id sid tid token lemma pos cord-001249-awn9ayy6 1 1 key key JJ cord-001249-awn9ayy6 2 1 : : : cord-001249-awn9ayy6 2 2 cord-001249-awn9ayy6 cord-001249-awn9ayy6 NNP cord-001249-awn9ayy6 2 3 authors author NNS cord-001249-awn9ayy6 2 4 : : : cord-001249-awn9ayy6 2 5 Lasecka Lasecka NNPS cord-001249-awn9ayy6 2 6 , , , cord-001249-awn9ayy6 2 7 Lidia Lidia NNP cord-001249-awn9ayy6 2 8 ; ; : cord-001249-awn9ayy6 2 9 Baron Baron NNP cord-001249-awn9ayy6 2 10 , , , cord-001249-awn9ayy6 2 11 Michael Michael NNP cord-001249-awn9ayy6 2 12 D. D. NNP cord-001249-awn9ayy6 2 13 title title NN cord-001249-awn9ayy6 2 14 : : : cord-001249-awn9ayy6 3 1 The the DT cord-001249-awn9ayy6 3 2 Nairovirus Nairovirus NNP cord-001249-awn9ayy6 3 3 Nairobi Nairobi NNP cord-001249-awn9ayy6 3 4 Sheep Sheep NNP cord-001249-awn9ayy6 3 5 Disease Disease NNP cord-001249-awn9ayy6 3 6 Virus Virus NNP cord-001249-awn9ayy6 3 7 / / SYM cord-001249-awn9ayy6 3 8 Ganjam Ganjam NNP cord-001249-awn9ayy6 3 9 Virus Virus NNP cord-001249-awn9ayy6 3 10 Induces induce VBZ cord-001249-awn9ayy6 3 11 the the DT cord-001249-awn9ayy6 3 12 Translocation Translocation NNP cord-001249-awn9ayy6 3 13 of of IN cord-001249-awn9ayy6 3 14 Protein Protein NNP cord-001249-awn9ayy6 3 15 Disulphide Disulphide NNP cord-001249-awn9ayy6 4 1 Isomerase isomerase JJ cord-001249-awn9ayy6 4 2 - - HYPH cord-001249-awn9ayy6 4 3 Like Like NNP cord-001249-awn9ayy6 4 4 Oxidoreductases Oxidoreductases NNP cord-001249-awn9ayy6 4 5 from from IN cord-001249-awn9ayy6 4 6 the the DT cord-001249-awn9ayy6 4 7 Endoplasmic Endoplasmic NNP cord-001249-awn9ayy6 4 8 Reticulum Reticulum NNP cord-001249-awn9ayy6 4 9 to to IN cord-001249-awn9ayy6 4 10 the the DT cord-001249-awn9ayy6 4 11 Cell Cell NNP cord-001249-awn9ayy6 4 12 Surface Surface NNP cord-001249-awn9ayy6 4 13 and and CC cord-001249-awn9ayy6 4 14 the the DT cord-001249-awn9ayy6 4 15 Extracellular Extracellular NNP cord-001249-awn9ayy6 4 16 Space Space NNP cord-001249-awn9ayy6 4 17 date date NN cord-001249-awn9ayy6 5 1 : : : cord-001249-awn9ayy6 5 2 2014 2014 CD cord-001249-awn9ayy6 5 3 - - HYPH cord-001249-awn9ayy6 5 4 04 04 CD cord-001249-awn9ayy6 5 5 - - HYPH cord-001249-awn9ayy6 5 6 08 08 CD cord-001249-awn9ayy6 5 7 journal journal NN cord-001249-awn9ayy6 5 8 : : : cord-001249-awn9ayy6 6 1 PLoS PLoS NNP cord-001249-awn9ayy6 6 2 One one CD cord-001249-awn9ayy6 6 3 DOI DOI NNP cord-001249-awn9ayy6 6 4 : : : cord-001249-awn9ayy6 6 5 10.1371 10.1371 CD cord-001249-awn9ayy6 6 6 / / SYM cord-001249-awn9ayy6 6 7 journal.pone.0094656 journal.pone.0094656 NN cord-001249-awn9ayy6 6 8 sha sha NNP cord-001249-awn9ayy6 6 9 : : : cord-001249-awn9ayy6 6 10 c6c0a0d35bdedb534df909619bd7f240f9d52ae6 c6c0a0d35bdedb534df909619bd7f240f9d52ae6 NNP cord-001249-awn9ayy6 6 11 doc_id doc_id CD cord-001249-awn9ayy6 6 12 : : : cord-001249-awn9ayy6 6 13 1249 1249 CD cord-001249-awn9ayy6 6 14 cord_uid cord_uid NNS cord-001249-awn9ayy6 6 15 : : : cord-001249-awn9ayy6 6 16 awn9ayy6 awn9ayy6 NNP cord-001249-awn9ayy6 7 1 Nairobi Nairobi NNP cord-001249-awn9ayy6 7 2 sheep sheep NN cord-001249-awn9ayy6 7 3 disease disease NN cord-001249-awn9ayy6 7 4 virus virus NN cord-001249-awn9ayy6 7 5 ( ( -LRB- cord-001249-awn9ayy6 7 6 NSDV NSDV NNP cord-001249-awn9ayy6 7 7 ) ) -RRB- cord-001249-awn9ayy6 7 8 of of IN cord-001249-awn9ayy6 7 9 the the DT cord-001249-awn9ayy6 7 10 genus genus NN cord-001249-awn9ayy6 7 11 Nairovirus Nairovirus NNP cord-001249-awn9ayy6 7 12 causes cause VBZ cord-001249-awn9ayy6 7 13 a a DT cord-001249-awn9ayy6 7 14 haemorrhagic haemorrhagic JJ cord-001249-awn9ayy6 7 15 gastroenteritis gastroenteritis NN cord-001249-awn9ayy6 7 16 in in IN cord-001249-awn9ayy6 7 17 sheep sheep NN cord-001249-awn9ayy6 7 18 and and CC cord-001249-awn9ayy6 7 19 goats goat NNS cord-001249-awn9ayy6 7 20 with with IN cord-001249-awn9ayy6 7 21 mortality mortality NN cord-001249-awn9ayy6 7 22 up up IN cord-001249-awn9ayy6 7 23 to to TO cord-001249-awn9ayy6 7 24 90 90 CD cord-001249-awn9ayy6 7 25 % % NN cord-001249-awn9ayy6 7 26 ; ; , cord-001249-awn9ayy6 7 27 the the DT cord-001249-awn9ayy6 7 28 virus virus NN cord-001249-awn9ayy6 7 29 is be VBZ cord-001249-awn9ayy6 7 30 found find VBN cord-001249-awn9ayy6 7 31 in in IN cord-001249-awn9ayy6 7 32 East East NNP cord-001249-awn9ayy6 7 33 and and CC cord-001249-awn9ayy6 7 34 Central Central NNP cord-001249-awn9ayy6 7 35 Africa Africa NNP cord-001249-awn9ayy6 7 36 , , , cord-001249-awn9ayy6 7 37 and and CC cord-001249-awn9ayy6 7 38 in in IN cord-001249-awn9ayy6 7 39 India India NNP cord-001249-awn9ayy6 7 40 , , , cord-001249-awn9ayy6 7 41 where where WRB cord-001249-awn9ayy6 7 42 the the DT cord-001249-awn9ayy6 7 43 virus virus NN cord-001249-awn9ayy6 7 44 is be VBZ cord-001249-awn9ayy6 7 45 called call VBN cord-001249-awn9ayy6 7 46 Ganjam Ganjam NNP cord-001249-awn9ayy6 7 47 virus virus NN cord-001249-awn9ayy6 7 48 . . . cord-001249-awn9ayy6 8 1 NSDV NSDV NNP cord-001249-awn9ayy6 8 2 is be VBZ cord-001249-awn9ayy6 8 3 closely closely RB cord-001249-awn9ayy6 8 4 related related JJ cord-001249-awn9ayy6 8 5 to to IN cord-001249-awn9ayy6 8 6 the the DT cord-001249-awn9ayy6 8 7 human human JJ cord-001249-awn9ayy6 8 8 pathogen pathogen NN cord-001249-awn9ayy6 8 9 Crimean Crimean NNP cord-001249-awn9ayy6 8 10 - - HYPH cord-001249-awn9ayy6 8 11 Congo Congo NNP cord-001249-awn9ayy6 8 12 haemorrhagic haemorrhagic JJ cord-001249-awn9ayy6 8 13 fever fever NN cord-001249-awn9ayy6 8 14 virus virus NN cord-001249-awn9ayy6 8 15 , , , cord-001249-awn9ayy6 8 16 which which WDT cord-001249-awn9ayy6 8 17 also also RB cord-001249-awn9ayy6 8 18 causes cause VBZ cord-001249-awn9ayy6 8 19 a a DT cord-001249-awn9ayy6 8 20 haemorrhagic haemorrhagic JJ cord-001249-awn9ayy6 8 21 disease disease NN cord-001249-awn9ayy6 8 22 . . . cord-001249-awn9ayy6 9 1 As as IN cord-001249-awn9ayy6 9 2 with with IN cord-001249-awn9ayy6 9 3 other other JJ cord-001249-awn9ayy6 9 4 nairoviruses nairoviruse NNS cord-001249-awn9ayy6 9 5 , , , cord-001249-awn9ayy6 9 6 replication replication NN cord-001249-awn9ayy6 9 7 of of IN cord-001249-awn9ayy6 9 8 NSDV NSDV NNP cord-001249-awn9ayy6 9 9 takes take VBZ cord-001249-awn9ayy6 9 10 place place NN cord-001249-awn9ayy6 9 11 in in IN cord-001249-awn9ayy6 9 12 the the DT cord-001249-awn9ayy6 9 13 cytoplasm cytoplasm NN cord-001249-awn9ayy6 9 14 and and CC cord-001249-awn9ayy6 9 15 the the DT cord-001249-awn9ayy6 9 16 new new JJ cord-001249-awn9ayy6 9 17 virus virus NN cord-001249-awn9ayy6 9 18 particles particle NNS cord-001249-awn9ayy6 9 19 bud bud NN cord-001249-awn9ayy6 9 20 into into IN cord-001249-awn9ayy6 9 21 the the DT cord-001249-awn9ayy6 9 22 Golgi Golgi NNP cord-001249-awn9ayy6 9 23 apparatus apparatus NN cord-001249-awn9ayy6 9 24 ; ; : cord-001249-awn9ayy6 9 25 however however RB cord-001249-awn9ayy6 9 26 , , , cord-001249-awn9ayy6 9 27 the the DT cord-001249-awn9ayy6 9 28 effect effect NN cord-001249-awn9ayy6 9 29 of of IN cord-001249-awn9ayy6 9 30 viral viral JJ cord-001249-awn9ayy6 9 31 replication replication NN cord-001249-awn9ayy6 9 32 on on IN cord-001249-awn9ayy6 9 33 cellular cellular JJ cord-001249-awn9ayy6 9 34 compartments compartment NNS cord-001249-awn9ayy6 9 35 has have VBZ cord-001249-awn9ayy6 9 36 not not RB cord-001249-awn9ayy6 9 37 been be VBN cord-001249-awn9ayy6 9 38 studied study VBN cord-001249-awn9ayy6 9 39 extensively extensively RB cord-001249-awn9ayy6 9 40 . . . cord-001249-awn9ayy6 10 1 We -PRON- PRP cord-001249-awn9ayy6 10 2 have have VBP cord-001249-awn9ayy6 10 3 found find VBN cord-001249-awn9ayy6 10 4 that that IN cord-001249-awn9ayy6 10 5 the the DT cord-001249-awn9ayy6 10 6 overall overall JJ cord-001249-awn9ayy6 10 7 structure structure NN cord-001249-awn9ayy6 10 8 of of IN cord-001249-awn9ayy6 10 9 the the DT cord-001249-awn9ayy6 10 10 endoplasmic endoplasmic NN cord-001249-awn9ayy6 10 11 reticulum reticulum NN cord-001249-awn9ayy6 10 12 ( ( -LRB- cord-001249-awn9ayy6 10 13 ER ER NNP cord-001249-awn9ayy6 10 14 ) ) -RRB- cord-001249-awn9ayy6 10 15 , , , cord-001249-awn9ayy6 10 16 the the DT cord-001249-awn9ayy6 10 17 ER ER NNP cord-001249-awn9ayy6 10 18 - - HYPH cord-001249-awn9ayy6 10 19 Golgi Golgi NNP cord-001249-awn9ayy6 10 20 intermediate intermediate JJ cord-001249-awn9ayy6 10 21 compartment compartment NN cord-001249-awn9ayy6 10 22 and and CC cord-001249-awn9ayy6 10 23 the the DT cord-001249-awn9ayy6 10 24 Golgi Golgi NNP cord-001249-awn9ayy6 10 25 were be VBD cord-001249-awn9ayy6 10 26 unaffected unaffected JJ cord-001249-awn9ayy6 10 27 by by IN cord-001249-awn9ayy6 10 28 infection infection NN cord-001249-awn9ayy6 10 29 with with IN cord-001249-awn9ayy6 10 30 NSDV NSDV NNP cord-001249-awn9ayy6 10 31 . . . cord-001249-awn9ayy6 11 1 However however RB cord-001249-awn9ayy6 11 2 , , , cord-001249-awn9ayy6 11 3 we -PRON- PRP cord-001249-awn9ayy6 11 4 observed observe VBD cord-001249-awn9ayy6 11 5 that that IN cord-001249-awn9ayy6 11 6 NSDV NSDV NNP cord-001249-awn9ayy6 11 7 infection infection NN cord-001249-awn9ayy6 11 8 led lead VBD cord-001249-awn9ayy6 11 9 to to IN cord-001249-awn9ayy6 11 10 the the DT cord-001249-awn9ayy6 11 11 loss loss NN cord-001249-awn9ayy6 11 12 of of IN cord-001249-awn9ayy6 11 13 protein protein NN cord-001249-awn9ayy6 11 14 disulphide disulphide NN cord-001249-awn9ayy6 11 15 isomerase isomerase NN cord-001249-awn9ayy6 11 16 ( ( -LRB- cord-001249-awn9ayy6 11 17 PDI PDI NNP cord-001249-awn9ayy6 11 18 ) ) -RRB- cord-001249-awn9ayy6 11 19 , , , cord-001249-awn9ayy6 11 20 an an DT cord-001249-awn9ayy6 11 21 oxidoreductase oxidoreductase NN cord-001249-awn9ayy6 11 22 present present NN cord-001249-awn9ayy6 11 23 in in IN cord-001249-awn9ayy6 11 24 the the DT cord-001249-awn9ayy6 11 25 lumen lumen NN cord-001249-awn9ayy6 11 26 of of IN cord-001249-awn9ayy6 11 27 the the DT cord-001249-awn9ayy6 11 28 endoplasmic endoplasmic NN cord-001249-awn9ayy6 11 29 reticulum reticulum NN cord-001249-awn9ayy6 11 30 ( ( -LRB- cord-001249-awn9ayy6 11 31 ER ER NNP cord-001249-awn9ayy6 11 32 ) ) -RRB- cord-001249-awn9ayy6 11 33 and and CC cord-001249-awn9ayy6 11 34 which which WDT cord-001249-awn9ayy6 11 35 assists assist VBZ cord-001249-awn9ayy6 11 36 during during IN cord-001249-awn9ayy6 11 37 protein protein NN cord-001249-awn9ayy6 11 38 folding folding NN cord-001249-awn9ayy6 11 39 , , , cord-001249-awn9ayy6 11 40 from from IN cord-001249-awn9ayy6 11 41 the the DT cord-001249-awn9ayy6 11 42 ER ER NNP cord-001249-awn9ayy6 11 43 . . . cord-001249-awn9ayy6 12 1 Further further JJ cord-001249-awn9ayy6 12 2 investigation investigation NN cord-001249-awn9ayy6 12 3 showed show VBD cord-001249-awn9ayy6 12 4 that that IN cord-001249-awn9ayy6 12 5 NSDV NSDV NNP cord-001249-awn9ayy6 12 6 - - HYPH cord-001249-awn9ayy6 12 7 infected infect VBN cord-001249-awn9ayy6 12 8 cells cell NNS cord-001249-awn9ayy6 12 9 have have VBP cord-001249-awn9ayy6 12 10 high high JJ cord-001249-awn9ayy6 12 11 levels level NNS cord-001249-awn9ayy6 12 12 of of IN cord-001249-awn9ayy6 12 13 PDI PDI NNP cord-001249-awn9ayy6 12 14 at at IN cord-001249-awn9ayy6 12 15 their -PRON- PRP$ cord-001249-awn9ayy6 12 16 surface surface NN cord-001249-awn9ayy6 12 17 , , , cord-001249-awn9ayy6 12 18 and and CC cord-001249-awn9ayy6 12 19 PDI PDI NNP cord-001249-awn9ayy6 12 20 is be VBZ cord-001249-awn9ayy6 12 21 also also RB cord-001249-awn9ayy6 12 22 secreted secrete VBN cord-001249-awn9ayy6 12 23 into into IN cord-001249-awn9ayy6 12 24 the the DT cord-001249-awn9ayy6 12 25 culture culture NN cord-001249-awn9ayy6 12 26 medium medium NN cord-001249-awn9ayy6 12 27 of of IN cord-001249-awn9ayy6 12 28 infected infected JJ cord-001249-awn9ayy6 12 29 cells cell NNS cord-001249-awn9ayy6 12 30 . . . cord-001249-awn9ayy6 13 1 Another another DT cord-001249-awn9ayy6 13 2 chaperone chaperone NN cord-001249-awn9ayy6 13 3 from from IN cord-001249-awn9ayy6 13 4 the the DT cord-001249-awn9ayy6 13 5 PDI PDI NNP cord-001249-awn9ayy6 13 6 family family NN cord-001249-awn9ayy6 13 7 , , , cord-001249-awn9ayy6 13 8 ERp57 ERp57 NNS cord-001249-awn9ayy6 13 9 , , , cord-001249-awn9ayy6 13 10 was be VBD cord-001249-awn9ayy6 13 11 found find VBN cord-001249-awn9ayy6 13 12 to to TO cord-001249-awn9ayy6 13 13 be be VB cord-001249-awn9ayy6 13 14 similarly similarly RB cord-001249-awn9ayy6 13 15 affected affect VBN cord-001249-awn9ayy6 13 16 . . . cord-001249-awn9ayy6 14 1 Analysis analysis NN cord-001249-awn9ayy6 14 2 of of IN cord-001249-awn9ayy6 14 3 infected infected JJ cord-001249-awn9ayy6 14 4 cells cell NNS cord-001249-awn9ayy6 14 5 and and CC cord-001249-awn9ayy6 14 6 expression expression NN cord-001249-awn9ayy6 14 7 of of IN cord-001249-awn9ayy6 14 8 individual individual JJ cord-001249-awn9ayy6 14 9 viral viral JJ cord-001249-awn9ayy6 14 10 glycoproteins glycoprotein NNS cord-001249-awn9ayy6 14 11 indicated indicate VBN cord-001249-awn9ayy6 14 12 that that IN cord-001249-awn9ayy6 14 13 the the DT cord-001249-awn9ayy6 14 14 NSDV NSDV NNP cord-001249-awn9ayy6 14 15 PreGn pregn NN cord-001249-awn9ayy6 14 16 glycoprotein glycoprotein NN cord-001249-awn9ayy6 14 17 is be VBZ cord-001249-awn9ayy6 14 18 involved involve VBN cord-001249-awn9ayy6 14 19 in in IN cord-001249-awn9ayy6 14 20 redistribution redistribution NN cord-001249-awn9ayy6 14 21 of of IN cord-001249-awn9ayy6 14 22 these these DT cord-001249-awn9ayy6 14 23 soluble soluble JJ cord-001249-awn9ayy6 14 24 ER er NN cord-001249-awn9ayy6 14 25 oxidoreductases oxidoreductase NNS cord-001249-awn9ayy6 14 26 . . . cord-001249-awn9ayy6 15 1 It -PRON- PRP cord-001249-awn9ayy6 15 2 has have VBZ cord-001249-awn9ayy6 15 3 been be VBN cord-001249-awn9ayy6 15 4 suggested suggest VBN cord-001249-awn9ayy6 15 5 that that IN cord-001249-awn9ayy6 15 6 extracellular extracellular JJ cord-001249-awn9ayy6 15 7 PDI PDI NNP cord-001249-awn9ayy6 15 8 can can MD cord-001249-awn9ayy6 15 9 activate activate VB cord-001249-awn9ayy6 15 10 integrins integrin NNS cord-001249-awn9ayy6 15 11 and and CC cord-001249-awn9ayy6 15 12 tissue tissue NN cord-001249-awn9ayy6 15 13 factor factor NN cord-001249-awn9ayy6 15 14 , , , cord-001249-awn9ayy6 15 15 which which WDT cord-001249-awn9ayy6 15 16 are be VBP cord-001249-awn9ayy6 15 17 involved involve VBN cord-001249-awn9ayy6 15 18 respectively respectively RB cord-001249-awn9ayy6 15 19 in in IN cord-001249-awn9ayy6 15 20 pro pro JJ cord-001249-awn9ayy6 15 21 - - JJ cord-001249-awn9ayy6 15 22 inflammatory inflammatory JJ cord-001249-awn9ayy6 15 23 responses response NNS cord-001249-awn9ayy6 15 24 and and CC cord-001249-awn9ayy6 15 25 disseminated disseminate VBN cord-001249-awn9ayy6 15 26 intravascular intravascular JJ cord-001249-awn9ayy6 15 27 coagulation coagulation NN cord-001249-awn9ayy6 15 28 , , , cord-001249-awn9ayy6 15 29 both both DT cord-001249-awn9ayy6 15 30 of of IN cord-001249-awn9ayy6 15 31 which which WDT cord-001249-awn9ayy6 15 32 manifest manifest VBP cord-001249-awn9ayy6 15 33 in in IN cord-001249-awn9ayy6 15 34 many many JJ cord-001249-awn9ayy6 15 35 viral viral JJ cord-001249-awn9ayy6 15 36 haemorrhagic haemorrhagic JJ cord-001249-awn9ayy6 15 37 fevers fever NNS cord-001249-awn9ayy6 15 38 . . . cord-001249-awn9ayy6 16 1 The the DT cord-001249-awn9ayy6 16 2 discovery discovery NN cord-001249-awn9ayy6 16 3 of of IN cord-001249-awn9ayy6 16 4 enhanced enhanced JJ cord-001249-awn9ayy6 16 5 PDI PDI NNP cord-001249-awn9ayy6 16 6 secretion secretion NN cord-001249-awn9ayy6 16 7 from from IN cord-001249-awn9ayy6 16 8 NSDV NSDV NNP cord-001249-awn9ayy6 16 9 - - HYPH cord-001249-awn9ayy6 16 10 infected infect VBN cord-001249-awn9ayy6 16 11 cells cell NNS cord-001249-awn9ayy6 16 12 may may MD cord-001249-awn9ayy6 16 13 be be VB cord-001249-awn9ayy6 16 14 an an DT cord-001249-awn9ayy6 16 15 important important JJ cord-001249-awn9ayy6 16 16 finding finding NN cord-001249-awn9ayy6 16 17 for for IN cord-001249-awn9ayy6 16 18 understanding understand VBG cord-001249-awn9ayy6 16 19 the the DT cord-001249-awn9ayy6 16 20 mechanisms mechanism NNS cord-001249-awn9ayy6 16 21 underlying underlie VBG cord-001249-awn9ayy6 16 22 the the DT cord-001249-awn9ayy6 16 23 pathogenicity pathogenicity NN cord-001249-awn9ayy6 16 24 of of IN cord-001249-awn9ayy6 16 25 haemorrhagic haemorrhagic JJ cord-001249-awn9ayy6 16 26 nairoviruses nairoviruse NNS cord-001249-awn9ayy6 16 27 . . . cord-001249-awn9ayy6 17 1 Nairobi Nairobi NNP cord-001249-awn9ayy6 17 2 sheep sheep NN cord-001249-awn9ayy6 17 3 disease disease NN cord-001249-awn9ayy6 17 4 virus virus NN cord-001249-awn9ayy6 17 5 ( ( -LRB- cord-001249-awn9ayy6 17 6 NSDV NSDV NNP cord-001249-awn9ayy6 17 7 ) ) -RRB- cord-001249-awn9ayy6 17 8 belongs belong VBZ cord-001249-awn9ayy6 17 9 to to TO cord-001249-awn9ayy6 17 10 genus genus VB cord-001249-awn9ayy6 17 11 Nairovirus Nairovirus NNP cord-001249-awn9ayy6 17 12 of of IN cord-001249-awn9ayy6 17 13 the the DT cord-001249-awn9ayy6 17 14 family family NN cord-001249-awn9ayy6 17 15 Bunyaviridae Bunyaviridae NNP cord-001249-awn9ayy6 17 16 and and CC cord-001249-awn9ayy6 17 17 causes cause VBZ cord-001249-awn9ayy6 17 18 a a DT cord-001249-awn9ayy6 17 19 severe severe JJ cord-001249-awn9ayy6 17 20 disease disease NN cord-001249-awn9ayy6 17 21 characterised characterise VBN cord-001249-awn9ayy6 17 22 by by IN cord-001249-awn9ayy6 17 23 fever fever NN cord-001249-awn9ayy6 17 24 and and CC cord-001249-awn9ayy6 17 25 haemorrhagic haemorrhagic JJ cord-001249-awn9ayy6 17 26 gastroenteritis gastroenteritis NN cord-001249-awn9ayy6 17 27 in in IN cord-001249-awn9ayy6 17 28 sheep sheep NN cord-001249-awn9ayy6 17 29 and and CC cord-001249-awn9ayy6 17 30 goats goat NNS cord-001249-awn9ayy6 17 31 with with IN cord-001249-awn9ayy6 17 32 a a DT cord-001249-awn9ayy6 17 33 mortality mortality NN cord-001249-awn9ayy6 17 34 rate rate NN cord-001249-awn9ayy6 17 35 up up IN cord-001249-awn9ayy6 17 36 to to TO cord-001249-awn9ayy6 17 37 90 90 CD cord-001249-awn9ayy6 17 38 % % NN cord-001249-awn9ayy6 17 39 in in IN cord-001249-awn9ayy6 17 40 a a DT cord-001249-awn9ayy6 17 41 susceptible susceptible JJ cord-001249-awn9ayy6 17 42 population population NN cord-001249-awn9ayy6 17 43 [ [ -LRB- cord-001249-awn9ayy6 17 44 1 1 CD cord-001249-awn9ayy6 17 45 , , , cord-001249-awn9ayy6 17 46 2 2 CD cord-001249-awn9ayy6 17 47 ] ] -RRB- cord-001249-awn9ayy6 17 48 . . . cord-001249-awn9ayy6 18 1 Nairobi Nairobi NNP cord-001249-awn9ayy6 18 2 sheep sheep NN cord-001249-awn9ayy6 18 3 disease disease NN cord-001249-awn9ayy6 18 4 was be VBD cord-001249-awn9ayy6 18 5 first first RB cord-001249-awn9ayy6 18 6 reported report VBN cord-001249-awn9ayy6 18 7 in in IN cord-001249-awn9ayy6 18 8 1910 1910 CD cord-001249-awn9ayy6 18 9 in in IN cord-001249-awn9ayy6 18 10 Nairobi Nairobi NNP cord-001249-awn9ayy6 18 11 , , , cord-001249-awn9ayy6 18 12 Kenya Kenya NNP cord-001249-awn9ayy6 18 13 and and CC cord-001249-awn9ayy6 18 14 , , , cord-001249-awn9ayy6 18 15 in in IN cord-001249-awn9ayy6 18 16 1917 1917 CD cord-001249-awn9ayy6 18 17 , , , cord-001249-awn9ayy6 18 18 NSDV NSDV NNP cord-001249-awn9ayy6 18 19 was be VBD cord-001249-awn9ayy6 18 20 shown show VBN cord-001249-awn9ayy6 18 21 to to TO cord-001249-awn9ayy6 18 22 be be VB cord-001249-awn9ayy6 18 23 the the DT cord-001249-awn9ayy6 18 24 causative causative JJ cord-001249-awn9ayy6 18 25 agent agent NN cord-001249-awn9ayy6 18 26 of of IN cord-001249-awn9ayy6 18 27 the the DT cord-001249-awn9ayy6 18 28 disease disease NN cord-001249-awn9ayy6 18 29 by by IN cord-001249-awn9ayy6 18 30 Montgomery Montgomery NNP cord-001249-awn9ayy6 18 31 and and CC cord-001249-awn9ayy6 18 32 colleagues colleague NNS cord-001249-awn9ayy6 18 33 [ [ -LRB- cord-001249-awn9ayy6 18 34 2 2 CD cord-001249-awn9ayy6 18 35 ] ] -RRB- cord-001249-awn9ayy6 18 36 . . . cord-001249-awn9ayy6 19 1 The the DT cord-001249-awn9ayy6 19 2 virus virus NN cord-001249-awn9ayy6 19 3 is be VBZ cord-001249-awn9ayy6 19 4 endemic endemic JJ cord-001249-awn9ayy6 19 5 in in IN cord-001249-awn9ayy6 19 6 East East NNP cord-001249-awn9ayy6 19 7 and and CC cord-001249-awn9ayy6 19 8 Central Central NNP cord-001249-awn9ayy6 19 9 Africa Africa NNP cord-001249-awn9ayy6 19 10 [ [ -LRB- cord-001249-awn9ayy6 19 11 2 2 CD cord-001249-awn9ayy6 19 12 ] ] -RRB- cord-001249-awn9ayy6 19 13 [ [ -LRB- cord-001249-awn9ayy6 19 14 3 3 CD cord-001249-awn9ayy6 19 15 ] ] -RRB- cord-001249-awn9ayy6 19 16 [ [ -LRB- cord-001249-awn9ayy6 19 17 4 4 CD cord-001249-awn9ayy6 19 18 ] ] -RRB- cord-001249-awn9ayy6 19 19 [ [ -LRB- cord-001249-awn9ayy6 19 20 5 5 CD cord-001249-awn9ayy6 19 21 ] ] -RRB- cord-001249-awn9ayy6 19 22 [ [ -LRB- cord-001249-awn9ayy6 19 23 6 6 CD cord-001249-awn9ayy6 19 24 ] ] -RRB- cord-001249-awn9ayy6 19 25 [ [ -LRB- cord-001249-awn9ayy6 19 26 7 7 CD cord-001249-awn9ayy6 19 27 ] ] -RRB- cord-001249-awn9ayy6 19 28 and and CC cord-001249-awn9ayy6 19 29 an an DT cord-001249-awn9ayy6 19 30 Asian asian JJ cord-001249-awn9ayy6 19 31 virus virus NN cord-001249-awn9ayy6 19 32 causing cause VBG cord-001249-awn9ayy6 19 33 the the DT cord-001249-awn9ayy6 19 34 same same JJ cord-001249-awn9ayy6 19 35 disease disease NN cord-001249-awn9ayy6 19 36 in in IN cord-001249-awn9ayy6 19 37 India India NNP cord-001249-awn9ayy6 19 38 is be VBZ cord-001249-awn9ayy6 19 39 called call VBN cord-001249-awn9ayy6 19 40 Ganjam Ganjam NNP cord-001249-awn9ayy6 19 41 virus virus NN cord-001249-awn9ayy6 19 42 ( ( -LRB- cord-001249-awn9ayy6 19 43 GV GV NNP cord-001249-awn9ayy6 19 44 ) ) -RRB- cord-001249-awn9ayy6 19 45 ( ( -LRB- cord-001249-awn9ayy6 19 46 reviewed review VBN cord-001249-awn9ayy6 19 47 in in IN cord-001249-awn9ayy6 19 48 [ [ -LRB- cord-001249-awn9ayy6 19 49 8 8 CD cord-001249-awn9ayy6 19 50 ] ] -RRB- cord-001249-awn9ayy6 19 51 ) ) -RRB- cord-001249-awn9ayy6 19 52 . . . cord-001249-awn9ayy6 20 1 Based base VBN cord-001249-awn9ayy6 20 2 on on IN cord-001249-awn9ayy6 20 3 genetic genetic JJ cord-001249-awn9ayy6 20 4 and and CC cord-001249-awn9ayy6 20 5 serological serological JJ cord-001249-awn9ayy6 20 6 studies study NNS cord-001249-awn9ayy6 20 7 , , , cord-001249-awn9ayy6 20 8 these these DT cord-001249-awn9ayy6 20 9 viruses virus NNS cord-001249-awn9ayy6 20 10 were be VBD cord-001249-awn9ayy6 20 11 identified identify VBN cord-001249-awn9ayy6 20 12 as as IN cord-001249-awn9ayy6 20 13 different different JJ cord-001249-awn9ayy6 20 14 isolates isolate NNS cord-001249-awn9ayy6 20 15 of of IN cord-001249-awn9ayy6 20 16 the the DT cord-001249-awn9ayy6 20 17 same same JJ cord-001249-awn9ayy6 20 18 virus virus NN cord-001249-awn9ayy6 20 19 [ [ -LRB- cord-001249-awn9ayy6 20 20 9 9 CD cord-001249-awn9ayy6 20 21 , , , cord-001249-awn9ayy6 20 22 10 10 CD cord-001249-awn9ayy6 20 23 ] ] -RRB- cord-001249-awn9ayy6 20 24 . . . cord-001249-awn9ayy6 21 1 NSDV NSDV NNP cord-001249-awn9ayy6 21 2 does do VBZ cord-001249-awn9ayy6 21 3 not not RB cord-001249-awn9ayy6 21 4 appear appear VB cord-001249-awn9ayy6 21 5 to to TO cord-001249-awn9ayy6 21 6 be be VB cord-001249-awn9ayy6 21 7 contagious contagious JJ cord-001249-awn9ayy6 21 8 and and CC cord-001249-awn9ayy6 21 9 the the DT cord-001249-awn9ayy6 21 10 virus virus NN cord-001249-awn9ayy6 21 11 needs need VBZ cord-001249-awn9ayy6 21 12 to to TO cord-001249-awn9ayy6 21 13 be be VB cord-001249-awn9ayy6 21 14 transmitted transmit VBN cord-001249-awn9ayy6 21 15 by by IN cord-001249-awn9ayy6 21 16 ticks tick NNS cord-001249-awn9ayy6 21 17 in in IN cord-001249-awn9ayy6 21 18 natural natural JJ cord-001249-awn9ayy6 21 19 infection infection NN cord-001249-awn9ayy6 21 20 [ [ -LRB- cord-001249-awn9ayy6 21 21 2 2 CD cord-001249-awn9ayy6 21 22 ] ] -RRB- cord-001249-awn9ayy6 21 23 . . . cord-001249-awn9ayy6 22 1 The the DT cord-001249-awn9ayy6 22 2 virus virus NN cord-001249-awn9ayy6 22 3 is be VBZ cord-001249-awn9ayy6 22 4 primarily primarily RB cord-001249-awn9ayy6 22 5 transmitted transmit VBN cord-001249-awn9ayy6 22 6 by by IN cord-001249-awn9ayy6 22 7 hard hard JJ cord-001249-awn9ayy6 22 8 ( ( -LRB- cord-001249-awn9ayy6 22 9 Ixodid ixodid NN cord-001249-awn9ayy6 22 10 ) ) -RRB- cord-001249-awn9ayy6 22 11 ticks tick NNS cord-001249-awn9ayy6 22 12 , , , cord-001249-awn9ayy6 22 13 with with IN cord-001249-awn9ayy6 22 14 Rhipicephalus Rhipicephalus NNP cord-001249-awn9ayy6 22 15 appendiculatus appendiculatus NNP cord-001249-awn9ayy6 22 16 being be VBG cord-001249-awn9ayy6 22 17 the the DT cord-001249-awn9ayy6 22 18 main main JJ cord-001249-awn9ayy6 22 19 vector vector NN cord-001249-awn9ayy6 22 20 in in IN cord-001249-awn9ayy6 22 21 Africa Africa NNP cord-001249-awn9ayy6 22 22 [ [ -LRB- cord-001249-awn9ayy6 22 23 4 4 CD cord-001249-awn9ayy6 22 24 ] ] -RRB- cord-001249-awn9ayy6 22 25 and and CC cord-001249-awn9ayy6 22 26 Haemaphysalis Haemaphysalis NNP cord-001249-awn9ayy6 22 27 intermedia intermedia NN cord-001249-awn9ayy6 23 1 the the DT cord-001249-awn9ayy6 23 2 main main JJ cord-001249-awn9ayy6 23 3 vector vector NN cord-001249-awn9ayy6 23 4 in in IN cord-001249-awn9ayy6 23 5 India India NNP cord-001249-awn9ayy6 23 6 [ [ -LRB- cord-001249-awn9ayy6 23 7 11 11 CD cord-001249-awn9ayy6 23 8 ] ] -RRB- cord-001249-awn9ayy6 23 9 [ [ -LRB- cord-001249-awn9ayy6 23 10 12 12 CD cord-001249-awn9ayy6 23 11 ] ] -RRB- cord-001249-awn9ayy6 23 12 [ [ -LRB- cord-001249-awn9ayy6 23 13 13 13 CD cord-001249-awn9ayy6 23 14 ] ] -RRB- cord-001249-awn9ayy6 23 15 . . . cord-001249-awn9ayy6 24 1 Sheep Sheep NNS cord-001249-awn9ayy6 24 2 and and CC cord-001249-awn9ayy6 24 3 goats goat NNS cord-001249-awn9ayy6 24 4 are be VBP cord-001249-awn9ayy6 24 5 the the DT cord-001249-awn9ayy6 24 6 only only RB cord-001249-awn9ayy6 24 7 known know VBN cord-001249-awn9ayy6 24 8 mammalian mammalian JJ cord-001249-awn9ayy6 24 9 reservoir reservoir NN cord-001249-awn9ayy6 24 10 for for IN cord-001249-awn9ayy6 24 11 NSDV NSDV NNP cord-001249-awn9ayy6 24 12 [ [ -LRB- cord-001249-awn9ayy6 24 13 3 3 CD cord-001249-awn9ayy6 24 14 , , , cord-001249-awn9ayy6 24 15 4 4 CD cord-001249-awn9ayy6 24 16 ] ] -RRB- cord-001249-awn9ayy6 24 17 ; ; : cord-001249-awn9ayy6 24 18 other other JJ cord-001249-awn9ayy6 24 19 livestock livestock NN cord-001249-awn9ayy6 24 20 ( ( -LRB- cord-001249-awn9ayy6 24 21 e.g. e.g. JJ cord-001249-awn9ayy6 24 22 cattle cattle NNS cord-001249-awn9ayy6 24 23 , , , cord-001249-awn9ayy6 24 24 horses horse NNS cord-001249-awn9ayy6 24 25 ) ) -RRB- cord-001249-awn9ayy6 24 26 are be VBP cord-001249-awn9ayy6 24 27 refractory refractory JJ cord-001249-awn9ayy6 24 28 to to IN cord-001249-awn9ayy6 24 29 the the DT cord-001249-awn9ayy6 24 30 disease disease NN cord-001249-awn9ayy6 24 31 [ [ -LRB- cord-001249-awn9ayy6 24 32 2 2 CD cord-001249-awn9ayy6 24 33 ] ] -RRB- cord-001249-awn9ayy6 24 34 . . . cord-001249-awn9ayy6 25 1 While while IN cord-001249-awn9ayy6 25 2 the the DT cord-001249-awn9ayy6 25 3 virus virus NN cord-001249-awn9ayy6 25 4 has have VBZ cord-001249-awn9ayy6 25 5 a a DT cord-001249-awn9ayy6 25 6 limited limited JJ cord-001249-awn9ayy6 25 7 effect effect NN cord-001249-awn9ayy6 25 8 on on IN cord-001249-awn9ayy6 25 9 animals animal NNS cord-001249-awn9ayy6 25 10 bred breed VBN cord-001249-awn9ayy6 25 11 in in IN cord-001249-awn9ayy6 25 12 the the DT cord-001249-awn9ayy6 25 13 enzootic enzootic JJ cord-001249-awn9ayy6 25 14 areas area NNS cord-001249-awn9ayy6 25 15 due due JJ cord-001249-awn9ayy6 25 16 to to IN cord-001249-awn9ayy6 25 17 the the DT cord-001249-awn9ayy6 25 18 development development NN cord-001249-awn9ayy6 25 19 of of IN cord-001249-awn9ayy6 25 20 immunity immunity NN cord-001249-awn9ayy6 25 21 by by IN cord-001249-awn9ayy6 25 22 these these DT cord-001249-awn9ayy6 25 23 animals animal NNS cord-001249-awn9ayy6 25 24 while while IN cord-001249-awn9ayy6 25 25 they -PRON- PRP cord-001249-awn9ayy6 25 26 are be VBP cord-001249-awn9ayy6 25 27 still still RB cord-001249-awn9ayy6 25 28 protected protect VBN cord-001249-awn9ayy6 25 29 by by IN cord-001249-awn9ayy6 25 30 maternal maternal JJ cord-001249-awn9ayy6 25 31 antibodies antibody NNS cord-001249-awn9ayy6 25 32 , , , cord-001249-awn9ayy6 25 33 NSDV NSDV NNP cord-001249-awn9ayy6 25 34 causes cause VBZ cord-001249-awn9ayy6 25 35 large large JJ cord-001249-awn9ayy6 25 36 economic economic JJ cord-001249-awn9ayy6 25 37 losses loss NNS cord-001249-awn9ayy6 25 38 during during IN cord-001249-awn9ayy6 25 39 transport transport NN cord-001249-awn9ayy6 25 40 of of IN cord-001249-awn9ayy6 25 41 animals animal NNS cord-001249-awn9ayy6 25 42 through through IN cord-001249-awn9ayy6 25 43 enzootic enzootic JJ cord-001249-awn9ayy6 25 44 areas area NNS cord-001249-awn9ayy6 25 45 or or CC cord-001249-awn9ayy6 25 46 during during IN cord-001249-awn9ayy6 25 47 introduction introduction NN cord-001249-awn9ayy6 25 48 of of IN cord-001249-awn9ayy6 25 49 new new JJ cord-001249-awn9ayy6 25 50 livestock livestock NN cord-001249-awn9ayy6 25 51 to to IN cord-001249-awn9ayy6 25 52 these these DT cord-001249-awn9ayy6 25 53 areas area NNS cord-001249-awn9ayy6 25 54 [ [ -LRB- cord-001249-awn9ayy6 25 55 2 2 CD cord-001249-awn9ayy6 25 56 , , , cord-001249-awn9ayy6 25 57 [ [ -LRB- cord-001249-awn9ayy6 25 58 14 14 CD cord-001249-awn9ayy6 25 59 ] ] -RRB- cord-001249-awn9ayy6 25 60 [ [ -LRB- cord-001249-awn9ayy6 25 61 15 15 CD cord-001249-awn9ayy6 25 62 ] ] -RRB- cord-001249-awn9ayy6 25 63 [ [ -LRB- cord-001249-awn9ayy6 25 64 16 16 CD cord-001249-awn9ayy6 25 65 ] ] -RRB- cord-001249-awn9ayy6 25 66 . . . cord-001249-awn9ayy6 26 1 Currently currently RB cord-001249-awn9ayy6 26 2 there there EX cord-001249-awn9ayy6 26 3 is be VBZ cord-001249-awn9ayy6 26 4 no no DT cord-001249-awn9ayy6 26 5 safe safe JJ cord-001249-awn9ayy6 26 6 vaccine vaccine NN cord-001249-awn9ayy6 26 7 [ [ -LRB- cord-001249-awn9ayy6 26 8 17 17 CD cord-001249-awn9ayy6 26 9 ] ] -RRB- cord-001249-awn9ayy6 26 10 . . . cord-001249-awn9ayy6 27 1 NSDV NSDV NNP cord-001249-awn9ayy6 27 2 is be VBZ cord-001249-awn9ayy6 27 3 closely closely RB cord-001249-awn9ayy6 27 4 related related JJ cord-001249-awn9ayy6 27 5 to to IN cord-001249-awn9ayy6 27 6 a a DT cord-001249-awn9ayy6 27 7 human human JJ cord-001249-awn9ayy6 27 8 pathogen pathogen NN cord-001249-awn9ayy6 27 9 , , , cord-001249-awn9ayy6 27 10 Crimean Crimean NNP cord-001249-awn9ayy6 27 11 - - HYPH cord-001249-awn9ayy6 27 12 Congo Congo NNP cord-001249-awn9ayy6 27 13 haemorrhagic haemorrhagic JJ cord-001249-awn9ayy6 27 14 fever fever NN cord-001249-awn9ayy6 27 15 virus virus NN cord-001249-awn9ayy6 27 16 ( ( -LRB- cord-001249-awn9ayy6 27 17 CCHFV CCHFV NNP cord-001249-awn9ayy6 27 18 ) ) -RRB- cord-001249-awn9ayy6 27 19 , , , cord-001249-awn9ayy6 27 20 which which WDT cord-001249-awn9ayy6 27 21 causes cause VBZ cord-001249-awn9ayy6 27 22 viral viral JJ cord-001249-awn9ayy6 27 23 haemorrhagic haemorrhagic JJ cord-001249-awn9ayy6 27 24 fever fever NN cord-001249-awn9ayy6 27 25 with with IN cord-001249-awn9ayy6 27 26 an an DT cord-001249-awn9ayy6 27 27 average average JJ cord-001249-awn9ayy6 27 28 mortality mortality NN cord-001249-awn9ayy6 27 29 rate rate NN cord-001249-awn9ayy6 27 30 of of IN cord-001249-awn9ayy6 27 31 30 30 CD cord-001249-awn9ayy6 27 32 % % NN cord-001249-awn9ayy6 27 33 ( ( -LRB- cord-001249-awn9ayy6 27 34 reviewed review VBN cord-001249-awn9ayy6 27 35 in in IN cord-001249-awn9ayy6 27 36 [ [ -LRB- cord-001249-awn9ayy6 27 37 18 18 CD cord-001249-awn9ayy6 27 38 , , , cord-001249-awn9ayy6 27 39 19 19 CD cord-001249-awn9ayy6 27 40 ] ] -RRB- cord-001249-awn9ayy6 27 41 ) ) -RRB- cord-001249-awn9ayy6 27 42 . . . cord-001249-awn9ayy6 28 1 After after IN cord-001249-awn9ayy6 28 2 Dengue Dengue NNP cord-001249-awn9ayy6 28 3 virus virus NN cord-001249-awn9ayy6 28 4 ( ( -LRB- cord-001249-awn9ayy6 28 5 DENV DENV NNP cord-001249-awn9ayy6 28 6 ) ) -RRB- cord-001249-awn9ayy6 28 7 , , , cord-001249-awn9ayy6 28 8 CCHFV CCHFV NNP cord-001249-awn9ayy6 28 9 is be VBZ cord-001249-awn9ayy6 28 10 the the DT cord-001249-awn9ayy6 28 11 second second JJ cord-001249-awn9ayy6 28 12 most most RBS cord-001249-awn9ayy6 28 13 widespread widespread JJ cord-001249-awn9ayy6 28 14 of of IN cord-001249-awn9ayy6 28 15 the the DT cord-001249-awn9ayy6 28 16 arboviruses arbovirus NNS cord-001249-awn9ayy6 28 17 pathogenic pathogenic JJ cord-001249-awn9ayy6 28 18 to to IN cord-001249-awn9ayy6 28 19 humans human NNS cord-001249-awn9ayy6 28 20 [ [ -LRB- cord-001249-awn9ayy6 28 21 20 20 CD cord-001249-awn9ayy6 28 22 ] ] -RRB- cord-001249-awn9ayy6 28 23 [ [ -LRB- cord-001249-awn9ayy6 28 24 21 21 CD cord-001249-awn9ayy6 28 25 ] ] -RRB- cord-001249-awn9ayy6 29 1 [ [ -LRB- cord-001249-awn9ayy6 29 2 22 22 CD cord-001249-awn9ayy6 29 3 ] ] -RRB- cord-001249-awn9ayy6 29 4 [ [ -LRB- cord-001249-awn9ayy6 29 5 23 23 CD cord-001249-awn9ayy6 29 6 ] ] -RRB- cord-001249-awn9ayy6 30 1 [ [ -LRB- cord-001249-awn9ayy6 30 2 24 24 CD cord-001249-awn9ayy6 30 3 ] ] -RRB- cord-001249-awn9ayy6 30 4 [ [ -LRB- cord-001249-awn9ayy6 30 5 25 25 CD cord-001249-awn9ayy6 30 6 ] ] -RRB- cord-001249-awn9ayy6 30 7 [ [ -LRB- cord-001249-awn9ayy6 30 8 26 26 CD cord-001249-awn9ayy6 30 9 ] ] -RRB- cord-001249-awn9ayy6 30 10 [ [ -LRB- cord-001249-awn9ayy6 30 11 27 27 CD cord-001249-awn9ayy6 30 12 ] ] -RRB- cord-001249-awn9ayy6 30 13 [ [ -LRB- cord-001249-awn9ayy6 30 14 28 28 CD cord-001249-awn9ayy6 30 15 ] ] -RRB- cord-001249-awn9ayy6 30 16 . . . cord-001249-awn9ayy6 31 1 The the DT cord-001249-awn9ayy6 31 2 disease disease NN cord-001249-awn9ayy6 31 3 caused cause VBN cord-001249-awn9ayy6 31 4 by by IN cord-001249-awn9ayy6 31 5 CCHFV CCHFV NNP cord-001249-awn9ayy6 31 6 in in IN cord-001249-awn9ayy6 31 7 humans human NNS cord-001249-awn9ayy6 31 8 is be VBZ cord-001249-awn9ayy6 31 9 similar similar JJ cord-001249-awn9ayy6 31 10 to to IN cord-001249-awn9ayy6 31 11 that that DT cord-001249-awn9ayy6 31 12 caused cause VBN cord-001249-awn9ayy6 31 13 in in IN cord-001249-awn9ayy6 31 14 sheep sheep NNS cord-001249-awn9ayy6 31 15 and and CC cord-001249-awn9ayy6 31 16 goats goat NNS cord-001249-awn9ayy6 31 17 by by IN cord-001249-awn9ayy6 31 18 NSDV NSDV NNP cord-001249-awn9ayy6 31 19 infection infection NN cord-001249-awn9ayy6 31 20 [ [ -LRB- cord-001249-awn9ayy6 31 21 2 2 CD cord-001249-awn9ayy6 31 22 , , , cord-001249-awn9ayy6 31 23 29 29 CD cord-001249-awn9ayy6 31 24 ] ] -RRB- cord-001249-awn9ayy6 31 25 , , , cord-001249-awn9ayy6 31 26 and and CC cord-001249-awn9ayy6 31 27 is be VBZ cord-001249-awn9ayy6 31 28 characterised characterise VBN cord-001249-awn9ayy6 31 29 by by IN cord-001249-awn9ayy6 31 30 fever fever NN cord-001249-awn9ayy6 31 31 , , , cord-001249-awn9ayy6 31 32 myalgia myalgia NN cord-001249-awn9ayy6 31 33 , , , cord-001249-awn9ayy6 31 34 superficial superficial JJ cord-001249-awn9ayy6 31 35 and and CC cord-001249-awn9ayy6 31 36 internal internal JJ cord-001249-awn9ayy6 31 37 haemorrhage haemorrhage NN cord-001249-awn9ayy6 31 38 , , , cord-001249-awn9ayy6 31 39 abdominal abdominal JJ cord-001249-awn9ayy6 31 40 pain pain NN cord-001249-awn9ayy6 31 41 and and CC cord-001249-awn9ayy6 31 42 diarrhoea diarrhoea NN cord-001249-awn9ayy6 31 43 [ [ -LRB- cord-001249-awn9ayy6 31 44 30 30 CD cord-001249-awn9ayy6 31 45 ] ] -RRB- cord-001249-awn9ayy6 31 46 [ [ -LRB- cord-001249-awn9ayy6 31 47 31 31 CD cord-001249-awn9ayy6 31 48 ] ] -RRB- cord-001249-awn9ayy6 31 49 [ [ -LRB- cord-001249-awn9ayy6 31 50 32 32 CD cord-001249-awn9ayy6 31 51 ] ] -RRB- cord-001249-awn9ayy6 31 52 [ [ -LRB- cord-001249-awn9ayy6 31 53 33 33 CD cord-001249-awn9ayy6 31 54 ] ] -RRB- cord-001249-awn9ayy6 31 55 . . . cord-001249-awn9ayy6 32 1 While while IN cord-001249-awn9ayy6 32 2 work work NN cord-001249-awn9ayy6 32 3 on on IN cord-001249-awn9ayy6 32 4 CCHFV CCHFV NNP cord-001249-awn9ayy6 32 5 is be VBZ cord-001249-awn9ayy6 32 6 limited limit VBN cord-001249-awn9ayy6 32 7 to to IN cord-001249-awn9ayy6 32 8 biosafety biosafety NN cord-001249-awn9ayy6 32 9 level level NN cord-001249-awn9ayy6 32 10 ( ( -LRB- cord-001249-awn9ayy6 32 11 BSL BSL NNP cord-001249-awn9ayy6 32 12 ) ) -RRB- cord-001249-awn9ayy6 32 13 4 4 CD cord-001249-awn9ayy6 32 14 laboratories laboratory NNS cord-001249-awn9ayy6 32 15 and and CC cord-001249-awn9ayy6 32 16 restricted restrict VBN cord-001249-awn9ayy6 32 17 by by IN cord-001249-awn9ayy6 32 18 lack lack NN cord-001249-awn9ayy6 32 19 of of IN cord-001249-awn9ayy6 32 20 a a DT cord-001249-awn9ayy6 32 21 natural natural JJ cord-001249-awn9ayy6 32 22 animal animal NN cord-001249-awn9ayy6 32 23 model model NN cord-001249-awn9ayy6 32 24 to to TO cord-001249-awn9ayy6 32 25 study study VB cord-001249-awn9ayy6 32 26 the the DT cord-001249-awn9ayy6 32 27 disease disease NN cord-001249-awn9ayy6 32 28 , , , cord-001249-awn9ayy6 32 29 NSDV NSDV NNP cord-001249-awn9ayy6 32 30 may may MD cord-001249-awn9ayy6 32 31 act act VB cord-001249-awn9ayy6 32 32 as as IN cord-001249-awn9ayy6 32 33 a a DT cord-001249-awn9ayy6 32 34 suitable suitable JJ cord-001249-awn9ayy6 32 35 model model NN cord-001249-awn9ayy6 32 36 to to TO cord-001249-awn9ayy6 32 37 study study VB cord-001249-awn9ayy6 32 38 haemorrhagic haemorrhagic JJ cord-001249-awn9ayy6 32 39 nairoviruses nairoviruse NNS cord-001249-awn9ayy6 32 40 . . . cord-001249-awn9ayy6 33 1 Nairoviruses nairoviruse NNS cord-001249-awn9ayy6 33 2 are be VBP cord-001249-awn9ayy6 33 3 enveloped envelop VBN cord-001249-awn9ayy6 33 4 viruses virus NNS cord-001249-awn9ayy6 33 5 which which WDT cord-001249-awn9ayy6 33 6 appear appear VBP cord-001249-awn9ayy6 33 7 spherical spherical JJ cord-001249-awn9ayy6 33 8 in in IN cord-001249-awn9ayy6 33 9 the the DT cord-001249-awn9ayy6 33 10 electron electron NN cord-001249-awn9ayy6 33 11 microscope microscope NN cord-001249-awn9ayy6 33 12 , , , cord-001249-awn9ayy6 33 13 with with IN cord-001249-awn9ayy6 33 14 a a DT cord-001249-awn9ayy6 33 15 diameter diameter NN cord-001249-awn9ayy6 33 16 of of IN cord-001249-awn9ayy6 33 17 approximately approximately RB cord-001249-awn9ayy6 33 18 100 100 CD cord-001249-awn9ayy6 33 19 nm nm CD cord-001249-awn9ayy6 33 20 [ [ -LRB- cord-001249-awn9ayy6 33 21 34 34 CD cord-001249-awn9ayy6 33 22 ] ] -RRB- cord-001249-awn9ayy6 33 23 [ [ -LRB- cord-001249-awn9ayy6 33 24 35 35 CD cord-001249-awn9ayy6 33 25 ] ] -RRB- cord-001249-awn9ayy6 33 26 [ [ -LRB- cord-001249-awn9ayy6 33 27 36 36 CD cord-001249-awn9ayy6 33 28 ] ] -RRB- cord-001249-awn9ayy6 33 29 ; ; : cord-001249-awn9ayy6 33 30 the the DT cord-001249-awn9ayy6 33 31 viral viral JJ cord-001249-awn9ayy6 33 32 genome genome NN cord-001249-awn9ayy6 33 33 consists consist VBZ cord-001249-awn9ayy6 33 34 of of IN cord-001249-awn9ayy6 33 35 three three CD cord-001249-awn9ayy6 33 36 negative negative JJ cord-001249-awn9ayy6 33 37 - - HYPH cord-001249-awn9ayy6 33 38 sense sense NN cord-001249-awn9ayy6 33 39 RNA RNA NNP cord-001249-awn9ayy6 33 40 segments segment NNS cord-001249-awn9ayy6 33 41 [ [ -LRB- cord-001249-awn9ayy6 33 42 37 37 CD cord-001249-awn9ayy6 33 43 ] ] -RRB- cord-001249-awn9ayy6 34 1 [ [ -LRB- cord-001249-awn9ayy6 34 2 38 38 CD cord-001249-awn9ayy6 34 3 ] ] -RRB- cord-001249-awn9ayy6 35 1 [ [ -LRB- cord-001249-awn9ayy6 35 2 39 39 CD cord-001249-awn9ayy6 35 3 ] ] -RRB- cord-001249-awn9ayy6 35 4 which which WDT cord-001249-awn9ayy6 35 5 are be VBP cord-001249-awn9ayy6 35 6 encapsidated encapsidate VBN cord-001249-awn9ayy6 35 7 by by IN cord-001249-awn9ayy6 35 8 the the DT cord-001249-awn9ayy6 35 9 viral viral JJ cord-001249-awn9ayy6 35 10 nucleoprotein nucleoprotein NN cord-001249-awn9ayy6 35 11 forming form VBG cord-001249-awn9ayy6 35 12 , , , cord-001249-awn9ayy6 35 13 together together RB cord-001249-awn9ayy6 35 14 with with IN cord-001249-awn9ayy6 35 15 the the DT cord-001249-awn9ayy6 35 16 viral viral JJ cord-001249-awn9ayy6 35 17 RNA RNA NNP cord-001249-awn9ayy6 35 18 , , , cord-001249-awn9ayy6 35 19 the the DT cord-001249-awn9ayy6 35 20 ribonucleoprotein ribonucleoprotein NN cord-001249-awn9ayy6 35 21 ( ( -LRB- cord-001249-awn9ayy6 35 22 RNP RNP NNP cord-001249-awn9ayy6 35 23 ) ) -RRB- cord-001249-awn9ayy6 36 1 [ [ -LRB- cord-001249-awn9ayy6 36 2 37 37 CD cord-001249-awn9ayy6 36 3 , , , cord-001249-awn9ayy6 36 4 [ [ -LRB- cord-001249-awn9ayy6 36 5 40 40 CD cord-001249-awn9ayy6 36 6 ] ] -RRB- cord-001249-awn9ayy6 36 7 [ [ -LRB- cord-001249-awn9ayy6 36 8 41 41 CD cord-001249-awn9ayy6 36 9 ] ] -RRB- cord-001249-awn9ayy6 36 10 [ [ -LRB- cord-001249-awn9ayy6 36 11 42 42 CD cord-001249-awn9ayy6 36 12 ] ] -RRB- cord-001249-awn9ayy6 36 13 [ [ -LRB- cord-001249-awn9ayy6 36 14 43 43 CD cord-001249-awn9ayy6 36 15 ] ] -RRB- cord-001249-awn9ayy6 36 16 [ [ -LRB- cord-001249-awn9ayy6 36 17 44 44 CD cord-001249-awn9ayy6 36 18 ] ] -RRB- cord-001249-awn9ayy6 36 19 [ [ -LRB- cord-001249-awn9ayy6 36 20 45 45 CD cord-001249-awn9ayy6 36 21 ] ] -RRB- cord-001249-awn9ayy6 36 22 . . . cord-001249-awn9ayy6 37 1 The the DT cord-001249-awn9ayy6 37 2 three three CD cord-001249-awn9ayy6 37 3 RNA RNA NNP cord-001249-awn9ayy6 37 4 segments segment NNS cord-001249-awn9ayy6 37 5 are be VBP cord-001249-awn9ayy6 37 6 called call VBN cord-001249-awn9ayy6 37 7 small small JJ cord-001249-awn9ayy6 37 8 ( ( -LRB- cord-001249-awn9ayy6 37 9 S S NNP cord-001249-awn9ayy6 37 10 ) ) -RRB- cord-001249-awn9ayy6 37 11 , , , cord-001249-awn9ayy6 37 12 medium medium NN cord-001249-awn9ayy6 37 13 ( ( -LRB- cord-001249-awn9ayy6 37 14 M M NNP cord-001249-awn9ayy6 37 15 ) ) -RRB- cord-001249-awn9ayy6 37 16 and and CC cord-001249-awn9ayy6 37 17 large large JJ cord-001249-awn9ayy6 37 18 ( ( -LRB- cord-001249-awn9ayy6 37 19 L l NN cord-001249-awn9ayy6 37 20 ) ) -RRB- cord-001249-awn9ayy6 37 21 and and CC cord-001249-awn9ayy6 37 22 encode encode VB cord-001249-awn9ayy6 37 23 respectively respectively RB cord-001249-awn9ayy6 37 24 the the DT cord-001249-awn9ayy6 37 25 nucleoprotein nucleoprotein NNP cord-001249-awn9ayy6 37 26 ( ( -LRB- cord-001249-awn9ayy6 37 27 N N NNP cord-001249-awn9ayy6 37 28 ) ) -RRB- cord-001249-awn9ayy6 37 29 [ [ -LRB- cord-001249-awn9ayy6 37 30 37 37 CD cord-001249-awn9ayy6 37 31 , , , cord-001249-awn9ayy6 37 32 42 42 CD cord-001249-awn9ayy6 37 33 , , , cord-001249-awn9ayy6 37 34 46 46 CD cord-001249-awn9ayy6 37 35 ] ] -RRB- cord-001249-awn9ayy6 37 36 , , , cord-001249-awn9ayy6 37 37 the the DT cord-001249-awn9ayy6 37 38 viral viral JJ cord-001249-awn9ayy6 37 39 structural structural NN cord-001249-awn9ayy6 37 40 ( ( -LRB- cord-001249-awn9ayy6 37 41 Gn Gn NNP cord-001249-awn9ayy6 37 42 and and CC cord-001249-awn9ayy6 37 43 Gc Gc NNP cord-001249-awn9ayy6 37 44 ) ) -RRB- cord-001249-awn9ayy6 37 45 and and CC cord-001249-awn9ayy6 37 46 non non JJ cord-001249-awn9ayy6 37 47 - - JJ cord-001249-awn9ayy6 37 48 structural structural JJ cord-001249-awn9ayy6 37 49 glycoproteins glycoprotein NNS cord-001249-awn9ayy6 37 50 [ [ -LRB- cord-001249-awn9ayy6 37 51 37 37 CD cord-001249-awn9ayy6 37 52 , , , cord-001249-awn9ayy6 37 53 [ [ -LRB- cord-001249-awn9ayy6 37 54 47 47 CD cord-001249-awn9ayy6 37 55 ] ] -RRB- cord-001249-awn9ayy6 37 56 [ [ -LRB- cord-001249-awn9ayy6 37 57 48 48 CD cord-001249-awn9ayy6 37 58 ] ] -RRB- cord-001249-awn9ayy6 37 59 [ [ -LRB- cord-001249-awn9ayy6 37 60 49 49 CD cord-001249-awn9ayy6 37 61 ] ] -RRB- cord-001249-awn9ayy6 37 62 [ [ -LRB- cord-001249-awn9ayy6 37 63 50 50 CD cord-001249-awn9ayy6 37 64 ] ] -RRB- cord-001249-awn9ayy6 37 65 , , , cord-001249-awn9ayy6 37 66 and and CC cord-001249-awn9ayy6 37 67 the the DT cord-001249-awn9ayy6 37 68 RNA RNA NNP cord-001249-awn9ayy6 37 69 - - HYPH cord-001249-awn9ayy6 37 70 dependent dependent