT	NNP
cord-002791-tqchfdmy	214	30	-	-	HYPH
cord-002791-tqchfdmy	214	31	qPCR	qPCR	NNP
cord-002791-tqchfdmy	214	32	)	)	-RRB-
cord-002791-tqchfdmy	214	33	system	system	NN
cord-002791-tqchfdmy	214	34	8	8	CD
cord-002791-tqchfdmy	214	35	with	with	IN
cord-002791-tqchfdmy	214	36	small	small	JJ
cord-002791-tqchfdmy	214	37	modifications	modification	NNS
cord-002791-tqchfdmy	214	38	;	;	:
cord-002791-tqchfdmy	214	39	non	non	JJ
cord-002791-tqchfdmy	214	40	-	-	JJ
cord-002791-tqchfdmy	214	41	treated	treated	JJ
cord-002791-tqchfdmy	214	42	and	and	CC
cord-002791-tqchfdmy	214	43	thermally	thermally	RB
cord-002791-tqchfdmy	214	44	treated	treat	VBN
cord-002791-tqchfdmy	214	45	(	(	-LRB-
cord-002791-tqchfdmy	214	46	65	65	CD
cord-002791-tqchfdmy	214	47	°	°	NN
cord-002791-tqchfdmy	214	48	C	c	NN
cord-002791-tqchfdmy	214	49	,	,	,
cord-002791-tqchfdmy	214	50	75	75	CD
cord-002791-tqchfdmy	214	51	°	°	NN
cord-002791-tqchfdmy	214	52	C	c	NN
cord-002791-tqchfdmy	214	53	,	,	,
cord-002791-tqchfdmy	214	54	85	85	CD
cord-002791-tqchfdmy	214	55	°	°	NN
cord-002791-tqchfdmy	214	56	C	c	NN
cord-002791-tqchfdmy	214	57	and	and	CC
cord-002791-tqchfdmy	214	58	95	95	CD
cord-002791-tqchfdmy	214	59	°	°	NN
cord-002791-tqchfdmy	214	60	C	c	NN
cord-002791-tqchfdmy	214	61	for	for	IN
cord-002791-tqchfdmy	214	62	5	5	CD
cord-002791-tqchfdmy	214	63	minutes	minute	NNS
cord-002791-tqchfdmy	214	64	)	)	-RRB-
cord-002791-tqchfdmy	215	1	His	-PRON-	PRP$
cord-002791-tqchfdmy	215	2	-	-	HYPH
cord-002791-tqchfdmy	215	3	tagged	tag	VBN
cord-002791-tqchfdmy	215	4	MS2	MS2	NNP
cord-002791-tqchfdmy	215	5	PLP	PLP	NNP
cord-002791-tqchfdmy	215	6	were	be	VBD
cord-002791-tqchfdmy	215	7	added	add	VBN
cord-002791-tqchfdmy	215	8	directly	directly	RB
cord-002791-tqchfdmy	215	9	to	to	IN
cord-002791-tqchfdmy	215	10	the	the	DT
cord-002791-tqchfdmy	215	11	reverse	reverse	JJ
cord-002791-tqchfdmy	215	12	transcription	transcription	NN
cord-002791-tqchfdmy	215	13	(	(	-LRB-
cord-002791-tqchfdmy	215	14	RT	RT	NNP
cord-002791-tqchfdmy	215	15	)	)	-RRB-
cord-002791-tqchfdmy	215	16	reaction	reaction	NN
cord-002791-tqchfdmy	215	17	.	.	.
cord-002791-tqchfdmy	216	1	RT	RT	NNP
cord-002791-tqchfdmy	216	2	was	be	VBD
cord-002791-tqchfdmy	216	3	carried	carry	VBN
cord-002791-tqchfdmy	216	4	out	out	RP
cord-002791-tqchfdmy	216	5	in	in	IN
cord-002791-tqchfdmy	216	6	octuplicates	octuplicate	NNS
cord-002791-tqchfdmy	216	7	for	for	IN
cord-002791-tqchfdmy	216	8	each	each	DT
cord-002791-tqchfdmy	216	9	temperature	temperature	NN
cord-002791-tqchfdmy	216	10	and	and	CC
cord-002791-tqchfdmy	216	11	qPCR	qpcr	NN
cord-002791-tqchfdmy	216	12	was	be	VBD
cord-002791-tqchfdmy	216	13	done	do	VBN
cord-002791-tqchfdmy	216	14	in	in	IN
cord-002791-tqchfdmy	216	15	duplicate	duplicate	NN
cord-002791-tqchfdmy	216	16	for	for	IN
cord-002791-tqchfdmy	216	17	each	each	DT
cord-002791-tqchfdmy	216	18	sample	sample	NN
cord-002791-tqchfdmy	216	19	.	.	.
cord-002791-tqchfdmy	217	1	Subsequently	subsequently	RB
cord-002791-tqchfdmy	217	2	,	,	,
cord-002791-tqchfdmy	217	3	the	the	DT
cord-002791-tqchfdmy	217	4	quantity	quantity	NN
cord-002791-tqchfdmy	217	5	and	and	CC
cord-002791-tqchfdmy	217	6	purity	purity	NN
cord-002791-tqchfdmy	217	7	of	of	IN
cord-002791-tqchfdmy	217	8	His	-PRON-	PRP$
cord-002791-tqchfdmy	217	9	-	-	HYPH
cord-002791-tqchfdmy	217	10	tagged	tag	VBN
cord-002791-tqchfdmy	217	11	MS2	MS2	NNP
cord-002791-tqchfdmy	217	12	PLP	PLP	NNP
cord-002791-tqchfdmy	217	13	was	be	VBD
cord-002791-tqchfdmy	217	14	compared	compare	VBN
cord-002791-tqchfdmy	217	15	with	with	IN
cord-002791-tqchfdmy	217	16	the	the	DT
cord-002791-tqchfdmy	217	17	quantity	quantity	NN
cord-002791-tqchfdmy	217	18	and	and	CC
cord-002791-tqchfdmy	217	19	purity	purity	NN
cord-002791-tqchfdmy	217	20	of	of	IN
cord-002791-tqchfdmy	217	21	previously	previously	RB
cord-002791-tqchfdmy	217	22	produced	produce	VBN
cord-002791-tqchfdmy	217	23	MS2	MS2	NNP
cord-002791-tqchfdmy	217	24	PLP	PLP	NNP
cord-002791-tqchfdmy	217	25	8	8	CD
cord-002791-tqchfdmy	217	26	.	.	.
cord-002791-tqchfdmy	218	1	MS2	MS2	NNP
cord-002791-tqchfdmy	218	2	PLP	PLP	NNP
cord-002791-tqchfdmy	218	3	were	be	VBD
cord-002791-tqchfdmy	218	4	thermally	thermally	RB
cord-002791-tqchfdmy	218	5	lysed	lyse	VBN
cord-002791-tqchfdmy	218	6	according	accord	VBG
cord-002791-tqchfdmy	218	7	to	to	IN
cord-002791-tqchfdmy	218	8	the	the	DT
cord-002791-tqchfdmy	218	9	original	original	JJ
cord-002791-tqchfdmy	218	10	protocol	protocol	NN
cord-002791-tqchfdmy	218	11	(	(	-LRB-
cord-002791-tqchfdmy	218	12	95	95	CD
cord-002791-tqchfdmy	218	13	°	°	NN
cord-002791-tqchfdmy	218	14	C	c	NN
cord-002791-tqchfdmy	218	15	for	for	IN
cord-002791-tqchfdmy	218	16	5	5	CD
cord-002791-tqchfdmy	218	17	minutes	minute	NNS
cord-002791-tqchfdmy	218	18	)	)	-RRB-
cord-002791-tqchfdmy	218	19	,	,	,
cord-002791-tqchfdmy	218	20	while	while	IN
cord-002791-tqchfdmy	218	21	His	-PRON-	PRP$
cord-002791-tqchfdmy	218	22	-	-	HYPH
cord-002791-tqchfdmy	218	23	tagged	tag	VBN
cord-002791-tqchfdmy	218	24	MS2	MS2	NNP
cord-002791-tqchfdmy	218	25	PLP	PLP	NNP
cord-002791-tqchfdmy	218	26	were	be	VBD
cord-002791-tqchfdmy	218	27	added	add	VBN
cord-002791-tqchfdmy	218	28	directly	directly	RB
cord-002791-tqchfdmy	218	29	at	at	IN
cord-002791-tqchfdmy	218	30	the	the	DT
cord-002791-tqchfdmy	218	31	RT	RT	NNP
cord-002791-tqchfdmy	218	32	step	step	NN
cord-002791-tqchfdmy	218	33	without	without	IN
cord-002791-tqchfdmy	218	34	undergoing	undergo	VBG
cord-002791-tqchfdmy	218	35	thermal	thermal	JJ
cord-002791-tqchfdmy	218	36	lysis	lysis	NN
cord-002791-tqchfdmy	218	37	according	accord	VBG
cord-002791-tqchfdmy	218	38	to	to	IN
cord-002791-tqchfdmy	218	39	the	the	DT
cord-002791-tqchfdmy	218	40	results	result	NNS
cord-002791-tqchfdmy	218	41	of	of	IN
cord-002791-tqchfdmy	218	42	determination	determination	NN
cord-002791-tqchfdmy	218	43	of	of	IN
cord-002791-tqchfdmy	218	44	the	the	DT
cord-002791-tqchfdmy	218	45	optimal	optimal	JJ
cord-002791-tqchfdmy	218	46	method	method	NN
cord-002791-tqchfdmy	218	47	of	of	IN
cord-002791-tqchfdmy	218	48	thermal	thermal	JJ
cord-002791-tqchfdmy	218	49	lysis	lysis	NN
cord-002791-tqchfdmy	218	50	.	.	.
