id	sid	tid	token	lemma	pos
cord-048471-7jszm1nd	1	1	key	key	JJ
cord-048471-7jszm1nd	1	2	:	:	:
cord-048471-7jszm1nd	1	3	cord-048471	cord-048471	NN
cord-048471-7jszm1nd	1	4	-	-	HYPH
cord-048471-7jszm1nd	1	5	7jszm1nd	7jszm1nd	CD
cord-048471-7jszm1nd	1	6	authors	author	NNS
cord-048471-7jszm1nd	1	7	:	:	:
cord-048471-7jszm1nd	1	8	Salim	Salim	NNP
cord-048471-7jszm1nd	1	9	,	,	,
cord-048471-7jszm1nd	1	10	Omar	Omar	NNP
cord-048471-7jszm1nd	1	11	;	;	:
cord-048471-7jszm1nd	1	12	Clarke	Clarke	NNP
cord-048471-7jszm1nd	1	13	,	,	,
cord-048471-7jszm1nd	1	14	Ian	Ian	NNP
cord-048471-7jszm1nd	1	15	N.	N.	NNP
cord-048471-7jszm1nd	1	16	;	;	:
cord-048471-7jszm1nd	1	17	Lambden	Lambden	NNP
cord-048471-7jszm1nd	1	18	,	,	,
cord-048471-7jszm1nd	1	19	Paul	Paul	NNP
cord-048471-7jszm1nd	1	20	R.	R.	NNP
cord-048471-7jszm1nd	1	21	title	title	NN
cord-048471-7jszm1nd	1	22	:	:	:
cord-048471-7jszm1nd	1	23	Functional	Functional	NNP
cord-048471-7jszm1nd	1	24	Analysis	Analysis	NNP
cord-048471-7jszm1nd	1	25	of	of	IN
cord-048471-7jszm1nd	1	26	the	the	DT
cord-048471-7jszm1nd	1	27	5′	5′	CD
cord-048471-7jszm1nd	1	28	Genomic	Genomic	NNP
cord-048471-7jszm1nd	1	29	Sequence	sequence	NN
cord-048471-7jszm1nd	1	30	of	of	IN
cord-048471-7jszm1nd	1	31	a	a	DT
cord-048471-7jszm1nd	1	32	Bovine	Bovine	NNP
cord-048471-7jszm1nd	1	33	Norovirus	Norovirus	NNP
cord-048471-7jszm1nd	1	34	date	date	NN
cord-048471-7jszm1nd	1	35	:	:	:
cord-048471-7jszm1nd	1	36	2008	2008	CD
cord-048471-7jszm1nd	1	37	-	-	SYM
cord-048471-7jszm1nd	1	38	05	05	CD
cord-048471-7jszm1nd	1	39	-	-	SYM
cord-048471-7jszm1nd	1	40	14	14	CD
cord-048471-7jszm1nd	1	41	journal	journal	NN
cord-048471-7jszm1nd	1	42	:	:	:
cord-048471-7jszm1nd	2	1	PLoS	PLoS	NNP
cord-048471-7jszm1nd	3	1	One	one	CD
cord-048471-7jszm1nd	3	2	DOI	doi	NN
cord-048471-7jszm1nd	3	3	:	:	:
cord-048471-7jszm1nd	3	4	10.1371	10.1371	CD
cord-048471-7jszm1nd	3	5	/	/	SYM
cord-048471-7jszm1nd	3	6	journal.pone.0002169	journal.pone.0002169	NN
cord-048471-7jszm1nd	3	7	sha	sha	NNP
cord-048471-7jszm1nd	3	8	:	:	:
cord-048471-7jszm1nd	3	9	702d0144bdeae0782bbebcbe41d432fa7ae41415	702d0144bdeae0782bbebcbe41d432fa7ae41415	CD
cord-048471-7jszm1nd	3	10	doc_id	doc_id	CD
cord-048471-7jszm1nd	3	11	:	:	:
cord-048471-7jszm1nd	3	12	48471	48471	CD
cord-048471-7jszm1nd	3	13	cord_uid	cord_uid	NNS
cord-048471-7jszm1nd	3	14	:	:	:
cord-048471-7jszm1nd	4	1	7jszm1nd	7jszm1nd	CD
cord-048471-7jszm1nd	5	1	BACKGROUND	background	NN
cord-048471-7jszm1nd	5	2	:	:	:
cord-048471-7jszm1nd	6	1	Jena	Jena	NNP
cord-048471-7jszm1nd	6	2	Virus	Virus	NNP
cord-048471-7jszm1nd	6	3	(	(	-LRB-
cord-048471-7jszm1nd	6	4	JV	JV	NNP
cord-048471-7jszm1nd	6	5	)	)	-RRB-
cord-048471-7jszm1nd	6	6	,	,	,
cord-048471-7jszm1nd	6	7	a	a	DT
cord-048471-7jszm1nd	6	8	bovine	bovine	JJ
cord-048471-7jszm1nd	6	9	Norovirus	norovirus	NN
cord-048471-7jszm1nd	6	10	,	,	,
cord-048471-7jszm1nd	6	11	causes	cause	VBZ
cord-048471-7jszm1nd	6	12	enteric	enteric	JJ
cord-048471-7jszm1nd	6	13	disease	disease	NN
cord-048471-7jszm1nd	6	14	in	in	IN
cord-048471-7jszm1nd	6	15	cattle	cattle	NNS
cord-048471-7jszm1nd	6	16	and	and	CC
cord-048471-7jszm1nd	6	17	represents	represent	VBZ
cord-048471-7jszm1nd	6	18	a	a	DT
cord-048471-7jszm1nd	6	19	potential	potential	JJ
cord-048471-7jszm1nd	6	20	model	model	NN
cord-048471-7jszm1nd	6	21	for	for	IN
cord-048471-7jszm1nd	6	22	the	the	DT
cord-048471-7jszm1nd	6	23	study	study	NN
cord-048471-7jszm1nd	6	24	of	of	IN
cord-048471-7jszm1nd	6	25	enteric	enteric	JJ
cord-048471-7jszm1nd	6	26	norovirus	norovirus	NN
cord-048471-7jszm1nd	6	27	infection	infection	NN
cord-048471-7jszm1nd	6	28	and	and	CC
cord-048471-7jszm1nd	6	29	pathogenesis	pathogenesis	NN
cord-048471-7jszm1nd	6	30	.	.	.
cord-048471-7jszm1nd	7	1	The	the	DT
cord-048471-7jszm1nd	7	2	positive	positive	JJ
cord-048471-7jszm1nd	7	3	sense	sense	NN
cord-048471-7jszm1nd	7	4	RNA	RNA	NNP
cord-048471-7jszm1nd	7	5	genome	genome	NN
cord-048471-7jszm1nd	7	6	of	of	IN
cord-048471-7jszm1nd	7	7	JV	JV	NNP
cord-048471-7jszm1nd	7	8	is	be	VBZ
cord-048471-7jszm1nd	7	9	organised	organise	VBN
cord-048471-7jszm1nd	7	10	into	into	IN
cord-048471-7jszm1nd	7	11	ORF1	ORF1	NNP
cord-048471-7jszm1nd	7	12	(	(	-LRB-
cord-048471-7jszm1nd	7	13	non	non	JJ
cord-048471-7jszm1nd	7	14	-	-	JJ
cord-048471-7jszm1nd	7	15	structural	structural	JJ
cord-048471-7jszm1nd	7	16	proteins	protein	NNS
cord-048471-7jszm1nd	7	17	)	)	-RRB-
cord-048471-7jszm1nd	7	18	,	,	,
cord-048471-7jszm1nd	7	19	ORF2	ORF2	NNP
cord-048471-7jszm1nd	7	20	(	(	-LRB-
cord-048471-7jszm1nd	7	21	major	major	JJ
cord-048471-7jszm1nd	7	22	capsid	capsid	NN
cord-048471-7jszm1nd	7	23	protein	protein	NN
cord-048471-7jszm1nd	7	24	)	)	-RRB-
cord-048471-7jszm1nd	7	25	and	and	CC
cord-048471-7jszm1nd	7	26	ORF3	ORF3	NNP
cord-048471-7jszm1nd	7	27	(	(	-LRB-
cord-048471-7jszm1nd	7	28	minor	minor	JJ
cord-048471-7jszm1nd	7	29	capsid	capsid	NN
cord-048471-7jszm1nd	7	30	protein	protein	NN
cord-048471-7jszm1nd	7	31	)	)	-RRB-
cord-048471-7jszm1nd	7	32	.	.	.
cord-048471-7jszm1nd	8	1	The	the	DT
cord-048471-7jszm1nd	8	2	lack	lack	NN
cord-048471-7jszm1nd	8	3	of	of	IN
cord-048471-7jszm1nd	8	4	a	a	DT
cord-048471-7jszm1nd	8	5	cell	cell	NN
cord-048471-7jszm1nd	8	6	culture	culture	NN
cord-048471-7jszm1nd	8	7	system	system	NN
cord-048471-7jszm1nd	8	8	for	for	IN
cord-048471-7jszm1nd	8	9	studying	study	VBG
cord-048471-7jszm1nd	8	10	JV	JV	NNP
cord-048471-7jszm1nd	8	11	replication	replication	NN
cord-048471-7jszm1nd	8	12	has	have	VBZ
cord-048471-7jszm1nd	8	13	meant	mean	VBN
cord-048471-7jszm1nd	8	14	that	that	IN
cord-048471-7jszm1nd	8	15	work	work	NN
cord-048471-7jszm1nd	8	16	to	to	IN
cord-048471-7jszm1nd	8	17	date	date	NN
cord-048471-7jszm1nd	8	18	has	have	VBZ
cord-048471-7jszm1nd	8	19	relied	rely	VBN
cord-048471-7jszm1nd	8	20	upon	upon	IN
cord-048471-7jszm1nd	8	21	in	in	FW
cord-048471-7jszm1nd	8	22	vitro	vitro	FW
cord-048471-7jszm1nd	8	23	systems	system	NNS
cord-048471-7jszm1nd	8	24	to	to	TO
cord-048471-7jszm1nd	8	25	study	study	VB
cord-048471-7jszm1nd	8	26	non	non	JJ
cord-048471-7jszm1nd	8	27	-	-	JJ
cord-048471-7jszm1nd	8	28	structural	structural	JJ
cord-048471-7jszm1nd	8	29	protein	protein	NN
cord-048471-7jszm1nd	8	30	synthesis	synthesis	NN
cord-048471-7jszm1nd	8	31	and	and	CC
cord-048471-7jszm1nd	8	32	processing	processing	NN
cord-048471-7jszm1nd	8	33	.	.	.
cord-048471-7jszm1nd	9	1	PRINCIPAL	principal	NN
cord-048471-7jszm1nd	9	2	FINDINGS	finding	NNS
cord-048471-7jszm1nd	9	3	:	:	:
cord-048471-7jszm1nd	10	1	Only	only	RB
cord-048471-7jszm1nd	10	2	two	two	CD
cord-048471-7jszm1nd	10	3	of	of	IN
cord-048471-7jszm1nd	10	4	the	the	DT
cord-048471-7jszm1nd	10	5	three	three	CD
cord-048471-7jszm1nd	10	6	major	major	JJ
cord-048471-7jszm1nd	10	7	ORF1	ORF1	NNP
cord-048471-7jszm1nd	10	8	proteins	protein	NNS
cord-048471-7jszm1nd	10	9	were	be	VBD
cord-048471-7jszm1nd	10	10	identified	identify	VBN
cord-048471-7jszm1nd	10	11	(	(	-LRB-
cord-048471-7jszm1nd	10	12	p110	p110	NNP
cord-048471-7jszm1nd	10	13	and	and	CC
cord-048471-7jszm1nd	10	14	2C	2C	NNP
cord-048471-7jszm1nd	10	15	)	)	-RRB-
cord-048471-7jszm1nd	10	16	following	follow	VBG
cord-048471-7jszm1nd	10	17	in	in	IN
cord-048471-7jszm1nd	10	18	vitro	vitro	FW
cord-048471-7jszm1nd	10	19	translation	translation	NN
cord-048471-7jszm1nd	10	20	of	of	IN
cord-048471-7jszm1nd	10	21	JV	JV	NNP
cord-048471-7jszm1nd	10	22	RNA	RNA	NNP
cord-048471-7jszm1nd	10	23	,	,	,
cord-048471-7jszm1nd	10	24	the	the	DT
cord-048471-7jszm1nd	10	25	N	n	CD
cord-048471-7jszm1nd	10	26	-	-	HYPH
cord-048471-7jszm1nd	10	27	term	term	NN
cord-048471-7jszm1nd	10	28	protein	protein	NN
cord-048471-7jszm1nd	10	29	was	be	VBD
cord-048471-7jszm1nd	10	30	not	not	RB
cord-048471-7jszm1nd	10	31	detected	detect	VBN
cord-048471-7jszm1nd	10	32	.	.	.
cord-048471-7jszm1nd	11	1	The	the	DT
cord-048471-7jszm1nd	11	2	N	n	CD
cord-048471-7jszm1nd	11	3	-	-	HYPH
cord-048471-7jszm1nd	11	4	term	term	NN
cord-048471-7jszm1nd	11	5	encoding	encode	VBG
cord-048471-7jszm1nd	11	6	genomic	genomic	JJ
cord-048471-7jszm1nd	11	7	sequence	sequence	NN
cord-048471-7jszm1nd	11	8	(	(	-LRB-
cord-048471-7jszm1nd	11	9	5′GS	5′gs	CD
cord-048471-7jszm1nd	11	10	)	)	-RRB-
cord-048471-7jszm1nd	11	11	was	be	VBD
cord-048471-7jszm1nd	11	12	tested	test	VBN
cord-048471-7jszm1nd	11	13	for	for	IN
cord-048471-7jszm1nd	11	14	IRES	IRES	NNP
cord-048471-7jszm1nd	11	15	-	-	HYPH
cord-048471-7jszm1nd	11	16	like	like	JJ
cord-048471-7jszm1nd	11	17	function	function	NN
cord-048471-7jszm1nd	11	18	in	in	IN
cord-048471-7jszm1nd	11	19	a	a	DT
cord-048471-7jszm1nd	11	20	bi	bi	JJ
cord-048471-7jszm1nd	11	21	-	-	JJ
cord-048471-7jszm1nd	11	22	cistronic	cistronic	JJ
cord-048471-7jszm1nd	11	23	system	system	NN
cord-048471-7jszm1nd	11	24	and	and	CC
cord-048471-7jszm1nd	11	25	displayed	display	VBD
cord-048471-7jszm1nd	11	26	no	no	DT
cord-048471-7jszm1nd	11	27	evidence	evidence	NN
cord-048471-7jszm1nd	11	28	of	of	IN
cord-048471-7jszm1nd	11	29	IRES	IRES	NNP
cord-048471-7jszm1nd	11	30	-	-	HYPH
cord-048471-7jszm1nd	11	31	like	like	JJ
cord-048471-7jszm1nd	11	32	activity	activity	NN
cord-048471-7jszm1nd	11	33	.	.	.
cord-048471-7jszm1nd	12	1	The	the	DT
cord-048471-7jszm1nd	12	2	site	site	NN
cord-048471-7jszm1nd	12	3	of	of	IN
cord-048471-7jszm1nd	12	4	translation	translation	NN
cord-048471-7jszm1nd	12	5	initiation	initiation	NN
cord-048471-7jszm1nd	12	6	in	in	IN
cord-048471-7jszm1nd	12	7	JV	JV	NNP
cord-048471-7jszm1nd	12	8	was	be	VBD
cord-048471-7jszm1nd	12	9	determined	determine	VBN
cord-048471-7jszm1nd	12	10	to	to	TO
cord-048471-7jszm1nd	12	11	be	be	VB
cord-048471-7jszm1nd	12	12	at	at	IN
cord-048471-7jszm1nd	12	13	the	the	DT
cord-048471-7jszm1nd	12	14	predicted	predict	VBN
cord-048471-7jszm1nd	12	15	nucleotide	nucleotide	JJ
cord-048471-7jszm1nd	12	16	22	22	CD
cord-048471-7jszm1nd	12	17	.	.	.
cord-048471-7jszm1nd	13	1	Following	follow	VBG
cord-048471-7jszm1nd	13	2	the	the	DT
cord-048471-7jszm1nd	13	3	insertion	insertion	NN
cord-048471-7jszm1nd	13	4	of	of	IN
cord-048471-7jszm1nd	13	5	an	an	DT
cord-048471-7jszm1nd	13	6	epitope	epitope	NN
cord-048471-7jszm1nd	13	7	within	within	IN
cord-048471-7jszm1nd	13	8	the	the	DT
cord-048471-7jszm1nd	13	9	5′GS	5′gs	CD
cord-048471-7jszm1nd	14	1	the	the	DT
cord-048471-7jszm1nd	14	2	JV	JV	NNP
cord-048471-7jszm1nd	14	3	N	N	NNP
cord-048471-7jszm1nd	14	4	-	-	HYPH
cord-048471-7jszm1nd	14	5	term	term	NN
cord-048471-7jszm1nd	14	6	protein	protein	NN
cord-048471-7jszm1nd	14	7	was	be	VBD
cord-048471-7jszm1nd	14	8	identified	identify	VBN
cord-048471-7jszm1nd	14	9	in	in	IN
cord-048471-7jszm1nd	14	10	vitro	vitro	FW
cord-048471-7jszm1nd	14	11	and	and	CC
cord-048471-7jszm1nd	14	12	within	within	IN
cord-048471-7jszm1nd	14	13	RNA	RNA	NNP
cord-048471-7jszm1nd	14	14	transfected	transfecte	VBN
cord-048471-7jszm1nd	14	15	cells	cell	NNS
cord-048471-7jszm1nd	14	16	.	.	.
cord-048471-7jszm1nd	15	1	CONCLUSIONS	conclusion	NNS
cord-048471-7jszm1nd	15	2	:	:	:
cord-048471-7jszm1nd	16	1	The	the	DT
cord-048471-7jszm1nd	16	2	in	in	IN
cord-048471-7jszm1nd	16	3	vitro	vitro	FW
cord-048471-7jszm1nd	16	4	transcription	transcription	NN
cord-048471-7jszm1nd	16	5	/	/	SYM
cord-048471-7jszm1nd	16	6	translation	translation	NN
cord-048471-7jszm1nd	16	7	system	system	NN
cord-048471-7jszm1nd	16	8	is	be	VBZ
cord-048471-7jszm1nd	16	9	currently	currently	RB
cord-048471-7jszm1nd	16	10	the	the	DT
cord-048471-7jszm1nd	16	11	best	good	JJS
cord-048471-7jszm1nd	16	12	system	system	NN
cord-048471-7jszm1nd	16	13	for	for	IN
cord-048471-7jszm1nd	16	14	analysing	analyse	VBG
cord-048471-7jszm1nd	16	15	protein	protein	NN
cord-048471-7jszm1nd	16	16	synthesis	synthesis	NN
cord-048471-7jszm1nd	16	17	and	and	CC
cord-048471-7jszm1nd	16	18	processing	processing	NN
cord-048471-7jszm1nd	16	19	in	in	IN
cord-048471-7jszm1nd	16	20	JV	JV	NNP
cord-048471-7jszm1nd	16	21	.	.	.
cord-048471-7jszm1nd	17	1	Unlike	unlike	IN
cord-048471-7jszm1nd	17	2	similarly	similarly	RB
cord-048471-7jszm1nd	17	3	studied	study	VBN
cord-048471-7jszm1nd	17	4	human	human	JJ
cord-048471-7jszm1nd	17	5	noroviruses	noroviruse	NNS
cord-048471-7jszm1nd	17	6	JV	JV	NNP
cord-048471-7jszm1nd	17	7	initially	initially	RB
cord-048471-7jszm1nd	17	8	did	do	VBD
cord-048471-7jszm1nd	17	9	not	not	RB
cord-048471-7jszm1nd	17	10	appear	appear	VB
cord-048471-7jszm1nd	17	11	to	to	TO
cord-048471-7jszm1nd	17	12	express	express	VB
cord-048471-7jszm1nd	17	13	the	the	DT
cord-048471-7jszm1nd	17	14	N	n	CD
cord-048471-7jszm1nd	17	15	-	-	HYPH
cord-048471-7jszm1nd	17	16	terminal	terminal	NN
cord-048471-7jszm1nd	17	17	protein	protein	NN
cord-048471-7jszm1nd	17	18	,	,	,
cord-048471-7jszm1nd	17	19	presenting	present	VBG
cord-048471-7jszm1nd	17	20	the	the	DT
cord-048471-7jszm1nd	17	21	possibility	possibility	NN
cord-048471-7jszm1nd	17	22	that	that	IN
cord-048471-7jszm1nd	17	23	the	the	DT
cord-048471-7jszm1nd	17	24	encoding	encoding	NN
cord-048471-7jszm1nd	17	25	RNA	RNA	NNP
cord-048471-7jszm1nd	17	26	sequence	sequence	NN
cord-048471-7jszm1nd	17	27	had	have	VBD
cord-048471-7jszm1nd	17	28	a	a	DT
cord-048471-7jszm1nd	17	29	regulatory	regulatory	JJ
cord-048471-7jszm1nd	17	30	function	function	NN
cord-048471-7jszm1nd	17	31	,	,	,
cord-048471-7jszm1nd	17	32	most	most	RBS
cord-048471-7jszm1nd	17	33	likely	likely	RB
cord-048471-7jszm1nd	17	34	involved	involve	VBN
cord-048471-7jszm1nd	17	35	in	in	IN
cord-048471-7jszm1nd	17	36	translation	translation	NN
cord-048471-7jszm1nd	17	37	initiation	initiation	NN
cord-048471-7jszm1nd	17	38	in	in	IN
cord-048471-7jszm1nd	17	39	an	an	DT
cord-048471-7jszm1nd	17	40	IRES	IRES	NNP
cord-048471-7jszm1nd	17	41	-	-	HYPH
cord-048471-7jszm1nd	17	42	like	like	JJ
cord-048471-7jszm1nd	17	43	manner	manner	NN
cord-048471-7jszm1nd	17	44	.	.	.
cord-048471-7jszm1nd	18	1	This	this	DT
cord-048471-7jszm1nd	18	2	was	be	VBD
cord-048471-7jszm1nd	18	3	not	not	RB
cord-048471-7jszm1nd	18	4	the	the	DT
cord-048471-7jszm1nd	18	5	case	case	NN
cord-048471-7jszm1nd	18	6	and	and	CC
cord-048471-7jszm1nd	18	7	,	,	,
cord-048471-7jszm1nd	18	8	following	follow	VBG
cord-048471-7jszm1nd	18	9	determination	determination	NN
cord-048471-7jszm1nd	18	10	of	of	IN
cord-048471-7jszm1nd	18	11	the	the	DT
cord-048471-7jszm1nd	18	12	site	site	NN
cord-048471-7jszm1nd	18	13	of	of	IN
cord-048471-7jszm1nd	18	14	translation	translation	NN
cord-048471-7jszm1nd	18	15	initiation	initiation	NN
cord-048471-7jszm1nd	19	1	the	the	DT
cord-048471-7jszm1nd	19	2	N	n	CD
cord-048471-7jszm1nd	19	3	-	-	HYPH
cord-048471-7jszm1nd	19	4	term	term	NN
cord-048471-7jszm1nd	19	5	protein	protein	NN
cord-048471-7jszm1nd	19	6	was	be	VBD
cord-048471-7jszm1nd	19	7	detected	detect	VBN
cord-048471-7jszm1nd	19	8	using	use	VBG
cord-048471-7jszm1nd	19	9	an	an	DT
cord-048471-7jszm1nd	19	10	epitope	epitope	NN
cord-048471-7jszm1nd	19	11	tag	tag	NN
cord-048471-7jszm1nd	19	12	,	,	,
cord-048471-7jszm1nd	19	13	both	both	CC
cord-048471-7jszm1nd	19	14	in	in	IN
cord-048471-7jszm1nd	19	15	vitro	vitro	FW
cord-048471-7jszm1nd	19	16	and	and	CC
cord-048471-7jszm1nd	19	17	in	in	IN
cord-048471-7jszm1nd	19	18	vivo	vivo	NNP
cord-048471-7jszm1nd	19	19	.	.	.
cord-048471-7jszm1nd	20	1	Although	although	IN
cord-048471-7jszm1nd	20	2	slightly	slightly	RB
cord-048471-7jszm1nd	20	3	larger	large	JJR
cord-048471-7jszm1nd	20	4	than	than	IN
cord-048471-7jszm1nd	20	5	predicted	predict	VBD
cord-048471-7jszm1nd	20	6	the	the	DT
cord-048471-7jszm1nd	20	7	N	n	CD
cord-048471-7jszm1nd	20	8	-	-	HYPH
cord-048471-7jszm1nd	20	9	term	term	NN
cord-048471-7jszm1nd	20	10	protein	protein	NN
cord-048471-7jszm1nd	20	11	was	be	VBD
cord-048471-7jszm1nd	20	12	detected	detect	VBN
cord-048471-7jszm1nd	20	13	in	in	IN
cord-048471-7jszm1nd	20	14	a	a	DT
cord-048471-7jszm1nd	20	15	processed	process	VBN
cord-048471-7jszm1nd	20	16	form	form	NN
cord-048471-7jszm1nd	20	17	in	in	IN
cord-048471-7jszm1nd	20	18	vivo	vivo	NN
cord-048471-7jszm1nd	20	19	,	,	,
cord-048471-7jszm1nd	20	20	thus	thus	RB
cord-048471-7jszm1nd	20	21	not	not	RB
cord-048471-7jszm1nd	20	22	only	only	RB
cord-048471-7jszm1nd	20	23	demonstrating	demonstrate	VBG
cord-048471-7jszm1nd	20	24	initial	initial	JJ
cord-048471-7jszm1nd	20	25	translation	translation	NN
cord-048471-7jszm1nd	20	26	of	of	IN
cord-048471-7jszm1nd	20	27	the	the	DT
cord-048471-7jszm1nd	20	28	ORF1	ORF1	NNP
cord-048471-7jszm1nd	20	29	polyprotein	polyprotein	NN
cord-048471-7jszm1nd	20	30	but	but	CC
cord-048471-7jszm1nd	20	31	also	also	RB
cord-048471-7jszm1nd	20	32	activity	activity	NN
cord-048471-7jszm1nd	20	33	of	of	IN
cord-048471-7jszm1nd	20	34	the	the	DT
cord-048471-7jszm1nd	20	35	viral	viral	JJ
cord-048471-7jszm1nd	20	36	protease	protease	NN
cord-048471-7jszm1nd	20	37	.	.	.
cord-048471-7jszm1nd	21	1	These	these	DT
cord-048471-7jszm1nd	21	2	findings	finding	NNS
cord-048471-7jszm1nd	21	3	indicate	indicate	VBP
cord-048471-7jszm1nd	21	4	that	that	IN
cord-048471-7jszm1nd	21	5	the	the	DT
cord-048471-7jszm1nd	21	6	block	block	NN
cord-048471-7jszm1nd	21	7	to	to	IN
cord-048471-7jszm1nd	21	8	noroviral	noroviral	NNP
cord-048471-7jszm1nd	21	9	replication	replication	NN
cord-048471-7jszm1nd	21	10	in	in	IN
cord-048471-7jszm1nd	21	11	cultured	cultured	JJ
cord-048471-7jszm1nd	21	12	cells	cell	NNS
cord-048471-7jszm1nd	21	13	lies	lie	VBZ
cord-048471-7jszm1nd	21	14	elsewhere	elsewhere	RB
cord-048471-7jszm1nd	21	15	.	.	.
cord-048471-7jszm1nd	22	1	Jena	Jena	NNP
cord-048471-7jszm1nd	22	2	virus	virus	NN
cord-048471-7jszm1nd	22	3	,	,	,
cord-048471-7jszm1nd	22	4	a	a	DT
cord-048471-7jszm1nd	22	5	bovine	bovine	JJ
cord-048471-7jszm1nd	22	6	norovirus	norovirus	NN
cord-048471-7jszm1nd	22	7	,	,	,
cord-048471-7jszm1nd	22	8	is	be	VBZ
cord-048471-7jszm1nd	22	9	a	a	DT
cord-048471-7jszm1nd	22	10	member	member	NN
cord-048471-7jszm1nd	22	11	of	of	IN
cord-048471-7jszm1nd	22	12	the	the	DT
cord-048471-7jszm1nd	22	13	Caliciviridae	Caliciviridae	NNP
cord-048471-7jszm1nd	22	14	family	family	NN
cord-048471-7jszm1nd	22	15	of	of	IN
cord-048471-7jszm1nd	22	16	positive	positive	JJ
cord-048471-7jszm1nd	22	17	sense	sense	NN
cord-048471-7jszm1nd	22	18	RNA	RNA	NNP
cord-048471-7jszm1nd	22	19	viruses	virus	NNS
cord-048471-7jszm1nd	22	20	and	and	CC
cord-048471-7jszm1nd	22	21	was	be	VBD
cord-048471-7jszm1nd	22	22	first	first	RB
cord-048471-7jszm1nd	22	23	isolated	isolate	VBN
cord-048471-7jszm1nd	22	24	from	from	IN
cord-048471-7jszm1nd	22	25	the	the	DT
cord-048471-7jszm1nd	22	26	diarrhoeic	diarrhoeic	JJ
cord-048471-7jszm1nd	22	27	stools	stool	NNS
cord-048471-7jszm1nd	22	28	of	of	IN
cord-048471-7jszm1nd	22	29	newborn	newborn	JJ
cord-048471-7jszm1nd	22	30	calves	calf	NNS
cord-048471-7jszm1nd	22	31	[	[	-LRB-
cord-048471-7jszm1nd	22	32	1	1	CD
cord-048471-7jszm1nd	22	33	,	,	,
cord-048471-7jszm1nd	22	34	2	2	CD
cord-048471-7jszm1nd	22	35	]	]	-RRB-
cord-048471-7jszm1nd	22	36	.	.	.
cord-048471-7jszm1nd	23	1	JV	JV	NNP
cord-048471-7jszm1nd	23	2	is	be	VBZ
cord-048471-7jszm1nd	23	3	a	a	DT
cord-048471-7jszm1nd	23	4	type	type	NN
cord-048471-7jszm1nd	23	5	I	-PRON-	PRP
cord-048471-7jszm1nd	23	6	genogroup	genogroup	NN
cord-048471-7jszm1nd	23	7	III	III	NNP
cord-048471-7jszm1nd	24	1	(	(	-LRB-
cord-048471-7jszm1nd	24	2	GIII	GIII	NNP
cord-048471-7jszm1nd	24	3	)	)	-RRB-
cord-048471-7jszm1nd	24	4	norovirus	norovirus	NN
cord-048471-7jszm1nd	24	5	which	which	WDT
cord-048471-7jszm1nd	24	6	is	be	VBZ
cord-048471-7jszm1nd	24	7	closely	closely	RB
cord-048471-7jszm1nd	24	8	related	related	JJ
cord-048471-7jszm1nd	24	9	to	to	IN
cord-048471-7jszm1nd	24	10	the	the	DT
cord-048471-7jszm1nd	24	11	type	type	NN
cord-048471-7jszm1nd	24	12	II	II	NNP
cord-048471-7jszm1nd	24	13	GIII	GIII	NNP
cord-048471-7jszm1nd	24	14	bovine	bovine	JJ
cord-048471-7jszm1nd	24	15	noroviruses	noroviruse	NNS
cord-048471-7jszm1nd	24	16	Newbury	Newbury	NNP
cord-048471-7jszm1nd	24	17	agent	agent	NN
cord-048471-7jszm1nd	24	18	2	2	CD
cord-048471-7jszm1nd	24	19	and	and	CC
cord-048471-7jszm1nd	24	20	Dumfries	Dumfries	NNP
cord-048471-7jszm1nd	24	21	[	[	-LRB-
cord-048471-7jszm1nd	24	22	3	3	CD
cord-048471-7jszm1nd	24	23	,	,	,
cord-048471-7jszm1nd	24	24	4	4	CD
cord-048471-7jszm1nd	24	25	]	]	-RRB-
cord-048471-7jszm1nd	24	26	.	.	.
cord-048471-7jszm1nd	25	1	The	the	DT
cord-048471-7jszm1nd	25	2	GIII	GIII	NNP
cord-048471-7jszm1nd	25	3	noroviruses	noroviruse	NNS
cord-048471-7jszm1nd	25	4	are	be	VBP
cord-048471-7jszm1nd	25	5	responsible	responsible	JJ
cord-048471-7jszm1nd	25	6	for	for	IN
cord-048471-7jszm1nd	25	7	causing	cause	VBG
cord-048471-7jszm1nd	25	8	enteric	enteric	JJ
cord-048471-7jszm1nd	25	9	disease	disease	NN
cord-048471-7jszm1nd	25	10	in	in	IN
cord-048471-7jszm1nd	25	11	cattle	cattle	NNS
cord-048471-7jszm1nd	25	12	[	[	-LRB-
cord-048471-7jszm1nd	25	13	2	2	CD
cord-048471-7jszm1nd	25	14	,	,	,
cord-048471-7jszm1nd	25	15	5	5	CD
cord-048471-7jszm1nd	25	16	]	]	-RRB-
cord-048471-7jszm1nd	25	17	and	and	CC
cord-048471-7jszm1nd	25	18	,	,	,
cord-048471-7jszm1nd	25	19	thus	thus	RB
cord-048471-7jszm1nd	25	20	,	,	,
cord-048471-7jszm1nd	25	21	likely	likely	RB
cord-048471-7jszm1nd	25	22	share	share	VBP
cord-048471-7jszm1nd	25	23	a	a	DT
cord-048471-7jszm1nd	25	24	similar	similar	JJ
cord-048471-7jszm1nd	25	25	tissue	tissue	NN
cord-048471-7jszm1nd	25	26	tropism	tropism	NN
cord-048471-7jszm1nd	25	27	to	to	IN
cord-048471-7jszm1nd	25	28	the	the	DT
cord-048471-7jszm1nd	25	29	human	human	NN
cord-048471-7jszm1nd	25	30	-	-	HYPH
cord-048471-7jszm1nd	25	31	associated	associate	VBN
cord-048471-7jszm1nd	25	32	enteric	enteric	JJ
cord-048471-7jszm1nd	25	33	noroviruses	noroviruse	NNS
cord-048471-7jszm1nd	25	34	.	.	.
cord-048471-7jszm1nd	26	1	Like	like	IN
cord-048471-7jszm1nd	26	2	human	human	JJ
cord-048471-7jszm1nd	26	3	noroviruses	noroviruse	NNS
cord-048471-7jszm1nd	26	4	[	[	-LRB-
cord-048471-7jszm1nd	26	5	6	6	CD
cord-048471-7jszm1nd	26	6	]	]	-RRB-
cord-048471-7jszm1nd	26	7	bovine	bovine	JJ
cord-048471-7jszm1nd	26	8	noroviruses	noroviruse	NNS
cord-048471-7jszm1nd	26	9	have	have	VBP
cord-048471-7jszm1nd	26	10	a	a	DT
cord-048471-7jszm1nd	26	11	high	high	JJ
cord-048471-7jszm1nd	26	12	seroprevalence	seroprevalence	NN
cord-048471-7jszm1nd	26	13	[	[	-LRB-
cord-048471-7jszm1nd	26	14	4	4	CD
cord-048471-7jszm1nd	26	15	]	]	-RRB-
cord-048471-7jszm1nd	26	16	.	.	.
cord-048471-7jszm1nd	27	1	JV	JV	NNP
cord-048471-7jszm1nd	27	2	is	be	VBZ
cord-048471-7jszm1nd	27	3	therefore	therefore	RB
cord-048471-7jszm1nd	27	4	a	a	DT
cord-048471-7jszm1nd	27	5	potentially	potentially	RB
cord-048471-7jszm1nd	27	6	useful	useful	JJ
cord-048471-7jszm1nd	27	7	model	model	NN
cord-048471-7jszm1nd	27	8	for	for	IN
cord-048471-7jszm1nd	27	9	studying	study	VBG
cord-048471-7jszm1nd	27	10	the	the	DT
cord-048471-7jszm1nd	27	11	molecular	molecular	JJ
cord-048471-7jszm1nd	27	12	biology	biology	NN
cord-048471-7jszm1nd	27	13	of	of	IN
cord-048471-7jszm1nd	27	14	enteric	enteric	JJ
cord-048471-7jszm1nd	27	15	norovirus	norovirus	NN
cord-048471-7jszm1nd	27	16	pathogenesis	pathogenesis	NN
cord-048471-7jszm1nd	27	17	and	and	CC
cord-048471-7jszm1nd	27	18	replication	replication	NN
cord-048471-7jszm1nd	27	19	.	.	.
cord-048471-7jszm1nd	28	1	The	the	DT
cord-048471-7jszm1nd	28	2	7.3	7.3	CD
cord-048471-7jszm1nd	28	3	kb	kb	NNP
cord-048471-7jszm1nd	28	4	polyadenylated	polyadenylate	VBD
cord-048471-7jszm1nd	28	5	RNA	RNA	NNP
cord-048471-7jszm1nd	28	6	genome	genome	NN
cord-048471-7jszm1nd	28	7	of	of	IN
cord-048471-7jszm1nd	28	8	JV	JV	NNP
cord-048471-7jszm1nd	28	9	has	have	VBZ
cord-048471-7jszm1nd	28	10	been	be	VBN
cord-048471-7jszm1nd	28	11	characterised	characterise	VBN
cord-048471-7jszm1nd	28	12	previously	previously	RB
cord-048471-7jszm1nd	28	13	[	[	-LRB-
cord-048471-7jszm1nd	28	14	7	7	CD
cord-048471-7jszm1nd	28	15	]	]	-RRB-
cord-048471-7jszm1nd	28	16	and	and	CC
cord-048471-7jszm1nd	28	17	,	,	,
cord-048471-7jszm1nd	28	18	like	like	IN
cord-048471-7jszm1nd	28	19	other	other	JJ
cord-048471-7jszm1nd	28	20	noroviruses	noroviruse	NNS
cord-048471-7jszm1nd	28	21	,	,	,
cord-048471-7jszm1nd	28	22	is	be	VBZ
cord-048471-7jszm1nd	28	23	organised	organise	VBN
cord-048471-7jszm1nd	28	24	into	into	IN
cord-048471-7jszm1nd	28	25	3	3	CD
cord-048471-7jszm1nd	28	26	open	open	JJ
cord-048471-7jszm1nd	28	27	reading	reading	NN
cord-048471-7jszm1nd	28	28	frames	frame	NNS
cord-048471-7jszm1nd	28	29	(	(	-LRB-
cord-048471-7jszm1nd	28	30	ORFs	ORFs	NNP
cord-048471-7jszm1nd	28	31	)	)	-RRB-
cord-048471-7jszm1nd	28	32	.	.	.
cord-048471-7jszm1nd	29	1	ORF1	ORF1	NNP
cord-048471-7jszm1nd	29	2	encodes	encode	VBZ
cord-048471-7jszm1nd	29	3	the	the	DT
cord-048471-7jszm1nd	29	4	non	non	JJ
cord-048471-7jszm1nd	29	5	-	-	JJ
cord-048471-7jszm1nd	29	6	structural	structural	JJ
cord-048471-7jszm1nd	29	7	proteins	protein	NNS
cord-048471-7jszm1nd	29	8	in	in	IN
cord-048471-7jszm1nd	29	9	the	the	DT
cord-048471-7jszm1nd	29	10	form	form	NN
cord-048471-7jszm1nd	29	11	of	of	IN
cord-048471-7jszm1nd	29	12	a	a	DT
cord-048471-7jszm1nd	29	13	large	large	JJ
cord-048471-7jszm1nd	29	14	185	185	CD
cord-048471-7jszm1nd	29	15	kDa	kDa	NNP
cord-048471-7jszm1nd	29	16	polyprotein	polyprotein	NNP
cord-048471-7jszm1nd	29	17	,	,	,
cord-048471-7jszm1nd	29	18	which	which	WDT
cord-048471-7jszm1nd	29	19	is	be	VBZ
cord-048471-7jszm1nd	29	20	subsequently	subsequently	RB
cord-048471-7jszm1nd	29	21	cleaved	cleave	VBN
cord-048471-7jszm1nd	29	22	into	into	IN
cord-048471-7jszm1nd	29	23	functional	functional	JJ
cord-048471-7jszm1nd	29	24	replication	replication	NN
cord-048471-7jszm1nd	29	25	proteins	protein	NNS
cord-048471-7jszm1nd	29	26	by	by	IN
cord-048471-7jszm1nd	29	27	the	the	DT
cord-048471-7jszm1nd	29	28	viral	viral	NN
cord-048471-7jszm1nd	29	29	encoded	encode	VBN
cord-048471-7jszm1nd	29	30	3C	3c	NN
cord-048471-7jszm1nd	29	31	-	-	HYPH
cord-048471-7jszm1nd	29	32	like	like	JJ
cord-048471-7jszm1nd	29	33	protease	protease	NN
cord-048471-7jszm1nd	29	34	.	.	.
cord-048471-7jszm1nd	30	1	ORF2	ORF2	NNP
cord-048471-7jszm1nd	30	2	encodes	encode	VBZ
cord-048471-7jszm1nd	30	3	the	the	DT
cord-048471-7jszm1nd	30	4	structural	structural	JJ
cord-048471-7jszm1nd	30	5	capsid	capsid	NN
cord-048471-7jszm1nd	30	6	protein	protein	NN
cord-048471-7jszm1nd	30	7	(	(	-LRB-
cord-048471-7jszm1nd	30	8	56	56	CD
cord-048471-7jszm1nd	30	9	kDa	kDa	NNPS
cord-048471-7jszm1nd	30	10	)	)	-RRB-
cord-048471-7jszm1nd	30	11	and	and	CC
cord-048471-7jszm1nd	30	12	ORF3	ORF3	NNP
cord-048471-7jszm1nd	30	13	encodes	encode	VBZ
cord-048471-7jszm1nd	30	14	a	a	DT
cord-048471-7jszm1nd	30	15	small	small	JJ
cord-048471-7jszm1nd	30	16	basic	basic	JJ
cord-048471-7jszm1nd	30	17	protein	protein	NN
cord-048471-7jszm1nd	30	18	,	,	,
cord-048471-7jszm1nd	30	19	which	which	WDT
cord-048471-7jszm1nd	30	20	has	have	VBZ
cord-048471-7jszm1nd	30	21	been	be	VBN
cord-048471-7jszm1nd	30	22	shown	show	VBN
cord-048471-7jszm1nd	30	23	to	to	TO
cord-048471-7jszm1nd	30	24	function	function	VB
cord-048471-7jszm1nd	30	25	as	as	IN
cord-048471-7jszm1nd	30	26	a	a	DT
cord-048471-7jszm1nd	30	27	minor	minor	JJ
cord-048471-7jszm1nd	30	28	capsid	capsid	NN
cord-048471-7jszm1nd	30	29	component	component	NN
cord-048471-7jszm1nd	30	30	[	[	-LRB-
cord-048471-7jszm1nd	30	31	8	8	CD
cord-048471-7jszm1nd	30	32	]	]	-RRB-
cord-048471-7jszm1nd	30	33	.	.	.
cord-048471-7jszm1nd	31	1	JV	JV	NNP
cord-048471-7jszm1nd	31	2	ORF1	ORF1	NNP
cord-048471-7jszm1nd	31	3	is	be	VBZ
cord-048471-7jszm1nd	31	4	consistent	consistent	JJ
cord-048471-7jszm1nd	31	5	with	with	IN
cord-048471-7jszm1nd	31	6	other	other	JJ
cord-048471-7jszm1nd	31	7	caliciviruses	caliciviruse	NNS
cord-048471-7jszm1nd	31	8	in	in	IN
cord-048471-7jszm1nd	31	9	that	that	IN
cord-048471-7jszm1nd	31	10	it	-PRON-	PRP
cord-048471-7jszm1nd	31	11	encodes	encode	VBZ
cord-048471-7jszm1nd	31	12	a	a	DT
cord-048471-7jszm1nd	31	13	39	39	CD
cord-048471-7jszm1nd	31	14	kDa	kDa	NNP
cord-048471-7jszm1nd	31	15	2C	2c	NN
cord-048471-7jszm1nd	31	16	-	-	HYPH
cord-048471-7jszm1nd	31	17	like	like	JJ
cord-048471-7jszm1nd	31	18	nucleoside	nucleoside	NN
cord-048471-7jszm1nd	31	19	triphosphatase	triphosphatase	NN
cord-048471-7jszm1nd	31	20	(	(	-LRB-
cord-048471-7jszm1nd	31	21	NTPase	NTPase	NNP
cord-048471-7jszm1nd	31	22	)	)	-RRB-
cord-048471-7jszm1nd	31	23	,	,	,
cord-048471-7jszm1nd	31	24	a	a	DT
cord-048471-7jszm1nd	31	25	3C	3c	NN
cord-048471-7jszm1nd	31	26	-	-	HYPH
cord-048471-7jszm1nd	31	27	like	like	JJ
cord-048471-7jszm1nd	31	28	protease	protease	NN
cord-048471-7jszm1nd	31	29	and	and	CC
cord-048471-7jszm1nd	31	30	a	a	DT
cord-048471-7jszm1nd	31	31	56	56	CD
cord-048471-7jszm1nd	31	32	kDa	kDa	NNP
cord-048471-7jszm1nd	31	33	3D	3D	NNP
cord-048471-7jszm1nd	31	34	-	-	HYPH
cord-048471-7jszm1nd	31	35	like	like	JJ
cord-048471-7jszm1nd	31	36	RNA	RNA	NNP
cord-048471-7jszm1nd	31	37	-	-	HYPH
cord-048471-7jszm1nd	31	38	dependent	dependent	JJ
cord-048471-7jszm1nd	31	39	RNA	RNA	NNP
cord-048471-7jszm1nd	31	40	polymerase	polymerase	NN
cord-048471-7jszm1nd	31	41	[	[	-LRB-
cord-048471-7jszm1nd	31	42	7	7	CD
cord-048471-7jszm1nd	31	43	,	,	,
cord-048471-7jszm1nd	31	44	[	[	-LRB-
cord-048471-7jszm1nd	31	45	9	9	CD
cord-048471-7jszm1nd	31	46	]	]	-RRB-
cord-048471-7jszm1nd	31	47	[	[	-LRB-
cord-048471-7jszm1nd	31	48	10	10	CD
cord-048471-7jszm1nd	31	49	]	]	-RRB-
cord-048471-7jszm1nd	31	50	[	[	-LRB-
cord-048471-7jszm1nd	31	51	11	11	CD
cord-048471-7jszm1nd	31	52	]	]	-RRB-
cord-048471-7jszm1nd	31	53	[	[	-LRB-
cord-048471-7jszm1nd	31	54	12	12	CD
cord-048471-7jszm1nd	31	55	]	]	-RRB-
cord-048471-7jszm1nd	31	56	[	[	-LRB-
cord-048471-7jszm1nd	31	57	13	13	CD
cord-048471-7jszm1nd	31	58	]	]	-RRB-
cord-048471-7jszm1nd	31	59	.	.	.
cord-048471-7jszm1nd	32	1	However	however	RB
cord-048471-7jszm1nd	32	2	,	,	,
cord-048471-7jszm1nd	32	3	the	the	DT
cord-048471-7jszm1nd	32	4	genomic	genomic	JJ
cord-048471-7jszm1nd	32	5	sequence	sequence	NN
cord-048471-7jszm1nd	32	6	within	within	IN
cord-048471-7jszm1nd	32	7	the	the	DT
cord-048471-7jszm1nd	32	8	59	59	CD
cord-048471-7jszm1nd	32	9	region	region	NN
cord-048471-7jszm1nd	32	10	of	of	IN
cord-048471-7jszm1nd	32	11	JV	JV	NNP
cord-048471-7jszm1nd	32	12	ORF1	ORF1	NNP
cord-048471-7jszm1nd	32	13	(	(	-LRB-
cord-048471-7jszm1nd	32	14	59GS	59gs	CD
cord-048471-7jszm1nd	32	15	)	)	-RRB-
cord-048471-7jszm1nd	32	16	displays	display	VBZ
cord-048471-7jszm1nd	32	17	a	a	DT
cord-048471-7jszm1nd	32	18	high	high	JJ
cord-048471-7jszm1nd	32	19	level	level	NN
cord-048471-7jszm1nd	32	20	of	of	IN
cord-048471-7jszm1nd	32	21	divergence	divergence	NN
cord-048471-7jszm1nd	32	22	.	.	.
cord-048471-7jszm1nd	33	1	This	this	DT
cord-048471-7jszm1nd	33	2	divergence	divergence	NN
cord-048471-7jszm1nd	33	3	is	be	VBZ
cord-048471-7jszm1nd	33	4	mainly	mainly	RB
cord-048471-7jszm1nd	33	5	attributed	attribute	VBN
cord-048471-7jszm1nd	33	6	to	to	IN
cord-048471-7jszm1nd	33	7	the	the	DT
cord-048471-7jszm1nd	33	8	presence	presence	NN
cord-048471-7jszm1nd	33	9	of	of	IN
cord-048471-7jszm1nd	33	10	several	several	JJ
cord-048471-7jszm1nd	33	11	proline	proline	NN
cord-048471-7jszm1nd	33	12	-	-	HYPH
cord-048471-7jszm1nd	33	13	encoding	encode	VBG
cord-048471-7jszm1nd	33	14	polypyrimidine	polypyrimidine	JJ
cord-048471-7jszm1nd	33	15	tracts	tract	NNS
cord-048471-7jszm1nd	33	16	within	within	IN
cord-048471-7jszm1nd	33	17	the	the	DT
cord-048471-7jszm1nd	33	18	region	region	NN
cord-048471-7jszm1nd	33	19	predicted	predict	VBD
cord-048471-7jszm1nd	33	20	to	to	TO
cord-048471-7jszm1nd	33	21	encode	encode	VB
cord-048471-7jszm1nd	33	22	a	a	DT
cord-048471-7jszm1nd	33	23	35	35	CD
cord-048471-7jszm1nd	33	24	kDa	kDa	NNP
cord-048471-7jszm1nd	33	25	Nterminal	Nterminal	NNP
cord-048471-7jszm1nd	33	26	protein	protein	NN
cord-048471-7jszm1nd	33	27	[	[	-LRB-
cord-048471-7jszm1nd	33	28	7	7	CD
cord-048471-7jszm1nd	33	29	]	]	-RRB-
cord-048471-7jszm1nd	33	30	.	.	.
cord-048471-7jszm1nd	34	1	The	the	DT
cord-048471-7jszm1nd	34	2	predicted	predict	VBN
cord-048471-7jszm1nd	34	3	size	size	NN
cord-048471-7jszm1nd	34	4	of	of	IN
cord-048471-7jszm1nd	34	5	N	n	CD
cord-048471-7jszm1nd	34	6	-	-	HYPH
cord-048471-7jszm1nd	34	7	terminal	terminal	NN
cord-048471-7jszm1nd	34	8	proteins	protein	NNS
cord-048471-7jszm1nd	34	9	relative	relative	JJ
cord-048471-7jszm1nd	34	10	to	to	IN
cord-048471-7jszm1nd	34	11	the	the	DT
cord-048471-7jszm1nd	34	12	size	size	NN
cord-048471-7jszm1nd	34	13	of	of	IN
cord-048471-7jszm1nd	34	14	the	the	DT
cord-048471-7jszm1nd	34	15	respective	respective	JJ
cord-048471-7jszm1nd	34	16	2C	2c	NN
cord-048471-7jszm1nd	34	17	proteins	protein	NNS
cord-048471-7jszm1nd	34	18	differs	differ	VBZ
cord-048471-7jszm1nd	34	19	within	within	IN
cord-048471-7jszm1nd	34	20	the	the	DT
cord-048471-7jszm1nd	34	21	norovirus	norovirus	NN
cord-048471-7jszm1nd	34	22	genus	genus	NN
cord-048471-7jszm1nd	34	23	.	.	.
cord-048471-7jszm1nd	35	1	Within	within	IN
cord-048471-7jszm1nd	35	2	the	the	DT
cord-048471-7jszm1nd	35	3	GI	GI	NNP
cord-048471-7jszm1nd	35	4	noroviruses	noroviruse	NNS
cord-048471-7jszm1nd	35	5	,	,	,
cord-048471-7jszm1nd	35	6	such	such	JJ
cord-048471-7jszm1nd	35	7	as	as	IN
cord-048471-7jszm1nd	35	8	Southampton	Southampton	NNP
cord-048471-7jszm1nd	35	9	virus	virus	NN
cord-048471-7jszm1nd	35	10	,	,	,
cord-048471-7jszm1nd	35	11	the	the	DT
cord-048471-7jszm1nd	35	12	N	n	CD
cord-048471-7jszm1nd	35	13	-	-	HYPH
cord-048471-7jszm1nd	35	14	terminal	terminal	NN
cord-048471-7jszm1nd	35	15	protein	protein	NN
cord-048471-7jszm1nd	35	16	(	(	-LRB-
cord-048471-7jszm1nd	35	17	44.8	44.8	CD
cord-048471-7jszm1nd	35	18	kDa	kDa	NNPS
cord-048471-7jszm1nd	35	19	)	)	-RRB-
cord-048471-7jszm1nd	35	20	is	be	VBZ
cord-048471-7jszm1nd	35	21	larger	large	JJR
cord-048471-7jszm1nd	35	22	in	in	IN
cord-048471-7jszm1nd	35	23	size	size	NN
cord-048471-7jszm1nd	35	24	compared	compare	VBN
cord-048471-7jszm1nd	35	25	to	to	IN
cord-048471-7jszm1nd	35	26	the	the	DT
cord-048471-7jszm1nd	35	27	2C	2C	NNP
cord-048471-7jszm1nd	35	28	protein	protein	NNP
cord-048471-7jszm1nd	35	29	(	(	-LRB-
cord-048471-7jszm1nd	35	30	39.6	39.6	CD
cord-048471-7jszm1nd	35	31	kDa	kDa	NNPS
cord-048471-7jszm1nd	35	32	)	)	-RRB-
cord-048471-7jszm1nd	35	33	.	.	.