JJ cord-001249-awn9ayy6 37 71 RNA RNA NNP cord-001249-awn9ayy6 37 72 polymerase polymerase NN cord-001249-awn9ayy6 37 73 ( ( -LRB- cord-001249-awn9ayy6 37 74 i.e. i.e. FW cord-001249-awn9ayy6 37 75 the the DT cord-001249-awn9ayy6 37 76 L L NNP cord-001249-awn9ayy6 37 77 protein protein NN cord-001249-awn9ayy6 37 78 ) ) -RRB- cord-001249-awn9ayy6 37 79 , , , cord-001249-awn9ayy6 37 80 which which WDT cord-001249-awn9ayy6 37 81 is be VBZ cord-001249-awn9ayy6 37 82 associated associate VBN cord-001249-awn9ayy6 37 83 with with IN cord-001249-awn9ayy6 37 84 each each DT cord-001249-awn9ayy6 37 85 RNP RNP NNP cord-001249-awn9ayy6 37 86 [ [ -LRB- cord-001249-awn9ayy6 37 87 17 17 CD cord-001249-awn9ayy6 37 88 , , , cord-001249-awn9ayy6 37 89 [ [ -LRB- cord-001249-awn9ayy6 37 90 51 51 CD cord-001249-awn9ayy6 37 91 ] ] -RRB- cord-001249-awn9ayy6 37 92 [ [ -LRB- cord-001249-awn9ayy6 37 93 52 52 CD cord-001249-awn9ayy6 37 94 ] ] -RRB- cord-001249-awn9ayy6 37 95 [ [ -LRB- cord-001249-awn9ayy6 37 96 53 53 CD cord-001249-awn9ayy6 37 97 ] ] -RRB- cord-001249-awn9ayy6 37 98 . . . cord-001249-awn9ayy6 38 1 Replication replication NN cord-001249-awn9ayy6 38 2 of of IN cord-001249-awn9ayy6 38 3 nairoviruses nairoviruse NNS cord-001249-awn9ayy6 38 4 occurs occur VBZ cord-001249-awn9ayy6 38 5 in in IN cord-001249-awn9ayy6 38 6 the the DT cord-001249-awn9ayy6 38 7 cytoplasm cytoplasm NN cord-001249-awn9ayy6 38 8 , , , cord-001249-awn9ayy6 38 9 where where WRB cord-001249-awn9ayy6 38 10 the the DT cord-001249-awn9ayy6 38 11 N N NNP cord-001249-awn9ayy6 38 12 and and CC cord-001249-awn9ayy6 38 13 L L NNP cord-001249-awn9ayy6 38 14 proteins protein NNS cord-001249-awn9ayy6 38 15 are be VBP cord-001249-awn9ayy6 38 16 also also RB cord-001249-awn9ayy6 38 17 synthesised synthesise VBN cord-001249-awn9ayy6 38 18 [ [ -LRB- cord-001249-awn9ayy6 38 19 54 54 CD cord-001249-awn9ayy6 38 20 , , , cord-001249-awn9ayy6 38 21 55 55 CD cord-001249-awn9ayy6 38 22 ] ] -RRB- cord-001249-awn9ayy6 38 23 , , , cord-001249-awn9ayy6 38 24 while while IN cord-001249-awn9ayy6 38 25 the the DT cord-001249-awn9ayy6 38 26 viral viral JJ cord-001249-awn9ayy6 38 27 glycoproteins glycoprotein NNS cord-001249-awn9ayy6 38 28 are be VBP cord-001249-awn9ayy6 38 29 generated generate VBN cord-001249-awn9ayy6 38 30 on on IN cord-001249-awn9ayy6 38 31 endoplasmic endoplasmic NN cord-001249-awn9ayy6 38 32 reticulum reticulum NN cord-001249-awn9ayy6 38 33 ( ( -LRB- cord-001249-awn9ayy6 38 34 ER)associated ER)associated NNP cord-001249-awn9ayy6 38 35 ribosomes ribosome NNS cord-001249-awn9ayy6 38 36 [ [ -LRB- cord-001249-awn9ayy6 38 37 49 49 CD cord-001249-awn9ayy6 38 38 , , , cord-001249-awn9ayy6 38 39 50 50 CD cord-001249-awn9ayy6 38 40 , , , cord-001249-awn9ayy6 38 41 [ [ -LRB- cord-001249-awn9ayy6 38 42 56 56 CD cord-001249-awn9ayy6 38 43 ] ] -RRB- cord-001249-awn9ayy6 39 1 [ [ -LRB- cord-001249-awn9ayy6 39 2 57 57 CD cord-001249-awn9ayy6 39 3 ] ] -RRB- cord-001249-awn9ayy6 39 4 [ [ -LRB- cord-001249-awn9ayy6 39 5 58 58 CD cord-001249-awn9ayy6 39 6 ] ] -RRB- cord-001249-awn9ayy6 39 7 [ [ -LRB- cord-001249-awn9ayy6 39 8 59 59 CD cord-001249-awn9ayy6 39 9 ] ] -RRB- cord-001249-awn9ayy6 39 10 . . . cord-001249-awn9ayy6 40 1 Newly newly RB cord-001249-awn9ayy6 40 2 synthesised synthesise VBN cord-001249-awn9ayy6 40 3 virions virion NNS cord-001249-awn9ayy6 40 4 bud bud NN cord-001249-awn9ayy6 40 5 into into IN cord-001249-awn9ayy6 40 6 the the DT cord-001249-awn9ayy6 40 7 Golgi Golgi NNP cord-001249-awn9ayy6 40 8 and and CC cord-001249-awn9ayy6 40 9 are be VBP cord-001249-awn9ayy6 40 10 transported transport VBN cord-001249-awn9ayy6 40 11 , , , cord-001249-awn9ayy6 40 12 probably probably RB cord-001249-awn9ayy6 40 13 through through IN cord-001249-awn9ayy6 40 14 the the DT cord-001249-awn9ayy6 40 15 secretory secretory JJ cord-001249-awn9ayy6 40 16 pathway pathway NN cord-001249-awn9ayy6 40 17 , , , cord-001249-awn9ayy6 40 18 to to IN cord-001249-awn9ayy6 40 19 the the DT cord-001249-awn9ayy6 40 20 plasma plasma NN cord-001249-awn9ayy6 40 21 membrane membrane NN cord-001249-awn9ayy6 40 22 , , , cord-001249-awn9ayy6 40 23 where where WRB cord-001249-awn9ayy6 40 24 the the DT cord-001249-awn9ayy6 40 25 virions virion NNS cord-001249-awn9ayy6 40 26 exit exit VBP cord-001249-awn9ayy6 40 27 the the DT cord-001249-awn9ayy6 40 28 infected infected JJ cord-001249-awn9ayy6 40 29 cell cell NN cord-001249-awn9ayy6 40 30 after after IN cord-001249-awn9ayy6 40 31 fusion fusion NN cord-001249-awn9ayy6 40 32 of of IN cord-001249-awn9ayy6 40 33 the the DT cord-001249-awn9ayy6 40 34 carrier carrier NN cord-001249-awn9ayy6 40 35 vesicle vesicle NN cord-001249-awn9ayy6 40 36 with with IN cord-001249-awn9ayy6 40 37 the the DT cord-001249-awn9ayy6 40 38 plasma plasma NN cord-001249-awn9ayy6 40 39 membrane membrane NN cord-001249-awn9ayy6 40 40 [ [ -LRB- cord-001249-awn9ayy6 40 41 35 35 CD cord-001249-awn9ayy6 40 42 , , , cord-001249-awn9ayy6 40 43 36 36 CD cord-001249-awn9ayy6 40 44 , , , cord-001249-awn9ayy6 40 45 60 60 CD cord-001249-awn9ayy6 40 46 ] ] -RRB- cord-001249-awn9ayy6 40 47 . . . cord-001249-awn9ayy6 41 1 The the DT cord-001249-awn9ayy6 41 2 M M NNP cord-001249-awn9ayy6 41 3 segment segment NN cord-001249-awn9ayy6 41 4 mRNA mrna NN cord-001249-awn9ayy6 41 5 is be VBZ cord-001249-awn9ayy6 41 6 thought think VBN cord-001249-awn9ayy6 41 7 to to TO cord-001249-awn9ayy6 41 8 be be VB cord-001249-awn9ayy6 41 9 translated translate VBN cord-001249-awn9ayy6 41 10 as as IN cord-001249-awn9ayy6 41 11 a a DT cord-001249-awn9ayy6 41 12 single single JJ cord-001249-awn9ayy6 41 13 polyprotein polyprotein NN cord-001249-awn9ayy6 41 14 , , , cord-001249-awn9ayy6 41 15 which which WDT cord-001249-awn9ayy6 41 16 is be VBZ cord-001249-awn9ayy6 41 17 further further RB cord-001249-awn9ayy6 41 18 processed process VBN cord-001249-awn9ayy6 41 19 by by IN cord-001249-awn9ayy6 41 20 the the DT cord-001249-awn9ayy6 41 21 cellular cellular JJ cord-001249-awn9ayy6 41 22 signal signal NN cord-001249-awn9ayy6 41 23 protease protease NN cord-001249-awn9ayy6 41 24 [ [ -LRB- cord-001249-awn9ayy6 41 25 48 48 CD cord-001249-awn9ayy6 41 26 , , , cord-001249-awn9ayy6 41 27 50 50 CD cord-001249-awn9ayy6 41 28 ] ] -RRB- cord-001249-awn9ayy6 41 29 to to TO cord-001249-awn9ayy6 41 30 generate generate VB cord-001249-awn9ayy6 41 31 the the DT cord-001249-awn9ayy6 41 32 glycoproteins glycoprotein NNS cord-001249-awn9ayy6 41 33 PreGn pregn PRP cord-001249-awn9ayy6 41 34 and and CC cord-001249-awn9ayy6 41 35 PreGc PreGc NNPS cord-001249-awn9ayy6 41 36 ; ; : cord-001249-awn9ayy6 41 37 in in IN cord-001249-awn9ayy6 41 38 CCHFV CCHFV NNP cord-001249-awn9ayy6 41 39 these these DT cord-001249-awn9ayy6 41 40 precursors precursor NNS cord-001249-awn9ayy6 41 41 are be VBP cord-001249-awn9ayy6 41 42 further further RB cord-001249-awn9ayy6 41 43 processed process VBN cord-001249-awn9ayy6 41 44 into into IN cord-001249-awn9ayy6 41 45 mature mature JJ cord-001249-awn9ayy6 41 46 glycoproteins glycoprotein NNS cord-001249-awn9ayy6 41 47 Gn Gn NNP cord-001249-awn9ayy6 41 48 and and CC cord-001249-awn9ayy6 41 49 Gc Gc NNP cord-001249-awn9ayy6 41 50 in in IN cord-001249-awn9ayy6 41 51 a a DT cord-001249-awn9ayy6 41 52 process process NN cord-001249-awn9ayy6 41 53 which which WDT cord-001249-awn9ayy6 41 54 employs employ VBZ cord-001249-awn9ayy6 41 55 other other JJ cord-001249-awn9ayy6 41 56 cellular cellular JJ cord-001249-awn9ayy6 41 57 proteases protease NNS cord-001249-awn9ayy6 41 58 [ [ -LRB- cord-001249-awn9ayy6 41 59 50 50 CD cord-001249-awn9ayy6 41 60 , , , cord-001249-awn9ayy6 41 61 [ [ -LRB- cord-001249-awn9ayy6 41 62 57 57 CD cord-001249-awn9ayy6 41 63 ] ] -RRB- cord-001249-awn9ayy6 41 64 [ [ -LRB- cord-001249-awn9ayy6 41 65 58 58 CD cord-001249-awn9ayy6 41 66 ] ] -RRB- cord-001249-awn9ayy6 41 67 [ [ -LRB- cord-001249-awn9ayy6 41 68 59 59 CD cord-001249-awn9ayy6 41 69 ] ] -RRB- cord-001249-awn9ayy6 41 70 . . . cord-001249-awn9ayy6 42 1 Since since IN cord-001249-awn9ayy6 42 2 the the DT cord-001249-awn9ayy6 42 3 glycoproteins glycoprotein NNS cord-001249-awn9ayy6 42 4 of of IN cord-001249-awn9ayy6 42 5 nairoviruses nairoviruse NNS cord-001249-awn9ayy6 42 6 mature mature VB cord-001249-awn9ayy6 42 7 in in IN cord-001249-awn9ayy6 42 8 the the DT cord-001249-awn9ayy6 42 9 ER ER NNP cord-001249-awn9ayy6 42 10 and and CC cord-001249-awn9ayy6 42 11 Golgi Golgi NNP cord-001249-awn9ayy6 42 12 , , , cord-001249-awn9ayy6 42 13 and and CC cord-001249-awn9ayy6 42 14 newly newly RB cord-001249-awn9ayy6 42 15 generated generate VBN cord-001249-awn9ayy6 42 16 virions virion NNS cord-001249-awn9ayy6 42 17 bud bud NN cord-001249-awn9ayy6 42 18 in in IN cord-001249-awn9ayy6 42 19 the the DT cord-001249-awn9ayy6 42 20 Golgi Golgi NNP cord-001249-awn9ayy6 42 21 , , , cord-001249-awn9ayy6 42 22 nairoviruses nairoviruse NNS cord-001249-awn9ayy6 42 23 would would MD cord-001249-awn9ayy6 42 24 be be VB cord-001249-awn9ayy6 42 25 expected expect VBN cord-001249-awn9ayy6 42 26 to to TO cord-001249-awn9ayy6 42 27 affect affect VB cord-001249-awn9ayy6 42 28 the the DT cord-001249-awn9ayy6 42 29 secretory secretory JJ cord-001249-awn9ayy6 42 30 pathway pathway NN cord-001249-awn9ayy6 42 31 . . . cord-001249-awn9ayy6 43 1 However however RB cord-001249-awn9ayy6 43 2 the the DT cord-001249-awn9ayy6 43 3 effect effect NN cord-001249-awn9ayy6 43 4 of of IN cord-001249-awn9ayy6 43 5 nairovirus nairovirus JJ cord-001249-awn9ayy6 43 6 replication replication NN cord-001249-awn9ayy6 43 7 on on IN cord-001249-awn9ayy6 43 8 specific specific JJ cord-001249-awn9ayy6 43 9 cellular cellular JJ cord-001249-awn9ayy6 43 10 compartments compartment NNS cord-001249-awn9ayy6 43 11 has have VBZ cord-001249-awn9ayy6 43 12 not not RB cord-001249-awn9ayy6 43 13 been be VBN cord-001249-awn9ayy6 43 14 studied study VBN cord-001249-awn9ayy6 43 15 ; ; : cord-001249-awn9ayy6 43 16 in in IN cord-001249-awn9ayy6 43 17 this this DT cord-001249-awn9ayy6 43 18 study study NN cord-001249-awn9ayy6 43 19 we -PRON- PRP cord-001249-awn9ayy6 43 20 used use VBD cord-001249-awn9ayy6 43 21 specific specific JJ cord-001249-awn9ayy6 43 22 antibodies antibody NNS cord-001249-awn9ayy6 43 23 in in IN cord-001249-awn9ayy6 43 24 combination combination NN cord-001249-awn9ayy6 43 25 with with IN cord-001249-awn9ayy6 43 26 laser laser NN cord-001249-awn9ayy6 43 27 scanning scanning NN cord-001249-awn9ayy6 43 28 confocal confocal JJ cord-001249-awn9ayy6 43 29 microscopy microscopy NNS cord-001249-awn9ayy6 43 30 to to TO cord-001249-awn9ayy6 43 31 study study VB cord-001249-awn9ayy6 43 32 the the DT cord-001249-awn9ayy6 43 33 effects effect NNS cord-001249-awn9ayy6 43 34 of of IN cord-001249-awn9ayy6 43 35 NSDV NSDV NNP cord-001249-awn9ayy6 43 36 on on IN cord-001249-awn9ayy6 43 37 the the DT cord-001249-awn9ayy6 43 38 secretory secretory JJ cord-001249-awn9ayy6 43 39 pathway pathway NN cord-001249-awn9ayy6 43 40 of of IN cord-001249-awn9ayy6 43 41 infected infected JJ cord-001249-awn9ayy6 43 42 cells cell NNS cord-001249-awn9ayy6 43 43 . . . cord-001249-awn9ayy6 44 1 We -PRON- PRP cord-001249-awn9ayy6 44 2 observed observe VBD cord-001249-awn9ayy6 44 3 that that IN cord-001249-awn9ayy6 44 4 while while IN cord-001249-awn9ayy6 44 5 NSDV NSDV NNP cord-001249-awn9ayy6 44 6 replication replication NN cord-001249-awn9ayy6 44 7 has have VBZ cord-001249-awn9ayy6 44 8 no no DT cord-001249-awn9ayy6 44 9 obvious obvious JJ cord-001249-awn9ayy6 44 10 effect effect NN cord-001249-awn9ayy6 44 11 on on IN cord-001249-awn9ayy6 44 12 the the DT cord-001249-awn9ayy6 44 13 structure structure NN cord-001249-awn9ayy6 44 14 of of IN cord-001249-awn9ayy6 44 15 the the DT cord-001249-awn9ayy6 44 16 ER ER NNP cord-001249-awn9ayy6 44 17 , , , cord-001249-awn9ayy6 44 18 the the DT cord-001249-awn9ayy6 44 19 ER ER NNP cord-001249-awn9ayy6 44 20 - - HYPH cord-001249-awn9ayy6 44 21 Golgi Golgi NNP cord-001249-awn9ayy6 44 22 intermediate intermediate JJ cord-001249-awn9ayy6 44 23 compartment compartment NN cord-001249-awn9ayy6 44 24 ( ( -LRB- cord-001249-awn9ayy6 44 25 ERGIC ERGIC NNP cord-001249-awn9ayy6 44 26 ) ) -RRB- cord-001249-awn9ayy6 44 27 or or CC cord-001249-awn9ayy6 44 28 the the DT cord-001249-awn9ayy6 44 29 Golgi Golgi NNP cord-001249-awn9ayy6 44 30 , , , cord-001249-awn9ayy6 44 31 it -PRON- PRP cord-001249-awn9ayy6 44 32 induces induce VBZ cord-001249-awn9ayy6 44 33 redistribution redistribution NN cord-001249-awn9ayy6 44 34 of of IN cord-001249-awn9ayy6 44 35 luminal luminal JJ cord-001249-awn9ayy6 44 36 ER er NN cord-001249-awn9ayy6 44 37 oxidoreductases oxidoreductase NNS cord-001249-awn9ayy6 44 38 protein protein NN cord-001249-awn9ayy6 44 39 disulphide disulphide NN cord-001249-awn9ayy6 44 40 isomerase isomerase NN cord-001249-awn9ayy6 44 41 ( ( -LRB- cord-001249-awn9ayy6 44 42 PDI PDI NNP cord-001249-awn9ayy6 44 43 ) ) -RRB- cord-001249-awn9ayy6 44 44 and and CC cord-001249-awn9ayy6 44 45 ERp57 ERp57 NNS cord-001249-awn9ayy6 44 46 , , , cord-001249-awn9ayy6 44 47 proteins protein NNS cord-001249-awn9ayy6 44 48 which which WDT cord-001249-awn9ayy6 44 49 are be VBP cord-001249-awn9ayy6 44 50 involved involve VBN cord-001249-awn9ayy6 44 51 in in IN cord-001249-awn9ayy6 44 52 the the DT cord-001249-awn9ayy6 44 53 formation formation NN cord-001249-awn9ayy6 44 54 and and CC cord-001249-awn9ayy6 44 55 rearrangement rearrangement NN cord-001249-awn9ayy6 44 56 of of IN cord-001249-awn9ayy6 44 57 disulphide disulphide JJ cord-001249-awn9ayy6 44 58 bonds bond NNS cord-001249-awn9ayy6 44 59 during during IN cord-001249-awn9ayy6 44 60 protein protein NN cord-001249-awn9ayy6 44 61 folding folding NN cord-001249-awn9ayy6 44 62 . . . cord-001249-awn9ayy6 45 1 In in IN cord-001249-awn9ayy6 45 2 NSDV NSDV NNP cord-001249-awn9ayy6 45 3 - - HYPH cord-001249-awn9ayy6 45 4 infected infect VBN cord-001249-awn9ayy6 45 5 cells cell NNS cord-001249-awn9ayy6 45 6 , , , cord-001249-awn9ayy6 45 7 both both CC cord-001249-awn9ayy6 45 8 PDI PDI NNP cord-001249-awn9ayy6 45 9 and and CC cord-001249-awn9ayy6 45 10 ERp57 ERp57 NNS cord-001249-awn9ayy6 45 11 are be VBP cord-001249-awn9ayy6 45 12 translocated translocate VBN cord-001249-awn9ayy6 45 13 from from IN cord-001249-awn9ayy6 45 14 the the DT cord-001249-awn9ayy6 45 15 ER ER NNP cord-001249-awn9ayy6 45 16 to to IN cord-001249-awn9ayy6 45 17 the the DT cord-001249-awn9ayy6 45 18 cell cell NN cord-001249-awn9ayy6 45 19 surface surface NN cord-001249-awn9ayy6 45 20 and and CC cord-001249-awn9ayy6 45 21 to to IN cord-001249-awn9ayy6 45 22 the the DT cord-001249-awn9ayy6 45 23 medium medium NN cord-001249-awn9ayy6 45 24 surrounding surround VBG cord-001249-awn9ayy6 45 25 the the DT cord-001249-awn9ayy6 45 26 infected infected JJ cord-001249-awn9ayy6 45 27 cells cell NNS cord-001249-awn9ayy6 45 28 , , , cord-001249-awn9ayy6 45 29 a a DT cord-001249-awn9ayy6 45 30 process process NN cord-001249-awn9ayy6 45 31 which which WDT cord-001249-awn9ayy6 45 32 seems seem VBZ cord-001249-awn9ayy6 45 33 to to TO cord-001249-awn9ayy6 45 34 depend depend VB cord-001249-awn9ayy6 45 35 on on IN cord-001249-awn9ayy6 45 36 the the DT cord-001249-awn9ayy6 45 37 NSDV NSDV NNP cord-001249-awn9ayy6 45 38 glycoprotein glycoprotein NN cord-001249-awn9ayy6 45 39 precursor precursor NN cord-001249-awn9ayy6 45 40 PreGn pregn NN cord-001249-awn9ayy6 45 41 . . . cord-001249-awn9ayy6 46 1 All all DT cord-001249-awn9ayy6 46 2 cell cell NN cord-001249-awn9ayy6 46 3 culture culture NN cord-001249-awn9ayy6 46 4 was be VBD cord-001249-awn9ayy6 46 5 carried carry VBN cord-001249-awn9ayy6 46 6 out out RP cord-001249-awn9ayy6 46 7 as as IN cord-001249-awn9ayy6 46 8 previously previously RB cord-001249-awn9ayy6 46 9 described describe VBN cord-001249-awn9ayy6 46 10 [ [ -LRB- cord-001249-awn9ayy6 46 11 29 29 CD cord-001249-awn9ayy6 46 12 ] ] -RRB- cord-001249-awn9ayy6 46 13 , , , cord-001249-awn9ayy6 46 14 with with IN cord-001249-awn9ayy6 46 15 the the DT cord-001249-awn9ayy6 46 16 exception exception NN cord-001249-awn9ayy6 46 17 of of IN cord-001249-awn9ayy6 46 18 CJE102 cje102 JJ cord-001249-awn9ayy6 46 19 cells cell NNS cord-001249-awn9ayy6 46 20 . . . cord-001249-awn9ayy6 47 1 These these DT cord-001249-awn9ayy6 47 2 cells cell NNS cord-001249-awn9ayy6 47 3 are be VBP cord-001249-awn9ayy6 47 4 a a DT cord-001249-awn9ayy6 47 5 caprine caprine NN cord-001249-awn9ayy6 47 6 endothelial endothelial JJ cord-001249-awn9ayy6 47 7 cell cell NN cord-001249-awn9ayy6 47 8 line line NN cord-001249-awn9ayy6 47 9 derived derive VBN cord-001249-awn9ayy6 47 10 in in IN cord-001249-awn9ayy6 47 11 1999 1999 CD cord-001249-awn9ayy6 47 12 from from IN cord-001249-awn9ayy6 47 13 circulating circulate VBG cord-001249-awn9ayy6 47 14 endothelial endothelial JJ cord-001249-awn9ayy6 47 15 cells cell NNS cord-001249-awn9ayy6 47 16 isolated isolate VBN cord-001249-awn9ayy6 47 17 from from IN cord-001249-awn9ayy6 47 18 a a DT cord-001249-awn9ayy6 47 19 naïve naïve JJ cord-001249-awn9ayy6 47 20 Creole Creole NNP cord-001249-awn9ayy6 47 21 goat goat NN cord-001249-awn9ayy6 47 22 that that WDT cord-001249-awn9ayy6 47 23 was be VBD cord-001249-awn9ayy6 47 24 part part NN cord-001249-awn9ayy6 47 25 of of IN cord-001249-awn9ayy6 47 26 a a DT cord-001249-awn9ayy6 47 27 herd herd NN cord-001249-awn9ayy6 47 28 kept keep VBN cord-001249-awn9ayy6 47 29 for for IN cord-001249-awn9ayy6 47 30 research research NN cord-001249-awn9ayy6 47 31 purposes purpose NNS cord-001249-awn9ayy6 47 32 by by IN cord-001249-awn9ayy6 47 33 the the DT cord-001249-awn9ayy6 47 34 French French NNP cord-001249-awn9ayy6 47 35 Centre Centre NNP cord-001249-awn9ayy6 47 36 for for IN cord-001249-awn9ayy6 47 37 Agricultural Agricultural NNP cord-001249-awn9ayy6 47 38 Research Research NNP cord-001249-awn9ayy6 47 39 for for IN cord-001249-awn9ayy6 47 40 Development Development NNP cord-001249-awn9ayy6 47 41 ( ( -LRB- cord-001249-awn9ayy6 47 42 CIRAD CIRAD NNP cord-001249-awn9ayy6 47 43 ) ) -RRB- cord-001249-awn9ayy6 47 44 . . . cord-001249-awn9ayy6 48 1 The the DT cord-001249-awn9ayy6 48 2 blood blood NN cord-001249-awn9ayy6 48 3 was be VBD cord-001249-awn9ayy6 48 4 taken take VBN cord-001249-awn9ayy6 48 5 for for IN cord-001249-awn9ayy6 48 6 the the DT cord-001249-awn9ayy6 48 7 purpose purpose NN cord-001249-awn9ayy6 48 8 of of IN cord-001249-awn9ayy6 48 9 isolating isolate VBG cord-001249-awn9ayy6 48 10 the the DT cord-001249-awn9ayy6 48 11 cells cell NNS cord-001249-awn9ayy6 48 12 . . . cord-001249-awn9ayy6 49 1 All all DT cord-001249-awn9ayy6 49 2 animal animal NN cord-001249-awn9ayy6 49 3 sampling sampling NN cord-001249-awn9ayy6 49 4 was be VBD cord-001249-awn9ayy6 49 5 conducted conduct VBN cord-001249-awn9ayy6 49 6 according accord VBG cord-001249-awn9ayy6 49 7 to to IN cord-001249-awn9ayy6 49 8 internationally internationally RB cord-001249-awn9ayy6 49 9 approved approve VBN cord-001249-awn9ayy6 49 10 OIE OIE NNP cord-001249-awn9ayy6 49 11 standards standard NNS cord-001249-awn9ayy6 49 12 , , , cord-001249-awn9ayy6 49 13 under under IN cord-001249-awn9ayy6 49 14 personal personal JJ cord-001249-awn9ayy6 49 15 and and CC cord-001249-awn9ayy6 49 16 institutional institutional JJ cord-001249-awn9ayy6 49 17 authorizations authorization NNS cord-001249-awn9ayy6 49 18 set set VBN cord-001249-awn9ayy6 49 19 forth forth RP cord-001249-awn9ayy6 49 20 by by IN cord-001249-awn9ayy6 49 21 the the DT cord-001249-awn9ayy6 49 22 director director NN cord-001249-awn9ayy6 49 23 of of IN cord-001249-awn9ayy6 49 24 the the DT cord-001249-awn9ayy6 49 25 veterinary veterinary JJ cord-001249-awn9ayy6 49 26 services service NNS cord-001249-awn9ayy6 49 27 of of IN cord-001249-awn9ayy6 49 28 Guadeloupe Guadeloupe NNP cord-001249-awn9ayy6 49 29 on on IN cord-001249-awn9ayy6 49 30 behalf behalf NN cord-001249-awn9ayy6 49 31 of of IN cord-001249-awn9ayy6 49 32 the the DT cord-001249-awn9ayy6 49 33 Prefect Prefect NNP cord-001249-awn9ayy6 49 34 of of IN cord-001249-awn9ayy6 49 35 Guadeloupe Guadeloupe NNP cord-001249-awn9ayy6 49 36 ( ( -LRB- cord-001249-awn9ayy6 49 37 authorization authorization NN cord-001249-awn9ayy6 49 38 number number NN cord-001249-awn9ayy6 49 39 : : : cord-001249-awn9ayy6 49 40 A-971 a-971 JJ cord-001249-awn9ayy6 49 41 - - HYPH cord-001249-awn9ayy6 49 42 18 18 CD cord-001249-awn9ayy6 49 43 - - HYPH cord-001249-awn9ayy6 49 44 01 01 CD cord-001249-awn9ayy6 49 45 ) ) -RRB- cord-001249-awn9ayy6 49 46 and and CC cord-001249-awn9ayy6 49 47 according accord VBG cord-001249-awn9ayy6 49 48 to to IN cord-001249-awn9ayy6 49 49 the the DT cord-001249-awn9ayy6 49 50 regulations regulation NNS cord-001249-awn9ayy6 49 51 in in IN cord-001249-awn9ayy6 49 52 the the DT cord-001249-awn9ayy6 49 53 Guide Guide NNP cord-001249-awn9ayy6 49 54 to to IN cord-001249-awn9ayy6 49 55 the the DT cord-001249-awn9ayy6 49 56 Care Care NNP cord-001249-awn9ayy6 49 57 and and CC cord-001249-awn9ayy6 49 58 Use Use NNP cord-001249-awn9ayy6 49 59 of of IN cord-001249-awn9ayy6 49 60 Experimental Experimental NNP cord-001249-awn9ayy6 49 61 Animals Animals NNPS cord-001249-awn9ayy6 49 62 provided provide VBN cord-001249-awn9ayy6 49 63 by by IN cord-001249-awn9ayy6 49 64 the the DT cord-001249-awn9ayy6 49 65 French French NNP cord-001249-awn9ayy6 49 66 Ministry Ministry NNP cord-001249-awn9ayy6 49 67 of of IN cord-001249-awn9ayy6 49 68 Agriculture Agriculture NNP cord-001249-awn9ayy6 49 69 , , , cord-001249-awn9ayy6 49 70 which which WDT cord-001249-awn9ayy6 49 71 follow follow VBP cord-001249-awn9ayy6 49 72 the the DT cord-001249-awn9ayy6 49 73 relevant relevant JJ cord-001249-awn9ayy6 49 74 EU EU NNP cord-001249-awn9ayy6 49 75 directive