id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-002802-594mmlk8	Zhang, Yun	Recombinant influenza H9N2 virus with a substitution of H3 hemagglutinin transmembrane domain showed enhanced immunogenicity in mice and chicken	2017-12-20		.txt	text/plain	4269	246	47	Further, the recombinant TM-replaced H9N2-TM virus could provide better inter-clade protection in both mice and chickens against H9N2, suggesting that the H3-TM-replacement could be considered as a strategy to develop efficient subtype-specific H9N2 influenza vaccines. However, the biological characteristics, such as virus growth, ratio of trimer, thermal stability, acidic resistance and fusion activity were altered, suggesting an important role of the TM domain in viral replication and pathogenicity. Taken together, the increased thermal and acidic resistances of the recombinant H9N2-TM virus suggest that the substitution in the transmembrane (TM) domain can affect the stability of the HA, therefore alters viral biological characteristics. To explore whether the increased HA trimers and increased thermal and acidic resistances could result in better immune responses thus providing better protection in mice, we first vaccinated six-week-old mice with inactivated recombinant H9N2-WT and H9N2-TM viruses twice to check antibody responses.	./cache/cord-002802-594mmlk8.txt	./txt/cord-002802-594mmlk8.txt