cord-002791-tqchfdmy	219	1	The	the	DT
cord-002791-tqchfdmy	219	2	quantity	quantity	NN
cord-002791-tqchfdmy	219	3	of	of	IN
cord-002791-tqchfdmy	219	4	tested	test	VBN
cord-002791-tqchfdmy	219	5	particles	particle	NNS
cord-002791-tqchfdmy	219	6	was	be	VBD
cord-002791-tqchfdmy	219	7	in	in	IN
cord-002791-tqchfdmy	219	8	the	the	DT
cord-002791-tqchfdmy	219	9	range	range	NN
cord-002791-tqchfdmy	219	10	of	of	IN
cord-002791-tqchfdmy	219	11	10	10	CD
cord-002791-tqchfdmy	219	12	8	8	CD
cord-002791-tqchfdmy	219	13	-10	-10	CD
cord-002791-tqchfdmy	219	14	5	5	CD
cord-002791-tqchfdmy	220	1	particles/µl	particles/µl	NNP
cord-002791-tqchfdmy	220	2	.	.	.
cord-002791-tqchfdmy	221	1	To	to	TO
cord-002791-tqchfdmy	221	2	determinate	determinate	VB
cord-002791-tqchfdmy	221	3	the	the	DT
cord-002791-tqchfdmy	221	4	precise	precise	JJ
cord-002791-tqchfdmy	221	5	amount	amount	NN
cord-002791-tqchfdmy	221	6	of	of	IN
cord-002791-tqchfdmy	221	7	contaminating	contaminate	VBG
cord-002791-tqchfdmy	221	8	DNA	dna	NN
cord-002791-tqchfdmy	221	9	in	in	IN
cord-002791-tqchfdmy	221	10	the	the	DT
cord-002791-tqchfdmy	221	11	sample	sample	NN
cord-002791-tqchfdmy	221	12	,	,	,
cord-002791-tqchfdmy	221	13	RT	RT	NNP
cord-002791-tqchfdmy	221	14	-	-	HYPH
cord-002791-tqchfdmy	221	15	qPCR	qpcr	NN
cord-002791-tqchfdmy	221	16	amplification	amplification	NN
cord-002791-tqchfdmy	221	17	of	of	IN
cord-002791-tqchfdmy	221	18	the	the	DT
cord-002791-tqchfdmy	221	19	specific	specific	JJ
cord-002791-tqchfdmy	221	20	control	control	NN
cord-002791-tqchfdmy	221	21	sequence	sequence	NN
cord-002791-tqchfdmy	221	22	was	be	VBD
cord-002791-tqchfdmy	221	23	done	do	VBN
cord-002791-tqchfdmy	221	24	using	use	VBG
cord-002791-tqchfdmy	221	25	reactions	reaction	NNS
cord-002791-tqchfdmy	221	26	with	with	IN
cord-002791-tqchfdmy	221	27	and	and	CC
cord-002791-tqchfdmy	221	28	without	without	IN
cord-002791-tqchfdmy	221	29	RT	RT	NNP
cord-002791-tqchfdmy	221	30	enzyme	enzyme	NN
cord-002791-tqchfdmy	221	31	(	(	-LRB-
cord-002791-tqchfdmy	221	32	the	the	DT
cord-002791-tqchfdmy	221	33	enzyme	enzyme	NN
cord-002791-tqchfdmy	221	34	was	be	VBD
cord-002791-tqchfdmy	221	35	replaced	replace	VBN
cord-002791-tqchfdmy	221	36	with	with	IN
cord-002791-tqchfdmy	221	37	RNase	rnase	NN
cord-002791-tqchfdmy	221	38	-	-	HYPH
cord-002791-tqchfdmy	221	39	free	free	JJ
cord-002791-tqchfdmy	221	40	H	h	NN
cord-002791-tqchfdmy	221	41	2	2	CD
cord-002791-tqchfdmy	221	42	O	o	NN
cord-002791-tqchfdmy	221	43	;	;	:
cord-002791-tqchfdmy	221	44	Top	Top	NNP
cord-002791-tqchfdmy	221	45	-	-	HYPH
cord-002791-tqchfdmy	221	46	Bio	Bio	NNP
cord-002791-tqchfdmy	221	47	,	,	,
cord-002791-tqchfdmy	221	48	Czech	Czech	NNP
cord-002791-tqchfdmy	221	49	Republic	Republic	NNP
cord-002791-tqchfdmy	221	50	)	)	-RRB-
cord-002791-tqchfdmy	221	51	.	.	.
cord-002791-tqchfdmy	222	1	RT	RT	NNP
cord-002791-tqchfdmy	222	2	was	be	VBD
cord-002791-tqchfdmy	222	3	carried	carry	VBN
cord-002791-tqchfdmy	222	4	out	out	RP
cord-002791-tqchfdmy	222	5	in	in	IN
cord-002791-tqchfdmy	222	6	octuplicates	octuplicate	NNS
cord-002791-tqchfdmy	222	7	for	for	IN
cord-002791-tqchfdmy	222	8	each	each	DT
cord-002791-tqchfdmy	222	9	set	set	NN
cord-002791-tqchfdmy	222	10	of	of	IN
cord-002791-tqchfdmy	222	11	samples	sample	NNS
cord-002791-tqchfdmy	222	12	and	and	CC
cord-002791-tqchfdmy	222	13	qPCR	qPCR	NNP
cord-002791-tqchfdmy	222	14	was	be	VBD
cord-002791-tqchfdmy	222	15	done	do	VBN
cord-002791-tqchfdmy	222	16	in	in	IN
cord-002791-tqchfdmy	222	17	duplicate	duplicate	NN
cord-002791-tqchfdmy	222	18	for	for	IN
cord-002791-tqchfdmy	222	19	each	each	DT
cord-002791-tqchfdmy	222	20	sample	sample	NN
cord-002791-tqchfdmy	222	21	.	.	.
cord-002791-tqchfdmy	223	1	The	the	DT
cord-002791-tqchfdmy	223	2	datasets	dataset	NNS
cord-002791-tqchfdmy	223	3	generated	generate	VBN
cord-002791-tqchfdmy	223	4	during	during	IN
cord-002791-tqchfdmy	223	5	and/or	and/or	CC
cord-002791-tqchfdmy	223	6	analysed	analyse	VBN
cord-002791-tqchfdmy	223	7	during	during	IN
cord-002791-tqchfdmy	223	8	the	the	DT
cord-002791-tqchfdmy	223	9	current	current	JJ
cord-002791-tqchfdmy	223	10	study	study	NN
cord-002791-tqchfdmy	223	11	are	be	VBP
cord-002791-tqchfdmy	223	12	available	available	JJ
cord-002791-tqchfdmy	223	13	from	from	IN
cord-002791-tqchfdmy	223	14	the	the	DT
cord-002791-tqchfdmy	223	15	corresponding	correspond	VBG
cord-002791-tqchfdmy	223	16	author	author	NN
cord-002791-tqchfdmy	223	17	on	on	IN
cord-002791-tqchfdmy	223	18	reasonable	reasonable	JJ
cord-002791-tqchfdmy	223	19	request	request	NN
cord-002791-tqchfdmy	223	20	.	.	.
