id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-002312-jyk7f8hz	Branton, W. G.	Brain microbiota disruption within inflammatory demyelinating lesions in multiple sclerosis	2016-11-28		.txt	text/plain	4611	231	41	Massively parallel (deep) sequencing (RNAseq) of total RNA permitted analysis of all RNA sequences in MS (n = 6) and nonMS (n = 6) white matter samples, revealing that bacterial RNA (ribosomal and non-ribosomal) sequences were detected in all nonMS and MS brain specimens, including MS patients with relapsing-remitting disease (receiving disease modifying therapy) (RR-MS, n = 3) and progressive (untreated) MS (P-MS; n = 3) ( Fig. 2A) . The current study shows the presence of bacterial RNA and DNA sequences and proteins in human brain which are disrupted in conjunction with inflammatory demyelination in patients with MS. The present studies revealed the ratio of bacterium-encoded 16s rDNA to rRNA in matched brain samples to be ~1:2 in both white matter (and cortex, data not shown) with bacterial numbers of 1200-1400 genomes/cm 3 suggesting both bacterial burden and replication were low compared to active pathogenic infections in other tissues.	./cache/cord-002312-jyk7f8hz.txt	./txt/cord-002312-jyk7f8hz.txt