id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-342289-zpstb7h9	Cui, Tingting	Establishment of porcine enterocyte/myofibroblast co-cultures for the growth of porcine rota- and coronaviruses	2018-10-12		.txt	text/plain	8364	435	49	As subepithelial myofibroblasts secrete extracellular matrix and growth factors contributing to the attachment, proliferation and differentiation of epithelial cells, co-cultures of primary porcine enterocytes (ileocytes and colonocytes) with myofibroblasts were developed and evaluated for their susceptibility to enteric viruses. The differentiation status of porcine intestinal epithelial cells of 3 days old piglets and the co-cultured enterocytes were analyzed by scanning electron microscopy. To determine the percentage of rotavirus infected cells in primary porcine enterocytes, cells were fixed at different time points (0, 3, 6, 9, 12, 24 h) post inoculation with a low-passage archival RVA strain. Therefore, the susceptibility of primary porcine enterocytes co-cultured with myofibroblasts to four different genotypes of rotavirus A contained in fecal suspensions was tested by RT-qPCR (Fig. 7) . Although rotavirus was reported to have an exclusive tropism for small intestinal enterocytes 41 , rotavirus RVA/Pig-tc/BEL/RV277/1977/G1P [7] strain could infect both primary ileum and colon epithelial cells with a trypsin treatment.	./cache/cord-342289-zpstb7h9.txt	./txt/cord-342289-zpstb7h9.txt