cord-048471-7jszm1nd	36	1	This	this	DT
cord-048471-7jszm1nd	36	2	is	be	VBZ
cord-048471-7jszm1nd	36	3	in	in	IN
cord-048471-7jszm1nd	36	4	contrast	contrast	NN
cord-048471-7jszm1nd	36	5	to	to	IN
cord-048471-7jszm1nd	36	6	the	the	DT
cord-048471-7jszm1nd	36	7	GII	GII	NNP
cord-048471-7jszm1nd	36	8	noroviruses	noroviruse	NNS
cord-048471-7jszm1nd	36	9	,	,	,
cord-048471-7jszm1nd	36	10	such	such	JJ
cord-048471-7jszm1nd	36	11	as	as	IN
cord-048471-7jszm1nd	36	12	Lordsdale	Lordsdale	NNP
cord-048471-7jszm1nd	36	13	virus	virus	NN
cord-048471-7jszm1nd	36	14	and	and	CC
cord-048471-7jszm1nd	36	15	Camberwell	Camberwell	NNP
cord-048471-7jszm1nd	36	16	virus	virus	NN
cord-048471-7jszm1nd	36	17	,	,	,
cord-048471-7jszm1nd	36	18	in	in	IN
cord-048471-7jszm1nd	36	19	that	that	DT
cord-048471-7jszm1nd	36	20	the	the	DT
cord-048471-7jszm1nd	36	21	N	n	CD
cord-048471-7jszm1nd	36	22	-	-	HYPH
cord-048471-7jszm1nd	36	23	terminal	terminal	NN
cord-048471-7jszm1nd	36	24	protein	protein	NN
cord-048471-7jszm1nd	36	25	is	be	VBZ
cord-048471-7jszm1nd	36	26	smaller	small	JJR
cord-048471-7jszm1nd	36	27	in	in	IN
cord-048471-7jszm1nd	36	28	size	size	NN
cord-048471-7jszm1nd	36	29	compared	compare	VBN
cord-048471-7jszm1nd	36	30	to	to	IN
cord-048471-7jszm1nd	36	31	the	the	DT
cord-048471-7jszm1nd	36	32	2C	2C	NNP
cord-048471-7jszm1nd	36	33	protein	protein	NNP
cord-048471-7jszm1nd	36	34	[	[	-LRB-
cord-048471-7jszm1nd	36	35	11	11	CD
cord-048471-7jszm1nd	36	36	,	,	,
cord-048471-7jszm1nd	36	37	14	14	CD
cord-048471-7jszm1nd	36	38	]	]	-RRB-
cord-048471-7jszm1nd	36	39	.	.	.
cord-048471-7jszm1nd	37	1	This	this	DT
cord-048471-7jszm1nd	37	2	is	be	VBZ
cord-048471-7jszm1nd	37	3	also	also	RB
cord-048471-7jszm1nd	37	4	the	the	DT
cord-048471-7jszm1nd	37	5	case	case	NN
cord-048471-7jszm1nd	37	6	for	for	IN
cord-048471-7jszm1nd	37	7	Jena	Jena	NNP
cord-048471-7jszm1nd	37	8	virus	virus	NN
cord-048471-7jszm1nd	37	9	in	in	IN
cord-048471-7jszm1nd	37	10	which	which	WDT
cord-048471-7jszm1nd	37	11	the	the	DT
cord-048471-7jszm1nd	37	12	predicted	predict	VBN
cord-048471-7jszm1nd	37	13	JV	JV	NNP
cord-048471-7jszm1nd	37	14	N	N	NNP
cord-048471-7jszm1nd	37	15	-	-	HYPH
cord-048471-7jszm1nd	37	16	terminal	terminal	NN
cord-048471-7jszm1nd	37	17	protein	protein	NN
cord-048471-7jszm1nd	37	18	(	(	-LRB-
cord-048471-7jszm1nd	37	19	35	35	CD
cord-048471-7jszm1nd	37	20	kDa	kDa	NNS
cord-048471-7jszm1nd	37	21	)	)	-RRB-
cord-048471-7jszm1nd	37	22	is	be	VBZ
cord-048471-7jszm1nd	37	23	smaller	small	JJR
cord-048471-7jszm1nd	37	24	than	than	IN
cord-048471-7jszm1nd	37	25	the	the	DT
cord-048471-7jszm1nd	37	26	JV	JV	NNP
cord-048471-7jszm1nd	37	27	2C	2c	NN
cord-048471-7jszm1nd	37	28	protein	protein	NN
cord-048471-7jszm1nd	37	29	(	(	-LRB-
cord-048471-7jszm1nd	37	30	39	39	CD
cord-048471-7jszm1nd	37	31	kDa	kDa	NNS
cord-048471-7jszm1nd	37	32	)	)	-RRB-
cord-048471-7jszm1nd	37	33	[	[	-LRB-
cord-048471-7jszm1nd	37	34	7	7	CD
cord-048471-7jszm1nd	37	35	]	]	-RRB-
cord-048471-7jszm1nd	37	36	.	.	.
cord-048471-7jszm1nd	38	1	The	the	DT
cord-048471-7jszm1nd	38	2	norovirus	norovirus	NN
cord-048471-7jszm1nd	38	3	N	n	CD
cord-048471-7jszm1nd	38	4	-	-	HYPH
cord-048471-7jszm1nd	38	5	terminal	terminal	JJ
cord-048471-7jszm1nd	38	6	protein	protein	NN
cord-048471-7jszm1nd	38	7	varies	vary	VBZ
cord-048471-7jszm1nd	38	8	in	in	IN
cord-048471-7jszm1nd	38	9	relative	relative	JJ
cord-048471-7jszm1nd	38	10	size	size	NN
cord-048471-7jszm1nd	38	11	across	across	IN
cord-048471-7jszm1nd	38	12	the	the	DT
cord-048471-7jszm1nd	38	13	genus	genus	NN
cord-048471-7jszm1nd	38	14	,	,	,
cord-048471-7jszm1nd	38	15	and	and	CC
cord-048471-7jszm1nd	38	16	the	the	DT
cord-048471-7jszm1nd	38	17	encoding	encoding	NN
cord-048471-7jszm1nd	38	18	sequence	sequence	NN
cord-048471-7jszm1nd	38	19	bears	bear	VBZ
cord-048471-7jszm1nd	38	20	no	no	DT
cord-048471-7jszm1nd	38	21	similarity	similarity	NN
cord-048471-7jszm1nd	38	22	to	to	IN
cord-048471-7jszm1nd	38	23	other	other	JJ
cord-048471-7jszm1nd	38	24	cellular	cellular	JJ
cord-048471-7jszm1nd	38	25	or	or	CC
cord-048471-7jszm1nd	38	26	viral	viral	JJ
cord-048471-7jszm1nd	38	27	proteins	protein	NNS
cord-048471-7jszm1nd	38	28	.	.	.
cord-048471-7jszm1nd	39	1	Alignment	alignment	NN
cord-048471-7jszm1nd	39	2	of	of	IN
cord-048471-7jszm1nd	39	3	the	the	DT
cord-048471-7jszm1nd	39	4	N	n	CD
cord-048471-7jszm1nd	39	5	-	-	HYPH
cord-048471-7jszm1nd	39	6	term	term	NN
cord-048471-7jszm1nd	39	7	protein	protein	NN
cord-048471-7jszm1nd	39	8	sequences	sequence	NNS
cord-048471-7jszm1nd	39	9	of	of	IN
cord-048471-7jszm1nd	39	10	various	various	JJ
cord-048471-7jszm1nd	39	11	noroviruses	noroviruse	NNS
cord-048471-7jszm1nd	39	12	indicates	indicate	VBZ
cord-048471-7jszm1nd	39	13	little	little	JJ
cord-048471-7jszm1nd	39	14	similarity	similarity	NN
cord-048471-7jszm1nd	39	15	between	between	IN
cord-048471-7jszm1nd	39	16	genogroups	genogroup	NNS
cord-048471-7jszm1nd	39	17	within	within	IN
cord-048471-7jszm1nd	39	18	the	the	DT
cord-048471-7jszm1nd	39	19	first	first	JJ
cord-048471-7jszm1nd	39	20	180	180	CD
cord-048471-7jszm1nd	39	21	residues	residue	NNS
cord-048471-7jszm1nd	39	22	,	,	,
cord-048471-7jszm1nd	39	23	however	however	RB
cord-048471-7jszm1nd	39	24	towards	towards	IN
cord-048471-7jszm1nd	39	25	the	the	DT
cord-048471-7jszm1nd	39	26	Cterminal	Cterminal	NNP
cord-048471-7jszm1nd	39	27	end	end	NN
cord-048471-7jszm1nd	39	28	of	of	IN
cord-048471-7jszm1nd	39	29	the	the	DT
cord-048471-7jszm1nd	39	30	protein	protein	NN
cord-048471-7jszm1nd	39	31	similarity	similarity	NN
cord-048471-7jszm1nd	39	32	between	between	IN
cord-048471-7jszm1nd	39	33	the	the	DT
cord-048471-7jszm1nd	39	34	amino	amino	NN
cord-048471-7jszm1nd	39	35	acid	acid	NN
cord-048471-7jszm1nd	39	36	residues	residue	NNS
cord-048471-7jszm1nd	39	37	increases	increase	NNS
cord-048471-7jszm1nd	39	38	.	.	.
cord-048471-7jszm1nd	40	1	Recent	recent	JJ
cord-048471-7jszm1nd	40	2	studies	study	NNS
cord-048471-7jszm1nd	40	3	investigating	investigate	VBG
cord-048471-7jszm1nd	40	4	the	the	DT
cord-048471-7jszm1nd	40	5	functions	function	NNS
cord-048471-7jszm1nd	40	6	of	of	IN
cord-048471-7jszm1nd	40	7	the	the	DT
cord-048471-7jszm1nd	40	8	Norwalk	Norwalk	NNP
cord-048471-7jszm1nd	40	9	virus	virus	NN
cord-048471-7jszm1nd	40	10	N	n	CD
cord-048471-7jszm1nd	40	11	-	-	HYPH
cord-048471-7jszm1nd	40	12	terminal	terminal	NN
cord-048471-7jszm1nd	40	13	protein	protein	NN
cord-048471-7jszm1nd	40	14	have	have	VBP
cord-048471-7jszm1nd	40	15	successfully	successfully	RB
cord-048471-7jszm1nd	40	16	demonstrated	demonstrate	VBN
cord-048471-7jszm1nd	40	17	association	association	NN
cord-048471-7jszm1nd	40	18	with	with	IN
cord-048471-7jszm1nd	40	19	the	the	DT
cord-048471-7jszm1nd	40	20	Golgi	Golgi	NNP
cord-048471-7jszm1nd	40	21	apparatus	apparatus	NN
cord-048471-7jszm1nd	40	22	in	in	IN
cord-048471-7jszm1nd	40	23	transfected	transfecte	VBN
cord-048471-7jszm1nd	40	24	cells	cell	NNS
cord-048471-7jszm1nd	40	25	[	[	-LRB-
cord-048471-7jszm1nd	40	26	15	15	CD
cord-048471-7jszm1nd	40	27	]	]	-RRB-
cord-048471-7jszm1nd	40	28	.	.	.
cord-048471-7jszm1nd	41	1	In	in	IN
cord-048471-7jszm1nd	41	2	addition	addition	NN
cord-048471-7jszm1nd	41	3	this	this	DT
cord-048471-7jszm1nd	41	4	study	study	NN
cord-048471-7jszm1nd	41	5	also	also	RB
cord-048471-7jszm1nd	41	6	identified	identify	VBD
cord-048471-7jszm1nd	41	7	a	a	DT
cord-048471-7jszm1nd	41	8	picornaviral	picornaviral	JJ
cord-048471-7jszm1nd	41	9	2B	2B	NNP
cord-048471-7jszm1nd	41	10	like	like	IN
cord-048471-7jszm1nd	41	11	region	region	NN
cord-048471-7jszm1nd	41	12	within	within	IN
cord-048471-7jszm1nd	41	13	the	the	DT
cord-048471-7jszm1nd	41	14	N	n	CD
cord-048471-7jszm1nd	41	15	-	-	HYPH
cord-048471-7jszm1nd	41	16	terminal	terminal	NN
cord-048471-7jszm1nd	41	17	protein	protein	NN
cord-048471-7jszm1nd	41	18	,	,	,
cord-048471-7jszm1nd	41	19	suggesting	suggest	VBG
cord-048471-7jszm1nd	41	20	that	that	IN
cord-048471-7jszm1nd	41	21	the	the	DT
cord-048471-7jszm1nd	41	22	protein	protein	NN
cord-048471-7jszm1nd	41	23	is	be	VBZ
cord-048471-7jszm1nd	41	24	involved	involve	VBN
cord-048471-7jszm1nd	41	25	with	with	IN
cord-048471-7jszm1nd	41	26	host	host	NN
cord-048471-7jszm1nd	41	27	cell	cell	NN
cord-048471-7jszm1nd	41	28	membrane	membrane	NN
cord-048471-7jszm1nd	41	29	interactions	interaction	NNS
cord-048471-7jszm1nd	41	30	,	,	,
cord-048471-7jszm1nd	41	31	reinforcing	reinforce	VBG
cord-048471-7jszm1nd	41	32	other	other	JJ
cord-048471-7jszm1nd	41	33	findings	finding	NNS
cord-048471-7jszm1nd	41	34	that	that	WDT
cord-048471-7jszm1nd	41	35	have	have	VBP
cord-048471-7jszm1nd	41	36	suggested	suggest	VBN
cord-048471-7jszm1nd	41	37	that	that	IN
cord-048471-7jszm1nd	41	38	the	the	DT
cord-048471-7jszm1nd	41	39	Norwalk	Norwalk	NNP
cord-048471-7jszm1nd	41	40	virus	virus	NN
cord-048471-7jszm1nd	41	41	N	n	CD
cord-048471-7jszm1nd	41	42	-	-	HYPH
cord-048471-7jszm1nd	41	43	terminal	terminal	NN
cord-048471-7jszm1nd	41	44	protein	protein	NN
cord-048471-7jszm1nd	41	45	disrupts	disrupt	VBZ
cord-048471-7jszm1nd	41	46	intracellular	intracellular	JJ
cord-048471-7jszm1nd	41	47	protein	protein	NN
cord-048471-7jszm1nd	41	48	trafficking	trafficking	NN
cord-048471-7jszm1nd	41	49	,	,	,
cord-048471-7jszm1nd	41	50	including	include	VBG
cord-048471-7jszm1nd	41	51	proteins	protein	NNS
cord-048471-7jszm1nd	41	52	destined	destine	VBN
cord-048471-7jszm1nd	41	53	for	for	IN
cord-048471-7jszm1nd	41	54	the	the	DT
cord-048471-7jszm1nd	41	55	host	host	NN
cord-048471-7jszm1nd	41	56	cell	cell	NN
cord-048471-7jszm1nd	41	57	membrane	membrane	NN
cord-048471-7jszm1nd	41	58	[	[	-LRB-
cord-048471-7jszm1nd	41	59	16	16	CD
cord-048471-7jszm1nd	41	60	]	]	-RRB-
cord-048471-7jszm1nd	41	61	.	.	.
cord-048471-7jszm1nd	42	1	A	a	DT
cord-048471-7jszm1nd	42	2	3C	3c	NN
cord-048471-7jszm1nd	42	3	protease	protease	NN
cord-048471-7jszm1nd	42	4	-	-	HYPH
cord-048471-7jszm1nd	42	5	mediated	mediate	VBN
cord-048471-7jszm1nd	42	6	cleavage	cleavage	NN
cord-048471-7jszm1nd	42	7	event	event	NN
cord-048471-7jszm1nd	42	8	within	within	IN
cord-048471-7jszm1nd	42	9	the	the	DT
cord-048471-7jszm1nd	42	10	N	n	CD
cord-048471-7jszm1nd	42	11	-	-	HYPH
cord-048471-7jszm1nd	42	12	terminal	terminal	NN
cord-048471-7jszm1nd	42	13	protein	protein	NN
cord-048471-7jszm1nd	42	14	(	(	-LRB-
cord-048471-7jszm1nd	42	15	37	37	CD
cord-048471-7jszm1nd	42	16	kDa	kDa	NNS
cord-048471-7jszm1nd	42	17	)	)	-RRB-
cord-048471-7jszm1nd	42	18	was	be	VBD
cord-048471-7jszm1nd	42	19	described	describe	VBN
cord-048471-7jszm1nd	42	20	for	for	IN
cord-048471-7jszm1nd	42	21	Camberwell	Camberwell	NNP
cord-048471-7jszm1nd	42	22	virus	virus	NN
cord-048471-7jszm1nd	42	23	,	,	,
cord-048471-7jszm1nd	42	24	a	a	DT
cord-048471-7jszm1nd	42	25	genogroup	genogroup	NN
cord-048471-7jszm1nd	42	26	2	2	CD
cord-048471-7jszm1nd	42	27	norovirus	norovirus	NN
cord-048471-7jszm1nd	42	28	,	,	,
cord-048471-7jszm1nd	42	29	yielding	yield	VBG
cord-048471-7jszm1nd	42	30	proteins	protein	NNS
cord-048471-7jszm1nd	42	31	of	of	IN
cord-048471-7jszm1nd	42	32	22	22	CD
cord-048471-7jszm1nd	42	33	kDa	kDa	NNS
cord-048471-7jszm1nd	42	34	and	and	CC
cord-048471-7jszm1nd	42	35	15	15	CD
cord-048471-7jszm1nd	42	36	kDa	kDa	NNPS
cord-048471-7jszm1nd	42	37	[	[	-LRB-
cord-048471-7jszm1nd	42	38	17	17	CD
cord-048471-7jszm1nd	42	39	]	]	-RRB-
cord-048471-7jszm1nd	42	40	.	.	.
cord-048471-7jszm1nd	43	1	Based	base	VBN
cord-048471-7jszm1nd	43	2	on	on	IN
cord-048471-7jszm1nd	43	3	these	these	DT
cord-048471-7jszm1nd	43	4	observations	observation	NNS
cord-048471-7jszm1nd	43	5	and	and	CC
cord-048471-7jszm1nd	43	6	location	location	NN
cord-048471-7jszm1nd	43	7	within	within	IN
cord-048471-7jszm1nd	43	8	the	the	DT
cord-048471-7jszm1nd	43	9	genome	genome	NN
cord-048471-7jszm1nd	43	10	it	-PRON-	PRP
cord-048471-7jszm1nd	43	11	was	be	VBD
cord-048471-7jszm1nd	43	12	hypothesised	hypothesise	VBN
cord-048471-7jszm1nd	43	13	that	that	IN
cord-048471-7jszm1nd	43	14	the	the	DT
cord-048471-7jszm1nd	43	15	Nterminal	Nterminal	NNP
cord-048471-7jszm1nd	43	16	protein	protein	NN
cord-048471-7jszm1nd	43	17	of	of	IN
cord-048471-7jszm1nd	43	18	noroviruses	noroviruses	NNP
cord-048471-7jszm1nd	43	19	corresponds	correspond	VBZ
cord-048471-7jszm1nd	43	20	to	to	IN
cord-048471-7jszm1nd	43	21	the	the	DT
cord-048471-7jszm1nd	43	22	2AB	2ab	CD
cord-048471-7jszm1nd	43	23	region	region	NN
cord-048471-7jszm1nd	43	24	in	in	IN
cord-048471-7jszm1nd	43	25	picornaviruses	picornavirus	NNS
cord-048471-7jszm1nd	43	26	.	.	.
cord-048471-7jszm1nd	44	1	Another	another	DT
cord-048471-7jszm1nd	44	2	possibility	possibility	NN
cord-048471-7jszm1nd	44	3	is	be	VBZ
cord-048471-7jszm1nd	44	4	that	that	IN
cord-048471-7jszm1nd	44	5	the	the	DT
cord-048471-7jszm1nd	44	6	N	n	CD
cord-048471-7jszm1nd	44	7	-	-	HYPH
cord-048471-7jszm1nd	44	8	term	term	NN
cord-048471-7jszm1nd	44	9	encoding	encode	VBG
cord-048471-7jszm1nd	44	10	RNA	RNA	NNP
cord-048471-7jszm1nd	44	11	itself	-PRON-	PRP
cord-048471-7jszm1nd	44	12	serves	serve	VBZ
cord-048471-7jszm1nd	44	13	to	to	TO
cord-048471-7jszm1nd	44	14	function	function	VB
cord-048471-7jszm1nd	44	15	as	as	IN
cord-048471-7jszm1nd	44	16	a	a	DT
cord-048471-7jszm1nd	44	17	translational	translational	JJ
cord-048471-7jszm1nd	44	18	enhancer	enhancer	NN
cord-048471-7jszm1nd	44	19	by	by	IN
cord-048471-7jszm1nd	44	20	interacting	interact	VBG
cord-048471-7jszm1nd	44	21	with	with	IN
cord-048471-7jszm1nd	44	22	cellular	cellular	JJ
cord-048471-7jszm1nd	44	23	proteins	protein	NNS
cord-048471-7jszm1nd	44	24	involved	involve	VBN
cord-048471-7jszm1nd	44	25	in	in	IN
cord-048471-7jszm1nd	44	26	translation	translation	NN
cord-048471-7jszm1nd	44	27	.	.	.
cord-048471-7jszm1nd	45	1	Indeed	indeed	RB
cord-048471-7jszm1nd	45	2	,	,	,
cord-048471-7jszm1nd	45	3	this	this	DT
cord-048471-7jszm1nd	45	4	phenomenon	phenomenon	NN
cord-048471-7jszm1nd	45	5	has	have	VBZ
cord-048471-7jszm1nd	45	6	been	be	VBN
cord-048471-7jszm1nd	45	7	previously	previously	RB
cord-048471-7jszm1nd	45	8	reported	report	VBN
cord-048471-7jszm1nd	45	9	for	for	IN
cord-048471-7jszm1nd	45	10	Norwalk	Norwalk	NNP
cord-048471-7jszm1nd	45	11	virus	virus	NN
cord-048471-7jszm1nd	45	12	,	,	,
cord-048471-7jszm1nd	45	13	within	within	IN
cord-048471-7jszm1nd	45	14	which	which	WDT
cord-048471-7jszm1nd	45	15	a	a	DT
cord-048471-7jszm1nd	45	16	double	double	JJ
cord-048471-7jszm1nd	45	17	stem	stem	NN
cord-048471-7jszm1nd	45	18	loop	loop	NN
cord-048471-7jszm1nd	45	19	structure	structure	NN
cord-048471-7jszm1nd	45	20	has	have	VBZ
cord-048471-7jszm1nd	45	21	been	be	VBN
cord-048471-7jszm1nd	45	22	predicted	predict	VBN
cord-048471-7jszm1nd	45	23	at	at	IN
cord-048471-7jszm1nd	45	24	the	the	DT
cord-048471-7jszm1nd	45	25	59	59	CD
cord-048471-7jszm1nd	45	26	end	end	NN
cord-048471-7jszm1nd	45	27	of	of	IN
cord-048471-7jszm1nd	45	28	the	the	DT
cord-048471-7jszm1nd	45	29	genomic	genomic	JJ
cord-048471-7jszm1nd	45	30	RNA	RNA	NNP
cord-048471-7jszm1nd	45	31	[	[	-LRB-
cord-048471-7jszm1nd	45	32	18	18	CD
cord-048471-7jszm1nd	45	33	]	]	-RRB-
cord-048471-7jszm1nd	45	34	.	.	.
cord-048471-7jszm1nd	46	1	It	-PRON-	PRP
cord-048471-7jszm1nd	46	2	was	be	VBD
cord-048471-7jszm1nd	46	3	subsequently	subsequently	RB
cord-048471-7jszm1nd	46	4	demonstrated	demonstrate	VBN
cord-048471-7jszm1nd	46	5	that	that	IN
cord-048471-7jszm1nd	46	6	elements	element	NNS
cord-048471-7jszm1nd	46	7	within	within	IN
cord-048471-7jszm1nd	46	8	the	the	DT
cord-048471-7jszm1nd	46	9	59	59	CD
cord-048471-7jszm1nd	46	10	end	end	NN
cord-048471-7jszm1nd	46	11	of	of	IN
cord-048471-7jszm1nd	46	12	Norwalk	Norwalk	NNP
cord-048471-7jszm1nd	46	13	virus	virus	NN
cord-048471-7jszm1nd	46	14	bind	bind	VBP
cord-048471-7jszm1nd	46	15	specifically	specifically	RB
cord-048471-7jszm1nd	46	16	with	with	IN
cord-048471-7jszm1nd	46	17	cellular	cellular	JJ
cord-048471-7jszm1nd	46	18	proteins	protein	NNS
cord-048471-7jszm1nd	46	19	such	such	JJ
cord-048471-7jszm1nd	46	20	as	as	IN
cord-048471-7jszm1nd	46	21	La	La	NNP
cord-048471-7jszm1nd	46	22	,	,	,
cord-048471-7jszm1nd	46	23	PTB	PTB	NNP
cord-048471-7jszm1nd	46	24	and	and	CC
cord-048471-7jszm1nd	46	25	PCBP2	PCBP2	NNP
cord-048471-7jszm1nd	46	26	[	[	-LRB-
cord-048471-7jszm1nd	46	27	19	19	CD
cord-048471-7jszm1nd	46	28	]	]	-RRB-
cord-048471-7jszm1nd	46	29	which	which	WDT
cord-048471-7jszm1nd	46	30	have	have	VBP
cord-048471-7jszm1nd	46	31	all	all	DT
cord-048471-7jszm1nd	46	32	been	be	VBN
cord-048471-7jszm1nd	46	33	implicated	implicate	VBN
cord-048471-7jszm1nd	46	34	in	in	IN
cord-048471-7jszm1nd	46	35	IRES	IRES	NNP
cord-048471-7jszm1nd	46	36	-	-	HYPH
cord-048471-7jszm1nd	46	37	mediated	mediate	VBN
cord-048471-7jszm1nd	46	38	cap	cap	NN
cord-048471-7jszm1nd	46	39	-	-	HYPH
cord-048471-7jszm1nd	46	40	independent	independent	JJ
cord-048471-7jszm1nd	46	41	translation	translation	NN
cord-048471-7jszm1nd	46	42	in	in	IN
cord-048471-7jszm1nd	46	43	the	the	DT
cord-048471-7jszm1nd	46	44	closely	closely	RB
cord-048471-7jszm1nd	46	45	related	relate	VBN
cord-048471-7jszm1nd	46	46	picornaviruses	picornavirus	NNS
cord-048471-7jszm1nd	46	47	[	[	-LRB-
cord-048471-7jszm1nd	46	48	20	20	CD
cord-048471-7jszm1nd	46	49	]	]	-RRB-
cord-048471-7jszm1nd	46	50	[	[	-LRB-
cord-048471-7jszm1nd	46	51	21	21	CD
cord-048471-7jszm1nd	46	52	]	]	-RRB-
cord-048471-7jszm1nd	47	1	[	[	-LRB-
cord-048471-7jszm1nd	47	2	22	22	CD
cord-048471-7jszm1nd	47	3	]	]	-RRB-
cord-048471-7jszm1nd	47	4	[	[	-LRB-
cord-048471-7jszm1nd	47	5	23	23	CD
cord-048471-7jszm1nd	47	6	]	]	-RRB-
cord-048471-7jszm1nd	47	7	.	.	.
cord-048471-7jszm1nd	48	1	In	in	IN
cord-048471-7jszm1nd	48	2	this	this	DT
cord-048471-7jszm1nd	48	3	study	study	NN
cord-048471-7jszm1nd	48	4	the	the	DT
cord-048471-7jszm1nd	48	5	role	role	NN
cord-048471-7jszm1nd	48	6	of	of	IN
cord-048471-7jszm1nd	48	7	the	the	DT
cord-048471-7jszm1nd	48	8	JV	JV	NNP
cord-048471-7jszm1nd	48	9	59GS	59gs	CD
cord-048471-7jszm1nd	48	10	was	be	VBD
cord-048471-7jszm1nd	48	11	investigated	investigate	VBN
cord-048471-7jszm1nd	48	12	,	,	,
cord-048471-7jszm1nd	48	13	including	include	VBG
cord-048471-7jszm1nd	48	14	its	-PRON-	PRP$
cord-048471-7jszm1nd	48	15	potential	potential	NN
cord-048471-7jszm1nd	48	16	to	to	IN
cord-048471-7jszm1nd	48	17	direct	direct	JJ
cord-048471-7jszm1nd	48	18	capindependent	capindependent	JJ
cord-048471-7jszm1nd	48	19	translation	translation	NN
cord-048471-7jszm1nd	48	20	initiation	initiation	NN
cord-048471-7jszm1nd	48	21	.	.	.
cord-048471-7jszm1nd	49	1	The	the	DT
cord-048471-7jszm1nd	49	2	precise	precise	JJ
cord-048471-7jszm1nd	49	3	location	location	NN
cord-048471-7jszm1nd	49	4	of	of	IN
cord-048471-7jszm1nd	49	5	translation	translation	NN
cord-048471-7jszm1nd	49	6	initiation	initiation	NN
cord-048471-7jszm1nd	49	7	in	in	IN
cord-048471-7jszm1nd	49	8	JV	JV	NNP
cord-048471-7jszm1nd	49	9	was	be	VBD
cord-048471-7jszm1nd	49	10	also	also	RB
cord-048471-7jszm1nd	49	11	investigated	investigate	VBN
cord-048471-7jszm1nd	49	12	.	.	.
cord-048471-7jszm1nd	50	1	Previous	previous	JJ
cord-048471-7jszm1nd	50	2	studies	study	NNS
cord-048471-7jszm1nd	50	3	of	of	IN
cord-048471-7jszm1nd	50	4	norovirus	norovirus	NNP
cord-048471-7jszm1nd	50	5	polyprotein	polyprotein	NNP
cord-048471-7jszm1nd	50	6	processing	processing	NN
cord-048471-7jszm1nd	50	7	have	have	VBP
cord-048471-7jszm1nd	50	8	yielded	yield	VBN
cord-048471-7jszm1nd	50	9	three	three	CD
cord-048471-7jszm1nd	50	10	major	major	JJ
cord-048471-7jszm1nd	50	11	products	product	NNS
cord-048471-7jszm1nd	50	12	following	follow	VBG
cord-048471-7jszm1nd	50	13	in	in	IN
cord-048471-7jszm1nd	50	14	vitro	vitro	FW
cord-048471-7jszm1nd	50	15	transcription	transcription	NN
cord-048471-7jszm1nd	50	16	and	and	CC
cord-048471-7jszm1nd	50	17	translation	translation	NN
cord-048471-7jszm1nd	50	18	,	,	,
cord-048471-7jszm1nd	50	19	representing	represent	VBG
cord-048471-7jszm1nd	50	20	the	the	DT
cord-048471-7jszm1nd	50	21	uncleaved	uncleaved	JJ
cord-048471-7jszm1nd	50	22	3ABCD	3ABCD	NNP
cord-048471-7jszm1nd	50	23	,	,	,
cord-048471-7jszm1nd	50	24	N	n	CD
cord-048471-7jszm1nd	50	25	-	-	HYPH
cord-048471-7jszm1nd	50	26	term	term	NN
cord-048471-7jszm1nd	50	27	and	and	CC
cord-048471-7jszm1nd	50	28	2C	2c	NN
cord-048471-7jszm1nd	50	29	proteins	protein	NNS
cord-048471-7jszm1nd	50	30	.	.	.
cord-048471-7jszm1nd	51	1	However	however	RB
cord-048471-7jszm1nd	51	2	,	,	,
cord-048471-7jszm1nd	51	3	initial	initial	JJ
cord-048471-7jszm1nd	51	4	analysis	analysis	NN
cord-048471-7jszm1nd	51	5	of	of	IN
cord-048471-7jszm1nd	51	6	JV	JV	NNP
cord-048471-7jszm1nd	51	7	polyprotein	polyprotein	NN
cord-048471-7jszm1nd	51	8	processing	processing	NN
cord-048471-7jszm1nd	51	9	indicated	indicate	VBD
cord-048471-7jszm1nd	51	10	that	that	IN
cord-048471-7jszm1nd	51	11	only	only	RB
cord-048471-7jszm1nd	51	12	two	two	CD
cord-048471-7jszm1nd	51	13	major	major	JJ
cord-048471-7jszm1nd	51	14	proteins	protein	NNS
cord-048471-7jszm1nd	51	15	are	be	VBP
cord-048471-7jszm1nd	51	16	synthesised	synthesise	VBN
cord-048471-7jszm1nd	51	17	initially	initially	RB
cord-048471-7jszm1nd	51	18	which	which	WDT
cord-048471-7jszm1nd	51	19	,	,	,
cord-048471-7jszm1nd	51	20	based	base	VBN
cord-048471-7jszm1nd	51	21	on	on	IN
cord-048471-7jszm1nd	51	22	molecular	molecular	JJ
cord-048471-7jszm1nd	51	23	weight	weight	NN
cord-048471-7jszm1nd	51	24	predictions	prediction	NNS
cord-048471-7jszm1nd	51	25	,	,	,
cord-048471-7jszm1nd	51	26	are	be	VBP
cord-048471-7jszm1nd	51	27	the	the	DT
cord-048471-7jszm1nd	51	28	3ABCD	3abcd	CD
cord-048471-7jszm1nd	51	29	(	(	-LRB-
cord-048471-7jszm1nd	51	30	110	110	CD
cord-048471-7jszm1nd	51	31	kDa	kDa	NNPS
cord-048471-7jszm1nd	51	32	)	)	-RRB-
cord-048471-7jszm1nd	51	33	and	and	CC
cord-048471-7jszm1nd	51	34	the	the	DT
cord-048471-7jszm1nd	51	35	2C	2C	NNP
cord-048471-7jszm1nd	51	36	(	(	-LRB-
cord-048471-7jszm1nd	51	37	39	39	CD
cord-048471-7jszm1nd	51	38	kDa	kDa	NNPS
cord-048471-7jszm1nd	51	39	)	)	-RRB-
cord-048471-7jszm1nd	51	40	.	.	.
cord-048471-7jszm1nd	52	1	The	the	DT
cord-048471-7jszm1nd	52	2	lack	lack	NN
cord-048471-7jszm1nd	52	3	of	of	IN
cord-048471-7jszm1nd	52	4	an	an	DT
cord-048471-7jszm1nd	52	5	N	n	CD
cord-048471-7jszm1nd	52	6	-	-	HYPH
cord-048471-7jszm1nd	52	7	terminal	terminal	NN
cord-048471-7jszm1nd	52	8	protein	protein	NN
cord-048471-7jszm1nd	52	9	encoded	encode	VBN
cord-048471-7jszm1nd	52	10	by	by	IN
cord-048471-7jszm1nd	52	11	the	the	DT
cord-048471-7jszm1nd	52	12	JV	JV	NNP
cord-048471-7jszm1nd	52	13	59GS	59gs	CD
cord-048471-7jszm1nd	52	14	,	,	,
cord-048471-7jszm1nd	52	15	predicted	predict	VBD
cord-048471-7jszm1nd	52	16	to	to	TO
cord-048471-7jszm1nd	52	17	be	be	VB
cord-048471-7jszm1nd	52	18	35.3	35.3	CD
cord-048471-7jszm1nd	52	19	kDa	kDa	NNS
cord-048471-7jszm1nd	52	20	,	,	,
cord-048471-7jszm1nd	52	21	is	be	VBZ
cord-048471-7jszm1nd	52	22	unique	unique	JJ
cord-048471-7jszm1nd	52	23	among	among	IN
cord-048471-7jszm1nd	52	24	the	the	DT
cord-048471-7jszm1nd	52	25	noroviruses	noroviruse	NNS
cord-048471-7jszm1nd	52	26	that	that	WDT
cord-048471-7jszm1nd	52	27	have	have	VBP
cord-048471-7jszm1nd	52	28	been	be	VBN
cord-048471-7jszm1nd	52	29	studied	study	VBN
cord-048471-7jszm1nd	52	30	in	in	IN
cord-048471-7jszm1nd	52	31	this	this	DT
cord-048471-7jszm1nd	52	32	way	way	NN
cord-048471-7jszm1nd	52	33	.	.	.
cord-048471-7jszm1nd	53	1	The	the	DT
cord-048471-7jszm1nd	53	2	in	in	IN
cord-048471-7jszm1nd	53	3	vitro	vitro	FW
cord-048471-7jszm1nd	53	4	transcription	transcription	NN
cord-048471-7jszm1nd	53	5	and	and	CC
cord-048471-7jszm1nd	53	6	translation	translation	NN
cord-048471-7jszm1nd	53	7	profile	profile	NN
cord-048471-7jszm1nd	53	8	for	for	IN
cord-048471-7jszm1nd	53	9	JV	JV	NNP
cord-048471-7jszm1nd	53	10	was	be	VBD
cord-048471-7jszm1nd	53	11	therefore	therefore	RB
cord-048471-7jszm1nd	53	12	studied	study	VBN
cord-048471-7jszm1nd	53	13	in	in	IN
cord-048471-7jszm1nd	53	14	more	more	JJR
cord-048471-7jszm1nd	53	15	detail	detail	NN
cord-048471-7jszm1nd	53	16	.	.	.
cord-048471-7jszm1nd	54	1	As	as	IN
cord-048471-7jszm1nd	54	2	initial	initial	JJ
cord-048471-7jszm1nd	54	3	experiments	experiment	NNS
cord-048471-7jszm1nd	54	4	had	have	VBD
cord-048471-7jszm1nd	54	5	analysed	analyse	VBN
cord-048471-7jszm1nd	54	6	TNTH	TNTH	NNP
cord-048471-7jszm1nd	54	7	reactions	reaction	NNS
cord-048471-7jszm1nd	54	8	following	follow	VBG
cord-048471-7jszm1nd	54	9	a	a	DT
cord-048471-7jszm1nd	54	10	1	1	CD
cord-048471-7jszm1nd	54	11	hr	hr	NN
cord-048471-7jszm1nd	54	12	incubation	incubation	NN
cord-048471-7jszm1nd	54	13	,	,	,
cord-048471-7jszm1nd	54	14	reaction	reaction	NN
cord-048471-7jszm1nd	54	15	aliquots	aliquot	NNS
cord-048471-7jszm1nd	54	16	were	be	VBD
cord-048471-7jszm1nd	54	17	harvested	harvest	VBN
cord-048471-7jszm1nd	54	18	at	at	IN
cord-048471-7jszm1nd	54	19	time	time	NN
cord-048471-7jszm1nd	54	20	points	point	NNS
cord-048471-7jszm1nd	54	21	before	before	RB
cord-048471-7jszm1nd	54	22	and	and	CC
cord-048471-7jszm1nd	54	23	after	after	IN
cord-048471-7jszm1nd	54	24	the	the	DT
cord-048471-7jszm1nd	54	25	recommended	recommend	VBN
cord-048471-7jszm1nd	54	26	1	1	CD
cord-048471-7jszm1nd	54	27	hr	hr	NN
cord-048471-7jszm1nd	54	28	incubation	incubation	NN
cord-048471-7jszm1nd	54	29	.	.	.
cord-048471-7jszm1nd	55	1	The	the	DT
cord-048471-7jszm1nd	55	2	results	result	NNS
cord-048471-7jszm1nd	55	3	in	in	IN
cord-048471-7jszm1nd	55	4	Figure	figure	NN
cord-048471-7jszm1nd	55	5	1	1	CD
cord-048471-7jszm1nd	55	6	show	show	VBP
cord-048471-7jszm1nd	55	7	that	that	IN
cord-048471-7jszm1nd	55	8	there	there	EX
cord-048471-7jszm1nd	55	9	are	be	VBP
cord-048471-7jszm1nd	55	10	no	no	DT
cord-048471-7jszm1nd	55	11	major	major	JJ
cord-048471-7jszm1nd	55	12	reaction	reaction	NN
cord-048471-7jszm1nd	55	13	products	product	NNS
cord-048471-7jszm1nd	55	14	synthesised	synthesise	VBN
cord-048471-7jszm1nd	55	15	prior	prior	RB
cord-048471-7jszm1nd	55	16	to	to	IN
cord-048471-7jszm1nd	55	17	the	the	DT
cord-048471-7jszm1nd	55	18	1	1	CD
cord-048471-7jszm1nd	55	19	hr	hr	NN
cord-048471-7jszm1nd	55	20	time	time	NN
cord-048471-7jszm1nd	55	21	point	point	NN
cord-048471-7jszm1nd	55	22	,	,	,
cord-048471-7jszm1nd	55	23	at	at	IN
cord-048471-7jszm1nd	55	24	which	which	WDT
cord-048471-7jszm1nd	55	25	time	time	NN
cord-048471-7jszm1nd	55	26	the	the	DT
cord-048471-7jszm1nd	55	27	3ABCD	3abcd	CD
cord-048471-7jszm1nd	55	28	/	/	SYM
cord-048471-7jszm1nd	55	29	p110	p110	NNP
cord-048471-7jszm1nd	55	30	and	and	CC
cord-048471-7jszm1nd	55	31	2C	2C	NNP
cord-048471-7jszm1nd	55	32	/	/	SYM
cord-048471-7jszm1nd	55	33	p39	p39	NN
cord-048471-7jszm1nd	55	34	proteins	protein	NNS
cord-048471-7jszm1nd	55	35	are	be	VBP
cord-048471-7jszm1nd	55	36	clearly	clearly	RB
cord-048471-7jszm1nd	55	37	visible	visible	JJ
cord-048471-7jszm1nd	55	38	.	.	.
cord-048471-7jszm1nd	56	1	Extended	extend	VBN
cord-048471-7jszm1nd	56	2	incubation	incubation	NN
cord-048471-7jszm1nd	56	3	past	past	IN
cord-048471-7jszm1nd	56	4	the	the	DT
cord-048471-7jszm1nd	56	5	1	1	CD
cord-048471-7jszm1nd	56	6	hr	hr	NN
cord-048471-7jszm1nd	56	7	point	point	NN
cord-048471-7jszm1nd	56	8	resulted	result	VBD
cord-048471-7jszm1nd	56	9	in	in	IN
cord-048471-7jszm1nd	56	10	further	further	JJ
cord-048471-7jszm1nd	56	11	proteolytic	proteolytic	JJ
cord-048471-7jszm1nd	56	12	cleavage	cleavage	NN
cord-048471-7jszm1nd	56	13	of	of	IN
cord-048471-7jszm1nd	56	14	p110	p110	NN
cord-048471-7jszm1nd	56	15	that	that	WDT
cord-048471-7jszm1nd	56	16	coincided	coincide	VBD
cord-048471-7jszm1nd	56	17	with	with	IN
cord-048471-7jszm1nd	56	18	the	the	DT
cord-048471-7jszm1nd	56	19	appearance	appearance	NN
cord-048471-7jszm1nd	56	20	of	of	IN
cord-048471-7jszm1nd	56	21	proteins	protein	NNS
cord-048471-7jszm1nd	56	22	of	of	IN
cord-048471-7jszm1nd	56	23	the	the	DT
cord-048471-7jszm1nd	56	24	following	following	JJ
cord-048471-7jszm1nd	56	25	sizes	size	NNS
cord-048471-7jszm1nd	56	26	:	:	:
cord-048471-7jszm1nd	56	27	86	86	CD
cord-048471-7jszm1nd	56	28	kDa	kDa	NNS
cord-048471-7jszm1nd	56	29	,	,	,
cord-048471-7jszm1nd	56	30	55	55	CD
cord-048471-7jszm1nd	56	31	kDa	kDa	NNS
cord-048471-7jszm1nd	56	32	,	,	,
cord-048471-7jszm1nd	56	33	and	and	CC
cord-048471-7jszm1nd	56	34	51	51	CD
cord-048471-7jszm1nd	56	35	kDa	kDa	NNS
cord-048471-7jszm1nd	56	36	.	.	.
cord-048471-7jszm1nd	57	1	In	in	IN
cord-048471-7jszm1nd	57	2	addition	addition	NN
cord-048471-7jszm1nd	57	3	proteins	protein	NNS
cord-048471-7jszm1nd	57	4	of	of	IN
cord-048471-7jszm1nd	57	5	29	29	CD
cord-048471-7jszm1nd	57	6	kDa	kDa	NNS
cord-048471-7jszm1nd	57	7	,	,	,
cord-048471-7jszm1nd	57	8	22	22	CD
cord-048471-7jszm1nd	57	9	kDa	kDa	NNS
cord-048471-7jszm1nd	57	10	and	and	CC
cord-048471-7jszm1nd	57	11	20	20	CD
cord-048471-7jszm1nd	57	12	kDa	kDa	NNS
cord-048471-7jszm1nd	57	13	were	be	VBD
cord-048471-7jszm1nd	57	14	also	also	RB
cord-048471-7jszm1nd	57	15	visible	visible	JJ
cord-048471-7jszm1nd	57	16	at	at	IN
cord-048471-7jszm1nd	57	17	the	the	DT
cord-048471-7jszm1nd	57	18	24	24	CD
cord-048471-7jszm1nd	57	19	hr	hr	NN
cord-048471-7jszm1nd	57	20	time	time	NN
cord-048471-7jszm1nd	57	21	point	point	NN
cord-048471-7jszm1nd	57	22	(	(	-LRB-
cord-048471-7jszm1nd	57	23	Figure	figure	NN
cord-048471-7jszm1nd	57	24	1	1	CD
cord-048471-7jszm1nd	57	25	,	,	,
cord-048471-7jszm1nd	57	26	lane	lane	NN
cord-048471-7jszm1nd	57	27	7	7	CD
cord-048471-7jszm1nd	57	28	)	)	-RRB-
cord-048471-7jszm1nd	57	29	.	.	.
cord-048471-7jszm1nd	58	1	The	the	DT
cord-048471-7jszm1nd	58	2	only	only	JJ
cord-048471-7jszm1nd	58	3	protein	protein	NN
cord-048471-7jszm1nd	58	4	that	that	WDT
cord-048471-7jszm1nd	58	5	was	be	VBD
cord-048471-7jszm1nd	58	6	consistently	consistently	RB
cord-048471-7jszm1nd	58	7	visible	visible	JJ
cord-048471-7jszm1nd	58	8	following	follow	VBG
cord-048471-7jszm1nd	58	9	the	the	DT
cord-048471-7jszm1nd	58	10	1	1	CD
cord-048471-7jszm1nd	58	11	hr	hr	NN
cord-048471-7jszm1nd	58	12	time	time	NN
cord-048471-7jszm1nd	58	13	point	point	NN
cord-048471-7jszm1nd	58	14	was	be	VBD
cord-048471-7jszm1nd	58	15	the	the	DT
cord-048471-7jszm1nd	58	16	2C	2C	NNP
cord-048471-7jszm1nd	58	17	/	/	SYM
cord-048471-7jszm1nd	58	18	p39	p39	NN
cord-048471-7jszm1nd	58	19	protein	protein	NN
cord-048471-7jszm1nd	58	20	.	.	.
cord-048471-7jszm1nd	59	1	Despite	despite	IN
cord-048471-7jszm1nd	59	2	prolonged	prolonged	JJ
cord-048471-7jszm1nd	59	3	incubation	incubation	NN
cord-048471-7jszm1nd	59	4	there	there	EX
cord-048471-7jszm1nd	59	5	was	be	VBD
cord-048471-7jszm1nd	59	6	no	no	DT
cord-048471-7jszm1nd	59	7	indication	indication	NN
cord-048471-7jszm1nd	59	8	that	that	IN
cord-048471-7jszm1nd	59	9	the	the	DT
cord-048471-7jszm1nd	59	10	N	n	CD
cord-048471-7jszm1nd	59	11	-	-	HYPH
cord-048471-7jszm1nd	59	12	terminal	terminal	NN
cord-048471-7jszm1nd	59	13	/	/	SYM
cord-048471-7jszm1nd	59	14	p35	p35	CD
cord-048471-7jszm1nd	59	15	protein	protein	NN
cord-048471-7jszm1nd	59	16	was	be	VBD
cord-048471-7jszm1nd	59	17	synthesised	synthesise	VBN
cord-048471-7jszm1nd	59	18	.	.	.
cord-048471-7jszm1nd	60	1	A	a	DT
cord-048471-7jszm1nd	60	2	comprehensive	comprehensive	JJ
cord-048471-7jszm1nd	60	3	study	study	NN
cord-048471-7jszm1nd	60	4	of	of	IN
cord-048471-7jszm1nd	60	5	polyprotein	polyprotein	JJ
cord-048471-7jszm1nd	60	6	processing	processing	NN
cord-048471-7jszm1nd	60	7	within	within	IN
cord-048471-7jszm1nd	60	8	the	the	DT
cord-048471-7jszm1nd	60	9	murine	murine	JJ
cord-048471-7jszm1nd	60	10	norovirus	norovirus	NN
cord-048471-7jszm1nd	60	11	(	(	-LRB-
cord-048471-7jszm1nd	60	12	MNV	MNV	NNP
cord-048471-7jszm1nd	60	13	)	)	-RRB-
cord-048471-7jszm1nd	60	14	suggests	suggest	VBZ
cord-048471-7jszm1nd	60	15	likely	likely	JJ
cord-048471-7jszm1nd	60	16	identities	identity	NNS
cord-048471-7jszm1nd	60	17	for	for	IN
cord-048471-7jszm1nd	60	18	the	the	DT
cord-048471-7jszm1nd	60	19	equivalent	equivalent	JJ
cord-048471-7jszm1nd	60	20	proteins	protein	NNS
cord-048471-7jszm1nd	60	21	in	in	IN
cord-048471-7jszm1nd	60	22	the	the	DT
cord-048471-7jszm1nd	60	23	similar	similar	JJ
cord-048471-7jszm1nd	60	24	profile	profile	NN
cord-048471-7jszm1nd	60	25	for	for	IN
cord-048471-7jszm1nd	60	26	JV	JV	NNP
cord-048471-7jszm1nd	60	27	[	[	-LRB-
cord-048471-7jszm1nd	60	28	12	12	CD
cord-048471-7jszm1nd	60	29	]	]	-RRB-
cord-048471-7jszm1nd	60	30	.	.	.
cord-048471-7jszm1nd	61	1	Using	use	VBG
cord-048471-7jszm1nd	61	2	region	region	NN
cord-048471-7jszm1nd	61	3	specific	specific	JJ
cord-048471-7jszm1nd	61	4	antisera	antisera	NN
cord-048471-7jszm1nd	61	5	the	the	DT
cord-048471-7jszm1nd	61	6	authors	author	NNS
cord-048471-7jszm1nd	61	7	were	be	VBD
cord-048471-7jszm1nd	61	8	able	able	JJ
cord-048471-7jszm1nd	61	9	to	to	TO
cord-048471-7jszm1nd	61	10	identify	identify	VB
cord-048471-7jszm1nd	61	11	p110	p110	NNS
cord-048471-7jszm1nd	61	12	as	as	IN
cord-048471-7jszm1nd	61	13	the	the	DT
cord-048471-7jszm1nd	61	14	3ABCD	3abcd	CD
cord-048471-7jszm1nd	61	15	uncleaved	uncleaved	JJ
cord-048471-7jszm1nd	61	16	precursor	precursor	NN
cord-048471-7jszm1nd	61	17	,	,	,
cord-048471-7jszm1nd	61	18	p90	p90	NN
cord-048471-7jszm1nd	61	19	as	as	IN
cord-048471-7jszm1nd	61	20	the	the	DT
cord-048471-7jszm1nd	61	21	3BCD	3BCD	NNP
cord-048471-7jszm1nd	61	22	,	,	,
cord-048471-7jszm1nd	61	23	p57.5	p57.5	XX
cord-048471-7jszm1nd	61	24	as	as	IN
cord-048471-7jszm1nd	61	25	the	the	DT
cord-048471-7jszm1nd	61	26	3D	3d	JJ
cord-048471-7jszm1nd	61	27	-	-	HYPH
cord-048471-7jszm1nd	61	28	like	like	JJ
cord-048471-7jszm1nd	61	29	polymerase	polymerase	NN
cord-048471-7jszm1nd	61	30	,	,	,
cord-048471-7jszm1nd	61	31	p52	p52	NN
cord-048471-7jszm1nd	61	32	as	as	IN
cord-048471-7jszm1nd	61	33	a	a	DT
cord-048471-7jszm1nd	61	34	3ABC	3abc	NN
cord-048471-7jszm1nd	61	35	precursor	precursor	NN
cord-048471-7jszm1nd	61	36	and	and	CC
cord-048471-7jszm1nd	61	37	p40	p40	NN
cord-048471-7jszm1nd	61	38	as	as	IN
cord-048471-7jszm1nd	61	39	the	the	DT
cord-048471-7jszm1nd	61	40	2Clike	2clike	JJ
cord-048471-7jszm1nd	61	41	NTPase	NTPase	NNP
cord-048471-7jszm1nd	61	42	,	,	,
cord-048471-7jszm1nd	61	43	which	which	WDT
cord-048471-7jszm1nd	61	44	was	be	VBD
cord-048471-7jszm1nd	61	45	determined	determine	VBN
cord-048471-7jszm1nd	61	46	by	by	IN
cord-048471-7jszm1nd	61	47	mutagenesis	mutagenesis	NN
cord-048471-7jszm1nd	61	48	and	and	CC
cord-048471-7jszm1nd	61	49	microsequencing	microsequence	VBG
cord-048471-7jszm1nd	61	50	experiments	experiment	NNS
cord-048471-7jszm1nd	61	51	.	.	.
cord-048471-7jszm1nd	62	1	The	the	DT
cord-048471-7jszm1nd	62	2	19	19	CD
cord-048471-7jszm1nd	62	3	kDa	kDa	NNP
cord-048471-7jszm1nd	62	4	protein	protein	NN
cord-048471-7jszm1nd	62	5	was	be	VBD
cord-048471-7jszm1nd	62	6	identified	identify	VBN
cord-048471-7jszm1nd	62	7	as	as	IN
cord-048471-7jszm1nd	62	8	the	the	DT
cord-048471-7jszm1nd	62	9	3C	3c	NN
cord-048471-7jszm1nd	62	10	-	-	HYPH
cord-048471-7jszm1nd	62	11	like	like	JJ
cord-048471-7jszm1nd	62	12	protease	protease	NN
cord-048471-7jszm1nd	62	13	.	.	.