directive NN cord-001249-awn9ayy6 49 76 [ [ -LRB- cord-001249-awn9ayy6 49 77 61 61 CD cord-001249-awn9ayy6 49 78 ] ] -RRB- cord-001249-awn9ayy6 49 79 . . . cord-001249-awn9ayy6 50 1 Cell cell NN cord-001249-awn9ayy6 50 2 line line NN cord-001249-awn9ayy6 50 3 CJE102 CJE102 NNPS cord-001249-awn9ayy6 50 4 was be VBD cord-001249-awn9ayy6 50 5 the the DT cord-001249-awn9ayy6 50 6 kind kind JJ cord-001249-awn9ayy6 50 7 gift gift NN cord-001249-awn9ayy6 50 8 of of IN cord-001249-awn9ayy6 50 9 Dr Dr NNP cord-001249-awn9ayy6 50 10 Nathalie Nathalie NNP cord-001249-awn9ayy6 50 11 Vachiéry Vachiéry NNP cord-001249-awn9ayy6 50 12 , , , cord-001249-awn9ayy6 50 13 Département Département NNP cord-001249-awn9ayy6 50 14 EMVT EMVT NNP cord-001249-awn9ayy6 50 15 du du NNP cord-001249-awn9ayy6 50 16 CIRAD CIRAD NNP cord-001249-awn9ayy6 50 17 , , , cord-001249-awn9ayy6 50 18 Domaine Domaine NNP cord-001249-awn9ayy6 50 19 de de NNP cord-001249-awn9ayy6 50 20 Duclos Duclos NNP cord-001249-awn9ayy6 50 21 , , , cord-001249-awn9ayy6 50 22 Prise Prise NNP cord-001249-awn9ayy6 50 23 d'Eau d'Eau NNP cord-001249-awn9ayy6 50 24 , , , cord-001249-awn9ayy6 50 25 97170 97170 CD cord-001249-awn9ayy6 50 26 Petit Petit NNP cord-001249-awn9ayy6 50 27 Bourg Bourg NNP cord-001249-awn9ayy6 50 28 , , , cord-001249-awn9ayy6 50 29 Guadeloupe Guadeloupe NNP cord-001249-awn9ayy6 50 30 . . . cord-001249-awn9ayy6 51 1 The the DT cord-001249-awn9ayy6 51 2 cells cell NNS cord-001249-awn9ayy6 51 3 were be VBD cord-001249-awn9ayy6 51 4 cultured cultured JJ cord-001249-awn9ayy6 51 5 in in IN cord-001249-awn9ayy6 51 6 Glasgow Glasgow NNP cord-001249-awn9ayy6 51 7 Modified Modified NNP cord-001249-awn9ayy6 51 8 Eagle Eagle NNP cord-001249-awn9ayy6 51 9 's 's POS cord-001249-awn9ayy6 51 10 Medium medium NN cord-001249-awn9ayy6 51 11 ( ( -LRB- cord-001249-awn9ayy6 51 12 GMEM GMEM NNP cord-001249-awn9ayy6 51 13 ) ) -RRB- cord-001249-awn9ayy6 51 14 containing contain VBG cord-001249-awn9ayy6 51 15 10 10 CD cord-001249-awn9ayy6 51 16 % % NN cord-001249-awn9ayy6 51 17 foetal foetal JJ cord-001249-awn9ayy6 51 18 calf calf NN cord-001249-awn9ayy6 51 19 serum serum NN cord-001249-awn9ayy6 51 20 ( ( -LRB- cord-001249-awn9ayy6 51 21 FCS FCS NNP cord-001249-awn9ayy6 51 22 ) ) -RRB- cord-001249-awn9ayy6 51 23 , , , cord-001249-awn9ayy6 51 24 penicillin penicillin NN cord-001249-awn9ayy6 51 25 ( ( -LRB- cord-001249-awn9ayy6 51 26 100 100 CD cord-001249-awn9ayy6 51 27 U U NNP cord-001249-awn9ayy6 51 28 / / SYM cord-001249-awn9ayy6 51 29 ml ml NN cord-001249-awn9ayy6 51 30 ) ) -RRB- cord-001249-awn9ayy6 51 31 , , . cord-001249-awn9ayy6 52 1 streptomycin streptomycin NN cord-001249-awn9ayy6 52 2 sulphate sulphate NN cord-001249-awn9ayy6 52 3 ( ( -LRB- cord-001249-awn9ayy6 52 4 100 100 CD cord-001249-awn9ayy6 52 5 mg mg NNP cord-001249-awn9ayy6 52 6 / / SYM cord-001249-awn9ayy6 52 7 ml ml NNP cord-001249-awn9ayy6 52 8 ) ) -RRB- cord-001249-awn9ayy6 52 9 , , , cord-001249-awn9ayy6 52 10 2 2 CD cord-001249-awn9ayy6 52 11 mM mm CD cord-001249-awn9ayy6 52 12 L l NN cord-001249-awn9ayy6 52 13 - - HYPH cord-001249-awn9ayy6 52 14 glutamine glutamine NN cord-001249-awn9ayy6 52 15 and and CC cord-001249-awn9ayy6 52 16 5 5 CD cord-001249-awn9ayy6 52 17 % % NN cord-001249-awn9ayy6 52 18 tryptose tryptose JJ cord-001249-awn9ayy6 52 19 phosphate phosphate NN cord-001249-awn9ayy6 52 20 broth broth NN cord-001249-awn9ayy6 52 21 . . . cord-001249-awn9ayy6 53 1 The the DT cord-001249-awn9ayy6 53 2 highly highly JJ cord-001249-awn9ayy6 53 3 tissue tissue NN cord-001249-awn9ayy6 53 4 culture culture NN cord-001249-awn9ayy6 53 5 passaged passage VBD cord-001249-awn9ayy6 53 6 NSDV NSDV NNP cord-001249-awn9ayy6 53 7 isolate isolate VBP cord-001249-awn9ayy6 53 8 from from IN cord-001249-awn9ayy6 53 9 Uganda Uganda NNP cord-001249-awn9ayy6 53 10 ( ( -LRB- cord-001249-awn9ayy6 53 11 NSDVu NSDVu NNP cord-001249-awn9ayy6 53 12 ) ) -RRB- cord-001249-awn9ayy6 53 13 ( ( -LRB- cord-001249-awn9ayy6 53 14 ND66-PC9 ND66-PC9 NNP cord-001249-awn9ayy6 53 15 ) ) -RRB- cord-001249-awn9ayy6 53 16 , , , cord-001249-awn9ayy6 53 17 which which WDT cord-001249-awn9ayy6 53 18 was be VBD cord-001249-awn9ayy6 53 19 obtained obtain VBN cord-001249-awn9ayy6 53 20 from from IN cord-001249-awn9ayy6 53 21 Dr Dr NNP cord-001249-awn9ayy6 53 22 Piet Piet NNP cord-001249-awn9ayy6 53 23 van van NNP cord-001249-awn9ayy6 53 24 Rijn Rijn NNP cord-001249-awn9ayy6 53 25 , , , cord-001249-awn9ayy6 53 26 Central Central NNP cord-001249-awn9ayy6 53 27 Veterinary Veterinary NNP cord-001249-awn9ayy6 53 28 Institute Institute NNP cord-001249-awn9ayy6 53 29 of of IN cord-001249-awn9ayy6 53 30 Wageningen Wageningen NNP cord-001249-awn9ayy6 53 31 , , , cord-001249-awn9ayy6 53 32 Netherlands Netherlands NNP cord-001249-awn9ayy6 53 33 , , , cord-001249-awn9ayy6 53 34 at at IN cord-001249-awn9ayy6 53 35 the the DT cord-001249-awn9ayy6 53 36 75 75 CD cord-001249-awn9ayy6 53 37 th th XX cord-001249-awn9ayy6 53 38 tissue tissue NN cord-001249-awn9ayy6 53 39 culture culture NN cord-001249-awn9ayy6 53 40 passage passage NN cord-001249-awn9ayy6 53 41 , , , cord-001249-awn9ayy6 53 42 and and CC cord-001249-awn9ayy6 53 43 the the DT cord-001249-awn9ayy6 53 44 pathogenic pathogenic JJ cord-001249-awn9ayy6 53 45 GV GV NNP cord-001249-awn9ayy6 53 46 isolate isolate VB cord-001249-awn9ayy6 53 47 ( ( -LRB- cord-001249-awn9ayy6 53 48 NSDVi NSDVi NNS cord-001249-awn9ayy6 53 49 ) ) -RRB- cord-001249-awn9ayy6 54 1 ( ( -LRB- cord-001249-awn9ayy6 54 2 IG619 IG619 NNP cord-001249-awn9ayy6 54 3 , , , cord-001249-awn9ayy6 54 4 TVPII TVPII NNP cord-001249-awn9ayy6 54 5 236 236 CD cord-001249-awn9ayy6 54 6 ) ) -RRB- cord-001249-awn9ayy6 54 7 , , , cord-001249-awn9ayy6 54 8 which which WDT cord-001249-awn9ayy6 54 9 was be VBD cord-001249-awn9ayy6 54 10 obtained obtain VBN cord-001249-awn9ayy6 54 11 from from IN cord-001249-awn9ayy6 54 12 Professor Professor NNP cord-001249-awn9ayy6 54 13 Robert Robert NNP cord-001249-awn9ayy6 54 14 B. B. NNP cord-001249-awn9ayy6 54 15 Tesh Tesh NNP cord-001249-awn9ayy6 54 16 , , , cord-001249-awn9ayy6 54 17 The the DT cord-001249-awn9ayy6 54 18 World World NNP cord-001249-awn9ayy6 54 19 Reference Reference NNP cord-001249-awn9ayy6 54 20 Center Center NNP cord-001249-awn9ayy6 54 21 for for IN cord-001249-awn9ayy6 54 22 Emerging Emerging NNP cord-001249-awn9ayy6 54 23 Viruses Viruses NNPS cord-001249-awn9ayy6 54 24 and and CC cord-001249-awn9ayy6 54 25 Arboviruses Arboviruses NNP cord-001249-awn9ayy6 54 26 at at IN cord-001249-awn9ayy6 54 27 the the DT cord-001249-awn9ayy6 54 28 Galveston Galveston NNP cord-001249-awn9ayy6 54 29 National National NNP cord-001249-awn9ayy6 54 30 Laboratory Laboratory NNP cord-001249-awn9ayy6 54 31 , , , cord-001249-awn9ayy6 54 32 University University NNP cord-001249-awn9ayy6 54 33 of of IN cord-001249-awn9ayy6 54 34 Texas Texas NNP cord-001249-awn9ayy6 54 35 Medical Medical NNP cord-001249-awn9ayy6 54 36 Branch Branch NNP cord-001249-awn9ayy6 54 37 , , , cord-001249-awn9ayy6 54 38 Galveston Galveston NNP cord-001249-awn9ayy6 54 39 , , , cord-001249-awn9ayy6 54 40 Texas Texas NNP cord-001249-awn9ayy6 54 41 , , , cord-001249-awn9ayy6 54 42 USA USA NNP cord-001249-awn9ayy6 54 43 , , , cord-001249-awn9ayy6 54 44 were be VBD cord-001249-awn9ayy6 54 45 also also RB cord-001249-awn9ayy6 54 46 already already RB cord-001249-awn9ayy6 54 47 described describe VBN cord-001249-awn9ayy6 54 48 by by IN cord-001249-awn9ayy6 54 49 us -PRON- PRP cord-001249-awn9ayy6 54 50 [ [ -LRB- cord-001249-awn9ayy6 54 51 29 29 CD cord-001249-awn9ayy6 54 52 ] ] -RRB- cord-001249-awn9ayy6 54 53 . . . cord-001249-awn9ayy6 55 1 All all DT cord-001249-awn9ayy6 55 2 DNA dna NN cord-001249-awn9ayy6 55 3 manipulation manipulation NN cord-001249-awn9ayy6 55 4 was be VBD cord-001249-awn9ayy6 55 5 done do VBN cord-001249-awn9ayy6 55 6 using use VBG cord-001249-awn9ayy6 55 7 standard standard JJ cord-001249-awn9ayy6 55 8 methods method NNS cord-001249-awn9ayy6 55 9 ; ; , cord-001249-awn9ayy6 55 10 plasmids plasmid NNS cord-001249-awn9ayy6 55 11 were be VBD cord-001249-awn9ayy6 55 12 grown grow VBN cord-001249-awn9ayy6 55 13 in in IN cord-001249-awn9ayy6 55 14 Escherichia Escherichia NNP cord-001249-awn9ayy6 55 15 coli coli VBZ cord-001249-awn9ayy6 55 16 DH5a DH5a NNP cord-001249-awn9ayy6 55 17 and and CC cord-001249-awn9ayy6 55 18 DNA DNA NNP cord-001249-awn9ayy6 55 19 was be VBD cord-001249-awn9ayy6 55 20 purified purify VBN cord-001249-awn9ayy6 55 21 using use VBG cord-001249-awn9ayy6 55 22 CsCl CsCl NNP cord-001249-awn9ayy6 55 23 gradients gradient NNS cord-001249-awn9ayy6 55 24 . . . cord-001249-awn9ayy6 56 1 Plasmids Plasmids NNP cord-001249-awn9ayy6 56 2 pcDNA6-GV pcDNA6-GV NNP cord-001249-awn9ayy6 56 3 - - HYPH cord-001249-awn9ayy6 56 4 N N NNP cord-001249-awn9ayy6 56 5 and and CC cord-001249-awn9ayy6 56 6 pcDNA6-GV pcDNA6-GV NNP cord-001249-awn9ayy6 56 7 - - HYPH cord-001249-awn9ayy6 56 8 La La NNP cord-001249-awn9ayy6 56 9 plasmids plasmid NNS cord-001249-awn9ayy6 56 10 have have VBP cord-001249-awn9ayy6 56 11 been be VBN cord-001249-awn9ayy6 56 12 previously previously RB cord-001249-awn9ayy6 56 13 described describe VBN cord-001249-awn9ayy6 56 14 [ [ -LRB- cord-001249-awn9ayy6 56 15 61 61 CD cord-001249-awn9ayy6 56 16 ] ] -RRB- cord-001249-awn9ayy6 56 17 . . . cord-001249-awn9ayy6 57 1 Plasmid Plasmid NNP cord-001249-awn9ayy6 57 2 pCAGGS pCAGGS NNP cord-001249-awn9ayy6 57 3 - - HYPH cord-001249-awn9ayy6 57 4 MCSII MCSII NNP cord-001249-awn9ayy6 57 5 was be VBD cord-001249-awn9ayy6 57 6 the the DT cord-001249-awn9ayy6 57 7 gift gift NN cord-001249-awn9ayy6 57 8 of of IN cord-001249-awn9ayy6 57 9 Professor Professor NNP cord-001249-awn9ayy6 57 10 Adolfo Adolfo NNP cord-001249-awn9ayy6 57 11 Garcia Garcia NNP cord-001249-awn9ayy6 57 12 - - HYPH cord-001249-awn9ayy6 57 13 Sastre Sastre NNP cord-001249-awn9ayy6 57 14 , , , cord-001249-awn9ayy6 57 15 Mount Mount NNP cord-001249-awn9ayy6 57 16 Sinai Sinai NNP cord-001249-awn9ayy6 57 17 School School NNP cord-001249-awn9ayy6 57 18 of of IN cord-001249-awn9ayy6 57 19 Medicine Medicine NNP cord-001249-awn9ayy6 57 20 , , , cord-001249-awn9ayy6 57 21 New New NNP cord-001249-awn9ayy6 57 22 York York NNP cord-001249-awn9ayy6 57 23 , , , cord-001249-awn9ayy6 57 24 USA USA NNP cord-001249-awn9ayy6 57 25 . . . cord-001249-awn9ayy6 58 1 The the DT cord-001249-awn9ayy6 58 2 pCAGGs_MCSII_V5 pcaggs_mcsii_v5 NN cord-001249-awn9ayy6 58 3 construct construct NN cord-001249-awn9ayy6 58 4 was be VBD cord-001249-awn9ayy6 58 5 generated generate VBN cord-001249-awn9ayy6 58 6 by by IN cord-001249-awn9ayy6 58 7 insertion insertion NN cord-001249-awn9ayy6 58 8 of of IN cord-001249-awn9ayy6 58 9 V5 v5 NN cord-001249-awn9ayy6 58 10 tag tag NN cord-001249-awn9ayy6 58 11 sequence sequence NN cord-001249-awn9ayy6 58 12 into into IN cord-001249-awn9ayy6 58 13 the the DT cord-001249-awn9ayy6 58 14 multiple multiple JJ cord-001249-awn9ayy6 58 15 cloning cloning NN cord-001249-awn9ayy6 58 16 site site NN cord-001249-awn9ayy6 58 17 ( ( -LRB- cord-001249-awn9ayy6 58 18 MCS MCS NNP cord-001249-awn9ayy6 58 19 ) ) -RRB- cord-001249-awn9ayy6 58 20 of of IN cord-001249-awn9ayy6 58 21 pCAGGS pCAGGS NNP cord-001249-awn9ayy6 58 22 - - HYPH cord-001249-awn9ayy6 58 23 MCSII MCSII NNP cord-001249-awn9ayy6 58 24 ; ; : cord-001249-awn9ayy6 58 25 pCAGGs_MCSII_V5 pCAGGs_MCSII_V5 NNP cord-001249-awn9ayy6 58 26 was be VBD cord-001249-awn9ayy6 58 27 further further RB cord-001249-awn9ayy6 58 28 used use VBN cord-001249-awn9ayy6 58 29 to to TO cord-001249-awn9ayy6 58 30 generate generate VB cord-001249-awn9ayy6 58 31 pCAGGs_MCSII_PreGn_V5 pcaggs_mcsii_pregn_v5 NN cord-001249-awn9ayy6 58 32 , , , cord-001249-awn9ayy6 58 33 pCAGGs_MCSII_NS pcaggs_mcsii_ns NN cord-001249-awn9ayy6 58 34 M M NNP cord-001249-awn9ayy6 58 35 _ _ NNP cord-001249-awn9ayy6 58 36 V5 V5 NNP cord-001249-awn9ayy6 58 37 and and CC cord-001249-awn9ayy6 58 38 pCAGGs_MCSII_PreGc_V5 pcaggs_mcsii_pregc_v5 NN cord-001249-awn9ayy6 58 39 . . . cord-001249-awn9ayy6 59 1 These these DT cord-001249-awn9ayy6 59 2 constructs construct NNS cord-001249-awn9ayy6 59 3 were be VBD cord-001249-awn9ayy6 59 4 generated generate VBN cord-001249-awn9ayy6 59 5 by by IN cord-001249-awn9ayy6 59 6 amplification amplification NN cord-001249-awn9ayy6 59 7 of of IN cord-001249-awn9ayy6 59 8 appropriate appropriate JJ cord-001249-awn9ayy6 59 9 fragments fragment NNS cord-001249-awn9ayy6 59 10 of of IN cord-001249-awn9ayy6 59 11 the the DT cord-001249-awn9ayy6 59 12 GV GV NNP cord-001249-awn9ayy6 59 13 ( ( -LRB- cord-001249-awn9ayy6 59 14 i. i. NN cord-001249-awn9ayy6 59 15 : : : cord-001249-awn9ayy6 59 16 TA TA NNP cord-001249-awn9ayy6 59 17 - - HYPH cord-001249-awn9ayy6 59 18 TCATATCGATGGCAACCTCTTTAACTGCTTTTC TCATATCGATGGCAACCTCTTTAACTGCTTTTC NNP cord-001249-awn9ayy6 59 19 ) ) -RRB- cord-001249-awn9ayy6 59 20 and and CC cord-001249-awn9ayy6 59 21 pcDNA pcdna RB cord-001249-awn9ayy6 59 22 - - HYPH cord-001249-awn9ayy6 59 23 GV gv NN cord-001249-awn9ayy6 59 24 - - HYPH cord-001249-awn9ayy6 59 25 M M NNP cord-001249-awn9ayy6 59 26 [ [ -LRB- cord-001249-awn9ayy6 59 27 62 62 CD cord-001249-awn9ayy6 59 28 ] ] -RRB- cord-001249-awn9ayy6 59 29 as as IN cord-001249-awn9ayy6 59 30 template template NN cord-001249-awn9ayy6 59 31 . . . cord-001249-awn9ayy6 60 1 These these DT cord-001249-awn9ayy6 60 2 fragments fragment NNS cord-001249-awn9ayy6 60 3 were be VBD cord-001249-awn9ayy6 60 4 then then RB cord-001249-awn9ayy6 60 5 inserted insert VBN cord-001249-awn9ayy6 60 6 upstream upstream RB cord-001249-awn9ayy6 60 7 of of IN cord-001249-awn9ayy6 60 8 , , , cord-001249-awn9ayy6 60 9 and and CC cord-001249-awn9ayy6 60 10 in in IN cord-001249-awn9ayy6 60 11 frame frame NN cord-001249-awn9ayy6 60 12 with with IN cord-001249-awn9ayy6 60 13 , , , cord-001249-awn9ayy6 60 14 the the DT cord-001249-awn9ayy6 60 15 V5-tag v5-tag NN cord-001249-awn9ayy6 60 16 of of IN cord-001249-awn9ayy6 60 17 pCAGGs_MCSII_V5 pCAGGs_MCSII_V5 NNP cord-001249-awn9ayy6 60 18 . . . cord-001249-awn9ayy6 61 1 All all DT cord-001249-awn9ayy6 61 2 PCRs pcr NNS cord-001249-awn9ayy6 61 3 were be VBD cord-001249-awn9ayy6 61 4 performed perform VBN cord-001249-awn9ayy6 61 5 using use VBG cord-001249-awn9ayy6 61 6 proofreading proofreading NN cord-001249-awn9ayy6 61 7 polymerase polymerase NN cord-001249-awn9ayy6 61 8 ( ( -LRB- cord-001249-awn9ayy6 61 9 KOD KOD NNP cord-001249-awn9ayy6 61 10 ; ; : cord-001249-awn9ayy6 61 11 Novagen Novagen NNP cord-001249-awn9ayy6 61 12 ) ) -RRB- cord-001249-awn9ayy6 61 13 . . . cord-001249-awn9ayy6 62 1 All all DT cord-001249-awn9ayy6 62 2 inserts insert NNS cord-001249-awn9ayy6 62 3 were be VBD cord-001249-awn9ayy6 62 4 sequenced sequence VBN cord-001249-awn9ayy6 62 5 completely completely RB cord-001249-awn9ayy6 62 6 . . . cord-001249-awn9ayy6 63 1 The the DT cord-001249-awn9ayy6 63 2 generation generation NN cord-001249-awn9ayy6 63 3 of of IN cord-001249-awn9ayy6 63 4 specific specific JJ cord-001249-awn9ayy6 63 5 rabbit rabbit NN cord-001249-awn9ayy6 63 6 antisera antisera NN cord-001249-awn9ayy6 63 7 to to IN cord-001249-awn9ayy6 63 8 the the DT cord-001249-awn9ayy6 63 9 amino amino NN cord-001249-awn9ayy6 63 10 - - HYPH cord-001249-awn9ayy6 63 11 terminus terminus NN cord-001249-awn9ayy6 63 12 of of IN cord-001249-awn9ayy6 63 13 the the DT cord-001249-awn9ayy6 63 14 GV GV NNP cord-001249-awn9ayy6 63 15 ( ( -LRB- cord-001249-awn9ayy6 63 16 NSDVi NSDVi NNS cord-001249-awn9ayy6 63 17 ) ) -RRB- cord-001249-awn9ayy6 64 1 N N NNP cord-001249-awn9ayy6 64 2 protein protein NN cord-001249-awn9ayy6 64 3 will will MD cord-001249-awn9ayy6 64 4 be be VB cord-001249-awn9ayy6 64 5 described describe VBN cord-001249-awn9ayy6 64 6 in in IN cord-001249-awn9ayy6 64 7 detail detail NN cord-001249-awn9ayy6 64 8 elsewhere elsewhere RB cord-001249-awn9ayy6 64 9 . . . cord-001249-awn9ayy6 65 1 Briefly briefly RB cord-001249-awn9ayy6 65 2 , , , cord-001249-awn9ayy6 65 3 bacterially bacterially RB cord-001249-awn9ayy6 65 4 expressed express VBD cord-001249-awn9ayy6 65 5 proteins protein NNS cord-001249-awn9ayy6 65 6 consisting consist VBG cord-001249-awn9ayy6 65 7 of of IN cord-001249-awn9ayy6 65 8 the the DT cord-001249-awn9ayy6 65 9 first first JJ cord-001249-awn9ayy6 65 10 167 167 CD cord-001249-awn9ayy6 65 11 amino amino JJ cord-001249-awn9ayy6 65 12 acids acid NNS cord-001249-awn9ayy6 65 13 of of IN cord-001249-awn9ayy6 65 14 the the DT cord-001249-awn9ayy6 65 15 viral viral JJ cord-001249-awn9ayy6 65 16 N n CD cord-001249-awn9ayy6 65 17 protein protein NN cord-001249-awn9ayy6 65 18 or or CC cord-001249-awn9ayy6 65 19 the the DT cord-001249-awn9ayy6 65 20 first first JJ cord-001249-awn9ayy6 65 21 169 169 CD cord-001249-awn9ayy6 65 22 amino amino JJ cord-001249-awn9ayy6 65 23 acids acid NNS cord-001249-awn9ayy6 65 24 of of IN cord-001249-awn9ayy6 65 25 the the DT cord-001249-awn9ayy6 65 26 viral viral JJ cord-001249-awn9ayy6 65 27 L L NNP cord-001249-awn9ayy6 65 28 protein protein NN cord-001249-awn9ayy6 65 29 were be VBD cord-001249-awn9ayy6 65 30 used use VBN cord-001249-awn9ayy6 65 31 by by IN cord-001249-awn9ayy6 65 32 a a DT cord-001249-awn9ayy6 65 33 commercial commercial JJ cord-001249-awn9ayy6 65 34 company company NN cord-001249-awn9ayy6 65 35 to to TO cord-001249-awn9ayy6 65 36 immunise immunise VB cord-001249-awn9ayy6 65 37 four four CD cord-001249-awn9ayy6 65 38 rabbits rabbit NNS cord-001249-awn9ayy6 65 39 and and CC cord-001249-awn9ayy6 65 40 the the DT cord-001249-awn9ayy6 65 41 resulting result VBG cord-001249-awn9ayy6 65 42 sera sera NNP cord-001249-awn9ayy6 65 43 optimised optimise VBN cord-001249-awn9ayy6 65 44 for for IN cord-001249-awn9ayy6 65 45 immunofluorescence immunofluorescence NN cord-001249-awn9ayy6 65 46 microscopy microscopy NN cord-001249-awn9ayy6 65 47 . . . cord-001249-awn9ayy6 66 1 Mouse mouse NN cord-001249-awn9ayy6 66 2 monoclonal monoclonal JJ cord-001249-awn9ayy6 66 3 recognising recognise VBG cord-001249-awn9ayy6 66 4 the the DT cord-001249-awn9ayy6 66 5 denatured denature VBN cord-001249-awn9ayy6 66 6 form form NN cord-001249-awn9ayy6 66 7 of of IN cord-001249-awn9ayy6 66 8 the the DT cord-001249-awn9ayy6 66 9 PreGn PreGn NNP cord-001249-awn9ayy6 66 10 glycoprotein glycoprotein NN cord-001249-awn9ayy6 66 11 from from IN cord-001249-awn9ayy6 66 12 NSDVi NSDVi NNS cord-001249-awn9ayy6 66 13 , , , cord-001249-awn9ayy6 66 14 but but CC cord-001249-awn9ayy6 66 15 not not RB cord-001249-awn9ayy6 66 16 from from IN cord-001249-awn9ayy6 66 17 NSDVu NSDVu NNP cord-001249-awn9ayy6 66 18 , , , cord-001249-awn9ayy6 66 19 was be VBD cord-001249-awn9ayy6 66 20 obtained obtain VBN cord-001249-awn9ayy6 66 21 from from IN cord-001249-awn9ayy6 66 22 AbMart AbMart NNP cord-001249-awn9ayy6 66 23 . . . cord-001249-awn9ayy6 67 1 For for IN cord-001249-awn9ayy6 67 2 detection detection NN cord-001249-awn9ayy6 67 3 of of IN cord-001249-awn9ayy6 67 4 cellular cellular JJ cord-001249-awn9ayy6 67 5 markers marker NNS cord-001249-awn9ayy6 67 6 the the DT cord-001249-awn9ayy6 67 7 following follow VBG cord-001249-awn9ayy6 67 8 antibodies antibody NNS cord-001249-awn9ayy6 67 9 were be VBD cord-001249-awn9ayy6 67 10 used use VBN cord-001249-awn9ayy6 67 11 : : : cord-001249-awn9ayy6 67 12 mouse mouse NN cord-001249-awn9ayy6 67 13 monoclonal monoclonal JJ cord-001249-awn9ayy6 67 14 anti anti NNP cord-001249-awn9ayy6 67 15 - - NNP cord-001249-awn9ayy6 67 16 PDI PDI NNP cord-001249-awn9ayy6 68 1 [ [ -LRB- cord-001249-awn9ayy6 68 2 clone clone NN cord-001249-awn9ayy6 68 3 1D3 1d3 CD cord-001249-awn9ayy6 68 4 ] ] -RRB- cord-001249-awn9ayy6 68 5 was be VBD cord-001249-awn9ayy6 68 6 obtained obtain VBN cord-001249-awn9ayy6 68 7 from from IN cord-001249-awn9ayy6 68 8 Bioquote Bioquote NNP cord-001249-awn9ayy6 68 9 , , , cord-001249-awn9ayy6 68 10 mouse mouse NN cord-001249-awn9ayy6 68 11 monoclonal monoclonal JJ cord-001249-awn9ayy6 68 12 anti anti JJ cord-001249-awn9ayy6 68 13 - - JJ cord-001249-awn9ayy6 68 14 PDI pdi JJ cord-001249-awn9ayy6 68 15 [ [ -LRB- cord-001249-awn9ayy6 68 16 clone clone NN cord-001249-awn9ayy6 68 17 RL90 RL90 NNP cord-001249-awn9ayy6 68 18 ] ] -RRB- cord-001249-awn9ayy6 68 19 and and CC cord-001249-awn9ayy6 68 20 anti anti JJ cord-001249-awn9ayy6 68 21 - - JJ cord-001249-awn9ayy6 68 22 calnexin calnexin JJ cord-001249-awn9ayy6 68 23 [ [ -LRB- cord-001249-awn9ayy6 68 24 clone clone NN cord-001249-awn9ayy6 68 25 AF18 AF18 NNP cord-001249-awn9ayy6 68 26 ] ] -RRB- cord-001249-awn9ayy6 68 27 antibodies antibody NNS cord-001249-awn9ayy6 68 28 were be VBD cord-001249-awn9ayy6 68 29 purchased purchase VBN cord-001249-awn9ayy6 68 30 from from IN cord-001249-awn9ayy6 68 31 Abcam Abcam NNP cord-001249-awn9ayy6 68 32 ; ; : cord-001249-awn9ayy6 68 33 mouse mouse NN cord-001249-awn9ayy6 68 34 monoclonal monoclonal JJ cord-001249-awn9ayy6 68 35 Anti Anti NNP cord-001249-awn9ayy6 68 36 V5-Tag V5-Tag NNP cord-001249-awn9ayy6 68 37 : : : cord-001249-awn9ayy6 69 1 Alexa Alexa NNP cord-001249-awn9ayy6 69 2 Fluor Fluor NNP cord-001249-awn9ayy6 69 3 488 488 CD cord-001249-awn9ayy6 69 4 was be VBD cord-001249-awn9ayy6 69 5 obtained obtain VBN cord-001249-awn9ayy6 69 6 from from IN cord-001249-awn9ayy6 69 7 AbDSerotec abdserotec NN cord-001249-awn9ayy6 69 8 , , , cord-001249-awn9ayy6 69 9 mouse mouse NN cord-001249-awn9ayy6 69 10 monoclonal monoclonal JJ cord-001249-awn9ayy6 69 11 anti anti JJ cord-001249-awn9ayy6 69 12 - - JJ cord-001249-awn9ayy6 69 13 a a DT cord-001249-awn9ayy6 69 14 - - HYPH cord-001249-awn9ayy6 69 15 tubulin tubulin NN cord-001249-awn9ayy6 69 16 from from IN cord-001249-awn9ayy6 69 17 Sigma Sigma NNP cord-001249-awn9ayy6 69 18 , , , cord-001249-awn9ayy6 69 19 mouse mouse NN cord-001249-awn9ayy6 69 20 monoclonal monoclonal JJ cord-001249-awn9ayy6 69 21 anti anti NNP cord-001249-awn9ayy6 69 22 - - NNP cord-001249-awn9ayy6 69 23 GM130 GM130 NNP cord-001249-awn9ayy6 69 24 from from IN cord-001249-awn9ayy6 69 25 BD BD NNP cord-001249-awn9ayy6 69 26 Bioscience Bioscience NNP cord-001249-awn9ayy6 69 27 , , , cord-001249-awn9ayy6 69 28 mouse mouse NN cord-001249-awn9ayy6 69 29 monoclonal monoclonal JJ cord-001249-awn9ayy6 69 30 anti anti JJ cord-001249-awn9ayy6 69 31 - - JJ cord-001249-awn9ayy6 69 32 PCNA PCNA NNP cord-001249-awn9ayy6 69 33 ( ( -LRB- cord-001249-awn9ayy6 69 34 PC10 PC10 NNP cord-001249-awn9ayy6 69 35 ) ) -RRB- cord-001249-awn9ayy6 69 36 from from IN cord-001249-awn9ayy6 69 37 Santa Santa NNP cord-001249-awn9ayy6 69 38 Cruz Cruz NNP cord-001249-awn9ayy6 69 39 Biotechnology Biotechnology NNP cord-001249-awn9ayy6 69 40 . . . cord-001249-awn9ayy6 70 1 Alexa Alexa NNP cord-001249-awn9ayy6 70 2 Fluor Fluor NNP cord-001249-awn9ayy6 70 3 488 488 CD cord-001249-awn9ayy6 70 4 goat goat NN cord-001249-awn9ayy6 70 5 anti anti JJ cord-001249-awn9ayy6 70 6 - - JJ cord-001249-awn9ayy6 70 7 mouse mouse JJ cord-001249-awn9ayy6 70 8 IgG igg NN cord-001249-awn9ayy6 70 9 ( ( -LRB- cord-001249-awn9ayy6 70 10 H+L H+L NNP cord-001249-awn9ayy6 70 11 ) ) -RRB- cord-001249-awn9ayy6 70 12 , , , cord-001249-awn9ayy6 70 13 Alexa Alexa NNP cord-001249-awn9ayy6 70 14 Fluor Fluor NNP cord-001249-awn9ayy6 70 15 568 568 CD cord-001249-awn9ayy6 70 16 goat goat NN cord-001249-awn9ayy6 70 17 anti anti JJ cord-001249-awn9ayy6 70 18 - - JJ cord-001249-awn9ayy6 70 19 mouse mouse JJ cord-001249-awn9ayy6 70 20 IgG igg NN cord-001249-awn9ayy6 70 21 ( ( -LRB- cord-001249-awn9ayy6 70 22 H+L H+L NNP cord-001249-awn9ayy6 70 23 ) ) -RRB- cord-001249-awn9ayy6 70 24 , , , cord-001249-awn9ayy6 70 25 Alexa Alexa NNP cord-001249-awn9ayy6 70 26 Fluor Fluor NNP cord-001249-awn9ayy6 71 1 488 488 CD cord-001249-awn9ayy6 71 2 goat goat NN cord-001249-awn9ayy6 71 3 anti anti JJ cord-001249-awn9ayy6 71 4 - - JJ cord-001249-awn9ayy6 71 5 rat rat JJ cord-001249-awn9ayy6 71 6 IgG igg NN cord-001249-awn9ayy6 71 7 ( ( -LRB- cord-001249-awn9ayy6 71 8 H+L H+L NNP cord-001249-awn9ayy6 71 9 ) ) -RRB- cord-001249-awn9ayy6 71 10 , , , cord-001249-awn9ayy6 71 11 Alexa Alexa NNP cord-001249-awn9ayy6 71 12 Fluor Fluor NNP cord-001249-awn9ayy6 71 13 568 568 CD cord-001249-awn9ayy6 71 14 goat goat NN cord-001249-awn9ayy6 71 15 anti anti JJ cord-001249-awn9ayy6 71 16 - - JJ cord-001249-awn9ayy6 71 17 rabbit rabbit JJ cord-001249-awn9ayy6 71 18 IgG igg NN cord-001249-awn9ayy6 71 19 ( ( -LRB- cord-001249-awn9ayy6 71 20 H+L H+L NNP cord-001249-awn9ayy6 71 21 ) ) -RRB- cord-001249-awn9ayy6 71 22 and and CC cord-001249-awn9ayy6 71 23 Zenon Zenon NNP cord-001249-awn9ayy6 71 24 Rabbit Rabbit NNP cord-001249-awn9ayy6 71 25 IgG igg NN cord-001249-awn9ayy6 71 26 Labelling Labelling NNP cord-001249-awn9ayy6 71 27 Kit Kit NNP cord-001249-awn9ayy6 71 28 were be VBD cord-001249-awn9ayy6 71 29 purchased purchase VBN cord-001249-awn9ayy6 71 30 from from IN cord-001249-awn9ayy6 71 31 Life Life NNP cord-001249-awn9ayy6 71 32 Technologies Technologies NNPS cord-001249-awn9ayy6 71 33 . . . cord-001249-awn9ayy6 72 1 Rat Rat NNP cord-001249-awn9ayy6 72 2 anti anti JJ cord-001249-awn9ayy6 72 3 - - JJ cord-001249-awn9ayy6 72 4 p102 p102 JJ cord-001249-awn9ayy6 72 5 antibody antibody NN cord-001249-awn9ayy6 72 6 ( ( -LRB- cord-001249-awn9ayy6 72 7 clone clone NN cord-001249-awn9ayy6 72 8 23C 23c CD cord-001249-awn9ayy6 72 9 ) ) -RRB- cord-001249-awn9ayy6 72 10 , , , cord-001249-awn9ayy6 72 11 was be VBD cord-001249-awn9ayy6 72 12 prepared prepare VBN cord-001249-awn9ayy6 72 13 by by IN cord-001249-awn9ayy6 72 14 the the DT cord-001249-awn9ayy6 72 15 Institute Institute NNP cord-001249-awn9ayy6 72 16 of of IN cord-001249-awn9ayy6 72 17 Cancer Cancer NNP cord-001249-awn9ayy6 72 18 Research Research NNP cord-001249-awn9ayy6 72 19 [ [ -LRB- cord-001249-awn9ayy6 72 20 63 63 CD cord-001249-awn9ayy6 72 21 ] ] -RRB- cord-001249-awn9ayy6 72 22 and and CC cord-001249-awn9ayy6 72 23 was be VBD cord-001249-awn9ayy6 72 24 kindly kindly RB cord-001249-awn9ayy6 72 25 given give VBN cord-001249-awn9ayy6 72 26 to to IN cord-001249-awn9ayy6 72 27 us -PRON- PRP cord-001249-awn9ayy6 72 28 by by IN cord-001249-awn9ayy6 72 29 Dr Dr NNP cord-001249-awn9ayy6 72 30 Philippa Philippa NNP cord-001249-awn9ayy6 72 31 Hawes Hawes NNP cord-001249-awn9ayy6 72 32 , , , cord-001249-awn9ayy6 72 33 Pirbright Pirbright NNP cord-001249-awn9ayy6 72 34 Institute Institute NNP cord-001249-awn9ayy6 72 35 ; ; : cord-001249-awn9ayy6 72 36 antibody antibody NN cord-001249-awn9ayy6 72 37 to to IN cord-001249-awn9ayy6 72 38 ERp57 ERp57 NNS cord-001249-awn9ayy6 72 39 has have VBZ cord-001249-awn9ayy6 72 40 been be VBN cord-001249-awn9ayy6 72 41 described describe VBN cord-001249-awn9ayy6 72 42 previously previously RB cord-001249-awn9ayy6 72 43 [ [ -LRB- cord-001249-awn9ayy6 72 44 64 64 CD cord-001249-awn9ayy6 72 45 ] ] -RRB- cord-001249-awn9ayy6 72 46 and and CC cord-001249-awn9ayy6 72 47 was be VBD cord-001249-awn9ayy6 72 48 kindly kindly RB cord-001249-awn9ayy6 72 49 given give VBN cord-001249-awn9ayy6 72 50 to to IN cord-001249-awn9ayy6 72 51 us -PRON- PRP cord-001249-awn9ayy6 72 52 by by IN cord-001249-awn9ayy6 72 53 Dr Dr NNP cord-001249-awn9ayy6 72 54 Christopher Christopher NNP cord-001249-awn9ayy6 72 55 Netherton Netherton NNP cord-001249-awn9ayy6 72 56 , , , cord-001249-awn9ayy6 72 57 Pirbright Pirbright NNP cord-001249-awn9ayy6 72 58 Institute Institute NNP cord-001249-awn9ayy6 72 59 . . . cord-001249-awn9ayy6 73 1 Vero Vero NNP cord-001249-awn9ayy6 73 2 cells cell NNS cord-001249-awn9ayy6 73 3 or or CC cord-001249-awn9ayy6 73 4 CJE cje NN cord-001249-awn9ayy6 73 5 cells cell NNS cord-001249-awn9ayy6 73 6 were be VBD cord-001249-awn9ayy6 73 7 plated plate VBN cord-001249-awn9ayy6 73 8 at at IN cord-001249-awn9ayy6 73 9 an an DT cord-001249-awn9ayy6 73 10 initial initial JJ cord-001249-awn9ayy6 73 11 seeding seed VBG cord-001249-awn9ayy6 73 12 density density NN cord-001249-awn9ayy6 73 13 of of IN cord-001249-awn9ayy6 73 14 4 4 CD cord-001249-awn9ayy6 73 15 x10 x10 CD cord-001249-awn9ayy6 73 16 4 4 CD cord-001249-awn9ayy6 73 17 cells cell NNS cord-001249-awn9ayy6 73 18 / / SYM cord-001249-awn9ayy6 73 19 well well RB cord-001249-awn9ayy6 73 20 in in IN cord-001249-awn9ayy6 73 21 12-well 12-well CD cord-001249-awn9ayy6 73 22 plates plate NNS cord-001249-awn9ayy6 73 23 . . . cord-001249-awn9ayy6 74 1 On on IN cord-001249-awn9ayy6 74 2 the the DT cord-001249-awn9ayy6 74 3 next next JJ cord-001249-awn9ayy6 74 4 day day NN cord-001249-awn9ayy6 74 5 , , , cord-001249-awn9ayy6 74 6 cells cell NNS cord-001249-awn9ayy6 74 7 were be VBD cord-001249-awn9ayy6 74 8 infected infect VBN cord-001249-awn9ayy6 74 9 with with IN cord-001249-awn9ayy6 74 10 NSDVi NSDVi NNS cord-001249-awn9ayy6 74 11 or or CC cord-001249-awn9ayy6 74 12 NSDVu NSDVu NNPS cord-001249-awn9ayy6 74 13 at at IN cord-001249-awn9ayy6 74 14 a a DT cord-001249-awn9ayy6 74 15 multiplicity multiplicity NN cord-001249-awn9ayy6 74 16 of of IN cord-001249-awn9ayy6 74 17 infection infection NN cord-001249-awn9ayy6 74 18 indicated indicate VBN cord-001249-awn9ayy6 74 19 for for IN cord-001249-awn9ayy6 74 20 each each DT cord-001249-awn9ayy6 74 21 experiment experiment NN cord-001249-awn9ayy6 74 22 . . . cord-001249-awn9ayy6 75 1 The the DT cord-001249-awn9ayy6 75 2 virus virus NN cord-001249-awn9ayy6 75 3 inoculum inoculum NN cord-001249-awn9ayy6 75 4 was be VBD cord-001249-awn9ayy6 75 5 removed remove VBN cord-001249-awn9ayy6 75 6 after after IN cord-001249-awn9ayy6 75 7 one one CD cord-001249-awn9ayy6 75 8 hour hour NN cord-001249-awn9ayy6 75 9 , , , cord-001249-awn9ayy6 75 10 cells cell NNS cord-001249-awn9ayy6 75 11 were be VBD cord-001249-awn9ayy6 75 12 washed wash VBN cord-001249-awn9ayy6 75 13 once once RB cord-001249-awn9ayy6 75 14 with with IN cord-001249-awn9ayy6 75 15 phosphate phosphate NN cord-001249-awn9ayy6 75 16 buffered buffer VBN cord-001249-awn9ayy6 75 17 saline saline NN cord-001249-awn9ayy6 75 18 ( ( -LRB- cord-001249-awn9ayy6 75 19 PBS PBS NNP cord-001249-awn9ayy6 75 20 ) ) -RRB- cord-001249-awn9ayy6 75 21 and and CC cord-001249-awn9ayy6 75 22 fresh fresh JJ cord-001249-awn9ayy6 75 23 medium medium NN cord-001249-awn9ayy6 75 24 was be VBD cord-001249-awn9ayy6 75 25 added add VBN cord-001249-awn9ayy6 75 26 . . . cord-001249-awn9ayy6 76 1 The the DT cord-001249-awn9ayy6 76 2 infected infected JJ cord-001249-awn9ayy6 76 3 cells cell NNS cord-001249-awn9ayy6 76 4 were be VBD cord-001249-awn9ayy6 76 5 further further RB cord-001249-awn9ayy6 76 6 incubated incubate VBN cord-001249-awn9ayy6 76 7 for for IN cord-001249-awn9ayy6 76 8 the the DT cord-001249-awn9ayy6 76 9 times time NNS cord-001249-awn9ayy6 76 10 indicated indicate VBN cord-001249-awn9ayy6 76 11 for for IN cord-001249-awn9ayy6 76 12 each each DT cord-001249-awn9ayy6 76 13 individual individual JJ cord-001249-awn9ayy6 76 14 experiment experiment NN cord-001249-awn9ayy6 76 15 . . . cord-001249-awn9ayy6 77 1 All all DT cord-001249-awn9ayy6 77 2 transfections transfection NNS cord-001249-awn9ayy6 77 3 were be VBD cord-001249-awn9ayy6 77 4 carried carry VBN cord-001249-awn9ayy6 77 5 out out RP cord-001249-awn9ayy6 77 6 with with IN cord-001249-awn9ayy6 77 7 TransIT TransIT NNP cord-001249-awn9ayy6 77 8 - - HYPH cord-001249-awn9ayy6 77 9 LT1 LT1 NNP cord-001249-awn9ayy6 77 10 ( ( -LRB- cord-001249-awn9ayy6 77 11 Mirus Mirus NNP cord-001249-awn9ayy6 77 12 ) ) -RRB- cord-001249-awn9ayy6 77 13 according accord VBG cord-001249-awn9ayy6 77 14 to to IN cord-001249-awn9ayy6 77 15 the the DT cord-001249-awn9ayy6 77 16 manufacturer manufacturer NN cord-001249-awn9ayy6 77 17 's 's POS cord-001249-awn9ayy6 77 18 instructions instruction NNS cord-001249-awn9ayy6 77 19 . . . cord-001249-awn9ayy6 78 1 A a DT cord-001249-awn9ayy6 78 2 ratio ratio NN cord-001249-awn9ayy6 78 3 of of IN cord-001249-awn9ayy6 78 4 2 2 CD cord-001249-awn9ayy6 78 5 ml ml NNP cord-001249-awn9ayy6 78 6 TransIT transit CD cord-001249-awn9ayy6 78 7 - - HYPH cord-001249-awn9ayy6 78 8 LT1 lt1 NN cord-001249-awn9ayy6 78 9 reagent reagent NN cord-001249-awn9ayy6 78 10 per per IN cord-001249-awn9ayy6 78 11 1 1 CD cord-001249-awn9ayy6 78 12 mg mg NN cord-001249-awn9ayy6 78 13 of of IN cord-001249-awn9ayy6 78 14 DNA DNA NNP cord-001249-awn9ayy6 78 15 was be VBD cord-001249-awn9ayy6 78 16 used use VBN cord-001249-awn9ayy6 78 17 ; ; : cord-001249-awn9ayy6 78 18 Vero Vero NNP cord-001249-awn9ayy6 78 19 cells cell NNS cord-001249-awn9ayy6 78 20 were be VBD cord-001249-awn9ayy6 78 21 plated plate VBN cord-001249-awn9ayy6 78 22 as as IN cord-001249-awn9ayy6 78 23 described describe VBN cord-001249-awn9ayy6 78 24 above above RB cord-001249-awn9ayy6 78 25 , , , cord-001249-awn9ayy6 78 26 usually usually RB cord-001249-awn9ayy6 78 27 1 1 CD cord-001249-awn9ayy6 78 28 day day NN cord-001249-awn9ayy6 78 29 prior prior RB cord-001249-awn9ayy6 78 30 to to IN cord-001249-awn9ayy6 78 31 transfection transfection NN cord-001249-awn9ayy6 78 32 . . . cord-001249-awn9ayy6 79 1 Vero Vero NNP cord-001249-awn9ayy6 79 2 cells cell NNS cord-001249-awn9ayy6 79 3 were be VBD cord-001249-awn9ayy6 79 4 plated plate VBN cord-001249-awn9ayy6 79 5 as as IN cord-001249-awn9ayy6 79 6 described describe VBN cord-001249-awn9ayy6 79 7 above above RB cord-001249-awn9ayy6 79 8 on on IN cord-001249-awn9ayy6 79 9 18 18 CD cord-001249-awn9ayy6 79 10 mm mm NN cord-001249-awn9ayy6 79 11 - - HYPH cord-001249-awn9ayy6 79 12 diameter diameter NN cord-001249-awn9ayy6 79 13 coverslips coverslip NNS cord-001249-awn9ayy6 79 14 prior prior RB cord-001249-awn9ayy6 79 15 to to IN cord-001249-awn9ayy6 79 16 infection infection NN cord-001249-awn9ayy6 79 17 or or CC cord-001249-awn9ayy6 79 18 transfection transfection NN cord-001249-awn9ayy6 79 19 . . . cord-001249-awn9ayy6 80 1 At at IN cord-001249-awn9ayy6 80 2 the the DT cord-001249-awn9ayy6 80 3 indicated indicate VBN cord-001249-awn9ayy6 80 4 times time NNS cord-001249-awn9ayy6 80 5 , , , cord-001249-awn9ayy6 80 6 cells cell NNS cord-001249-awn9ayy6 80 7 were be VBD cord-001249-awn9ayy6 80 8 fixed fix VBN cord-001249-awn9ayy6 80 9 with with IN cord-001249-awn9ayy6 80 10 3 3 CD cord-001249-awn9ayy6 80 11 % % NN cord-001249-awn9ayy6 80 12 paraformaldehyde paraformaldehyde JJ cord-001249-awn9ayy6 80 13 ( ( -LRB- cord-001249-awn9ayy6 80 14 PFA PFA NNP cord-001249-awn9ayy6 80 15 ) ) -RRB- cord-001249-awn9ayy6 80 16 and and CC cord-001249-awn9ayy6 80 17 then then RB cord-001249-awn9ayy6 80 18 briefly briefly RB cord-001249-awn9ayy6 80 19 permeabilised permeabilise VBD cord-001249-awn9ayy6 80 20 with with IN cord-001249-awn9ayy6 80 21 ice ice NN cord-001249-awn9ayy6 80 22 - - HYPH cord-001249-awn9ayy6 80 23 cold cold JJ cord-001249-awn9ayy6 80 24 100 100 CD cord-001249-awn9ayy6 80 25 % % NN cord-001249-awn9ayy6 80 26 methanol methanol NN cord-001249-awn9ayy6 80 27 . . . cord-001249-awn9ayy6 81 1 The the DT cord-001249-awn9ayy6 81 2 fixed fixed JJ cord-001249-awn9ayy6 81 3 and and CC cord-001249-awn9ayy6 81 4 permeabilised permeabilised JJ cord-001249-awn9ayy6 81 5 cells cell NNS cord-001249-awn9ayy6 81 6 were be VBD cord-001249-awn9ayy6 81 7 blocked block VBN cord-001249-awn9ayy6 81 8 with with IN cord-001249-awn9ayy6 81 9 0.2 0.2 CD cord-001249-awn9ayy6 81 10 % % NN cord-001249-awn9ayy6 81 11 porcine porcine JJ cord-001249-awn9ayy6 81 12 gelatine gelatine NN cord-001249-awn9ayy6 81 13 for for IN cord-001249-awn9ayy6 81 14 at at RB cord-001249-awn9ayy6 81 15 least least RBS cord-001249-awn9ayy6 81 16 30 30 CD cord-001249-awn9ayy6 81 17 min min NN cord-001249-awn9ayy6 81 18 before before IN cord-001249-awn9ayy6 81 19 processing process VBG cord-001249-awn9ayy6 81 20 for for IN cord-001249-awn9ayy6 81 21 antibody antibody NN cord-001249-awn9ayy6 81 22 staining staining NN cord-001249-awn9ayy6 81 23 . . . cord-001249-awn9ayy6 82 1 For for IN cord-001249-awn9ayy6 82 2 detection detection NN cord-001249-awn9ayy6 82 3 of of IN cord-001249-awn9ayy6 82 4 surface surface NN cord-001249-awn9ayy6 82 5 proteins protein NNS cord-001249-awn9ayy6 82 6 , , , cord-001249-awn9ayy6 82 7 Vero Vero NNP cord-001249-awn9ayy6 82 8 cells cell NNS cord-001249-awn9ayy6 82 9 were be VBD cord-001249-awn9ayy6 82 10 fixed fix VBN cord-001249-awn9ayy6 82 11 with with IN cord-001249-awn9ayy6 82 12 3 3 CD cord-001249-awn9ayy6 82 13 % % NN cord-001249-awn9ayy6 82 14 PFA PFA NNP cord-001249-awn9ayy6 82 15 , , , cord-001249-awn9ayy6 82 16 blocked block VBD cord-001249-awn9ayy6 82 17 for for IN cord-001249-awn9ayy6 82 18 30 30 CD cord-001249-awn9ayy6 82 19 min min NN cord-001249-awn9ayy6 82 20 with with IN cord-001249-awn9ayy6 82 21 0.2 0.2 CD cord-001249-awn9ayy6 82 22 % % NN cord-001249-awn9ayy6 82 23 porcine porcine JJ cord-001249-awn9ayy6 82 24 gelatine gelatine NN cord-001249-awn9ayy6 82 25 and and CC cord-001249-awn9ayy6 82 26 incubated incubate VBN cord-001249-awn9ayy6 82 27 with with IN cord-001249-awn9ayy6 82 28 primary primary JJ cord-001249-awn9ayy6 82 29 antibody antibody NN cord-001249-awn9ayy6 82 30 against against IN cord-001249-awn9ayy6 82 31 PDI PDI NNP cord-001249-awn9ayy6 82 32 or or CC cord-001249-awn9ayy6 82 33 ERp57 ERp57 NNS cord-001249-awn9ayy6 82 34 . . . cord-001249-awn9ayy6 83 1 After after IN cord-001249-awn9ayy6 83 2 washing wash VBG cord-001249-awn9ayy6 83 3 with with IN cord-001249-awn9ayy6 83 4 PBS PBS NNP cord-001249-awn9ayy6 83 5 to to TO cord-001249-awn9ayy6 83 6 remove remove VB cord-001249-awn9ayy6 83 7 unbound unbound JJ cord-001249-awn9ayy6 83 8 antibodies antibody NNS cord-001249-awn9ayy6 83 9 , , , cord-001249-awn9ayy6 83 10 the the DT cord-001249-awn9ayy6 83 11 cells cell NNS cord-001249-awn9ayy6 83 12 were be VBD cord-001249-awn9ayy6 83 13 again again RB cord-001249-awn9ayy6 83 14 fixed fix VBN cord-001249-awn9ayy6 83 15 with with IN cord-001249-awn9ayy6 83 16 3 3 CD cord-001249-awn9ayy6 83 17 % % NN cord-001249-awn9ayy6 83 18 PFA PFA NNP cord-001249-awn9ayy6 83 19 , , , cord-001249-awn9ayy6 83 20 treated treat VBN cord-001249-awn9ayy6 83 21 briefly briefly RB cord-001249-awn9ayy6 83 22 with with IN cord-001249-awn9ayy6 83 23 ice ice NN cord-001249-awn9ayy6 83 24 - - HYPH cord-001249-awn9ayy6 83 25 cold cold JJ cord-001249-awn9ayy6 83 26 100 100 CD cord-001249-awn9ayy6 83 27 % % NN cord-001249-awn9ayy6 83 28 methanol methanol NN cord-001249-awn9ayy6 83 29 and and CC cord-001249-awn9ayy6 83 30 again again RB cord-001249-awn9ayy6 83 31 blocked block VBN cord-001249-awn9ayy6 83 32 with with IN cord-001249-awn9ayy6 83 33 the the DT cord-001249-awn9ayy6 83 34 0.2 0.2 CD cord-001249-awn9ayy6 83 35 % % NN cord-001249-awn9ayy6 83 36 porcine porcine JJ cord-001249-awn9ayy6 83 37 gelatine gelatine NN cord-001249-awn9ayy6 83 38 . . . cord-001249-awn9ayy6 84 1 Such such JJ cord-001249-awn9ayy6 84 2 permeabilised permeabilised JJ cord-001249-awn9ayy6 84 3 cells cell NNS cord-001249-awn9ayy6 84 4 were be VBD cord-001249-awn9ayy6 84 5 incubated incubate VBN cord-001249-awn9ayy6 84 6 with with IN cord-001249-awn9ayy6 84 7 anti anti JJ cord-001249-awn9ayy6 84 8 - - JJ cord-001249-awn9ayy6 84 9 NSDV nsdv JJ cord-001249-awn9ayy6 84 10 antibodies antibody NNS cord-001249-awn9ayy6 84 11 , , , cord-001249-awn9ayy6 84 12 and and CC cord-001249-awn9ayy6 84 13 then then RB cord-001249-awn9ayy6 84 14 proteins protein NNS cord-001249-awn9ayy6 84 15 were be VBD cord-001249-awn9ayy6 84 16 visualised visualise VBN cord-001249-awn9ayy6 84 17 with with IN cord-001249-awn9ayy6 84 18 fluorophore fluorophore NN cord-001249-awn9ayy6 84 19 - - HYPH cord-001249-awn9ayy6 84 20 conjugated conjugate VBN cord-001249-awn9ayy6 84 21 secondary secondary JJ cord-001249-awn9ayy6 84 22 antibodies antibody NNS cord-001249-awn9ayy6 84 23 . . . cord-001249-awn9ayy6 85 1 Initial initial JJ cord-001249-awn9ayy6 85 2 confocal confocal JJ cord-001249-awn9ayy6 85 3 images image NNS cord-001249-awn9ayy6 85 4 were be VBD cord-001249-awn9ayy6 85 5 obtained obtain VBN cord-001249-awn9ayy6 85 6 using use VBG cord-001249-awn9ayy6 85 7 sequential sequential JJ cord-001249-awn9ayy6 85 8 scanning scanning NN cord-001249-awn9ayy6 85 9 and and CC cord-001249-awn9ayy6 85 10 DMIRE2 DMIRE2 VBZ cord-001249-awn9ayy6 85 11 Leica Leica NNP cord-001249-awn9ayy6 85 12 CLSM CLSM NNP cord-001249-awn9ayy6 85 13 TCS TCS NNP cord-001249-awn9ayy6 85 14 - - HYPH cord-001249-awn9ayy6 85 15 SP2 SP2 NNP cord-001249-awn9ayy6 85 16 confocal confocal JJ cord-001249-awn9ayy6 85 17 laser laser NN cord-001249-awn9ayy6 85 18 scanning scanning NN cord-001249-awn9ayy6 85 19 microscope microscope NN cord-001249-awn9ayy6 85 20 . . . cord-001249-awn9ayy6 86 1 The the DT cord-001249-awn9ayy6 86 2 obtained obtain VBN cord-001249-awn9ayy6 86 3 TIFF tiff NN cord-001249-awn9ayy6 86 4 images image NNS cord-001249-awn9ayy6 86 5 were be VBD cord-001249-awn9ayy6 86 6 resized resize VBN cord-001249-awn9ayy6 86 7 and and CC cord-001249-awn9ayy6 86 8 overlays overlay NNS cord-001249-awn9ayy6 86 9 were be VBD cord-001249-awn9ayy6 86 10 prepared prepare VBN cord-001249-awn9ayy6 86 11 with with IN cord-001249-awn9ayy6 86 12 Adobe Adobe NNP cord-001249-awn9ayy6 86 13 Photoshop Photoshop NNP cord-001249-awn9ayy6 86 14 . . . cord-001249-awn9ayy6 87 1 Statistical statistical JJ cord-001249-awn9ayy6 87 2 analysis analysis NN cord-001249-awn9ayy6 87 3 of of IN cord-001249-awn9ayy6 87 4 the the DT cord-001249-awn9ayy6 87 5 effect effect NN cord-001249-awn9ayy6 87 6 of of IN cord-001249-awn9ayy6 87 7 infection infection NN cord-001249-awn9ayy6 87 8 on on IN cord-001249-awn9ayy6 87 9 distribution distribution NN cord-001249-awn9ayy6 87 10 of of IN cord-001249-awn9ayy6 87 11 host host NN cord-001249-awn9ayy6 87 12 cell cell NN cord-001249-awn9ayy6 87 13 proteins protein NNS cord-001249-awn9ayy6 87 14 was be VBD cord-001249-awn9ayy6 87 15 carried carry VBN cord-001249-awn9ayy6 87 16 out out RP cord-001249-awn9ayy6 87 17 using use VBG cord-001249-awn9ayy6 87 18 the the DT cord-001249-awn9ayy6 87 19 chisq.test chisq.t JJS cord-001249-awn9ayy6 87 20 ( ( -LRB- cord-001249-awn9ayy6 87 21 ) ) -RRB- cord-001249-awn9ayy6 87 22 function function VB cord-001249-awn9ayy6 87 23 in in IN cord-001249-awn9ayy6 87 24 R. R. NNP cord-001249-awn9ayy6 87 25 NSDVi NSDVi NNS cord-001249-awn9ayy6 87 26 - - HYPH cord-001249-awn9ayy6 87 27 infected infect VBN cord-001249-awn9ayy6 87 28 cells cell NNS cord-001249-awn9ayy6 87 29 were be VBD cord-001249-awn9ayy6 87 30 harvested harvest VBN cord-001249-awn9ayy6 87 31 with with IN cord-001249-awn9ayy6 87 32 0.4 0.4 CD cord-001249-awn9ayy6 87 33 ml ml NNS cord-001249-awn9ayy6 87 34 of of IN cord-001249-awn9ayy6 87 35 a a DT cord-001249-awn9ayy6 87 36 lysis lysis NN cord-001249-awn9ayy6 87 37 buffer buffer NN cord-001249-awn9ayy6 87 38 ( ( -LRB- cord-001249-awn9ayy6 87 39 1 1 CD cord-001249-awn9ayy6 87 40 % % NN cord-001249-awn9ayy6 87 41 ( ( -LRB- cord-001249-awn9ayy6 87 42 v v NN cord-001249-awn9ayy6 87 43 / / SYM cord-001249-awn9ayy6 87 44 v v NN cord-001249-awn9ayy6 87 45 ) ) -RRB- cord-001249-awn9ayy6 87 46 Nonidet Nonidet NNP cord-001249-awn9ayy6 87 47 P-40 P-40 NNP cord-001249-awn9ayy6 87 48 , , , cord-001249-awn9ayy6 87 49 50 50 CD cord-001249-awn9ayy6 87 50 mM mm CD cord-001249-awn9ayy6 87 51 Tris Tris NNP cord-001249-awn9ayy6 87 52 / / SYM cord-001249-awn9ayy6 87 53 Cl Cl NNP cord-001249-awn9ayy6 87 54 pH pH NNP cord-001249-awn9ayy6 87 55 7.5 7.5 CD cord-001249-awn9ayy6 87 56 , , , cord-001249-awn9ayy6 87 57 150 150 CD cord-001249-awn9ayy6 87 58 mM mm CD cord-001249-awn9ayy6 87 59 NaCl nacl NN