cord-002791-tqchfdmy	224	1	Bacteriophage	Bacteriophage	NNP
cord-002791-tqchfdmy	224	2	MS2	MS2	NNP
cord-002791-tqchfdmy	224	3	-another	-another	NNP
cord-002791-tqchfdmy	224	4	RNA	RNA	NNP
cord-002791-tqchfdmy	224	5	phage	phage	NN
cord-002791-tqchfdmy	224	6	Armored	Armored	NNP
cord-002791-tqchfdmy	224	7	RNA	RNA	NNP
cord-002791-tqchfdmy	224	8	technology	technology	NN
cord-002791-tqchfdmy	224	9	for	for	IN
cord-002791-tqchfdmy	224	10	production	production	NN
cord-002791-tqchfdmy	224	11	of	of	IN
cord-002791-tqchfdmy	224	12	ribonuclease	ribonuclease	NN
cord-002791-tqchfdmy	224	13	-	-	HYPH
cord-002791-tqchfdmy	224	14	resistant	resistant	JJ
cord-002791-tqchfdmy	224	15	viral	viral	JJ
cord-002791-tqchfdmy	224	16	RNA	RNA	NNP
cord-002791-tqchfdmy	224	17	controls	control	NNS
cord-002791-tqchfdmy	224	18	and	and	CC
cord-002791-tqchfdmy	224	19	standards	standard	NNS
cord-002791-tqchfdmy	224	20	Ribonuclease	ribonuclease	NN
cord-002791-tqchfdmy	224	21	resistant	resistant	JJ
cord-002791-tqchfdmy	224	22	viral	viral	JJ
cord-002791-tqchfdmy	224	23	RNA	rna	NN
cord-002791-tqchfdmy	224	24	standards	standard	NNS
cord-002791-tqchfdmy	224	25	Encapsidation	Encapsidation	NNP
cord-002791-tqchfdmy	224	26	of	of	IN
cord-002791-tqchfdmy	224	27	heterologous	heterologous	JJ
cord-002791-tqchfdmy	224	28	RNAs	rna	NNS
cord-002791-tqchfdmy	224	29	by	by	IN
cord-002791-tqchfdmy	224	30	bacteriophage	bacteriophage	NN
cord-002791-tqchfdmy	224	31	-	-	HYPH
cord-002791-tqchfdmy	224	32	MS2	ms2	NN
cord-002791-tqchfdmy	224	33	coat	coat	NN
cord-002791-tqchfdmy	224	34	protein	protein	NN
cord-002791-tqchfdmy	225	1	Ribonuclease	ribonuclease	NN
cord-002791-tqchfdmy	225	2	-	-	HYPH
cord-002791-tqchfdmy	225	3	resistant	resistant	JJ
cord-002791-tqchfdmy	225	4	RNA	RNA	NNP
cord-002791-tqchfdmy	225	5	controls	control	NNS
cord-002791-tqchfdmy	225	6	(	(	-LRB-
cord-002791-tqchfdmy	225	7	armored	armored	JJ
cord-002791-tqchfdmy	225	8	RNA	RNA	NNP
cord-002791-tqchfdmy	225	9	)	)	-RRB-
cord-002791-tqchfdmy	225	10	for	for	IN
cord-002791-tqchfdmy	225	11	reverse	reverse	JJ
cord-002791-tqchfdmy	225	12	transcription	transcription	NN
cord-002791-tqchfdmy	225	13	-	-	HYPH
cord-002791-tqchfdmy	225	14	PCR	pcr	NN
cord-002791-tqchfdmy	225	15	,	,	,
cord-002791-tqchfdmy	225	16	branched	branched	JJ
cord-002791-tqchfdmy	225	17	DNA	dna	NN
cord-002791-tqchfdmy	225	18	,	,	,
cord-002791-tqchfdmy	225	19	and	and	CC
cord-002791-tqchfdmy	225	20	genotyping	genotype	VBG
cord-002791-tqchfdmy	225	21	assays	assay	NNS
cord-002791-tqchfdmy	225	22	for	for	IN
cord-002791-tqchfdmy	225	23	hepatitis	hepatitis	NN
cord-002791-tqchfdmy	225	24	C	C	NNP
cord-002791-tqchfdmy	225	25	virus	virus	NN
cord-002791-tqchfdmy	225	26	Highly	highly	RB
cord-002791-tqchfdmy	225	27	sensitive	sensitive	JJ
cord-002791-tqchfdmy	225	28	assay	assay	NN
cord-002791-tqchfdmy	225	29	for	for	IN
cord-002791-tqchfdmy	225	30	detection	detection	NN
cord-002791-tqchfdmy	225	31	of	of	IN
cord-002791-tqchfdmy	225	32	enterovirus	enterovirus	NN
cord-002791-tqchfdmy	225	33	in	in	IN
cord-002791-tqchfdmy	225	34	clinical	clinical	JJ
cord-002791-tqchfdmy	225	35	specimens	specimen	NNS
cord-002791-tqchfdmy	225	36	by	by	IN
cord-002791-tqchfdmy	225	37	reverse	reverse	JJ
cord-002791-tqchfdmy	225	38	transcription	transcription	NN
cord-002791-tqchfdmy	225	39	-	-	HYPH
cord-002791-tqchfdmy	225	40	PCR	pcr	NN
cord-002791-tqchfdmy	225	41	with	with	IN
cord-002791-tqchfdmy	225	42	an	an	DT
cord-002791-tqchfdmy	225	43	armored	armored	JJ
cord-002791-tqchfdmy	225	44	RNA	RNA	NNP
cord-002791-tqchfdmy	225	45	internal	internal	JJ
cord-002791-tqchfdmy	225	46	control	control	NN
cord-002791-tqchfdmy	226	1	A	a	DT
cord-002791-tqchfdmy	226	2	novel	novel	JJ
cord-002791-tqchfdmy	226	3	duplex	duplex	JJ
cord-002791-tqchfdmy	226	4	real	real	JJ
cord-002791-tqchfdmy	226	5	-	-	HYPH
cord-002791-tqchfdmy	226	6	time	time	NN
cord-002791-tqchfdmy	226	7	reverse	reverse	JJ
cord-002791-tqchfdmy	226	8	transcriptase	transcriptase	NN
cord-002791-tqchfdmy	226	9	-	-	HYPH
cord-002791-tqchfdmy	226	10	polymerase	polymerase	NN
cord-002791-tqchfdmy	226	11	chain	chain	NN
cord-002791-tqchfdmy	226	12	reaction	reaction	NN
cord-002791-tqchfdmy	226	13	assay	assay	NN
cord-002791-tqchfdmy	226	14	for	for	IN
cord-002791-tqchfdmy	226	15	the	the	DT
cord-002791-tqchfdmy	226	16	detection	detection	NN
cord-002791-tqchfdmy	226	17	of	of	IN
cord-002791-tqchfdmy	226	18	hepatitis	hepatitis	NN
cord-002791-tqchfdmy	226	19	C	C	NNP
cord-002791-tqchfdmy	226	20	viral	viral	JJ
cord-002791-tqchfdmy	226	21	RNA	RNA	NNP
cord-002791-tqchfdmy	226	22	with	with	IN
cord-002791-tqchfdmy	226	23	armored	armored	JJ
cord-002791-tqchfdmy	226	24	RNA	RNA	NNP
cord-002791-tqchfdmy	226	25	as	as	IN
cord-002791-tqchfdmy	226	26	internal	internal	JJ
cord-002791-tqchfdmy	226	27	control	control	NN
cord-002791-tqchfdmy	226	28	Preparation	preparation	NN
cord-002791-tqchfdmy	226	29	of	of	IN
cord-002791-tqchfdmy	227	1	MS2	MS2	NNP
cord-002791-tqchfdmy	227	2	Phage	Phage	NNP
cord-002791-tqchfdmy	227	3	-	-	HYPH
cord-002791-tqchfdmy	227	4	Like	Like	NNP
cord-002791-tqchfdmy	227	5	Particles	particle	NNS
cord-002791-tqchfdmy	227	6	and	and	CC
cord-002791-tqchfdmy	227	7	Their	-PRON-	PRP$
cord-002791-tqchfdmy	227	8	Use	use	NN
cord-002791-tqchfdmy	228	1	As	as	IN
cord-002791-tqchfdmy	228	2	Potential	Potential	NNP
cord-002791-tqchfdmy	228	3	Process	Process	NNP
cord-002791-tqchfdmy	228	4	Control	control	NN
cord-002791-tqchfdmy	228	5	Viruses	Viruses	NNPS
cord-002791-tqchfdmy	228	6	for	for	IN
cord-002791-tqchfdmy	228	7	Detection	detection	NN
cord-002791-tqchfdmy	228	8	and	and	CC
cord-002791-tqchfdmy	228	9	Quantification	quantification	NN
cord-002791-tqchfdmy	228	10	of	of	IN
cord-002791-tqchfdmy	228	11	Enteric	Enteric	NNP
cord-002791-tqchfdmy	228	12	RNA	RNA	NNP
cord-002791-tqchfdmy	228	13	Viruses	Viruses	NNPS
cord-002791-tqchfdmy	228	14	in	in	IN
cord-002791-tqchfdmy	228	15	Different	different	JJ
cord-002791-tqchfdmy	228	16	Matrices	matrix	NNS
cord-002791-tqchfdmy	229	1	The	the	DT
cord-002791-tqchfdmy	229	2	True	True	NNP
cord-002791-tqchfdmy	229	3	Story	Story	NNP
cord-002791-tqchfdmy	229	4	and	and	CC
cord-002791-tqchfdmy	229	5	Advantages	Advantages	NNPS
cord-002791-tqchfdmy	229	6	of	of	IN
cord-002791-tqchfdmy	229	7	RNA	RNA	NNP
cord-002791-tqchfdmy	229	8	Phage	Phage	NNP
cord-002791-tqchfdmy	229	9	Capsids	Capsids	NNP
cord-002791-tqchfdmy	229	10	as	as	IN
cord-002791-tqchfdmy	229	11	Nanotools	Nanotools	NNP
cord-002791-tqchfdmy	229	12	Methods	Methods	NNPS