cord-048471-7jszm1nd	63	1	The	the	DT
cord-048471-7jszm1nd	63	2	antisera	antisera	NN
cord-048471-7jszm1nd	63	3	used	use	VBD
cord-048471-7jszm1nd	63	4	to	to	TO
cord-048471-7jszm1nd	63	5	detect	detect	VB
cord-048471-7jszm1nd	63	6	the	the	DT
cord-048471-7jszm1nd	63	7	MNV	MNV	NNP
cord-048471-7jszm1nd	63	8	Nterm	Nterm	NNP
cord-048471-7jszm1nd	63	9	protein	protein	NN
cord-048471-7jszm1nd	63	10	recognised	recognise	VBD
cord-048471-7jszm1nd	63	11	3	3	CD
cord-048471-7jszm1nd	63	12	products	product	NNS
cord-048471-7jszm1nd	63	13	;	;	:
cord-048471-7jszm1nd	63	14	one	one	CD
cord-048471-7jszm1nd	63	15	was	be	VBD
cord-048471-7jszm1nd	63	16	the	the	DT
cord-048471-7jszm1nd	63	17	predicted	predict	VBN
cord-048471-7jszm1nd	63	18	molecular	molecular	JJ
cord-048471-7jszm1nd	63	19	weight	weight	NN
cord-048471-7jszm1nd	63	20	at	at	IN
cord-048471-7jszm1nd	63	21	39	39	CD
cord-048471-7jszm1nd	63	22	kDa	kDa	NNS
cord-048471-7jszm1nd	63	23	and	and	CC
cord-048471-7jszm1nd	63	24	the	the	DT
cord-048471-7jszm1nd	63	25	other	other	JJ
cord-048471-7jszm1nd	63	26	two	two	CD
cord-048471-7jszm1nd	63	27	bands	band	NNS
cord-048471-7jszm1nd	63	28	migrated	migrate	VBD
cord-048471-7jszm1nd	63	29	as	as	IN
cord-048471-7jszm1nd	63	30	a	a	DT
cord-048471-7jszm1nd	63	31	45	45	CD
cord-048471-7jszm1nd	63	32	kDa	kDa	NNS
cord-048471-7jszm1nd	63	33	doublet	doublet	NN
cord-048471-7jszm1nd	63	34	.	.	.
cord-048471-7jszm1nd	64	1	The	the	DT
cord-048471-7jszm1nd	64	2	59GS	59gs	CD
cord-048471-7jszm1nd	64	3	region	region	NN
cord-048471-7jszm1nd	64	4	of	of	IN
cord-048471-7jszm1nd	64	5	JV	JV	NNP
cord-048471-7jszm1nd	64	6	is	be	VBZ
cord-048471-7jszm1nd	64	7	highly	highly	RB
cord-048471-7jszm1nd	64	8	divergent	divergent	JJ
cord-048471-7jszm1nd	64	9	compared	compare	VBN
cord-048471-7jszm1nd	64	10	to	to	IN
cord-048471-7jszm1nd	64	11	other	other	JJ
cord-048471-7jszm1nd	64	12	noroviruses	noroviruse	NNS
cord-048471-7jszm1nd	64	13	,	,	,
cord-048471-7jszm1nd	64	14	mainly	mainly	RB
cord-048471-7jszm1nd	64	15	due	due	IN
cord-048471-7jszm1nd	64	16	to	to	IN
cord-048471-7jszm1nd	64	17	the	the	DT
cord-048471-7jszm1nd	64	18	relatively	relatively	RB
cord-048471-7jszm1nd	64	19	high	high	JJ
cord-048471-7jszm1nd	64	20	cytosine	cytosine	NN
cord-048471-7jszm1nd	64	21	content	content	NN
cord-048471-7jszm1nd	64	22	(	(	-LRB-
cord-048471-7jszm1nd	64	23	32	32	CD
cord-048471-7jszm1nd	64	24	%	%	NN
cord-048471-7jszm1nd	64	25	)	)	-RRB-
cord-048471-7jszm1nd	64	26	,	,	,
cord-048471-7jszm1nd	64	27	which	which	WDT
cord-048471-7jszm1nd	64	28	contributes	contribute	VBZ
cord-048471-7jszm1nd	64	29	to	to	IN
cord-048471-7jszm1nd	64	30	an	an	DT
cord-048471-7jszm1nd	64	31	overall	overall	JJ
cord-048471-7jszm1nd	64	32	G	g	NN
cord-048471-7jszm1nd	64	33	/	/	SYM
cord-048471-7jszm1nd	64	34	C	c	NN
cord-048471-7jszm1nd	64	35	content	content	NN
cord-048471-7jszm1nd	64	36	of	of	IN
cord-048471-7jszm1nd	64	37	58	58	CD
cord-048471-7jszm1nd	64	38	%	%	NN
cord-048471-7jszm1nd	64	39	.	.	.
cord-048471-7jszm1nd	65	1	There	there	EX
cord-048471-7jszm1nd	65	2	are	be	VBP
cord-048471-7jszm1nd	65	3	many	many	JJ
cord-048471-7jszm1nd	65	4	polypyrimidine	polypyrimidine	JJ
cord-048471-7jszm1nd	65	5	tracts	tract	NNS
cord-048471-7jszm1nd	65	6	within	within	IN
cord-048471-7jszm1nd	65	7	the	the	DT
cord-048471-7jszm1nd	65	8	sequence	sequence	NN
cord-048471-7jszm1nd	65	9	,	,	,
cord-048471-7jszm1nd	65	10	potentially	potentially	RB
cord-048471-7jszm1nd	65	11	yielding	yield	VBG
cord-048471-7jszm1nd	65	12	a	a	DT
cord-048471-7jszm1nd	65	13	relatively	relatively	RB
cord-048471-7jszm1nd	65	14	high	high	JJ
cord-048471-7jszm1nd	65	15	degree	degree	NN
cord-048471-7jszm1nd	65	16	of	of	IN
cord-048471-7jszm1nd	65	17	RNA	RNA	NNP
cord-048471-7jszm1nd	65	18	secondary	secondary	JJ
cord-048471-7jszm1nd	65	19	structure	structure	NN
cord-048471-7jszm1nd	65	20	.	.	.
cord-048471-7jszm1nd	66	1	Previous	previous	JJ
cord-048471-7jszm1nd	66	2	studies	study	NNS
cord-048471-7jszm1nd	66	3	have	have	VBP
cord-048471-7jszm1nd	66	4	described	describe	VBN
cord-048471-7jszm1nd	66	5	potential	potential	JJ
cord-048471-7jszm1nd	66	6	secondary	secondary	JJ
cord-048471-7jszm1nd	66	7	RNA	RNA	NNP
cord-048471-7jszm1nd	66	8	structure	structure	NN
cord-048471-7jszm1nd	66	9	and	and	CC
cord-048471-7jszm1nd	66	10	interaction	interaction	NN
cord-048471-7jszm1nd	66	11	with	with	IN
cord-048471-7jszm1nd	66	12	proteins	protein	NNS
cord-048471-7jszm1nd	66	13	involved	involve	VBN
cord-048471-7jszm1nd	66	14	with	with	IN
cord-048471-7jszm1nd	66	15	IRESmediated	iresmediate	VBN
cord-048471-7jszm1nd	66	16	translation	translation	NN
cord-048471-7jszm1nd	66	17	within	within	IN
cord-048471-7jszm1nd	66	18	the	the	DT
cord-048471-7jszm1nd	66	19	59	59	CD
cord-048471-7jszm1nd	66	20	genomic	genomic	JJ
cord-048471-7jszm1nd	66	21	region	region	NN
cord-048471-7jszm1nd	66	22	of	of	IN
cord-048471-7jszm1nd	66	23	Norwalk	Norwalk	NNP
cord-048471-7jszm1nd	66	24	virus	virus	NN
cord-048471-7jszm1nd	66	25	[	[	-LRB-
cord-048471-7jszm1nd	66	26	18	18	CD
cord-048471-7jszm1nd	66	27	,	,	,
cord-048471-7jszm1nd	66	28	24	24	CD
cord-048471-7jszm1nd	66	29	]	]	-RRB-
cord-048471-7jszm1nd	66	30	.	.	.
cord-048471-7jszm1nd	67	1	It	-PRON-	PRP
cord-048471-7jszm1nd	67	2	was	be	VBD
cord-048471-7jszm1nd	67	3	of	of	IN
cord-048471-7jszm1nd	67	4	interest	interest	NN
cord-048471-7jszm1nd	67	5	therefore	therefore	RB
cord-048471-7jszm1nd	67	6	,	,	,
cord-048471-7jszm1nd	67	7	based	base	VBN
cord-048471-7jszm1nd	67	8	on	on	IN
cord-048471-7jszm1nd	67	9	these	these	DT
cord-048471-7jszm1nd	67	10	findings	finding	NNS
cord-048471-7jszm1nd	67	11	,	,	,
cord-048471-7jszm1nd	67	12	to	to	TO
cord-048471-7jszm1nd	67	13	ascertain	ascertain	VB
cord-048471-7jszm1nd	67	14	whether	whether	IN
cord-048471-7jszm1nd	67	15	or	or	CC
cord-048471-7jszm1nd	67	16	not	not	RB
cord-048471-7jszm1nd	67	17	the	the	DT
cord-048471-7jszm1nd	67	18	59GS	59gs	CD
cord-048471-7jszm1nd	67	19	of	of	IN
cord-048471-7jszm1nd	67	20	JV	JV	NNP
cord-048471-7jszm1nd	67	21	possessed	possess	VBD
cord-048471-7jszm1nd	67	22	IRES	IRES	NNP
cord-048471-7jszm1nd	67	23	-	-	HYPH
cord-048471-7jszm1nd	67	24	like	like	JJ
cord-048471-7jszm1nd	67	25	properties	property	NNS
cord-048471-7jszm1nd	67	26	within	within	IN
cord-048471-7jszm1nd	67	27	the	the	DT
cord-048471-7jszm1nd	67	28	context	context	NN
cord-048471-7jszm1nd	67	29	of	of	IN
cord-048471-7jszm1nd	67	30	a	a	DT
cord-048471-7jszm1nd	67	31	'	'	``
cord-048471-7jszm1nd	67	32	Bi	Bi	NNP
cord-048471-7jszm1nd	67	33	-	-	HYPH
cord-048471-7jszm1nd	67	34	cistronic	cistronic	NNP
cord-048471-7jszm1nd	67	35	'	'	POS
cord-048471-7jszm1nd	67	36	expression	expression	NN
cord-048471-7jszm1nd	67	37	system	system	NN
cord-048471-7jszm1nd	67	38	,	,	,
cord-048471-7jszm1nd	67	39	independently	independently	RB
cord-048471-7jszm1nd	67	40	of	of	IN
cord-048471-7jszm1nd	67	41	other	other	JJ
cord-048471-7jszm1nd	67	42	viral	viral	JJ
cord-048471-7jszm1nd	67	43	proteins	protein	NNS
cord-048471-7jszm1nd	67	44	,	,	,
cord-048471-7jszm1nd	67	45	including	include	VBG
cord-048471-7jszm1nd	67	46	the	the	DT
cord-048471-7jszm1nd	67	47	VPg	VPg	NNS
cord-048471-7jszm1nd	67	48	which	which	WDT
cord-048471-7jszm1nd	67	49	,	,	,
cord-048471-7jszm1nd	67	50	in	in	IN
cord-048471-7jszm1nd	67	51	other	other	JJ
cord-048471-7jszm1nd	67	52	caliciviruses	caliciviruse	NNS
cord-048471-7jszm1nd	67	53	,	,	,
cord-048471-7jszm1nd	67	54	has	have	VBZ
cord-048471-7jszm1nd	67	55	been	be	VBN
cord-048471-7jszm1nd	67	56	shown	show	VBN
cord-048471-7jszm1nd	67	57	to	to	TO
cord-048471-7jszm1nd	67	58	be	be	VB
cord-048471-7jszm1nd	67	59	associated	associate	VBN
cord-048471-7jszm1nd	67	60	with	with	IN
cord-048471-7jszm1nd	67	61	translation	translation	NN
cord-048471-7jszm1nd	67	62	initiation	initiation	NN
cord-048471-7jszm1nd	67	63	factors	factor	NNS
cord-048471-7jszm1nd	67	64	[	[	-LRB-
cord-048471-7jszm1nd	67	65	25	25	CD
cord-048471-7jszm1nd	67	66	,	,	,
cord-048471-7jszm1nd	67	67	26	26	CD
cord-048471-7jszm1nd	67	68	]	]	-RRB-
cord-048471-7jszm1nd	67	69	.	.	.
cord-048471-7jszm1nd	68	1	Traditionally	traditionally	RB
cord-048471-7jszm1nd	68	2	the	the	DT
cord-048471-7jszm1nd	68	3	bi	bi	JJ
cord-048471-7jszm1nd	68	4	-	-	HYPH
cord-048471-7jszm1nd	68	5	cistronic	cistronic	JJ
cord-048471-7jszm1nd	68	6	vector	vector	NN
cord-048471-7jszm1nd	68	7	system	system	NN
cord-048471-7jszm1nd	68	8	has	have	VBZ
cord-048471-7jszm1nd	68	9	been	be	VBN
cord-048471-7jszm1nd	68	10	used	use	VBN
cord-048471-7jszm1nd	68	11	to	to	TO
cord-048471-7jszm1nd	68	12	define	define	VB
cord-048471-7jszm1nd	68	13	potential	potential	JJ
cord-048471-7jszm1nd	68	14	IRES	IRES	NNP
cord-048471-7jszm1nd	68	15	-	-	HYPH
cord-048471-7jszm1nd	68	16	like	like	JJ
cord-048471-7jszm1nd	68	17	sequences	sequence	NNS
cord-048471-7jszm1nd	68	18	from	from	IN
cord-048471-7jszm1nd	68	19	a	a	DT
cord-048471-7jszm1nd	68	20	variety	variety	NN
cord-048471-7jszm1nd	68	21	of	of	IN
cord-048471-7jszm1nd	68	22	viral	viral	JJ
cord-048471-7jszm1nd	68	23	and	and	CC
cord-048471-7jszm1nd	68	24	cellular	cellular	JJ
cord-048471-7jszm1nd	68	25	mRNAs	mrna	NNS
cord-048471-7jszm1nd	68	26	,	,	,
cord-048471-7jszm1nd	68	27	and	and	CC
cord-048471-7jszm1nd	68	28	is	be	VBZ
cord-048471-7jszm1nd	68	29	recognized	recognize	VBN
cord-048471-7jszm1nd	68	30	as	as	IN
cord-048471-7jszm1nd	68	31	being	be	VBG
cord-048471-7jszm1nd	68	32	the	the	DT
cord-048471-7jszm1nd	68	33	standard	standard	JJ
cord-048471-7jszm1nd	68	34	test	test	NN
cord-048471-7jszm1nd	68	35	for	for	IN
cord-048471-7jszm1nd	68	36	this	this	DT
cord-048471-7jszm1nd	68	37	function	function	NN
cord-048471-7jszm1nd	68	38	[	[	-LRB-
cord-048471-7jszm1nd	68	39	27	27	CD
cord-048471-7jszm1nd	68	40	]	]	-RRB-
cord-048471-7jszm1nd	68	41	.	.	.
cord-048471-7jszm1nd	69	1	A	a	DT
cord-048471-7jszm1nd	69	2	bi	bi	JJ
cord-048471-7jszm1nd	69	3	-	-	HYPH
cord-048471-7jszm1nd	69	4	cistronic	cistronic	JJ
cord-048471-7jszm1nd	69	5	vector	vector	NN
cord-048471-7jszm1nd	69	6	is	be	VBZ
cord-048471-7jszm1nd	69	7	comprised	comprise	VBN
cord-048471-7jszm1nd	69	8	of	of	IN
cord-048471-7jszm1nd	69	9	a	a	DT
cord-048471-7jszm1nd	69	10	59	59	CD
cord-048471-7jszm1nd	69	11	and	and	CC
cord-048471-7jszm1nd	69	12	39	39	CD
cord-048471-7jszm1nd	69	13	cistron	cistron	NN
cord-048471-7jszm1nd	69	14	;	;	:
cord-048471-7jszm1nd	69	15	translation	translation	NN
cord-048471-7jszm1nd	69	16	of	of	IN
cord-048471-7jszm1nd	69	17	the	the	DT
cord-048471-7jszm1nd	69	18	59	59	CD
cord-048471-7jszm1nd	69	19	cistron	cistron	NN
cord-048471-7jszm1nd	69	20	being	be	VBG
cord-048471-7jszm1nd	69	21	cap	cap	NN
cord-048471-7jszm1nd	69	22	-	-	HYPH
cord-048471-7jszm1nd	69	23	dependent	dependent	JJ
cord-048471-7jszm1nd	69	24	and	and	CC
cord-048471-7jszm1nd	69	25	translation	translation	NN
cord-048471-7jszm1nd	69	26	of	of	IN
cord-048471-7jszm1nd	69	27	the	the	DT
cord-048471-7jszm1nd	69	28	39	39	CD
cord-048471-7jszm1nd	69	29	cistron	cistron	NN
cord-048471-7jszm1nd	69	30	regulated	regulate	VBN
cord-048471-7jszm1nd	69	31	by	by	IN
cord-048471-7jszm1nd	69	32	the	the	DT
cord-048471-7jszm1nd	69	33	putative	putative	JJ
cord-048471-7jszm1nd	69	34	IRES	IRES	NNP
cord-048471-7jszm1nd	69	35	-	-	HYPH
cord-048471-7jszm1nd	69	36	like	like	JJ
cord-048471-7jszm1nd	69	37	sequence	sequence	NN
cord-048471-7jszm1nd	69	38	.	.	.
cord-048471-7jszm1nd	70	1	Thus	thus	RB
cord-048471-7jszm1nd	70	2	,	,	,
cord-048471-7jszm1nd	70	3	if	if	IN
cord-048471-7jszm1nd	70	4	the	the	DT
cord-048471-7jszm1nd	70	5	39	39	CD
cord-048471-7jszm1nd	70	6	cistron	cistron	NN
cord-048471-7jszm1nd	70	7	is	be	VBZ
cord-048471-7jszm1nd	70	8	translated	translate	VBN
cord-048471-7jszm1nd	70	9	in	in	IN
cord-048471-7jszm1nd	70	10	addition	addition	NN
cord-048471-7jszm1nd	70	11	to	to	IN
cord-048471-7jszm1nd	70	12	the	the	DT
cord-048471-7jszm1nd	70	13	59	59	CD
cord-048471-7jszm1nd	70	14	cistron	cistron	NN
cord-048471-7jszm1nd	70	15	then	then	RB
cord-048471-7jszm1nd	70	16	the	the	DT
cord-048471-7jszm1nd	70	17	sequence	sequence	NN
cord-048471-7jszm1nd	70	18	of	of	IN
cord-048471-7jszm1nd	70	19	interest	interest	NN
cord-048471-7jszm1nd	70	20	is	be	VBZ
cord-048471-7jszm1nd	70	21	said	say	VBN
cord-048471-7jszm1nd	70	22	to	to	TO
cord-048471-7jszm1nd	70	23	have	have	VB
cord-048471-7jszm1nd	70	24	IRES	ire	VBN
cord-048471-7jszm1nd	70	25	-	-	HYPH
cord-048471-7jszm1nd	70	26	like	like	JJ
cord-048471-7jszm1nd	70	27	properties	property	NNS
cord-048471-7jszm1nd	70	28	,	,	,
cord-048471-7jszm1nd	70	29	as	as	IN
cord-048471-7jszm1nd	70	30	translation	translation	NN
cord-048471-7jszm1nd	70	31	is	be	VBZ
cord-048471-7jszm1nd	70	32	initiating	initiate	VBG
cord-048471-7jszm1nd	70	33	internally	internally	RB
cord-048471-7jszm1nd	70	34	.	.	.
cord-048471-7jszm1nd	71	1	To	to	TO
cord-048471-7jszm1nd	71	2	test	test	VB
cord-048471-7jszm1nd	71	3	for	for	IN
cord-048471-7jszm1nd	71	4	IRES	IRES	NNP
cord-048471-7jszm1nd	71	5	-	-	HYPH
cord-048471-7jszm1nd	71	6	like	like	JJ
cord-048471-7jszm1nd	71	7	function	function	NN
cord-048471-7jszm1nd	71	8	in	in	IN
cord-048471-7jszm1nd	71	9	JV	JV	NNP
cord-048471-7jszm1nd	71	10	,	,	,
cord-048471-7jszm1nd	71	11	bi	bi	NNP
cord-048471-7jszm1nd	71	12	-	-	HYPH
cord-048471-7jszm1nd	71	13	cistronic	cistronic	JJ
cord-048471-7jszm1nd	71	14	constructs	construct	NNS
cord-048471-7jszm1nd	71	15	were	be	VBD
cord-048471-7jszm1nd	71	16	made	make	VBN
cord-048471-7jszm1nd	71	17	with	with	IN
cord-048471-7jszm1nd	71	18	a	a	DT
cord-048471-7jszm1nd	71	19	cap	cap	NN
cord-048471-7jszm1nd	71	20	dependent	dependent	JJ
cord-048471-7jszm1nd	71	21	59	59	CD
cord-048471-7jszm1nd	71	22	EGFP	EGFP	NNP
cord-048471-7jszm1nd	71	23	cistron	cistron	NN
cord-048471-7jszm1nd	71	24	and	and	CC
cord-048471-7jszm1nd	71	25	a	a	DT
cord-048471-7jszm1nd	71	26	39	39	CD
cord-048471-7jszm1nd	71	27	lacZ	lacZ	NNP
cord-048471-7jszm1nd	71	28	cistron	cistron	NN
cord-048471-7jszm1nd	71	29	under	under	IN
cord-048471-7jszm1nd	71	30	the	the	DT
cord-048471-7jszm1nd	71	31	translational	translational	JJ
cord-048471-7jszm1nd	71	32	control	control	NN
cord-048471-7jszm1nd	71	33	of	of	IN
cord-048471-7jszm1nd	71	34	either	either	CC
cord-048471-7jszm1nd	71	35	the	the	DT
cord-048471-7jszm1nd	71	36	JV	JV	NNP
cord-048471-7jszm1nd	71	37	59GS	59gs	CD
cord-048471-7jszm1nd	71	38	(	(	-LRB-
cord-048471-7jszm1nd	71	39	pEGFP	pEGFP	NNP
cord-048471-7jszm1nd	71	40	-	-	HYPH
cord-048471-7jszm1nd	71	41	C1/	C1/	NNP
cord-048471-7jszm1nd	71	42	JV59GS	JV59GS	NNP
cord-048471-7jszm1nd	71	43	/	/	SYM
cord-048471-7jszm1nd	71	44	lacZ	lacZ	NNP
cord-048471-7jszm1nd	71	45	)	)	-RRB-
cord-048471-7jszm1nd	71	46	or	or	CC
cord-048471-7jszm1nd	71	47	an	an	DT
cord-048471-7jszm1nd	71	48	authentic	authentic	JJ
cord-048471-7jszm1nd	71	49	EMCV	EMCV	NNP
cord-048471-7jszm1nd	71	50	IRES	IRES	NNP
cord-048471-7jszm1nd	71	51	(	(	-LRB-
cord-048471-7jszm1nd	71	52	pEGFP	pEGFP	NNP
cord-048471-7jszm1nd	71	53	-	-	HYPH
cord-048471-7jszm1nd	71	54	C1	C1	NNP
cord-048471-7jszm1nd	71	55	/	/	SYM
cord-048471-7jszm1nd	71	56	IRES/	IRES/	NNP
cord-048471-7jszm1nd	71	57	lacZ	lacz	POS
cord-048471-7jszm1nd	71	58	)	)	-RRB-
cord-048471-7jszm1nd	71	59	.	.	.
cord-048471-7jszm1nd	72	1	CRFK	CRFK	NNP
cord-048471-7jszm1nd	72	2	cells	cell	NNS
cord-048471-7jszm1nd	72	3	were	be	VBD
cord-048471-7jszm1nd	72	4	transfected	transfecte	VBN
cord-048471-7jszm1nd	72	5	with	with	IN
cord-048471-7jszm1nd	72	6	the	the	DT
cord-048471-7jszm1nd	72	7	bi	bi	JJ
cord-048471-7jszm1nd	72	8	-	-	HYPH
cord-048471-7jszm1nd	72	9	cistronic	cistronic	JJ
cord-048471-7jszm1nd	72	10	constructs	construct	NNS
cord-048471-7jszm1nd	72	11	and	and	CC
cord-048471-7jszm1nd	72	12	,	,	,
cord-048471-7jszm1nd	72	13	following	follow	VBG
cord-048471-7jszm1nd	72	14	incubation	incubation	NN
cord-048471-7jszm1nd	72	15	,	,	,
cord-048471-7jszm1nd	72	16	were	be	VBD
cord-048471-7jszm1nd	72	17	assayed	assay	VBN
cord-048471-7jszm1nd	72	18	for	for	IN
cord-048471-7jszm1nd	72	19	EGFP	EGFP	NNP
cord-048471-7jszm1nd	72	20	and	and	CC
cord-048471-7jszm1nd	72	21	lacZ	lacZ	NNP
cord-048471-7jszm1nd	72	22	expression	expression	NN
cord-048471-7jszm1nd	72	23	.	.	.
cord-048471-7jszm1nd	73	1	Both	both	DT
cord-048471-7jszm1nd	73	2	constructs	construct	NNS
cord-048471-7jszm1nd	73	3	were	be	VBD
cord-048471-7jszm1nd	73	4	able	able	JJ
cord-048471-7jszm1nd	73	5	to	to	TO
cord-048471-7jszm1nd	73	6	direct	direct	VB
cord-048471-7jszm1nd	73	7	translation	translation	NN
cord-048471-7jszm1nd	73	8	of	of	IN
cord-048471-7jszm1nd	73	9	the	the	DT
cord-048471-7jszm1nd	73	10	EGFP	EGFP	NNP
cord-048471-7jszm1nd	73	11	cistron	cistron	NN
cord-048471-7jszm1nd	73	12	effectively	effectively	RB
cord-048471-7jszm1nd	73	13	as	as	IN
cord-048471-7jszm1nd	73	14	expected	expect	VBN
cord-048471-7jszm1nd	73	15	(	(	-LRB-
cord-048471-7jszm1nd	73	16	Figure	figure	NN
cord-048471-7jszm1nd	73	17	2a	2a	CD
cord-048471-7jszm1nd	73	18	and	and	CC
cord-048471-7jszm1nd	73	19	Figure2d	Figure2d	NNP
cord-048471-7jszm1nd	73	20	)	)	-RRB-
cord-048471-7jszm1nd	73	21	.	.	.
cord-048471-7jszm1nd	74	1	The	the	DT
cord-048471-7jszm1nd	74	2	use	use	NN
cord-048471-7jszm1nd	74	3	of	of	IN
cord-048471-7jszm1nd	74	4	an	an	DT
cord-048471-7jszm1nd	74	5	authentic	authentic	JJ
cord-048471-7jszm1nd	74	6	EMCV	EMCV	NNP
cord-048471-7jszm1nd	74	7	IRES	IRES	NNP
cord-048471-7jszm1nd	74	8	to	to	TO
cord-048471-7jszm1nd	74	9	direct	direct	JJ
cord-048471-7jszm1nd	74	10	translation	translation	NN
cord-048471-7jszm1nd	74	11	of	of	IN
cord-048471-7jszm1nd	74	12	the	the	DT
cord-048471-7jszm1nd	74	13	lacZ	lacZ	NNP
cord-048471-7jszm1nd	74	14	cistron	cistron	NN
cord-048471-7jszm1nd	74	15	was	be	VBD
cord-048471-7jszm1nd	74	16	also	also	RB
cord-048471-7jszm1nd	74	17	effective	effective	JJ
cord-048471-7jszm1nd	74	18	(	(	-LRB-
cord-048471-7jszm1nd	74	19	Figure	figure	NN
cord-048471-7jszm1nd	74	20	2e	2e	CD
cord-048471-7jszm1nd	74	21	)	)	-RRB-
cord-048471-7jszm1nd	74	22	,	,	,
cord-048471-7jszm1nd	74	23	with	with	IN
cord-048471-7jszm1nd	74	24	levels	level	NNS
cord-048471-7jszm1nd	74	25	of	of	IN
cord-048471-7jszm1nd	74	26	b	b	NN
cord-048471-7jszm1nd	74	27	-	-	HYPH
cord-048471-7jszm1nd	74	28	galactosidase	galactosidase	NN
cord-048471-7jszm1nd	74	29	activity	activity	NN
cord-048471-7jszm1nd	74	30	comparable	comparable	JJ
cord-048471-7jszm1nd	74	31	to	to	IN
cord-048471-7jszm1nd	74	32	those	those	DT
cord-048471-7jszm1nd	74	33	of	of	IN
cord-048471-7jszm1nd	74	34	the	the	DT
cord-048471-7jszm1nd	74	35	b	b	NN
cord-048471-7jszm1nd	74	36	-	-	HYPH
cord-048471-7jszm1nd	74	37	galactosidase	galactosidase	NN
cord-048471-7jszm1nd	74	38	reporter	reporter	NN
cord-048471-7jszm1nd	74	39	(	(	-LRB-
cord-048471-7jszm1nd	74	40	Figure	Figure	NNP
cord-048471-7jszm1nd	74	41	2f	2f	NNP
cord-048471-7jszm1nd	74	42	)	)	-RRB-
cord-048471-7jszm1nd	74	43	.	.	.
cord-048471-7jszm1nd	75	1	However	however	RB
cord-048471-7jszm1nd	75	2	,	,	,
cord-048471-7jszm1nd	75	3	no	no	DT
cord-048471-7jszm1nd	75	4	b	b	NN
cord-048471-7jszm1nd	75	5	-	-	HYPH
cord-048471-7jszm1nd	75	6	galactosidase	galactosidase	NN
cord-048471-7jszm1nd	75	7	activity	activity	NN
cord-048471-7jszm1nd	75	8	was	be	VBD
cord-048471-7jszm1nd	75	9	detected	detect	VBN
cord-048471-7jszm1nd	75	10	from	from	IN
cord-048471-7jszm1nd	75	11	cells	cell	NNS
cord-048471-7jszm1nd	75	12	transfected	transfecte	VBN
cord-048471-7jszm1nd	75	13	with	with	IN
cord-048471-7jszm1nd	75	14	the	the	DT
cord-048471-7jszm1nd	75	15	pEGFP	pEGFP	NNP
cord-048471-7jszm1nd	75	16	-	-	HYPH
cord-048471-7jszm1nd	75	17	C1	C1	NNP
cord-048471-7jszm1nd	75	18	/	/	SYM
cord-048471-7jszm1nd	75	19	JV59GS	JV59GS	NNP
cord-048471-7jszm1nd	75	20	/	/	SYM
cord-048471-7jszm1nd	75	21	lacZ	lacZ	-LRB-
cord-048471-7jszm1nd	75	22	construct	construct	VB
cord-048471-7jszm1nd	75	23	(	(	-LRB-
cord-048471-7jszm1nd	75	24	Figure	figure	NN
cord-048471-7jszm1nd	75	25	2b	2b	CD
cord-048471-7jszm1nd	75	26	)	)	-RRB-
cord-048471-7jszm1nd	75	27	,	,	,
cord-048471-7jszm1nd	75	28	demonstrating	demonstrate	VBG
cord-048471-7jszm1nd	75	29	that	that	IN
cord-048471-7jszm1nd	75	30	the	the	DT
cord-048471-7jszm1nd	75	31	JV	JV	NNP
cord-048471-7jszm1nd	75	32	59GS	59gs	CD
cord-048471-7jszm1nd	75	33	was	be	VBD
cord-048471-7jszm1nd	75	34	unable	unable	JJ
cord-048471-7jszm1nd	75	35	to	to	TO
cord-048471-7jszm1nd	75	36	initiate	initiate	VB
cord-048471-7jszm1nd	75	37	translation	translation	NN
cord-048471-7jszm1nd	75	38	,	,	,
cord-048471-7jszm1nd	75	39	and	and	CC
cord-048471-7jszm1nd	75	40	therefore	therefore	RB
cord-048471-7jszm1nd	75	41	,	,	,
cord-048471-7jszm1nd	75	42	in	in	IN
cord-048471-7jszm1nd	75	43	this	this	DT
cord-048471-7jszm1nd	75	44	context	context	NN
cord-048471-7jszm1nd	75	45	,	,	,
cord-048471-7jszm1nd	75	46	did	do	VBD
cord-048471-7jszm1nd	75	47	not	not	RB
cord-048471-7jszm1nd	75	48	possess	possess	VB
cord-048471-7jszm1nd	75	49	any	any	DT
cord-048471-7jszm1nd	75	50	IRES	ires	JJ
cord-048471-7jszm1nd	75	51	-	-	HYPH
cord-048471-7jszm1nd	75	52	like	like	JJ
cord-048471-7jszm1nd	75	53	functions	function	NNS
cord-048471-7jszm1nd	75	54	.	.	.
cord-048471-7jszm1nd	76	1	As	as	IN
cord-048471-7jszm1nd	76	2	it	-PRON-	PRP
cord-048471-7jszm1nd	76	3	was	be	VBD
cord-048471-7jszm1nd	76	4	clear	clear	JJ
cord-048471-7jszm1nd	76	5	that	that	IN
cord-048471-7jszm1nd	76	6	the	the	DT
cord-048471-7jszm1nd	76	7	JV	JV	NNP
cord-048471-7jszm1nd	76	8	59GS	59gs	CD
cord-048471-7jszm1nd	76	9	did	do	VBD
cord-048471-7jszm1nd	76	10	not	not	RB
cord-048471-7jszm1nd	76	11	posses	posse	VBZ
cord-048471-7jszm1nd	76	12	any	any	DT
cord-048471-7jszm1nd	76	13	IRES	IRES	NNP
cord-048471-7jszm1nd	76	14	-	-	HYPH
cord-048471-7jszm1nd	76	15	like	like	JJ
cord-048471-7jszm1nd	76	16	functions	function	NNS
cord-048471-7jszm1nd	76	17	it	-PRON-	PRP
cord-048471-7jszm1nd	76	18	was	be	VBD
cord-048471-7jszm1nd	76	19	necessary	necessary	JJ
cord-048471-7jszm1nd	76	20	to	to	TO
cord-048471-7jszm1nd	76	21	determine	determine	VB
cord-048471-7jszm1nd	76	22	the	the	DT
cord-048471-7jszm1nd	76	23	location	location	NN
cord-048471-7jszm1nd	76	24	of	of	IN
cord-048471-7jszm1nd	76	25	translation	translation	NN
cord-048471-7jszm1nd	76	26	initiation	initiation	NN
cord-048471-7jszm1nd	76	27	within	within	IN
cord-048471-7jszm1nd	76	28	ORF1	ORF1	NNP
cord-048471-7jszm1nd	76	29	.	.	.
cord-048471-7jszm1nd	77	1	This	this	DT
cord-048471-7jszm1nd	77	2	was	be	VBD
cord-048471-7jszm1nd	77	3	predicted	predict	VBN
cord-048471-7jszm1nd	77	4	be	be	VB
cord-048471-7jszm1nd	77	5	the	the	DT
cord-048471-7jszm1nd	77	6	ATG	ATG	NNP
cord-048471-7jszm1nd	77	7	encoding	encode	VBG
cord-048471-7jszm1nd	77	8	methionine	methionine	NN
cord-048471-7jszm1nd	77	9	at	at	IN
cord-048471-7jszm1nd	77	10	nucleotide	nucleotide	JJ
cord-048471-7jszm1nd	77	11	position	position	NN
cord-048471-7jszm1nd	77	12	22	22	CD
cord-048471-7jszm1nd	77	13	,	,	,
cord-048471-7jszm1nd	77	14	as	as	IN
cord-048471-7jszm1nd	77	15	it	-PRON-	PRP
cord-048471-7jszm1nd	77	16	is	be	VBZ
cord-048471-7jszm1nd	77	17	situated	situate	VBN
cord-048471-7jszm1nd	77	18	in	in	IN
cord-048471-7jszm1nd	77	19	a	a	DT
cord-048471-7jszm1nd	77	20	favourable	favourable	JJ
cord-048471-7jszm1nd	77	21	context	context	NN
cord-048471-7jszm1nd	77	22	for	for	IN
cord-048471-7jszm1nd	77	23	translation	translation	NN
cord-048471-7jszm1nd	77	24	initiation	initiation	NN
cord-048471-7jszm1nd	77	25	[	[	-LRB-
cord-048471-7jszm1nd	77	26	7	7	CD
cord-048471-7jszm1nd	77	27	]	]	-RRB-
cord-048471-7jszm1nd	77	28	.	.	.
cord-048471-7jszm1nd	78	1	To	to	TO
cord-048471-7jszm1nd	78	2	investigate	investigate	VB
cord-048471-7jszm1nd	78	3	this	this	DT
cord-048471-7jszm1nd	78	4	multiple	multiple	JJ
cord-048471-7jszm1nd	78	5	translation	translation	NN
cord-048471-7jszm1nd	78	6	termination	termination	NN
cord-048471-7jszm1nd	78	7	codons	codon	NNS
cord-048471-7jszm1nd	78	8	(	(	-LRB-
cord-048471-7jszm1nd	78	9	polySTOP	polySTOP	NNP
cord-048471-7jszm1nd	78	10	)	)	-RRB-
cord-048471-7jszm1nd	78	11	were	be	VBD
cord-048471-7jszm1nd	78	12	inserted	insert	VBN
cord-048471-7jszm1nd	78	13	into	into	IN
cord-048471-7jszm1nd	78	14	the	the	DT
cord-048471-7jszm1nd	78	15	JV	JV	NNP
cord-048471-7jszm1nd	78	16	genome	genome	NN
cord-048471-7jszm1nd	78	17	within	within	IN
cord-048471-7jszm1nd	78	18	the	the	DT
cord-048471-7jszm1nd	78	19	3B	3b	NN
cord-048471-7jszm1nd	78	20	-	-	HYPH
cord-048471-7jszm1nd	78	21	encoding	encode	VBG
cord-048471-7jszm1nd	78	22	region	region	NN
cord-048471-7jszm1nd	78	23	,	,	,
cord-048471-7jszm1nd	78	24	downstream	downstream	RB
cord-048471-7jszm1nd	78	25	of	of	IN
cord-048471-7jszm1nd	78	26	the	the	DT
cord-048471-7jszm1nd	78	27	59GS	59gs	CD
cord-048471-7jszm1nd	78	28	,	,	,
cord-048471-7jszm1nd	78	29	to	to	TO
cord-048471-7jszm1nd	78	30	halt	halt	VB
cord-048471-7jszm1nd	78	31	translation	translation	NN
cord-048471-7jszm1nd	78	32	at	at	IN
cord-048471-7jszm1nd	78	33	a	a	DT
cord-048471-7jszm1nd	78	34	defined	define	VBN
cord-048471-7jszm1nd	78	35	point	point	NN
cord-048471-7jszm1nd	78	36	.	.	.
cord-048471-7jszm1nd	79	1	In	in	IN
cord-048471-7jszm1nd	79	2	vitro	vitro	FW
cord-048471-7jszm1nd	79	3	transcription	transcription	NN
cord-048471-7jszm1nd	79	4	and	and	CC
cord-048471-7jszm1nd	79	5	translation	translation	NN
cord-048471-7jszm1nd	79	6	of	of	IN
cord-048471-7jszm1nd	79	7	this	this	DT
cord-048471-7jszm1nd	79	8	construct	construct	NN
cord-048471-7jszm1nd	79	9	would	would	MD
cord-048471-7jszm1nd	79	10	,	,	,
cord-048471-7jszm1nd	79	11	in	in	IN
cord-048471-7jszm1nd	79	12	theory	theory	NN
cord-048471-7jszm1nd	79	13	,	,	,
cord-048471-7jszm1nd	79	14	yield	yield	VB
cord-048471-7jszm1nd	79	15	a	a	DT
cord-048471-7jszm1nd	79	16	product	product	NN
cord-048471-7jszm1nd	79	17	whose	whose	WP$
cord-048471-7jszm1nd	79	18	size	size	NN
cord-048471-7jszm1nd	79	19	would	would	MD
cord-048471-7jszm1nd	79	20	relate	relate	VB
cord-048471-7jszm1nd	79	21	to	to	IN
cord-048471-7jszm1nd	79	22	the	the	DT
cord-048471-7jszm1nd	79	23	initiation	initiation	NN
cord-048471-7jszm1nd	79	24	codon	codon	NN
cord-048471-7jszm1nd	79	25	used	use	VBN
cord-048471-7jszm1nd	79	26	within	within	IN
cord-048471-7jszm1nd	79	27	the	the	DT
cord-048471-7jszm1nd	79	28	59GS	59gs	CD
cord-048471-7jszm1nd	79	29	(	(	-LRB-
cord-048471-7jszm1nd	79	30	Figure	figure	NN
cord-048471-7jszm1nd	79	31	3	3	CD
cord-048471-7jszm1nd	79	32	)	)	-RRB-
cord-048471-7jszm1nd	79	33	.	.	.
cord-048471-7jszm1nd	80	1	To	to	TO
cord-048471-7jszm1nd	80	2	address	address	VB
cord-048471-7jszm1nd	80	3	the	the	DT
cord-048471-7jszm1nd	80	4	unlikely	unlikely	JJ
cord-048471-7jszm1nd	80	5	event	event	NN
cord-048471-7jszm1nd	80	6	of	of	IN
cord-048471-7jszm1nd	80	7	translation	translation	NN
cord-048471-7jszm1nd	80	8	read	read	VBN
cord-048471-7jszm1nd	80	9	-	-	HYPH
cord-048471-7jszm1nd	80	10	through	through	NN
cord-048471-7jszm1nd	80	11	or	or	CC
cord-048471-7jszm1nd	80	12	re	re	NN
cord-048471-7jszm1nd	80	13	-	-	NN
cord-048471-7jszm1nd	80	14	initiation	initiation	NN
cord-048471-7jszm1nd	80	15	downstream	downstream	RB
cord-048471-7jszm1nd	80	16	of	of	IN
cord-048471-7jszm1nd	80	17	the	the	DT
cord-048471-7jszm1nd	80	18	polySTOP	polystop	NN
cord-048471-7jszm1nd	80	19	,	,	,
cord-048471-7jszm1nd	80	20	which	which	WDT
cord-048471-7jszm1nd	80	21	would	would	MD
cord-048471-7jszm1nd	80	22	result	result	VB
cord-048471-7jszm1nd	80	23	in	in	IN
cord-048471-7jszm1nd	80	24	subsequent	subsequent	JJ
cord-048471-7jszm1nd	80	25	translation	translation	NN
cord-048471-7jszm1nd	80	26	of	of	IN
cord-048471-7jszm1nd	80	27	the	the	DT
cord-048471-7jszm1nd	80	28	3C	3C	NNP
cord-048471-7jszm1nd	80	29	protease	protease	NN
cord-048471-7jszm1nd	80	30	and	and	CC
cord-048471-7jszm1nd	80	31	cleavage	cleavage	NN
cord-048471-7jszm1nd	80	32	of	of	IN
cord-048471-7jszm1nd	80	33	the	the	DT
cord-048471-7jszm1nd	80	34	truncated	truncated	JJ
cord-048471-7jszm1nd	80	35	ORF1	ORF1	NNP
cord-048471-7jszm1nd	80	36	polyprotein	polyprotein	NN
cord-048471-7jszm1nd	80	37	,	,	,
cord-048471-7jszm1nd	80	38	a	a	DT
cord-048471-7jszm1nd	80	39	mutation	mutation	NN
cord-048471-7jszm1nd	80	40	was	be	VBD
cord-048471-7jszm1nd	80	41	made	make	VBN
cord-048471-7jszm1nd	80	42	within	within	IN
cord-048471-7jszm1nd	80	43	the	the	DT
cord-048471-7jszm1nd	80	44	active	active	JJ
cord-048471-7jszm1nd	80	45	site	site	NN
cord-048471-7jszm1nd	80	46	encoding	encode	VBG
cord-048471-7jszm1nd	80	47	region	region	NN
cord-048471-7jszm1nd	80	48	of	of	IN
cord-048471-7jszm1nd	80	49	the	the	DT
cord-048471-7jszm1nd	80	50	3C	3C	NNP
cord-048471-7jszm1nd	80	51	protease	protease	NN
cord-048471-7jszm1nd	80	52	within	within	IN
cord-048471-7jszm1nd	80	53	JV	JV	NNP
cord-048471-7jszm1nd	80	54	ORF1	ORF1	NNP
cord-048471-7jszm1nd	80	55	,	,	,
cord-048471-7jszm1nd	80	56	to	to	TO
cord-048471-7jszm1nd	80	57	prevent	prevent	VB
cord-048471-7jszm1nd	80	58	any	any	DT
cord-048471-7jszm1nd	80	59	viral	viral	JJ
cord-048471-7jszm1nd	80	60	mediated	mediate	VBN
cord-048471-7jszm1nd	80	61	cleavage	cleavage	NN
cord-048471-7jszm1nd	80	62	of	of	IN
cord-048471-7jszm1nd	80	63	ORF1	ORF1	NNP
cord-048471-7jszm1nd	80	64	translation	translation	NN
cord-048471-7jszm1nd	80	65	products	product	NNS
cord-048471-7jszm1nd	80	66	(	(	-LRB-
cord-048471-7jszm1nd	80	67	JV	JV	NNP
cord-048471-7jszm1nd	80	68	3C	3C	NNP
cord-048471-7jszm1nd	80	69	mut	mut	NNP
cord-048471-7jszm1nd	80	70	/polySTOP	/polySTOP	NNP
cord-048471-7jszm1nd	80	71	)	)	-RRB-
cord-048471-7jszm1nd	80	72	.	.	.
cord-048471-7jszm1nd	81	1	A	a	DT
cord-048471-7jszm1nd	81	2	point	point	NN
cord-048471-7jszm1nd	81	3	mutation	mutation	NN
cord-048471-7jszm1nd	81	4	of	of	IN
cord-048471-7jszm1nd	81	5	the	the	DT
cord-048471-7jszm1nd	81	6	critical	critical	JJ
cord-048471-7jszm1nd	81	7	cysteine	cysteine	NN
cord-048471-7jszm1nd	81	8	residue	residue	NN
cord-048471-7jszm1nd	81	9	within	within	IN
cord-048471-7jszm1nd	81	10	the	the	DT
cord-048471-7jszm1nd	81	11	highly	highly	RB
cord-048471-7jszm1nd	81	12	conserved	conserve	VBN
cord-048471-7jszm1nd	81	13	GDCG	GDCG	NNP
cord-048471-7jszm1nd	81	14	motif	motif	NN
cord-048471-7jszm1nd	81	15	to	to	IN
cord-048471-7jszm1nd	81	16	a	a	DT
cord-048471-7jszm1nd	81	17	glycine	glycine	NN
cord-048471-7jszm1nd	81	18	residue	residue	NN
cord-048471-7jszm1nd	81	19	was	be	VBD
cord-048471-7jszm1nd	81	20	performed	perform	VBN
cord-048471-7jszm1nd	81	21	,	,	,
cord-048471-7jszm1nd	81	22	and	and	CC
cord-048471-7jszm1nd	81	23	this	this	DT
cord-048471-7jszm1nd	81	24	approach	approach	NN
cord-048471-7jszm1nd	81	25	has	have	VBZ
cord-048471-7jszm1nd	81	26	been	be	VBN
cord-048471-7jszm1nd	81	27	described	describe	VBN
cord-048471-7jszm1nd	81	28	for	for	IN
cord-048471-7jszm1nd	81	29	the	the	DT
cord-048471-7jszm1nd	81	30	successful	successful	JJ
cord-048471-7jszm1nd	81	31	inactivation	inactivation	NN
cord-048471-7jszm1nd	81	32	of	of	IN
cord-048471-7jszm1nd	81	33	other	other	JJ
cord-048471-7jszm1nd	81	34	norovirus	norovirus	NN
cord-048471-7jszm1nd	81	35	'	'	POS
cord-048471-7jszm1nd	81	36	3C	3c	NN
cord-048471-7jszm1nd	81	37	activity	activity	NN
cord-048471-7jszm1nd	81	38	[	[	-LRB-
cord-048471-7jszm1nd	81	39	28	28	CD
cord-048471-7jszm1nd	81	40	]	]	-RRB-
cord-048471-7jszm1nd	81	41	.	.	.
cord-048471-7jszm1nd	82	1	In	in	IN
cord-048471-7jszm1nd	82	2	vitro	vitro	FW
cord-048471-7jszm1nd	82	3	transcription	transcription	NN
cord-048471-7jszm1nd	82	4	and	and	CC
cord-048471-7jszm1nd	82	5	translation	translation	NN
cord-048471-7jszm1nd	82	6	analysis	analysis	NN
cord-048471-7jszm1nd	82	7	was	be	VBD
cord-048471-7jszm1nd	82	8	performed	perform	VBN
cord-048471-7jszm1nd	82	9	on	on	IN
cord-048471-7jszm1nd	82	10	JV	JV	NNP
cord-048471-7jszm1nd	82	11	wild	wild	JJ
cord-048471-7jszm1nd	82	12	type	type	NN
cord-048471-7jszm1nd	82	13	(	(	-LRB-
cord-048471-7jszm1nd	82	14	Figure	figure	NN
cord-048471-7jszm1nd	82	15	4	4	CD
cord-048471-7jszm1nd	82	16	within	within	IN
cord-048471-7jszm1nd	82	17	the	the	DT
cord-048471-7jszm1nd	82	18	3C	3C	NNP
cord-048471-7jszm1nd	82	19	region	region	NN
cord-048471-7jszm1nd	82	20	of	of	IN
cord-048471-7jszm1nd	82	21	JV	JV	NNP
cord-048471-7jszm1nd	82	22	successfully	successfully	RB
cord-048471-7jszm1nd	82	23	inactivated	inactivate	VBD
cord-048471-7jszm1nd	82	24	the	the	DT
cord-048471-7jszm1nd	82	25	3C	3C	NNP
cord-048471-7jszm1nd	82	26	protease	protease	NN
cord-048471-7jszm1nd	82	27	,	,	,
cord-048471-7jszm1nd	82	28	thus	thus	RB
cord-048471-7jszm1nd	82	29	a	a	DT
cord-048471-7jszm1nd	82	30	large	large	JJ
cord-048471-7jszm1nd	82	31	,	,	,
cord-048471-7jszm1nd	82	32	.200kDa	.200kDa	NFP
cord-048471-7jszm1nd	82	33	uncleaved	uncleaved	NNP
cord-048471-7jszm1nd	82	34	polyprotein	polyprotein	NNP
cord-048471-7jszm1nd	82	35	is	be	VBZ
cord-048471-7jszm1nd	82	36	yielded	yield	VBN
cord-048471-7jszm1nd	82	37	following	follow	VBG
cord-048471-7jszm1nd	82	38	TNTH	TNTH	NNS
cord-048471-7jszm1nd	82	39	.	.	.
cord-048471-7jszm1nd	83	1	The	the	DT
cord-048471-7jszm1nd	83	2	major	major	JJ
cord-048471-7jszm1nd	83	3	product	product	NN
cord-048471-7jszm1nd	83	4	generated	generate	VBN
cord-048471-7jszm1nd	83	5	by	by	IN
cord-048471-7jszm1nd	83	6	JV	JV	NNP
cord-048471-7jszm1nd	83	7	3C	3C	NNP
cord-048471-7jszm1nd	83	8	mut	mut	NNP
cord-048471-7jszm1nd	83	9	/	/	SYM
cord-048471-7jszm1nd	83	10	polySTOP	polySTOP	NNP
cord-048471-7jszm1nd	83	11	was	be	VBD
cord-048471-7jszm1nd	83	12	calculated	calculate	VBN
cord-048471-7jszm1nd	83	13	to	to	TO
cord-048471-7jszm1nd	83	14	be	be	VB
cord-048471-7jszm1nd	83	15	103kDa	103kDa	VBN
cord-048471-7jszm1nd	83	16	in	in	IN
cord-048471-7jszm1nd	83	17	size	size	NN
cord-048471-7jszm1nd	83	18	.	.	.
cord-048471-7jszm1nd	84	1	Based	base	VBN
cord-048471-7jszm1nd	84	2	on	on	IN
cord-048471-7jszm1nd	84	3	computer	computer	NN
cord-048471-7jszm1nd	84	4	predictions	prediction	NNS
cord-048471-7jszm1nd	84	5	this	this	DT
cord-048471-7jszm1nd	84	6	is	be	VBZ
cord-048471-7jszm1nd	84	7	in	in	IN
cord-048471-7jszm1nd	84	8	agreement	agreement	NN
cord-048471-7jszm1nd	84	9	with	with	IN
cord-048471-7jszm1nd	84	10	the	the	DT
cord-048471-7jszm1nd	84	11	initiation	initiation	NN
cord-048471-7jszm1nd	84	12	of	of	IN
cord-048471-7jszm1nd	84	13	translation	translation	NN
cord-048471-7jszm1nd	84	14	occurring	occur	VBG
cord-048471-7jszm1nd	84	15	at	at	IN
cord-048471-7jszm1nd	84	16	nucleotide	nucleotide	JJ
cord-048471-7jszm1nd	84	17	22	22	CD
cord-048471-7jszm1nd	84	18	,	,	,
cord-048471-7jszm1nd	84	19	which	which	WDT
cord-048471-7jszm1nd	84	20	demonstrates	demonstrate	VBZ
cord-048471-7jszm1nd	84	21	that	that	IN
cord-048471-7jszm1nd	84	22	the	the	DT
cord-048471-7jszm1nd	84	23	JV	JV	NNP
cord-048471-7jszm1nd	84	24	N	N	NNP
cord-048471-7jszm1nd	84	25	-	-	HYPH
cord-048471-7jszm1nd	84	26	term	term	NN
cord-048471-7jszm1nd	84	27	protein	protein	NN
cord-048471-7jszm1nd	84	28	is	be	VBZ
cord-048471-7jszm1nd	84	29	translated	translate	VBN
cord-048471-7jszm1nd	84	30	in	in	IN
cord-048471-7jszm1nd	84	31	full	full	JJ
cord-048471-7jszm1nd	84	32	in	in	IN
cord-048471-7jszm1nd	84	33	vitro	vitro	FW
cord-048471-7jszm1nd	84	34	.	.	.
cord-048471-7jszm1nd	85	1	At	at	IN
cord-048471-7jszm1nd	85	2	this	this	DT
cord-048471-7jszm1nd	85	3	time	time	NN
cord-048471-7jszm1nd	85	4	it	-PRON-	PRP
cord-048471-7jszm1nd	85	5	is	be	VBZ
cord-048471-7jszm1nd	85	6	not	not	RB
cord-048471-7jszm1nd	85	7	possible	possible	JJ
cord-048471-7jszm1nd	85	8	to	to	TO
cord-048471-7jszm1nd	85	9	determine	determine	VB
cord-048471-7jszm1nd	85	10	whether	whether	IN
cord-048471-7jszm1nd	85	11	translation	translation	NN
cord-048471-7jszm1nd	85	12	of	of	IN
cord-048471-7jszm1nd	85	13	intracellular	intracellular	JJ
cord-048471-7jszm1nd	85	14	VPgbound	vpgbound	JJ
cord-048471-7jszm1nd	85	15	viral	viral	JJ
cord-048471-7jszm1nd	85	16	RNA	RNA	NNP
cord-048471-7jszm1nd	85	17	initiates	initiate	NNS
cord-048471-7jszm1nd	85	18	at	at	IN
cord-048471-7jszm1nd	85	19	nucleotide	nucleotide	JJ
cord-048471-7jszm1nd	85	20	22	22	CD
cord-048471-7jszm1nd	85	21	,	,	,
cord-048471-7jszm1nd	85	22	although	although	IN
cord-048471-7jszm1nd	85	23	it	-PRON-	PRP
cord-048471-7jszm1nd	85	24	is	be	VBZ
cord-048471-7jszm1nd	85	25	likely	likely	RB
cord-048471-7jszm1nd	85	26	given	give	VBN
cord-048471-7jszm1nd	85	27	the	the	DT
cord-048471-7jszm1nd	85	28	favourable	favourable	JJ
cord-048471-7jszm1nd	85	29	context	context	NN
cord-048471-7jszm1nd	85	30	in	in	IN
cord-048471-7jszm1nd	85	31	which	which	WDT
cord-048471-7jszm1nd	85	32	the	the	DT
cord-048471-7jszm1nd	85	33	initiation	initiation	NN
cord-048471-7jszm1nd	85	34	codon	codon	NN
cord-048471-7jszm1nd	85	35	is	be	VBZ
cord-048471-7jszm1nd	85	36	situated	situate	VBN
cord-048471-7jszm1nd	85	37	.	.	.
