id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-002081-vi6rth9o	Zhang, Chao	Establishment and application of a real-time loop-mediated isothermal amplification system for the detection of CYP2C19 polymorphisms	2016-06-01		.txt	text/plain	3387	211	49	In this work, a rapid one-step SNP detection method, real-time loop-mediated isothermal amplification (RT-LAMP), was first applied for CYP2C19 polymorphisms testing. The optimized method was established with specifically designed primers for target amplification by real-time detection in approximately 30 min under isothermal conditions. The successful establishment of an inexpensive, rapid and real-time LAMP protocol for CYP2C19*2 and CYP2C19*3 detection is significant for the extension of this technique for genotyping other SNPs. Our results suggest applications for this RT-LAMP assay system for both basic research and clinical diagnosis in pharmacogenomics. In this study, the successful establishment of an inexpensive, rapid and real-time LAMP protocol for CYP2C19 SNP genotyping expanded the scope of application of this technique to human gene mutation detection. In summary, as a rapid, feasible and cost-efficient point-of-care (POC) SNP detection method, we demonstrated that RT-LAMP could quantitatively detect human genomic DNA with high specificity and sensitivity in a single step.	./cache/cord-002081-vi6rth9o.txt	./txt/cord-002081-vi6rth9o.txt