cord-002791-tqchfdmy	229	13	for	for	IN
cord-002791-tqchfdmy	229	14	Preparation	Preparation	NNP
cord-002791-tqchfdmy	229	15	of	of	IN
cord-002791-tqchfdmy	229	16	MS2	MS2	NNP
cord-002791-tqchfdmy	229	17	Phage	Phage	NNP
cord-002791-tqchfdmy	229	18	-	-	HYPH
cord-002791-tqchfdmy	229	19	Like	Like	NNP
cord-002791-tqchfdmy	229	20	Particles	particle	NNS
cord-002791-tqchfdmy	229	21	and	and	CC
cord-002791-tqchfdmy	229	22	Their	-PRON-	PRP$
cord-002791-tqchfdmy	229	23	Utilization	Utilization	NNP
cord-002791-tqchfdmy	229	24	as	as	IN
cord-002791-tqchfdmy	229	25	Process	Process	NNP
cord-002791-tqchfdmy	229	26	Control	Control	NNP
cord-002791-tqchfdmy	229	27	Viruses	Viruses	NNPS
cord-002791-tqchfdmy	229	28	in	in	IN
cord-002791-tqchfdmy	229	29	RT	RT	NNP
cord-002791-tqchfdmy	229	30	-	-	HYPH
cord-002791-tqchfdmy	229	31	PCR	PCR	NNP
cord-002791-tqchfdmy	229	32	and	and	CC
cord-002791-tqchfdmy	229	33	qRT	qRT	NNP
cord-002791-tqchfdmy	229	34	-	-	HYPH
cord-002791-tqchfdmy	229	35	PCR	PCR	NNP
cord-002791-tqchfdmy	229	36	Detection	Detection	NNP
cord-002791-tqchfdmy	229	37	of	of	IN
cord-002791-tqchfdmy	229	38	RNA	RNA	NNP
cord-002791-tqchfdmy	229	39	Viruses	Viruses	NNPS
cord-002791-tqchfdmy	229	40	From	from	IN
cord-002791-tqchfdmy	229	41	Food	Food	NNP
cord-002791-tqchfdmy	229	42	Matrices	Matrices	NNPS
cord-002791-tqchfdmy	229	43	and	and	CC
cord-002791-tqchfdmy	229	44	Clinical	Clinical	NNP
cord-002791-tqchfdmy	229	45	Specimens	Specimens	NNP
cord-002791-tqchfdmy	229	46	Armored	Armored	NNP
cord-002791-tqchfdmy	229	47	Long	Long	NNP
cord-002791-tqchfdmy	229	48	RNA	RNA	NNP
cord-002791-tqchfdmy	229	49	Controls	Controls	NNPS
cord-002791-tqchfdmy	229	50	or	or	CC
cord-002791-tqchfdmy	229	51	Standards	Standards	NNPS
cord-002791-tqchfdmy	229	52	for	for	IN
cord-002791-tqchfdmy	229	53	Branched	Branched	NNP
cord-002791-tqchfdmy	229	54	DNA	DNA	NNP
cord-002791-tqchfdmy	229	55	Assay	Assay	NNP
cord-002791-tqchfdmy	229	56	for	for	IN
cord-002791-tqchfdmy	229	57	Detection	Detection	NNP
cord-002791-tqchfdmy	229	58	of	of	IN
cord-002791-tqchfdmy	229	59	Human	Human	NNP
cord-002791-tqchfdmy	229	60	Immunodeficiency	Immunodeficiency	NNP
cord-002791-tqchfdmy	229	61	Virus	Virus	NNP
cord-002791-tqchfdmy	229	62	Type	Type	NNP
cord-002791-tqchfdmy	229	63	1	1	CD
cord-002791-tqchfdmy	229	64	Fast	fast	JJ
cord-002791-tqchfdmy	229	65	preparation	preparation	NN
cord-002791-tqchfdmy	229	66	of	of	IN
cord-002791-tqchfdmy	229	67	a	a	DT
cord-002791-tqchfdmy	229	68	long	long	JJ
cord-002791-tqchfdmy	229	69	chimeric	chimeric	JJ
cord-002791-tqchfdmy	229	70	armored	armored	JJ
cord-002791-tqchfdmy	229	71	RNA	RNA	NNP
cord-002791-tqchfdmy	229	72	as	as	IN
cord-002791-tqchfdmy	229	73	controls	control	NNS
cord-002791-tqchfdmy	229	74	for	for	IN
cord-002791-tqchfdmy	229	75	external	external	JJ
cord-002791-tqchfdmy	229	76	quality	quality	NN
cord-002791-tqchfdmy	229	77	assessment	assessment	NN
cord-002791-tqchfdmy	229	78	for	for	IN
cord-002791-tqchfdmy	229	79	molecular	molecular	JJ
cord-002791-tqchfdmy	229	80	detection	detection	NN
cord-002791-tqchfdmy	229	81	of	of	IN
cord-002791-tqchfdmy	229	82	Zika	Zika	NNP
cord-002791-tqchfdmy	229	83	virus	virus	NN
cord-002791-tqchfdmy	229	84	Preparation	preparation	NN
cord-002791-tqchfdmy	229	85	of	of	IN
cord-002791-tqchfdmy	229	86	His	-PRON-	PRP$
cord-002791-tqchfdmy	229	87	-	-	HYPH
cord-002791-tqchfdmy	229	88	tagged	tagged	NNP
cord-002791-tqchfdmy	229	89	armored	armored	JJ
cord-002791-tqchfdmy	229	90	RNA	RNA	NNP
cord-002791-tqchfdmy	229	91	phage	phage	NN
cord-002791-tqchfdmy	229	92	particles	particle	NNS
cord-002791-tqchfdmy	229	93	as	as	IN
cord-002791-tqchfdmy	229	94	a	a	DT
cord-002791-tqchfdmy	229	95	control	control	NN
cord-002791-tqchfdmy	229	96	for	for	IN
cord-002791-tqchfdmy	229	97	real	real	JJ
cord-002791-tqchfdmy	229	98	-	-	HYPH
cord-002791-tqchfdmy	229	99	time	time	NN
cord-002791-tqchfdmy	229	100	reverse	reverse	NN
cord-002791-tqchfdmy	229	101	transcription	transcription	NN
cord-002791-tqchfdmy	229	102	-	-	HYPH
cord-002791-tqchfdmy	229	103	PCR	pcr	NN
cord-002791-tqchfdmy	229	104	detection	detection	NN
cord-002791-tqchfdmy	229	105	of	of	IN
cord-002791-tqchfdmy	229	106	severe	severe	JJ
cord-002791-tqchfdmy	229	107	acute	acute	JJ
cord-002791-tqchfdmy	229	108	respiratory	respiratory	JJ
cord-002791-tqchfdmy	229	109	syndrome	syndrome	NN
cord-002791-tqchfdmy	229	110	coronavirus	coronavirus	NN
cord-002791-tqchfdmy	230	1	Multiple	multiple	JJ
cord-002791-tqchfdmy	230	2	presentation	presentation	NN
cord-002791-tqchfdmy	230	3	of	of	IN
cord-002791-tqchfdmy	230	4	foreign	foreign	JJ
cord-002791-tqchfdmy	230	5	peptides	peptide	NNS
cord-002791-tqchfdmy	230	6	on	on	IN
cord-002791-tqchfdmy	230	7	the	the	DT
cord-002791-tqchfdmy	230	8	surface	surface	NN
cord-002791-tqchfdmy	230	9	of	of	IN
cord-002791-tqchfdmy	230	10	an	an	DT
cord-002791-tqchfdmy	230	11	RNA	rna	NN
cord-002791-tqchfdmy	230	12	-	-	HYPH
cord-002791-tqchfdmy	230	13	free	free	JJ
cord-002791-tqchfdmy	230	14	spherical	spherical	JJ
cord-002791-tqchfdmy	230	15	bacteriophage	bacteriophage	NN
cord-002791-tqchfdmy	230	16	capsid	capsid	NN
cord-002791-tqchfdmy	230	17	Simultaneous	Simultaneous	NNP
cord-002791-tqchfdmy	230	18	Detection	Detection	NNP
cord-002791-tqchfdmy	230	19	of	of	IN
cord-002791-tqchfdmy	230	20	Seven	seven	CD
cord-002791-tqchfdmy	230	21	Enteric	enteric	JJ
cord-002791-tqchfdmy	230	22	Viruses	virus	NNS
cord-002791-tqchfdmy	230	23	Associated	associate	VBN
cord-002791-tqchfdmy	230	24	with	with	IN
cord-002791-tqchfdmy	230	25	Acute	acute	JJ
cord-002791-tqchfdmy	230	26	Gastroenteritis	Gastroenteritis	NNP
cord-002791-tqchfdmy	230	27	by	by	IN
cord-002791-tqchfdmy	230	28	a	a	DT
cord-002791-tqchfdmy	230	29	Multiplexed	Multiplexed	NNP
cord-002791-tqchfdmy	230	30	Luminex	Luminex	NNP
cord-002791-tqchfdmy	230	31	-	-	HYPH
cord-002791-tqchfdmy	230	32	Based	Based	NNP
cord-002791-tqchfdmy	230	33	Assay	Assay	NNP
cord-002791-tqchfdmy	230	34	Co	co	NN
cord-002791-tqchfdmy	230	35	-	-	NN
cord-002791-tqchfdmy	230	36	expression	expression	NN
cord-002791-tqchfdmy	230	37	of	of	IN
cord-002791-tqchfdmy	230	38	RNA	rna	NN
cord-002791-tqchfdmy	230	39	-	-	HYPH
cord-002791-tqchfdmy	230	40	protein	protein	NN
cord-002791-tqchfdmy	230	41	complexes	complex	NNS
cord-002791-tqchfdmy	230	42	in	in	IN
cord-002791-tqchfdmy	230	43	Escherichia	Escherichia	NNP
cord-002791-tqchfdmy	230	44	coli	coli	NNS
cord-002791-tqchfdmy	230	45	and	and	CC
cord-002791-tqchfdmy	230	46	applications	application	NNS
cord-002791-tqchfdmy	230	47	to	to	IN