cord-048471-7jszm1nd	86	1	As	as	IN
cord-048471-7jszm1nd	86	2	the	the	DT
cord-048471-7jszm1nd	86	3	JV	JV	NNP
cord-048471-7jszm1nd	86	4	N	N	NNP
cord-048471-7jszm1nd	86	5	-	-	HYPH
cord-048471-7jszm1nd	86	6	term	term	NN
cord-048471-7jszm1nd	86	7	was	be	VBD
cord-048471-7jszm1nd	86	8	found	find	VBN
cord-048471-7jszm1nd	86	9	to	to	TO
cord-048471-7jszm1nd	86	10	be	be	VB
cord-048471-7jszm1nd	86	11	translated	translate	VBN
cord-048471-7jszm1nd	86	12	in	in	IN
cord-048471-7jszm1nd	86	13	vitro	vitro	FW
cord-048471-7jszm1nd	86	14	attempts	attempt	NNS
cord-048471-7jszm1nd	86	15	were	be	VBD
cord-048471-7jszm1nd	86	16	made	make	VBN
cord-048471-7jszm1nd	86	17	to	to	TO
cord-048471-7jszm1nd	86	18	express	express	VB
cord-048471-7jszm1nd	86	19	and	and	CC
cord-048471-7jszm1nd	86	20	purify	purify	VB
cord-048471-7jszm1nd	86	21	the	the	DT
cord-048471-7jszm1nd	86	22	protein	protein	NN
cord-048471-7jszm1nd	86	23	in	in	IN
cord-048471-7jszm1nd	86	24	bacteria	bacteria	NNS
cord-048471-7jszm1nd	86	25	for	for	IN
cord-048471-7jszm1nd	86	26	immunisation	immunisation	NN
cord-048471-7jszm1nd	86	27	so	so	IN
cord-048471-7jszm1nd	86	28	that	that	IN
cord-048471-7jszm1nd	86	29	the	the	DT
cord-048471-7jszm1nd	86	30	protein	protein	NN
cord-048471-7jszm1nd	86	31	could	could	MD
cord-048471-7jszm1nd	86	32	be	be	VB
cord-048471-7jszm1nd	86	33	identified	identify	VBN
cord-048471-7jszm1nd	86	34	by	by	IN
cord-048471-7jszm1nd	86	35	radioimmune	radioimmune	JJ
cord-048471-7jszm1nd	86	36	precipitation	precipitation	NN
cord-048471-7jszm1nd	86	37	assay	assay	NN
cord-048471-7jszm1nd	86	38	(	(	-LRB-
cord-048471-7jszm1nd	86	39	RIPA	RIPA	NNP
cord-048471-7jszm1nd	86	40	)	)	-RRB-
cord-048471-7jszm1nd	86	41	,	,	,
cord-048471-7jszm1nd	86	42	as	as	IN
cord-048471-7jszm1nd	86	43	it	-PRON-	PRP
cord-048471-7jszm1nd	86	44	was	be	VBD
cord-048471-7jszm1nd	86	45	possible	possible	JJ
cord-048471-7jszm1nd	86	46	that	that	IN
cord-048471-7jszm1nd	86	47	the	the	DT
cord-048471-7jszm1nd	86	48	Nterm	Nterm	NNP
cord-048471-7jszm1nd	86	49	protein	protein	NN
cord-048471-7jszm1nd	86	50	was	be	VBD
cord-048471-7jszm1nd	86	51	migrating	migrate	VBG
cord-048471-7jszm1nd	86	52	on	on	IN
cord-048471-7jszm1nd	86	53	gels	gel	NNS
cord-048471-7jszm1nd	86	54	aberrantly	aberrantly	RB
cord-048471-7jszm1nd	86	55	and	and	CC
cord-048471-7jszm1nd	86	56	possibly	possibly	RB
cord-048471-7jszm1nd	86	57	comigrating	comigrate	VBG
cord-048471-7jszm1nd	86	58	with	with	IN
cord-048471-7jszm1nd	86	59	2C.	2c.	CD
cord-048471-7jszm1nd	86	60	Attempts	attempt	NNS
cord-048471-7jszm1nd	86	61	to	to	TO
cord-048471-7jszm1nd	86	62	express	express	VB
cord-048471-7jszm1nd	86	63	the	the	DT
cord-048471-7jszm1nd	86	64	protein	protein	NN
cord-048471-7jszm1nd	86	65	in	in	IN
cord-048471-7jszm1nd	86	66	bacteria	bacteria	NNS
cord-048471-7jszm1nd	86	67	were	be	VBD
cord-048471-7jszm1nd	86	68	unsuccessful	unsuccessful	JJ
cord-048471-7jszm1nd	86	69	due	due	IN
cord-048471-7jszm1nd	86	70	to	to	IN
cord-048471-7jszm1nd	86	71	toxicity	toxicity	NN
cord-048471-7jszm1nd	86	72	.	.	.
cord-048471-7jszm1nd	87	1	Therefore	therefore	RB
cord-048471-7jszm1nd	87	2	,	,	,
cord-048471-7jszm1nd	87	3	the	the	DT
cord-048471-7jszm1nd	87	4	14aa	14aa	JJ
cord-048471-7jszm1nd	87	5	V5	v5	NN
cord-048471-7jszm1nd	87	6	epitope	epitope	NN
cord-048471-7jszm1nd	87	7	encoding	encode	VBG
cord-048471-7jszm1nd	87	8	sequence	sequence	NN
cord-048471-7jszm1nd	87	9	was	be	VBD
cord-048471-7jszm1nd	87	10	cloned	clone	VBN
cord-048471-7jszm1nd	87	11	in	in	IN
cord-048471-7jszm1nd	87	12	frame	frame	NN
cord-048471-7jszm1nd	87	13	into	into	IN
cord-048471-7jszm1nd	87	14	the	the	DT
cord-048471-7jszm1nd	87	15	JV	JV	NNP
cord-048471-7jszm1nd	87	16	cDNA	cdna	NN
cord-048471-7jszm1nd	87	17	construct	construct	NN
cord-048471-7jszm1nd	87	18	at	at	IN
cord-048471-7jszm1nd	87	19	nucleotide	nucleotide	JJ
cord-048471-7jszm1nd	87	20	position	position	NN
cord-048471-7jszm1nd	87	21	123	123	CD
cord-048471-7jszm1nd	87	22	(	(	-LRB-
cord-048471-7jszm1nd	87	23	JV	JV	NNP
cord-048471-7jszm1nd	87	24	V5	V5	NNP
cord-048471-7jszm1nd	87	25	)	)	-RRB-
cord-048471-7jszm1nd	87	26	.	.	.
cord-048471-7jszm1nd	88	1	The	the	DT
cord-048471-7jszm1nd	88	2	V5	V5	NNP
cord-048471-7jszm1nd	88	3	epitope	epitope	NN
cord-048471-7jszm1nd	88	4	originates	originate	VBZ
cord-048471-7jszm1nd	88	5	from	from	IN
cord-048471-7jszm1nd	88	6	the	the	DT
cord-048471-7jszm1nd	88	7	P	p	NN
cord-048471-7jszm1nd	88	8	and	and	CC
cord-048471-7jszm1nd	88	9	V	v	NN
cord-048471-7jszm1nd	88	10	proteins	protein	NNS
cord-048471-7jszm1nd	88	11	of	of	IN
cord-048471-7jszm1nd	88	12	the	the	DT
cord-048471-7jszm1nd	88	13	SV5	SV5	NNP
cord-048471-7jszm1nd	88	14	paramyxovirus	paramyxovirus	NNP
cord-048471-7jszm1nd	88	15	[	[	-LRB-
cord-048471-7jszm1nd	88	16	29	29	CD
cord-048471-7jszm1nd	88	17	]	]	-RRB-
cord-048471-7jszm1nd	88	18	,	,	,
cord-048471-7jszm1nd	88	19	for	for	IN
cord-048471-7jszm1nd	88	20	which	which	WDT
cord-048471-7jszm1nd	88	21	a	a	DT
cord-048471-7jszm1nd	88	22	commercially	commercially	RB
cord-048471-7jszm1nd	88	23	available	available	JJ
cord-048471-7jszm1nd	88	24	monoclonal	monoclonal	JJ
cord-048471-7jszm1nd	88	25	antibody	antibody	NN
cord-048471-7jszm1nd	88	26	is	be	VBZ
cord-048471-7jszm1nd	88	27	used	use	VBN
cord-048471-7jszm1nd	88	28	for	for	IN
cord-048471-7jszm1nd	88	29	detection	detection	NN
cord-048471-7jszm1nd	88	30	.	.	.
cord-048471-7jszm1nd	89	1	Following	follow	VBG
cord-048471-7jszm1nd	89	2	in	in	IN
cord-048471-7jszm1nd	89	3	vitro	vitro	FW
cord-048471-7jszm1nd	89	4	transcription	transcription	NN
cord-048471-7jszm1nd	89	5	and	and	CC
cord-048471-7jszm1nd	89	6	translation	translation	NN
cord-048471-7jszm1nd	89	7	of	of	IN
cord-048471-7jszm1nd	89	8	JV	JV	NNP
cord-048471-7jszm1nd	89	9	V5	V5	NNP
cord-048471-7jszm1nd	89	10	a	a	DT
cord-048471-7jszm1nd	89	11	new	new	JJ
cord-048471-7jszm1nd	89	12	product	product	NN
cord-048471-7jszm1nd	89	13	,	,	,
cord-048471-7jszm1nd	89	14	approximately	approximately	RB
cord-048471-7jszm1nd	89	15	42	42	CD
cord-048471-7jszm1nd	89	16	kDa	kDa	NNS
cord-048471-7jszm1nd	89	17	in	in	IN
cord-048471-7jszm1nd	89	18	size	size	NN
cord-048471-7jszm1nd	89	19	,	,	,
cord-048471-7jszm1nd	89	20	was	be	VBD
cord-048471-7jszm1nd	89	21	visible	visible	JJ
cord-048471-7jszm1nd	89	22	(	(	-LRB-
cord-048471-7jszm1nd	89	23	Figure	figure	NN
cord-048471-7jszm1nd	89	24	5	5	CD
cord-048471-7jszm1nd	89	25	,	,	,
cord-048471-7jszm1nd	89	26	lane	lane	NN
cord-048471-7jszm1nd	89	27	2	2	CD
cord-048471-7jszm1nd	89	28	)	)	-RRB-
cord-048471-7jszm1nd	89	29	.	.	.
cord-048471-7jszm1nd	90	1	This	this	DT
cord-048471-7jszm1nd	90	2	product	product	NN
cord-048471-7jszm1nd	90	3	was	be	VBD
cord-048471-7jszm1nd	90	4	not	not	RB
cord-048471-7jszm1nd	90	5	observed	observe	VBN
cord-048471-7jszm1nd	90	6	in	in	IN
cord-048471-7jszm1nd	90	7	any	any	DT
cord-048471-7jszm1nd	90	8	prior	prior	JJ
cord-048471-7jszm1nd	90	9	analyses	analysis	NNS
cord-048471-7jszm1nd	90	10	of	of	IN
cord-048471-7jszm1nd	90	11	JV	JV	NNP
cord-048471-7jszm1nd	90	12	.	.	.
cord-048471-7jszm1nd	91	1	To	to	TO
cord-048471-7jszm1nd	91	2	confirm	confirm	VB
cord-048471-7jszm1nd	91	3	that	that	IN
cord-048471-7jszm1nd	91	4	this	this	DT
cord-048471-7jszm1nd	91	5	protein	protein	NN
cord-048471-7jszm1nd	91	6	was	be	VBD
cord-048471-7jszm1nd	91	7	V5	V5	NNP
cord-048471-7jszm1nd	91	8	/	/	SYM
cord-048471-7jszm1nd	91	9	N	n	CD
cord-048471-7jszm1nd	91	10	-	-	HYPH
cord-048471-7jszm1nd	91	11	term	term	NN
cord-048471-7jszm1nd	91	12	associated	associate	VBN
cord-048471-7jszm1nd	92	1	the	the	DT
cord-048471-7jszm1nd	92	2	TNTH	TNTH	NNP
cord-048471-7jszm1nd	92	3	reaction	reaction	NN
cord-048471-7jszm1nd	92	4	was	be	VBD
cord-048471-7jszm1nd	92	5	subjected	subject	VBN
cord-048471-7jszm1nd	92	6	to	to	IN
cord-048471-7jszm1nd	92	7	RIPA	RIPA	NNP
cord-048471-7jszm1nd	92	8	using	use	VBG
cord-048471-7jszm1nd	92	9	the	the	DT
cord-048471-7jszm1nd	92	10	anti	anti	JJ
cord-048471-7jszm1nd	92	11	-	-	JJ
cord-048471-7jszm1nd	92	12	V5	v5	JJ
cord-048471-7jszm1nd	92	13	antibody	antibody	NN
cord-048471-7jszm1nd	92	14	(	(	-LRB-
cord-048471-7jszm1nd	92	15	Figure	Figure	NNP
cord-048471-7jszm1nd	92	16	5	5	CD
cord-048471-7jszm1nd	92	17	,	,	,
cord-048471-7jszm1nd	92	18	lane	lane	NN
cord-048471-7jszm1nd	92	19	3	3	CD
cord-048471-7jszm1nd	92	20	)	)	-RRB-
cord-048471-7jszm1nd	92	21	.	.	.
cord-048471-7jszm1nd	93	1	This	this	DT
cord-048471-7jszm1nd	93	2	confirmed	confirm	VBD
cord-048471-7jszm1nd	93	3	expression	expression	NN
cord-048471-7jszm1nd	93	4	of	of	IN
cord-048471-7jszm1nd	93	5	the	the	DT
cord-048471-7jszm1nd	93	6	N	n	CD
cord-048471-7jszm1nd	93	7	-	-	HYPH
cord-048471-7jszm1nd	93	8	term	term	NN
cord-048471-7jszm1nd	93	9	protein	protein	NN
cord-048471-7jszm1nd	93	10	in	in	IN
cord-048471-7jszm1nd	93	11	vitro	vitro	FW
cord-048471-7jszm1nd	93	12	.	.	.
cord-048471-7jszm1nd	94	1	To	to	TO
cord-048471-7jszm1nd	94	2	confirm	confirm	VB
cord-048471-7jszm1nd	94	3	expression	expression	NN
cord-048471-7jszm1nd	94	4	of	of	IN
cord-048471-7jszm1nd	94	5	the	the	DT
cord-048471-7jszm1nd	94	6	V5	V5	NNP
cord-048471-7jszm1nd	94	7	/	/	SYM
cord-048471-7jszm1nd	94	8	N	n	CD
cord-048471-7jszm1nd	94	9	-	-	HYPH
cord-048471-7jszm1nd	94	10	term	term	NN
cord-048471-7jszm1nd	94	11	protein	protein	NN
cord-048471-7jszm1nd	94	12	in	in	IN
cord-048471-7jszm1nd	94	13	cell	cell	NN
cord-048471-7jszm1nd	94	14	culture	culture	NN
cord-048471-7jszm1nd	94	15	capped	cap	VBN
cord-048471-7jszm1nd	94	16	RNA	RNA	NNP
cord-048471-7jszm1nd	94	17	was	be	VBD
cord-048471-7jszm1nd	94	18	synthesised	synthesise	VBN
cord-048471-7jszm1nd	94	19	from	from	IN
cord-048471-7jszm1nd	94	20	the	the	DT
cord-048471-7jszm1nd	94	21	JV	JV	NNP
cord-048471-7jszm1nd	94	22	V5	V5	NNP
cord-048471-7jszm1nd	94	23	T7	T7	NNP
cord-048471-7jszm1nd	94	24	cDNA	cdna	NN
cord-048471-7jszm1nd	94	25	construct	construct	NN
cord-048471-7jszm1nd	94	26	,	,	,
cord-048471-7jszm1nd	94	27	which	which	WDT
cord-048471-7jszm1nd	94	28	was	be	VBD
cord-048471-7jszm1nd	94	29	used	use	VBN
cord-048471-7jszm1nd	94	30	to	to	TO
cord-048471-7jszm1nd	94	31	transfect	transfect	VB
cord-048471-7jszm1nd	94	32	CRFK	CRFK	NNP
cord-048471-7jszm1nd	94	33	cells	cell	NNS
cord-048471-7jszm1nd	94	34	.	.	.
cord-048471-7jszm1nd	95	1	As	as	IN
cord-048471-7jszm1nd	95	2	there	there	EX
cord-048471-7jszm1nd	95	3	is	be	VBZ
cord-048471-7jszm1nd	95	4	currently	currently	RB
cord-048471-7jszm1nd	95	5	no	no	DT
cord-048471-7jszm1nd	95	6	host	host	NN
cord-048471-7jszm1nd	95	7	cell	cell	NN
cord-048471-7jszm1nd	95	8	line	line	NN
cord-048471-7jszm1nd	95	9	in	in	IN
cord-048471-7jszm1nd	95	10	which	which	WDT
cord-048471-7jszm1nd	95	11	to	to	TO
cord-048471-7jszm1nd	95	12	propagate	propagate	VB
cord-048471-7jszm1nd	95	13	JV	JV	NNP
cord-048471-7jszm1nd	95	14	the	the	DT
cord-048471-7jszm1nd	95	15	CRFK	CRFK	NNP
cord-048471-7jszm1nd	95	16	cell	cell	NN
cord-048471-7jszm1nd	95	17	line	line	NN
cord-048471-7jszm1nd	95	18	was	be	VBD
cord-048471-7jszm1nd	95	19	used	use	VBN
cord-048471-7jszm1nd	95	20	as	as	IN
cord-048471-7jszm1nd	95	21	it	-PRON-	PRP
cord-048471-7jszm1nd	95	22	has	have	VBZ
cord-048471-7jszm1nd	95	23	been	be	VBN
cord-048471-7jszm1nd	95	24	shown	show	VBN
cord-048471-7jszm1nd	95	25	to	to	TO
cord-048471-7jszm1nd	95	26	support	support	VB
cord-048471-7jszm1nd	95	27	the	the	DT
cord-048471-7jszm1nd	95	28	replication	replication	NN
cord-048471-7jszm1nd	95	29	of	of	IN
cord-048471-7jszm1nd	95	30	feline	feline	JJ
cord-048471-7jszm1nd	95	31	calicivirus	calicivirus	NNP
cord-048471-7jszm1nd	95	32	[	[	-LRB-
cord-048471-7jszm1nd	95	33	30	30	CD
cord-048471-7jszm1nd	95	34	]	]	-RRB-
cord-048471-7jszm1nd	95	35	.	.	.
cord-048471-7jszm1nd	96	1	Confocal	confocal	JJ
cord-048471-7jszm1nd	96	2	immunofluorescence	immunofluorescence	NN
cord-048471-7jszm1nd	96	3	of	of	IN
cord-048471-7jszm1nd	96	4	transfected	transfecte	VBN
cord-048471-7jszm1nd	96	5	cells	cell	NNS
cord-048471-7jszm1nd	96	6	using	use	VBG
cord-048471-7jszm1nd	96	7	the	the	DT
cord-048471-7jszm1nd	96	8	anti	anti	JJ
cord-048471-7jszm1nd	96	9	-	-	JJ
cord-048471-7jszm1nd	96	10	V5	v5	JJ
cord-048471-7jszm1nd	96	11	antibody	antibody	NN
cord-048471-7jszm1nd	96	12	demonstrated	demonstrate	VBD
cord-048471-7jszm1nd	96	13	expression	expression	NN
cord-048471-7jszm1nd	96	14	of	of	IN
cord-048471-7jszm1nd	96	15	the	the	DT
cord-048471-7jszm1nd	96	16	V5	V5	NNP
cord-048471-7jszm1nd	96	17	/	/	SYM
cord-048471-7jszm1nd	96	18	Nterm	Nterm	NNP
cord-048471-7jszm1nd	96	19	protein	protein	NN
cord-048471-7jszm1nd	96	20	in	in	IN
cord-048471-7jszm1nd	96	21	cultured	cultured	JJ
cord-048471-7jszm1nd	96	22	cells	cell	NNS
cord-048471-7jszm1nd	96	23	(	(	-LRB-
cord-048471-7jszm1nd	96	24	Figure	figure	NN
cord-048471-7jszm1nd	96	25	6	6	CD
cord-048471-7jszm1nd	96	26	)	)	-RRB-
cord-048471-7jszm1nd	96	27	.	.	.
cord-048471-7jszm1nd	97	1	Expression	expression	NN
cord-048471-7jszm1nd	97	2	of	of	IN
cord-048471-7jszm1nd	97	3	the	the	DT
cord-048471-7jszm1nd	97	4	V5/	v5/	NN
cord-048471-7jszm1nd	98	1	Nterm	Nterm	NNP
cord-048471-7jszm1nd	98	2	protein	protein	NN
cord-048471-7jszm1nd	98	3	was	be	VBD
cord-048471-7jszm1nd	98	4	diffuse	diffuse	JJ
cord-048471-7jszm1nd	98	5	and	and	CC
cord-048471-7jszm1nd	98	6	did	do	VBD
cord-048471-7jszm1nd	98	7	not	not	RB
cord-048471-7jszm1nd	98	8	co	co	VB
cord-048471-7jszm1nd	98	9	-	-	VB
cord-048471-7jszm1nd	98	10	localise	localise	VB
cord-048471-7jszm1nd	98	11	with	with	IN
cord-048471-7jszm1nd	98	12	the	the	DT
cord-048471-7jszm1nd	98	13	Golgi/	Golgi/	NNP
cord-048471-7jszm1nd	98	14	ER	ER	NNP
cord-048471-7jszm1nd	98	15	/	/	SYM
cord-048471-7jszm1nd	98	16	plasma	plasma	NN
cord-048471-7jszm1nd	98	17	membrane	membrane	NN
cord-048471-7jszm1nd	98	18	marker	marker	NN
cord-048471-7jszm1nd	98	19	wheat	wheat	NN
cord-048471-7jszm1nd	98	20	germ	germ	NNP
cord-048471-7jszm1nd	98	21	agglutinin	agglutinin	NNP
cord-048471-7jszm1nd	98	22	(	(	-LRB-
cord-048471-7jszm1nd	98	23	WGA	WGA	NNP
cord-048471-7jszm1nd	98	24	)	)	-RRB-
cord-048471-7jszm1nd	98	25	and	and	CC
cord-048471-7jszm1nd	98	26	therefore	therefore	RB
cord-048471-7jszm1nd	98	27	displays	display	VBZ
cord-048471-7jszm1nd	98	28	a	a	DT
cord-048471-7jszm1nd	98	29	different	different	JJ
cord-048471-7jszm1nd	98	30	pattern	pattern	NN
cord-048471-7jszm1nd	98	31	of	of	IN
cord-048471-7jszm1nd	98	32	cellular	cellular	JJ
cord-048471-7jszm1nd	98	33	expression	expression	NN
cord-048471-7jszm1nd	98	34	compared	compare	VBN
cord-048471-7jszm1nd	98	35	to	to	IN
cord-048471-7jszm1nd	98	36	Norwalk	Norwalk	NNP
cord-048471-7jszm1nd	98	37	virus	virus	NN
cord-048471-7jszm1nd	98	38	[	[	-LRB-
cord-048471-7jszm1nd	98	39	15	15	CD
cord-048471-7jszm1nd	98	40	]	]	-RRB-
cord-048471-7jszm1nd	98	41	.	.	.
cord-048471-7jszm1nd	99	1	Cells	cell	NNS
cord-048471-7jszm1nd	99	2	transfected	transfecte	VBN
cord-048471-7jszm1nd	99	3	with	with	IN
cord-048471-7jszm1nd	99	4	the	the	DT
cord-048471-7jszm1nd	99	5	wild	wild	JJ
cord-048471-7jszm1nd	99	6	type	type	NN
cord-048471-7jszm1nd	99	7	full	full	JJ
cord-048471-7jszm1nd	99	8	length	length	NN
cord-048471-7jszm1nd	99	9	JV	JV	NNP
cord-048471-7jszm1nd	99	10	RNA	RNA	NNP
cord-048471-7jszm1nd	99	11	were	be	VBD
cord-048471-7jszm1nd	99	12	negative	negative	JJ
cord-048471-7jszm1nd	99	13	for	for	IN
cord-048471-7jszm1nd	99	14	fluorescence	fluorescence	NN
cord-048471-7jszm1nd	99	15	(	(	-LRB-
cord-048471-7jszm1nd	99	16	data	datum	NNS
cord-048471-7jszm1nd	99	17	not	not	RB
cord-048471-7jszm1nd	99	18	shown	show	VBN
cord-048471-7jszm1nd	99	19	)	)	-RRB-
cord-048471-7jszm1nd	99	20	.	.	.
cord-048471-7jszm1nd	100	1	Lysates	Lysates	NNP
cord-048471-7jszm1nd	100	2	of	of	IN
cord-048471-7jszm1nd	100	3	cells	cell	NNS
cord-048471-7jszm1nd	100	4	transfected	transfecte	VBN
cord-048471-7jszm1nd	100	5	with	with	IN
cord-048471-7jszm1nd	100	6	wild	wild	JJ
cord-048471-7jszm1nd	100	7	type	type	NN
cord-048471-7jszm1nd	100	8	JV	JV	NNP
cord-048471-7jszm1nd	100	9	and	and	CC
cord-048471-7jszm1nd	100	10	JV	JV	NNP
cord-048471-7jszm1nd	100	11	/	/	SYM
cord-048471-7jszm1nd	100	12	V5	V5	NNP
cord-048471-7jszm1nd	100	13	RNA	RNA	NNP
cord-048471-7jszm1nd	100	14	were	be	VBD
cord-048471-7jszm1nd	100	15	subjected	subject	VBN
cord-048471-7jszm1nd	100	16	to	to	IN
cord-048471-7jszm1nd	100	17	western	western	JJ
cord-048471-7jszm1nd	100	18	blot	blot	NN
cord-048471-7jszm1nd	100	19	using	use	VBG
cord-048471-7jszm1nd	100	20	the	the	DT
cord-048471-7jszm1nd	100	21	anti	anti	JJ
cord-048471-7jszm1nd	100	22	-	-	JJ
cord-048471-7jszm1nd	100	23	V5	v5	JJ
cord-048471-7jszm1nd	100	24	antibody	antibody	NN
cord-048471-7jszm1nd	100	25	(	(	-LRB-
cord-048471-7jszm1nd	100	26	Figure	Figure	NNP
cord-048471-7jszm1nd	100	27	7	7	CD
cord-048471-7jszm1nd	100	28	)	)	-RRB-
cord-048471-7jszm1nd	100	29	.	.	.
cord-048471-7jszm1nd	101	1	No	no	DT
cord-048471-7jszm1nd	101	2	product	product	NN
cord-048471-7jszm1nd	101	3	was	be	VBD
cord-048471-7jszm1nd	101	4	present	present	JJ
cord-048471-7jszm1nd	101	5	for	for	IN
cord-048471-7jszm1nd	101	6	cells	cell	NNS
cord-048471-7jszm1nd	101	7	transfected	transfecte	VBN
cord-048471-7jszm1nd	101	8	with	with	IN
cord-048471-7jszm1nd	101	9	wild	wild	JJ
cord-048471-7jszm1nd	101	10	type	type	NN
cord-048471-7jszm1nd	101	11	JV	JV	NNP
cord-048471-7jszm1nd	101	12	RNA	RNA	NNP
cord-048471-7jszm1nd	101	13	,	,	,
cord-048471-7jszm1nd	101	14	but	but	CC
cord-048471-7jszm1nd	101	15	a	a	DT
cord-048471-7jszm1nd	101	16	protein	protein	NN
cord-048471-7jszm1nd	101	17	of	of	IN
cord-048471-7jszm1nd	101	18	approximately	approximately	RB
cord-048471-7jszm1nd	101	19	42	42	CD
cord-048471-7jszm1nd	101	20	kDa	kDa	NNS
cord-048471-7jszm1nd	101	21	in	in	IN
cord-048471-7jszm1nd	101	22	size	size	NN
cord-048471-7jszm1nd	101	23	was	be	VBD
cord-048471-7jszm1nd	101	24	visible	visible	JJ
cord-048471-7jszm1nd	101	25	in	in	IN
cord-048471-7jszm1nd	101	26	cells	cell	NNS
cord-048471-7jszm1nd	101	27	that	that	WDT
cord-048471-7jszm1nd	101	28	had	have	VBD
cord-048471-7jszm1nd	101	29	been	be	VBN
cord-048471-7jszm1nd	101	30	transfected	transfecte	VBN
cord-048471-7jszm1nd	101	31	with	with	IN
cord-048471-7jszm1nd	101	32	JV	JV	NNP
cord-048471-7jszm1nd	101	33	/	/	SYM
cord-048471-7jszm1nd	101	34	V5	V5	NNP
cord-048471-7jszm1nd	101	35	RNA	RNA	NNP
cord-048471-7jszm1nd	101	36	,	,	,
cord-048471-7jszm1nd	101	37	confirming	confirm	VBG
cord-048471-7jszm1nd	101	38	N	n	CD
cord-048471-7jszm1nd	101	39	-	-	HYPH
cord-048471-7jszm1nd	101	40	term	term	NN
cord-048471-7jszm1nd	101	41	expression	expression	NN
cord-048471-7jszm1nd	101	42	and	and	CC
cord-048471-7jszm1nd	101	43	size	size	NN
cord-048471-7jszm1nd	101	44	as	as	IN
cord-048471-7jszm1nd	101	45	seen	see	VBN
cord-048471-7jszm1nd	101	46	in	in	IN
cord-048471-7jszm1nd	101	47	the	the	DT
cord-048471-7jszm1nd	101	48	in	in	FW
cord-048471-7jszm1nd	101	49	vitro	vitro	FW
cord-048471-7jszm1nd	101	50	system	system	NN
cord-048471-7jszm1nd	101	51	.	.	.
cord-048471-7jszm1nd	102	1	In	in	IN
cord-048471-7jszm1nd	102	2	addition	addition	NN
cord-048471-7jszm1nd	102	3	,	,	,
cord-048471-7jszm1nd	102	4	this	this	DT
cord-048471-7jszm1nd	102	5	important	important	JJ
cord-048471-7jszm1nd	102	6	observation	observation	NN
cord-048471-7jszm1nd	102	7	also	also	RB
cord-048471-7jszm1nd	102	8	confirms	confirm	VBZ
cord-048471-7jszm1nd	102	9	for	for	IN
cord-048471-7jszm1nd	102	10	the	the	DT
cord-048471-7jszm1nd	102	11	first	first	JJ
cord-048471-7jszm1nd	102	12	time	time	NN
cord-048471-7jszm1nd	102	13	that	that	WDT
cord-048471-7jszm1nd	102	14	the	the	DT
cord-048471-7jszm1nd	102	15	JV	JV	NNP
cord-048471-7jszm1nd	102	16	3C	3c	NN
cord-048471-7jszm1nd	102	17	protease	protease	NN
cord-048471-7jszm1nd	102	18	was	be	VBD
cord-048471-7jszm1nd	102	19	active	active	JJ
cord-048471-7jszm1nd	102	20	in	in	IN
cord-048471-7jszm1nd	102	21	cells	cell	NNS
cord-048471-7jszm1nd	102	22	transfected	transfecte	VBN
cord-048471-7jszm1nd	102	23	with	with	IN
cord-048471-7jszm1nd	102	24	capped	cap	VBN
cord-048471-7jszm1nd	102	25	RNA	RNA	NNP
cord-048471-7jszm1nd	102	26	as	as	IN
cord-048471-7jszm1nd	102	27	the	the	DT
cord-048471-7jszm1nd	102	28	size	size	NN
cord-048471-7jszm1nd	102	29	of	of	IN
cord-048471-7jszm1nd	102	30	the	the	DT
cord-048471-7jszm1nd	102	31	V5	V5	NNP
cord-048471-7jszm1nd	102	32	/	/	SYM
cord-048471-7jszm1nd	102	33	N	n	CD
cord-048471-7jszm1nd	102	34	-	-	HYPH
cord-048471-7jszm1nd	102	35	term	term	NN
cord-048471-7jszm1nd	102	36	indicated	indicate	VBD
cord-048471-7jszm1nd	102	37	successful	successful	JJ
cord-048471-7jszm1nd	102	38	cleavage	cleavage	NN
cord-048471-7jszm1nd	102	39	of	of	IN
cord-048471-7jszm1nd	102	40	the	the	DT
cord-048471-7jszm1nd	102	41	protein	protein	NN
cord-048471-7jszm1nd	102	42	from	from	IN
cord-048471-7jszm1nd	102	43	the	the	DT
cord-048471-7jszm1nd	102	44	ORF1	ORF1	NNP
cord-048471-7jszm1nd	102	45	polyprotein	polyprotein	NN
cord-048471-7jszm1nd	102	46	.	.	.
cord-048471-7jszm1nd	103	1	To	to	TO
cord-048471-7jszm1nd	103	2	address	address	VB
cord-048471-7jszm1nd	103	3	the	the	DT
cord-048471-7jszm1nd	103	4	issue	issue	NN
cord-048471-7jszm1nd	103	5	of	of	IN
cord-048471-7jszm1nd	103	6	potential	potential	JJ
cord-048471-7jszm1nd	103	7	rapid	rapid	JJ
cord-048471-7jszm1nd	103	8	degradation	degradation	NN
cord-048471-7jszm1nd	103	9	of	of	IN
cord-048471-7jszm1nd	103	10	the	the	DT
cord-048471-7jszm1nd	103	11	JV	JV	NNP
cord-048471-7jszm1nd	103	12	Nterm	Nterm	NNP
cord-048471-7jszm1nd	103	13	protein	protein	NN
cord-048471-7jszm1nd	103	14	CRFK	CRFK	NNP
cord-048471-7jszm1nd	103	15	cells	cell	NNS
cord-048471-7jszm1nd	103	16	were	be	VBD
cord-048471-7jszm1nd	103	17	transfected	transfecte	VBN
cord-048471-7jszm1nd	103	18	with	with	IN
cord-048471-7jszm1nd	103	19	JV	JV	NNP
cord-048471-7jszm1nd	103	20	V5	V5	NNP
cord-048471-7jszm1nd	103	21	RNA	RNA	NNP
cord-048471-7jszm1nd	103	22	and	and	CC
cord-048471-7jszm1nd	103	23	were	be	VBD
cord-048471-7jszm1nd	103	24	harvested	harvest	VBN
cord-048471-7jszm1nd	103	25	at	at	IN
cord-048471-7jszm1nd	103	26	designated	designate	VBN
cord-048471-7jszm1nd	103	27	time	time	NN
cord-048471-7jszm1nd	103	28	points	point	NNS
cord-048471-7jszm1nd	103	29	following	follow	VBG
cord-048471-7jszm1nd	103	30	the	the	DT
cord-048471-7jszm1nd	103	31	addition	addition	NN
cord-048471-7jszm1nd	103	32	of	of	IN
cord-048471-7jszm1nd	103	33	the	the	DT
cord-048471-7jszm1nd	103	34	protein	protein	NN
cord-048471-7jszm1nd	103	35	synthesis	synthesis	NN
cord-048471-7jszm1nd	103	36	inhibitor	inhibitor	NN
cord-048471-7jszm1nd	103	37	cycloheximide	cycloheximide	NNP
cord-048471-7jszm1nd	103	38	.	.	.
cord-048471-7jszm1nd	104	1	Cell	cell	NN
cord-048471-7jszm1nd	104	2	lysates	lysate	NNS
cord-048471-7jszm1nd	104	3	were	be	VBD
cord-048471-7jszm1nd	104	4	analysed	analyse	VBN
cord-048471-7jszm1nd	104	5	by	by	IN
cord-048471-7jszm1nd	104	6	Western	western	JJ
cord-048471-7jszm1nd	104	7	blot	blot	NN
cord-048471-7jszm1nd	104	8	using	use	VBG
cord-048471-7jszm1nd	104	9	the	the	DT
cord-048471-7jszm1nd	104	10	anti	anti	JJ
cord-048471-7jszm1nd	104	11	-	-	JJ
cord-048471-7jszm1nd	104	12	V5	v5	JJ
cord-048471-7jszm1nd	104	13	antibody	antibody	NN
cord-048471-7jszm1nd	104	14	(	(	-LRB-
cord-048471-7jszm1nd	104	15	Figure	Figure	NNP
cord-048471-7jszm1nd	104	16	8	8	CD
cord-048471-7jszm1nd	104	17	)	)	-RRB-
cord-048471-7jszm1nd	104	18	.	.	.
cord-048471-7jszm1nd	105	1	The	the	DT
cord-048471-7jszm1nd	105	2	consistent	consistent	JJ
cord-048471-7jszm1nd	105	3	appearance	appearance	NN
cord-048471-7jszm1nd	105	4	of	of	IN
cord-048471-7jszm1nd	105	5	the	the	DT
cord-048471-7jszm1nd	105	6	N	n	CD
cord-048471-7jszm1nd	105	7	-	-	HYPH
cord-048471-7jszm1nd	105	8	term	term	NN
cord-048471-7jszm1nd	105	9	/	/	SYM
cord-048471-7jszm1nd	105	10	V5	v5	NN
cord-048471-7jszm1nd	105	11	protein	protein	NN
cord-048471-7jszm1nd	105	12	suggested	suggest	VBD
cord-048471-7jszm1nd	105	13	that	that	IN
cord-048471-7jszm1nd	105	14	it	-PRON-	PRP
cord-048471-7jszm1nd	105	15	is	be	VBZ
cord-048471-7jszm1nd	105	16	stable	stable	JJ
cord-048471-7jszm1nd	105	17	and	and	CC
cord-048471-7jszm1nd	105	18	insensitive	insensitive	JJ
cord-048471-7jszm1nd	105	19	to	to	IN
cord-048471-7jszm1nd	105	20	degradation	degradation	NN
cord-048471-7jszm1nd	105	21	by	by	IN
cord-048471-7jszm1nd	105	22	viral	viral	JJ
cord-048471-7jszm1nd	105	23	and	and	CC
cord-048471-7jszm1nd	105	24	host	host	NN
cord-048471-7jszm1nd	105	25	cell	cell	NN
cord-048471-7jszm1nd	105	26	proteases	protease	NNS
cord-048471-7jszm1nd	105	27	.	.	.
cord-048471-7jszm1nd	106	1	The	the	DT
cord-048471-7jszm1nd	106	2	predicted	predict	VBN
cord-048471-7jszm1nd	106	3	molecular	molecular	JJ
cord-048471-7jszm1nd	106	4	weight	weight	NN
cord-048471-7jszm1nd	106	5	of	of	IN
cord-048471-7jszm1nd	106	6	the	the	DT
cord-048471-7jszm1nd	106	7	JV	JV	NNP
cord-048471-7jszm1nd	106	8	N	N	NNP
cord-048471-7jszm1nd	106	9	-	-	HYPH
cord-048471-7jszm1nd	106	10	term	term	NN
cord-048471-7jszm1nd	106	11	is	be	VBZ
cord-048471-7jszm1nd	106	12	35.3	35.3	CD
cord-048471-7jszm1nd	106	13	kDa	kDa	NNP
cord-048471-7jszm1nd	106	14	,	,	,
cord-048471-7jszm1nd	106	15	based	base	VBN
cord-048471-7jszm1nd	106	16	on	on	IN
cord-048471-7jszm1nd	106	17	the	the	DT
cord-048471-7jszm1nd	106	18	site	site	NN
cord-048471-7jszm1nd	106	19	of	of	IN
cord-048471-7jszm1nd	106	20	initiation	initiation	NN
cord-048471-7jszm1nd	106	21	of	of	IN
cord-048471-7jszm1nd	106	22	translation	translation	NN
cord-048471-7jszm1nd	106	23	and	and	CC
cord-048471-7jszm1nd	106	24	location	location	NN
cord-048471-7jszm1nd	106	25	of	of	IN
cord-048471-7jszm1nd	106	26	conserved	conserved	JJ
cord-048471-7jszm1nd	106	27	cleavage	cleavage	NN
cord-048471-7jszm1nd	106	28	sites	site	NNS
cord-048471-7jszm1nd	106	29	.	.	.
cord-048471-7jszm1nd	107	1	The	the	DT
cord-048471-7jszm1nd	107	2	appearance	appearance	NN
cord-048471-7jszm1nd	107	3	,	,	,
cord-048471-7jszm1nd	107	4	therefore	therefore	RB
cord-048471-7jszm1nd	107	5	,	,	,
cord-048471-7jszm1nd	107	6	of	of	IN
cord-048471-7jszm1nd	107	7	a	a	DT
cord-048471-7jszm1nd	107	8	previously	previously	RB
cord-048471-7jszm1nd	107	9	unseen	unseen	JJ
cord-048471-7jszm1nd	107	10	42	42	CD
cord-048471-7jszm1nd	107	11	kDa	kDa	NNS
cord-048471-7jszm1nd	107	12	protein	protein	NN
cord-048471-7jszm1nd	107	13	in	in	IN
cord-048471-7jszm1nd	107	14	the	the	DT
cord-048471-7jszm1nd	107	15	in	in	FW
cord-048471-7jszm1nd	107	16	vitro	vitro	FW
cord-048471-7jszm1nd	107	17	transcription	transcription	NN
cord-048471-7jszm1nd	107	18	and	and	CC
cord-048471-7jszm1nd	107	19	translation	translation	NN
cord-048471-7jszm1nd	107	20	profile	profile	NN
cord-048471-7jszm1nd	107	21	was	be	VBD
cord-048471-7jszm1nd	107	22	unexpected	unexpected	JJ
cord-048471-7jszm1nd	107	23	but	but	CC
cord-048471-7jszm1nd	107	24	this	this	DT
cord-048471-7jszm1nd	107	25	protein	protein	NN
cord-048471-7jszm1nd	107	26	does	do	VBZ
cord-048471-7jszm1nd	107	27	represent	represent	VB
cord-048471-7jszm1nd	107	28	a	a	DT
cord-048471-7jszm1nd	107	29	translation	translation	NN
cord-048471-7jszm1nd	107	30	product	product	NN
cord-048471-7jszm1nd	107	31	for	for	IN
cord-048471-7jszm1nd	107	32	the	the	DT
cord-048471-7jszm1nd	107	33	JV	JV	NNP
cord-048471-7jszm1nd	107	34	59GS	59gs	CD
cord-048471-7jszm1nd	107	35	.	.	.
cord-048471-7jszm1nd	108	1	To	to	IN
cord-048471-7jszm1nd	108	2	date	date	NN
cord-048471-7jszm1nd	108	3	,	,	,
cord-048471-7jszm1nd	108	4	it	-PRON-	PRP
cord-048471-7jszm1nd	108	5	has	have	VBZ
cord-048471-7jszm1nd	108	6	not	not	RB
cord-048471-7jszm1nd	108	7	been	be	VBN
cord-048471-7jszm1nd	108	8	possible	possible	JJ
cord-048471-7jszm1nd	108	9	to	to	TO
cord-048471-7jszm1nd	108	10	explain	explain	VB
cord-048471-7jszm1nd	108	11	the	the	DT
cord-048471-7jszm1nd	108	12	difference	difference	NN
cord-048471-7jszm1nd	108	13	in	in	IN
cord-048471-7jszm1nd	108	14	the	the	DT
cord-048471-7jszm1nd	108	15	predicted	predict	VBN
cord-048471-7jszm1nd	108	16	and	and	CC
cord-048471-7jszm1nd	108	17	observed	observe	VBD
cord-048471-7jszm1nd	108	18	sizes	size	NNS
cord-048471-7jszm1nd	108	19	for	for	IN
cord-048471-7jszm1nd	108	20	the	the	DT
cord-048471-7jszm1nd	108	21	JV	JV	NNP
cord-048471-7jszm1nd	108	22	N	N	NNP
cord-048471-7jszm1nd	108	23	-	-	HYPH
cord-048471-7jszm1nd	108	24	term	term	NN
cord-048471-7jszm1nd	108	25	,	,	,
cord-048471-7jszm1nd	108	26	and	and	CC
cord-048471-7jszm1nd	108	27	the	the	DT
cord-048471-7jszm1nd	108	28	addition	addition	NN
cord-048471-7jszm1nd	108	29	of	of	IN
cord-048471-7jszm1nd	108	30	the	the	DT
cord-048471-7jszm1nd	108	31	14	14	CD
cord-048471-7jszm1nd	108	32	amino	amino	NN
cord-048471-7jszm1nd	108	33	acid	acid	NN
cord-048471-7jszm1nd	108	34	V5	v5	NN
cord-048471-7jszm1nd	108	35	epitope	epitope	NN
cord-048471-7jszm1nd	108	36	within	within	IN
cord-048471-7jszm1nd	108	37	JV	JV	NNP
cord-048471-7jszm1nd	108	38	N	N	NNP
cord-048471-7jszm1nd	108	39	-	-	HYPH
cord-048471-7jszm1nd	108	40	term	term	NN
cord-048471-7jszm1nd	108	41	does	do	VBZ
cord-048471-7jszm1nd	108	42	not	not	RB
cord-048471-7jszm1nd	108	43	account	account	VB
cord-048471-7jszm1nd	108	44	for	for	IN
cord-048471-7jszm1nd	108	45	this	this	DT
cord-048471-7jszm1nd	108	46	apparent	apparent	JJ
cord-048471-7jszm1nd	108	47	large	large	JJ
cord-048471-7jszm1nd	108	48	shift	shift	NN
cord-048471-7jszm1nd	108	49	in	in	IN
cord-048471-7jszm1nd	108	50	molecular	molecular	JJ
cord-048471-7jszm1nd	108	51	weight	weight	NN
cord-048471-7jszm1nd	108	52	.	.	.
cord-048471-7jszm1nd	109	1	However	however	RB
cord-048471-7jszm1nd	109	2	,	,	,
cord-048471-7jszm1nd	109	3	a	a	DT
cord-048471-7jszm1nd	109	4	recent	recent	JJ
cord-048471-7jszm1nd	109	5	study	study	NN
cord-048471-7jszm1nd	109	6	described	describe	VBD
cord-048471-7jszm1nd	109	7	a	a	DT
cord-048471-7jszm1nd	109	8	similar	similar	JJ
cord-048471-7jszm1nd	109	9	anomaly	anomaly	NN
cord-048471-7jszm1nd	109	10	when	when	WRB
cord-048471-7jszm1nd	109	11	investigating	investigate	VBG
cord-048471-7jszm1nd	109	12	proteolytic	proteolytic	JJ
cord-048471-7jszm1nd	109	13	processing	processing	NN
cord-048471-7jszm1nd	109	14	in	in	IN
cord-048471-7jszm1nd	109	15	the	the	DT
cord-048471-7jszm1nd	109	16	murine	murine	JJ
cord-048471-7jszm1nd	109	17	norovirus	norovirus	NN
cord-048471-7jszm1nd	109	18	MNV-1	MNV-1	NNP
cord-048471-7jszm1nd	109	19	[	[	-LRB-
cord-048471-7jszm1nd	109	20	12	12	CD
cord-048471-7jszm1nd	109	21	]	]	-RRB-
cord-048471-7jszm1nd	109	22	.	.	.
cord-048471-7jszm1nd	110	1	The	the	DT
cord-048471-7jszm1nd	110	2	predicted	predict	VBN
cord-048471-7jszm1nd	110	3	molecular	molecular	JJ
cord-048471-7jszm1nd	110	4	weight	weight	NN
cord-048471-7jszm1nd	110	5	for	for	IN
cord-048471-7jszm1nd	110	6	the	the	DT
cord-048471-7jszm1nd	110	7	MNV-1	MNV-1	NNP
cord-048471-7jszm1nd	110	8	N	n	CD
cord-048471-7jszm1nd	110	9	-	-	HYPH
cord-048471-7jszm1nd	110	10	term	term	NN
cord-048471-7jszm1nd	110	11	protein	protein	NN
cord-048471-7jszm1nd	110	12	was	be	VBD
cord-048471-7jszm1nd	110	13	38.3	38.3	CD
cord-048471-7jszm1nd	110	14	kDa	kDa	NNS
cord-048471-7jszm1nd	110	15	.	.	.
cord-048471-7jszm1nd	111	1	The	the	DT
cord-048471-7jszm1nd	111	2	authors	author	NNS
cord-048471-7jszm1nd	111	3	successfully	successfully	RB
cord-048471-7jszm1nd	111	4	generated	generate	VBD
cord-048471-7jszm1nd	111	5	antisera	antisera	NN
cord-048471-7jszm1nd	111	6	against	against	IN
cord-048471-7jszm1nd	111	7	the	the	DT
cord-048471-7jszm1nd	111	8	MNV-1	MNV-1	NNP
cord-048471-7jszm1nd	111	9	N	n	CD
cord-048471-7jszm1nd	111	10	-	-	HYPH
cord-048471-7jszm1nd	111	11	term	term	NN
cord-048471-7jszm1nd	111	12	and	and	CC
cord-048471-7jszm1nd	111	13	used	use	VBD
cord-048471-7jszm1nd	111	14	it	-PRON-	PRP
cord-048471-7jszm1nd	111	15	to	to	TO
cord-048471-7jszm1nd	111	16	immunoprecipitate	immunoprecipitate	VB
cord-048471-7jszm1nd	111	17	the	the	DT
cord-048471-7jszm1nd	111	18	protein	protein	NN
cord-048471-7jszm1nd	111	19	from	from	IN
cord-048471-7jszm1nd	111	20	in	in	IN
cord-048471-7jszm1nd	111	21	vitro	vitro	FW
cord-048471-7jszm1nd	111	22	transcription	transcription	NN
cord-048471-7jszm1nd	111	23	and	and	CC
cord-048471-7jszm1nd	111	24	translation	translation	NN
cord-048471-7jszm1nd	111	25	reactions	reaction	NNS
cord-048471-7jszm1nd	111	26	and	and	CC
cord-048471-7jszm1nd	111	27	observed	observe	VBD
cord-048471-7jszm1nd	111	28	that	that	IN
cord-048471-7jszm1nd	111	29	the	the	DT
cord-048471-7jszm1nd	111	30	N	n	CD
cord-048471-7jszm1nd	111	31	-	-	HYPH
cord-048471-7jszm1nd	111	32	term	term	NN
cord-048471-7jszm1nd	111	33	existed	exist	VBD
cord-048471-7jszm1nd	111	34	as	as	IN
cord-048471-7jszm1nd	111	35	a	a	DT
cord-048471-7jszm1nd	111	36	45	45	CD
cord-048471-7jszm1nd	111	37	kDa	kDa	NNS
cord-048471-7jszm1nd	111	38	doublet	doublet	NN
cord-048471-7jszm1nd	111	39	,	,	,
cord-048471-7jszm1nd	111	40	in	in	IN
cord-048471-7jszm1nd	111	41	addition	addition	NN
cord-048471-7jszm1nd	111	42	to	to	IN
cord-048471-7jszm1nd	111	43	the	the	DT
cord-048471-7jszm1nd	111	44	predicted	predict	VBN
cord-048471-7jszm1nd	111	45	size	size	NN
cord-048471-7jszm1nd	111	46	of	of	IN
cord-048471-7jszm1nd	111	47	38	38	CD
cord-048471-7jszm1nd	111	48	kDa	kDa	NNS
cord-048471-7jszm1nd	111	49	.	.	.
cord-048471-7jszm1nd	112	1	However	however	RB
cord-048471-7jszm1nd	112	2	,	,	,
cord-048471-7jszm1nd	112	3	when	when	WRB
cord-048471-7jszm1nd	112	4	MNV-1	MNV-1	NNP
cord-048471-7jszm1nd	112	5	N	n	CD
cord-048471-7jszm1nd	112	6	-	-	HYPH
cord-048471-7jszm1nd	112	7	term	term	NN
cord-048471-7jszm1nd	112	8	antisera	antisera	NN
cord-048471-7jszm1nd	112	9	was	be	VBD
cord-048471-7jszm1nd	112	10	used	use	VBN
cord-048471-7jszm1nd	112	11	to	to	TO
cord-048471-7jszm1nd	112	12	probe	probe	VB
cord-048471-7jszm1nd	112	13	MNV-1-infected	mnv-1-infected	JJ
cord-048471-7jszm1nd	112	14	cell	cell	NN
cord-048471-7jszm1nd	112	15	lysates	lysate	NNS
cord-048471-7jszm1nd	112	16	only	only	RB
cord-048471-7jszm1nd	112	17	the	the	DT
cord-048471-7jszm1nd	112	18	43	43	CD
cord-048471-7jszm1nd	112	19	-	-	SYM
cord-048471-7jszm1nd	112	20	45	45	CD
cord-048471-7jszm1nd	112	21	kDa	kDa	NNS
cord-048471-7jszm1nd	112	22	doublet	doublet	NN
cord-048471-7jszm1nd	112	23	and	and	CC
cord-048471-7jszm1nd	112	24	a	a	DT
cord-048471-7jszm1nd	112	25	large	large	JJ
cord-048471-7jszm1nd	112	26	115	115	CD
cord-048471-7jszm1nd	112	27	kDa	kDa	NNS
cord-048471-7jszm1nd	112	28	precursor	precursor	NN
cord-048471-7jszm1nd	112	29	could	could	MD
cord-048471-7jszm1nd	112	30	be	be	VB
cord-048471-7jszm1nd	112	31	detected	detect	VBN
cord-048471-7jszm1nd	112	32	,	,	,
cord-048471-7jszm1nd	112	33	suggesting	suggest	VBG
cord-048471-7jszm1nd	112	34	that	that	IN
cord-048471-7jszm1nd	112	35	the	the	DT
cord-048471-7jszm1nd	112	36	predicted	predict	VBN
cord-048471-7jszm1nd	112	37	38	38	CD
cord-048471-7jszm1nd	112	38	kDa	kDa	NNP
cord-048471-7jszm1nd	112	39	form	form	NN
cord-048471-7jszm1nd	112	40	of	of	IN
cord-048471-7jszm1nd	112	41	the	the	DT
cord-048471-7jszm1nd	112	42	N	n	CD
cord-048471-7jszm1nd	112	43	-	-	HYPH
cord-048471-7jszm1nd	112	44	term	term	NN
cord-048471-7jszm1nd	112	45	is	be	VBZ
cord-048471-7jszm1nd	112	46	not	not	RB
cord-048471-7jszm1nd	112	47	generated	generate	VBN
cord-048471-7jszm1nd	112	48	in	in	IN
cord-048471-7jszm1nd	112	49	cell	cell	NN
cord-048471-7jszm1nd	112	50	culture	culture	NN
cord-048471-7jszm1nd	112	51	.	.	.