cord-001249-awn9ayy6 87 60 , , , cord-001249-awn9ayy6 87 61 2 2 CD cord-001249-awn9ayy6 87 62 mM mm CD cord-001249-awn9ayy6 87 63 EDTA EDTA NNP cord-001249-awn9ayy6 87 64 , , , cord-001249-awn9ayy6 87 65 2.5 2.5 CD cord-001249-awn9ayy6 87 66 mM mm CD cord-001249-awn9ayy6 87 67 EDTA EDTA NNP cord-001249-awn9ayy6 87 68 ) ) -RRB- cord-001249-awn9ayy6 87 69 containing contain VBG cord-001249-awn9ayy6 87 70 protease protease NN cord-001249-awn9ayy6 87 71 inhibitor inhibitor NN cord-001249-awn9ayy6 87 72 cocktail cocktail NN cord-001249-awn9ayy6 87 73 set set VBN cord-001249-awn9ayy6 87 74 III III NNP cord-001249-awn9ayy6 87 75 ( ( -LRB- cord-001249-awn9ayy6 87 76 Calbiochem Calbiochem NNP cord-001249-awn9ayy6 87 77 ) ) -RRB- cord-001249-awn9ayy6 87 78 at at IN cord-001249-awn9ayy6 87 79 a a DT cord-001249-awn9ayy6 87 80 final final JJ cord-001249-awn9ayy6 87 81 dilution dilution NN cord-001249-awn9ayy6 87 82 of of IN cord-001249-awn9ayy6 87 83 1/200 1/200 CD cord-001249-awn9ayy6 87 84 . . . cord-001249-awn9ayy6 88 1 Lysates Lysates NNP cord-001249-awn9ayy6 88 2 were be VBD cord-001249-awn9ayy6 88 3 centrifuged centrifuge VBN cord-001249-awn9ayy6 88 4 at at IN cord-001249-awn9ayy6 88 5 10,000 10,000 CD cord-001249-awn9ayy6 88 6 g g NN cord-001249-awn9ayy6 88 7 for for IN cord-001249-awn9ayy6 88 8 30 30 CD cord-001249-awn9ayy6 88 9 min min NN cord-001249-awn9ayy6 88 10 at at IN cord-001249-awn9ayy6 88 11 4C 4c CD cord-001249-awn9ayy6 88 12 and and CC cord-001249-awn9ayy6 88 13 the the DT cord-001249-awn9ayy6 88 14 supernatants supernatant NNS cord-001249-awn9ayy6 88 15 were be VBD cord-001249-awn9ayy6 88 16 subjected subject VBN cord-001249-awn9ayy6 88 17 to to IN cord-001249-awn9ayy6 88 18 immunoextraction immunoextraction VB cord-001249-awn9ayy6 88 19 with with IN cord-001249-awn9ayy6 88 20 1 1 CD cord-001249-awn9ayy6 88 21 ml ml NNS cord-001249-awn9ayy6 88 22 of of IN cord-001249-awn9ayy6 88 23 an an DT cord-001249-awn9ayy6 88 24 appropriate appropriate JJ cord-001249-awn9ayy6 88 25 antibody antibody NN cord-001249-awn9ayy6 88 26 and and CC cord-001249-awn9ayy6 88 27 using use VBG cord-001249-awn9ayy6 88 28 protein protein NN cord-001249-awn9ayy6 88 29 G g NN cord-001249-awn9ayy6 88 30 - - HYPH cord-001249-awn9ayy6 88 31 agarose agarose VBN cord-001249-awn9ayy6 88 32 beads bead NNS cord-001249-awn9ayy6 88 33 ( ( -LRB- cord-001249-awn9ayy6 88 34 Millipore Millipore NNP cord-001249-awn9ayy6 88 35 ) ) -RRB- cord-001249-awn9ayy6 88 36 . . . cord-001249-awn9ayy6 89 1 After after IN cord-001249-awn9ayy6 89 2 a a DT cord-001249-awn9ayy6 89 3 series series NN cord-001249-awn9ayy6 89 4 of of IN cord-001249-awn9ayy6 89 5 washes wash NNS cord-001249-awn9ayy6 89 6 ( ( -LRB- cord-001249-awn9ayy6 89 7 2x 2x CD cord-001249-awn9ayy6 89 8 with with IN cord-001249-awn9ayy6 89 9 0.2 0.2 CD cord-001249-awn9ayy6 89 10 % % NN cord-001249-awn9ayy6 90 1 NP40 NP40 NNP cord-001249-awn9ayy6 90 2 , , , cord-001249-awn9ayy6 90 3 10 10 CD cord-001249-awn9ayy6 90 4 mM mM NNP cord-001249-awn9ayy6 90 5 Tris Tris NNP cord-001249-awn9ayy6 90 6 / / SYM cord-001249-awn9ayy6 90 7 Cl Cl NNP cord-001249-awn9ayy6 90 8 pH pH NNP cord-001249-awn9ayy6 90 9 7.5 7.5 CD cord-001249-awn9ayy6 90 10 , , , cord-001249-awn9ayy6 90 11 150 150 CD cord-001249-awn9ayy6 90 12 mM mm CD cord-001249-awn9ayy6 90 13 NaCl nacl NN cord-001249-awn9ayy6 90 14 , , , cord-001249-awn9ayy6 90 15 2 2 CD cord-001249-awn9ayy6 90 16 mM mm NN cord-001249-awn9ayy6 90 17 EDTA edta NN cord-001249-awn9ayy6 90 18 ; ; : cord-001249-awn9ayy6 91 1 2x 2x CD cord-001249-awn9ayy6 91 2 with with IN cord-001249-awn9ayy6 91 3 0.2 0.2 CD cord-001249-awn9ayy6 91 4 % % NN cord-001249-awn9ayy6 92 1 NP40 NP40 NNP cord-001249-awn9ayy6 92 2 , , , cord-001249-awn9ayy6 92 3 10 10 CD cord-001249-awn9ayy6 92 4 mM mM NNP cord-001249-awn9ayy6 92 5 Tris Tris NNP cord-001249-awn9ayy6 92 6 / / SYM cord-001249-awn9ayy6 92 7 Cl Cl NNP cord-001249-awn9ayy6 92 8 pH pH NNP cord-001249-awn9ayy6 92 9 7.5 7.5 CD cord-001249-awn9ayy6 92 10 , , , cord-001249-awn9ayy6 92 11 500 500 CD cord-001249-awn9ayy6 92 12 mM mm CD cord-001249-awn9ayy6 92 13 NaCl nacl NN cord-001249-awn9ayy6 92 14 , , , cord-001249-awn9ayy6 92 15 2 2 CD cord-001249-awn9ayy6 92 16 mM mm NN cord-001249-awn9ayy6 92 17 EDTA edta NN cord-001249-awn9ayy6 92 18 ; ; : cord-001249-awn9ayy6 92 19 and and CC cord-001249-awn9ayy6 92 20 1x 1x CD cord-001249-awn9ayy6 92 21 with with IN cord-001249-awn9ayy6 92 22 10 10 CD cord-001249-awn9ayy6 92 23 mM mM NNP cord-001249-awn9ayy6 92 24 Tris Tris NNP cord-001249-awn9ayy6 92 25 / / SYM cord-001249-awn9ayy6 92 26 Cl Cl NNP cord-001249-awn9ayy6 92 27 pH pH NNP cord-001249-awn9ayy6 92 28 7.5 7.5 CD cord-001249-awn9ayy6 92 29 ) ) -RRB- cord-001249-awn9ayy6 93 1 , , , cord-001249-awn9ayy6 93 2 the the DT cord-001249-awn9ayy6 93 3 immunoprecipitated immunoprecipitated JJ cord-001249-awn9ayy6 93 4 proteins protein NNS cord-001249-awn9ayy6 93 5 were be VBD cord-001249-awn9ayy6 93 6 analysed analyse VBN cord-001249-awn9ayy6 93 7 by by IN cord-001249-awn9ayy6 93 8 sodium sodium NN cord-001249-awn9ayy6 93 9 dodecyl dodecyl NNP cord-001249-awn9ayy6 93 10 sulphate sulphate NN cord-001249-awn9ayy6 93 11 polyacrylamide polyacrylamide NN cord-001249-awn9ayy6 93 12 gel gel NN cord-001249-awn9ayy6 93 13 electrophoresis electrophoresis NN cord-001249-awn9ayy6 93 14 ( ( -LRB- cord-001249-awn9ayy6 93 15 SDS SDS NNP cord-001249-awn9ayy6 93 16 - - HYPH cord-001249-awn9ayy6 93 17 PAGE PAGE NNP cord-001249-awn9ayy6 93 18 ) ) -RRB- cord-001249-awn9ayy6 93 19 and and CC cord-001249-awn9ayy6 93 20 immunoblotting immunoblotte VBG cord-001249-awn9ayy6 93 21 using use VBG cord-001249-awn9ayy6 93 22 specific specific JJ cord-001249-awn9ayy6 93 23 antibodies antibody NNS cord-001249-awn9ayy6 93 24 . . . cord-001249-awn9ayy6 94 1 At at IN cord-001249-awn9ayy6 94 2 the the DT cord-001249-awn9ayy6 94 3 indicated indicate VBN cord-001249-awn9ayy6 94 4 times time NNS cord-001249-awn9ayy6 94 5 , , , cord-001249-awn9ayy6 94 6 infected infected JJ cord-001249-awn9ayy6 94 7 or or CC cord-001249-awn9ayy6 94 8 transfected transfecte VBN cord-001249-awn9ayy6 94 9 cells cell NNS cord-001249-awn9ayy6 94 10 were be VBD cord-001249-awn9ayy6 94 11 harvested harvest VBN cord-001249-awn9ayy6 94 12 and and CC cord-001249-awn9ayy6 94 13 lysed lyse VBN cord-001249-awn9ayy6 94 14 with with IN cord-001249-awn9ayy6 94 15 100 100 CD cord-001249-awn9ayy6 94 16 ml ml NNS cord-001249-awn9ayy6 94 17 of of IN cord-001249-awn9ayy6 94 18 1x 1x CD cord-001249-awn9ayy6 94 19 SDS SDS NNP cord-001249-awn9ayy6 94 20 sample sample NN cord-001249-awn9ayy6 94 21 buffer buffer NN cord-001249-awn9ayy6 94 22 ( ( -LRB- cord-001249-awn9ayy6 94 23 New New NNP cord-001249-awn9ayy6 94 24 England England NNP cord-001249-awn9ayy6 94 25 Biolabs Biolabs NNP cord-001249-awn9ayy6 94 26 ) ) -RRB- cord-001249-awn9ayy6 94 27 . . . cord-001249-awn9ayy6 95 1 SDS SDS NNP cord-001249-awn9ayy6 95 2 - - HYPH cord-001249-awn9ayy6 95 3 PAGE PAGE NNP cord-001249-awn9ayy6 95 4 and and CC cord-001249-awn9ayy6 95 5 Western western JJ cord-001249-awn9ayy6 95 6 blots blot NNS cord-001249-awn9ayy6 95 7 were be VBD cord-001249-awn9ayy6 95 8 carried carry VBN cord-001249-awn9ayy6 95 9 out out RP cord-001249-awn9ayy6 95 10 as as IN cord-001249-awn9ayy6 95 11 previously previously RB cord-001249-awn9ayy6 95 12 described describe VBN cord-001249-awn9ayy6 95 13 [ [ -LRB- cord-001249-awn9ayy6 95 14 65 65 CD cord-001249-awn9ayy6 95 15 ] ] -RRB- cord-001249-awn9ayy6 95 16 . . . cord-001249-awn9ayy6 96 1 To to TO cord-001249-awn9ayy6 96 2 analyse analyse VB cord-001249-awn9ayy6 96 3 secreted secrete VBN cord-001249-awn9ayy6 96 4 proteins protein NNS cord-001249-awn9ayy6 96 5 , , , cord-001249-awn9ayy6 96 6 cell cell NN cord-001249-awn9ayy6 96 7 were be VBD cord-001249-awn9ayy6 96 8 cultured culture VBN cord-001249-awn9ayy6 96 9 in in IN cord-001249-awn9ayy6 96 10 12-well 12-well CD cord-001249-awn9ayy6 96 11 plates plate NNS cord-001249-awn9ayy6 96 12 containing contain VBG cord-001249-awn9ayy6 96 13 0.5 0.5 CD cord-001249-awn9ayy6 96 14 ml ml NNS cord-001249-awn9ayy6 96 15 of of IN cord-001249-awn9ayy6 96 16 growth growth NN cord-001249-awn9ayy6 96 17 medium medium NN cord-001249-awn9ayy6 96 18 per per IN cord-001249-awn9ayy6 96 19 well well RB cord-001249-awn9ayy6 96 20 . . . cord-001249-awn9ayy6 97 1 The the DT cord-001249-awn9ayy6 97 2 medium medium NN cord-001249-awn9ayy6 97 3 was be VBD cord-001249-awn9ayy6 97 4 collected collect VBN cord-001249-awn9ayy6 97 5 , , , cord-001249-awn9ayy6 97 6 pre pre VBN cord-001249-awn9ayy6 97 7 - - VBN cord-001249-awn9ayy6 97 8 cleared clear VBN cord-001249-awn9ayy6 97 9 of of IN cord-001249-awn9ayy6 97 10 cellular cellular JJ cord-001249-awn9ayy6 97 11 debris debris NN cord-001249-awn9ayy6 97 12 by by IN cord-001249-awn9ayy6 97 13 centrifugation centrifugation NN cord-001249-awn9ayy6 97 14 at at IN cord-001249-awn9ayy6 97 15 664 664 CD cord-001249-awn9ayy6 97 16 g g NN cord-001249-awn9ayy6 97 17 for for IN cord-001249-awn9ayy6 97 18 10 10 CD cord-001249-awn9ayy6 97 19 min min NN cord-001249-awn9ayy6 97 20 at at IN cord-001249-awn9ayy6 97 21 4uC 4uc CD cord-001249-awn9ayy6 97 22 , , , cord-001249-awn9ayy6 97 23 and and CC cord-001249-awn9ayy6 97 24 supernatants supernatant NNS cord-001249-awn9ayy6 97 25 were be VBD cord-001249-awn9ayy6 97 26 mixed mix VBN cord-001249-awn9ayy6 97 27 with with IN cord-001249-awn9ayy6 97 28 5x 5x CD cord-001249-awn9ayy6 97 29 SDS SDS NNP cord-001249-awn9ayy6 97 30 - - HYPH cord-001249-awn9ayy6 97 31 PAGE page NN cord-001249-awn9ayy6 97 32 loading loading NN cord-001249-awn9ayy6 97 33 buffer buffer NN cord-001249-awn9ayy6 97 34 ( ( -LRB- cord-001249-awn9ayy6 97 35 4 4 CD cord-001249-awn9ayy6 97 36 % % NN cord-001249-awn9ayy6 97 37 SDS SDS NNP cord-001249-awn9ayy6 97 38 , , , cord-001249-awn9ayy6 97 39 250 250 CD cord-001249-awn9ayy6 97 40 mM mm CD cord-001249-awn9ayy6 97 41 Tris/ Tris/ NNP cord-001249-awn9ayy6 98 1 Cl Cl NNP cord-001249-awn9ayy6 98 2 pH pH NNP cord-001249-awn9ayy6 98 3 6.8 6.8 CD cord-001249-awn9ayy6 98 4 , , , cord-001249-awn9ayy6 98 5 0.6 0.6 CD cord-001249-awn9ayy6 98 6 mg mg NN cord-001249-awn9ayy6 98 7 / / SYM cord-001249-awn9ayy6 98 8 ml ml NNS cord-001249-awn9ayy6 98 9 of of IN cord-001249-awn9ayy6 98 10 bromophenol bromophenol NNP cord-001249-awn9ayy6 98 11 blue blue NNP cord-001249-awn9ayy6 98 12 , , , cord-001249-awn9ayy6 98 13 7.5 7.5 CD cord-001249-awn9ayy6 98 14 mM mm CD cord-001249-awn9ayy6 98 15 DTT DTT NNP cord-001249-awn9ayy6 98 16 , , , cord-001249-awn9ayy6 98 17 30 30 CD cord-001249-awn9ayy6 98 18 % % NN cord-001249-awn9ayy6 98 19 ( ( -LRB- cord-001249-awn9ayy6 98 20 v v NN cord-001249-awn9ayy6 98 21 / / SYM cord-001249-awn9ayy6 98 22 v v NN cord-001249-awn9ayy6 98 23 ) ) -RRB- cord-001249-awn9ayy6 98 24 glycerol glycerol NN cord-001249-awn9ayy6 98 25 in in IN cord-001249-awn9ayy6 98 26 water water NN cord-001249-awn9ayy6 98 27 ) ) -RRB- cord-001249-awn9ayy6 98 28 . . . cord-001249-awn9ayy6 99 1 Such such JJ cord-001249-awn9ayy6 99 2 prepared prepared JJ cord-001249-awn9ayy6 99 3 protein protein NN cord-001249-awn9ayy6 99 4 lysates lysate NNS cord-001249-awn9ayy6 99 5 were be VBD cord-001249-awn9ayy6 99 6 analysed analyse VBN cord-001249-awn9ayy6 99 7 by by IN cord-001249-awn9ayy6 99 8 Western western JJ cord-001249-awn9ayy6 99 9 blotting blotting NN cord-001249-awn9ayy6 99 10 as as IN cord-001249-awn9ayy6 99 11 described describe VBN cord-001249-awn9ayy6 99 12 above above RB cord-001249-awn9ayy6 99 13 . . . cord-001249-awn9ayy6 100 1 ERp57 ERp57 NNS cord-001249-awn9ayy6 100 2 while while IN cord-001249-awn9ayy6 100 3 the the DT cord-001249-awn9ayy6 100 4 structure structure NN cord-001249-awn9ayy6 100 5 of of IN cord-001249-awn9ayy6 100 6 the the DT cord-001249-awn9ayy6 100 7 ER ER NNP cord-001249-awn9ayy6 100 8 , , , cord-001249-awn9ayy6 100 9 ERGIC ERGIC NNP cord-001249-awn9ayy6 100 10 and and CC cord-001249-awn9ayy6 100 11 Golgi Golgi NNP cord-001249-awn9ayy6 100 12 remains remain VBZ cord-001249-awn9ayy6 100 13 unchanged unchanged JJ cord-001249-awn9ayy6 101 1 Two two CD cord-001249-awn9ayy6 101 2 isolates isolate NNS cord-001249-awn9ayy6 101 3 of of IN cord-001249-awn9ayy6 101 4 NSDV NSDV NNP cord-001249-awn9ayy6 101 5 have have VBP cord-001249-awn9ayy6 101 6 been be VBN cord-001249-awn9ayy6 101 7 previously previously RB cord-001249-awn9ayy6 101 8 described describe VBN cord-001249-awn9ayy6 101 9 by by IN cord-001249-awn9ayy6 101 10 us -PRON- PRP cord-001249-awn9ayy6 101 11 [ [ -LRB- cord-001249-awn9ayy6 101 12 29 29 CD cord-001249-awn9ayy6 101 13 ] ] -RRB- cord-001249-awn9ayy6 101 14 : : : cord-001249-awn9ayy6 101 15 a a DT cord-001249-awn9ayy6 101 16 multiple multiple JJ cord-001249-awn9ayy6 101 17 - - HYPH cord-001249-awn9ayy6 101 18 times time NNS cord-001249-awn9ayy6 101 19 passaged passage VBN cord-001249-awn9ayy6 101 20 isolate isolate NN cord-001249-awn9ayy6 101 21 of of IN cord-001249-awn9ayy6 101 22 NSDV NSDV NNP cord-001249-awn9ayy6 101 23 from from IN cord-001249-awn9ayy6 101 24 Uganda Uganda NNP cord-001249-awn9ayy6 101 25 , , , cord-001249-awn9ayy6 101 26 which which WDT cord-001249-awn9ayy6 101 27 appeared appear VBD cord-001249-awn9ayy6 101 28 attenuated attenuated JJ cord-001249-awn9ayy6 101 29 upon upon IN cord-001249-awn9ayy6 101 30 infection infection NN cord-001249-awn9ayy6 101 31 of of IN cord-001249-awn9ayy6 101 32 a a DT cord-001249-awn9ayy6 101 33 susceptible susceptible JJ cord-001249-awn9ayy6 101 34 animal animal NN cord-001249-awn9ayy6 101 35 , , , cord-001249-awn9ayy6 101 36 and and CC cord-001249-awn9ayy6 101 37 an an DT cord-001249-awn9ayy6 101 38 isolate isolate NN cord-001249-awn9ayy6 101 39 of of IN cord-001249-awn9ayy6 101 40 GV GV NNP cord-001249-awn9ayy6 101 41 from from IN cord-001249-awn9ayy6 101 42 India India NNP cord-001249-awn9ayy6 101 43 which which WDT cord-001249-awn9ayy6 101 44 had have VBD cord-001249-awn9ayy6 101 45 been be VBN cord-001249-awn9ayy6 101 46 passaged passage VBN cord-001249-awn9ayy6 101 47 a a DT cord-001249-awn9ayy6 101 48 limited limited JJ cord-001249-awn9ayy6 101 49 number number NN cord-001249-awn9ayy6 101 50 of of IN cord-001249-awn9ayy6 101 51 times time NNS cord-001249-awn9ayy6 101 52 in in IN cord-001249-awn9ayy6 101 53 mouse mouse NN cord-001249-awn9ayy6 101 54 brain brain NN cord-001249-awn9ayy6 101 55 or or CC cord-001249-awn9ayy6 101 56 BHK21 BHK21 NNP cord-001249-awn9ayy6 101 57 clone clone VBD cord-001249-awn9ayy6 101 58 13 13 CD cord-001249-awn9ayy6 101 59 cells cell NNS cord-001249-awn9ayy6 101 60 and and CC cord-001249-awn9ayy6 101 61 which which WDT cord-001249-awn9ayy6 101 62 caused cause VBD cord-001249-awn9ayy6 101 63 haemorrhagic haemorrhagic JJ cord-001249-awn9ayy6 101 64 gastroenteritis gastroenteritis NN cord-001249-awn9ayy6 101 65 in in IN cord-001249-awn9ayy6 101 66 sheep sheep NN cord-001249-awn9ayy6 101 67 upon upon IN cord-001249-awn9ayy6 101 68 experimental experimental JJ cord-001249-awn9ayy6 101 69 inoculation inoculation NN cord-001249-awn9ayy6 101 70 . . . cord-001249-awn9ayy6 102 1 Since since IN cord-001249-awn9ayy6 102 2 NSDV NSDV NNP cord-001249-awn9ayy6 102 3 and and CC cord-001249-awn9ayy6 102 4 GV GV NNP cord-001249-awn9ayy6 102 5 have have VBP cord-001249-awn9ayy6 102 6 been be VBN cord-001249-awn9ayy6 102 7 shown show VBN cord-001249-awn9ayy6 102 8 to to TO cord-001249-awn9ayy6 102 9 be be VB cord-001249-awn9ayy6 102 10 the the DT cord-001249-awn9ayy6 102 11 same same JJ cord-001249-awn9ayy6 102 12 virus virus NN cord-001249-awn9ayy6 102 13 [ [ -LRB- cord-001249-awn9ayy6 102 14 9 9 CD cord-001249-awn9ayy6 102 15 , , , cord-001249-awn9ayy6 102 16 10 10 CD cord-001249-awn9ayy6 102 17 ] ] -RRB- cord-001249-awn9ayy6 102 18 , , , cord-001249-awn9ayy6 102 19 for for IN cord-001249-awn9ayy6 102 20 simplicity simplicity NN cord-001249-awn9ayy6 102 21 we -PRON- PRP cord-001249-awn9ayy6 102 22 have have VBP cord-001249-awn9ayy6 102 23 referred refer VBN cord-001249-awn9ayy6 102 24 to to IN cord-001249-awn9ayy6 102 25 them -PRON- PRP cord-001249-awn9ayy6 102 26 both both DT cord-001249-awn9ayy6 102 27 as as IN cord-001249-awn9ayy6 102 28 NSDV NSDV NNP cord-001249-awn9ayy6 102 29 in in IN cord-001249-awn9ayy6 102 30 this this DT cord-001249-awn9ayy6 102 31 paper paper NN cord-001249-awn9ayy6 102 32 , , , cord-001249-awn9ayy6 102 33 NSDVi NSDVi NNS cord-001249-awn9ayy6 102 34 for for IN cord-001249-awn9ayy6 102 35 the the DT cord-001249-awn9ayy6 102 36 pathogenic pathogenic JJ cord-001249-awn9ayy6 102 37 and and CC cord-001249-awn9ayy6 102 38 NSDVu NSDVu NNPS cord-001249-awn9ayy6 102 39 for for IN cord-001249-awn9ayy6 102 40 the the DT cord-001249-awn9ayy6 102 41 apathogenic apathogenic NNP cord-001249-awn9ayy6 102 42 isolate isolate VB cord-001249-awn9ayy6 102 43 . . . cord-001249-awn9ayy6 103 1 We -PRON- PRP cord-001249-awn9ayy6 103 2 focussed focusse VBD cord-001249-awn9ayy6 103 3 on on IN cord-001249-awn9ayy6 103 4 the the DT cord-001249-awn9ayy6 103 5 pathogenic pathogenic JJ cord-001249-awn9ayy6 103 6 isolate isolate NN cord-001249-awn9ayy6 103 7 as as IN cord-001249-awn9ayy6 103 8 the the DT cord-001249-awn9ayy6 103 9 best good JJS cord-001249-awn9ayy6 103 10 representative representative NN cord-001249-awn9ayy6 103 11 of of IN cord-001249-awn9ayy6 103 12 a a DT cord-001249-awn9ayy6 103 13 wild wild JJ cord-001249-awn9ayy6 103 14 - - HYPH cord-001249-awn9ayy6 103 15 type type NN cord-001249-awn9ayy6 103 16 virus virus NN cord-001249-awn9ayy6 103 17 ; ; : cord-001249-awn9ayy6 103 18 this this DT cord-001249-awn9ayy6 103 19 virus virus NN cord-001249-awn9ayy6 103 20 replicated replicate VBD cord-001249-awn9ayy6 103 21 reasonably reasonably RB cord-001249-awn9ayy6 103 22 well well RB cord-001249-awn9ayy6 103 23 in in IN cord-001249-awn9ayy6 103 24 Vero Vero NNP cord-001249-awn9ayy6 103 25 cells cell NNS cord-001249-awn9ayy6 103 26 , , , cord-001249-awn9ayy6 103 27 although although IN cord-001249-awn9ayy6 103 28 it -PRON- PRP cord-001249-awn9ayy6 103 29 grew grow VBD cord-001249-awn9ayy6 103 30 poorly poorly RB cord-001249-awn9ayy6 103 31 in in IN cord-001249-awn9ayy6 103 32 most most JJS cord-001249-awn9ayy6 103 33 of of IN cord-001249-awn9ayy6 103 34 the the DT cord-001249-awn9ayy6 103 35 other other JJ cord-001249-awn9ayy6 103 36 cell cell NN cord-001249-awn9ayy6 103 37 lines line NNS cord-001249-awn9ayy6 103 38 tested test VBD cord-001249-awn9ayy6 103 39 [ [ -LRB- cord-001249-awn9ayy6 103 40 29 29 CD cord-001249-awn9ayy6 103 41 ] ] -RRB- cord-001249-awn9ayy6 103 42 . . . cord-001249-awn9ayy6 104 1 Vero Vero NNP cord-001249-awn9ayy6 104 2 cells cell NNS cord-001249-awn9ayy6 104 3 were be VBD cord-001249-awn9ayy6 104 4 infected infect VBN cord-001249-awn9ayy6 104 5 with with IN cord-001249-awn9ayy6 104 6 NSDVi NSDVi NNS cord-001249-awn9ayy6 104 7 at at IN cord-001249-awn9ayy6 104 8 a a DT cord-001249-awn9ayy6 104 9 low low JJ cord-001249-awn9ayy6 104 10 multiplicity multiplicity NN cord-001249-awn9ayy6 104 11 of of IN cord-001249-awn9ayy6 104 12 infection infection NN cord-001249-awn9ayy6 104 13 ( ( -LRB- cord-001249-awn9ayy6 104 14 MOI MOI NNP cord-001249-awn9ayy6 104 15 ) ) -RRB- cord-001249-awn9ayy6 104 16 , , , cord-001249-awn9ayy6 104 17 which which WDT cord-001249-awn9ayy6 104 18 allowed allow VBD cord-001249-awn9ayy6 104 19 for for IN cord-001249-awn9ayy6 104 20 direct direct JJ cord-001249-awn9ayy6 104 21 comparison comparison NN cord-001249-awn9ayy6 104 22 of of IN cord-001249-awn9ayy6 104 23 infected infected JJ cord-001249-awn9ayy6 104 24 and and CC cord-001249-awn9ayy6 104 25 uninfected uninfected JJ cord-001249-awn9ayy6 104 26 cells cell NNS cord-001249-awn9ayy6 104 27 on on IN cord-001249-awn9ayy6 104 28 a a DT cord-001249-awn9ayy6 104 29 single single JJ cord-001249-awn9ayy6 104 30 slide slide NN cord-001249-awn9ayy6 104 31 and and CC cord-001249-awn9ayy6 104 32 even even RB cord-001249-awn9ayy6 104 33 in in IN cord-001249-awn9ayy6 104 34 one one CD cord-001249-awn9ayy6 104 35 focal focal JJ cord-001249-awn9ayy6 104 36 view view NN cord-001249-awn9ayy6 104 37 . . . cord-001249-awn9ayy6 105 1 After after IN cord-001249-awn9ayy6 105 2 overnight overnight JJ cord-001249-awn9ayy6 105 3 infection infection NN cord-001249-awn9ayy6 105 4 , , , cord-001249-awn9ayy6 105 5 cells cell NNS cord-001249-awn9ayy6 105 6 were be VBD cord-001249-awn9ayy6 105 7 fixed fix VBN cord-001249-awn9ayy6 105 8 and and CC cord-001249-awn9ayy6 105 9 stained stain VBN cord-001249-awn9ayy6 105 10 with with IN cord-001249-awn9ayy6 105 11 antibodies antibody NNS cord-001249-awn9ayy6 105 12 specific specific JJ cord-001249-awn9ayy6 105 13 for for IN cord-001249-awn9ayy6 105 14 ERGIC ERGIC NNP cord-001249-awn9ayy6 105 15 ( ( -LRB- cord-001249-awn9ayy6 105 16 ERGIC53 ERGIC53 NNP cord-001249-awn9ayy6 105 17 ) ) -RRB- cord-001249-awn9ayy6 105 18 , , , cord-001249-awn9ayy6 105 19 cisGolgi cisGolgi NNP cord-001249-awn9ayy6 105 20 ( ( -LRB- cord-001249-awn9ayy6 105 21 GM130 GM130 NNP cord-001249-awn9ayy6 105 22 ) ) -RRB- cord-001249-awn9ayy6 105 23 or or CC cord-001249-awn9ayy6 105 24 transGolgi transGolgi NNP cord-001249-awn9ayy6 105 25 ( ( -LRB- cord-001249-awn9ayy6 105 26 p102 p102 NNP cord-001249-awn9ayy6 105 27 ) ) -RRB- cord-001249-awn9ayy6 105 28 markers marker NNS cord-001249-awn9ayy6 105 29 . . . cord-001249-awn9ayy6 106 1 Infected infected JJ cord-001249-awn9ayy6 106 2 cells cell NNS cord-001249-awn9ayy6 106 3 were be VBD cord-001249-awn9ayy6 106 4 detected detect VBN cord-001249-awn9ayy6 106 5 using use VBG cord-001249-awn9ayy6 106 6 rabbit rabbit NN cord-001249-awn9ayy6 106 7 polyclonal polyclonal JJ cord-001249-awn9ayy6 106 8 antiserum antiserum