cord-002791-tqchfdmy	230	48	RNA	RNA	NNP
cord-002791-tqchfdmy	230	49	biology	biology	NN
cord-002791-tqchfdmy	230	50	Affinity	Affinity	NNP
cord-002791-tqchfdmy	230	51	selection	selection	NN
cord-002791-tqchfdmy	230	52	of	of	IN
cord-002791-tqchfdmy	230	53	epitope	epitope	NN
cord-002791-tqchfdmy	230	54	-	-	HYPH
cord-002791-tqchfdmy	230	55	based	base	VBN
cord-002791-tqchfdmy	230	56	vaccines	vaccine	NNS
cord-002791-tqchfdmy	230	57	using	use	VBG
cord-002791-tqchfdmy	230	58	a	a	DT
cord-002791-tqchfdmy	230	59	bacteriophage	bacteriophage	NN
cord-002791-tqchfdmy	230	60	virus	virus	NN
cord-002791-tqchfdmy	230	61	-	-	:
cord-002791-tqchfdmy	230	62	like	like	JJ
cord-002791-tqchfdmy	230	63	particle	particle	NN
cord-002791-tqchfdmy	230	64	platform	platform	NN
cord-002791-tqchfdmy	230	65	Complementation	Complementation	NNP
cord-002791-tqchfdmy	230	66	of	of	IN
cord-002791-tqchfdmy	230	67	RNA	RNA	NNP
cord-002791-tqchfdmy	230	68	binding	binding	NN
cord-002791-tqchfdmy	230	69	site	site	NN
cord-002791-tqchfdmy	230	70	mutations	mutation	NNS
cord-002791-tqchfdmy	230	71	in	in	IN
cord-002791-tqchfdmy	230	72	MS2	MS2	NNP
cord-002791-tqchfdmy	230	73	coat	coat	NN
cord-002791-tqchfdmy	230	74	protein	protein	NN
cord-002791-tqchfdmy	230	75	heterodimers	heterodimer	NNS
cord-002791-tqchfdmy	230	76	Subunit	Subunit	NNP
cord-002791-tqchfdmy	230	77	fusion	fusion	NN
cord-002791-tqchfdmy	230	78	confers	confer	VBZ
cord-002791-tqchfdmy	230	79	tolerance	tolerance	NN
cord-002791-tqchfdmy	230	80	to	to	IN
cord-002791-tqchfdmy	230	81	peptide	peptide	JJ
cord-002791-tqchfdmy	230	82	insertions	insertion	NNS
cord-002791-tqchfdmy	230	83	in	in	IN
cord-002791-tqchfdmy	230	84	a	a	DT
cord-002791-tqchfdmy	230	85	virus	virus	NN
cord-002791-tqchfdmy	230	86	coat	coat	NN
cord-002791-tqchfdmy	230	87	protein	protein	NN
cord-002791-tqchfdmy	230	88	A	a	DT
cord-002791-tqchfdmy	230	89	novel	novel	JJ
cord-002791-tqchfdmy	230	90	method	method	NN
cord-002791-tqchfdmy	230	91	to	to	TO
cord-002791-tqchfdmy	230	92	produce	produce	VB
cord-002791-tqchfdmy	230	93	armored	armored	JJ
cord-002791-tqchfdmy	230	94	double	double	RB
cord-002791-tqchfdmy	230	95	-	-	HYPH
cord-002791-tqchfdmy	230	96	stranded	strand	VBN
cord-002791-tqchfdmy	230	97	DNA	dna	NN
cord-002791-tqchfdmy	230	98	by	by	IN
cord-002791-tqchfdmy	230	99	encapsulation	encapsulation	NN
cord-002791-tqchfdmy	230	100	of	of	IN
cord-002791-tqchfdmy	230	101	MS2	MS2	NNP
cord-002791-tqchfdmy	230	102	viral	viral	JJ
cord-002791-tqchfdmy	230	103	capsids	capsid	NNS
cord-002791-tqchfdmy	230	104	External	External	NNP
cord-002791-tqchfdmy	230	105	Quality	Quality	NNP
cord-002791-tqchfdmy	230	106	Assessment	Assessment	NNP
cord-002791-tqchfdmy	230	107	for	for	IN
cord-002791-tqchfdmy	230	108	the	the	DT
cord-002791-tqchfdmy	230	109	Detection	Detection	NNP
cord-002791-tqchfdmy	230	110	of	of	IN
cord-002791-tqchfdmy	230	111	Measles	Measles	NNP
cord-002791-tqchfdmy	230	112	Virus	Virus	NNP
cord-002791-tqchfdmy	230	113	by	by	IN
cord-002791-tqchfdmy	230	114	Reverse	Reverse	NNP
cord-002791-tqchfdmy	230	115	Transcription	Transcription	NNP
cord-002791-tqchfdmy	230	116	-	-	HYPH
cord-002791-tqchfdmy	230	117	PCR	PCR	NNP
cord-002791-tqchfdmy	230	118	Using	use	VBG
cord-002791-tqchfdmy	230	119	Armored	Armored	NNP
cord-002791-tqchfdmy	230	120	RNA	RNA	NNP
cord-002791-tqchfdmy	230	121	External	External	NNP
cord-002791-tqchfdmy	230	122	Quality	Quality	NNP
cord-002791-tqchfdmy	230	123	Assessment	Assessment	NNP
cord-002791-tqchfdmy	230	124	for	for	IN
cord-002791-tqchfdmy	230	125	Avian	Avian	NNP
cord-002791-tqchfdmy	230	126	Influenza	Influenza	NNP
cord-002791-tqchfdmy	230	127	A	A	NNP
cord-002791-tqchfdmy	230	128	(	(	-LRB-
cord-002791-tqchfdmy	230	129	H7N9	H7N9	NNP
cord-002791-tqchfdmy	230	130	)	)	-RRB-
cord-002791-tqchfdmy	231	1	Virus	virus	NN
cord-002791-tqchfdmy	231	2	Detection	Detection	NNP
cord-002791-tqchfdmy	231	3	Using	use	VBG
cord-002791-tqchfdmy	231	4	Armored	Armored	NNP
cord-002791-tqchfdmy	231	5	RNA	RNA	NNP
cord-002791-tqchfdmy	231	6	5′-3′	5′-3′	CD
cord-002791-tqchfdmy	231	7	Exonucleases	Exonucleases	NNPS
cord-002791-tqchfdmy	231	8	in	in	IN
cord-002791-tqchfdmy	231	9	phosphorothioate	phosphorothioate	NN
cord-002791-tqchfdmy	231	10	-	-	HYPH
cord-002791-tqchfdmy	231	11	based	base	VBN
cord-002791-tqchfdmy	231	12	oligonucleotide	oligonucleotide	NN
cord-002791-tqchfdmy	231	13	-	-	HYPH
cord-002791-tqchfdmy	231	14	directed	direct	VBN
cord-002791-tqchfdmy	231	15	mutagenesis	mutagenesis	NN
cord-002791-tqchfdmy	231	16	Messenger	Messenger	NNP
cord-002791-tqchfdmy	231	17	RNA	RNA	NNP
cord-002791-tqchfdmy	231	18	vaccine	vaccine	NN
cord-002791-tqchfdmy	231	19	based	base	VBN
cord-002791-tqchfdmy	231	20	on	on	IN
cord-002791-tqchfdmy	231	21	recombinant	recombinant	JJ
cord-002791-tqchfdmy	231	22	MS2	MS2	NNP
cord-002791-tqchfdmy	231	23	virus	virus	NN
cord-002791-tqchfdmy	231	24	-	-	HYPH
cord-002791-tqchfdmy	231	25	like	like	JJ
cord-002791-tqchfdmy	231	26	particles	particle	NNS
cord-002791-tqchfdmy	231	27	against	against	IN
cord-002791-tqchfdmy	231	28	prostate	prostate	NN
cord-002791-tqchfdmy	231	29	cancer	cancer	NN
cord-002791-tqchfdmy	231	30	MS2	MS2	NNP
cord-002791-tqchfdmy	231	31	Viruslike	Viruslike	NNP
cord-002791-tqchfdmy	231	32	Particles	Particles	NNP
cord-002791-tqchfdmy	231	33	:	:	:
cord-002791-tqchfdmy	232	1	A	a	DT
cord-002791-tqchfdmy	232	2	Robust	robust	JJ
cord-002791-tqchfdmy	232	3	,	,	,
cord-002791-tqchfdmy	232	4	Semisynthetic	Semisynthetic	NNP
cord-002791-tqchfdmy	232	5	Targeted	Targeted	NNP
cord-002791-tqchfdmy	232	6	Drug	Drug	NNP
cord-002791-tqchfdmy	232	7	Delivery	Delivery	NNP
cord-002791-tqchfdmy	232	8	Platform	Platform	NNP
cord-002791-tqchfdmy	232	9	A	a	DT
cord-002791-tqchfdmy	232	10	new	new	JJ
cord-002791-tqchfdmy	232	11	RNA	RNA	NNP
cord-002791-tqchfdmy	232	12	vaccine	vaccine	NN
cord-002791-tqchfdmy	232	13	platform	platform	NN
cord-002791-tqchfdmy	232	14	based	base	VBN
cord-002791-tqchfdmy	232	15	on	on	IN
cord-002791-tqchfdmy	232	16	MS2	MS2	NNP
cord-002791-tqchfdmy	232	17	virus	virus	NN
cord-002791-tqchfdmy	232	18	-	-	HYPH
cord-002791-tqchfdmy	232	19	like	like	JJ
cord-002791-tqchfdmy	232	20	particles	particle	NNS
cord-002791-tqchfdmy	232	21	produced	produce	VBN
cord-002791-tqchfdmy	232	22	in	in	IN
cord-002791-tqchfdmy	232	23	Saccharomyces	Saccharomyces	NNP
cord-002791-tqchfdmy	232	24	cerevisiae	cerevisiae	VBZ
cord-002791-tqchfdmy	232	25	Novel	Novel	NNP
cord-002791-tqchfdmy	232	26	miR-122	miR-122	NNP