cord-048471-7jszm1nd	113	1	Again	again	RB
cord-048471-7jszm1nd	113	2	,	,	,
cord-048471-7jszm1nd	113	3	it	-PRON-	PRP
cord-048471-7jszm1nd	113	4	was	be	VBD
cord-048471-7jszm1nd	113	5	not	not	RB
cord-048471-7jszm1nd	113	6	possible	possible	JJ
cord-048471-7jszm1nd	113	7	to	to	TO
cord-048471-7jszm1nd	113	8	conclusively	conclusively	RB
cord-048471-7jszm1nd	113	9	determine	determine	VB
cord-048471-7jszm1nd	113	10	the	the	DT
cord-048471-7jszm1nd	113	11	cause	cause	NN
cord-048471-7jszm1nd	113	12	of	of	IN
cord-048471-7jszm1nd	113	13	this	this	DT
cord-048471-7jszm1nd	113	14	discrepancy	discrepancy	NN
cord-048471-7jszm1nd	113	15	,	,	,
cord-048471-7jszm1nd	113	16	but	but	CC
cord-048471-7jszm1nd	113	17	it	-PRON-	PRP
cord-048471-7jszm1nd	113	18	was	be	VBD
cord-048471-7jszm1nd	113	19	speculated	speculate	VBN
cord-048471-7jszm1nd	113	20	that	that	IN
cord-048471-7jszm1nd	113	21	the	the	DT
cord-048471-7jszm1nd	113	22	N	n	CD
cord-048471-7jszm1nd	113	23	-	-	HYPH
cord-048471-7jszm1nd	113	24	term	term	NN
cord-048471-7jszm1nd	113	25	protein	protein	NN
cord-048471-7jszm1nd	113	26	may	may	MD
cord-048471-7jszm1nd	113	27	migrate	migrate	VB
cord-048471-7jszm1nd	113	28	abnormally	abnormally	RB
cord-048471-7jszm1nd	113	29	in	in	IN
cord-048471-7jszm1nd	113	30	SDS	SDS	NNP
cord-048471-7jszm1nd	113	31	-	-	HYPH
cord-048471-7jszm1nd	113	32	PAGE	PAGE	NNP
cord-048471-7jszm1nd	113	33	,	,	,
cord-048471-7jszm1nd	113	34	or	or	CC
cord-048471-7jszm1nd	113	35	may	may	MD
cord-048471-7jszm1nd	113	36	be	be	VB
cord-048471-7jszm1nd	113	37	proteolytically	proteolytically	RB
cord-048471-7jszm1nd	113	38	processed	process	VBN
cord-048471-7jszm1nd	113	39	at	at	IN
cord-048471-7jszm1nd	113	40	a	a	DT
cord-048471-7jszm1nd	113	41	previously	previously	RB
cord-048471-7jszm1nd	113	42	unknown	unknown	JJ
cord-048471-7jszm1nd	113	43	cleavage	cleavage	NN
cord-048471-7jszm1nd	113	44	site	site	NN
cord-048471-7jszm1nd	113	45	downstream	downstream	NN
cord-048471-7jszm1nd	113	46	of	of	IN
cord-048471-7jszm1nd	113	47	the	the	DT
cord-048471-7jszm1nd	113	48	protein	protein	NN
cord-048471-7jszm1nd	113	49	's	's	POS
cord-048471-7jszm1nd	113	50	predicted	predict	VBN
cord-048471-7jszm1nd	113	51	C	c	NN
cord-048471-7jszm1nd	113	52	-	-	HYPH
cord-048471-7jszm1nd	113	53	terminus	terminus	NN
cord-048471-7jszm1nd	113	54	.	.	.
cord-048471-7jszm1nd	114	1	It	-PRON-	PRP
cord-048471-7jszm1nd	114	2	is	be	VBZ
cord-048471-7jszm1nd	114	3	also	also	RB
cord-048471-7jszm1nd	114	4	possible	possible	JJ
cord-048471-7jszm1nd	114	5	that	that	IN
cord-048471-7jszm1nd	114	6	the	the	DT
cord-048471-7jszm1nd	114	7	N	n	CD
cord-048471-7jszm1nd	114	8	-	-	HYPH
cord-048471-7jszm1nd	114	9	term	term	NN
cord-048471-7jszm1nd	114	10	protein	protein	NN
cord-048471-7jszm1nd	114	11	might	may	MD
cord-048471-7jszm1nd	114	12	be	be	VB
cord-048471-7jszm1nd	114	13	modified	modify	VBN
cord-048471-7jszm1nd	114	14	in	in	IN
cord-048471-7jszm1nd	114	15	some	some	DT
cord-048471-7jszm1nd	114	16	way	way	NN
cord-048471-7jszm1nd	114	17	leading	lead	VBG
cord-048471-7jszm1nd	114	18	to	to	IN
cord-048471-7jszm1nd	114	19	a	a	DT
cord-048471-7jszm1nd	114	20	shift	shift	NN
cord-048471-7jszm1nd	114	21	in	in	IN
cord-048471-7jszm1nd	114	22	observed	observed	JJ
cord-048471-7jszm1nd	114	23	molecular	molecular	JJ
cord-048471-7jszm1nd	114	24	weight	weight	NN
cord-048471-7jszm1nd	114	25	.	.	.
cord-048471-7jszm1nd	115	1	At	at	IN
cord-048471-7jszm1nd	115	2	this	this	DT
cord-048471-7jszm1nd	115	3	time	time	NN
cord-048471-7jszm1nd	115	4	the	the	DT
cord-048471-7jszm1nd	115	5	same	same	JJ
cord-048471-7jszm1nd	115	6	conclusions	conclusion	NNS
cord-048471-7jszm1nd	115	7	would	would	MD
cord-048471-7jszm1nd	115	8	seem	seem	VB
cord-048471-7jszm1nd	115	9	appropriate	appropriate	JJ
cord-048471-7jszm1nd	115	10	for	for	IN
cord-048471-7jszm1nd	115	11	the	the	DT
cord-048471-7jszm1nd	115	12	JV	JV	NNP
cord-048471-7jszm1nd	115	13	N	N	NNP
cord-048471-7jszm1nd	115	14	-	-	HYPH
cord-048471-7jszm1nd	115	15	term	term	NN
cord-048471-7jszm1nd	115	16	.	.	.
cord-048471-7jszm1nd	116	1	In	in	IN
cord-048471-7jszm1nd	116	2	addition	addition	NN
cord-048471-7jszm1nd	116	3	,	,	,
cord-048471-7jszm1nd	116	4	it	-PRON-	PRP
cord-048471-7jszm1nd	116	5	is	be	VBZ
cord-048471-7jszm1nd	116	6	not	not	RB
cord-048471-7jszm1nd	116	7	known	know	VBN
cord-048471-7jszm1nd	116	8	why	why	WRB
cord-048471-7jszm1nd	116	9	the	the	DT
cord-048471-7jszm1nd	116	10	JV	JV	NNP
cord-048471-7jszm1nd	116	11	N	N	NNP
cord-048471-7jszm1nd	116	12	-	-	HYPH
cord-048471-7jszm1nd	116	13	term	term	NN
cord-048471-7jszm1nd	116	14	was	be	VBD
cord-048471-7jszm1nd	116	15	previously	previously	RB
cord-048471-7jszm1nd	116	16	not	not	RB
cord-048471-7jszm1nd	116	17	detected	detect	VBN
cord-048471-7jszm1nd	116	18	in	in	RP
cord-048471-7jszm1nd	116	19	in	in	IN
cord-048471-7jszm1nd	116	20	vitro	vitro	FW
cord-048471-7jszm1nd	116	21	transcription	transcription	NN
cord-048471-7jszm1nd	116	22	and	and	CC
cord-048471-7jszm1nd	116	23	translation	translation	NN
cord-048471-7jszm1nd	116	24	studies	study	NNS
cord-048471-7jszm1nd	116	25	prior	prior	RB
cord-048471-7jszm1nd	116	26	to	to	IN
cord-048471-7jszm1nd	116	27	the	the	DT
cord-048471-7jszm1nd	116	28	insertion	insertion	NN
cord-048471-7jszm1nd	116	29	of	of	IN
cord-048471-7jszm1nd	116	30	the	the	DT
cord-048471-7jszm1nd	116	31	V5	V5	NNP
cord-048471-7jszm1nd	116	32	epitope	epitope	NN
cord-048471-7jszm1nd	116	33	.	.	.
cord-048471-7jszm1nd	117	1	It	-PRON-	PRP
cord-048471-7jszm1nd	117	2	can	can	MD
cord-048471-7jszm1nd	117	3	not	not	RB
cord-048471-7jszm1nd	117	4	be	be	VB
cord-048471-7jszm1nd	117	5	ruled	rule	VBN
cord-048471-7jszm1nd	117	6	out	out	RP
cord-048471-7jszm1nd	117	7	,	,	,
cord-048471-7jszm1nd	117	8	however	however	RB
cord-048471-7jszm1nd	117	9	,	,	,
cord-048471-7jszm1nd	117	10	that	that	IN
cord-048471-7jszm1nd	117	11	the	the	DT
cord-048471-7jszm1nd	117	12	wild	wild	JJ
cord-048471-7jszm1nd	117	13	type	type	NN
cord-048471-7jszm1nd	117	14	JV	JV	NNP
cord-048471-7jszm1nd	117	15	N	N	NNP
cord-048471-7jszm1nd	117	16	-	-	HYPH
cord-048471-7jszm1nd	117	17	term	term	NN
cord-048471-7jszm1nd	117	18	aberrantly	aberrantly	RB
cord-048471-7jszm1nd	117	19	co	co	VBD
cord-048471-7jszm1nd	117	20	-	-	VBZ
cord-048471-7jszm1nd	117	21	migrates	migrate	VBZ
cord-048471-7jszm1nd	117	22	with	with	IN
cord-048471-7jszm1nd	117	23	the	the	DT
cord-048471-7jszm1nd	117	24	39	39	CD
cord-048471-7jszm1nd	117	25	kDa	kDa	NNP
cord-048471-7jszm1nd	117	26	JV	JV	NNP
cord-048471-7jszm1nd	117	27	2C	2c	NN
cord-048471-7jszm1nd	117	28	protein	protein	NN
cord-048471-7jszm1nd	117	29	in	in	IN
cord-048471-7jszm1nd	117	30	SDS	SDS	NNP
cord-048471-7jszm1nd	117	31	-	-	HYPH
cord-048471-7jszm1nd	117	32	PAGE	PAGE	NNP
cord-048471-7jszm1nd	117	33	.	.	.
cord-048471-7jszm1nd	118	1	Indeed	indeed	RB
cord-048471-7jszm1nd	118	2	,	,	,
cord-048471-7jszm1nd	118	3	the	the	DT
cord-048471-7jszm1nd	118	4	appearance	appearance	NN
cord-048471-7jszm1nd	118	5	of	of	IN
cord-048471-7jszm1nd	118	6	the	the	DT
cord-048471-7jszm1nd	118	7	V5/	V5/	NNP
cord-048471-7jszm1nd	118	8	N	N	NNP
cord-048471-7jszm1nd	118	9	-	-	HYPH
cord-048471-7jszm1nd	118	10	term	term	NN
cord-048471-7jszm1nd	118	11	product	product	NN
cord-048471-7jszm1nd	118	12	from	from	IN
cord-048471-7jszm1nd	118	13	transfected	transfecte	VBN
cord-048471-7jszm1nd	118	14	cell	cell	NN
cord-048471-7jszm1nd	118	15	lysates	lysate	NNS
cord-048471-7jszm1nd	118	16	would	would	MD
cord-048471-7jszm1nd	118	17	appear	appear	VB
cord-048471-7jszm1nd	118	18	to	to	TO
cord-048471-7jszm1nd	118	19	be	be	VB
cord-048471-7jszm1nd	118	20	one	one	CD
cord-048471-7jszm1nd	118	21	of	of	IN
cord-048471-7jszm1nd	118	22	a	a	DT
cord-048471-7jszm1nd	118	23	doublet	doublet	NN
cord-048471-7jszm1nd	118	24	(	(	-LRB-
cord-048471-7jszm1nd	118	25	Figure	figure	NN
cord-048471-7jszm1nd	118	26	8)	8)	NN
cord-048471-7jszm1nd	118	27	,	,	,
cord-048471-7jszm1nd	118	28	also	also	RB
cord-048471-7jszm1nd	118	29	analogous	analogous	JJ
cord-048471-7jszm1nd	118	30	to	to	IN
cord-048471-7jszm1nd	118	31	the	the	DT
cord-048471-7jszm1nd	118	32	observed	observed	JJ
cord-048471-7jszm1nd	118	33	appearance	appearance	NN
cord-048471-7jszm1nd	118	34	of	of	IN
cord-048471-7jszm1nd	118	35	the	the	DT
cord-048471-7jszm1nd	118	36	MNV	MNV	NNP
cord-048471-7jszm1nd	118	37	N	n	CD
cord-048471-7jszm1nd	118	38	-	-	HYPH
cord-048471-7jszm1nd	118	39	term	term	NN
cord-048471-7jszm1nd	118	40	protein	protein	NN
cord-048471-7jszm1nd	118	41	in	in	IN
cord-048471-7jszm1nd	118	42	infected	infected	JJ
cord-048471-7jszm1nd	118	43	cells	cell	NNS
cord-048471-7jszm1nd	118	44	,	,	,
cord-048471-7jszm1nd	118	45	suggesting	suggest	VBG
cord-048471-7jszm1nd	118	46	the	the	DT
cord-048471-7jszm1nd	118	47	likelihood	likelihood	NN
cord-048471-7jszm1nd	118	48	of	of	IN
cord-048471-7jszm1nd	118	49	a	a	DT
cord-048471-7jszm1nd	118	50	further	further	JJ
cord-048471-7jszm1nd	118	51	cleavage	cleavage	NN
cord-048471-7jszm1nd	118	52	site	site	NN
cord-048471-7jszm1nd	118	53	within	within	IN
cord-048471-7jszm1nd	118	54	the	the	DT
cord-048471-7jszm1nd	118	55	JV	JV	NNP
cord-048471-7jszm1nd	118	56	N	N	NNP
cord-048471-7jszm1nd	118	57	-	-	HYPH
cord-048471-7jszm1nd	118	58	term	term	NN
cord-048471-7jszm1nd	118	59	protein	protein	NN
cord-048471-7jszm1nd	118	60	which	which	WDT
cord-048471-7jszm1nd	118	61	has	have	VBZ
cord-048471-7jszm1nd	118	62	yet	yet	RB
cord-048471-7jszm1nd	118	63	to	to	TO
cord-048471-7jszm1nd	118	64	be	be	VB
cord-048471-7jszm1nd	118	65	elucidated	elucidate	VBN
cord-048471-7jszm1nd	118	66	.	.	.
cord-048471-7jszm1nd	119	1	Nevertheless	nevertheless	RB
cord-048471-7jszm1nd	119	2	,	,	,
cord-048471-7jszm1nd	119	3	these	these	DT
cord-048471-7jszm1nd	119	4	studies	study	NNS
cord-048471-7jszm1nd	119	5	clearly	clearly	RB
cord-048471-7jszm1nd	119	6	demonstrate	demonstrate	VBP
cord-048471-7jszm1nd	119	7	that	that	IN
cord-048471-7jszm1nd	119	8	a	a	DT
cord-048471-7jszm1nd	119	9	protein	protein	NN
cord-048471-7jszm1nd	119	10	representative	representative	NN
cord-048471-7jszm1nd	119	11	of	of	IN
cord-048471-7jszm1nd	119	12	the	the	DT
cord-048471-7jszm1nd	119	13	59GS	59gs	CD
cord-048471-7jszm1nd	119	14	of	of	IN
cord-048471-7jszm1nd	119	15	JV	JV	NNP
cord-048471-7jszm1nd	119	16	is	be	VBZ
cord-048471-7jszm1nd	119	17	translated	translate	VBN
cord-048471-7jszm1nd	119	18	both	both	CC
cord-048471-7jszm1nd	119	19	in	in	IN
cord-048471-7jszm1nd	119	20	vitro	vitro	FW
cord-048471-7jszm1nd	119	21	and	and	CC
cord-048471-7jszm1nd	119	22	in	in	IN
cord-048471-7jszm1nd	119	23	vivo	vivo	NN
cord-048471-7jszm1nd	119	24	and	and	CC
cord-048471-7jszm1nd	119	25	is	be	VBZ
cord-048471-7jszm1nd	119	26	proteolytically	proteolytically	RB
cord-048471-7jszm1nd	119	27	processed	process	VBN
cord-048471-7jszm1nd	119	28	from	from	IN
cord-048471-7jszm1nd	119	29	the	the	DT
cord-048471-7jszm1nd	119	30	ORF1	ORF1	NNP
cord-048471-7jszm1nd	119	31	polyprotein	polyprotein	NN
cord-048471-7jszm1nd	119	32	following	follow	VBG
cord-048471-7jszm1nd	119	33	translation	translation	NN
cord-048471-7jszm1nd	119	34	initiation	initiation	NN
cord-048471-7jszm1nd	119	35	at	at	IN
cord-048471-7jszm1nd	119	36	nucleotide	nucleotide	JJ
cord-048471-7jszm1nd	119	37	22	22	CD
cord-048471-7jszm1nd	119	38	.	.	.
cord-048471-7jszm1nd	120	1	Human	human	JJ
cord-048471-7jszm1nd	120	2	norovirus	norovirus	NN
cord-048471-7jszm1nd	120	3	infection	infection	NN
cord-048471-7jszm1nd	120	4	has	have	VBZ
cord-048471-7jszm1nd	120	5	been	be	VBN
cord-048471-7jszm1nd	120	6	shown	show	VBN
cord-048471-7jszm1nd	120	7	to	to	TO
cord-048471-7jszm1nd	120	8	be	be	VB
cord-048471-7jszm1nd	120	9	the	the	DT
cord-048471-7jszm1nd	120	10	leading	leading	JJ
cord-048471-7jszm1nd	120	11	cause	cause	NN
cord-048471-7jszm1nd	120	12	of	of	IN
cord-048471-7jszm1nd	120	13	non	non	JJ
cord-048471-7jszm1nd	120	14	-	-	JJ
cord-048471-7jszm1nd	120	15	bacterial	bacterial	JJ
cord-048471-7jszm1nd	120	16	gastroenteritis	gastroenteritis	NN
cord-048471-7jszm1nd	120	17	[	[	-LRB-
cord-048471-7jszm1nd	120	18	31	31	CD
cord-048471-7jszm1nd	120	19	]	]	-RRB-
cord-048471-7jszm1nd	120	20	,	,	,
cord-048471-7jszm1nd	120	21	however	however	WRB
cord-048471-7jszm1nd	120	22	there	there	EX
cord-048471-7jszm1nd	120	23	is	be	VBZ
cord-048471-7jszm1nd	120	24	currently	currently	RB
cord-048471-7jszm1nd	121	1	no	no	DT
cord-048471-7jszm1nd	121	2	cell	cell	NN
cord-048471-7jszm1nd	121	3	culture	culture	NN
cord-048471-7jszm1nd	121	4	system	system	NN
cord-048471-7jszm1nd	121	5	available	available	JJ
cord-048471-7jszm1nd	121	6	to	to	TO
cord-048471-7jszm1nd	121	7	facilitate	facilitate	VB
cord-048471-7jszm1nd	121	8	viral	viral	JJ
cord-048471-7jszm1nd	121	9	replication	replication	NN
cord-048471-7jszm1nd	121	10	and	and	CC
cord-048471-7jszm1nd	121	11	ethical	ethical	JJ
cord-048471-7jszm1nd	121	12	considerations	consideration	NNS
cord-048471-7jszm1nd	121	13	have	have	VBP
cord-048471-7jszm1nd	121	14	hindered	hinder	VBN
cord-048471-7jszm1nd	121	15	progress	progress	NN
cord-048471-7jszm1nd	121	16	in	in	IN
cord-048471-7jszm1nd	121	17	establishing	establish	VBG
cord-048471-7jszm1nd	121	18	a	a	DT
cord-048471-7jszm1nd	121	19	permissive	permissive	JJ
cord-048471-7jszm1nd	121	20	human	human	JJ
cord-048471-7jszm1nd	121	21	organ	organ	NN
cord-048471-7jszm1nd	121	22	culture	culture	NN
cord-048471-7jszm1nd	121	23	system	system	NN
cord-048471-7jszm1nd	121	24	.	.	.
cord-048471-7jszm1nd	122	1	The	the	DT
cord-048471-7jszm1nd	122	2	study	study	NN
cord-048471-7jszm1nd	122	3	of	of	IN
cord-048471-7jszm1nd	122	4	Jena	Jena	NNP
cord-048471-7jszm1nd	122	5	virus	virus	NN
cord-048471-7jszm1nd	122	6	offers	offer	VBZ
cord-048471-7jszm1nd	122	7	a	a	DT
cord-048471-7jszm1nd	122	8	potential	potential	JJ
cord-048471-7jszm1nd	122	9	animal	animal	NN
cord-048471-7jszm1nd	122	10	model	model	NN
cord-048471-7jszm1nd	122	11	of	of	IN
cord-048471-7jszm1nd	122	12	enteric	enteric	JJ
cord-048471-7jszm1nd	122	13	noroviral	noroviral	JJ
cord-048471-7jszm1nd	122	14	infection	infection	NN
cord-048471-7jszm1nd	122	15	.	.	.
cord-048471-7jszm1nd	123	1	However	however	RB
cord-048471-7jszm1nd	123	2	,	,	,
cord-048471-7jszm1nd	123	3	until	until	IN
cord-048471-7jszm1nd	123	4	a	a	DT
cord-048471-7jszm1nd	123	5	permissive	permissive	JJ
cord-048471-7jszm1nd	123	6	bovine	bovine	JJ
cord-048471-7jszm1nd	123	7	cell	cell	NN
cord-048471-7jszm1nd	123	8	and/or	and/or	CC
cord-048471-7jszm1nd	123	9	organ	organ	NN
cord-048471-7jszm1nd	123	10	culture	culture	NN
cord-048471-7jszm1nd	123	11	systems	system	NNS
cord-048471-7jszm1nd	123	12	is	be	VBZ
cord-048471-7jszm1nd	123	13	established	establish	VBN
cord-048471-7jszm1nd	123	14	analysis	analysis	NN
cord-048471-7jszm1nd	123	15	of	of	IN
cord-048471-7jszm1nd	123	16	the	the	DT
cord-048471-7jszm1nd	123	17	molecular	molecular	JJ
cord-048471-7jszm1nd	123	18	mechanisms	mechanism	NNS
cord-048471-7jszm1nd	123	19	underpinning	underpin	VBG
cord-048471-7jszm1nd	123	20	viral	viral	JJ
cord-048471-7jszm1nd	123	21	replication	replication	NN
cord-048471-7jszm1nd	123	22	and	and	CC
cord-048471-7jszm1nd	123	23	pathogenesis	pathogenesis	NN
cord-048471-7jszm1nd	123	24	rely	rely	VBP
cord-048471-7jszm1nd	123	25	upon	upon	IN
cord-048471-7jszm1nd	123	26	in	in	FW
cord-048471-7jszm1nd	123	27	vitro	vitro	FW
cord-048471-7jszm1nd	123	28	systems	system	NNS
cord-048471-7jszm1nd	123	29	,	,	,
cord-048471-7jszm1nd	123	30	most	most	RBS
cord-048471-7jszm1nd	123	31	notably	notably	RB
cord-048471-7jszm1nd	123	32	polyprotein	polyprotein	JJ
cord-048471-7jszm1nd	123	33	synthesis	synthesis	NN
cord-048471-7jszm1nd	123	34	and	and	CC
cord-048471-7jszm1nd	123	35	processing	processing	NN
cord-048471-7jszm1nd	123	36	.	.	.
cord-048471-7jszm1nd	124	1	Unlike	unlike	IN
cord-048471-7jszm1nd	124	2	similarly	similarly	RB
cord-048471-7jszm1nd	124	3	studied	study	VBN
cord-048471-7jszm1nd	124	4	human	human	JJ
cord-048471-7jszm1nd	124	5	noroviruses	noroviruse	NNS
cord-048471-7jszm1nd	124	6	JV	JV	NNP
cord-048471-7jszm1nd	124	7	initially	initially	RB
cord-048471-7jszm1nd	124	8	did	do	VBD
cord-048471-7jszm1nd	124	9	not	not	RB
cord-048471-7jszm1nd	124	10	appear	appear	VB
cord-048471-7jszm1nd	124	11	to	to	TO
cord-048471-7jszm1nd	124	12	express	express	VB
cord-048471-7jszm1nd	124	13	the	the	DT
cord-048471-7jszm1nd	124	14	N	n	CD
cord-048471-7jszm1nd	124	15	-	-	HYPH
cord-048471-7jszm1nd	124	16	terminal	terminal	NN
cord-048471-7jszm1nd	124	17	protein	protein	NN
cord-048471-7jszm1nd	124	18	,	,	,
cord-048471-7jszm1nd	124	19	presenting	present	VBG
cord-048471-7jszm1nd	124	20	the	the	DT
cord-048471-7jszm1nd	124	21	possibility	possibility	NN
cord-048471-7jszm1nd	124	22	that	that	IN
cord-048471-7jszm1nd	124	23	the	the	DT
cord-048471-7jszm1nd	124	24	encoding	encoding	NN
cord-048471-7jszm1nd	124	25	RNA	RNA	NNP
cord-048471-7jszm1nd	124	26	sequence	sequence	NN
cord-048471-7jszm1nd	124	27	had	have	VBD
cord-048471-7jszm1nd	124	28	a	a	DT
cord-048471-7jszm1nd	124	29	regulatory	regulatory	JJ
cord-048471-7jszm1nd	124	30	function	function	NN
cord-048471-7jszm1nd	124	31	itself	-PRON-	PRP
cord-048471-7jszm1nd	124	32	,	,	,
cord-048471-7jszm1nd	124	33	most	most	RBS
cord-048471-7jszm1nd	124	34	likely	likely	RB
cord-048471-7jszm1nd	124	35	involved	involve	VBN
cord-048471-7jszm1nd	124	36	in	in	IN
cord-048471-7jszm1nd	124	37	translation	translation	NN
cord-048471-7jszm1nd	124	38	initiation	initiation	NN
cord-048471-7jszm1nd	124	39	in	in	IN
cord-048471-7jszm1nd	124	40	an	an	DT
cord-048471-7jszm1nd	124	41	IRES	IRES	NNP
cord-048471-7jszm1nd	124	42	-	-	HYPH
cord-048471-7jszm1nd	124	43	like	like	JJ
cord-048471-7jszm1nd	124	44	manner	manner	NN
cord-048471-7jszm1nd	124	45	.	.	.
cord-048471-7jszm1nd	125	1	This	this	DT
cord-048471-7jszm1nd	125	2	was	be	VBD
cord-048471-7jszm1nd	125	3	shown	show	VBN
cord-048471-7jszm1nd	125	4	not	not	RB
cord-048471-7jszm1nd	125	5	to	to	TO
cord-048471-7jszm1nd	125	6	be	be	VB
cord-048471-7jszm1nd	125	7	the	the	DT
cord-048471-7jszm1nd	125	8	case	case	NN
cord-048471-7jszm1nd	125	9	and	and	CC
cord-048471-7jszm1nd	125	10	,	,	,
cord-048471-7jszm1nd	125	11	following	follow	VBG
cord-048471-7jszm1nd	125	12	determination	determination	NN
cord-048471-7jszm1nd	125	13	of	of	IN
cord-048471-7jszm1nd	125	14	the	the	DT
cord-048471-7jszm1nd	125	15	site	site	NN
cord-048471-7jszm1nd	125	16	of	of	IN
cord-048471-7jszm1nd	125	17	translation	translation	NN
cord-048471-7jszm1nd	125	18	initiation	initiation	NN
cord-048471-7jszm1nd	125	19	at	at	IN
cord-048471-7jszm1nd	125	20	the	the	DT
cord-048471-7jszm1nd	125	21	predicted	predict	VBN
cord-048471-7jszm1nd	125	22	nucleotide	nucleotide	JJ
cord-048471-7jszm1nd	125	23	22	22	CD
cord-048471-7jszm1nd	125	24	the	the	DT
cord-048471-7jszm1nd	125	25	N	n	CD
cord-048471-7jszm1nd	125	26	-	-	HYPH
cord-048471-7jszm1nd	125	27	term	term	NN
cord-048471-7jszm1nd	125	28	protein	protein	NN
cord-048471-7jszm1nd	125	29	was	be	VBD
cord-048471-7jszm1nd	125	30	detected	detect	VBN
cord-048471-7jszm1nd	125	31	following	follow	VBG
cord-048471-7jszm1nd	125	32	the	the	DT
cord-048471-7jszm1nd	125	33	insertion	insertion	NN
cord-048471-7jszm1nd	125	34	of	of	IN
cord-048471-7jszm1nd	125	35	an	an	DT
cord-048471-7jszm1nd	125	36	epitope	epitope	NN
cord-048471-7jszm1nd	125	37	tag	tag	NN
cord-048471-7jszm1nd	125	38	,	,	,
cord-048471-7jszm1nd	125	39	both	both	CC
cord-048471-7jszm1nd	125	40	in	in	IN
cord-048471-7jszm1nd	125	41	vitro	vitro	FW
cord-048471-7jszm1nd	125	42	and	and	CC
cord-048471-7jszm1nd	125	43	in	in	IN
cord-048471-7jszm1nd	125	44	vivo	vivo	NNP
cord-048471-7jszm1nd	125	45	.	.	.
cord-048471-7jszm1nd	126	1	Although	although	IN
cord-048471-7jszm1nd	126	2	slightly	slightly	RB
cord-048471-7jszm1nd	126	3	larger	large	JJR
cord-048471-7jszm1nd	126	4	than	than	IN
cord-048471-7jszm1nd	126	5	predicted	predict	VBD
cord-048471-7jszm1nd	126	6	the	the	DT
cord-048471-7jszm1nd	126	7	N	n	CD
cord-048471-7jszm1nd	126	8	-	-	HYPH
cord-048471-7jszm1nd	126	9	term	term	NN
cord-048471-7jszm1nd	126	10	protein	protein	NN
cord-048471-7jszm1nd	126	11	was	be	VBD
cord-048471-7jszm1nd	126	12	detected	detect	VBN
cord-048471-7jszm1nd	126	13	in	in	IN
cord-048471-7jszm1nd	126	14	a	a	DT
cord-048471-7jszm1nd	126	15	processed	process	VBN
cord-048471-7jszm1nd	126	16	form	form	NN
cord-048471-7jszm1nd	126	17	in	in	IN
cord-048471-7jszm1nd	126	18	vivo	vivo	NN
cord-048471-7jszm1nd	126	19	,	,	,
cord-048471-7jszm1nd	126	20	thus	thus	RB
cord-048471-7jszm1nd	126	21	not	not	RB
cord-048471-7jszm1nd	126	22	only	only	RB
cord-048471-7jszm1nd	126	23	demonstrating	demonstrate	VBG
cord-048471-7jszm1nd	126	24	initial	initial	JJ
cord-048471-7jszm1nd	126	25	translation	translation	NN
cord-048471-7jszm1nd	126	26	of	of	IN
cord-048471-7jszm1nd	126	27	the	the	DT
cord-048471-7jszm1nd	126	28	ORF1	ORF1	NNP
cord-048471-7jszm1nd	126	29	polyprotein	polyprotein	NN
cord-048471-7jszm1nd	126	30	but	but	CC
cord-048471-7jszm1nd	126	31	also	also	RB
cord-048471-7jszm1nd	126	32	activity	activity	NN
cord-048471-7jszm1nd	126	33	of	of	IN
cord-048471-7jszm1nd	126	34	the	the	DT
cord-048471-7jszm1nd	126	35	viral	viral	JJ
cord-048471-7jszm1nd	126	36	encoded	encode	VBN
cord-048471-7jszm1nd	126	37	protease	protease	NN
cord-048471-7jszm1nd	126	38	.	.	.
cord-048471-7jszm1nd	127	1	These	these	DT
cord-048471-7jszm1nd	127	2	important	important	JJ
cord-048471-7jszm1nd	127	3	findings	finding	NNS
cord-048471-7jszm1nd	127	4	indicate	indicate	VBP
cord-048471-7jszm1nd	127	5	that	that	IN
cord-048471-7jszm1nd	127	6	the	the	DT
cord-048471-7jszm1nd	127	7	block	block	NN
cord-048471-7jszm1nd	127	8	to	to	IN
cord-048471-7jszm1nd	127	9	replication	replication	NN
cord-048471-7jszm1nd	127	10	of	of	IN
cord-048471-7jszm1nd	127	11	enteric	enteric	JJ
cord-048471-7jszm1nd	127	12	norovirus	norovirus	NN
cord-048471-7jszm1nd	127	13	in	in	IN
cord-048471-7jszm1nd	127	14	cultured	cultured	JJ
cord-048471-7jszm1nd	127	15	cells	cell	NNS
cord-048471-7jszm1nd	127	16	can	can	MD
cord-048471-7jszm1nd	127	17	not	not	RB
cord-048471-7jszm1nd	127	18	be	be	VB
cord-048471-7jszm1nd	127	19	attributed	attribute	VBN
cord-048471-7jszm1nd	127	20	to	to	IN
cord-048471-7jszm1nd	127	21	a	a	DT
cord-048471-7jszm1nd	127	22	failure	failure	NN
cord-048471-7jszm1nd	127	23	to	to	TO
cord-048471-7jszm1nd	127	24	synthesise	synthesise	VB
cord-048471-7jszm1nd	127	25	and	and	CC
cord-048471-7jszm1nd	127	26	process	process	VB
cord-048471-7jszm1nd	127	27	the	the	DT
cord-048471-7jszm1nd	127	28	non	non	JJ
cord-048471-7jszm1nd	127	29	-	-	JJ
cord-048471-7jszm1nd	127	30	structural	structural	JJ
cord-048471-7jszm1nd	127	31	proteins	protein	NNS
cord-048471-7jszm1nd	127	32	.	.	.
cord-048471-7jszm1nd	128	1	The	the	DT
cord-048471-7jszm1nd	128	2	detection	detection	NN
cord-048471-7jszm1nd	128	3	of	of	IN
cord-048471-7jszm1nd	128	4	processed	process	VBN
cord-048471-7jszm1nd	128	5	and	and	CC
cord-048471-7jszm1nd	128	6	active	active	JJ
cord-048471-7jszm1nd	128	7	ORF1	ORF1	NNP
cord-048471-7jszm1nd	128	8	proteins	protein	NNS
cord-048471-7jszm1nd	128	9	in	in	IN
cord-048471-7jszm1nd	128	10	transfected	transfecte	VBN
cord-048471-7jszm1nd	128	11	cultured	cultured	JJ
cord-048471-7jszm1nd	128	12	cells	cell	NNS
cord-048471-7jszm1nd	128	13	,	,	,
cord-048471-7jszm1nd	128	14	however	however	RB
cord-048471-7jszm1nd	128	15	,	,	,
cord-048471-7jszm1nd	128	16	highlights	highlight	VBZ
cord-048471-7jszm1nd	128	17	the	the	DT
cord-048471-7jszm1nd	128	18	potential	potential	NN
cord-048471-7jszm1nd	128	19	for	for	IN
cord-048471-7jszm1nd	128	20	the	the	DT
cord-048471-7jszm1nd	128	21	development	development	NN
cord-048471-7jszm1nd	128	22	of	of	IN
cord-048471-7jszm1nd	128	23	cell	cell	NN
cord-048471-7jszm1nd	128	24	and	and	CC
cord-048471-7jszm1nd	128	25	bovine	bovine	JJ
cord-048471-7jszm1nd	128	26	organ	organ	NN
cord-048471-7jszm1nd	128	27	based	base	VBN
cord-048471-7jszm1nd	128	28	systems	system	NNS
cord-048471-7jszm1nd	128	29	to	to	TO
cord-048471-7jszm1nd	128	30	facilitate	facilitate	VB
cord-048471-7jszm1nd	128	31	the	the	DT
cord-048471-7jszm1nd	128	32	replication	replication	NN
cord-048471-7jszm1nd	128	33	of	of	IN
cord-048471-7jszm1nd	128	34	Jena	Jena	NNP
cord-048471-7jszm1nd	128	35	virus	virus	NN
cord-048471-7jszm1nd	128	36	.	.	.
cord-048471-7jszm1nd	129	1	The	the	DT
cord-048471-7jszm1nd	129	2	pEGFP	pEGFP	NNP
cord-048471-7jszm1nd	129	3	-	-	HYPH
cord-048471-7jszm1nd	129	4	C1	c1	NN
cord-048471-7jszm1nd	129	5	vector	vector	NN
cord-048471-7jszm1nd	129	6	(	(	-LRB-
cord-048471-7jszm1nd	129	7	Clontech	Clontech	NNP
cord-048471-7jszm1nd	129	8	)	)	-RRB-
cord-048471-7jszm1nd	129	9	comprises	comprise	VBZ
cord-048471-7jszm1nd	129	10	of	of	IN
cord-048471-7jszm1nd	129	11	an	an	DT
cord-048471-7jszm1nd	129	12	EGFP	EGFP	NNP
cord-048471-7jszm1nd	129	13	coding	coding	NN
cord-048471-7jszm1nd	129	14	sequence	sequence	NN
cord-048471-7jszm1nd	129	15	under	under	IN
cord-048471-7jszm1nd	129	16	the	the	DT
cord-048471-7jszm1nd	129	17	control	control	NN
cord-048471-7jszm1nd	129	18	of	of	IN
cord-048471-7jszm1nd	129	19	a	a	DT
cord-048471-7jszm1nd	129	20	CMV	CMV	NNP
cord-048471-7jszm1nd	129	21	promoter	promoter	NN
cord-048471-7jszm1nd	129	22	and	and	CC
cord-048471-7jszm1nd	129	23	a	a	DT
cord-048471-7jszm1nd	129	24	Kozak	Kozak	NNP
cord-048471-7jszm1nd	129	25	translation	translation	NN
cord-048471-7jszm1nd	129	26	initiation	initiation	NN
cord-048471-7jszm1nd	129	27	site	site	NN
cord-048471-7jszm1nd	129	28	.	.	.
cord-048471-7jszm1nd	130	1	Downstream	downstream	IN
cord-048471-7jszm1nd	130	2	of	of	IN
cord-048471-7jszm1nd	130	3	the	the	DT
cord-048471-7jszm1nd	130	4	EGFP	EGFP	NNP
cord-048471-7jszm1nd	130	5	sequence	sequence	NN
cord-048471-7jszm1nd	130	6	is	be	VBZ
cord-048471-7jszm1nd	130	7	the	the	DT
cord-048471-7jszm1nd	130	8	multiple	multiple	JJ
cord-048471-7jszm1nd	130	9	cloning	cloning	NN
cord-048471-7jszm1nd	130	10	site	site	NN
cord-048471-7jszm1nd	130	11	containing	contain	VBG
cord-048471-7jszm1nd	130	12	unique	unique	JJ
cord-048471-7jszm1nd	130	13	BglII	bglii	NN
cord-048471-7jszm1nd	130	14	,	,	,
cord-048471-7jszm1nd	130	15	SacI	SacI	NNP
cord-048471-7jszm1nd	130	16	,	,	,
cord-048471-7jszm1nd	130	17	HindIII	HindIII	NNP
cord-048471-7jszm1nd	130	18	and	and	CC
cord-048471-7jszm1nd	130	19	ApaI	ApaI	NNP
cord-048471-7jszm1nd	130	20	restriction	restriction	NN
cord-048471-7jszm1nd	130	21	sites	site	NNS
cord-048471-7jszm1nd	130	22	.	.	.
cord-048471-7jszm1nd	131	1	Contruction	Contruction	NNP
cord-048471-7jszm1nd	131	2	of	of	IN
cord-048471-7jszm1nd	131	3	pEGFP	pEGFP	NNP
cord-048471-7jszm1nd	131	4	-	-	HYPH
cord-048471-7jszm1nd	131	5	C1	C1	NNP
cord-048471-7jszm1nd	131	6	/	/	SYM
cord-048471-7jszm1nd	131	7	JV	JV	NNP
cord-048471-7jszm1nd	131	8	59	59	CD
cord-048471-7jszm1nd	131	9	GS	GS	NNP
cord-048471-7jszm1nd	131	10	/	/	SYM
cord-048471-7jszm1nd	131	11	lacZ	lacZ	NNP
cord-048471-7jszm1nd	131	12	was	be	VBD
cord-048471-7jszm1nd	131	13	as	as	IN
cord-048471-7jszm1nd	131	14	follows	follow	VBZ
cord-048471-7jszm1nd	131	15	;	;	:
cord-048471-7jszm1nd	131	16	The	the	DT
cord-048471-7jszm1nd	131	17	JV	JV	NNP
cord-048471-7jszm1nd	131	18	59	59	CD
cord-048471-7jszm1nd	131	19	GS	GS	NNP
cord-048471-7jszm1nd	131	20	sequence	sequence	NN
cord-048471-7jszm1nd	131	21	was	be	VBD
cord-048471-7jszm1nd	131	22	amplified	amplify	VBN
cord-048471-7jszm1nd	131	23	from	from	IN
cord-048471-7jszm1nd	131	24	the	the	DT
cord-048471-7jszm1nd	131	25	JV	JV	NNP
cord-048471-7jszm1nd	131	26	full	full	JJ
cord-048471-7jszm1nd	131	27	length	length	NN
cord-048471-7jszm1nd	131	28	cDNA	cdna	NN
cord-048471-7jszm1nd	131	29	clone	clone	NN
cord-048471-7jszm1nd	131	30	[	[	-LRB-
cord-048471-7jszm1nd	131	31	7	7	CD
cord-048471-7jszm1nd	131	32	]	]	-RRB-
cord-048471-7jszm1nd	131	33	using	use	VBG
cord-048471-7jszm1nd	131	34	Bio	Bio	NNP
cord-048471-7jszm1nd	131	35	-	-	HYPH
cord-048471-7jszm1nd	131	36	X	X	NNP
cord-048471-7jszm1nd	131	37	-	-	HYPH
cord-048471-7jszm1nd	131	38	Act	act	NN
cord-048471-7jszm1nd	131	39	DNA	dna	NN
cord-048471-7jszm1nd	131	40	polymerase	polymerase	NN
cord-048471-7jszm1nd	131	41	(	(	-LRB-
cord-048471-7jszm1nd	131	42	Bioline	Bioline	NNP
cord-048471-7jszm1nd	131	43	)	)	-RRB-
cord-048471-7jszm1nd	131	44	with	with	IN
cord-048471-7jszm1nd	131	45	the	the	DT
cord-048471-7jszm1nd	131	46	primers	primer	NNS
cord-048471-7jszm1nd	131	47	59	59	CD
cord-048471-7jszm1nd	131	48	GS	GS	NNP
cord-048471-7jszm1nd	131	49	F	F	NNP
cord-048471-7jszm1nd	131	50	(	(	-LRB-
cord-048471-7jszm1nd	131	51	59-AACTGCA	59-aactgca	CD
cord-048471-7jszm1nd	131	52	-	-	HYPH
cord-048471-7jszm1nd	131	53	GATCTTAATAAGTGAATGAAGACTTTGACGAT-39	gatcttaataagtgaatgaagactttgacgat-39	NN
cord-048471-7jszm1nd	131	54	)	)	-RRB-
cord-048471-7jszm1nd	131	55	,	,	,
cord-048471-7jszm1nd	131	56	containing	contain	VBG
cord-048471-7jszm1nd	131	57	the	the	DT
cord-048471-7jszm1nd	131	58	BglII	BglII	NNP
cord-048471-7jszm1nd	131	59	restriction	restriction	NN
cord-048471-7jszm1nd	131	60	site	site	NN
cord-048471-7jszm1nd	131	61	(	(	-LRB-
cord-048471-7jszm1nd	131	62	bold	bold	JJ
cord-048471-7jszm1nd	131	63	)	)	-RRB-
cord-048471-7jszm1nd	131	64	and	and	CC
cord-048471-7jszm1nd	131	65	two	two	CD
cord-048471-7jszm1nd	131	66	in	in	IN
cord-048471-7jszm1nd	131	67	-	-	HYPH
cord-048471-7jszm1nd	131	68	frame	frame	NN
cord-048471-7jszm1nd	131	69	translation	translation	NN
cord-048471-7jszm1nd	131	70	termination	termination	NN
cord-048471-7jszm1nd	131	71	codons	codon	NNS
cord-048471-7jszm1nd	131	72	(	(	-LRB-
cord-048471-7jszm1nd	131	73	underlined	underline	VBN
cord-048471-7jszm1nd	131	74	)	)	-RRB-
cord-048471-7jszm1nd	131	75	to	to	TO
cord-048471-7jszm1nd	131	76	ensure	ensure	VB
cord-048471-7jszm1nd	131	77	that	that	DT
cord-048471-7jszm1nd	131	78	translation	translation	NN
cord-048471-7jszm1nd	131	79	of	of	IN
cord-048471-7jszm1nd	131	80	the	the	DT
cord-048471-7jszm1nd	131	81	EGFP	EGFP	NNP
cord-048471-7jszm1nd	131	82	sequence	sequence	NN
cord-048471-7jszm1nd	131	83	did	do	VBD
cord-048471-7jszm1nd	131	84	not	not	RB
cord-048471-7jszm1nd	131	85	carry	carry	VB
cord-048471-7jszm1nd	131	86	over	over	RP
cord-048471-7jszm1nd	131	87	to	to	IN
cord-048471-7jszm1nd	131	88	the	the	DT
cord-048471-7jszm1nd	131	89	59	59	CD
cord-048471-7jszm1nd	131	90	GS	GS	NNP
cord-048471-7jszm1nd	131	91	,	,	,
cord-048471-7jszm1nd	131	92	and	and	CC
cord-048471-7jszm1nd	131	93	59	59	CD
cord-048471-7jszm1nd	131	94	GS	gs	NN
cord-048471-7jszm1nd	131	95	R	r	NN
cord-048471-7jszm1nd	131	96	(	(	-LRB-
cord-048471-7jszm1nd	131	97	59-AACTGCAAGCTTCTGCAGGACACAATGAGG-39	59-AACTGCAAGCTTCTGCAGGACACAATGAGG-39	NNP
cord-048471-7jszm1nd	131	98	)	)	-RRB-
cord-048471-7jszm1nd	131	99	,	,	,
cord-048471-7jszm1nd	131	100	containing	contain	VBG
cord-048471-7jszm1nd	131	101	theHindIII	thehindiii	CD
cord-048471-7jszm1nd	131	102	restriction	restriction	NN
cord-048471-7jszm1nd	131	103	site	site	NN
cord-048471-7jszm1nd	131	104	.	.	.
cord-048471-7jszm1nd	132	1	The	the	DT
cord-048471-7jszm1nd	132	2	JV	JV	NNP
cord-048471-7jszm1nd	132	3	59	59	CD
cord-048471-7jszm1nd	132	4	GS	GS	NNP
cord-048471-7jszm1nd	132	5	amplicon	amplicon	NN
cord-048471-7jszm1nd	132	6	was	be	VBD
cord-048471-7jszm1nd	132	7	ligated	ligate	VBN
cord-048471-7jszm1nd	132	8	to	to	IN
cord-048471-7jszm1nd	132	9	the	the	DT
cord-048471-7jszm1nd	132	10	pEGFP	pEGFP	NNP
cord-048471-7jszm1nd	132	11	-	-	HYPH
cord-048471-7jszm1nd	132	12	C1	c1	NN
cord-048471-7jszm1nd	132	13	vector	vector	NN
cord-048471-7jszm1nd	132	14	,	,	,
cord-048471-7jszm1nd	132	15	following	follow	VBG
cord-048471-7jszm1nd	132	16	restriction	restriction	NN
cord-048471-7jszm1nd	132	17	enzyme	enzyme	NN
cord-048471-7jszm1nd	132	18	digestion	digestion	NN
cord-048471-7jszm1nd	132	19	of	of	IN
cord-048471-7jszm1nd	132	20	both	both	DT
cord-048471-7jszm1nd	132	21	amplicon	amplicon	NN
cord-048471-7jszm1nd	132	22	and	and	CC
cord-048471-7jszm1nd	132	23	vector	vector	NN
cord-048471-7jszm1nd	132	24	with	with	IN
cord-048471-7jszm1nd	132	25	BglII	bglii	NN
cord-048471-7jszm1nd	132	26	and	and	CC
cord-048471-7jszm1nd	132	27	HindIII	hindiii	NN
cord-048471-7jszm1nd	132	28	restriction	restriction	NN
cord-048471-7jszm1nd	132	29	enzymes	enzyme	NNS
cord-048471-7jszm1nd	132	30	,	,	,
cord-048471-7jszm1nd	132	31	and	and	CC
cord-048471-7jszm1nd	132	32	the	the	DT
cord-048471-7jszm1nd	132	33	ligated	ligate	VBN
cord-048471-7jszm1nd	132	34	DNA	dna	NN
cord-048471-7jszm1nd	132	35	used	use	VBN
cord-048471-7jszm1nd	132	36	to	to	TO
cord-048471-7jszm1nd	132	37	transform	transform	VB
cord-048471-7jszm1nd	132	38	E.coli	e.coli	JJ
cord-048471-7jszm1nd	132	39	Top10	Top10	NNP
cord-048471-7jszm1nd	132	40	(	(	-LRB-
cord-048471-7jszm1nd	132	41	Invitrogen	Invitrogen	NNP
cord-048471-7jszm1nd	132	42	)	)	-RRB-
cord-048471-7jszm1nd	132	43	.	.	.
cord-048471-7jszm1nd	133	1	This	this	DT
cord-048471-7jszm1nd	133	2	intermediate	intermediate	JJ
cord-048471-7jszm1nd	133	3	construct	construct	NN
cord-048471-7jszm1nd	133	4	was	be	VBD
cord-048471-7jszm1nd	133	5	named	name	VBN
cord-048471-7jszm1nd	133	6	pEGFP	pEGFP	NNP
cord-048471-7jszm1nd	133	7	-	-	HYPH
cord-048471-7jszm1nd	133	8	C1/	C1/	NNP
cord-048471-7jszm1nd	133	9	JV	JV	NNP
cord-048471-7jszm1nd	133	10	59	59	CD
cord-048471-7jszm1nd	133	11	GS	GS	NNP
cord-048471-7jszm1nd	133	12	.	.	.
cord-048471-7jszm1nd	134	1	The	the	DT
cord-048471-7jszm1nd	134	2	lacZ	lacZ	NNP
cord-048471-7jszm1nd	134	3	coding	code	VBG
cord-048471-7jszm1nd	134	4	sequence	sequence	NN
cord-048471-7jszm1nd	134	5	was	be	VBD
cord-048471-7jszm1nd	134	6	amplified	amplify	VBN
cord-048471-7jszm1nd	134	7	from	from	IN
cord-048471-7jszm1nd	134	8	the	the	DT
cord-048471-7jszm1nd	134	9	pSVb	psvb	JJ
cord-048471-7jszm1nd	134	10	-	-	HYPH
cord-048471-7jszm1nd	134	11	Gal	Gal	NNP
cord-048471-7jszm1nd	134	12	reporter	reporter	NN
cord-048471-7jszm1nd	134	13	vector	vector	NN
cord-048471-7jszm1nd	134	14	(	(	-LRB-
cord-048471-7jszm1nd	134	15	Promega	Promega	NNP
cord-048471-7jszm1nd	134	16	)	)	-RRB-
cord-048471-7jszm1nd	134	17	using	use	VBG
cord-048471-7jszm1nd	134	18	Bio	Bio	NNP
cord-048471-7jszm1nd	134	19	-	-	HYPH
cord-048471-7jszm1nd	134	20	X	X	NNP
cord-048471-7jszm1nd	134	21	-	-	HYPH
cord-048471-7jszm1nd	134	22	Act	act	NN
cord-048471-7jszm1nd	134	23	DNA	dna	NN
cord-048471-7jszm1nd	134	24	polymerase	polymerase	NN
cord-048471-7jszm1nd	134	25	and	and	CC
cord-048471-7jszm1nd	134	26	the	the	DT
cord-048471-7jszm1nd	134	27	primers	primer	NNS
cord-048471-7jszm1nd	134	28	lacZ	lacz	XX
cord-048471-7jszm1nd	135	1	F	F	NNP
cord-048471-7jszm1nd	135	2	(	(	-LRB-
cord-048471-7jszm1nd	135	3	59-AACTGCAAGCTTGA	59-AACTGCAAGCTTGA	NNP
cord-048471-7jszm1nd	135	4	-	-	HYPH
cord-048471-7jszm1nd	135	5	TATGGGGGATCCCGTCGTTTTACAACG-39	tatgggggatcccgtcgttttacaacg-39	CD
cord-048471-7jszm1nd	135	6	)	)	-RRB-
cord-048471-7jszm1nd	135	7	,	,	,
cord-048471-7jszm1nd	135	8	containing	contain	VBG
cord-048471-7jszm1nd	135	9	the	the	DT
cord-048471-7jszm1nd	135	10	HindIII	HindIII	NNP
cord-048471-7jszm1nd	135	11	restriction	restriction	NN
cord-048471-7jszm1nd	135	12	site	site	NN
cord-048471-7jszm1nd	135	13	(	(	-LRB-
cord-048471-7jszm1nd	135	14	bold	bold	JJ
cord-048471-7jszm1nd	135	15	)	)	-RRB-
cord-048471-7jszm1nd	135	16	and	and	CC
cord-048471-7jszm1nd	135	17	a	a	DT
cord-048471-7jszm1nd	135	18	kozak	kozak	NNP
cord-048471-7jszm1nd	135	19	translation	translation	NN
cord-048471-7jszm1nd	135	20	initiation	initiation	NN
cord-048471-7jszm1nd	135	21	site	site	NN
cord-048471-7jszm1nd	135	22	(	(	-LRB-
cord-048471-7jszm1nd	135	23	underlined	underline	VBN
cord-048471-7jszm1nd	135	24	)	)	-RRB-
cord-048471-7jszm1nd	135	25	,	,	,
cord-048471-7jszm1nd	135	26	and	and	CC
cord-048471-7jszm1nd	135	27	lacZ	lacZ	NNP
cord-048471-7jszm1nd	135	28	R	r	NN
cord-048471-7jszm1nd	135	29	(	(	-LRB-
cord-048471-7jszm1nd	135	30	59-AACTGCGGGCCCTTAT	59-aactgcgggcccttat	CD
cord-048471-7jszm1nd	135	31	-	-	HYPH
cord-048471-7jszm1nd	135	32	TATTTTTGACACCAGACCA-39	tatttttgacaccagacca-39	NN
cord-048471-7jszm1nd	135	33	)	)	-RRB-
cord-048471-7jszm1nd	135	34	containing	contain	VBG
cord-048471-7jszm1nd	135	35	the	the	DT
cord-048471-7jszm1nd	135	36	ApaI	ApaI	NNP
cord-048471-7jszm1nd	135	37	restriction	restriction	NN
cord-048471-7jszm1nd	135	38	site	site	NN
cord-048471-7jszm1nd	135	39	(	(	-LRB-
cord-048471-7jszm1nd	135	40	bold	bold	JJ
cord-048471-7jszm1nd	135	41	)	)	-RRB-
cord-048471-7jszm1nd	135	42	and	and	CC
cord-048471-7jszm1nd	135	43	translation	translation	NN
cord-048471-7jszm1nd	135	44	termination	termination	NN
cord-048471-7jszm1nd	135	45	codons	codon	NNS
cord-048471-7jszm1nd	135	46	(	(	-LRB-
cord-048471-7jszm1nd	135	47	underlined	underline	VBN
cord-048471-7jszm1nd	135	48	)	)	-RRB-
cord-048471-7jszm1nd	135	49	.	.	.
