id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-004222-z4butywi	Joyce, Collin	Comparisons of the antibody repertoires of a humanized rodent and humans by high throughput sequencing	2020-01-24		.txt	text/plain	3718	187	46	We characterized the heavy chain and kappa light chain antibody repertoires of a model animal, the OmniRat, by high throughput antibody sequencing and made use of two novel datasets for comparison to human repertoires. Multiple differences were found in both the heavy and kappa chain repertoires between OmniRats and humans including gene segment usage, CDR3 length distributions, class switch recombination, somatic hypermutation levels and in features of V(D)J recombination. We individually separated total RNA from spleens and lymph nodes of three unimmunized OmniRats and PCR amplified the heavy and kappa chain antibody V gene segments. We started by making intra-animal comparisons, intra-species comparisons and inter-species comparisons of the immunoglobulin gene segment usage frequencies for each antibody repertoire by performing hierarchical clustering ( Fig. 1 ) and linear regression analysis (Figs.	./cache/cord-004222-z4butywi.txt	./txt/cord-004222-z4butywi.txt