id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-342676-ykog278j	Stewart, H.	Identification of novel RNA secondary structures within the hepatitis C virus genome reveals a cooperative involvement in genome packaging	2016-03-14		.txt	text/plain	6858	338	46	To identify the regions of the viral genome involved in this process, we used SELEX (systematic evolution of ligands by exponential enrichment) to identify RNA aptamers which bind specifically to Core in vitro. Comparison of these aptamers to multiple HCV genomes revealed the presence of a conserved terminal loop motif within short RNA stem-loop structures. We wished to investigate the possibility that similar specific secondary structures are present within HCV RNAs destined for packaging, and whether their interactions with Core cooperatively drive the RNA encapsidation and nucleocapsid assembly processes. A mutant genome unable to form such structures displays impaired viral infectivity whilst RNA replication is unaffected, indicating these motifs may interact specifically with Core. Role of RNA structures in genome terminal sequences of the hepatitis C virus for replication and assembly	./cache/cord-342676-ykog278j.txt	./txt/cord-342676-ykog278j.txt