cord-048471-7jszm1nd	136	1	The	the	DT
cord-048471-7jszm1nd	136	2	lacZ	lacZ	NNP
cord-048471-7jszm1nd	136	3	amplicon	amplicon	NN
cord-048471-7jszm1nd	136	4	was	be	VBD
cord-048471-7jszm1nd	136	5	ligated	ligate	VBN
cord-048471-7jszm1nd	136	6	to	to	IN
cord-048471-7jszm1nd	136	7	the	the	DT
cord-048471-7jszm1nd	136	8	pEGFP	pEGFP	NNP
cord-048471-7jszm1nd	136	9	-	-	HYPH
cord-048471-7jszm1nd	136	10	C1	C1	NNP
cord-048471-7jszm1nd	136	11	/	/	SYM
cord-048471-7jszm1nd	136	12	JV	JV	NNP
cord-048471-7jszm1nd	136	13	59	59	CD
cord-048471-7jszm1nd	136	14	GS	GS	NNP
cord-048471-7jszm1nd	136	15	vector	vector	NN
cord-048471-7jszm1nd	136	16	following	follow	VBG
cord-048471-7jszm1nd	136	17	restriction	restriction	NN
cord-048471-7jszm1nd	136	18	enzyme	enzyme	NN
cord-048471-7jszm1nd	136	19	digest	digest	NN
cord-048471-7jszm1nd	136	20	of	of	IN
cord-048471-7jszm1nd	136	21	both	both	DT
cord-048471-7jszm1nd	136	22	amplicon	amplicon	NN
cord-048471-7jszm1nd	136	23	and	and	CC
cord-048471-7jszm1nd	136	24	vector	vector	NN
cord-048471-7jszm1nd	136	25	with	with	IN
cord-048471-7jszm1nd	136	26	HindIII	HindIII	NNS
cord-048471-7jszm1nd	136	27	and	and	CC
cord-048471-7jszm1nd	136	28	ApaI	ApaI	NNP
cord-048471-7jszm1nd	136	29	restriction	restriction	NN
cord-048471-7jszm1nd	136	30	enzymes	enzyme	NNS
cord-048471-7jszm1nd	136	31	,	,	,
cord-048471-7jszm1nd	136	32	and	and	CC
cord-048471-7jszm1nd	136	33	the	the	DT
cord-048471-7jszm1nd	136	34	ligated	ligate	VBN
cord-048471-7jszm1nd	136	35	DNA	dna	NN
cord-048471-7jszm1nd	136	36	used	use	VBN
cord-048471-7jszm1nd	136	37	to	to	TO
cord-048471-7jszm1nd	136	38	transform	transform	VB
cord-048471-7jszm1nd	136	39	E.coli	E.coli	NNS
cord-048471-7jszm1nd	136	40	Top10	Top10	NNP
cord-048471-7jszm1nd	136	41	.	.	.
cord-048471-7jszm1nd	137	1	The	the	DT
cord-048471-7jszm1nd	137	2	construct	construct	NN
cord-048471-7jszm1nd	137	3	was	be	VBD
cord-048471-7jszm1nd	137	4	verified	verify	VBN
cord-048471-7jszm1nd	137	5	by	by	IN
cord-048471-7jszm1nd	137	6	sequencing	sequence	VBG
cord-048471-7jszm1nd	137	7	.	.	.
cord-048471-7jszm1nd	138	1	Construction	construction	NN
cord-048471-7jszm1nd	138	2	of	of	IN
cord-048471-7jszm1nd	138	3	pEGFP	pEGFP	NNP
cord-048471-7jszm1nd	138	4	-	-	HYPH
cord-048471-7jszm1nd	138	5	C1	C1	NNP
cord-048471-7jszm1nd	138	6	/	/	SYM
cord-048471-7jszm1nd	138	7	IRES	IRES	NNP
cord-048471-7jszm1nd	138	8	/	/	SYM
cord-048471-7jszm1nd	138	9	lacZ	lacZ	NNP
cord-048471-7jszm1nd	138	10	was	be	VBD
cord-048471-7jszm1nd	138	11	as	as	IN
cord-048471-7jszm1nd	138	12	follows	follow	VBZ
cord-048471-7jszm1nd	138	13	;	;	:
cord-048471-7jszm1nd	138	14	The	the	DT
cord-048471-7jszm1nd	138	15	EMCV	EMCV	NNP
cord-048471-7jszm1nd	138	16	IRES	IRES	NNP
cord-048471-7jszm1nd	138	17	sequence	sequence	NN
cord-048471-7jszm1nd	138	18	was	be	VBD
cord-048471-7jszm1nd	138	19	amplified	amplify	VBN
cord-048471-7jszm1nd	138	20	from	from	IN
cord-048471-7jszm1nd	138	21	the	the	DT
cord-048471-7jszm1nd	138	22	pIRES2-EGFP	pires2-egfp	CD
cord-048471-7jszm1nd	138	23	vector	vector	NN
cord-048471-7jszm1nd	138	24	(	(	-LRB-
cord-048471-7jszm1nd	138	25	Clontech	Clontech	NNP
cord-048471-7jszm1nd	138	26	)	)	-RRB-
cord-048471-7jszm1nd	138	27	using	use	VBG
cord-048471-7jszm1nd	138	28	Bio	Bio	NNP
cord-048471-7jszm1nd	138	29	-	-	HYPH
cord-048471-7jszm1nd	138	30	X	X	NNP
cord-048471-7jszm1nd	138	31	-	-	HYPH
cord-048471-7jszm1nd	138	32	Act	act	NN
cord-048471-7jszm1nd	138	33	DNA	dna	NN
cord-048471-7jszm1nd	138	34	polymerase	polymerase	NN
cord-048471-7jszm1nd	138	35	and	and	CC
cord-048471-7jszm1nd	138	36	the	the	DT
cord-048471-7jszm1nd	138	37	primers	primer	NNS
cord-048471-7jszm1nd	138	38	IRES	IRES	NNP
cord-048471-7jszm1nd	138	39	Bgl	Bgl	NNP
cord-048471-7jszm1nd	138	40	F	F	NNP
cord-048471-7jszm1nd	138	41	(	(	-LRB-
cord-048471-7jszm1nd	138	42	59-ACTCGAAGATCTTAA	59-actcgaagatcttaa	CD
cord-048471-7jszm1nd	138	43	-	-	HYPH
cord-048471-7jszm1nd	138	44	TAGAGCTTCGAATTCTGCAGTCGA-39	tagagcttcgaattctgcagtcga-39	CD
cord-048471-7jszm1nd	138	45	)	)	-RRB-
cord-048471-7jszm1nd	138	46	,	,	,
cord-048471-7jszm1nd	138	47	containing	contain	VBG
cord-048471-7jszm1nd	138	48	the	the	DT
cord-048471-7jszm1nd	138	49	BglII	BglII	NNP
cord-048471-7jszm1nd	138	50	restriction	restriction	NN
cord-048471-7jszm1nd	138	51	site	site	NN
cord-048471-7jszm1nd	138	52	(	(	-LRB-
cord-048471-7jszm1nd	138	53	bold	bold	JJ
cord-048471-7jszm1nd	138	54	)	)	-RRB-
cord-048471-7jszm1nd	138	55	and	and	CC
cord-048471-7jszm1nd	138	56	translation	translation	NN
cord-048471-7jszm1nd	138	57	termination	termination	NN
cord-048471-7jszm1nd	138	58	codons	codon	NNS
cord-048471-7jszm1nd	138	59	(	(	-LRB-
cord-048471-7jszm1nd	138	60	underlined	underline	VBN
cord-048471-7jszm1nd	138	61	)	)	-RRB-
cord-048471-7jszm1nd	138	62	to	to	TO
cord-048471-7jszm1nd	138	63	prevent	prevent	VB
cord-048471-7jszm1nd	138	64	carry	carry	NN
cord-048471-7jszm1nd	138	65	over	over	IN
cord-048471-7jszm1nd	138	66	translation	translation	NN
cord-048471-7jszm1nd	138	67	as	as	IN
cord-048471-7jszm1nd	138	68	before	before	RB
cord-048471-7jszm1nd	138	69	,	,	,
cord-048471-7jszm1nd	138	70	and	and	CC
cord-048471-7jszm1nd	138	71	IRES	IRES	NNP
cord-048471-7jszm1nd	138	72	Sac	Sac	NNP
cord-048471-7jszm1nd	138	73	R	r	NN
cord-048471-7jszm1nd	138	74	(	(	-LRB-
cord-048471-7jszm1nd	138	75	59-ACTCGAGAGCTCTGTGGCCATATTATCATC	59-actcgagagctctgtggccatattatcatc	CD
cord-048471-7jszm1nd	138	76	-	-	HYPH
cord-048471-7jszm1nd	138	77	GTG-39	GTG-39	NNP
cord-048471-7jszm1nd	138	78	)	)	-RRB-
cord-048471-7jszm1nd	138	79	,	,	,
cord-048471-7jszm1nd	138	80	containing	contain	VBG
cord-048471-7jszm1nd	138	81	the	the	DT
cord-048471-7jszm1nd	138	82	SacI	SacI	NNP
cord-048471-7jszm1nd	138	83	restriction	restriction	NN
cord-048471-7jszm1nd	138	84	site	site	NN
cord-048471-7jszm1nd	138	85	(	(	-LRB-
cord-048471-7jszm1nd	138	86	bold	bold	JJ
cord-048471-7jszm1nd	138	87	)	)	-RRB-
cord-048471-7jszm1nd	138	88	.	.	.
cord-048471-7jszm1nd	139	1	The	the	DT
cord-048471-7jszm1nd	139	2	IRES	ires	JJ
cord-048471-7jszm1nd	139	3	amplicon	amplicon	NN
cord-048471-7jszm1nd	139	4	was	be	VBD
cord-048471-7jszm1nd	139	5	ligated	ligate	VBN
cord-048471-7jszm1nd	139	6	to	to	IN
cord-048471-7jszm1nd	139	7	the	the	DT
cord-048471-7jszm1nd	139	8	pEGFP	pEGFP	NNP
cord-048471-7jszm1nd	139	9	-	-	HYPH
cord-048471-7jszm1nd	139	10	C1	c1	NN
cord-048471-7jszm1nd	139	11	vector	vector	NN
cord-048471-7jszm1nd	139	12	follwing	follwe	VBG
cord-048471-7jszm1nd	139	13	restriction	restriction	NN
cord-048471-7jszm1nd	139	14	Whole	whole	JJ
cord-048471-7jszm1nd	139	15	cell	cell	NN
cord-048471-7jszm1nd	139	16	lysate	lysate	NN
cord-048471-7jszm1nd	139	17	was	be	VBD
cord-048471-7jszm1nd	139	18	collected	collect	VBN
cord-048471-7jszm1nd	139	19	at	at	IN
cord-048471-7jszm1nd	139	20	the	the	DT
cord-048471-7jszm1nd	139	21	following	following	JJ
cord-048471-7jszm1nd	139	22	time	time	NN
cord-048471-7jszm1nd	139	23	points	point	NNS
cord-048471-7jszm1nd	139	24	following	follow	VBG
cord-048471-7jszm1nd	139	25	CHX	CHX	NNP
cord-048471-7jszm1nd	139	26	treatment	treatment	NN
cord-048471-7jszm1nd	139	27	:	:	:
cord-048471-7jszm1nd	139	28	0	0	CD
cord-048471-7jszm1nd	139	29	hr	hr	NN
cord-048471-7jszm1nd	139	30	,	,	,
cord-048471-7jszm1nd	139	31	1	1	CD
cord-048471-7jszm1nd	139	32	hr	hr	NN
cord-048471-7jszm1nd	139	33	,	,	,
cord-048471-7jszm1nd	139	34	3	3	CD
cord-048471-7jszm1nd	139	35	hr	hr	NN
cord-048471-7jszm1nd	139	36	,	,	,
cord-048471-7jszm1nd	139	37	6	6	CD
cord-048471-7jszm1nd	139	38	hr	hr	NN
cord-048471-7jszm1nd	139	39	,	,	,
cord-048471-7jszm1nd	139	40	12	12	CD
cord-048471-7jszm1nd	139	41	hr	hr	NN
cord-048471-7jszm1nd	139	42	,	,	,
cord-048471-7jszm1nd	139	43	24	24	CD
cord-048471-7jszm1nd	139	44	hr	hr	NN
cord-048471-7jszm1nd	139	45	.	.	.
cord-048471-7jszm1nd	140	1	Bradford	Bradford	NNP
cord-048471-7jszm1nd	140	2	analysis	analysis	NN
cord-048471-7jszm1nd	140	3	was	be	VBD
cord-048471-7jszm1nd	140	4	performed	perform	VBN
cord-048471-7jszm1nd	140	5	on	on	IN
cord-048471-7jszm1nd	140	6	the	the	DT
cord-048471-7jszm1nd	140	7	lysates	lysate	NNS
cord-048471-7jszm1nd	140	8	to	to	TO
cord-048471-7jszm1nd	140	9	ensure	ensure	VB
cord-048471-7jszm1nd	140	10	equal	equal	JJ
cord-048471-7jszm1nd	140	11	loading	loading	NN
cord-048471-7jszm1nd	140	12	.	.	.
cord-048471-7jszm1nd	141	1	Following	follow	VBG
cord-048471-7jszm1nd	141	2	Western	western	JJ
cord-048471-7jszm1nd	141	3	analysis	analysis	NN
cord-048471-7jszm1nd	141	4	the	the	DT
cord-048471-7jszm1nd	141	5	ECL	ECL	NNP
cord-048471-7jszm1nd	141	6	treated	treat	VBD
cord-048471-7jszm1nd	141	7	membrane	membrane	NN
cord-048471-7jszm1nd	141	8	was	be	VBD
cord-048471-7jszm1nd	141	9	exposed	expose	VBN
cord-048471-7jszm1nd	141	10	to	to	TO
cord-048471-7jszm1nd	141	11	film	film	VB
cord-048471-7jszm1nd	141	12	for	for	IN
cord-048471-7jszm1nd	141	13	1	1	CD
cord-048471-7jszm1nd	141	14	min	min	NN
cord-048471-7jszm1nd	141	15	.	.	.
cord-048471-7jszm1nd	142	1	Molecular	molecular	JJ
cord-048471-7jszm1nd	142	2	weight	weight	NN
cord-048471-7jszm1nd	142	3	marker	marker	NN
cord-048471-7jszm1nd	142	4	is	be	VBZ
cord-048471-7jszm1nd	142	5	represented	represent	VBN
cord-048471-7jszm1nd	142	6	in	in	IN
cord-048471-7jszm1nd	142	7	lane	lane	NN
cord-048471-7jszm1nd	142	8	1	1	CD
cord-048471-7jszm1nd	142	9	.	.	.
cord-048471-7jszm1nd	143	1	doi:10.1371	doi:10.1371	NNP
cord-048471-7jszm1nd	144	1	/journal.pone.0002169.g008	/journal.pone.0002169.g008	NNP
cord-048471-7jszm1nd	144	2	enzyme	enzyme	NN
cord-048471-7jszm1nd	144	3	digest	digest	NN
cord-048471-7jszm1nd	144	4	of	of	IN
cord-048471-7jszm1nd	144	5	both	both	DT
cord-048471-7jszm1nd	144	6	amplicon	amplicon	NN
cord-048471-7jszm1nd	144	7	and	and	CC
cord-048471-7jszm1nd	144	8	vector	vector	NN
cord-048471-7jszm1nd	144	9	with	with	IN
cord-048471-7jszm1nd	144	10	BgIII	BgIII	NNP
cord-048471-7jszm1nd	144	11	and	and	CC
cord-048471-7jszm1nd	144	12	SacI	SacI	NNP
cord-048471-7jszm1nd	144	13	restriction	restriction	NN
cord-048471-7jszm1nd	144	14	enzymes	enzyme	NNS
cord-048471-7jszm1nd	144	15	,	,	,
cord-048471-7jszm1nd	144	16	and	and	CC
cord-048471-7jszm1nd	144	17	the	the	DT
cord-048471-7jszm1nd	144	18	ligated	ligate	VBN
cord-048471-7jszm1nd	144	19	DNA	dna	NN
cord-048471-7jszm1nd	144	20	used	use	VBN
cord-048471-7jszm1nd	144	21	to	to	TO
cord-048471-7jszm1nd	144	22	transform	transform	VB
cord-048471-7jszm1nd	144	23	E.coli	E.coli	NNS
cord-048471-7jszm1nd	144	24	Top10	Top10	NNP
cord-048471-7jszm1nd	144	25	.	.	.
cord-048471-7jszm1nd	145	1	The	the	DT
cord-048471-7jszm1nd	145	2	lacZ	lacZ	NNP
cord-048471-7jszm1nd	145	3	amplicon	amplicon	NN
cord-048471-7jszm1nd	145	4	described	describe	VBN
cord-048471-7jszm1nd	145	5	previously	previously	RB
cord-048471-7jszm1nd	145	6	was	be	VBD
cord-048471-7jszm1nd	145	7	ligated	ligate	VBN
cord-048471-7jszm1nd	145	8	to	to	IN
cord-048471-7jszm1nd	145	9	the	the	DT
cord-048471-7jszm1nd	145	10	intermediate	intermediate	JJ
cord-048471-7jszm1nd	145	11	pEGFP	pEGFP	NNP
cord-048471-7jszm1nd	145	12	-	-	HYPH
cord-048471-7jszm1nd	145	13	C1	C1	NNP
cord-048471-7jszm1nd	145	14	/	/	SYM
cord-048471-7jszm1nd	145	15	IRES	IRES	NNP
cord-048471-7jszm1nd	145	16	vector	vector	NN
cord-048471-7jszm1nd	145	17	following	follow	VBG
cord-048471-7jszm1nd	145	18	restriction	restriction	NN
cord-048471-7jszm1nd	145	19	enzyme	enzyme	NN
cord-048471-7jszm1nd	145	20	digest	digest	NN
cord-048471-7jszm1nd	145	21	of	of	IN
cord-048471-7jszm1nd	145	22	both	both	DT
cord-048471-7jszm1nd	145	23	amplicon	amplicon	NN
cord-048471-7jszm1nd	145	24	and	and	CC
cord-048471-7jszm1nd	145	25	vector	vector	NN
cord-048471-7jszm1nd	145	26	with	with	IN
cord-048471-7jszm1nd	145	27	HindIII	HindIII	NNS
cord-048471-7jszm1nd	145	28	and	and	CC
cord-048471-7jszm1nd	145	29	ApaI	ApaI	NNP
cord-048471-7jszm1nd	145	30	restriction	restriction	NN
cord-048471-7jszm1nd	145	31	enzymes	enzyme	NNS
cord-048471-7jszm1nd	145	32	,	,	,
cord-048471-7jszm1nd	145	33	and	and	CC
cord-048471-7jszm1nd	145	34	the	the	DT
cord-048471-7jszm1nd	145	35	ligated	ligate	VBN
cord-048471-7jszm1nd	145	36	DNA	dna	NN
cord-048471-7jszm1nd	145	37	used	use	VBN
cord-048471-7jszm1nd	145	38	to	to	TO
cord-048471-7jszm1nd	145	39	transform	transform	VB
cord-048471-7jszm1nd	145	40	E.coli	E.coli	NNS
cord-048471-7jszm1nd	145	41	Top10	Top10	NNP
cord-048471-7jszm1nd	145	42	.	.	.
cord-048471-7jszm1nd	146	1	The	the	DT
cord-048471-7jszm1nd	146	2	JV	JV	NNP
cord-048471-7jszm1nd	146	3	3C	3c	NN
cord-048471-7jszm1nd	146	4	protease	protease	NN
cord-048471-7jszm1nd	146	5	mutant	mutant	NN
cord-048471-7jszm1nd	146	6	was	be	VBD
cord-048471-7jszm1nd	146	7	created	create	VBN
cord-048471-7jszm1nd	146	8	by	by	IN
cord-048471-7jszm1nd	146	9	point	point	NN
cord-048471-7jszm1nd	146	10	mutation	mutation	NN
cord-048471-7jszm1nd	146	11	of	of	IN
cord-048471-7jszm1nd	146	12	the	the	DT
cord-048471-7jszm1nd	146	13	critical	critical	JJ
cord-048471-7jszm1nd	146	14	TGT	TGT	NNP
cord-048471-7jszm1nd	146	15	encoded	encode	VBD
cord-048471-7jszm1nd	146	16	cysteine	cysteine	NN
cord-048471-7jszm1nd	146	17	residue	residue	NN
cord-048471-7jszm1nd	146	18	,	,	,
cord-048471-7jszm1nd	146	19	within	within	IN
cord-048471-7jszm1nd	146	20	the	the	DT
cord-048471-7jszm1nd	146	21	GDCG	GDCG	NNP
cord-048471-7jszm1nd	146	22	active	active	JJ
cord-048471-7jszm1nd	146	23	site	site	NN
cord-048471-7jszm1nd	146	24	motif	motif	NN
cord-048471-7jszm1nd	146	25	,	,	,
cord-048471-7jszm1nd	146	26	to	to	IN
cord-048471-7jszm1nd	146	27	a	a	DT
cord-048471-7jszm1nd	146	28	GGT	ggt	NN
cord-048471-7jszm1nd	146	29	encoded	encode	VBN
cord-048471-7jszm1nd	146	30	glycine	glycine	NN
cord-048471-7jszm1nd	146	31	residue	residue	NN
cord-048471-7jszm1nd	146	32	by	by	IN
cord-048471-7jszm1nd	146	33	mutagenic	mutagenic	JJ
cord-048471-7jszm1nd	146	34	overlap	overlap	NN
cord-048471-7jszm1nd	146	35	PCR	PCR	NNP
cord-048471-7jszm1nd	146	36	using	use	VBG
cord-048471-7jszm1nd	146	37	Bio	Bio	NNP
cord-048471-7jszm1nd	146	38	-	-	HYPH
cord-048471-7jszm1nd	146	39	X	X	NNP
cord-048471-7jszm1nd	146	40	-	-	HYPH
cord-048471-7jszm1nd	146	41	Act	act	NN
cord-048471-7jszm1nd	146	42	DNA	dna	NN
cord-048471-7jszm1nd	146	43	polymerase	polymerase	NN
cord-048471-7jszm1nd	146	44	.	.	.
cord-048471-7jszm1nd	147	1	Three	three	CD
cord-048471-7jszm1nd	147	2	rounds	round	NNS
cord-048471-7jszm1nd	147	3	of	of	IN
cord-048471-7jszm1nd	147	4	amplification	amplification	NN
cord-048471-7jszm1nd	147	5	using	use	VBG
cord-048471-7jszm1nd	147	6	the	the	DT
cord-048471-7jszm1nd	147	7	JV	JV	NNP
cord-048471-7jszm1nd	147	8	full	full	JJ
cord-048471-7jszm1nd	147	9	length	length	NN
cord-048471-7jszm1nd	147	10	cDNA	cdna	NN
cord-048471-7jszm1nd	147	11	clone	clone	NN
cord-048471-7jszm1nd	147	12	as	as	IN
cord-048471-7jszm1nd	147	13	template	template	NN
cord-048471-7jszm1nd	147	14	were	be	VBD
cord-048471-7jszm1nd	147	15	used	use	VBN
cord-048471-7jszm1nd	147	16	to	to	TO
cord-048471-7jszm1nd	147	17	generate	generate	VB
cord-048471-7jszm1nd	147	18	the	the	DT
cord-048471-7jszm1nd	147	19	final	final	JJ
cord-048471-7jszm1nd	147	20	mutant	mutant	JJ
cord-048471-7jszm1nd	147	21	protease	protease	NN
cord-048471-7jszm1nd	147	22	cassette	cassette	NN
cord-048471-7jszm1nd	147	23	.	.	.
cord-048471-7jszm1nd	148	1	Round	round	JJ
cord-048471-7jszm1nd	148	2	1	1	CD
cord-048471-7jszm1nd	148	3	used	use	VBD
cord-048471-7jszm1nd	148	4	the	the	DT
cord-048471-7jszm1nd	148	5	primers	primer	NNS
cord-048471-7jszm1nd	148	6	JV	JV	NNP
cord-048471-7jszm1nd	148	7	F1	F1	NNP
cord-048471-7jszm1nd	148	8	(	(	-LRB-
cord-048471-7jszm1nd	148	9	59-CGTCTCAGGGTTGATACT-39	59-CGTCTCAGGGTTGATACT-39	NNP
cord-048471-7jszm1nd	148	10	)	)	-RRB-
cord-048471-7jszm1nd	148	11	and	and	CC
cord-048471-7jszm1nd	148	12	JV	JV	NNP
cord-048471-7jszm1nd	148	13	Mut	Mut	NNP
cord-048471-7jszm1nd	148	14	1	1	CD
cord-048471-7jszm1nd	148	15	(	(	-LRB-
cord-048471-7jszm1nd	148	16	59-GCAACCACCGTCACCAG-39	59-GCAACCACCGTCACCAG-39	NNP
cord-048471-7jszm1nd	148	17	)	)	-RRB-
cord-048471-7jszm1nd	148	18	,	,	,
cord-048471-7jszm1nd	148	19	yielding	yield	VBG
cord-048471-7jszm1nd	148	20	a	a	DT
cord-048471-7jszm1nd	148	21	222	222	CD
cord-048471-7jszm1nd	148	22	bp	bp	NNP
cord-048471-7jszm1nd	148	23	amplicon	amplicon	NNP
cord-048471-7jszm1nd	148	24	(	(	-LRB-
cord-048471-7jszm1nd	148	25	point	point	NN
cord-048471-7jszm1nd	148	26	mutation	mutation	NN
cord-048471-7jszm1nd	148	27	nucleotide	nucleotide	NN
cord-048471-7jszm1nd	148	28	shown	show	VBN
cord-048471-7jszm1nd	148	29	in	in	IN
cord-048471-7jszm1nd	148	30	bold	bold	JJ
cord-048471-7jszm1nd	148	31	)	)	-RRB-
cord-048471-7jszm1nd	148	32	.	.	.
cord-048471-7jszm1nd	149	1	Round	round	JJ
cord-048471-7jszm1nd	149	2	2	2	CD
cord-048471-7jszm1nd	149	3	used	use	VBD
cord-048471-7jszm1nd	149	4	the	the	DT
cord-048471-7jszm1nd	149	5	primers	primer	NNS
cord-048471-7jszm1nd	149	6	JV	JV	NNP
cord-048471-7jszm1nd	149	7	Mut	Mut	NNP
cord-048471-7jszm1nd	149	8	2	2	CD
cord-048471-7jszm1nd	149	9	(	(	-LRB-
cord-048471-7jszm1nd	149	10	59-CTGGTGACGGT	59-ctggtgacggt	CD
cord-048471-7jszm1nd	149	11	-	-	HYPH
cord-048471-7jszm1nd	149	12	GGTTGC-39	ggttgc-39	NN
cord-048471-7jszm1nd	149	13	)	)	-RRB-
cord-048471-7jszm1nd	149	14	and	and	CC
cord-048471-7jszm1nd	149	15	JV	JV	NNP
cord-048471-7jszm1nd	149	16	R2	R2	NNP
cord-048471-7jszm1nd	149	17	(	(	-LRB-
cord-048471-7jszm1nd	149	18	59-TTCCTGGGAGGAACAAGTT-39	59-TTCCTGGGAGGAACAAGTT-39	NNP
cord-048471-7jszm1nd	149	19	)	)	-RRB-
cord-048471-7jszm1nd	149	20	,	,	,
cord-048471-7jszm1nd	149	21	yielding	yield	VBG
cord-048471-7jszm1nd	149	22	a	a	DT
cord-048471-7jszm1nd	149	23	651	651	CD
cord-048471-7jszm1nd	149	24	bp	bp	NNP
cord-048471-7jszm1nd	149	25	amplicon	amplicon	NNP
cord-048471-7jszm1nd	149	26	.	.	.
cord-048471-7jszm1nd	150	1	Amplicons	Amplicons	NNP
cord-048471-7jszm1nd	150	2	generated	generate	VBN
cord-048471-7jszm1nd	150	3	in	in	IN
cord-048471-7jszm1nd	150	4	rounds	round	NNS
cord-048471-7jszm1nd	150	5	1	1	CD
cord-048471-7jszm1nd	150	6	and	and	CC
cord-048471-7jszm1nd	150	7	2	2	CD
cord-048471-7jszm1nd	150	8	were	be	VBD
cord-048471-7jszm1nd	150	9	pooled	pool	VBN
cord-048471-7jszm1nd	150	10	to	to	TO
cord-048471-7jszm1nd	150	11	serve	serve	VB
cord-048471-7jszm1nd	150	12	as	as	IN
cord-048471-7jszm1nd	150	13	template	template	NN
cord-048471-7jszm1nd	150	14	for	for	IN
cord-048471-7jszm1nd	150	15	round	round	JJ
cord-048471-7jszm1nd	150	16	3	3	CD
cord-048471-7jszm1nd	150	17	using	use	VBG
cord-048471-7jszm1nd	150	18	the	the	DT
cord-048471-7jszm1nd	150	19	primers	primer	NNS
cord-048471-7jszm1nd	150	20	JV	JV	NNP
cord-048471-7jszm1nd	150	21	NF	NF	NNP
cord-048471-7jszm1nd	150	22	(	(	-LRB-
cord-048471-7jszm1nd	150	23	59-ATGTCAACCACCACCAGC	59-atgtcaaccaccaccagc	CD
cord-048471-7jszm1nd	150	24	-39	-39	NN
cord-048471-7jszm1nd	150	25	)	)	-RRB-
cord-048471-7jszm1nd	150	26	and	and	CC
cord-048471-7jszm1nd	150	27	JV	JV	NNP
cord-048471-7jszm1nd	150	28	NR	NR	NNP
cord-048471-7jszm1nd	150	29	(	(	-LRB-
cord-048471-7jszm1nd	150	30	59-AAGGGCTCCGGTGAAGG-39	59-AAGGGCTCCGGTGAAGG-39	NNP
cord-048471-7jszm1nd	150	31	)	)	-RRB-
cord-048471-7jszm1nd	150	32	.	.	.
cord-048471-7jszm1nd	151	1	This	this	DT
cord-048471-7jszm1nd	151	2	cassette	cassette	NN
cord-048471-7jszm1nd	151	3	contained	contain	VBD
cord-048471-7jszm1nd	151	4	two	two	CD
cord-048471-7jszm1nd	151	5	BclI	BclI	NNP
cord-048471-7jszm1nd	151	6	restriction	restriction	NN
cord-048471-7jszm1nd	151	7	sites	site	NNS
cord-048471-7jszm1nd	151	8	flanking	flank	VBG
cord-048471-7jszm1nd	151	9	the	the	DT
cord-048471-7jszm1nd	151	10	3Cprotease	3cprotease	CD
cord-048471-7jszm1nd	151	11	active	active	JJ
cord-048471-7jszm1nd	151	12	site	site	NN
cord-048471-7jszm1nd	151	13	,	,	,
cord-048471-7jszm1nd	151	14	as	as	IN
cord-048471-7jszm1nd	151	15	also	also	RB
cord-048471-7jszm1nd	151	16	found	find	VBN
cord-048471-7jszm1nd	151	17	in	in	IN
cord-048471-7jszm1nd	151	18	the	the	DT
cord-048471-7jszm1nd	151	19	wild	wild	JJ
cord-048471-7jszm1nd	151	20	-	-	HYPH
cord-048471-7jszm1nd	151	21	type	type	NN
cord-048471-7jszm1nd	151	22	full	full	JJ
cord-048471-7jszm1nd	151	23	length	length	NN
cord-048471-7jszm1nd	151	24	clone	clone	NN
cord-048471-7jszm1nd	151	25	.	.	.
cord-048471-7jszm1nd	152	1	Restriction	restriction	NN
cord-048471-7jszm1nd	152	2	digest	digest	NN
cord-048471-7jszm1nd	152	3	using	use	VBG
cord-048471-7jszm1nd	152	4	BclI	BclI	NNP
cord-048471-7jszm1nd	152	5	was	be	VBD
cord-048471-7jszm1nd	152	6	used	use	VBN
cord-048471-7jszm1nd	152	7	to	to	TO
cord-048471-7jszm1nd	152	8	remove	remove	VB
cord-048471-7jszm1nd	152	9	the	the	DT
cord-048471-7jszm1nd	152	10	appropriate	appropriate	JJ
cord-048471-7jszm1nd	152	11	wild	wild	JJ
cord-048471-7jszm1nd	152	12	-	-	HYPH
cord-048471-7jszm1nd	152	13	type	type	NN
cord-048471-7jszm1nd	152	14	cassette	cassette	NN
cord-048471-7jszm1nd	152	15	from	from	IN
cord-048471-7jszm1nd	152	16	the	the	DT
cord-048471-7jszm1nd	152	17	JV	JV	NNP
cord-048471-7jszm1nd	152	18	full	full	JJ
cord-048471-7jszm1nd	152	19	length	length	NN
cord-048471-7jszm1nd	152	20	clone	clone	NN
cord-048471-7jszm1nd	152	21	.	.	.
cord-048471-7jszm1nd	153	1	The	the	DT
cord-048471-7jszm1nd	153	2	mutant	mutant	JJ
cord-048471-7jszm1nd	153	3	cassette	cassette	NN
cord-048471-7jszm1nd	153	4	was	be	VBD
cord-048471-7jszm1nd	153	5	also	also	RB
cord-048471-7jszm1nd	153	6	digested	digest	VBN
cord-048471-7jszm1nd	153	7	with	with	IN
cord-048471-7jszm1nd	153	8	BclI	BclI	NNP
cord-048471-7jszm1nd	153	9	prior	prior	RB
cord-048471-7jszm1nd	153	10	to	to	IN
cord-048471-7jszm1nd	153	11	ligation	ligation	NN
cord-048471-7jszm1nd	153	12	to	to	IN
cord-048471-7jszm1nd	153	13	the	the	DT
cord-048471-7jszm1nd	153	14	BclI	BclI	NNP
cord-048471-7jszm1nd	153	15	-	-	HYPH
cord-048471-7jszm1nd	153	16	digested	digest	VBN
cord-048471-7jszm1nd	153	17	JV	JV	NNP
cord-048471-7jszm1nd	153	18	full	full	JJ
cord-048471-7jszm1nd	153	19	length	length	NN
cord-048471-7jszm1nd	153	20	clone	clone	NN
cord-048471-7jszm1nd	153	21	.	.	.
cord-048471-7jszm1nd	154	1	The	the	DT
cord-048471-7jszm1nd	154	2	ligated	ligate	VBN
cord-048471-7jszm1nd	154	3	DNA	dna	NN
cord-048471-7jszm1nd	154	4	was	be	VBD
cord-048471-7jszm1nd	154	5	used	use	VBN
cord-048471-7jszm1nd	154	6	to	to	TO
cord-048471-7jszm1nd	154	7	transform	transform	VB
cord-048471-7jszm1nd	154	8	E.coli	E.coli	NNS
cord-048471-7jszm1nd	154	9	Top10	Top10	NNP
cord-048471-7jszm1nd	154	10	,	,	,
cord-048471-7jszm1nd	154	11	and	and	CC
cord-048471-7jszm1nd	154	12	was	be	VBD
cord-048471-7jszm1nd	154	13	designated	designate	VBN
cord-048471-7jszm1nd	154	14	JV	JV	NNP
cord-048471-7jszm1nd	154	15	3C	3c	NN
cord-048471-7jszm1nd	154	16	mut	mut	NNP
cord-048471-7jszm1nd	154	17	.	.	.
cord-048471-7jszm1nd	155	1	Construction	construction	NN
cord-048471-7jszm1nd	155	2	of	of	IN
cord-048471-7jszm1nd	155	3	JV	JV	NNP
cord-048471-7jszm1nd	155	4	3C	3C	NNP
cord-048471-7jszm1nd	155	5	mut	mut	NNP
cord-048471-7jszm1nd	155	6	/polySTOP	/polySTOP	NNP
cord-048471-7jszm1nd	155	7	was	be	VBD
cord-048471-7jszm1nd	155	8	as	as	IN
cord-048471-7jszm1nd	155	9	follows	follow	VBZ
cord-048471-7jszm1nd	155	10	:	:	:
cord-048471-7jszm1nd	155	11	complementary	complementary	JJ
cord-048471-7jszm1nd	155	12	oligonucleotides	oligonucleotide	NNS
cord-048471-7jszm1nd	155	13	with	with	IN
cord-048471-7jszm1nd	155	14	three	three	CD
cord-048471-7jszm1nd	155	15	translation	translation	NN
cord-048471-7jszm1nd	155	16	termination	termination	NN
cord-048471-7jszm1nd	155	17	codons	codon	NNS
cord-048471-7jszm1nd	155	18	(	(	-LRB-
cord-048471-7jszm1nd	155	19	underlined	underline	VBN
cord-048471-7jszm1nd	155	20	)	)	-RRB-
cord-048471-7jszm1nd	155	21	in	in	IN
cord-048471-7jszm1nd	155	22	each	each	DT
cord-048471-7jszm1nd	155	23	reading	reading	NN
cord-048471-7jszm1nd	155	24	frame	frame	NN
cord-048471-7jszm1nd	155	25	in	in	IN
cord-048471-7jszm1nd	155	26	sense	sense	NN
cord-048471-7jszm1nd	155	27	and	and	CC
cord-048471-7jszm1nd	155	28	anti	anti	JJ
cord-048471-7jszm1nd	155	29	-	-	JJ
cord-048471-7jszm1nd	155	30	sense	sense	JJ
cord-048471-7jszm1nd	155	31	orientations	orientation	NNS
cord-048471-7jszm1nd	155	32	were	be	VBD
cord-048471-7jszm1nd	155	33	desgined	desgine	VBN
cord-048471-7jszm1nd	155	34	in	in	IN
cord-048471-7jszm1nd	155	35	such	such	PDT
cord-048471-7jszm1nd	155	36	a	a	DT
cord-048471-7jszm1nd	155	37	way	way	NN
cord-048471-7jszm1nd	155	38	that	that	IN
cord-048471-7jszm1nd	155	39	upon	upon	IN
cord-048471-7jszm1nd	155	40	annealing	anneal	VBG
cord-048471-7jszm1nd	155	41	the	the	DT
cord-048471-7jszm1nd	155	42	duplex	duplex	NN
cord-048471-7jszm1nd	155	43	would	would	MD
cord-048471-7jszm1nd	155	44	contain	contain	VB
cord-048471-7jszm1nd	155	45	blunt	blunt	JJ
cord-048471-7jszm1nd	155	46	termini	terminus	NNS
cord-048471-7jszm1nd	155	47	.	.	.
cord-048471-7jszm1nd	156	1	The	the	DT
cord-048471-7jszm1nd	156	2	oligonucleotides	oligonucleotide	NNS
cord-048471-7jszm1nd	156	3	were	be	VBD
cord-048471-7jszm1nd	156	4	termed	term	VBN
cord-048471-7jszm1nd	156	5	pSTOP	pSTOP	NNP
cord-048471-7jszm1nd	156	6	Top	Top	NNP
cord-048471-7jszm1nd	156	7	(	(	-LRB-
cord-048471-7jszm1nd	156	8	59-CTAGGTAAGTAAACGCGTCTACT	59-ctaggtaagtaaacgcgtctact	CD
cord-048471-7jszm1nd	156	9	-	-	HYPH
cord-048471-7jszm1nd	156	10	CACTCAC-39	cactcac-39	CD
cord-048471-7jszm1nd	156	11	)	)	-RRB-
cord-048471-7jszm1nd	156	12	and	and	CC
cord-048471-7jszm1nd	156	13	pSTOP	pSTOP	NNP
cord-048471-7jszm1nd	156	14	Comp	Comp	NNP
cord-048471-7jszm1nd	156	15	(	(	-LRB-
cord-048471-7jszm1nd	156	16	59-GTGAGTGAGTA	59-gtgagtgagta	CD
cord-048471-7jszm1nd	156	17	-	-	HYPH
cord-048471-7jszm1nd	156	18	GACGCGTTTACTTCAATAG-39	GACGCGTTTACTTCAATAG-39	NNP
cord-048471-7jszm1nd	156	19	)	)	-RRB-
cord-048471-7jszm1nd	156	20	.	.	.
cord-048471-7jszm1nd	157	1	Each	each	DT
cord-048471-7jszm1nd	157	2	oligo	oligo	NN
cord-048471-7jszm1nd	157	3	(	(	-LRB-
cord-048471-7jszm1nd	157	4	1	1	CD
cord-048471-7jszm1nd	157	5	mg	mg	CD
cord-048471-7jszm1nd	157	6	)	)	-RRB-
cord-048471-7jszm1nd	157	7	was	be	VBD
cord-048471-7jszm1nd	157	8	incubated	incubate	VBN
cord-048471-7jszm1nd	157	9	with	with	IN
cord-048471-7jszm1nd	157	10	T4	T4	NNP
cord-048471-7jszm1nd	157	11	polynucleotide	polynucleotide	NNP
cord-048471-7jszm1nd	157	12	kinase	kinase	NN
cord-048471-7jszm1nd	157	13	and	and	CC
cord-048471-7jszm1nd	157	14	ATP	ATP	NNP
cord-048471-7jszm1nd	157	15	to	to	TO
cord-048471-7jszm1nd	157	16	phosphorylate	phosphorylate	VB
cord-048471-7jszm1nd	157	17	the	the	DT
cord-048471-7jszm1nd	157	18	59	59	CD
cord-048471-7jszm1nd	157	19	termini	terminus	NNS
cord-048471-7jszm1nd	157	20	,	,	,
cord-048471-7jszm1nd	157	21	pooled	pool	VBN
cord-048471-7jszm1nd	157	22	and	and	CC
cord-048471-7jszm1nd	157	23	heated	heat	VBN
cord-048471-7jszm1nd	157	24	to	to	IN
cord-048471-7jszm1nd	157	25	75uC	75uc	CD
cord-048471-7jszm1nd	157	26	for	for	IN
cord-048471-7jszm1nd	157	27	15	15	CD
cord-048471-7jszm1nd	157	28	min	min	NN
cord-048471-7jszm1nd	157	29	,	,	,
cord-048471-7jszm1nd	157	30	and	and	CC
cord-048471-7jszm1nd	157	31	left	leave	VBD
cord-048471-7jszm1nd	157	32	to	to	TO
cord-048471-7jszm1nd	157	33	cool	cool	VB
cord-048471-7jszm1nd	157	34	to	to	IN
cord-048471-7jszm1nd	157	35	room	room	NN
cord-048471-7jszm1nd	157	36	temperature	temperature	NN
cord-048471-7jszm1nd	157	37	to	to	TO
cord-048471-7jszm1nd	157	38	anneal	anneal	VB
cord-048471-7jszm1nd	157	39	the	the	DT
cord-048471-7jszm1nd	157	40	oligos	oligo	NNS
cord-048471-7jszm1nd	157	41	.	.	.
cord-048471-7jszm1nd	158	1	Following	follow	VBG
cord-048471-7jszm1nd	158	2	purification	purification	NN
cord-048471-7jszm1nd	158	3	the	the	DT
cord-048471-7jszm1nd	158	4	polySTOP	polySTOP	NNP
cord-048471-7jszm1nd	158	5	duplex	duplex	NNP
cord-048471-7jszm1nd	158	6	was	be	VBD
cord-048471-7jszm1nd	158	7	ligated	ligate	VBN
cord-048471-7jszm1nd	158	8	to	to	IN
cord-048471-7jszm1nd	159	1	Eco47III	Eco47III	NNP
cord-048471-7jszm1nd	159	2	digested	digest	VBD
cord-048471-7jszm1nd	159	3	JV	JV	NNP
cord-048471-7jszm1nd	159	4	3C	3C	NNP
cord-048471-7jszm1nd	159	5	mut	mut	NNP
cord-048471-7jszm1nd	159	6	,	,	,
cord-048471-7jszm1nd	159	7	and	and	CC
cord-048471-7jszm1nd	159	8	ligated	ligate	VBN
cord-048471-7jszm1nd	159	9	DNA	DNA	NNP
cord-048471-7jszm1nd	159	10	was	be	VBD
cord-048471-7jszm1nd	159	11	used	use	VBN
cord-048471-7jszm1nd	159	12	to	to	TO
cord-048471-7jszm1nd	159	13	transform	transform	VB
cord-048471-7jszm1nd	159	14	E.coli	E.coli	NNS
cord-048471-7jszm1nd	159	15	Top10	Top10	NNP
cord-048471-7jszm1nd	159	16	.	.	.
cord-048471-7jszm1nd	160	1	The	the	DT
cord-048471-7jszm1nd	160	2	duplex	duplex	NN
cord-048471-7jszm1nd	160	3	contained	contain	VBD
cord-048471-7jszm1nd	160	4	the	the	DT
cord-048471-7jszm1nd	160	5	unique	unique	JJ
cord-048471-7jszm1nd	160	6	restriction	restriction	NN
cord-048471-7jszm1nd	160	7	site	site	NN
cord-048471-7jszm1nd	160	8	MluI	MluI	NNP
cord-048471-7jszm1nd	160	9	(	(	-LRB-
cord-048471-7jszm1nd	160	10	shown	show	VBN
cord-048471-7jszm1nd	160	11	in	in	IN
cord-048471-7jszm1nd	160	12	bold	bold	JJ
cord-048471-7jszm1nd	160	13	)	)	-RRB-
cord-048471-7jszm1nd	160	14	to	to	TO
cord-048471-7jszm1nd	160	15	assist	assist	VB
cord-048471-7jszm1nd	160	16	screening	screening	NN
cord-048471-7jszm1nd	160	17	of	of	IN
cord-048471-7jszm1nd	160	18	recombinant	recombinant	JJ
cord-048471-7jszm1nd	160	19	clones	clone	NNS
cord-048471-7jszm1nd	160	20	.	.	.
cord-048471-7jszm1nd	161	1	The	the	DT
cord-048471-7jszm1nd	161	2	V5	v5	NN
cord-048471-7jszm1nd	161	3	epitope	epitope	NN
cord-048471-7jszm1nd	161	4	(	(	-LRB-
cord-048471-7jszm1nd	161	5	N	N	NNP
cord-048471-7jszm1nd	161	6	-	-	HYPH
cord-048471-7jszm1nd	161	7	Gly	Gly	NNP
cord-048471-7jszm1nd	161	8	-	-	HYPH
cord-048471-7jszm1nd	161	9	Lys	Lys	NNP
cord-048471-7jszm1nd	161	10	-	-	HYPH
cord-048471-7jszm1nd	161	11	Pro	Pro	NNP
cord-048471-7jszm1nd	161	12	-	-	HYPH
cord-048471-7jszm1nd	161	13	Ile	Ile	NNP
cord-048471-7jszm1nd	161	14	-	-	HYPH
cord-048471-7jszm1nd	161	15	Pro	Pro	NNP
cord-048471-7jszm1nd	161	16	-	-	NNP
cord-048471-7jszm1nd	161	17	Asn	Asn	NNP
cord-048471-7jszm1nd	161	18	-	-	HYPH
cord-048471-7jszm1nd	161	19	Pro	Pro	NNP
cord-048471-7jszm1nd	161	20	-	-	NNP
cord-048471-7jszm1nd	161	21	Leu	Leu	NNP
cord-048471-7jszm1nd	161	22	-	-	HYPH
cord-048471-7jszm1nd	161	23	Leu	Leu	NNP
cord-048471-7jszm1nd	161	24	-	-	HYPH
cord-048471-7jszm1nd	161	25	Gly	Gly	NNP
cord-048471-7jszm1nd	161	26	-	-	HYPH
cord-048471-7jszm1nd	161	27	Leu	Leu	NNP
cord-048471-7jszm1nd	161	28	-	-	HYPH
cord-048471-7jszm1nd	161	29	Asp	Asp	NNP
cord-048471-7jszm1nd	161	30	-	-	HYPH
cord-048471-7jszm1nd	161	31	Ser	Ser	NNP
cord-048471-7jszm1nd	161	32	-	-	HYPH
cord-048471-7jszm1nd	161	33	Thr	Thr	NNP
cord-048471-7jszm1nd	161	34	-	-	HYPH
cord-048471-7jszm1nd	161	35	C	C	NNP
cord-048471-7jszm1nd	161	36	)	)	-RRB-
cord-048471-7jszm1nd	161	37	is	be	VBZ
cord-048471-7jszm1nd	161	38	recognized	recognize	VBN
cord-048471-7jszm1nd	161	39	by	by	IN
cord-048471-7jszm1nd	161	40	the	the	DT
cord-048471-7jszm1nd	161	41	anti	anti	JJ
cord-048471-7jszm1nd	161	42	-	-	JJ
cord-048471-7jszm1nd	161	43	V5	v5	JJ
cord-048471-7jszm1nd	161	44	monoclonal	monoclonal	JJ
cord-048471-7jszm1nd	161	45	antibody	antibody	NN
cord-048471-7jszm1nd	161	46	(	(	-LRB-
cord-048471-7jszm1nd	161	47	Invitrogen	Invitrogen	NNP
cord-048471-7jszm1nd	161	48	)	)	-RRB-
cord-048471-7jszm1nd	161	49	.	.	.
cord-048471-7jszm1nd	162	1	Complementary	complementary	JJ
cord-048471-7jszm1nd	162	2	oligonucleotides	oligonucleotide	NNS
cord-048471-7jszm1nd	162	3	encoding	encode	VBG
cord-048471-7jszm1nd	162	4	the	the	DT
cord-048471-7jszm1nd	162	5	V5	V5	NNP
cord-048471-7jszm1nd	162	6	epitope	epitope	NN
cord-048471-7jszm1nd	162	7	were	be	VBD
cord-048471-7jszm1nd	162	8	designed	design	VBN
cord-048471-7jszm1nd	162	9	in	in	IN
cord-048471-7jszm1nd	162	10	such	such	PDT
cord-048471-7jszm1nd	162	11	a	a	DT
cord-048471-7jszm1nd	162	12	way	way	NN
cord-048471-7jszm1nd	162	13	as	as	IN
cord-048471-7jszm1nd	162	14	to	to	TO
cord-048471-7jszm1nd	162	15	generate	generate	VB
cord-048471-7jszm1nd	162	16	SacII	SacII	NNP
cord-048471-7jszm1nd	162	17	compatible	compatible	JJ
cord-048471-7jszm1nd	162	18	termini	termini	NN
cord-048471-7jszm1nd	162	19	following	follow	VBG
cord-048471-7jszm1nd	162	20	annealing	anneal	VBG
cord-048471-7jszm1nd	162	21	(	(	-LRB-
cord-048471-7jszm1nd	162	22	bold	bold	JJ
cord-048471-7jszm1nd	162	23	)	)	-RRB-
cord-048471-7jszm1nd	162	24	,	,	,
cord-048471-7jszm1nd	162	25	and	and	CC
cord-048471-7jszm1nd	162	26	to	to	TO
cord-048471-7jszm1nd	162	27	preserve	preserve	VB
cord-048471-7jszm1nd	162	28	the	the	DT
cord-048471-7jszm1nd	162	29	reading	reading	NN
cord-048471-7jszm1nd	162	30	frame	frame	NN
cord-048471-7jszm1nd	162	31	when	when	WRB
cord-048471-7jszm1nd	162	32	inserted	insert	VBN
cord-048471-7jszm1nd	162	33	into	into	IN
cord-048471-7jszm1nd	162	34	the	the	DT
cord-048471-7jszm1nd	162	35	SacII	SacII	NNP
cord-048471-7jszm1nd	162	36	restriction	restriction	NN
cord-048471-7jszm1nd	162	37	site	site	NN
cord-048471-7jszm1nd	162	38	at	at	IN
cord-048471-7jszm1nd	162	39	nucleotide	nucleotide	JJ
cord-048471-7jszm1nd	162	40	123	123	CD
cord-048471-7jszm1nd	162	41	within	within	IN
cord-048471-7jszm1nd	162	42	the	the	DT
cord-048471-7jszm1nd	162	43	59	59	CD
cord-048471-7jszm1nd	162	44	GS	g	NNS
cord-048471-7jszm1nd	162	45	of	of	IN
cord-048471-7jszm1nd	162	46	the	the	DT
cord-048471-7jszm1nd	162	47	JV	JV	NNP
cord-048471-7jszm1nd	162	48	genome	genome	NN
cord-048471-7jszm1nd	162	49	(	(	-LRB-
cord-048471-7jszm1nd	162	50	underlined	underline	VBN
cord-048471-7jszm1nd	162	51	)	)	-RRB-
cord-048471-7jszm1nd	162	52	.	.	.