cord-002791-tqchfdmy	232	27	delivery	delivery	NN
cord-002791-tqchfdmy	232	28	system	system	NN
cord-002791-tqchfdmy	232	29	based	base	VBN
cord-002791-tqchfdmy	232	30	on	on	IN
cord-002791-tqchfdmy	232	31	MS2	MS2	NNP
cord-002791-tqchfdmy	232	32	virus	virus	NN
cord-002791-tqchfdmy	232	33	like	like	IN
cord-002791-tqchfdmy	232	34	particle	particle	NN
cord-002791-tqchfdmy	232	35	surface	surface	NN
cord-002791-tqchfdmy	232	36	displaying	display	VBG
cord-002791-tqchfdmy	232	37	cell	cell	NN
cord-002791-tqchfdmy	232	38	-	-	HYPH
cord-002791-tqchfdmy	232	39	penetrating	penetrate	VBG
cord-002791-tqchfdmy	232	40	peptide	peptide	NN
cord-002791-tqchfdmy	232	41	TAT	tat	NN
cord-002791-tqchfdmy	232	42	for	for	IN
cord-002791-tqchfdmy	232	43	hepatocellular	hepatocellular	JJ
cord-002791-tqchfdmy	232	44	carcinoma	carcinoma	NN
cord-002791-tqchfdmy	232	45	A	a	DT
cord-002791-tqchfdmy	232	46	novel	novel	JJ
cord-002791-tqchfdmy	232	47	delivery	delivery	NN
cord-002791-tqchfdmy	232	48	platform	platform	NN
cord-002791-tqchfdmy	232	49	based	base	VBN
cord-002791-tqchfdmy	232	50	on	on	IN
cord-002791-tqchfdmy	233	1	Bacteriophage	Bacteriophage	NNP
cord-002791-tqchfdmy	233	2	MS2	MS2	NNP
cord-002791-tqchfdmy	233	3	virus	virus	NN
cord-002791-tqchfdmy	233	4	-	-	HYPH
cord-002791-tqchfdmy	233	5	like	like	JJ
cord-002791-tqchfdmy	233	6	particles	particle	NNS
cord-002791-tqchfdmy	233	7	Structure	structure	NN
cord-002791-tqchfdmy	233	8	and	and	CC
cord-002791-tqchfdmy	233	9	stability	stability	NN
cord-002791-tqchfdmy	233	10	of	of	IN
cord-002791-tqchfdmy	233	11	icosahedral	icosahedral	JJ
cord-002791-tqchfdmy	233	12	particles	particle	NNS
cord-002791-tqchfdmy	233	13	of	of	IN
cord-002791-tqchfdmy	233	14	a	a	DT
cord-002791-tqchfdmy	233	15	covalent	covalent	JJ
cord-002791-tqchfdmy	233	16	coat	coat	NN
cord-002791-tqchfdmy	233	17	protein	protein	NN
cord-002791-tqchfdmy	233	18	dimer	dimer	NNP
cord-002791-tqchfdmy	233	19	of	of	IN
cord-002791-tqchfdmy	233	20	bacteriophage	bacteriophage	NN
cord-002791-tqchfdmy	233	21	MS2	MS2	NNP
cord-002791-tqchfdmy	233	22	Thermal	Thermal	NNP
cord-002791-tqchfdmy	233	23	Stability	Stability	NNP
cord-002791-tqchfdmy	233	24	of	of	IN
cord-002791-tqchfdmy	233	25	RNA	RNA	NNP
cord-002791-tqchfdmy	233	26	Phage	Phage	NNP
cord-002791-tqchfdmy	234	1	Virus	virus	NN
cord-002791-tqchfdmy	234	2	-	-	HYPH
cord-002791-tqchfdmy	234	3	Like	like	JJ
cord-002791-tqchfdmy	234	4	Particles	particle	NNS
cord-002791-tqchfdmy	234	5	Displaying	display	VBG
cord-002791-tqchfdmy	234	6	Foreign	Foreign	NNP
cord-002791-tqchfdmy	234	7	Peptides	Peptides	NNPS
cord-002791-tqchfdmy	234	8	Cleavage	Cleavage	NNP
cord-002791-tqchfdmy	234	9	of	of	IN
cord-002791-tqchfdmy	234	10	structural	structural	JJ
cord-002791-tqchfdmy	234	11	proteins	protein	NNS
cord-002791-tqchfdmy	234	12	during	during	IN
cord-002791-tqchfdmy	234	13	assembly	assembly	NN
cord-002791-tqchfdmy	234	14	of	of	IN
cord-002791-tqchfdmy	234	15	head	head	NN
cord-002791-tqchfdmy	234	16	of	of	IN
cord-002791-tqchfdmy	234	17	bacteriophage	bacteriophage	NN
cord-002791-tqchfdmy	234	18	-	-	HYPH
cord-002791-tqchfdmy	234	19	T4	T4	NNP
cord-002791-tqchfdmy	235	1	The	the	DT
cord-002791-tqchfdmy	235	2	authors	author	NNS
cord-002791-tqchfdmy	235	3	would	would	MD
cord-002791-tqchfdmy	235	4	like	like	VB
cord-002791-tqchfdmy	235	5	to	to	TO
cord-002791-tqchfdmy	235	6	thank	thank	VB
cord-002791-tqchfdmy	235	7	Neysan	Neysan	NNP
cord-002791-tqchfdmy	235	8	Donnelly	Donnelly	NNP
cord-002791-tqchfdmy	235	9	(	(	-LRB-
cord-002791-tqchfdmy	235	10	Clear	Clear	NNP
cord-002791-tqchfdmy	235	11	Science	Science	NNP
cord-002791-tqchfdmy	235	12	Editing	Editing	NNP
cord-002791-tqchfdmy	235	13	Services	Services	NNPS
cord-002791-tqchfdmy	235	14	,	,	,
cord-002791-tqchfdmy	235	15	Germany	Germany	NNP
cord-002791-tqchfdmy	235	16	)	)	-RRB-
cord-002791-tqchfdmy	235	17	for	for	IN
cord-002791-tqchfdmy	235	18	grammatical	grammatical	JJ
cord-002791-tqchfdmy	235	19	correction	correction	NN
cord-002791-tqchfdmy	235	20	of	of	IN
cord-002791-tqchfdmy	235	21	the	the	DT
cord-002791-tqchfdmy	235	22	manuscript	manuscript	NN
cord-002791-tqchfdmy	235	23	,	,	,
cord-002791-tqchfdmy	235	24	Radek	Radek	NNP
cord-002791-tqchfdmy	235	25	Tesarik	Tesarik	NNP
cord-002791-tqchfdmy	235	26	(	(	-LRB-
cord-002791-tqchfdmy	235	27	Veterinary	Veterinary	NNP
cord-002791-tqchfdmy	235	28	Research	Research	NNP
cord-002791-tqchfdmy	235	29	Institute	Institute	NNP
cord-002791-tqchfdmy	235	30	,	,	,
cord-002791-tqchfdmy	235	31	Czech	Czech	NNP
cord-002791-tqchfdmy	235	32	Republic	Republic	NNP
cord-002791-tqchfdmy	235	33	)	)	-RRB-
cord-002791-tqchfdmy	235	34	for	for	IN
cord-002791-tqchfdmy	235	35	help	help	NN
cord-002791-tqchfdmy	235	36	with	with	IN
cord-002791-tqchfdmy	235	37	SDS	SDS	NNP
cord-002791-tqchfdmy	235	38	-	-	HYPH
cord-002791-tqchfdmy	235	39	PAGE	PAGE	NNP
cord-002791-tqchfdmy	235	40	,	,	,
cord-002791-tqchfdmy	235	41	western	western	JJ
cord-002791-tqchfdmy	235	42	blot	blot	NN
cord-002791-tqchfdmy	235	43	analysis	analysis	NN
cord-002791-tqchfdmy	235	44	and	and	CC
cord-002791-tqchfdmy	235	45	FPLC	FPLC	NNP
cord-002791-tqchfdmy	235	46	,	,	,
cord-002791-tqchfdmy	235	47	Pavel	Pavel	NNP
cord-002791-tqchfdmy	235	48	Kulich	Kulich	NNP
cord-002791-tqchfdmy	235	49	(	(	-LRB-
cord-002791-tqchfdmy	235	50	Veterinary	Veterinary	NNP
cord-002791-tqchfdmy	235	51	Research	Research	NNP
cord-002791-tqchfdmy	235	52	Institute	Institute	NNP
cord-002791-tqchfdmy	235	53	,	,	,
cord-002791-tqchfdmy	235	54	Czech	Czech	NNP
cord-002791-tqchfdmy	235	55	Republic	Republic	NNP
cord-002791-tqchfdmy	235	56	)	)	-RRB-
cord-002791-tqchfdmy	235	57	for	for	IN
cord-002791-tqchfdmy	235	58	help	help	NN
cord-002791-tqchfdmy	235	59	with	with	IN
cord-002791-tqchfdmy	235	60	TEM	TEM	NNP
cord-002791-tqchfdmy	235	61	and	and	CC
cord-002791-tqchfdmy	235	62	Lenka	Lenka	NNP
cord-002791-tqchfdmy	235	63	Cincarova	Cincarova	NNP
cord-002791-tqchfdmy	236	1	(	(	-LRB-
cord-002791-tqchfdmy	236	2	Veterinary	Veterinary	NNP
cord-002791-tqchfdmy	236	3	Research	Research	NNP
cord-002791-tqchfdmy	236	4	Institute	Institute	NNP
cord-002791-tqchfdmy	236	5	,	,	,
cord-002791-tqchfdmy	236	6	Czech	Czech	NNP
cord-002791-tqchfdmy	236	7	Republic	Republic	NNP
cord-002791-tqchfdmy	236	8	)	)	-RRB-
cord-002791-tqchfdmy	236	9	for	for	IN
cord-002791-tqchfdmy	236	10	help	help	NN
cord-002791-tqchfdmy	236	11	with	with	IN
cord-002791-tqchfdmy	236	12	MALDI	MALDI	NNP
cord-002791-tqchfdmy	236	13	-	-	HYPH
cord-002791-tqchfdmy	236	14	TOF	TOF	NNP
cord-002791-tqchfdmy	236	15	.	.	.
