id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-002373-rueg4gsj	Huang, Ao	Quantitative Fluorescence Quenching on Antibody-conjugated Graphene Oxide as a Platform for Protein Sensing	2017-01-13		.txt	text/plain	3674	203	54	Then, analyte IgG proteins in increasing concentrations were added to a set of buffer solutions containing antibody-conjugated GO. As the number density of the binding sites was limited on the modified GO, more adsorbed analyte IgG proteins resulted in a stronger fluorescence signal from free IgG-FITCs in the solutions. Therefore, there is a positive and quantitative correlation between the analyte IgG concentration and the fluorescence intensity of free IgG-FITCs. According to this principle, our assay relies on the effectiveness of GO as an energy acceptor to provide efficient fluorescence quenching. The experiment was repeated 4 times, and the average of the results was plotted in Fig. 4(a) , from which we can see the assay's fluorescence signal increased readily with the increasing analyte IgG concentration. We established an immunosensor platform based on quantitative fluorescence quenching between fluorescein-labelled antigens and antibody-conjugated GO nanosheets, a process controlled quantitatively by the concentration of analyte proteins.	./cache/cord-002373-rueg4gsj.txt	./txt/cord-002373-rueg4gsj.txt