cord-048471-7jszm1nd	163	1	The	the	DT
cord-048471-7jszm1nd	163	2	oligos	oligo	NNS
cord-048471-7jszm1nd	163	3	were	be	VBD
cord-048471-7jszm1nd	163	4	termed	term	VBN
cord-048471-7jszm1nd	163	5	V5	V5	NNP
cord-048471-7jszm1nd	163	6	Top	Top	NNP
cord-048471-7jszm1nd	163	7	(	(	-LRB-
cord-048471-7jszm1nd	163	8	59-GGTAAGCCTATCCC	59-ggtaagcctatccc	CD
cord-048471-7jszm1nd	163	9	-	-	HYPH
cord-048471-7jszm1nd	163	10	TAACCCTCTCCTCGGTCTCGATTCTACGAGC-39	taaccctctcctcggtctcgattctacgagc-39	CD
cord-048471-7jszm1nd	163	11	)	)	-RRB-
cord-048471-7jszm1nd	163	12	and	and	CC
cord-048471-7jszm1nd	163	13	V5	V5	NNP
cord-048471-7jszm1nd	163	14	Comp	Comp	NNP
cord-048471-7jszm1nd	163	15	(	(	-LRB-
cord-048471-7jszm1nd	163	16	59-TCGTAGAATCGAGACCGAGGAGAGGGT	59-tcgtagaatcgagaccgaggagagggt	CD
cord-048471-7jszm1nd	163	17	-	-	HYPH
cord-048471-7jszm1nd	163	18	TAGGGATAGGCTTACCGC-39	tagggataggcttaccgc-39	CD
cord-048471-7jszm1nd	163	19	)	)	-RRB-
cord-048471-7jszm1nd	163	20	.	.	.
cord-048471-7jszm1nd	164	1	The	the	DT
cord-048471-7jszm1nd	164	2	oligos	oligo	NNS
cord-048471-7jszm1nd	164	3	were	be	VBD
cord-048471-7jszm1nd	164	4	phosphorylated	phosphorylate	VBN
cord-048471-7jszm1nd	164	5	and	and	CC
cord-048471-7jszm1nd	164	6	annealed	anneal	VBN
cord-048471-7jszm1nd	164	7	as	as	IN
cord-048471-7jszm1nd	164	8	described	describe	VBN
cord-048471-7jszm1nd	164	9	previously	previously	RB
cord-048471-7jszm1nd	164	10	and	and	CC
cord-048471-7jszm1nd	164	11	the	the	DT
cord-048471-7jszm1nd	164	12	duplex	duplex	NN
cord-048471-7jszm1nd	164	13	ligated	ligate	VBN
cord-048471-7jszm1nd	164	14	to	to	IN
cord-048471-7jszm1nd	164	15	the	the	DT
cord-048471-7jszm1nd	164	16	SacII	SacII	NNP
cord-048471-7jszm1nd	164	17	digested	digest	VBD
cord-048471-7jszm1nd	164	18	JV	JV	NNP
cord-048471-7jszm1nd	164	19	full	full	JJ
cord-048471-7jszm1nd	164	20	length	length	NN
cord-048471-7jszm1nd	164	21	clone	clone	NN
cord-048471-7jszm1nd	164	22	.	.	.
cord-048471-7jszm1nd	165	1	Ligated	Ligated	NNP
cord-048471-7jszm1nd	165	2	DNA	DNA	NNP
cord-048471-7jszm1nd	165	3	was	be	VBD
cord-048471-7jszm1nd	165	4	used	use	VBN
cord-048471-7jszm1nd	165	5	to	to	TO
cord-048471-7jszm1nd	165	6	transform	transform	VB
cord-048471-7jszm1nd	165	7	E.coli	E.coli	NNS
cord-048471-7jszm1nd	165	8	Top10	Top10	NNP
cord-048471-7jszm1nd	165	9	.	.	.
cord-048471-7jszm1nd	166	1	In	in	IN
cord-048471-7jszm1nd	166	2	vitro	vitro	FW
cord-048471-7jszm1nd	166	3	coupled	couple	VBN
cord-048471-7jszm1nd	166	4	transcription	transcription	NN
cord-048471-7jszm1nd	166	5	and	and	CC
cord-048471-7jszm1nd	166	6	translation	translation	NN
cord-048471-7jszm1nd	166	7	was	be	VBD
cord-048471-7jszm1nd	166	8	performed	perform	VBN
cord-048471-7jszm1nd	166	9	using	use	VBG
cord-048471-7jszm1nd	166	10	the	the	DT
cord-048471-7jszm1nd	166	11	TNTH	TNTH	NNP
cord-048471-7jszm1nd	166	12	Coupled	Coupled	NNP
cord-048471-7jszm1nd	166	13	Reticulocyte	Reticulocyte	NNP
cord-048471-7jszm1nd	166	14	Lysate	Lysate	NNP
cord-048471-7jszm1nd	166	15	System	System	NNP
cord-048471-7jszm1nd	166	16	(	(	-LRB-
cord-048471-7jszm1nd	166	17	Promega	Promega	NNP
cord-048471-7jszm1nd	166	18	)	)	-RRB-
cord-048471-7jszm1nd	166	19	as	as	IN
cord-048471-7jszm1nd	166	20	per	per	IN
cord-048471-7jszm1nd	166	21	the	the	DT
cord-048471-7jszm1nd	166	22	manufacturer	manufacturer	NN
cord-048471-7jszm1nd	166	23	's	's	POS
cord-048471-7jszm1nd	166	24	instructions	instruction	NNS
cord-048471-7jszm1nd	166	25	.	.	.
cord-048471-7jszm1nd	167	1	Reactions	reaction	NNS
cord-048471-7jszm1nd	167	2	were	be	VBD
cord-048471-7jszm1nd	167	3	incubated	incubate	VBN
cord-048471-7jszm1nd	167	4	at	at	IN
cord-048471-7jszm1nd	167	5	30uC	30uc	CD
cord-048471-7jszm1nd	167	6	for	for	IN
cord-048471-7jszm1nd	167	7	1	1	CD
cord-048471-7jszm1nd	167	8	-	-	SYM
cord-048471-7jszm1nd	167	9	2	2	CD
cord-048471-7jszm1nd	167	10	hr	hr	NN
cord-048471-7jszm1nd	167	11	.	.	.
cord-048471-7jszm1nd	168	1	For	for	IN
cord-048471-7jszm1nd	168	2	non	non	JJ
cord-048471-7jszm1nd	168	3	-	-	JJ
cord-048471-7jszm1nd	168	4	radiolabelled	radiolabelled	JJ
cord-048471-7jszm1nd	168	5	reactions	reaction	NNS
cord-048471-7jszm1nd	168	6	the	the	DT
cord-048471-7jszm1nd	168	7	35	35	CD
cord-048471-7jszm1nd	168	8	S	S	NNP
cord-048471-7jszm1nd	168	9	-	-	HYPH
cord-048471-7jszm1nd	168	10	Methionine	Methionine	NNP
cord-048471-7jszm1nd	168	11	was	be	VBD
cord-048471-7jszm1nd	168	12	replaced	replace	VBN
cord-048471-7jszm1nd	168	13	with	with	IN
cord-048471-7jszm1nd	168	14	1	1	CD
cord-048471-7jszm1nd	168	15	mM	mm	CD
cord-048471-7jszm1nd	168	16	unlabelled	unlabelled	JJ
cord-048471-7jszm1nd	168	17	Methionine	methionine	NN
cord-048471-7jszm1nd	168	18	(	(	-LRB-
cord-048471-7jszm1nd	168	19	2	2	CD
cord-048471-7jszm1nd	168	20	ml	ml	NN
cord-048471-7jszm1nd	168	21	)	)	-RRB-
cord-048471-7jszm1nd	168	22	.	.	.
cord-048471-7jszm1nd	169	1	Reaction	reaction	NN
cord-048471-7jszm1nd	169	2	products	product	NNS
cord-048471-7jszm1nd	169	3	(	(	-LRB-
cord-048471-7jszm1nd	169	4	1	1	CD
cord-048471-7jszm1nd	169	5	-	-	SYM
cord-048471-7jszm1nd	169	6	2	2	CD
cord-048471-7jszm1nd	169	7	ml	ml	NNS
cord-048471-7jszm1nd	169	8	)	)	-RRB-
cord-048471-7jszm1nd	169	9	were	be	VBD
cord-048471-7jszm1nd	169	10	analysed	analyse	VBN
cord-048471-7jszm1nd	169	11	by	by	IN
cord-048471-7jszm1nd	169	12	SDS	SDS	NNP
cord-048471-7jszm1nd	169	13	-	-	HYPH
cord-048471-7jszm1nd	169	14	PAGE	PAGE	NNP
cord-048471-7jszm1nd	169	15	.	.	.
cord-048471-7jszm1nd	170	1	Gels	gel	NNS
cord-048471-7jszm1nd	170	2	were	be	VBD
cord-048471-7jszm1nd	170	3	stained	stain	VBN
cord-048471-7jszm1nd	170	4	and	and	CC
cord-048471-7jszm1nd	170	5	prepared	prepare	VBN
cord-048471-7jszm1nd	170	6	for	for	IN
cord-048471-7jszm1nd	170	7	autoradiography	autoradiography	NNP
cord-048471-7jszm1nd	170	8	by	by	IN
cord-048471-7jszm1nd	170	9	incubating	incubate	VBG
cord-048471-7jszm1nd	170	10	for	for	IN
cord-048471-7jszm1nd	170	11	30	30	CD
cord-048471-7jszm1nd	170	12	min	min	NN
cord-048471-7jszm1nd	170	13	in	in	IN
cord-048471-7jszm1nd	170	14	a	a	DT
cord-048471-7jszm1nd	170	15	solution	solution	NN
cord-048471-7jszm1nd	170	16	containing	contain	VBG
cord-048471-7jszm1nd	170	17	32	32	CD
cord-048471-7jszm1nd	170	18	g	g	NN
cord-048471-7jszm1nd	170	19	sodium	sodium	NN
cord-048471-7jszm1nd	170	20	salicylate	salicylate	NN
cord-048471-7jszm1nd	170	21	,	,	,
cord-048471-7jszm1nd	170	22	100	100	CD
cord-048471-7jszm1nd	170	23	ml	ml	NN
cord-048471-7jszm1nd	170	24	methanol	methanol	NN
cord-048471-7jszm1nd	170	25	and	and	CC
cord-048471-7jszm1nd	170	26	100	100	CD
cord-048471-7jszm1nd	170	27	ml	ml	NN
cord-048471-7jszm1nd	171	1	dH	dH	NNP
cord-048471-7jszm1nd	171	2	2	2	CD
cord-048471-7jszm1nd	171	3	O.	O.	NNP
cord-048471-7jszm1nd	171	4	Gels	Gels	NNPS
cord-048471-7jszm1nd	171	5	were	be	VBD
cord-048471-7jszm1nd	171	6	dried	dry	VBN
cord-048471-7jszm1nd	171	7	under	under	IN
cord-048471-7jszm1nd	171	8	vacuum	vacuum	NN
cord-048471-7jszm1nd	171	9	and	and	CC
cord-048471-7jszm1nd	171	10	the	the	DT
cord-048471-7jszm1nd	171	11	reaction	reaction	NN
cord-048471-7jszm1nd	171	12	products	product	NNS
cord-048471-7jszm1nd	171	13	were	be	VBD
cord-048471-7jszm1nd	171	14	detected	detect	VBN
cord-048471-7jszm1nd	171	15	by	by	IN
cord-048471-7jszm1nd	171	16	exposure	exposure	NN
cord-048471-7jszm1nd	171	17	to	to	IN
cord-048471-7jszm1nd	171	18	Kodak	Kodak	NNP
cord-048471-7jszm1nd	171	19	X	X	NNP
cord-048471-7jszm1nd	171	20	-	-	HYPH
cord-048471-7jszm1nd	171	21	Omat	Omat	NNP
cord-048471-7jszm1nd	171	22	scientific	scientific	JJ
cord-048471-7jszm1nd	171	23	imaging	imaging	NN
cord-048471-7jszm1nd	171	24	film	film	NN
cord-048471-7jszm1nd	171	25	(	(	-LRB-
cord-048471-7jszm1nd	171	26	Sigma	Sigma	NNP
cord-048471-7jszm1nd	171	27	)	)	-RRB-
cord-048471-7jszm1nd	171	28	at	at	IN
cord-048471-7jszm1nd	171	29	270uC	270uc	NN
cord-048471-7jszm1nd	171	30	for	for	IN
cord-048471-7jszm1nd	171	31	16	16	CD
cord-048471-7jszm1nd	171	32	hr	hr	NN
cord-048471-7jszm1nd	171	33	followed	follow	VBN
cord-048471-7jszm1nd	171	34	by	by	IN
cord-048471-7jszm1nd	171	35	developing	develop	VBG
cord-048471-7jszm1nd	171	36	using	use	VBG
cord-048471-7jszm1nd	171	37	a	a	DT
cord-048471-7jszm1nd	171	38	Kodak	Kodak	NNP
cord-048471-7jszm1nd	171	39	automated	automate	VBN
cord-048471-7jszm1nd	171	40	developer	developer	NN
cord-048471-7jszm1nd	171	41	.	.	.
cord-048471-7jszm1nd	172	1	Specific	specific	JJ
cord-048471-7jszm1nd	172	2	V5-tagged	v5-tagge	VBN
cord-048471-7jszm1nd	172	3	proteins	protein	NNS
cord-048471-7jszm1nd	172	4	synthesised	synthesise	VBN
cord-048471-7jszm1nd	172	5	by	by	IN
cord-048471-7jszm1nd	172	6	TNTH	TNTH	NNS
cord-048471-7jszm1nd	172	7	were	be	VBD
cord-048471-7jszm1nd	172	8	precipitated	precipitate	VBN
cord-048471-7jszm1nd	172	9	from	from	IN
cord-048471-7jszm1nd	172	10	5	5	CD
cord-048471-7jszm1nd	172	11	-	-	SYM
cord-048471-7jszm1nd	172	12	10	10	CD
cord-048471-7jszm1nd	172	13	ml	ml	NNS
cord-048471-7jszm1nd	172	14	of	of	IN
cord-048471-7jszm1nd	172	15	reaction	reaction	NN
cord-048471-7jszm1nd	172	16	product	product	NN
cord-048471-7jszm1nd	172	17	using	use	VBG
cord-048471-7jszm1nd	172	18	the	the	DT
cord-048471-7jszm1nd	172	19	anti	anti	JJ
cord-048471-7jszm1nd	172	20	-	-	JJ
cord-048471-7jszm1nd	172	21	V5	v5	JJ
cord-048471-7jszm1nd	172	22	monoclonal	monoclonal	JJ
cord-048471-7jszm1nd	172	23	antibody	antibody	NN
cord-048471-7jszm1nd	172	24	(	(	-LRB-
cord-048471-7jszm1nd	172	25	Invitrogen	Invitrogen	NNP
cord-048471-7jszm1nd	172	26	)	)	-RRB-
cord-048471-7jszm1nd	172	27	at	at	IN
cord-048471-7jszm1nd	172	28	the	the	DT
cord-048471-7jszm1nd	172	29	recommended	recommend	VBN
cord-048471-7jszm1nd	172	30	dilution	dilution	NN
cord-048471-7jszm1nd	172	31	in	in	IN
cord-048471-7jszm1nd	172	32	600	600	CD
cord-048471-7jszm1nd	172	33	ml	ml	NNS
cord-048471-7jszm1nd	172	34	of	of	IN
cord-048471-7jszm1nd	172	35	16	16	CD
cord-048471-7jszm1nd	172	36	RIPA	RIPA	NNP
cord-048471-7jszm1nd	172	37	buffer	buffer	NN
cord-048471-7jszm1nd	172	38	(	(	-LRB-
cord-048471-7jszm1nd	172	39	diluted	dilute	VBN
cord-048471-7jszm1nd	172	40	from	from	IN
cord-048471-7jszm1nd	172	41	10x	10x	CD
cord-048471-7jszm1nd	172	42	stock	stock	NN
cord-048471-7jszm1nd	172	43	:	:	:
cord-048471-7jszm1nd	172	44	10	10	CD
cord-048471-7jszm1nd	172	45	mM	mM	NNP
cord-048471-7jszm1nd	172	46	Tris	Tris	NNP
cord-048471-7jszm1nd	172	47	-	-	HYPH
cord-048471-7jszm1nd	172	48	HCl	HCl	NNP
cord-048471-7jszm1nd	172	49	(	(	-LRB-
cord-048471-7jszm1nd	172	50	pH	pH	NNP
cord-048471-7jszm1nd	172	51	7.5	7.5	CD
cord-048471-7jszm1nd	172	52	)	)	-RRB-
cord-048471-7jszm1nd	172	53	,	,	,
cord-048471-7jszm1nd	172	54	1	1	CD
cord-048471-7jszm1nd	172	55	mM	mm	CD
cord-048471-7jszm1nd	172	56	EDTA	EDTA	NNP
cord-048471-7jszm1nd	172	57	,	,	,
cord-048471-7jszm1nd	172	58	0.15	0.15	CD
cord-048471-7jszm1nd	172	59	mM	mm	CD
cord-048471-7jszm1nd	172	60	NaCl	nacl	NN
cord-048471-7jszm1nd	172	61	,	,	,
cord-048471-7jszm1nd	172	62	0.1	0.1	CD
cord-048471-7jszm1nd	172	63	%	%	NN
cord-048471-7jszm1nd	172	64	SDS	SDS	NNP
cord-048471-7jszm1nd	172	65	,	,	,
cord-048471-7jszm1nd	172	66	0.5	0.5	CD
cord-048471-7jszm1nd	172	67	%	%	NN
cord-048471-7jszm1nd	172	68	Empigen	Empigen	NNP
cord-048471-7jszm1nd	172	69	BB	BB	NNP
cord-048471-7jszm1nd	172	70	,	,	,
cord-048471-7jszm1nd	172	71	0.1	0.1	CD
cord-048471-7jszm1nd	172	72	mM	mm	CD
cord-048471-7jszm1nd	172	73	phenylmethylsulphonylfluoride	phenylmethylsulphonylfluoride	NN
cord-048471-7jszm1nd	172	74	)	)	-RRB-
cord-048471-7jszm1nd	172	75	for	for	IN
cord-048471-7jszm1nd	172	76	1	1	CD
cord-048471-7jszm1nd	172	77	hr	hr	NN
cord-048471-7jszm1nd	172	78	at	at	IN
cord-048471-7jszm1nd	172	79	37uC.	37uc.	CD
cord-048471-7jszm1nd	173	1	This	this	DT
cord-048471-7jszm1nd	173	2	was	be	VBD
cord-048471-7jszm1nd	173	3	followed	follow	VBN
cord-048471-7jszm1nd	173	4	by	by	IN
cord-048471-7jszm1nd	173	5	a	a	DT
cord-048471-7jszm1nd	173	6	second	second	JJ
cord-048471-7jszm1nd	173	7	incubation	incubation	NN
cord-048471-7jszm1nd	173	8	of	of	IN
cord-048471-7jszm1nd	173	9	tube	tube	NN
cord-048471-7jszm1nd	173	10	for	for	IN
cord-048471-7jszm1nd	173	11	2	2	CD
cord-048471-7jszm1nd	173	12	hr	hr	NN
cord-048471-7jszm1nd	173	13	rotating	rotate	VBG
cord-048471-7jszm1nd	173	14	at	at	IN
cord-048471-7jszm1nd	173	15	room	room	NN
cord-048471-7jszm1nd	173	16	temperature	temperature	NN
cord-048471-7jszm1nd	173	17	with	with	IN
cord-048471-7jszm1nd	173	18	goat	goat	NN
cord-048471-7jszm1nd	173	19	anti	anti	JJ
cord-048471-7jszm1nd	173	20	-	-	JJ
cord-048471-7jszm1nd	173	21	mouse	mouse	JJ
cord-048471-7jszm1nd	173	22	immunoglobulin	immunoglobulin	NN
cord-048471-7jszm1nd	173	23	G	g	NN
cord-048471-7jszm1nd	173	24	agarose	agarose	NN
cord-048471-7jszm1nd	173	25	beads	bead	NNS
cord-048471-7jszm1nd	173	26	(	(	-LRB-
cord-048471-7jszm1nd	173	27	Sigma	Sigma	NNP
cord-048471-7jszm1nd	173	28	)	)	-RRB-
cord-048471-7jszm1nd	173	29	to	to	TO
cord-048471-7jszm1nd	173	30	absorb	absorb	VB
cord-048471-7jszm1nd	173	31	the	the	DT
cord-048471-7jszm1nd	173	32	immune	immune	JJ
cord-048471-7jszm1nd	173	33	complexes	complex	NNS
cord-048471-7jszm1nd	173	34	.	.	.
cord-048471-7jszm1nd	174	1	The	the	DT
cord-048471-7jszm1nd	174	2	beads	bead	NNS
cord-048471-7jszm1nd	174	3	were	be	VBD
cord-048471-7jszm1nd	174	4	washed	wash	VBN
cord-048471-7jszm1nd	174	5	three	three	CD
cord-048471-7jszm1nd	174	6	times	time	NNS
cord-048471-7jszm1nd	174	7	with	with	IN
cord-048471-7jszm1nd	174	8	500	500	CD
cord-048471-7jszm1nd	174	9	ml	ml	NN
cord-048471-7jszm1nd	174	10	16	16	CD
cord-048471-7jszm1nd	174	11	RIPA	RIPA	NNP
cord-048471-7jszm1nd	174	12	buffer	buffer	NN
cord-048471-7jszm1nd	174	13	and	and	CC
cord-048471-7jszm1nd	174	14	once	once	RB
cord-048471-7jszm1nd	174	15	with	with	IN
cord-048471-7jszm1nd	174	16	500	500	CD
cord-048471-7jszm1nd	174	17	ml	ml	NN
cord-048471-7jszm1nd	174	18	PBS	PBS	NNP
cord-048471-7jszm1nd	174	19	.	.	.
cord-048471-7jszm1nd	175	1	The	the	DT
cord-048471-7jszm1nd	175	2	beads	bead	NNS
cord-048471-7jszm1nd	175	3	were	be	VBD
cord-048471-7jszm1nd	175	4	resuspended	resuspend	VBN
cord-048471-7jszm1nd	175	5	in	in	IN
cord-048471-7jszm1nd	175	6	sample	sample	NN
cord-048471-7jszm1nd	175	7	buffer	buffer	NN
cord-048471-7jszm1nd	175	8	for	for	IN
cord-048471-7jszm1nd	175	9	analysis	analysis	NN
cord-048471-7jszm1nd	175	10	by	by	IN
cord-048471-7jszm1nd	175	11	SDS	SDS	NNP
cord-048471-7jszm1nd	175	12	-	-	HYPH
cord-048471-7jszm1nd	175	13	PAGE	PAGE	NNP
cord-048471-7jszm1nd	175	14	and	and	CC
cord-048471-7jszm1nd	175	15	autoradiography	autoradiography	NN
cord-048471-7jszm1nd	175	16	as	as	IN
cord-048471-7jszm1nd	175	17	before	before	RB
cord-048471-7jszm1nd	175	18	.	.	.
cord-048471-7jszm1nd	176	1	Endotoxin	endotoxin	NN
cord-048471-7jszm1nd	176	2	-	-	HYPH
cord-048471-7jszm1nd	176	3	free	free	JJ
cord-048471-7jszm1nd	176	4	preparations	preparation	NNS
cord-048471-7jszm1nd	176	5	of	of	IN
cord-048471-7jszm1nd	176	6	plasmid	plasmid	NN
cord-048471-7jszm1nd	176	7	DNA	DNA	NNP
cord-048471-7jszm1nd	176	8	were	be	VBD
cord-048471-7jszm1nd	176	9	prepared	prepare	VBN
cord-048471-7jszm1nd	176	10	using	use	VBG
cord-048471-7jszm1nd	176	11	the	the	DT
cord-048471-7jszm1nd	176	12	GenElute	GenElute	NNP
cord-048471-7jszm1nd	176	13	TM	TM	NNP
cord-048471-7jszm1nd	176	14	Endotoxin	Endotoxin	NNP
cord-048471-7jszm1nd	176	15	free	free	JJ
cord-048471-7jszm1nd	176	16	plasmid	plasmid	NN
cord-048471-7jszm1nd	176	17	midi	midi	NNP
cord-048471-7jszm1nd	176	18	prep	prep	NNP
cord-048471-7jszm1nd	176	19	kit	kit	NNP
cord-048471-7jszm1nd	176	20	(	(	-LRB-
cord-048471-7jszm1nd	176	21	Sigma	Sigma	NNP
cord-048471-7jszm1nd	176	22	)	)	-RRB-
cord-048471-7jszm1nd	176	23	.	.	.
cord-048471-7jszm1nd	177	1	Crandall	Crandall	NNP
cord-048471-7jszm1nd	177	2	-	-	HYPH
cord-048471-7jszm1nd	177	3	Reese	Reese	NNP
cord-048471-7jszm1nd	177	4	Feline	Feline	NNP
cord-048471-7jszm1nd	177	5	Kidney	kidney	NN
cord-048471-7jszm1nd	177	6	cells	cell	NNS
cord-048471-7jszm1nd	177	7	(	(	-LRB-
cord-048471-7jszm1nd	177	8	CRFKs	crfk	NNS
cord-048471-7jszm1nd	177	9	)	)	-RRB-
cord-048471-7jszm1nd	177	10	were	be	VBD
cord-048471-7jszm1nd	177	11	seeded	seed	VBN
cord-048471-7jszm1nd	177	12	into	into	IN
cord-048471-7jszm1nd	177	13	a	a	DT
cord-048471-7jszm1nd	177	14	12	12	CD
cord-048471-7jszm1nd	177	15	well	well	NN
cord-048471-7jszm1nd	177	16	tray	tray	NN
cord-048471-7jszm1nd	177	17	at	at	IN
cord-048471-7jszm1nd	177	18	approximately	approximately	RB
cord-048471-7jszm1nd	177	19	40	40	CD
cord-048471-7jszm1nd	177	20	-	-	SYM
cord-048471-7jszm1nd	177	21	50	50	CD
cord-048471-7jszm1nd	177	22	%	%	NN
cord-048471-7jszm1nd	177	23	confluence	confluence	NN
cord-048471-7jszm1nd	177	24	.	.	.
cord-048471-7jszm1nd	178	1	CRFK	CRFK	NNP
cord-048471-7jszm1nd	178	2	cells	cell	NNS
cord-048471-7jszm1nd	178	3	were	be	VBD
cord-048471-7jszm1nd	178	4	transfected	transfecte	VBN
cord-048471-7jszm1nd	178	5	with	with	IN
cord-048471-7jszm1nd	178	6	no	no	DT
cord-048471-7jszm1nd	178	7	DNA	dna	NN
cord-048471-7jszm1nd	178	8	(	(	-LRB-
cord-048471-7jszm1nd	178	9	negative	negative	JJ
cord-048471-7jszm1nd	178	10	control	control	NN
cord-048471-7jszm1nd	178	11	)	)	-RRB-
cord-048471-7jszm1nd	178	12	,	,	,
cord-048471-7jszm1nd	178	13	pSV	pSV	NNP
cord-048471-7jszm1nd	178	14	-	-	HYPH
cord-048471-7jszm1nd	178	15	b	b	NNP
cord-048471-7jszm1nd	178	16	-	-	:
cord-048471-7jszm1nd	178	17	Gal	gal	NN
cord-048471-7jszm1nd	178	18	(	(	-LRB-
cord-048471-7jszm1nd	178	19	control	control	NN
cord-048471-7jszm1nd	178	20	for	for	IN
cord-048471-7jszm1nd	178	21	b	b	NN
cord-048471-7jszm1nd	178	22	-	-	HYPH
cord-048471-7jszm1nd	178	23	galactosidase	galactosidase	NN
cord-048471-7jszm1nd	178	24	activity	activity	NN
cord-048471-7jszm1nd	178	25	)	)	-RRB-
cord-048471-7jszm1nd	178	26	,	,	,
cord-048471-7jszm1nd	178	27	pEGFP	pEGFP	NNP
cord-048471-7jszm1nd	178	28	-	-	HYPH
cord-048471-7jszm1nd	178	29	C1	C1	NNP
cord-048471-7jszm1nd	178	30	/	/	SYM
cord-048471-7jszm1nd	178	31	JV	JV	NNP
cord-048471-7jszm1nd	178	32	59	59	CD
cord-048471-7jszm1nd	178	33	GS	GS	NNP
cord-048471-7jszm1nd	178	34	/	/	SYM
cord-048471-7jszm1nd	178	35	lacZ	lacZ	NNP
cord-048471-7jszm1nd	178	36	and	and	CC
cord-048471-7jszm1nd	178	37	pEGFP	pEGFP	NNP
cord-048471-7jszm1nd	178	38	-	-	HYPH
cord-048471-7jszm1nd	178	39	C1	C1	NNP
cord-048471-7jszm1nd	178	40	/	/	SYM
cord-048471-7jszm1nd	178	41	IRES	IRES	NNP
cord-048471-7jszm1nd	178	42	/	/	SYM
cord-048471-7jszm1nd	178	43	lacZ	lacz	XX
cord-048471-7jszm1nd	179	1	(	(	-LRB-
cord-048471-7jszm1nd	179	2	control	control	NN
cord-048471-7jszm1nd	179	3	for	for	IN
cord-048471-7jszm1nd	179	4	IRES	ires	JJ
cord-048471-7jszm1nd	179	5	activity	activity	NN
cord-048471-7jszm1nd	179	6	)	)	-RRB-
cord-048471-7jszm1nd	179	7	using	use	VBG
cord-048471-7jszm1nd	179	8	the	the	DT
cord-048471-7jszm1nd	179	9	Superfect	Superfect	NNP
cord-048471-7jszm1nd	179	10	TM	TM	NNP
cord-048471-7jszm1nd	179	11	transfection	transfection	NN
cord-048471-7jszm1nd	179	12	reagent	reagent	NN
cord-048471-7jszm1nd	179	13	(	(	-LRB-
cord-048471-7jszm1nd	179	14	Qiagen	Qiagen	NNP
cord-048471-7jszm1nd	179	15	)	)	-RRB-
cord-048471-7jszm1nd	179	16	as	as	IN
cord-048471-7jszm1nd	179	17	per	per	IN
cord-048471-7jszm1nd	179	18	the	the	DT
cord-048471-7jszm1nd	179	19	manufacturer	manufacturer	NN
cord-048471-7jszm1nd	179	20	's	's	POS
cord-048471-7jszm1nd	179	21	recommendations	recommendation	NNS
cord-048471-7jszm1nd	179	22	.	.	.
cord-048471-7jszm1nd	180	1	Following	follow	VBG
cord-048471-7jszm1nd	180	2	a	a	DT
cord-048471-7jszm1nd	180	3	16	16	CD
cord-048471-7jszm1nd	180	4	hour	hour	NN
cord-048471-7jszm1nd	180	5	incubation	incubation	NN
cord-048471-7jszm1nd	180	6	the	the	DT
cord-048471-7jszm1nd	180	7	cells	cell	NNS
cord-048471-7jszm1nd	180	8	were	be	VBD
cord-048471-7jszm1nd	180	9	observed	observe	VBN
cord-048471-7jszm1nd	180	10	for	for	IN
cord-048471-7jszm1nd	180	11	EGFP	EGFP	NNP
cord-048471-7jszm1nd	180	12	expression	expression	NN
cord-048471-7jszm1nd	180	13	using	use	VBG
cord-048471-7jszm1nd	180	14	a	a	DT
cord-048471-7jszm1nd	180	15	Leica	Leica	NNP
cord-048471-7jszm1nd	180	16	Leitz	Leitz	NNP
cord-048471-7jszm1nd	180	17	DMRB	DMRB	NNP
cord-048471-7jszm1nd	180	18	fluorescence	fluorescence	NN
cord-048471-7jszm1nd	180	19	microscope	microscope	NN
cord-048471-7jszm1nd	180	20	.	.	.
cord-048471-7jszm1nd	181	1	The	the	DT
cord-048471-7jszm1nd	181	2	cells	cell	NNS
cord-048471-7jszm1nd	181	3	were	be	VBD
cord-048471-7jszm1nd	181	4	washed	wash	VBN
cord-048471-7jszm1nd	181	5	in	in	IN
cord-048471-7jszm1nd	181	6	PBS	PBS	NNP
cord-048471-7jszm1nd	181	7	and	and	CC
cord-048471-7jszm1nd	181	8	fixed	fix	VBN
cord-048471-7jszm1nd	181	9	using	use	VBG
cord-048471-7jszm1nd	181	10	a	a	DT
cord-048471-7jszm1nd	181	11	0.5	0.5	CD
cord-048471-7jszm1nd	181	12	%	%	NN
cord-048471-7jszm1nd	181	13	solution	solution	NN
cord-048471-7jszm1nd	181	14	of	of	IN
cord-048471-7jszm1nd	181	15	glutaraldehyde	glutaraldehyde	NN
cord-048471-7jszm1nd	181	16	for	for	IN
cord-048471-7jszm1nd	181	17	30	30	CD
cord-048471-7jszm1nd	181	18	min	min	NN
cord-048471-7jszm1nd	181	19	at	at	IN
cord-048471-7jszm1nd	181	20	room	room	NN
cord-048471-7jszm1nd	181	21	temperature	temperature	NN
cord-048471-7jszm1nd	181	22	.	.	.
cord-048471-7jszm1nd	182	1	The	the	DT
cord-048471-7jszm1nd	182	2	cells	cell	NNS
cord-048471-7jszm1nd	182	3	were	be	VBD
cord-048471-7jszm1nd	182	4	incubated	incubate	VBN
cord-048471-7jszm1nd	182	5	with	with	IN
cord-048471-7jszm1nd	182	6	an	an	DT
cord-048471-7jszm1nd	182	7	X	X	NNP
cord-048471-7jszm1nd	182	8	-	-	HYPH
cord-048471-7jszm1nd	182	9	Gal	gal	NN
cord-048471-7jszm1nd	182	10	stain	stain	NN
cord-048471-7jszm1nd	182	11	solution	solution	NN
cord-048471-7jszm1nd	182	12	:	:	:
cord-048471-7jszm1nd	182	13	5	5	CD
cord-048471-7jszm1nd	182	14	mM	mm	CD
cord-048471-7jszm1nd	182	15	K	k	NN
cord-048471-7jszm1nd	182	16	3	3	CD
cord-048471-7jszm1nd	182	17	Fe(CN	fe(cn	NN
cord-048471-7jszm1nd	182	18	)	)	-RRB-
cord-048471-7jszm1nd	182	19	6	6	CD
cord-048471-7jszm1nd	182	20	,	,	,
cord-048471-7jszm1nd	182	21	5	5	CD
cord-048471-7jszm1nd	182	22	mM	mm	CD
cord-048471-7jszm1nd	182	23	K	k	NN
cord-048471-7jszm1nd	182	24	4	4	CD
cord-048471-7jszm1nd	182	25	Fe(CN	fe(cn	NN
cord-048471-7jszm1nd	182	26	)	)	-RRB-
cord-048471-7jszm1nd	182	27	6	6	CD
cord-048471-7jszm1nd	182	28	,	,	,
cord-048471-7jszm1nd	182	29	2	2	CD
cord-048471-7jszm1nd	182	30	mM	mm	CD
cord-048471-7jszm1nd	182	31	MgCl	MgCl	NNP
cord-048471-7jszm1nd	182	32	2	2	CD
cord-048471-7jszm1nd	182	33	,	,	,
cord-048471-7jszm1nd	182	34	1x	1x	CD
cord-048471-7jszm1nd	182	35	X	x	NN
cord-048471-7jszm1nd	182	36	-	-	NN
cord-048471-7jszm1nd	182	37	Gal	Gal	NNP
cord-048471-7jszm1nd	182	38	(	(	-LRB-
cord-048471-7jszm1nd	182	39	Sigma	Sigma	NNP
cord-048471-7jszm1nd	182	40	)	)	-RRB-
cord-048471-7jszm1nd	182	41	for	for	IN
cord-048471-7jszm1nd	182	42	4	4	CD
cord-048471-7jszm1nd	182	43	hours	hour	NNS
cord-048471-7jszm1nd	182	44	at	at	IN
cord-048471-7jszm1nd	182	45	37uC	37uc	CD
cord-048471-7jszm1nd	182	46	and	and	CC
cord-048471-7jszm1nd	182	47	were	be	VBD
cord-048471-7jszm1nd	182	48	observed	observe	VBN
cord-048471-7jszm1nd	182	49	for	for	IN
cord-048471-7jszm1nd	182	50	b	b	NN
cord-048471-7jszm1nd	182	51	-	-	HYPH
cord-048471-7jszm1nd	182	52	glactosidase	glactosidase	JJ
cord-048471-7jszm1nd	182	53	activity	activity	NN
cord-048471-7jszm1nd	182	54	by	by	IN
cord-048471-7jszm1nd	182	55	light	light	JJ
cord-048471-7jszm1nd	182	56	microscopy	microscopy	NNS
cord-048471-7jszm1nd	182	57	.	.	.
cord-048471-7jszm1nd	183	1	The	the	DT
cord-048471-7jszm1nd	183	2	experiment	experiment	NN
cord-048471-7jszm1nd	183	3	was	be	VBD
cord-048471-7jszm1nd	183	4	performed	perform	VBN
cord-048471-7jszm1nd	183	5	more	more	RBR
cord-048471-7jszm1nd	183	6	than	than	IN
cord-048471-7jszm1nd	183	7	once	once	RB
cord-048471-7jszm1nd	183	8	to	to	TO
cord-048471-7jszm1nd	183	9	confirm	confirm	VB
cord-048471-7jszm1nd	183	10	the	the	DT
cord-048471-7jszm1nd	183	11	results	result	NNS
cord-048471-7jszm1nd	183	12	.	.	.
cord-048471-7jszm1nd	184	1	JV	JV	NNP
cord-048471-7jszm1nd	184	2	V5	V5	NNP
cord-048471-7jszm1nd	184	3	and	and	CC
cord-048471-7jszm1nd	184	4	JV	JV	NNP
cord-048471-7jszm1nd	184	5	FLC	FLC	NNP
cord-048471-7jszm1nd	184	6	T7	T7	NNP
cord-048471-7jszm1nd	184	7	cDNA	cdna	NN
cord-048471-7jszm1nd	184	8	plasmid	plasmid	NN
cord-048471-7jszm1nd	184	9	constructs	construct	NNS
cord-048471-7jszm1nd	184	10	were	be	VBD
cord-048471-7jszm1nd	184	11	linearised	linearise	VBN
cord-048471-7jszm1nd	184	12	using	use	VBG
cord-048471-7jszm1nd	184	13	NdeI	NdeI	NNP
cord-048471-7jszm1nd	184	14	(	(	-LRB-
cord-048471-7jszm1nd	184	15	Invitrogen	Invitrogen	NNP
cord-048471-7jszm1nd	184	16	)	)	-RRB-
cord-048471-7jszm1nd	184	17	.	.	.
cord-048471-7jszm1nd	185	1	Capped	Capped	NNP
cord-048471-7jszm1nd	185	2	RNA	RNA	NNP
cord-048471-7jszm1nd	185	3	was	be	VBD
cord-048471-7jszm1nd	185	4	synthesised	synthesise	VBN
cord-048471-7jszm1nd	185	5	using	use	VBG
cord-048471-7jszm1nd	185	6	the	the	DT
cord-048471-7jszm1nd	185	7	mMessage	mMessage	NNP
cord-048471-7jszm1nd	185	8	mMachineH	mMachineH	HYPH
cord-048471-7jszm1nd	185	9	Capped	cap	VBN
cord-048471-7jszm1nd	185	10	RNA	RNA	NNP
cord-048471-7jszm1nd	185	11	Transcription	Transcription	NNP
cord-048471-7jszm1nd	185	12	kit	kit	NN
cord-048471-7jszm1nd	185	13	(	(	-LRB-
cord-048471-7jszm1nd	185	14	Ambion	Ambion	NNP
cord-048471-7jszm1nd	185	15	)	)	-RRB-
cord-048471-7jszm1nd	185	16	according	accord	VBG
cord-048471-7jszm1nd	185	17	to	to	IN
cord-048471-7jszm1nd	185	18	the	the	DT
cord-048471-7jszm1nd	185	19	manufacturer	manufacturer	NN
cord-048471-7jszm1nd	185	20	's	's	POS
cord-048471-7jszm1nd	185	21	instructions	instruction	NNS
cord-048471-7jszm1nd	185	22	.	.	.
cord-048471-7jszm1nd	186	1	CRFK	CRFK	NNP
cord-048471-7jszm1nd	186	2	cells	cell	NNS
cord-048471-7jszm1nd	186	3	were	be	VBD
cord-048471-7jszm1nd	186	4	seeded	seed	VBN
cord-048471-7jszm1nd	186	5	into	into	IN
cord-048471-7jszm1nd	186	6	6	6	CD
cord-048471-7jszm1nd	186	7	well	well	NN
cord-048471-7jszm1nd	186	8	trays	tray	NNS
cord-048471-7jszm1nd	186	9	at	at	IN
cord-048471-7jszm1nd	186	10	approximately	approximately	RB
cord-048471-7jszm1nd	186	11	50	50	CD
cord-048471-7jszm1nd	186	12	%	%	NN
cord-048471-7jszm1nd	186	13	confluence	confluence	NN
cord-048471-7jszm1nd	186	14	and	and	CC
cord-048471-7jszm1nd	186	15	were	be	VBD
cord-048471-7jszm1nd	186	16	transfected	transfecte	VBN
cord-048471-7jszm1nd	186	17	with	with	IN
cord-048471-7jszm1nd	186	18	2	2	CD
cord-048471-7jszm1nd	186	19	mg	mg	NNP
cord-048471-7jszm1nd	186	20	purified	purify	VBN
cord-048471-7jszm1nd	186	21	RNA	RNA	NNP
cord-048471-7jszm1nd	186	22	per	per	IN
cord-048471-7jszm1nd	186	23	well	well	RB
cord-048471-7jszm1nd	186	24	using	use	VBG
cord-048471-7jszm1nd	186	25	Transmessenger	Transmessenger	NNP
cord-048471-7jszm1nd	186	26	transfection	transfection	NN
cord-048471-7jszm1nd	186	27	reagent	reagent	NN
cord-048471-7jszm1nd	186	28	(	(	-LRB-
cord-048471-7jszm1nd	186	29	Qiagen	Qiagen	NNP
cord-048471-7jszm1nd	186	30	)	)	-RRB-
cord-048471-7jszm1nd	186	31	according	accord	VBG
cord-048471-7jszm1nd	186	32	to	to	IN
cord-048471-7jszm1nd	186	33	the	the	DT
cord-048471-7jszm1nd	186	34	manufacturer	manufacturer	NN
cord-048471-7jszm1nd	186	35	's	's	POS
cord-048471-7jszm1nd	186	36	instructions	instruction	NNS
cord-048471-7jszm1nd	186	37	.	.	.
cord-048471-7jszm1nd	187	1	For	for	IN
cord-048471-7jszm1nd	187	2	immunofluorescence	immunofluorescence	NN
cord-048471-7jszm1nd	187	3	CRFK	CRFK	NNP
cord-048471-7jszm1nd	187	4	cells	cell	NNS
cord-048471-7jszm1nd	187	5	were	be	VBD
cord-048471-7jszm1nd	187	6	seeded	seed	VBN
cord-048471-7jszm1nd	187	7	onto	onto	IN
cord-048471-7jszm1nd	187	8	19	19	CD
cord-048471-7jszm1nd	187	9	mm	mm	NN
cord-048471-7jszm1nd	187	10	coverslips	coverslip	NNS
cord-048471-7jszm1nd	187	11	in	in	IN
cord-048471-7jszm1nd	187	12	6	6	CD
cord-048471-7jszm1nd	187	13	well	well	NN
cord-048471-7jszm1nd	187	14	trays	tray	NNS
cord-048471-7jszm1nd	187	15	and	and	CC
cord-048471-7jszm1nd	187	16	were	be	VBD
cord-048471-7jszm1nd	187	17	transfected	transfecte	VBN
cord-048471-7jszm1nd	187	18	with	with	IN
cord-048471-7jszm1nd	187	19	RNA	RNA	NNP
cord-048471-7jszm1nd	187	20	as	as	IN
cord-048471-7jszm1nd	187	21	described	describe	VBN
cord-048471-7jszm1nd	187	22	.	.	.
cord-048471-7jszm1nd	188	1	Following	follow	VBG
cord-048471-7jszm1nd	188	2	a	a	DT
cord-048471-7jszm1nd	188	3	24	24	CD
cord-048471-7jszm1nd	188	4	hr	hr	NN
cord-048471-7jszm1nd	188	5	incubation	incubation	NN
cord-048471-7jszm1nd	188	6	the	the	DT
cord-048471-7jszm1nd	188	7	coverslips	coverslip	NNS
cord-048471-7jszm1nd	188	8	were	be	VBD
cord-048471-7jszm1nd	188	9	washed	wash	VBN
cord-048471-7jszm1nd	188	10	with	with	IN
cord-048471-7jszm1nd	188	11	PBS	PBS	NNP
cord-048471-7jszm1nd	188	12	and	and	CC
cord-048471-7jszm1nd	188	13	fixed	fix	VBN
cord-048471-7jszm1nd	188	14	in	in	IN
cord-048471-7jszm1nd	188	15	4	4	CD
cord-048471-7jszm1nd	188	16	%	%	NN
cord-048471-7jszm1nd	188	17	formaldehyde	formaldehyde	NN
cord-048471-7jszm1nd	188	18	for	for	IN
cord-048471-7jszm1nd	188	19	15	15	CD
cord-048471-7jszm1nd	188	20	min	min	NN
cord-048471-7jszm1nd	188	21	at	at	IN
cord-048471-7jszm1nd	188	22	room	room	NN
cord-048471-7jszm1nd	188	23	temperature	temperature	NN
cord-048471-7jszm1nd	188	24	.	.	.
cord-048471-7jszm1nd	189	1	Cells	cell	NNS
cord-048471-7jszm1nd	189	2	were	be	VBD
cord-048471-7jszm1nd	189	3	permeabilised	permeabilise	VBN
cord-048471-7jszm1nd	189	4	and	and	CC
cord-048471-7jszm1nd	189	5	blocked	block	VBN
cord-048471-7jszm1nd	189	6	in	in	IN
cord-048471-7jszm1nd	189	7	saponin	saponin	NN
cord-048471-7jszm1nd	189	8	buffer	buffer	NN
cord-048471-7jszm1nd	189	9	,	,	,
cord-048471-7jszm1nd	189	10	also	also	RB
cord-048471-7jszm1nd	189	11	used	use	VBN
cord-048471-7jszm1nd	189	12	as	as	IN
cord-048471-7jszm1nd	189	13	staining	stain	VBG
cord-048471-7jszm1nd	189	14	buffer	buffer	NN
cord-048471-7jszm1nd	189	15	,	,	,
cord-048471-7jszm1nd	189	16	(	(	-LRB-
cord-048471-7jszm1nd	189	17	0.1	0.1	CD
cord-048471-7jszm1nd	189	18	%	%	NN
cord-048471-7jszm1nd	189	19	saponin	saponin	NN
cord-048471-7jszm1nd	189	20	,	,	,
cord-048471-7jszm1nd	189	21	10	10	CD
cord-048471-7jszm1nd	189	22	%	%	NN
cord-048471-7jszm1nd	189	23	foetal	foetal	JJ
cord-048471-7jszm1nd	189	24	calf	calf	NN
cord-048471-7jszm1nd	189	25	serum	serum	NN
cord-048471-7jszm1nd	189	26	,	,	,
cord-048471-7jszm1nd	189	27	0.1	0.1	CD
cord-048471-7jszm1nd	189	28	%	%	NN
cord-048471-7jszm1nd	189	29	sodium	sodium	NN
cord-048471-7jszm1nd	189	30	azide	azide	NN
cord-048471-7jszm1nd	189	31	)	)	-RRB-
cord-048471-7jszm1nd	189	32	for	for	IN
cord-048471-7jszm1nd	189	33	1	1	CD
cord-048471-7jszm1nd	189	34	hr	hr	NN
cord-048471-7jszm1nd	189	35	at	at	IN
cord-048471-7jszm1nd	189	36	4uC.	4uc.	CD
cord-048471-7jszm1nd	189	37	Cells	cell	NNS
cord-048471-7jszm1nd	189	38	were	be	VBD
cord-048471-7jszm1nd	189	39	stained	stain	VBN
cord-048471-7jszm1nd	189	40	using	use	VBG
cord-048471-7jszm1nd	189	41	an	an	DT
cord-048471-7jszm1nd	189	42	anti	anti	JJ
cord-048471-7jszm1nd	189	43	-	-	JJ
cord-048471-7jszm1nd	189	44	V5	v5	JJ
cord-048471-7jszm1nd	189	45	monoclonal	monoclonal	JJ
cord-048471-7jszm1nd	189	46	antibody	antibody	NN
cord-048471-7jszm1nd	189	47	(	(	-LRB-
cord-048471-7jszm1nd	189	48	Invitrogen	Invitrogen	NNP
cord-048471-7jszm1nd	189	49	)	)	-RRB-
cord-048471-7jszm1nd	189	50	followed	follow	VBN
cord-048471-7jszm1nd	189	51	by	by	IN
cord-048471-7jszm1nd	189	52	an	an	DT
cord-048471-7jszm1nd	189	53	anti	anti	JJ
cord-048471-7jszm1nd	189	54	-	-	JJ
cord-048471-7jszm1nd	189	55	mouse	mouse	NN
cord-048471-7jszm1nd	189	56	Alexafluor	Alexafluor	NNP
cord-048471-7jszm1nd	189	57	488	488	CD
cord-048471-7jszm1nd	189	58	conjugated	conjugate	VBD
cord-048471-7jszm1nd	189	59	secondary	secondary	JJ
cord-048471-7jszm1nd	189	60	antibody	antibody	NN
cord-048471-7jszm1nd	189	61	(	(	-LRB-
cord-048471-7jszm1nd	189	62	Molecular	Molecular	NNP
cord-048471-7jszm1nd	189	63	Probes	Probes	NNPS
cord-048471-7jszm1nd	189	64	)	)	-RRB-
cord-048471-7jszm1nd	189	65	at	at	IN
cord-048471-7jszm1nd	189	66	the	the	DT
cord-048471-7jszm1nd	189	67	recommended	recommend	VBN
cord-048471-7jszm1nd	189	68	dilution	dilution	NN
cord-048471-7jszm1nd	189	69	in	in	IN
cord-048471-7jszm1nd	189	70	staining	stain	VBG
cord-048471-7jszm1nd	189	71	buffer	buffer	NN
cord-048471-7jszm1nd	189	72	for	for	IN
cord-048471-7jszm1nd	189	73	30	30	CD
cord-048471-7jszm1nd	189	74	min	min	NN
cord-048471-7jszm1nd	189	75	in	in	IN
cord-048471-7jszm1nd	189	76	the	the	DT
cord-048471-7jszm1nd	189	77	dark	dark	NN
cord-048471-7jszm1nd	189	78	.	.	.
cord-048471-7jszm1nd	190	1	Cells	cell	NNS
cord-048471-7jszm1nd	190	2	were	be	VBD
cord-048471-7jszm1nd	190	3	then	then	RB
cord-048471-7jszm1nd	190	4	stained	stain	VBN
cord-048471-7jszm1nd	190	5	for	for	IN
cord-048471-7jszm1nd	190	6	30	30	CD
cord-048471-7jszm1nd	190	7	min	min	NN
cord-048471-7jszm1nd	190	8	in	in	IN
cord-048471-7jszm1nd	190	9	the	the	DT
cord-048471-7jszm1nd	190	10	dark	dark	NN
cord-048471-7jszm1nd	190	11	with	with	IN
cord-048471-7jszm1nd	190	12	a	a	DT
cord-048471-7jszm1nd	190	13	Wheat	Wheat	NNP
cord-048471-7jszm1nd	190	14	Germ	Germ	NNP
cord-048471-7jszm1nd	190	15	Agglutinin	Agglutinin	NNP
cord-048471-7jszm1nd	190	16	Alexafluor	Alexafluor	NNP
cord-048471-7jszm1nd	190	17	594	594	CD
cord-048471-7jszm1nd	190	18	nm	nm	NN
cord-048471-7jszm1nd	190	19	conjugate	conjugate	NNP
cord-048471-7jszm1nd	190	20	(	(	-LRB-
cord-048471-7jszm1nd	190	21	Molcular	Molcular	NNP
cord-048471-7jszm1nd	190	22	Probes	Probes	NNPS
cord-048471-7jszm1nd	190	23	)	)	-RRB-
cord-048471-7jszm1nd	190	24	to	to	TO
cord-048471-7jszm1nd	190	25	allow	allow	VB
cord-048471-7jszm1nd	190	26	identification	identification	NN
cord-048471-7jszm1nd	190	27	of	of	IN
cord-048471-7jszm1nd	190	28	plasma	plasma	NN
cord-048471-7jszm1nd	190	29	and	and	CC
cord-048471-7jszm1nd	190	30	Golgi	Golgi	NNP
cord-048471-7jszm1nd	190	31	membranes	membrane	NNS
cord-048471-7jszm1nd	190	32	.	.	.
cord-048471-7jszm1nd	191	1	Coverslips	coverslip	NNS
cord-048471-7jszm1nd	191	2	were	be	VBD
cord-048471-7jszm1nd	191	3	washed	wash	VBN
cord-048471-7jszm1nd	191	4	and	and	CC
cord-048471-7jszm1nd	191	5	mounted	mount	VBN
cord-048471-7jszm1nd	191	6	onto	onto	IN
cord-048471-7jszm1nd	191	7	slides	slide	NNS
cord-048471-7jszm1nd	191	8	using	use	VBG
cord-048471-7jszm1nd	191	9	Vectashield	Vectashield	NNP
cord-048471-7jszm1nd	191	10	containing	contain	VBG
cord-048471-7jszm1nd	191	11	DAPI	DAPI	NNP
cord-048471-7jszm1nd	191	12	(	(	-LRB-
cord-048471-7jszm1nd	191	13	Vector	Vector	NNP
cord-048471-7jszm1nd	191	14	Labs	Labs	NNP
cord-048471-7jszm1nd	191	15	)	)	-RRB-
cord-048471-7jszm1nd	191	16	.	.	.