cord-002791-tqchfdmy	237	1	The	the	DT
cord-002791-tqchfdmy	237	2	work	work	NN
cord-002791-tqchfdmy	237	3	was	be	VBD
cord-002791-tqchfdmy	237	4	supported	support	VBN
cord-002791-tqchfdmy	237	5	by	by	IN
cord-002791-tqchfdmy	237	6	the	the	DT
cord-002791-tqchfdmy	237	7	MA	MA	NNP
cord-002791-tqchfdmy	237	8	CR	CR	NNP
cord-002791-tqchfdmy	237	9	RO0517	RO0517	NNP
cord-002791-tqchfdmy	237	10	,	,	,
cord-002791-tqchfdmy	237	11	MI	MI	NNP
cord-002791-tqchfdmy	237	12	CR	CR	NNP
cord-002791-tqchfdmy	237	13	VI20152020044	VI20152020044	NNP
cord-002791-tqchfdmy	237	14	and	and	CC
cord-002791-tqchfdmy	237	15	MEYS	MEYS	NNP
cord-002791-tqchfdmy	237	16	CR	CR	NNP
cord-002791-tqchfdmy	238	1	NPU	NPU	NNP
cord-002791-tqchfdmy	239	1	I	-PRON-	PRP
cord-002791-tqchfdmy	239	2	program	program	VBP
cord-002791-tqchfdmy	239	3	LO1218	LO1218	NNS
cord-002791-tqchfdmy	239	4	.	.	.
cord-002791-tqchfdmy	240	1	Conceived	conceive	VBN
cord-002791-tqchfdmy	240	2	and	and	CC
cord-002791-tqchfdmy	240	3	designed	design	VBD
cord-002791-tqchfdmy	240	4	the	the	DT
cord-002791-tqchfdmy	240	5	experiments	experiment	NNS
cord-002791-tqchfdmy	240	6	:	:	:
cord-002791-tqchfdmy	240	7	P.M.	p.m.	NN
cord-002791-tqchfdmy	240	8	,	,	,
cord-002791-tqchfdmy	240	9	P.V.	P.V.	NNP
cord-002791-tqchfdmy	240	10	,	,	,
cord-002791-tqchfdmy	240	11	P.K.	P.K.	NNP
cord-002791-tqchfdmy	240	12	Performed	perform	VBD
cord-002791-tqchfdmy	240	13	the	the	DT
cord-002791-tqchfdmy	240	14	experiments	experiment	NNS
cord-002791-tqchfdmy	240	15	:	:	:
cord-002791-tqchfdmy	241	1	P.M.	P.M.	NNP
cord-002791-tqchfdmy	241	2	Data	data	NN
cord-002791-tqchfdmy	241	3	analysis	analysis	NN
cord-002791-tqchfdmy	242	1	:	:	:
cord-002791-tqchfdmy	242	2	P.M.	p.m.	NN
cord-002791-tqchfdmy	242	3	Wrote	write	VBD
cord-002791-tqchfdmy	242	4	and	and	CC
cord-002791-tqchfdmy	242	5	edited	edit	VBD
cord-002791-tqchfdmy	242	6	the	the	DT
cord-002791-tqchfdmy	242	7	paper	paper	NN
cord-002791-tqchfdmy	242	8	:	:	:
cord-002791-tqchfdmy	242	9	P.M.	p.m.	NN
cord-002791-tqchfdmy	242	10	,	,	,
cord-002791-tqchfdmy	242	11	P.V.	P.V.	NNP
cord-002791-tqchfdmy	242	12	,	,	,
cord-002791-tqchfdmy	243	1	P.K.	P.K.	NNP
cord-002791-tqchfdmy	243	2	Supplementary	supplementary	JJ
cord-002791-tqchfdmy	243	3	information	information	NN
cord-002791-tqchfdmy	243	4	accompanies	accompany	VBZ
cord-002791-tqchfdmy	243	5	this	this	DT
cord-002791-tqchfdmy	243	6	paper	paper	NN
cord-002791-tqchfdmy	243	7	at	at	IN
cord-002791-tqchfdmy	243	8	https://doi.org/10.1038/s41598-017-17951-5	https://doi.org/10.1038/s41598-017-17951-5	NNP
cord-002791-tqchfdmy	243	9	.	.	.
cord-002791-tqchfdmy	244	1	The	the	DT
cord-002791-tqchfdmy	244	2	authors	author	NNS
cord-002791-tqchfdmy	244	3	declare	declare	VBP
cord-002791-tqchfdmy	244	4	that	that	IN
cord-002791-tqchfdmy	244	5	they	-PRON-	PRP
cord-002791-tqchfdmy	244	6	have	have	VBP
cord-002791-tqchfdmy	244	7	no	no	DT
cord-002791-tqchfdmy	244	8	competing	compete	VBG
cord-002791-tqchfdmy	244	9	interests	interest	NNS
cord-002791-tqchfdmy	244	10	.	.	.
cord-002791-tqchfdmy	245	1	Publisher	publisher	NN
cord-002791-tqchfdmy	245	2	's	's	POS
cord-002791-tqchfdmy	245	3	note	note	NN
cord-002791-tqchfdmy	245	4	:	:	:
cord-002791-tqchfdmy	245	5	Springer	Springer	NNP
cord-002791-tqchfdmy	245	6	Nature	Nature	NNP
cord-002791-tqchfdmy	245	7	remains	remain	VBZ
cord-002791-tqchfdmy	245	8	neutral	neutral	JJ
cord-002791-tqchfdmy	245	9	with	with	IN
cord-002791-tqchfdmy	245	10	regard	regard	NN
cord-002791-tqchfdmy	245	11	to	to	IN
cord-002791-tqchfdmy	245	12	jurisdictional	jurisdictional	JJ
cord-002791-tqchfdmy	245	13	claims	claim	NNS
cord-002791-tqchfdmy	245	14	in	in	IN
cord-002791-tqchfdmy	245	15	published	publish	VBN
cord-002791-tqchfdmy	245	16	maps	map	NNS
cord-002791-tqchfdmy	245	17	and	and	CC
cord-002791-tqchfdmy	245	18	institutional	institutional	JJ
cord-002791-tqchfdmy	245	19	affiliations	affiliation	NNS
cord-002791-tqchfdmy	245	20	.	.	.
