id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-005507-equnyib7	Kamath, Kathy	Antibody epitope repertoire analysis enables rapid antigen discovery and multiplex serology	2020-03-24		.txt	text/plain	5189	261	38	Given this, we developed serum epitope repertoire analysis (SERA), a method to rapidly discover conserved, pathogen-specific antigens and their epitopes, and applied it to develop an assay for Chagas disease caused by the protozoan parasite Trypanosoma cruzi. We applied serum epitope repertoire analysis (SERA) to discover shared, but highly specific immunogenic epitope motifs associated with Chagas disease caused by the protozoan parasite Trypanosoma cruzi, and thereby develop a serological assay. To identify antibody epitope motifs specific to Chagas disease, we applied the IMUNE motif discovery algorithm 15 (see Methods for parameters) to epitope repertoires from 28 Chagas specimens and 30 non-Chagas sera, yielding 331 candidate motifs (Supplementary Table S2 ). Using Chagas disease seropositive specimens, a panel of 31 peptide motifs was identified that exhibited 100% sensitivity (60/60) and 100% specificity (120/120) in independent validation sets.	./cache/cord-005507-equnyib7.txt	./txt/cord-005507-equnyib7.txt