cord-048471-7jszm1nd	192	1	Microscopy	Microscopy	NNP
cord-048471-7jszm1nd	192	2	was	be	VBD
cord-048471-7jszm1nd	192	3	performed	perform	VBN
cord-048471-7jszm1nd	192	4	using	use	VBG
cord-048471-7jszm1nd	192	5	an	an	DT
cord-048471-7jszm1nd	192	6	inverted	inverted	JJ
cord-048471-7jszm1nd	192	7	Leica	Leica	NNP
cord-048471-7jszm1nd	192	8	TCS	TCS	NNP
cord-048471-7jszm1nd	192	9	-	-	HYPH
cord-048471-7jszm1nd	192	10	NT	NT	NNP
cord-048471-7jszm1nd	192	11	confocal	confocal	JJ
cord-048471-7jszm1nd	192	12	laser	laser	NN
cord-048471-7jszm1nd	192	13	scanning	scanning	NN
cord-048471-7jszm1nd	192	14	microscope	microscope	NN
cord-048471-7jszm1nd	192	15	.	.	.
cord-048471-7jszm1nd	193	1	The	the	DT
cord-048471-7jszm1nd	193	2	anti	anti	JJ
cord-048471-7jszm1nd	193	3	-	-	JJ
cord-048471-7jszm1nd	193	4	V5	v5	JJ
cord-048471-7jszm1nd	193	5	antibody	antibody	NN
cord-048471-7jszm1nd	193	6	was	be	VBD
cord-048471-7jszm1nd	193	7	also	also	RB
cord-048471-7jszm1nd	193	8	used	use	VBN
cord-048471-7jszm1nd	193	9	to	to	TO
cord-048471-7jszm1nd	193	10	detect	detect	VB
cord-048471-7jszm1nd	193	11	V5-tagged	v5-tagged	JJ
cord-048471-7jszm1nd	193	12	protein	protein	NN
cord-048471-7jszm1nd	193	13	by	by	IN
cord-048471-7jszm1nd	193	14	Western	western	JJ
cord-048471-7jszm1nd	193	15	blot	blot	NN
cord-048471-7jszm1nd	193	16	.	.	.
cord-048471-7jszm1nd	194	1	Cell	cell	NN
cord-048471-7jszm1nd	194	2	lysates	lysate	NNS
cord-048471-7jszm1nd	194	3	were	be	VBD
cord-048471-7jszm1nd	194	4	prepared	prepare	VBN
cord-048471-7jszm1nd	194	5	following	follow	VBG
cord-048471-7jszm1nd	194	6	transfection	transfection	NN
cord-048471-7jszm1nd	194	7	using	use	VBG
cord-048471-7jszm1nd	194	8	lysis	lysis	NN
cord-048471-7jszm1nd	194	9	buffer	buffer	NN
cord-048471-7jszm1nd	194	10	(	(	-LRB-
cord-048471-7jszm1nd	194	11	0.15	0.15	CD
cord-048471-7jszm1nd	194	12	M	m	NN
cord-048471-7jszm1nd	194	13	sodium	sodium	NN
cord-048471-7jszm1nd	194	14	chloride	chloride	NN
cord-048471-7jszm1nd	194	15	,	,	,
cord-048471-7jszm1nd	194	16	0.5	0.5	CD
cord-048471-7jszm1nd	194	17	%	%	NN
cord-048471-7jszm1nd	194	18	(	(	-LRB-
cord-048471-7jszm1nd	194	19	v	v	NN
cord-048471-7jszm1nd	194	20	/	/	SYM
cord-048471-7jszm1nd	194	21	v	v	NN
cord-048471-7jszm1nd	194	22	)	)	-RRB-
cord-048471-7jszm1nd	194	23	sodium	sodium	NN
cord-048471-7jszm1nd	194	24	deoxycholate	deoxycholate	NN
cord-048471-7jszm1nd	194	25	,	,	,
cord-048471-7jszm1nd	194	26	0.1	0.1	CD
cord-048471-7jszm1nd	194	27	%	%	NN
cord-048471-7jszm1nd	194	28	(	(	-LRB-
cord-048471-7jszm1nd	194	29	w	w	NN
cord-048471-7jszm1nd	194	30	/	/	SYM
cord-048471-7jszm1nd	194	31	v	v	NN
cord-048471-7jszm1nd	194	32	)	)	-RRB-
cord-048471-7jszm1nd	194	33	SDS	SDS	NNP
cord-048471-7jszm1nd	194	34	,	,	,
cord-048471-7jszm1nd	194	35	50	50	CD
cord-048471-7jszm1nd	194	36	mM	mM	NNP
cord-048471-7jszm1nd	194	37	Tris	Tris	NNP
cord-048471-7jszm1nd	194	38	-	-	HYPH
cord-048471-7jszm1nd	194	39	Cl	Cl	NNP
cord-048471-7jszm1nd	194	40	pH	pH	NNP
cord-048471-7jszm1nd	194	41	8.0	8.0	CD
cord-048471-7jszm1nd	194	42	)	)	-RRB-
cord-048471-7jszm1nd	194	43	and	and	CC
cord-048471-7jszm1nd	194	44	protease	protease	JJ
cord-048471-7jszm1nd	194	45	inhibitor	inhibitor	NN
cord-048471-7jszm1nd	194	46	cocktail	cocktail	NN
cord-048471-7jszm1nd	194	47	(	(	-LRB-
cord-048471-7jszm1nd	194	48	Sigma	Sigma	NNP
cord-048471-7jszm1nd	194	49	)	)	-RRB-
cord-048471-7jszm1nd	194	50	.	.	.
cord-048471-7jszm1nd	195	1	Lysates	Lysates	NNP
cord-048471-7jszm1nd	195	2	were	be	VBD
cord-048471-7jszm1nd	195	3	incubated	incubate	VBN
cord-048471-7jszm1nd	195	4	for	for	IN
cord-048471-7jszm1nd	195	5	15	15	CD
cord-048471-7jszm1nd	195	6	min	min	NN
cord-048471-7jszm1nd	195	7	on	on	IN
cord-048471-7jszm1nd	195	8	ice	ice	NN
cord-048471-7jszm1nd	195	9	followed	follow	VBN
cord-048471-7jszm1nd	195	10	by	by	IN
cord-048471-7jszm1nd	195	11	sonication	sonication	NN
cord-048471-7jszm1nd	195	12	to	to	TO
cord-048471-7jszm1nd	195	13	shear	shear	VB
cord-048471-7jszm1nd	195	14	genomic	genomic	JJ
cord-048471-7jszm1nd	195	15	DNA	dna	NN
cord-048471-7jszm1nd	195	16	.	.	.
cord-048471-7jszm1nd	196	1	Following	follow	VBG
cord-048471-7jszm1nd	196	2	Bradford	Bradford	NNP
cord-048471-7jszm1nd	196	3	analysis	analysis	NN
cord-048471-7jszm1nd	196	4	equal	equal	VBP
cord-048471-7jszm1nd	196	5	protein	protein	NN
cord-048471-7jszm1nd	196	6	content	content	NN
cord-048471-7jszm1nd	196	7	from	from	IN
cord-048471-7jszm1nd	196	8	JV	JV	NNP
cord-048471-7jszm1nd	196	9	V5	V5	NNP
cord-048471-7jszm1nd	196	10	and	and	CC
cord-048471-7jszm1nd	196	11	JV	JV	NNP
cord-048471-7jszm1nd	196	12	FLC	FLC	NNP
cord-048471-7jszm1nd	196	13	lysates	lysate	NNS
cord-048471-7jszm1nd	196	14	were	be	VBD
cord-048471-7jszm1nd	196	15	run	run	VBN
cord-048471-7jszm1nd	196	16	on	on	IN
cord-048471-7jszm1nd	196	17	a	a	DT
cord-048471-7jszm1nd	196	18	10	10	CD
cord-048471-7jszm1nd	196	19	%	%	NN
cord-048471-7jszm1nd	196	20	SDS	sds	NN
cord-048471-7jszm1nd	196	21	-	-	HYPH
cord-048471-7jszm1nd	196	22	PAGE	PAGE	NNP
cord-048471-7jszm1nd	196	23	gel	gel	NN
cord-048471-7jszm1nd	196	24	and	and	CC
cord-048471-7jszm1nd	196	25	subsequently	subsequently	RB
cord-048471-7jszm1nd	196	26	transferred	transfer	VBD
cord-048471-7jszm1nd	196	27	onto	onto	IN
cord-048471-7jszm1nd	196	28	Immobilon	Immobilon	NNP
cord-048471-7jszm1nd	196	29	-	-	HYPH
cord-048471-7jszm1nd	196	30	P	p	NN
cord-048471-7jszm1nd	196	31	PVDF	pvdf	NN
cord-048471-7jszm1nd	196	32	membrane	membrane	NN
cord-048471-7jszm1nd	196	33	(	(	-LRB-
cord-048471-7jszm1nd	196	34	Millipore	Millipore	NNP
cord-048471-7jszm1nd	196	35	)	)	-RRB-
cord-048471-7jszm1nd	196	36	according	accord	VBG
cord-048471-7jszm1nd	196	37	to	to	IN
cord-048471-7jszm1nd	196	38	the	the	DT
cord-048471-7jszm1nd	196	39	manufacturer	manufacturer	NN
cord-048471-7jszm1nd	196	40	's	's	POS
cord-048471-7jszm1nd	196	41	recommendations	recommendation	NNS
cord-048471-7jszm1nd	196	42	.	.	.
cord-048471-7jszm1nd	197	1	The	the	DT
cord-048471-7jszm1nd	197	2	membrane	membrane	NN
cord-048471-7jszm1nd	197	3	was	be	VBD
cord-048471-7jszm1nd	197	4	probed	probe	VBN
cord-048471-7jszm1nd	197	5	using	use	VBG
cord-048471-7jszm1nd	197	6	the	the	DT
cord-048471-7jszm1nd	197	7	anti	anti	JJ
cord-048471-7jszm1nd	197	8	-	-	JJ
cord-048471-7jszm1nd	197	9	V5	v5	JJ
cord-048471-7jszm1nd	197	10	monoclonal	monoclonal	JJ
cord-048471-7jszm1nd	197	11	antibody	antibody	NN
cord-048471-7jszm1nd	197	12	at	at	IN
cord-048471-7jszm1nd	197	13	the	the	DT
cord-048471-7jszm1nd	197	14	manufacturer	manufacturer	NN
cord-048471-7jszm1nd	197	15	's	's	POS
cord-048471-7jszm1nd	197	16	recommended	recommend	VBN
cord-048471-7jszm1nd	197	17	dilution	dilution	NN
cord-048471-7jszm1nd	197	18	,	,	,
cord-048471-7jszm1nd	197	19	followed	follow	VBN
cord-048471-7jszm1nd	197	20	by	by	IN
cord-048471-7jszm1nd	197	21	an	an	DT
cord-048471-7jszm1nd	197	22	anti	anti	JJ
cord-048471-7jszm1nd	197	23	-	-	JJ
cord-048471-7jszm1nd	197	24	mouse	mouse	JJ
cord-048471-7jszm1nd	197	25	HRP	HRP	NNP
cord-048471-7jszm1nd	197	26	-	-	HYPH
cord-048471-7jszm1nd	197	27	copnjugated	copnjugate	VBN
cord-048471-7jszm1nd	197	28	secondary	secondary	JJ
cord-048471-7jszm1nd	197	29	antibody	antibody	NN
cord-048471-7jszm1nd	197	30	(	(	-LRB-
cord-048471-7jszm1nd	197	31	Santa	Santa	NNP
cord-048471-7jszm1nd	197	32	Cruz	Cruz	NNP
cord-048471-7jszm1nd	197	33	)	)	-RRB-
cord-048471-7jszm1nd	197	34	at	at	IN
cord-048471-7jszm1nd	197	35	the	the	DT
cord-048471-7jszm1nd	197	36	recommended	recommend	VBN
cord-048471-7jszm1nd	197	37	diltution	diltution	NN
cord-048471-7jszm1nd	197	38	.	.	.
cord-048471-7jszm1nd	198	1	The	the	DT
cord-048471-7jszm1nd	198	2	ECL	ECL	NNP
cord-048471-7jszm1nd	198	3	Western	western	JJ
cord-048471-7jszm1nd	198	4	blotting	blotting	NN
cord-048471-7jszm1nd	198	5	reagents	reagent	NNS
cord-048471-7jszm1nd	198	6	kit	kit	NN
cord-048471-7jszm1nd	198	7	(	(	-LRB-
cord-048471-7jszm1nd	198	8	G.E.	G.E.	NNP
cord-048471-7jszm1nd	198	9	Healthcare	Healthcare	NNP
cord-048471-7jszm1nd	198	10	)	)	-RRB-
cord-048471-7jszm1nd	198	11	was	be	VBD
cord-048471-7jszm1nd	198	12	used	use	VBN
cord-048471-7jszm1nd	198	13	to	to	TO
cord-048471-7jszm1nd	198	14	detect	detect	VB
cord-048471-7jszm1nd	198	15	antibody	antibody	NN
cord-048471-7jszm1nd	198	16	bound	bind	VBN
cord-048471-7jszm1nd	198	17	protein	protein	NN
cord-048471-7jszm1nd	198	18	,	,	,
cord-048471-7jszm1nd	198	19	which	which	WDT
cord-048471-7jszm1nd	198	20	was	be	VBD
cord-048471-7jszm1nd	198	21	visualised	visualise	VBN
cord-048471-7jszm1nd	198	22	by	by	IN
cord-048471-7jszm1nd	198	23	exposure	exposure	NN
cord-048471-7jszm1nd	198	24	to	to	IN
cord-048471-7jszm1nd	198	25	BioMax	BioMax	NNP
cord-048471-7jszm1nd	198	26	Light	Light	NNP
cord-048471-7jszm1nd	198	27	film	film	NN
cord-048471-7jszm1nd	198	28	(	(	-LRB-
cord-048471-7jszm1nd	198	29	Kodak	Kodak	NNP
cord-048471-7jszm1nd	198	30	)	)	-RRB-
cord-048471-7jszm1nd	198	31	.	.	.
cord-048471-7jszm1nd	199	1	CRFK	CRFK	NNP
cord-048471-7jszm1nd	199	2	cells	cell	NNS
cord-048471-7jszm1nd	199	3	were	be	VBD
cord-048471-7jszm1nd	199	4	seeded	seed	VBN
cord-048471-7jszm1nd	199	5	into	into	IN
cord-048471-7jszm1nd	199	6	6	6	CD
cord-048471-7jszm1nd	199	7	well	well	NN
cord-048471-7jszm1nd	199	8	trays	tray	NNS
cord-048471-7jszm1nd	199	9	and	and	CC
cord-048471-7jszm1nd	199	10	transfected	transfecte	VBN
cord-048471-7jszm1nd	199	11	with	with	IN
cord-048471-7jszm1nd	199	12	capped	cap	VBN
cord-048471-7jszm1nd	199	13	JV	JV	NNP
cord-048471-7jszm1nd	199	14	V5	V5	NNP
cord-048471-7jszm1nd	199	15	RNA	RNA	NNP
cord-048471-7jszm1nd	199	16	as	as	IN
cord-048471-7jszm1nd	199	17	described	describe	VBN
cord-048471-7jszm1nd	199	18	.	.	.
cord-048471-7jszm1nd	200	1	Following	follow	VBG
cord-048471-7jszm1nd	200	2	a	a	DT
cord-048471-7jszm1nd	200	3	24	24	CD
cord-048471-7jszm1nd	200	4	hr	hr	NN
cord-048471-7jszm1nd	200	5	incubation	incubation	NN
cord-048471-7jszm1nd	200	6	cycloheximide	cycloheximide	NN
cord-048471-7jszm1nd	200	7	(	(	-LRB-
cord-048471-7jszm1nd	200	8	Sigma	Sigma	NNP
cord-048471-7jszm1nd	200	9	)	)	-RRB-
cord-048471-7jszm1nd	200	10	was	be	VBD
cord-048471-7jszm1nd	200	11	added	add	VBN
cord-048471-7jszm1nd	200	12	to	to	IN
cord-048471-7jszm1nd	200	13	the	the	DT
cord-048471-7jszm1nd	200	14	cells	cell	NNS
cord-048471-7jszm1nd	200	15	at	at	IN
cord-048471-7jszm1nd	200	16	a	a	DT
cord-048471-7jszm1nd	200	17	final	final	JJ
cord-048471-7jszm1nd	200	18	concentration	concentration	NN
cord-048471-7jszm1nd	200	19	of	of	IN
cord-048471-7jszm1nd	200	20	50	50	CD
cord-048471-7jszm1nd	200	21	mg	mg	NNP
cord-048471-7jszm1nd	200	22	/	/	SYM
cord-048471-7jszm1nd	200	23	ml	ml	NN
cord-048471-7jszm1nd	200	24	.	.	.
cord-048471-7jszm1nd	201	1	Cells	cell	NNS
cord-048471-7jszm1nd	201	2	were	be	VBD
cord-048471-7jszm1nd	201	3	harvested	harvest	VBN
cord-048471-7jszm1nd	201	4	at	at	IN
cord-048471-7jszm1nd	201	5	indicated	indicate	VBN
cord-048471-7jszm1nd	201	6	times	time	NNS
cord-048471-7jszm1nd	201	7	for	for	IN
cord-048471-7jszm1nd	201	8	the	the	DT
cord-048471-7jszm1nd	201	9	preparation	preparation	NN
cord-048471-7jszm1nd	201	10	of	of	IN
cord-048471-7jszm1nd	201	11	lysates	lysate	NNS
cord-048471-7jszm1nd	201	12	for	for	IN
cord-048471-7jszm1nd	201	13	V5	v5	JJ
cord-048471-7jszm1nd	201	14	Western	western	JJ
cord-048471-7jszm1nd	201	15	analysis	analysis	NN
cord-048471-7jszm1nd	201	16	as	as	IN
cord-048471-7jszm1nd	201	17	described	describe	VBN
cord-048471-7jszm1nd	201	18	above	above	RB
cord-048471-7jszm1nd	201	19	.	.	.
cord-048471-7jszm1nd	202	1	Bradford	Bradford	NNP
cord-048471-7jszm1nd	202	2	reagent	reagent	NN
cord-048471-7jszm1nd	202	3	(	(	-LRB-
cord-048471-7jszm1nd	202	4	Sigma	Sigma	NNP
cord-048471-7jszm1nd	202	5	)	)	-RRB-
cord-048471-7jszm1nd	202	6	was	be	VBD
cord-048471-7jszm1nd	202	7	used	use	VBN
cord-048471-7jszm1nd	202	8	to	to	TO
cord-048471-7jszm1nd	202	9	ensure	ensure	VB
cord-048471-7jszm1nd	202	10	equal	equal	JJ
cord-048471-7jszm1nd	202	11	loading	loading	NN
cord-048471-7jszm1nd	202	12	of	of	IN
cord-048471-7jszm1nd	202	13	lysates	lysate	NNS
cord-048471-7jszm1nd	202	14	according	accord	VBG
cord-048471-7jszm1nd	202	15	to	to	IN
cord-048471-7jszm1nd	202	16	the	the	DT
cord-048471-7jszm1nd	202	17	manufacturer	manufacturer	NN
cord-048471-7jszm1nd	202	18	's	's	POS
cord-048471-7jszm1nd	202	19	recommendations	recommendation	NNS
cord-048471-7jszm1nd	202	20	.	.	.
cord-048471-7jszm1nd	203	1	Studies	study	NNS
cord-048471-7jszm1nd	203	2	into	into	IN
cord-048471-7jszm1nd	203	3	diarrhoea	diarrhoea	NN
cord-048471-7jszm1nd	203	4	of	of	IN
cord-048471-7jszm1nd	203	5	young	young	JJ
cord-048471-7jszm1nd	203	6	calves	calf	NNS
cord-048471-7jszm1nd	203	7	.	.	.
cord-048471-7jszm1nd	204	1	Sixth	sixth	JJ
cord-048471-7jszm1nd	204	2	communication	communication	NN
cord-048471-7jszm1nd	204	3	:	:	:
cord-048471-7jszm1nd	204	4	Detection	detection	NN
cord-048471-7jszm1nd	204	5	and	and	CC
cord-048471-7jszm1nd	204	6	determination	determination	NN
cord-048471-7jszm1nd	204	7	of	of	IN
cord-048471-7jszm1nd	204	8	pathogenicity	pathogenicity	NN
cord-048471-7jszm1nd	204	9	of	of	IN
cord-048471-7jszm1nd	204	10	a	a	DT
cord-048471-7jszm1nd	204	11	bovine	bovine	JJ
cord-048471-7jszm1nd	204	12	Corona	Corona	NNP
cord-048471-7jszm1nd	204	13	virus	virus	NN
cord-048471-7jszm1nd	204	14	and	and	CC
cord-048471-7jszm1nd	204	15	an	an	DT
cord-048471-7jszm1nd	204	16	undefined	undefined	JJ
cord-048471-7jszm1nd	204	17	icosahedric	icosahedric	JJ
cord-048471-7jszm1nd	204	18	virus	virus	NN
cord-048471-7jszm1nd	204	19	.	.	.
cord-048471-7jszm1nd	205	1	Archives	archive	NNS
cord-048471-7jszm1nd	205	2	of	of	IN
cord-048471-7jszm1nd	205	3	experimental	experimental	JJ
cord-048471-7jszm1nd	205	4	Veterinary	veterinary	JJ
cord-048471-7jszm1nd	205	5	medicine	medicine	NN
cord-048471-7jszm1nd	205	6	Studies	Studies	NNPS
cord-048471-7jszm1nd	205	7	into	into	IN
cord-048471-7jszm1nd	205	8	diarrhoea	diarrhoea	NN
cord-048471-7jszm1nd	205	9	of	of	IN
cord-048471-7jszm1nd	205	10	young	young	JJ
cord-048471-7jszm1nd	205	11	calves	calf	NNS
cord-048471-7jszm1nd	205	12	-	-	:
cord-048471-7jszm1nd	205	13	Seventh	Seventh	NNP
cord-048471-7jszm1nd	205	14	Communication	Communication	NNP
cord-048471-7jszm1nd	205	15	:	:	:
cord-048471-7jszm1nd	205	16	'	'	''
cord-048471-7jszm1nd	205	17	'	'	``
cord-048471-7jszm1nd	205	18	Zackenvirus	Zackenvirus	NNP
cord-048471-7jszm1nd	205	19	'	'	''
cord-048471-7jszm1nd	205	20	'	'	''
cord-048471-7jszm1nd	206	1	(	(	-LRB-
cord-048471-7jszm1nd	206	2	Jena	Jena	NNP
cord-048471-7jszm1nd	206	3	-	-	HYPH
cord-048471-7jszm1nd	206	4	Agens	Agens	NNP
cord-048471-7jszm1nd	206	5	117/80)-A	117/80)-a	CD
cord-048471-7jszm1nd	206	6	new	new	JJ
cord-048471-7jszm1nd	206	7	diarrhoea	diarrhoea	NN
cord-048471-7jszm1nd	206	8	pathogen	pathogen	NN
cord-048471-7jszm1nd	206	9	to	to	IN
cord-048471-7jszm1nd	206	10	calf	calf	NN
cord-048471-7jszm1nd	206	11	.	.	.
cord-048471-7jszm1nd	207	1	Archives	archive	NNS
cord-048471-7jszm1nd	207	2	of	of	IN
cord-048471-7jszm1nd	207	3	experimental	experimental	JJ
cord-048471-7jszm1nd	207	4	Veterinary	veterinary	JJ
cord-048471-7jszm1nd	207	5	medicine	medicine	NN
cord-048471-7jszm1nd	207	6	Complete	complete	VB
cord-048471-7jszm1nd	207	7	genomic	genomic	JJ
cord-048471-7jszm1nd	207	8	characterization	characterization	NN
cord-048471-7jszm1nd	207	9	and	and	CC
cord-048471-7jszm1nd	207	10	antigenic	antigenic	JJ
cord-048471-7jszm1nd	207	11	relatedness	relatedness	NN
cord-048471-7jszm1nd	207	12	of	of	IN
cord-048471-7jszm1nd	207	13	genogroup	genogroup	NNP
cord-048471-7jszm1nd	207	14	III	III	NNP
cord-048471-7jszm1nd	207	15	,	,	,
cord-048471-7jszm1nd	207	16	genotype	genotype	NN
cord-048471-7jszm1nd	207	17	2	2	CD
cord-048471-7jszm1nd	207	18	bovine	bovine	JJ
cord-048471-7jszm1nd	207	19	noroviruses	noroviruse	NNS
cord-048471-7jszm1nd	208	1	Genotype	genotype	NN
cord-048471-7jszm1nd	208	2	1	1	CD
cord-048471-7jszm1nd	208	3	and	and	CC
cord-048471-7jszm1nd	208	4	Genotype	Genotype	NNP
cord-048471-7jszm1nd	208	5	2	2	CD
cord-048471-7jszm1nd	208	6	Bovine	Bovine	NNP
cord-048471-7jszm1nd	208	7	Noroviruses	Noroviruses	NNPS
cord-048471-7jszm1nd	208	8	Are	be	VBP
cord-048471-7jszm1nd	208	9	Antigenically	Antigenically	NNP
cord-048471-7jszm1nd	208	10	Distinct	distinct	JJ
cord-048471-7jszm1nd	208	11	but	but	CC
cord-048471-7jszm1nd	208	12	Share	share	VBP
cord-048471-7jszm1nd	208	13	a	a	DT
cord-048471-7jszm1nd	208	14	Cross	Cross	NNP
cord-048471-7jszm1nd	208	15	-	-	HYPH
cord-048471-7jszm1nd	208	16	Reactive	reactive	JJ
cord-048471-7jszm1nd	208	17	Epitope	Epitope	NNP
cord-048471-7jszm1nd	208	18	with	with	IN
cord-048471-7jszm1nd	208	19	Human	Human	NNP
cord-048471-7jszm1nd	208	20	Noroviruses	Noroviruses	NNP
cord-048471-7jszm1nd	208	21	Characterization	Characterization	NNP
cord-048471-7jszm1nd	208	22	of	of	IN
cord-048471-7jszm1nd	208	23	a	a	DT
cord-048471-7jszm1nd	208	24	calici	calici	VBN
cord-048471-7jszm1nd	208	25	-	-	HYPH
cord-048471-7jszm1nd	208	26	like	like	JJ
cord-048471-7jszm1nd	208	27	virus	virus	NN
cord-048471-7jszm1nd	208	28	(	(	-LRB-
cord-048471-7jszm1nd	208	29	Newbury	Newbury	NNP
cord-048471-7jszm1nd	208	30	agent	agent	NN
cord-048471-7jszm1nd	208	31	)	)	-RRB-
cord-048471-7jszm1nd	208	32	found	find	VBD
cord-048471-7jszm1nd	208	33	in	in	IN
cord-048471-7jszm1nd	208	34	association	association	NN
cord-048471-7jszm1nd	208	35	with	with	IN
cord-048471-7jszm1nd	208	36	astrovirus	astrovirus	NNP
cord-048471-7jszm1nd	208	37	in	in	IN
cord-048471-7jszm1nd	208	38	bovine	bovine	JJ
cord-048471-7jszm1nd	208	39	diarrhea	diarrhea	NN
cord-048471-7jszm1nd	209	1	The	the	DT
cord-048471-7jszm1nd	209	2	Seroepidemiology	Seroepidemiology	NNP
cord-048471-7jszm1nd	209	3	of	of	IN
cord-048471-7jszm1nd	209	4	genogroup	genogroup	NN
cord-048471-7jszm1nd	209	5	1	1	CD
cord-048471-7jszm1nd	209	6	and	and	CC
cord-048471-7jszm1nd	209	7	genogroup	genogroup	NNP
cord-048471-7jszm1nd	209	8	2	2	CD
cord-048471-7jszm1nd	209	9	Norwalk	Norwalk	NNP
cord-048471-7jszm1nd	209	10	-	-	HYPH
cord-048471-7jszm1nd	209	11	like	like	JJ
cord-048471-7jszm1nd	209	12	viruses	virus	NNS
cord-048471-7jszm1nd	209	13	in	in	IN
cord-048471-7jszm1nd	209	14	Italy	Italy	NNP
cord-048471-7jszm1nd	209	15	Molecular	Molecular	NNP
cord-048471-7jszm1nd	209	16	characterization	characterization	NN
cord-048471-7jszm1nd	209	17	of	of	IN
cord-048471-7jszm1nd	209	18	a	a	DT
cord-048471-7jszm1nd	209	19	bovine	bovine	JJ
cord-048471-7jszm1nd	209	20	enteric	enteric	JJ
cord-048471-7jszm1nd	209	21	calicivirus	calicivirus	NN
cord-048471-7jszm1nd	209	22	:	:	:
cord-048471-7jszm1nd	209	23	relationship	relationship	NN
cord-048471-7jszm1nd	209	24	to	to	IN
cord-048471-7jszm1nd	209	25	the	the	DT
cord-048471-7jszm1nd	209	26	Norwalk	Norwalk	NNP
cord-048471-7jszm1nd	209	27	-	-	HYPH
cord-048471-7jszm1nd	209	28	like	like	JJ
cord-048471-7jszm1nd	209	29	viruses	virus	NNS
cord-048471-7jszm1nd	209	30	Two	two	CD
cord-048471-7jszm1nd	209	31	nonoverlapping	nonoverlappe	VBG
cord-048471-7jszm1nd	209	32	domains	domain	NNS
cord-048471-7jszm1nd	209	33	on	on	IN
cord-048471-7jszm1nd	209	34	the	the	DT
cord-048471-7jszm1nd	209	35	Norwalk	Norwalk	NNP
cord-048471-7jszm1nd	209	36	virus	virus	NN
cord-048471-7jszm1nd	209	37	open	open	JJ
cord-048471-7jszm1nd	209	38	reading	reading	NN
cord-048471-7jszm1nd	209	39	frame	frame	NN
cord-048471-7jszm1nd	209	40	3	3	CD
cord-048471-7jszm1nd	210	1	(	(	-LRB-
cord-048471-7jszm1nd	210	2	ORF3	ORF3	NNP
cord-048471-7jszm1nd	210	3	)	)	-RRB-
cord-048471-7jszm1nd	210	4	protein	protein	NN
cord-048471-7jszm1nd	210	5	are	be	VBP
cord-048471-7jszm1nd	210	6	involved	involve	VBN
cord-048471-7jszm1nd	210	7	in	in	IN
cord-048471-7jszm1nd	210	8	the	the	DT
cord-048471-7jszm1nd	210	9	formation	formation	NN
cord-048471-7jszm1nd	210	10	of	of	IN
cord-048471-7jszm1nd	210	11	the	the	DT
cord-048471-7jszm1nd	210	12	phosphorylated	phosphorylated	JJ
cord-048471-7jszm1nd	210	13	35	35	CD
cord-048471-7jszm1nd	210	14	K	K	NNP
cord-048471-7jszm1nd	210	15	protein	protein	NN
cord-048471-7jszm1nd	210	16	and	and	CC
cord-048471-7jszm1nd	210	17	in	in	IN
cord-048471-7jszm1nd	210	18	ORF3-capsid	orf3-capsid	JJ
cord-048471-7jszm1nd	210	19	protein	protein	NN
cord-048471-7jszm1nd	210	20	interactions	interaction	NNS
cord-048471-7jszm1nd	210	21	Processing	process	VBG
cord-048471-7jszm1nd	210	22	map	map	NN
cord-048471-7jszm1nd	210	23	and	and	CC
cord-048471-7jszm1nd	210	24	essential	essential	JJ
cord-048471-7jszm1nd	210	25	cleavage	cleavage	NN
cord-048471-7jszm1nd	210	26	sites	site	NNS
cord-048471-7jszm1nd	210	27	of	of	IN
cord-048471-7jszm1nd	210	28	the	the	DT
cord-048471-7jszm1nd	210	29	nonstructural	nonstructural	JJ
cord-048471-7jszm1nd	210	30	polyprotein	polyprotein	NN
cord-048471-7jszm1nd	210	31	encoded	encode	VBN
cord-048471-7jszm1nd	210	32	by	by	IN
cord-048471-7jszm1nd	210	33	ORF1	ORF1	NNP
cord-048471-7jszm1nd	210	34	of	of	IN
cord-048471-7jszm1nd	210	35	the	the	DT
cord-048471-7jszm1nd	210	36	feline	feline	JJ
cord-048471-7jszm1nd	210	37	calicivirus	calicivirus	NNP
cord-048471-7jszm1nd	210	38	genome	genome	NN
cord-048471-7jszm1nd	210	39	Rabbit	Rabbit	NNP
cord-048471-7jszm1nd	210	40	Hemorrhagic	Hemorrhagic	NNP
cord-048471-7jszm1nd	210	41	Disease	Disease	NNP
cord-048471-7jszm1nd	210	42	Virus	virus	NN
cord-048471-7jszm1nd	210	43	:	:	:
cord-048471-7jszm1nd	210	44	Genome	Genome	NNP
cord-048471-7jszm1nd	210	45	Organisation	Organisation	NNP
cord-048471-7jszm1nd	210	46	and	and	CC
cord-048471-7jszm1nd	210	47	Polyprotein	Polyprotein	NNP
cord-048471-7jszm1nd	210	48	Processing	Processing	NNP
cord-048471-7jszm1nd	210	49	of	of	IN
cord-048471-7jszm1nd	210	50	a	a	DT
cord-048471-7jszm1nd	210	51	Calicivirus	Calicivirus	NNP
cord-048471-7jszm1nd	210	52	Studied	study	VBN
cord-048471-7jszm1nd	210	53	after	after	IN
cord-048471-7jszm1nd	210	54	Transient	transient	JJ
cord-048471-7jszm1nd	210	55	Expression	expression	NN
cord-048471-7jszm1nd	210	56	of	of	IN
cord-048471-7jszm1nd	210	57	cDNA	cdna	NN
cord-048471-7jszm1nd	210	58	Constructs	Constructs	NNPS
cord-048471-7jszm1nd	210	59	Organisation	Organisation	NNP
cord-048471-7jszm1nd	210	60	and	and	CC
cord-048471-7jszm1nd	210	61	Expression	Expression	NNP
cord-048471-7jszm1nd	210	62	of	of	IN
cord-048471-7jszm1nd	210	63	Calicivirus	Calicivirus	NNP
cord-048471-7jszm1nd	210	64	Genes	Genes	NNPS
cord-048471-7jszm1nd	210	65	Cleavage	Cleavage	NNP
cord-048471-7jszm1nd	210	66	map	map	NN
cord-048471-7jszm1nd	210	67	and	and	CC
cord-048471-7jszm1nd	210	68	proteolytic	proteolytic	JJ
cord-048471-7jszm1nd	210	69	processing	processing	NN
cord-048471-7jszm1nd	210	70	of	of	IN
cord-048471-7jszm1nd	210	71	the	the	DT
cord-048471-7jszm1nd	210	72	murine	murine	JJ
cord-048471-7jszm1nd	210	73	norovirus	norovirus	NN
cord-048471-7jszm1nd	210	74	nonstructural	nonstructural	JJ
cord-048471-7jszm1nd	210	75	polyprotein	polyprotein	NN
cord-048471-7jszm1nd	210	76	in	in	IN
cord-048471-7jszm1nd	210	77	infected	infected	JJ
cord-048471-7jszm1nd	210	78	cells	cell	NNS
cord-048471-7jszm1nd	210	79	Norovirus	norovirus	IN
cord-048471-7jszm1nd	210	80	protein	protein	NN
cord-048471-7jszm1nd	210	81	structure	structure	NN
cord-048471-7jszm1nd	210	82	and	and	CC
cord-048471-7jszm1nd	210	83	function	function	VB
cord-048471-7jszm1nd	210	84	Open	open	JJ
cord-048471-7jszm1nd	210	85	reading	reading	NN
cord-048471-7jszm1nd	210	86	frame	frame	NN
cord-048471-7jszm1nd	210	87	1	1	CD
cord-048471-7jszm1nd	210	88	of	of	IN
cord-048471-7jszm1nd	210	89	the	the	DT
cord-048471-7jszm1nd	210	90	Norwalklike	Norwalklike	NNP
cord-048471-7jszm1nd	210	91	virus	virus	NN
cord-048471-7jszm1nd	210	92	Camberwell	Camberwell	NNP
cord-048471-7jszm1nd	210	93	:	:	:
cord-048471-7jszm1nd	210	94	Completion	completion	NN
cord-048471-7jszm1nd	210	95	of	of	IN
cord-048471-7jszm1nd	210	96	sequence	sequence	NN
cord-048471-7jszm1nd	210	97	and	and	CC
cord-048471-7jszm1nd	210	98	expression	expression	NN
cord-048471-7jszm1nd	210	99	in	in	IN
cord-048471-7jszm1nd	210	100	mammalian	mammalian	JJ
cord-048471-7jszm1nd	210	101	cells	cell	NNS
cord-048471-7jszm1nd	211	1	Norwalk	Norwalk	NNP
cord-048471-7jszm1nd	211	2	virus	virus	NN
cord-048471-7jszm1nd	211	3	N	n	CD
cord-048471-7jszm1nd	211	4	-	-	HYPH
cord-048471-7jszm1nd	211	5	terminal	terminal	JJ
cord-048471-7jszm1nd	211	6	nonstructural	nonstructural	JJ
cord-048471-7jszm1nd	211	7	protein	protein	NN
cord-048471-7jszm1nd	211	8	is	be	VBZ
cord-048471-7jszm1nd	211	9	associated	associate	VBN
cord-048471-7jszm1nd	211	10	with	with	IN
cord-048471-7jszm1nd	211	11	disassembly	disassembly	NN
cord-048471-7jszm1nd	211	12	of	of	IN
cord-048471-7jszm1nd	211	13	the	the	DT
cord-048471-7jszm1nd	211	14	Golgi	Golgi	NNP
cord-048471-7jszm1nd	211	15	complex	complex	NN
cord-048471-7jszm1nd	211	16	in	in	IN
cord-048471-7jszm1nd	211	17	transfected	transfecte	VBN
cord-048471-7jszm1nd	211	18	cells	cell	NNS
cord-048471-7jszm1nd	211	19	Norwalk	Norwalk	NNP
cord-048471-7jszm1nd	211	20	virus	virus	NN
cord-048471-7jszm1nd	211	21	nonstructural	nonstructural	JJ
cord-048471-7jszm1nd	211	22	protein	protein	NNP
cord-048471-7jszm1nd	211	23	p48	p48	NNP
cord-048471-7jszm1nd	211	24	forms	form	VBZ
cord-048471-7jszm1nd	211	25	a	a	DT
cord-048471-7jszm1nd	211	26	complex	complex	NN
cord-048471-7jszm1nd	211	27	with	with	IN
cord-048471-7jszm1nd	211	28	the	the	DT
cord-048471-7jszm1nd	211	29	SNARE	snare	NN
cord-048471-7jszm1nd	211	30	regulator	regulator	NN
cord-048471-7jszm1nd	211	31	VAP	VAP	NNP
cord-048471-7jszm1nd	211	32	-	-	HYPH
cord-048471-7jszm1nd	211	33	A	A	NNP
cord-048471-7jszm1nd	211	34	and	and	CC
cord-048471-7jszm1nd	211	35	prevents	prevent	VBZ
cord-048471-7jszm1nd	211	36	cell	cell	NN
cord-048471-7jszm1nd	211	37	surface	surface	NN
cord-048471-7jszm1nd	211	38	expression	expression	NN
cord-048471-7jszm1nd	211	39	of	of	IN
cord-048471-7jszm1nd	211	40	vesicular	vesicular	JJ
cord-048471-7jszm1nd	211	41	stomatitis	stomatitis	NN
cord-048471-7jszm1nd	211	42	virus	virus	NN
cord-048471-7jszm1nd	211	43	G	g	NN
cord-048471-7jszm1nd	211	44	protein	protein	NN
cord-048471-7jszm1nd	211	45	Activity	activity	NN
cord-048471-7jszm1nd	211	46	of	of	IN
cord-048471-7jszm1nd	211	47	the	the	DT
cord-048471-7jszm1nd	211	48	Norovirus	Norovirus	NNP
cord-048471-7jszm1nd	211	49	Camberwell	Camberwell	NNP
cord-048471-7jszm1nd	211	50	Proteinase	Proteinase	NNP
cord-048471-7jszm1nd	211	51	and	and	CC
cord-048471-7jszm1nd	211	52	Cleavage	Cleavage	NNP
cord-048471-7jszm1nd	211	53	of	of	IN
cord-048471-7jszm1nd	211	54	the	the	DT
cord-048471-7jszm1nd	211	55	N	N	NNP
cord-048471-7jszm1nd	211	56	-	-	HYPH
cord-048471-7jszm1nd	211	57	Terminal	Terminal	NNP
cord-048471-7jszm1nd	211	58	Protein	Protein	NNP
cord-048471-7jszm1nd	211	59	Encoded	encode	VBN
cord-048471-7jszm1nd	211	60	by	by	IN
cord-048471-7jszm1nd	211	61	ORF1	ORF1	NNP
cord-048471-7jszm1nd	211	62	Sequence	Sequence	NNP
cord-048471-7jszm1nd	211	63	and	and	CC
cord-048471-7jszm1nd	211	64	genomic	genomic	JJ
cord-048471-7jszm1nd	211	65	organization	organization	NN
cord-048471-7jszm1nd	211	66	of	of	IN
cord-048471-7jszm1nd	211	67	Norwalk	Norwalk	NNP
cord-048471-7jszm1nd	211	68	virus	virus	NN
cord-048471-7jszm1nd	211	69	La	La	NNP
cord-048471-7jszm1nd	211	70	,	,	,
cord-048471-7jszm1nd	211	71	PTB	PTB	NNP
cord-048471-7jszm1nd	211	72	,	,	,
cord-048471-7jszm1nd	211	73	and	and	CC
cord-048471-7jszm1nd	211	74	PAB	PAB	NNP
cord-048471-7jszm1nd	211	75	proteins	protein	NNS
cord-048471-7jszm1nd	211	76	bind	bind	VBP
cord-048471-7jszm1nd	211	77	to	to	IN
cord-048471-7jszm1nd	211	78	the	the	DT
cord-048471-7jszm1nd	211	79	39	39	CD
cord-048471-7jszm1nd	211	80	untranslated	untranslated	JJ
cord-048471-7jszm1nd	211	81	region	region	NN
cord-048471-7jszm1nd	211	82	of	of	IN
cord-048471-7jszm1nd	211	83	Norwalk	Norwalk	NNP
cord-048471-7jszm1nd	211	84	virus	virus	NN
cord-048471-7jszm1nd	211	85	genomic	genomic	JJ
cord-048471-7jszm1nd	211	86	RNA	RNA	NNP
cord-048471-7jszm1nd	211	87	Requirement	Requirement	NNP
cord-048471-7jszm1nd	211	88	of	of	IN
cord-048471-7jszm1nd	211	89	Poly(rC	Poly(rC	NNP
cord-048471-7jszm1nd	211	90	)	)	-RRB-
cord-048471-7jszm1nd	212	1	binding	bind	VBG
cord-048471-7jszm1nd	212	2	protein	protein	NN
cord-048471-7jszm1nd	212	3	2	2	CD
cord-048471-7jszm1nd	212	4	for	for	IN
cord-048471-7jszm1nd	212	5	translation	translation	NN
cord-048471-7jszm1nd	212	6	of	of	IN
cord-048471-7jszm1nd	212	7	poliovirus	poliovirus	NN
cord-048471-7jszm1nd	212	8	RNA	RNA	NNP
cord-048471-7jszm1nd	212	9	Stem	Stem	NNP
cord-048471-7jszm1nd	212	10	-	-	HYPH
cord-048471-7jszm1nd	212	11	Loop	Loop	NNP
cord-048471-7jszm1nd	212	12	Structure	Structure	NNP
cord-048471-7jszm1nd	212	13	Synergy	Synergy	NNP
cord-048471-7jszm1nd	212	14	in	in	IN
cord-048471-7jszm1nd	212	15	Binding	bind	VBG
cord-048471-7jszm1nd	212	16	Cellular	Cellular	NNP
cord-048471-7jszm1nd	212	17	Proteins	Proteins	NNPS
cord-048471-7jszm1nd	212	18	to	to	IN
cord-048471-7jszm1nd	212	19	the	the	DT
cord-048471-7jszm1nd	212	20	59	59	CD
cord-048471-7jszm1nd	212	21	Noncoding	Noncoding	NNP
cord-048471-7jszm1nd	212	22	Region	Region	NNP
cord-048471-7jszm1nd	212	23	of	of	IN
cord-048471-7jszm1nd	212	24	Poliovirus	Poliovirus	NNP
cord-048471-7jszm1nd	212	25	Rna	Rna	NNP
cord-048471-7jszm1nd	212	26	Direct	direct	JJ
cord-048471-7jszm1nd	212	27	evidence	evidence	NN
cord-048471-7jszm1nd	212	28	that	that	IN
cord-048471-7jszm1nd	212	29	polypyrimidine	polypyrimidine	NNP
cord-048471-7jszm1nd	212	30	tract	tract	NN
cord-048471-7jszm1nd	212	31	binding	bind	VBG
cord-048471-7jszm1nd	212	32	protein	protein	NN
cord-048471-7jszm1nd	212	33	(	(	-LRB-
cord-048471-7jszm1nd	212	34	PTB	PTB	NNP
cord-048471-7jszm1nd	212	35	)	)	-RRB-
cord-048471-7jszm1nd	212	36	is	be	VBZ
cord-048471-7jszm1nd	212	37	essential	essential	JJ
cord-048471-7jszm1nd	212	38	for	for	IN
cord-048471-7jszm1nd	212	39	internal	internal	JJ
cord-048471-7jszm1nd	212	40	initiation	initiation	NN
cord-048471-7jszm1nd	212	41	of	of	IN
cord-048471-7jszm1nd	212	42	translation	translation	NN
cord-048471-7jszm1nd	212	43	of	of	IN
cord-048471-7jszm1nd	212	44	encephalomyocarditis	encephalomyocarditis	NNP
cord-048471-7jszm1nd	212	45	virus	virus	NN
cord-048471-7jszm1nd	212	46	RNA	RNA	NNP
cord-048471-7jszm1nd	212	47	Sonenberg	Sonenberg	NNP
cord-048471-7jszm1nd	212	48	N	N	NNP
cord-048471-7jszm1nd	212	49	(	(	-LRB-
cord-048471-7jszm1nd	212	50	1993	1993	CD
cord-048471-7jszm1nd	212	51	)	)	-RRB-
cord-048471-7jszm1nd	213	1	La	La	NNP
cord-048471-7jszm1nd	213	2	autoantigen	autoantigen	NNP
cord-048471-7jszm1nd	213	3	enhances	enhance	VBZ
cord-048471-7jszm1nd	213	4	and	and	CC
cord-048471-7jszm1nd	213	5	corrects	correct	VBZ
cord-048471-7jszm1nd	213	6	aberrant	aberrant	JJ
cord-048471-7jszm1nd	213	7	translation	translation	NN
cord-048471-7jszm1nd	213	8	of	of	IN
cord-048471-7jszm1nd	213	9	poliovirus	poliovirus	NN
cord-048471-7jszm1nd	213	10	RNA	RNA	NNP
cord-048471-7jszm1nd	213	11	in	in	IN
cord-048471-7jszm1nd	213	12	reticulocyte	reticulocyte	NN
cord-048471-7jszm1nd	213	13	lysate	lysate	NN
cord-048471-7jszm1nd	213	14	Interaction	Interaction	NNP
cord-048471-7jszm1nd	213	15	of	of	IN
cord-048471-7jszm1nd	213	16	cellular	cellular	JJ
cord-048471-7jszm1nd	213	17	proteins	protein	NNS
cord-048471-7jszm1nd	213	18	with	with	IN
cord-048471-7jszm1nd	213	19	the	the	DT
cord-048471-7jszm1nd	213	20	59	59	CD
cord-048471-7jszm1nd	213	21	end	end	NN
cord-048471-7jszm1nd	213	22	of	of	IN
cord-048471-7jszm1nd	213	23	Norwalk	Norwalk	NNP
cord-048471-7jszm1nd	213	24	virus	virus	NN
cord-048471-7jszm1nd	213	25	genomic	genomic	JJ
cord-048471-7jszm1nd	213	26	RNA	RNA	NNP
cord-048471-7jszm1nd	214	1	The	the	DT
cord-048471-7jszm1nd	214	2	genomelinked	genomelinked	JJ
cord-048471-7jszm1nd	214	3	protein	protein	NN
cord-048471-7jszm1nd	214	4	VPg	VPg	NNS
cord-048471-7jszm1nd	214	5	of	of	IN
cord-048471-7jszm1nd	214	6	the	the	DT
cord-048471-7jszm1nd	214	7	Norwalk	Norwalk	NNP
cord-048471-7jszm1nd	214	8	virus	virus	NN
cord-048471-7jszm1nd	214	9	binds	bind	VBZ
cord-048471-7jszm1nd	214	10	eIF3	eIF3	NNP
cord-048471-7jszm1nd	214	11	,	,	,
cord-048471-7jszm1nd	214	12	suggesting	suggest	VBG
cord-048471-7jszm1nd	214	13	its	-PRON-	PRP$
cord-048471-7jszm1nd	214	14	role	role	NN
cord-048471-7jszm1nd	214	15	in	in	IN
cord-048471-7jszm1nd	214	16	translation	translation	NN
cord-048471-7jszm1nd	214	17	initiation	initiation	NN
cord-048471-7jszm1nd	214	18	complex	complex	JJ
cord-048471-7jszm1nd	214	19	recruitment	recruitment	NN
cord-048471-7jszm1nd	214	20	VPg	VPg	NNS
cord-048471-7jszm1nd	214	21	of	of	IN
cord-048471-7jszm1nd	214	22	murine	murine	JJ
cord-048471-7jszm1nd	214	23	norovirus	norovirus	NN
cord-048471-7jszm1nd	214	24	binds	bind	VBZ
cord-048471-7jszm1nd	214	25	translation	translation	NN
cord-048471-7jszm1nd	214	26	initiation	initiation	NN
cord-048471-7jszm1nd	214	27	factors	factor	NNS
cord-048471-7jszm1nd	214	28	in	in	IN
cord-048471-7jszm1nd	214	29	infected	infected	JJ
cord-048471-7jszm1nd	214	30	cells	cell	NNS
cord-048471-7jszm1nd	214	31	New	new	JJ
cord-048471-7jszm1nd	214	32	Ways	way	NNS
cord-048471-7jszm1nd	214	33	of	of	IN
cord-048471-7jszm1nd	214	34	Initiating	initiate	VBG
cord-048471-7jszm1nd	214	35	Translation	translation	NN
cord-048471-7jszm1nd	214	36	in	in	IN
cord-048471-7jszm1nd	214	37	Eukaryotes	eukaryote	NNS
cord-048471-7jszm1nd	214	38	?	?	.
cord-048471-7jszm1nd	215	1	Polyprotein	polyprotein	NN
cord-048471-7jszm1nd	215	2	processing	processing	NN
cord-048471-7jszm1nd	215	3	in	in	IN
cord-048471-7jszm1nd	215	4	Southampton	Southampton	NNP
cord-048471-7jszm1nd	215	5	virus	virus	NN
cord-048471-7jszm1nd	215	6	:	:	:
cord-048471-7jszm1nd	215	7	cleavage	cleavage	NN
cord-048471-7jszm1nd	215	8	in	in	IN
cord-048471-7jszm1nd	215	9	trans	trans	NNP
cord-048471-7jszm1nd	215	10	by	by	IN
cord-048471-7jszm1nd	215	11	the	the	DT
cord-048471-7jszm1nd	215	12	3C	3c	NN
cord-048471-7jszm1nd	215	13	-	-	HYPH
cord-048471-7jszm1nd	215	14	like	like	JJ
cord-048471-7jszm1nd	215	15	protease	protease	NN
cord-048471-7jszm1nd	215	16	Identification	identification	NN
cord-048471-7jszm1nd	215	17	of	of	IN
cord-048471-7jszm1nd	215	18	An	an	DT
cord-048471-7jszm1nd	215	19	Epitope	Epitope	NNP
cord-048471-7jszm1nd	215	20	on	on	IN
cord-048471-7jszm1nd	215	21	the	the	DT
cord-048471-7jszm1nd	215	22	P	p	NN
cord-048471-7jszm1nd	215	23	-	-	HYPH
cord-048471-7jszm1nd	215	24	Proteins	protein	NNS
cord-048471-7jszm1nd	215	25	and	and	CC
cord-048471-7jszm1nd	215	26	V	v	NN
cord-048471-7jszm1nd	215	27	-	-	HYPH
cord-048471-7jszm1nd	215	28	Proteins	protein	NNS
cord-048471-7jszm1nd	215	29	of	of	IN
cord-048471-7jszm1nd	215	30	Simian	Simian	NNP
cord-048471-7jszm1nd	215	31	-	-	HYPH
cord-048471-7jszm1nd	215	32	Virus	virus	NN
cord-048471-7jszm1nd	215	33	5	5	CD
cord-048471-7jszm1nd	215	34	That	that	WDT
cord-048471-7jszm1nd	215	35	Distinguishes	distinguish	VBZ
cord-048471-7jszm1nd	215	36	Between	between	IN
cord-048471-7jszm1nd	215	37	2	2	CD
cord-048471-7jszm1nd	215	38	Isolates	Isolates	NNPS
cord-048471-7jszm1nd	215	39	with	with	IN
cord-048471-7jszm1nd	215	40	Different	Different	NNP
cord-048471-7jszm1nd	215	41	Biological	Biological	NNP
cord-048471-7jszm1nd	215	42	Characteristics	Characteristics	NNPS
cord-048471-7jszm1nd	215	43	Electron	Electron	NNP
cord-048471-7jszm1nd	215	44	-	-	HYPH
cord-048471-7jszm1nd	215	45	Microscopic	Microscopic	NNP
cord-048471-7jszm1nd	215	46	Observation	Observation	NNP
cord-048471-7jszm1nd	215	47	of	of	IN
cord-048471-7jszm1nd	215	48	Feline	feline	JJ
cord-048471-7jszm1nd	215	49	Kidney	Kidney	NNP
cord-048471-7jszm1nd	215	50	-	-	HYPH
cord-048471-7jszm1nd	215	51	Cells	cell	NNS
cord-048471-7jszm1nd	215	52	Infected	infect	VBN
cord-048471-7jszm1nd	215	53	with	with	IN
cord-048471-7jszm1nd	215	54	A	a	DT
cord-048471-7jszm1nd	215	55	Feline	feline	JJ
cord-048471-7jszm1nd	215	56	Calicivirus	Calicivirus	NNP
cord-048471-7jszm1nd	215	57	Viral	viral	JJ
cord-048471-7jszm1nd	215	58	gastroenteritis	gastroenteritis	NN
cord-048471-7jszm1nd	215	59	outbreaks	outbreak	NNS
cord-048471-7jszm1nd	215	60	in	in	IN
cord-048471-7jszm1nd	215	61	Europe	Europe	NNP