NN cord-001249-awn9ayy6 106 9 against against IN cord-001249-awn9ayy6 106 10 the the DT cord-001249-awn9ayy6 106 11 viral viral JJ cord-001249-awn9ayy6 106 12 N n CD cord-001249-awn9ayy6 106 13 protein protein NN cord-001249-awn9ayy6 106 14 ( ( -LRB- cord-001249-awn9ayy6 106 15 which which WDT cord-001249-awn9ayy6 106 16 recognises recognise VBZ cord-001249-awn9ayy6 106 17 both both DT cord-001249-awn9ayy6 106 18 NSDVi NSDVi NNS cord-001249-awn9ayy6 106 19 and and CC cord-001249-awn9ayy6 106 20 NSDVu NSDVu NNP cord-001249-awn9ayy6 106 21 isolates isolate VBZ cord-001249-awn9ayy6 106 22 ) ) -RRB- cord-001249-awn9ayy6 106 23 , , , cord-001249-awn9ayy6 106 24 and and CC cord-001249-awn9ayy6 106 25 the the DT cord-001249-awn9ayy6 106 26 distribution distribution NN cord-001249-awn9ayy6 106 27 of of IN cord-001249-awn9ayy6 106 28 the the DT cord-001249-awn9ayy6 106 29 cellular cellular JJ cord-001249-awn9ayy6 106 30 proteins protein NNS cord-001249-awn9ayy6 106 31 was be VBD cord-001249-awn9ayy6 106 32 compared compare VBN cord-001249-awn9ayy6 106 33 in in IN cord-001249-awn9ayy6 106 34 infected infected JJ cord-001249-awn9ayy6 106 35 and and CC cord-001249-awn9ayy6 106 36 uninfected uninfected JJ cord-001249-awn9ayy6 106 37 cells cell NNS cord-001249-awn9ayy6 106 38 by by IN cord-001249-awn9ayy6 106 39 confocal confocal JJ cord-001249-awn9ayy6 106 40 microscopy microscopy NNS cord-001249-awn9ayy6 106 41 . . . cord-001249-awn9ayy6 107 1 Apart apart RB cord-001249-awn9ayy6 107 2 from from IN cord-001249-awn9ayy6 107 3 a a DT cord-001249-awn9ayy6 107 4 few few JJ cord-001249-awn9ayy6 107 5 infected infected JJ cord-001249-awn9ayy6 107 6 cells cell NNS cord-001249-awn9ayy6 107 7 where where WRB cord-001249-awn9ayy6 107 8 the the DT cord-001249-awn9ayy6 107 9 N N NNP cord-001249-awn9ayy6 107 10 protein protein NN cord-001249-awn9ayy6 107 11 appeared appear VBD cord-001249-awn9ayy6 107 12 to to TO cord-001249-awn9ayy6 107 13 fill fill VB cord-001249-awn9ayy6 107 14 up up RP cord-001249-awn9ayy6 107 15 the the DT cord-001249-awn9ayy6 107 16 space space NN cord-001249-awn9ayy6 107 17 occupied occupy VBN cord-001249-awn9ayy6 107 18 by by IN cord-001249-awn9ayy6 107 19 the the DT cord-001249-awn9ayy6 107 20 ERGIC ERGIC NNP cord-001249-awn9ayy6 107 21 , , , cord-001249-awn9ayy6 107 22 no no DT cord-001249-awn9ayy6 107 23 obvious obvious JJ cord-001249-awn9ayy6 107 24 changes change NNS cord-001249-awn9ayy6 107 25 to to IN cord-001249-awn9ayy6 107 26 the the DT cord-001249-awn9ayy6 107 27 distribution distribution NN cord-001249-awn9ayy6 107 28 or or CC cord-001249-awn9ayy6 107 29 amount amount NN cord-001249-awn9ayy6 107 30 of of IN cord-001249-awn9ayy6 107 31 ERGIC53 ERGIC53 NNP cord-001249-awn9ayy6 107 32 was be VBD cord-001249-awn9ayy6 107 33 observed observe VBN cord-001249-awn9ayy6 108 1 ( ( -LRB- cord-001249-awn9ayy6 108 2 Figure figure NN cord-001249-awn9ayy6 108 3 1 1 CD cord-001249-awn9ayy6 108 4 .A .A . cord-001249-awn9ayy6 109 1 a a LS cord-001249-awn9ayy6 109 2 - - HYPH cord-001249-awn9ayy6 109 3 c c NN cord-001249-awn9ayy6 109 4 ) ) -RRB- cord-001249-awn9ayy6 109 5 . . . cord-001249-awn9ayy6 110 1 Similar similar JJ cord-001249-awn9ayy6 110 2 results result NNS cord-001249-awn9ayy6 110 3 were be VBD cord-001249-awn9ayy6 110 4 observed observe VBN cord-001249-awn9ayy6 110 5 for for IN cord-001249-awn9ayy6 110 6 the the DT cord-001249-awn9ayy6 110 7 cis cis NNP cord-001249-awn9ayy6 110 8 and and CC cord-001249-awn9ayy6 110 9 transGolgi transGolgi NNP cord-001249-awn9ayy6 110 10 compartments compartment NNS cord-001249-awn9ayy6 110 11 , , , cord-001249-awn9ayy6 110 12 with with IN cord-001249-awn9ayy6 110 13 no no DT cord-001249-awn9ayy6 110 14 obvious obvious JJ cord-001249-awn9ayy6 110 15 changes change NNS cord-001249-awn9ayy6 110 16 to to IN cord-001249-awn9ayy6 110 17 the the DT cord-001249-awn9ayy6 110 18 distribution distribution NN cord-001249-awn9ayy6 110 19 of of IN cord-001249-awn9ayy6 110 20 GM130 GM130 NNP cord-001249-awn9ayy6 110 21 and and CC cord-001249-awn9ayy6 110 22 p102 p102 NNP cord-001249-awn9ayy6 110 23 ( ( -LRB- cord-001249-awn9ayy6 110 24 which which WDT cord-001249-awn9ayy6 110 25 are be VBP cord-001249-awn9ayy6 110 26 matrix matrix NN cord-001249-awn9ayy6 110 27 proteins protein NNS cord-001249-awn9ayy6 110 28 of of IN cord-001249-awn9ayy6 110 29 the the DT cord-001249-awn9ayy6 110 30 cis cis NNP cord-001249-awn9ayy6 110 31 and and CC cord-001249-awn9ayy6 110 32 transGolgi transGolgi NNP cord-001249-awn9ayy6 110 33 respectively respectively RB cord-001249-awn9ayy6 110 34 [ [ -LRB- cord-001249-awn9ayy6 110 35 63 63 CD cord-001249-awn9ayy6 110 36 , , , cord-001249-awn9ayy6 110 37 66 66 CD cord-001249-awn9ayy6 110 38 ] ] -RRB- cord-001249-awn9ayy6 110 39 ) ) -RRB- cord-001249-awn9ayy6 110 40 when when WRB cord-001249-awn9ayy6 110 41 infected infected JJ cord-001249-awn9ayy6 110 42 cells cell NNS cord-001249-awn9ayy6 110 43 were be VBD cord-001249-awn9ayy6 110 44 compared compare VBN cord-001249-awn9ayy6 110 45 to to IN cord-001249-awn9ayy6 110 46 uninfected uninfected JJ cord-001249-awn9ayy6 110 47 cells cell NNS cord-001249-awn9ayy6 110 48 ( ( -LRB- cord-001249-awn9ayy6 111 1 Figure figure NN cord-001249-awn9ayy6 111 2 1 1 CD cord-001249-awn9ayy6 111 3 .A .A . cord-001249-awn9ayy6 112 1 d d LS cord-001249-awn9ayy6 112 2 - - : cord-001249-awn9ayy6 112 3 i i NNP cord-001249-awn9ayy6 112 4 ) ) -RRB- cord-001249-awn9ayy6 112 5 . . . cord-001249-awn9ayy6 113 1 NSDV NSDV NNP cord-001249-awn9ayy6 113 2 replication replication NN cord-001249-awn9ayy6 113 3 did do VBD cord-001249-awn9ayy6 113 4 not not RB cord-001249-awn9ayy6 113 5 appear appear VB cord-001249-awn9ayy6 113 6 to to TO cord-001249-awn9ayy6 113 7 affect affect VB cord-001249-awn9ayy6 113 8 protein protein NN cord-001249-awn9ayy6 113 9 levels level NNS cord-001249-awn9ayy6 113 10 of of IN cord-001249-awn9ayy6 113 11 these these DT cord-001249-awn9ayy6 113 12 markers marker NNS cord-001249-awn9ayy6 113 13 , , , cord-001249-awn9ayy6 113 14 as as IN cord-001249-awn9ayy6 113 15 judged judge VBN cord-001249-awn9ayy6 113 16 by by IN cord-001249-awn9ayy6 113 17 fluorescence fluorescence NN cord-001249-awn9ayy6 113 18 intensity intensity NN cord-001249-awn9ayy6 113 19 . . . cord-001249-awn9ayy6 114 1 The the DT cord-001249-awn9ayy6 114 2 same same JJ cord-001249-awn9ayy6 114 3 results result NNS cord-001249-awn9ayy6 114 4 were be VBD cord-001249-awn9ayy6 114 5 obtained obtain VBN cord-001249-awn9ayy6 114 6 for for IN cord-001249-awn9ayy6 114 7 NSDVi NSDVi NNS cord-001249-awn9ayy6 114 8 - - , cord-001249-awn9ayy6 114 9 infected infect VBN cord-001249-awn9ayy6 114 10 A549 a549 JJ cord-001249-awn9ayy6 114 11 cells cell NNS cord-001249-awn9ayy6 114 12 , , , cord-001249-awn9ayy6 114 13 and and CC cord-001249-awn9ayy6 114 14 for for IN cord-001249-awn9ayy6 114 15 Vero Vero NNP cord-001249-awn9ayy6 114 16 and and CC cord-001249-awn9ayy6 114 17 A549 a549 JJ cord-001249-awn9ayy6 114 18 cells cell NNS cord-001249-awn9ayy6 114 19 infected infect VBN cord-001249-awn9ayy6 114 20 with with IN cord-001249-awn9ayy6 114 21 NSDVu NSDVu NNS cord-001249-awn9ayy6 114 22 ( ( -LRB- cord-001249-awn9ayy6 114 23 data datum NNS cord-001249-awn9ayy6 114 24 not not RB cord-001249-awn9ayy6 114 25 shown show VBN cord-001249-awn9ayy6 114 26 ) ) -RRB- cord-001249-awn9ayy6 114 27 . . . cord-001249-awn9ayy6 115 1 These these DT cord-001249-awn9ayy6 115 2 results result NNS cord-001249-awn9ayy6 115 3 indicate indicate VBP cord-001249-awn9ayy6 115 4 that that IN cord-001249-awn9ayy6 115 5 , , , cord-001249-awn9ayy6 115 6 despite despite IN cord-001249-awn9ayy6 115 7 budding bud VBG cord-001249-awn9ayy6 115 8 into into IN cord-001249-awn9ayy6 115 9 the the DT cord-001249-awn9ayy6 115 10 Golgi Golgi NNP cord-001249-awn9ayy6 115 11 , , , cord-001249-awn9ayy6 115 12 often often RB cord-001249-awn9ayy6 115 13 shedding shed VBG cord-001249-awn9ayy6 115 14 large large JJ cord-001249-awn9ayy6 115 15 numbers number NNS cord-001249-awn9ayy6 115 16 of of IN cord-001249-awn9ayy6 115 17 the the DT cord-001249-awn9ayy6 115 18 virus virus NN cord-001249-awn9ayy6 115 19 particles particle NNS cord-001249-awn9ayy6 115 20 [ [ -LRB- cord-001249-awn9ayy6 115 21 35 35 CD cord-001249-awn9ayy6 115 22 ] ] -RRB- cord-001249-awn9ayy6 115 23 , , , cord-001249-awn9ayy6 115 24 NSDV NSDV NNP cord-001249-awn9ayy6 115 25 does do VBZ cord-001249-awn9ayy6 115 26 not not RB cord-001249-awn9ayy6 115 27 appear appear VB cord-001249-awn9ayy6 115 28 to to TO cord-001249-awn9ayy6 115 29 alter alter VB cord-001249-awn9ayy6 115 30 ERGIC ERGIC NNP cord-001249-awn9ayy6 115 31 and and CC cord-001249-awn9ayy6 115 32 Golgi Golgi NNP cord-001249-awn9ayy6 115 33 structures structure NNS cord-001249-awn9ayy6 115 34 ( ( -LRB- cord-001249-awn9ayy6 115 35 at at IN cord-001249-awn9ayy6 115 36 least least JJS cord-001249-awn9ayy6 115 37 not not RB cord-001249-awn9ayy6 115 38 sufficiently sufficiently RB cord-001249-awn9ayy6 115 39 for for IN cord-001249-awn9ayy6 115 40 the the DT cord-001249-awn9ayy6 115 41 alteration alteration NN cord-001249-awn9ayy6 115 42 to to TO cord-001249-awn9ayy6 115 43 be be VB cord-001249-awn9ayy6 115 44 detected detect VBN cord-001249-awn9ayy6 115 45 by by IN cord-001249-awn9ayy6 115 46 confocal confocal JJ cord-001249-awn9ayy6 115 47 microscopy microscopy NNS cord-001249-awn9ayy6 115 48 ) ) -RRB- cord-001249-awn9ayy6 115 49 . . . cord-001249-awn9ayy6 116 1 This this DT cord-001249-awn9ayy6 116 2 suggests suggest VBZ cord-001249-awn9ayy6 116 3 that that IN cord-001249-awn9ayy6 116 4 the the DT cord-001249-awn9ayy6 116 5 Golgi Golgi NNP cord-001249-awn9ayy6 116 6 and and CC cord-001249-awn9ayy6 116 7 ERGIC ERGIC NNP cord-001249-awn9ayy6 116 8 remain remain VBP cord-001249-awn9ayy6 116 9 functional functional JJ cord-001249-awn9ayy6 116 10 during during IN cord-001249-awn9ayy6 116 11 infection infection NN cord-001249-awn9ayy6 116 12 , , , cord-001249-awn9ayy6 116 13 thereby thereby RB cord-001249-awn9ayy6 116 14 promoting promote VBG cord-001249-awn9ayy6 116 15 viral viral JJ cord-001249-awn9ayy6 116 16 egress egress NN cord-001249-awn9ayy6 116 17 . . . cord-001249-awn9ayy6 117 1 NSDV NSDV NNP cord-001249-awn9ayy6 117 2 replication replication NN cord-001249-awn9ayy6 117 3 also also RB cord-001249-awn9ayy6 117 4 had have VBD cord-001249-awn9ayy6 117 5 no no DT cord-001249-awn9ayy6 117 6 apparent apparent JJ cord-001249-awn9ayy6 117 7 effect effect NN cord-001249-awn9ayy6 117 8 on on IN cord-001249-awn9ayy6 117 9 microtubule microtubule NN cord-001249-awn9ayy6 117 10 and and CC cord-001249-awn9ayy6 117 11 actin actin NN cord-001249-awn9ayy6 117 12 filament filament NN cord-001249-awn9ayy6 117 13 organisation organisation NN cord-001249-awn9ayy6 117 14 ( ( -LRB- cord-001249-awn9ayy6 117 15 Figure Figure NNP cord-001249-awn9ayy6 117 16 S1 S1 NNP cord-001249-awn9ayy6 117 17 ) ) -RRB- cord-001249-awn9ayy6 117 18 ; ; : cord-001249-awn9ayy6 117 19 in in IN cord-001249-awn9ayy6 117 20 addition addition NN cord-001249-awn9ayy6 117 21 , , , cord-001249-awn9ayy6 117 22 several several JJ cord-001249-awn9ayy6 117 23 NSDVinfected nsdvinfected JJ cord-001249-awn9ayy6 117 24 cells cell NNS cord-001249-awn9ayy6 117 25 were be VBD cord-001249-awn9ayy6 117 26 found find VBN cord-001249-awn9ayy6 117 27 undergoing undergo VBG cord-001249-awn9ayy6 117 28 mitosis mitosis NN cord-001249-awn9ayy6 117 29 , , , cord-001249-awn9ayy6 117 30 showing show VBG cord-001249-awn9ayy6 117 31 a a DT cord-001249-awn9ayy6 117 32 welldefined welldefine VBN cord-001249-awn9ayy6 117 33 mitotic mitotic JJ cord-001249-awn9ayy6 117 34 spindle spindle NN cord-001249-awn9ayy6 117 35 as as IN cord-001249-awn9ayy6 117 36 observed observe VBN cord-001249-awn9ayy6 117 37 by by IN cord-001249-awn9ayy6 117 38 a a DT cord-001249-awn9ayy6 117 39 - - HYPH cord-001249-awn9ayy6 117 40 tubulin tubulin NN cord-001249-awn9ayy6 117 41 staining staining NN cord-001249-awn9ayy6 117 42 , , , cord-001249-awn9ayy6 117 43 indicating indicate VBG cord-001249-awn9ayy6 117 44 that that IN cord-001249-awn9ayy6 117 45 the the DT cord-001249-awn9ayy6 117 46 centrioles centriole NNS cord-001249-awn9ayy6 117 47 likewise likewise RB cord-001249-awn9ayy6 117 48 remained remain VBD cord-001249-awn9ayy6 117 49 functional functional JJ cord-001249-awn9ayy6 117 50 in in IN cord-001249-awn9ayy6 117 51 NSDV NSDV NNP cord-001249-awn9ayy6 117 52 - - HYPH cord-001249-awn9ayy6 117 53 infected infect VBN cord-001249-awn9ayy6 117 54 cells cell NNS cord-001249-awn9ayy6 117 55 ( ( -LRB- cord-001249-awn9ayy6 117 56 Figure Figure NNP cord-001249-awn9ayy6 117 57 S1 S1 NNP cord-001249-awn9ayy6 117 58 ) ) -RRB- cord-001249-awn9ayy6 117 59 . . . cord-001249-awn9ayy6 118 1 A a DT cord-001249-awn9ayy6 118 2 different different JJ cord-001249-awn9ayy6 118 3 picture picture NN cord-001249-awn9ayy6 118 4 was be VBD cord-001249-awn9ayy6 118 5 observed observe VBN cord-001249-awn9ayy6 118 6 when when WRB cord-001249-awn9ayy6 118 7 the the DT cord-001249-awn9ayy6 118 8 ER ER NNP cord-001249-awn9ayy6 118 9 of of IN cord-001249-awn9ayy6 118 10 infected infected JJ cord-001249-awn9ayy6 118 11 cells cell NNS cord-001249-awn9ayy6 118 12 was be VBD cord-001249-awn9ayy6 118 13 observed observe VBN cord-001249-awn9ayy6 118 14 . . . cord-001249-awn9ayy6 119 1 When when WRB cord-001249-awn9ayy6 119 2 staining staining NN cord-001249-awn9ayy6 119 3 cells cell NNS cord-001249-awn9ayy6 119 4 with with IN cord-001249-awn9ayy6 119 5 mouse mouse NN cord-001249-awn9ayy6 119 6 anti anti JJ cord-001249-awn9ayy6 119 7 - - JJ cord-001249-awn9ayy6 119 8 PDI pdi JJ cord-001249-awn9ayy6 119 9 antibody antibody NN cord-001249-awn9ayy6 119 10 clone clone NN cord-001249-awn9ayy6 119 11 1D3 1d3 CD cord-001249-awn9ayy6 119 12 , , , cord-001249-awn9ayy6 119 13 which which WDT cord-001249-awn9ayy6 119 14 targets target VBZ cord-001249-awn9ayy6 119 15 the the DT cord-001249-awn9ayy6 119 16 C C NNP cord-001249-awn9ayy6 119 17 - - HYPH cord-001249-awn9ayy6 119 18 terminal terminal NN cord-001249-awn9ayy6 119 19 part part NN cord-001249-awn9ayy6 119 20 of of IN cord-001249-awn9ayy6 119 21 PDI PDI NNP cord-001249-awn9ayy6 119 22 , , , cord-001249-awn9ayy6 119 23 containing contain VBG cord-001249-awn9ayy6 119 24 the the DT cord-001249-awn9ayy6 119 25 KDEL KDEL NNP cord-001249-awn9ayy6 119 26 retention retention NN cord-001249-awn9ayy6 119 27 motif motif NN cord-001249-awn9ayy6 119 28 [ [ -LRB- cord-001249-awn9ayy6 119 29 67 67 CD cord-001249-awn9ayy6 119 30 ] ] -RRB- cord-001249-awn9ayy6 119 31 , , , cord-001249-awn9ayy6 119 32 we -PRON- PRP cord-001249-awn9ayy6 119 33 discovered discover VBD cord-001249-awn9ayy6 119 34 that that IN cord-001249-awn9ayy6 119 35 in in IN cord-001249-awn9ayy6 119 36 many many JJ cord-001249-awn9ayy6 119 37 infected infected JJ cord-001249-awn9ayy6 119 38 cells cell NNS cord-001249-awn9ayy6 119 39 the the DT cord-001249-awn9ayy6 119 40 amount amount NN cord-001249-awn9ayy6 119 41 of of IN cord-001249-awn9ayy6 119 42 PDI PDI NNP cord-001249-awn9ayy6 119 43 appeared appear VBD cord-001249-awn9ayy6 119 44 to to TO cord-001249-awn9ayy6 119 45 decrease decrease VB cord-001249-awn9ayy6 119 46 , , , cord-001249-awn9ayy6 119 47 even even RB cord-001249-awn9ayy6 119 48 disappearing disappear VBG cord-001249-awn9ayy6 119 49 completely completely RB cord-001249-awn9ayy6 119 50 from from IN cord-001249-awn9ayy6 119 51 the the DT cord-001249-awn9ayy6 119 52 ER ER NNP cord-001249-awn9ayy6 119 53 of of IN cord-001249-awn9ayy6 119 54 about about RB cord-001249-awn9ayy6 119 55 1 1 CD cord-001249-awn9ayy6 119 56 in in IN cord-001249-awn9ayy6 119 57 3 3 CD cord-001249-awn9ayy6 119 58 infected infected JJ cord-001249-awn9ayy6 119 59 cells cell NNS cord-001249-awn9ayy6 119 60 ( ( -LRB- cord-001249-awn9ayy6 119 61 Figure figure NN cord-001249-awn9ayy6 119 62 2 2 CD cord-001249-awn9ayy6 119 63 a a DT cord-001249-awn9ayy6 119 64 - - HYPH cord-001249-awn9ayy6 119 65 c c NN cord-001249-awn9ayy6 119 66 ) ) -RRB- cord-001249-awn9ayy6 119 67 . . . cord-001249-awn9ayy6 120 1 To to TO cord-001249-awn9ayy6 120 2 exclude exclude VB cord-001249-awn9ayy6 120 3 the the DT cord-001249-awn9ayy6 120 4 possibility possibility NN cord-001249-awn9ayy6 120 5 that that IN cord-001249-awn9ayy6 120 6 the the DT cord-001249-awn9ayy6 120 7 viral viral JJ cord-001249-awn9ayy6 120 8 glycoproteins glycoprotein NNS cord-001249-awn9ayy6 120 9 mask mask NN cord-001249-awn9ayy6 121 1 the the DT cord-001249-awn9ayy6 121 2 epitope epitope NN cord-001249-awn9ayy6 121 3 recognised recognise VBN cord-001249-awn9ayy6 121 4 by by IN cord-001249-awn9ayy6 121 5 the the DT cord-001249-awn9ayy6 121 6 antibody antibody NN cord-001249-awn9ayy6 121 7 clone clone NN cord-001249-awn9ayy6 121 8 1D3 1d3 CD cord-001249-awn9ayy6 121 9 , , , cord-001249-awn9ayy6 121 10 the the DT cord-001249-awn9ayy6 121 11 experiment experiment NN cord-001249-awn9ayy6 121 12 was be VBD cord-001249-awn9ayy6 121 13 repeated repeat VBN cord-001249-awn9ayy6 121 14 using use VBG cord-001249-awn9ayy6 121 15 mouse mouse NN cord-001249-awn9ayy6 121 16 anti anti JJ cord-001249-awn9ayy6 121 17 - - JJ cord-001249-awn9ayy6 121 18 PDI pdi JJ cord-001249-awn9ayy6 121 19 antibody antibody NN cord-001249-awn9ayy6 121 20 clone clone NN cord-001249-awn9ayy6 121 21 RL90 RL90 NNP cord-001249-awn9ayy6 121 22 , , , cord-001249-awn9ayy6 121 23 which which WDT cord-001249-awn9ayy6 121 24 targets target VBZ cord-001249-awn9ayy6 121 25 a a DT cord-001249-awn9ayy6 121 26 different different JJ cord-001249-awn9ayy6 121 27 PDI PDI NNP cord-001249-awn9ayy6 121 28 epitope epitope NN cord-001249-awn9ayy6 121 29 from from IN cord-001249-awn9ayy6 121 30 that that DT cord-001249-awn9ayy6 121 31 recognised recognise VBN cord-001249-awn9ayy6 121 32 by by IN cord-001249-awn9ayy6 121 33 1D3 1d3 CD cord-001249-awn9ayy6 121 34 , , , cord-001249-awn9ayy6 121 35 since since IN cord-001249-awn9ayy6 121 36 it -PRON- PRP cord-001249-awn9ayy6 121 37 inhibits inhibit VBZ cord-001249-awn9ayy6 121 38 the the DT cord-001249-awn9ayy6 121 39 activity activity NN cord-001249-awn9ayy6 121 40 of of IN cord-001249-awn9ayy6 121 41 PDI PDI NNP cord-001249-awn9ayy6 121 42 in in IN cord-001249-awn9ayy6 121 43 vitro vitro FW cord-001249-awn9ayy6 121 44 [ [ -LRB- cord-001249-awn9ayy6 121 45 68 68 CD cord-001249-awn9ayy6 121 46 ] ] -RRB- cord-001249-awn9ayy6 121 47 and and CC cord-001249-awn9ayy6 121 48 therefore therefore RB cord-001249-awn9ayy6 121 49 recognises recognise VBZ cord-001249-awn9ayy6 121 50 a a DT cord-001249-awn9ayy6 121 51 region region NN cord-001249-awn9ayy6 121 52 close close JJ cord-001249-awn9ayy6 121 53 to to IN cord-001249-awn9ayy6 121 54 the the DT cord-001249-awn9ayy6 121 55 enzymatic enzymatic JJ cord-001249-awn9ayy6 121 56 site site NN cord-001249-awn9ayy6 121 57 of of IN cord-001249-awn9ayy6 121 58 the the DT cord-001249-awn9ayy6 121 59 protein protein NN cord-001249-awn9ayy6 121 60 . . . cord-001249-awn9ayy6 122 1 Once once RB cord-001249-awn9ayy6 122 2 again again RB cord-001249-awn9ayy6 122 3 , , , cord-001249-awn9ayy6 122 4 many many JJ cord-001249-awn9ayy6 122 5 infected infected JJ cord-001249-awn9ayy6 122 6 cells cell NNS cord-001249-awn9ayy6 122 7 showed show VBD cord-001249-awn9ayy6 122 8 a a DT cord-001249-awn9ayy6 122 9 reduced reduce VBN cord-001249-awn9ayy6 122 10 amount amount NN cord-001249-awn9ayy6 122 11 of of IN cord-001249-awn9ayy6 122 12 PDI PDI NNP cord-001249-awn9ayy6 122 13 in in IN cord-001249-awn9ayy6 122 14 the the DT cord-001249-awn9ayy6 122 15 ER er NN cord-001249-awn9ayy6 122 16 ( ( -LRB- cord-001249-awn9ayy6 122 17 Figure figure NN cord-001249-awn9ayy6 122 18 2 2 CD cord-001249-awn9ayy6 122 19 d d NN cord-001249-awn9ayy6 122 20 - - HYPH cord-001249-awn9ayy6 122 21 f f NNP cord-001249-awn9ayy6 122 22 ) ) -RRB- cord-001249-awn9ayy6 122 23 , , , cord-001249-awn9ayy6 122 24 confirming confirm VBG cord-001249-awn9ayy6 122 25 that that IN cord-001249-awn9ayy6 122 26 , , , cord-001249-awn9ayy6 122 27 in in IN cord-001249-awn9ayy6 122 28 NSDV NSDV NNP cord-001249-awn9ayy6 122 29 - - HYPH cord-001249-awn9ayy6 122 30 infected infect VBN cord-001249-awn9ayy6 122 31 cells cell NNS cord-001249-awn9ayy6 122 32 , , , cord-001249-awn9ayy6 122 33 PDI PDI NNP cord-001249-awn9ayy6 122 34 shows show VBZ cord-001249-awn9ayy6 122 35 a a DT cord-001249-awn9ayy6 122 36 different different JJ cord-001249-awn9ayy6 122 37 staining staining NN cord-001249-awn9ayy6 122 38 pattern pattern NN cord-001249-awn9ayy6 122 39 than than IN cord-001249-awn9ayy6 122 40 in in IN cord-001249-awn9ayy6 122 41 uninfected uninfected JJ cord-001249-awn9ayy6 122 42 cells cell NNS cord-001249-awn9ayy6 122 43 , , , cord-001249-awn9ayy6 122 44 and and CC cord-001249-awn9ayy6 122 45 that that IN cord-001249-awn9ayy6 122 46 many many JJ cord-001249-awn9ayy6 122 47 infected infected JJ cord-001249-awn9ayy6 122 48 cells cell NNS cord-001249-awn9ayy6 122 49 even even RB cord-001249-awn9ayy6 122 50 appear appear VBP cord-001249-awn9ayy6 122 51 to to TO cord-001249-awn9ayy6 122 52 lose lose VB cord-001249-awn9ayy6 122 53 PDI PDI NNP cord-001249-awn9ayy6 122 54 entirely entirely RB cord-001249-awn9ayy6 122 55 . . . cord-001249-awn9ayy6 123 1 The the DT cord-001249-awn9ayy6 123 2 distribution distribution NN cord-001249-awn9ayy6 123 3 of of IN cord-001249-awn9ayy6 123 4 ERp57 ERp57 NNS cord-001249-awn9ayy6 123 5 , , , cord-001249-awn9ayy6 123 6 another another DT cord-001249-awn9ayy6 123 7 soluble soluble JJ cord-001249-awn9ayy6 123 8 chaperone chaperone NN cord-001249-awn9ayy6 123 9 of of IN cord-001249-awn9ayy6 123 10 the the DT cord-001249-awn9ayy6 123 11 PDI PDI NNP