id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-283583-pwlbrxn3	Zhang, Xiao-Ai	Prevalence and genetic characteristics of Saffold cardiovirus in China from 2009 to 2012	2015-01-09		.txt	text/plain	3779	207	51	A higher frequency of severe clinical outcome and nervous system manifestation were also observed in the SAFV-positive HFMD patients. A patient was considered to be infected with SAFV when positive detection was obtained in any type of the samples. Genetic characterization of SAFVs. A phylogenetic tree was constructed based on the VP1 nucleotide sequences of Saffold cardiovirus detected in clinical samples of 82 patients in the current study, and sequences downloaded from GenBank by maximum likelihood method using MEGA 6.0 (Figure 1) . Detection of SAFV in sera and CSFs. Additional 171 CSFs were collected from patients with HFMD-associated encephalitis, and six (3.5%) were found to be SAFV-positive using real-time RT-PCR, and five were further confirmed by nested RT-PCR targeting the 59-UTR. In our study, SAFVs were co-detected with other viruses in 14 (82.4%), 9 (75.0%), and 28 (32.6) specimens in ARTI, diarrhea and HFMD patients respectively.	./cache/cord-283583-pwlbrxn3.txt	./txt/cord-283583-pwlbrxn3.txt