cord-002791-tqchfdmy	246	1	Open	open	VB
cord-002791-tqchfdmy	246	2	Access	Access	NNP
cord-002791-tqchfdmy	247	1	This	this	DT
cord-002791-tqchfdmy	247	2	article	article	NN
cord-002791-tqchfdmy	247	3	is	be	VBZ
cord-002791-tqchfdmy	247	4	licensed	license	VBN
cord-002791-tqchfdmy	247	5	under	under	IN
cord-002791-tqchfdmy	247	6	a	a	DT
cord-002791-tqchfdmy	247	7	Creative	Creative	NNP
cord-002791-tqchfdmy	247	8	Commons	Commons	NNP
cord-002791-tqchfdmy	247	9	Attribution	Attribution	NNP
cord-002791-tqchfdmy	247	10	4.0	4.0	CD
cord-002791-tqchfdmy	247	11	International	International	NNP
cord-002791-tqchfdmy	247	12	License	License	NNP
cord-002791-tqchfdmy	247	13	,	,	,
cord-002791-tqchfdmy	247	14	which	which	WDT
cord-002791-tqchfdmy	247	15	permits	permit	VBZ
cord-002791-tqchfdmy	247	16	use	use	NN
cord-002791-tqchfdmy	247	17	,	,	,
cord-002791-tqchfdmy	247	18	sharing	sharing	NN
cord-002791-tqchfdmy	247	19	,	,	,
cord-002791-tqchfdmy	247	20	adaptation	adaptation	NN
cord-002791-tqchfdmy	247	21	,	,	,
cord-002791-tqchfdmy	247	22	distribution	distribution	NN
cord-002791-tqchfdmy	247	23	and	and	CC
cord-002791-tqchfdmy	247	24	reproduction	reproduction	NN
cord-002791-tqchfdmy	247	25	in	in	IN
cord-002791-tqchfdmy	247	26	any	any	DT
cord-002791-tqchfdmy	247	27	medium	medium	NN
cord-002791-tqchfdmy	247	28	or	or	CC
cord-002791-tqchfdmy	247	29	format	format	NN
cord-002791-tqchfdmy	247	30	,	,	,
cord-002791-tqchfdmy	247	31	as	as	RB
cord-002791-tqchfdmy	247	32	long	long	RB
cord-002791-tqchfdmy	247	33	as	as	IN
cord-002791-tqchfdmy	247	34	you	-PRON-	PRP
cord-002791-tqchfdmy	247	35	give	give	VBP
cord-002791-tqchfdmy	247	36	appropriate	appropriate	JJ
cord-002791-tqchfdmy	247	37	credit	credit	NN
cord-002791-tqchfdmy	247	38	to	to	IN
cord-002791-tqchfdmy	247	39	the	the	DT
cord-002791-tqchfdmy	247	40	original	original	JJ
cord-002791-tqchfdmy	247	41	author(s	author(s	NNP
cord-002791-tqchfdmy	247	42	)	)	-RRB-
cord-002791-tqchfdmy	247	43	and	and	CC
cord-002791-tqchfdmy	247	44	the	the	DT
cord-002791-tqchfdmy	247	45	source	source	NN
cord-002791-tqchfdmy	247	46	,	,	,
cord-002791-tqchfdmy	247	47	provide	provide	VB
cord-002791-tqchfdmy	247	48	a	a	DT
cord-002791-tqchfdmy	247	49	link	link	NN
cord-002791-tqchfdmy	247	50	to	to	IN
cord-002791-tqchfdmy	247	51	the	the	DT
cord-002791-tqchfdmy	247	52	Creative	Creative	NNP
cord-002791-tqchfdmy	247	53	Commons	Commons	NNP
cord-002791-tqchfdmy	247	54	license	license	NN
cord-002791-tqchfdmy	247	55	,	,	,
cord-002791-tqchfdmy	247	56	and	and	CC
cord-002791-tqchfdmy	247	57	indicate	indicate	VBP
cord-002791-tqchfdmy	247	58	if	if	IN
cord-002791-tqchfdmy	247	59	changes	change	NNS
cord-002791-tqchfdmy	247	60	were	be	VBD
cord-002791-tqchfdmy	247	61	made	make	VBN
cord-002791-tqchfdmy	247	62	.	.	.
cord-002791-tqchfdmy	248	1	The	the	DT
cord-002791-tqchfdmy	248	2	images	image	NNS
cord-002791-tqchfdmy	248	3	or	or	CC
cord-002791-tqchfdmy	248	4	other	other	JJ
cord-002791-tqchfdmy	248	5	third	third	JJ
cord-002791-tqchfdmy	248	6	party	party	NN
cord-002791-tqchfdmy	248	7	material	material	NN
cord-002791-tqchfdmy	248	8	in	in	IN
cord-002791-tqchfdmy	248	9	this	this	DT
cord-002791-tqchfdmy	248	10	article	article	NN
cord-002791-tqchfdmy	248	11	are	be	VBP
cord-002791-tqchfdmy	248	12	included	include	VBN
cord-002791-tqchfdmy	248	13	in	in	IN
cord-002791-tqchfdmy	248	14	the	the	DT
cord-002791-tqchfdmy	248	15	article	article	NN
cord-002791-tqchfdmy	248	16	's	's	POS
cord-002791-tqchfdmy	248	17	Creative	Creative	NNP
cord-002791-tqchfdmy	248	18	Commons	Commons	NNP
cord-002791-tqchfdmy	248	19	license	license	NN
cord-002791-tqchfdmy	248	20	,	,	,
cord-002791-tqchfdmy	248	21	unless	unless	IN
cord-002791-tqchfdmy	248	22	indicated	indicate	VBN
cord-002791-tqchfdmy	248	23	otherwise	otherwise	RB
cord-002791-tqchfdmy	248	24	in	in	IN
cord-002791-tqchfdmy	248	25	a	a	DT
cord-002791-tqchfdmy	248	26	credit	credit	NN
cord-002791-tqchfdmy	248	27	line	line	NN
cord-002791-tqchfdmy	248	28	to	to	IN
cord-002791-tqchfdmy	248	29	the	the	DT
cord-002791-tqchfdmy	248	30	material	material	NN
cord-002791-tqchfdmy	248	31	.	.	.
cord-002791-tqchfdmy	249	1	If	if	IN
cord-002791-tqchfdmy	249	2	material	material	NN
cord-002791-tqchfdmy	249	3	is	be	VBZ
cord-002791-tqchfdmy	249	4	not	not	RB
cord-002791-tqchfdmy	249	5	included	include	VBN
cord-002791-tqchfdmy	249	6	in	in	IN
cord-002791-tqchfdmy	249	7	the	the	DT
cord-002791-tqchfdmy	249	8	article	article	NN
cord-002791-tqchfdmy	249	9	's	's	POS
cord-002791-tqchfdmy	249	10	Creative	Creative	NNP
cord-002791-tqchfdmy	249	11	Commons	Commons	NNP
cord-002791-tqchfdmy	249	12	license	license	NN
cord-002791-tqchfdmy	249	13	and	and	CC
cord-002791-tqchfdmy	249	14	your	-PRON-	PRP$
cord-002791-tqchfdmy	249	15	intended	intend	VBN
cord-002791-tqchfdmy	249	16	use	use	NN
cord-002791-tqchfdmy	249	17	is	be	VBZ
cord-002791-tqchfdmy	249	18	not	not	RB
cord-002791-tqchfdmy	249	19	permitted	permit	VBN
cord-002791-tqchfdmy	249	20	by	by	IN
cord-002791-tqchfdmy	249	21	statutory	statutory	JJ
cord-002791-tqchfdmy	249	22	regulation	regulation	NN
cord-002791-tqchfdmy	249	23	or	or	CC
cord-002791-tqchfdmy	249	24	exceeds	exceed	VBZ
cord-002791-tqchfdmy	249	25	the	the	DT
cord-002791-tqchfdmy	249	26	permitted	permit	VBN
cord-002791-tqchfdmy	249	27	use	use	NN
cord-002791-tqchfdmy	249	28	,	,	,
cord-002791-tqchfdmy	249	29	you	-PRON-	PRP
cord-002791-tqchfdmy	249	30	will	will	MD
cord-002791-tqchfdmy	249	31	need	need	VB
cord-002791-tqchfdmy	249	32	to	to	TO
cord-002791-tqchfdmy	249	33	obtain	obtain	VB
cord-002791-tqchfdmy	249	34	permission	permission	NN
cord-002791-tqchfdmy	249	35	directly	directly	RB
cord-002791-tqchfdmy	249	36	from	from	IN
cord-002791-tqchfdmy	249	37	the	the	DT
cord-002791-tqchfdmy	249	38	copyright	copyright	NN
cord-002791-tqchfdmy	249	39	holder	holder	NN
cord-002791-tqchfdmy	249	40	.	.	.
cord-002791-tqchfdmy	250	1	To	to	TO
cord-002791-tqchfdmy	250	2	view	view	VB
cord-002791-tqchfdmy	250	3	a	a	DT
cord-002791-tqchfdmy	250	4	copy	copy	NN
cord-002791-tqchfdmy	250	5	of	of	IN
cord-002791-tqchfdmy	250	6	this	this	DT
cord-002791-tqchfdmy	250	7	license	license	NN
cord-002791-tqchfdmy	250	8	,	,	,
cord-002791-tqchfdmy	250	9	visit	visit	VB
cord-002791-tqchfdmy	250	10	http://creativecommons.org/licenses/by/4.0/.	http://creativecommons.org/licenses/by/4.0/.	NNP