cord-001249-awn9ayy6 123 12 - - HYPH cord-001249-awn9ayy6 123 13 like like JJ cord-001249-awn9ayy6 123 14 family family NN cord-001249-awn9ayy6 123 15 , , , cord-001249-awn9ayy6 123 16 was be VBD cord-001249-awn9ayy6 123 17 also also RB cord-001249-awn9ayy6 123 18 studied study VBN cord-001249-awn9ayy6 123 19 in in IN cord-001249-awn9ayy6 123 20 NSDVinfected nsdvinfected JJ cord-001249-awn9ayy6 123 21 cells cell NNS cord-001249-awn9ayy6 123 22 ; ; : cord-001249-awn9ayy6 123 23 again again RB cord-001249-awn9ayy6 123 24 many many JJ cord-001249-awn9ayy6 123 25 infected infected JJ cord-001249-awn9ayy6 123 26 cells cell NNS cord-001249-awn9ayy6 123 27 showed show VBD cord-001249-awn9ayy6 123 28 a a DT cord-001249-awn9ayy6 123 29 similar similar JJ cord-001249-awn9ayy6 123 30 redistribution redistribution NN cord-001249-awn9ayy6 123 31 or or CC cord-001249-awn9ayy6 123 32 complete complete JJ cord-001249-awn9ayy6 123 33 loss loss NN cord-001249-awn9ayy6 123 34 of of IN cord-001249-awn9ayy6 123 35 ERp57 ERp57 NNS cord-001249-awn9ayy6 123 36 from from IN cord-001249-awn9ayy6 123 37 the the DT cord-001249-awn9ayy6 123 38 ER er NN cord-001249-awn9ayy6 124 1 ( ( -LRB- cord-001249-awn9ayy6 124 2 Figure figure NN cord-001249-awn9ayy6 124 3 2 2 CD cord-001249-awn9ayy6 124 4 g g NN cord-001249-awn9ayy6 124 5 - - : cord-001249-awn9ayy6 124 6 i i NNP cord-001249-awn9ayy6 124 7 ) ) -RRB- cord-001249-awn9ayy6 124 8 , , , cord-001249-awn9ayy6 124 9 indicating indicate VBG cord-001249-awn9ayy6 124 10 that that IN cord-001249-awn9ayy6 124 11 other other JJ cord-001249-awn9ayy6 124 12 ER ER NNP cord-001249-awn9ayy6 124 13 - - HYPH cord-001249-awn9ayy6 124 14 lumenal lumenal JJ cord-001249-awn9ayy6 124 15 chaperones chaperone NNS cord-001249-awn9ayy6 124 16 / / SYM cord-001249-awn9ayy6 124 17 proteins protein NNS cord-001249-awn9ayy6 124 18 might may MD cord-001249-awn9ayy6 124 19 also also RB cord-001249-awn9ayy6 124 20 be be VB cord-001249-awn9ayy6 124 21 affected affect VBN cord-001249-awn9ayy6 124 22 . . . cord-001249-awn9ayy6 125 1 The the DT cord-001249-awn9ayy6 125 2 fraction fraction NN cord-001249-awn9ayy6 125 3 of of IN cord-001249-awn9ayy6 125 4 infected infected JJ cord-001249-awn9ayy6 125 5 cells cell NNS cord-001249-awn9ayy6 125 6 in in IN cord-001249-awn9ayy6 125 7 which which WDT cord-001249-awn9ayy6 125 8 PDI PDI NNP cord-001249-awn9ayy6 125 9 distribution distribution NN cord-001249-awn9ayy6 125 10 was be VBD cord-001249-awn9ayy6 125 11 affected affect VBN cord-001249-awn9ayy6 125 12 was be VBD cord-001249-awn9ayy6 125 13 quantified quantify VBN cord-001249-awn9ayy6 125 14 visually visually RB cord-001249-awn9ayy6 125 15 . . . cord-001249-awn9ayy6 126 1 The the DT cord-001249-awn9ayy6 126 2 amount amount NN cord-001249-awn9ayy6 126 3 of of IN cord-001249-awn9ayy6 126 4 PDI PDI NNP cord-001249-awn9ayy6 126 5 in in IN cord-001249-awn9ayy6 126 6 the the DT cord-001249-awn9ayy6 126 7 ER ER NNP cord-001249-awn9ayy6 126 8 of of IN cord-001249-awn9ayy6 126 9 150 150 CD cord-001249-awn9ayy6 126 10 infected infect VBN cord-001249-awn9ayy6 126 11 and and CC cord-001249-awn9ayy6 126 12 uninfected uninfected JJ cord-001249-awn9ayy6 126 13 cells cell NNS cord-001249-awn9ayy6 126 14 was be VBD cord-001249-awn9ayy6 126 15 investigated investigate VBN cord-001249-awn9ayy6 126 16 and and CC cord-001249-awn9ayy6 126 17 approximately approximately RB cord-001249-awn9ayy6 126 18 33 33 CD cord-001249-awn9ayy6 126 19 % % NN cord-001249-awn9ayy6 126 20 of of IN cord-001249-awn9ayy6 126 21 infected infected JJ cord-001249-awn9ayy6 126 22 cells cell NNS cord-001249-awn9ayy6 126 23 ( ( -LRB- cord-001249-awn9ayy6 126 24 53/150 53/150 CD cord-001249-awn9ayy6 126 25 ) ) -RRB- cord-001249-awn9ayy6 126 26 were be VBD cord-001249-awn9ayy6 126 27 found find VBN cord-001249-awn9ayy6 126 28 to to TO cord-001249-awn9ayy6 126 29 show show VB cord-001249-awn9ayy6 126 30 either either CC cord-001249-awn9ayy6 126 31 a a DT cord-001249-awn9ayy6 126 32 drastically drastically RB cord-001249-awn9ayy6 126 33 reduced reduce VBN cord-001249-awn9ayy6 126 34 level level NN cord-001249-awn9ayy6 126 35 or or CC cord-001249-awn9ayy6 126 36 total total JJ cord-001249-awn9ayy6 126 37 loss loss NN cord-001249-awn9ayy6 126 38 of of IN cord-001249-awn9ayy6 126 39 intracellular intracellular JJ cord-001249-awn9ayy6 126 40 PDI PDI NNP cord-001249-awn9ayy6 126 41 , , , cord-001249-awn9ayy6 126 42 while while IN cord-001249-awn9ayy6 126 43 only only RB cord-001249-awn9ayy6 126 44 1 1 CD cord-001249-awn9ayy6 126 45 uninfected uninfected JJ cord-001249-awn9ayy6 126 46 cell cell NN cord-001249-awn9ayy6 126 47 was be VBD cord-001249-awn9ayy6 126 48 seen see VBN cord-001249-awn9ayy6 126 49 with with IN cord-001249-awn9ayy6 126 50 low/ low/ NNP cord-001249-awn9ayy6 126 51 absent absent JJ cord-001249-awn9ayy6 126 52 PDI PDI NNP cord-001249-awn9ayy6 126 53 in in IN cord-001249-awn9ayy6 126 54 the the DT cord-001249-awn9ayy6 126 55 ER er NN cord-001249-awn9ayy6 127 1 ( ( -LRB- cord-001249-awn9ayy6 127 2 x x LS cord-001249-awn9ayy6 127 3 2 2 CD cord-001249-awn9ayy6 128 1 = = NFP cord-001249-awn9ayy6 128 2 58.7 58.7 CD cord-001249-awn9ayy6 128 3 , , , cord-001249-awn9ayy6 128 4 p,0.00001 p,0.00001 CD cord-001249-awn9ayy6 128 5 ) ) -RRB- cord-001249-awn9ayy6 128 6 . . . cord-001249-awn9ayy6 129 1 In in IN cord-001249-awn9ayy6 129 2 order order NN cord-001249-awn9ayy6 129 3 to to TO cord-001249-awn9ayy6 129 4 establish establish VB cord-001249-awn9ayy6 129 5 whether whether IN cord-001249-awn9ayy6 129 6 NSDV NSDV NNP cord-001249-awn9ayy6 129 7 replication replication NN cord-001249-awn9ayy6 129 8 affects affect VBZ cord-001249-awn9ayy6 129 9 the the DT cord-001249-awn9ayy6 129 10 structure structure NN cord-001249-awn9ayy6 129 11 of of IN cord-001249-awn9ayy6 129 12 the the DT cord-001249-awn9ayy6 129 13 entire entire JJ cord-001249-awn9ayy6 129 14 ER er NN cord-001249-awn9ayy6 129 15 or or CC cord-001249-awn9ayy6 129 16 just just RB cord-001249-awn9ayy6 129 17 the the DT cord-001249-awn9ayy6 129 18 distribution distribution NN cord-001249-awn9ayy6 129 19 of of IN cord-001249-awn9ayy6 129 20 specific specific JJ cord-001249-awn9ayy6 129 21 proteins protein NNS cord-001249-awn9ayy6 129 22 , , , cord-001249-awn9ayy6 129 23 we -PRON- PRP cord-001249-awn9ayy6 129 24 looked look VBD cord-001249-awn9ayy6 129 25 at at IN cord-001249-awn9ayy6 129 26 the the DT cord-001249-awn9ayy6 129 27 distribution distribution NN cord-001249-awn9ayy6 129 28 of of IN cord-001249-awn9ayy6 129 29 calnexin calnexin NNP cord-001249-awn9ayy6 129 30 , , , cord-001249-awn9ayy6 129 31 an an DT cord-001249-awn9ayy6 129 32 ERmembrane ERmembrane NNP cord-001249-awn9ayy6 129 33 chaperone chaperone NN cord-001249-awn9ayy6 129 34 , , , cord-001249-awn9ayy6 129 35 in in IN cord-001249-awn9ayy6 129 36 infected infected JJ cord-001249-awn9ayy6 129 37 and and CC cord-001249-awn9ayy6 129 38 uninfected uninfected JJ cord-001249-awn9ayy6 129 39 cells cell NNS cord-001249-awn9ayy6 129 40 . . . cord-001249-awn9ayy6 130 1 No no DT cord-001249-awn9ayy6 130 2 change change NN cord-001249-awn9ayy6 130 3 to to IN cord-001249-awn9ayy6 130 4 the the DT cord-001249-awn9ayy6 130 5 cellular cellular JJ cord-001249-awn9ayy6 130 6 distribution distribution NN cord-001249-awn9ayy6 130 7 of of IN cord-001249-awn9ayy6 130 8 calnexin calnexin NNP cord-001249-awn9ayy6 130 9 was be VBD cord-001249-awn9ayy6 130 10 observed observe VBN cord-001249-awn9ayy6 130 11 ( ( -LRB- cord-001249-awn9ayy6 130 12 Figure figure NN cord-001249-awn9ayy6 130 13 2 2 CD cord-001249-awn9ayy6 130 14 j j NNP cord-001249-awn9ayy6 130 15 - - HYPH cord-001249-awn9ayy6 130 16 l l NNP cord-001249-awn9ayy6 130 17 ) ) -RRB- cord-001249-awn9ayy6 130 18 , , , cord-001249-awn9ayy6 130 19 indicating indicate VBG cord-001249-awn9ayy6 130 20 that that IN cord-001249-awn9ayy6 130 21 , , , cord-001249-awn9ayy6 130 22 despite despite IN cord-001249-awn9ayy6 130 23 the the DT cord-001249-awn9ayy6 130 24 disappearance disappearance NN cord-001249-awn9ayy6 130 25 of of IN cord-001249-awn9ayy6 130 26 PDI PDI NNP cord-001249-awn9ayy6 130 27 and and CC cord-001249-awn9ayy6 130 28 ERp57 ERp57 NNS cord-001249-awn9ayy6 130 29 , , , cord-001249-awn9ayy6 130 30 the the DT cord-001249-awn9ayy6 130 31 overall overall JJ cord-001249-awn9ayy6 130 32 structure structure NN cord-001249-awn9ayy6 130 33 of of IN cord-001249-awn9ayy6 130 34 the the DT cord-001249-awn9ayy6 130 35 ER ER NNP cord-001249-awn9ayy6 130 36 remains remain VBZ cord-001249-awn9ayy6 130 37 unchanged unchanged JJ cord-001249-awn9ayy6 130 38 in in IN cord-001249-awn9ayy6 130 39 NSDV NSDV NNP cord-001249-awn9ayy6 130 40 - - HYPH cord-001249-awn9ayy6 130 41 infected infect VBN cord-001249-awn9ayy6 130 42 cells cell NNS cord-001249-awn9ayy6 130 43 . . . cord-001249-awn9ayy6 131 1 To to TO cord-001249-awn9ayy6 131 2 examine examine VB cord-001249-awn9ayy6 131 3 whether whether IN cord-001249-awn9ayy6 131 4 PDI PDI NNP cord-001249-awn9ayy6 131 5 and and CC cord-001249-awn9ayy6 131 6 ERp57 ERp57 NNS cord-001249-awn9ayy6 131 7 are be VBP cord-001249-awn9ayy6 131 8 selectively selectively RB cord-001249-awn9ayy6 131 9 degraded degrade VBN cord-001249-awn9ayy6 131 10 during during IN cord-001249-awn9ayy6 131 11 infection infection NN cord-001249-awn9ayy6 131 12 , , , cord-001249-awn9ayy6 131 13 Vero Vero NNP cord-001249-awn9ayy6 131 14 cells cell NNS cord-001249-awn9ayy6 131 15 were be VBD cord-001249-awn9ayy6 131 16 infected infect VBN cord-001249-awn9ayy6 131 17 with with IN cord-001249-awn9ayy6 131 18 NSDVi NSDVi NNS cord-001249-awn9ayy6 131 19 at at IN cord-001249-awn9ayy6 131 20 a a DT cord-001249-awn9ayy6 131 21 high high JJ cord-001249-awn9ayy6 131 22 MOI MOI NNP cord-001249-awn9ayy6 131 23 such such JJ cord-001249-awn9ayy6 131 24 that that IN cord-001249-awn9ayy6 131 25 all all DT cord-001249-awn9ayy6 131 26 cells cell NNS cord-001249-awn9ayy6 131 27 were be VBD cord-001249-awn9ayy6 131 28 infected infect VBN cord-001249-awn9ayy6 131 29 , , , cord-001249-awn9ayy6 131 30 and and CC cord-001249-awn9ayy6 131 31 total total JJ cord-001249-awn9ayy6 131 32 cellular cellular JJ cord-001249-awn9ayy6 131 33 levels level NNS cord-001249-awn9ayy6 131 34 of of IN cord-001249-awn9ayy6 131 35 PDI PDI NNP cord-001249-awn9ayy6 131 36 and and CC cord-001249-awn9ayy6 131 37 ERp57 ERp57 NNS cord-001249-awn9ayy6 131 38 in in IN cord-001249-awn9ayy6 131 39 these these DT cord-001249-awn9ayy6 131 40 cells cell NNS cord-001249-awn9ayy6 131 41 were be VBD cord-001249-awn9ayy6 131 42 analysed analyse VBN cord-001249-awn9ayy6 131 43 by by IN cord-001249-awn9ayy6 131 44 Western western JJ cord-001249-awn9ayy6 131 45 blotting blotting NN cord-001249-awn9ayy6 131 46 . . . cord-001249-awn9ayy6 132 1 For for IN cord-001249-awn9ayy6 132 2 comparison comparison NN cord-001249-awn9ayy6 132 3 , , , cord-001249-awn9ayy6 132 4 we -PRON- PRP cord-001249-awn9ayy6 132 5 also also RB cord-001249-awn9ayy6 132 6 determined determine VBD cord-001249-awn9ayy6 132 7 the the DT cord-001249-awn9ayy6 132 8 relative relative JJ cord-001249-awn9ayy6 132 9 levels level NNS cord-001249-awn9ayy6 132 10 of of IN cord-001249-awn9ayy6 132 11 calnexin calnexin NNP cord-001249-awn9ayy6 132 12 , , , cord-001249-awn9ayy6 132 13 GM130 GM130 NNP cord-001249-awn9ayy6 132 14 and and CC cord-001249-awn9ayy6 132 15 p102 p102 NNP cord-001249-awn9ayy6 132 16 ( ( -LRB- cord-001249-awn9ayy6 132 17 proteins protein NNS cord-001249-awn9ayy6 132 18 which which WDT cord-001249-awn9ayy6 132 19 showed show VBD cord-001249-awn9ayy6 132 20 no no DT cord-001249-awn9ayy6 132 21 effect effect NN cord-001249-awn9ayy6 132 22 of of IN cord-001249-awn9ayy6 132 23 infection infection NN cord-001249-awn9ayy6 132 24 on on IN cord-001249-awn9ayy6 132 25 their -PRON- PRP$ cord-001249-awn9ayy6 132 26 distribution distribution NN cord-001249-awn9ayy6 132 27 in in IN cord-001249-awn9ayy6 132 28 individual individual JJ cord-001249-awn9ayy6 132 29 cells cell NNS cord-001249-awn9ayy6 132 30 by by IN cord-001249-awn9ayy6 132 31 immunofluorescence immunofluorescence NN cord-001249-awn9ayy6 132 32 ) ) -RRB- cord-001249-awn9ayy6 132 33 ; ; : cord-001249-awn9ayy6 132 34 proliferating proliferate VBG cord-001249-awn9ayy6 132 35 cell cell NN cord-001249-awn9ayy6 132 36 nuclear nuclear JJ cord-001249-awn9ayy6 132 37 antigen antigen NN cord-001249-awn9ayy6 132 38 ( ( -LRB- cord-001249-awn9ayy6 132 39 PCNA PCNA NNP cord-001249-awn9ayy6 132 40 ) ) -RRB- cord-001249-awn9ayy6 132 41 used use VBD cord-001249-awn9ayy6 132 42 as as IN cord-001249-awn9ayy6 132 43 a a DT cord-001249-awn9ayy6 132 44 loading loading NN cord-001249-awn9ayy6 132 45 control control NN cord-001249-awn9ayy6 132 46 . . . cord-001249-awn9ayy6 133 1 Surprisingly surprisingly RB cord-001249-awn9ayy6 133 2 , , , cord-001249-awn9ayy6 133 3 given give VBN cord-001249-awn9ayy6 133 4 the the DT cord-001249-awn9ayy6 133 5 confocal confocal JJ cord-001249-awn9ayy6 133 6 results result NNS cord-001249-awn9ayy6 133 7 , , , cord-001249-awn9ayy6 133 8 NSDVi NSDVi NNS cord-001249-awn9ayy6 133 9 infection infection NN cord-001249-awn9ayy6 133 10 appeared appear VBD cord-001249-awn9ayy6 133 11 to to TO cord-001249-awn9ayy6 133 12 have have VB cord-001249-awn9ayy6 133 13 no no DT cord-001249-awn9ayy6 133 14 effect effect NN cord-001249-awn9ayy6 133 15 on on IN cord-001249-awn9ayy6 133 16 the the DT cord-001249-awn9ayy6 133 17 total total JJ cord-001249-awn9ayy6 133 18 PDI PDI NNP cord-001249-awn9ayy6 133 19 and and CC cord-001249-awn9ayy6 133 20 ERp57 erp57 JJ cord-001249-awn9ayy6 133 21 protein protein NN cord-001249-awn9ayy6 133 22 levels level NNS cord-001249-awn9ayy6 133 23 ( ( -LRB- cord-001249-awn9ayy6 133 24 Figure figure NN cord-001249-awn9ayy6 133 25 3 3 CD cord-001249-awn9ayy6 133 26 ) ) -RRB- cord-001249-awn9ayy6 133 27 . . . cord-001249-awn9ayy6 134 1 In in IN cord-001249-awn9ayy6 134 2 the the DT cord-001249-awn9ayy6 134 3 case case NN cord-001249-awn9ayy6 134 4 of of IN cord-001249-awn9ayy6 134 5 very very RB cord-001249-awn9ayy6 134 6 abundant abundant JJ cord-001249-awn9ayy6 134 7 proteins protein NNS cord-001249-awn9ayy6 134 8 such such JJ cord-001249-awn9ayy6 134 9 as as IN cord-001249-awn9ayy6 134 10 PDI PDI NNP cord-001249-awn9ayy6 134 11 , , , cord-001249-awn9ayy6 134 12 which which WDT cord-001249-awn9ayy6 134 13 represents represent VBZ cord-001249-awn9ayy6 134 14 around around RB cord-001249-awn9ayy6 134 15 1 1 CD cord-001249-awn9ayy6 134 16 % % NN cord-001249-awn9ayy6 134 17 of of IN cord-001249-awn9ayy6 134 18 total total JJ cord-001249-awn9ayy6 134 19 cellular cellular JJ cord-001249-awn9ayy6 134 20 proteins protein NNS cord-001249-awn9ayy6 134 21 ( ( -LRB- cord-001249-awn9ayy6 134 22 reviewed review VBN cord-001249-awn9ayy6 134 23 in in IN cord-001249-awn9ayy6 134 24 [ [ -LRB- cord-001249-awn9ayy6 134 25 69 69 CD cord-001249-awn9ayy6 134 26 ] ] -RRB- cord-001249-awn9ayy6 134 27 ) ) -RRB- cord-001249-awn9ayy6 134 28 , , , cord-001249-awn9ayy6 134 29 a a DT cord-001249-awn9ayy6 134 30 small small JJ cord-001249-awn9ayy6 134 31 difference difference NN cord-001249-awn9ayy6 134 32 in in IN cord-001249-awn9ayy6 134 33 protein protein NN cord-001249-awn9ayy6 134 34 level level NN cord-001249-awn9ayy6 134 35 might may MD cord-001249-awn9ayy6 134 36 not not RB cord-001249-awn9ayy6 134 37 be be VB cord-001249-awn9ayy6 134 38 detected detect VBN cord-001249-awn9ayy6 134 39 by by IN cord-001249-awn9ayy6 134 40 Western western JJ cord-001249-awn9ayy6 134 41 blotting blotting NN cord-001249-awn9ayy6 134 42 ; ; : cord-001249-awn9ayy6 134 43 however however RB cord-001249-awn9ayy6 134 44 , , , cord-001249-awn9ayy6 134 45 during during IN cord-001249-awn9ayy6 134 46 NSDV NSDV NNP cord-001249-awn9ayy6 134 47 infection infection NN cord-001249-awn9ayy6 134 48 , , , cord-001249-awn9ayy6 134 49 33 33 CD cord-001249-awn9ayy6 134 50 % % NN cord-001249-awn9ayy6 134 51 of of IN cord-001249-awn9ayy6 134 52 the the DT cord-001249-awn9ayy6 134 53 infected infected JJ cord-001249-awn9ayy6 134 54 cells cell NNS cord-001249-awn9ayy6 134 55 showed show VBD cord-001249-awn9ayy6 134 56 major major JJ cord-001249-awn9ayy6 134 57 or or CC cord-001249-awn9ayy6 134 58 total total JJ cord-001249-awn9ayy6 134 59 loss loss NN cord-001249-awn9ayy6 134 60 of of IN cord-001249-awn9ayy6 134 61 PDI PDI NNP cord-001249-awn9ayy6 134 62 ( ( -LRB- cord-001249-awn9ayy6 134 63 as as IN cord-001249-awn9ayy6 134 64 estimated estimate VBN cord-001249-awn9ayy6 134 65 using use VBG cord-001249-awn9ayy6 134 66 immunofluorescence immunofluorescence NN cord-001249-awn9ayy6 134 67 ) ) -RRB- cord-001249-awn9ayy6 134 68 , , , cord-001249-awn9ayy6 134 69 therefore therefore RB cord-001249-awn9ayy6 134 70 some some DT cord-001249-awn9ayy6 134 71 change change NN cord-001249-awn9ayy6 134 72 in in IN cord-001249-awn9ayy6 134 73 the the DT cord-001249-awn9ayy6 134 74 total total JJ cord-001249-awn9ayy6 134 75 PDI PDI NNP cord-001249-awn9ayy6 134 76 level level NN cord-001249-awn9ayy6 134 77 would would MD cord-001249-awn9ayy6 134 78 be be VB cord-001249-awn9ayy6 134 79 expected expect VBN cord-001249-awn9ayy6 134 80 to to TO cord-001249-awn9ayy6 134 81 be be VB cord-001249-awn9ayy6 134 82 seen see VBN cord-001249-awn9ayy6 134 83 if if IN cord-001249-awn9ayy6 134 84 this this DT cord-001249-awn9ayy6 134 85 protein protein NN cord-001249-awn9ayy6 134 86 was be VBD cord-001249-awn9ayy6 134 87 degraded degrade VBN cord-001249-awn9ayy6 134 88 in in IN cord-001249-awn9ayy6 134 89 the the DT cord-001249-awn9ayy6 134 90 infected infected JJ cord-001249-awn9ayy6 134 91 cells cell NNS cord-001249-awn9ayy6 134 92 . . . cord-001249-awn9ayy6 135 1 These these DT cord-001249-awn9ayy6 135 2 data datum NNS cord-001249-awn9ayy6 135 3 indicate indicate VBP cord-001249-awn9ayy6 135 4 that that IN cord-001249-awn9ayy6 135 5 , , , cord-001249-awn9ayy6 135 6 although although IN cord-001249-awn9ayy6 135 7 PDI PDI NNP cord-001249-awn9ayy6 135 8 and and CC cord-001249-awn9ayy6 135 9 ERp57 ERp57 NNS cord-001249-awn9ayy6 135 10 disappear disappear VBP cord-001249-awn9ayy6 135 11 from from IN cord-001249-awn9ayy6 135 12 the the DT cord-001249-awn9ayy6 135 13 ER ER NNP cord-001249-awn9ayy6 135 14 of of IN cord-001249-awn9ayy6 135 15 infected infected JJ cord-001249-awn9ayy6 135 16 cells cell NNS cord-001249-awn9ayy6 135 17 , , , cord-001249-awn9ayy6 135 18 this this DT cord-001249-awn9ayy6 135 19 is be VBZ cord-001249-awn9ayy6 135 20 not not RB cord-001249-awn9ayy6 135 21 a a DT cord-001249-awn9ayy6 135 22 reflection reflection NN cord-001249-awn9ayy6 135 23 of of IN cord-001249-awn9ayy6 135 24 overall overall JJ cord-001249-awn9ayy6 135 25 loss loss NN cord-001249-awn9ayy6 135 26 from from IN cord-001249-awn9ayy6 135 27 the the DT cord-001249-awn9ayy6 135 28 cell cell NN cord-001249-awn9ayy6 135 29 such such JJ cord-001249-awn9ayy6 135 30 as as IN cord-001249-awn9ayy6 135 31 through through IN cord-001249-awn9ayy6 135 32 degradation degradation NN cord-001249-awn9ayy6 135 33 . . . cord-001249-awn9ayy6 136 1 Export export NN cord-001249-awn9ayy6 136 2 of of IN cord-001249-awn9ayy6 136 3 ER ER NNP cord-001249-awn9ayy6 136 4 Proteins Proteins NNP cord-001249-awn9ayy6 136 5 in in IN cord-001249-awn9ayy6 136 6 Nairovirus Nairovirus NNP cord-001249-awn9ayy6 136 7 - - HYPH cord-001249-awn9ayy6 136 8 Infected Infected NNP cord-001249-awn9ayy6 136 9 Cells cell NNS cord-001249-awn9ayy6 137 1 PLOS PLOS NNP cord-001249-awn9ayy6 138 1 ONE one CD cord-001249-awn9ayy6 138 2 | | HYPH cord-001249-awn9ayy6 138 3 www.plosone.org www.plosone.org NN cord-001249-awn9ayy6 138 4 PDI PDI NNP cord-001249-awn9ayy6 138 5 and and CC cord-001249-awn9ayy6 138 6 ERp57 ERp57 NNS cord-001249-awn9ayy6 138 7 appear appear VBP cord-001249-awn9ayy6 138 8 at at IN cord-001249-awn9ayy6 138 9 the the DT cord-001249-awn9ayy6 138 10 surface surface NN cord-001249-awn9ayy6 138 11 of of IN cord-001249-awn9ayy6 138 12 many many JJ cord-001249-awn9ayy6 138 13 NSDVinfected nsdvinfecte VBN cord-001249-awn9ayy6 138 14 cells cell NNS cord-001249-awn9ayy6 138 15 and and CC cord-001249-awn9ayy6 138 16 are be VBP cord-001249-awn9ayy6 138 17 secreted secrete VBN cord-001249-awn9ayy6 138 18 from from IN cord-001249-awn9ayy6 138 19 these these DT cord-001249-awn9ayy6 138 20 cells cell NNS cord-001249-awn9ayy6 139 1 The the DT cord-001249-awn9ayy6 139 2 absence absence NN cord-001249-awn9ayy6 139 3 of of IN cord-001249-awn9ayy6 139 4 PDI PDI NNP cord-001249-awn9ayy6 139 5 and and CC cord-001249-awn9ayy6 139 6 ERp57 erp57 JJ cord-001249-awn9ayy6 139 7 degradation degradation NN cord-001249-awn9ayy6 139 8 suggested suggest VBD cord-001249-awn9ayy6 139 9 that that IN cord-001249-awn9ayy6 139 10 , , , cord-001249-awn9ayy6 139 11 although although IN cord-001249-awn9ayy6 139 12 these these DT cord-001249-awn9ayy6 139 13 proteins protein NNS cord-001249-awn9ayy6 139 14 disappear disappear VBP cord-001249-awn9ayy6 139 15 from from IN cord-001249-awn9ayy6 139 16 the the DT cord-001249-awn9ayy6 139 17 ER ER NNP cord-001249-awn9ayy6 139 18 , , , cord-001249-awn9ayy6 139 19 they -PRON- PRP cord-001249-awn9ayy6 139 20 somehow somehow RB cord-001249-awn9ayy6 139 21 remain remain VBP cord-001249-awn9ayy6 139 22 associated associated JJ cord-001249-awn9ayy6 139 23 with with IN cord-001249-awn9ayy6 139 24 the the DT cord-001249-awn9ayy6 139 25 NSDV NSDV NNP cord-001249-awn9ayy6 139 26 - - HYPH cord-001249-awn9ayy6 139 27 infected infect VBN cord-001249-awn9ayy6 139 28 cells cell NNS cord-001249-awn9ayy6 139 29 . . . cord-001249-awn9ayy6 140 1 One one CD cord-001249-awn9ayy6 140 2 possibility possibility NN cord-001249-awn9ayy6 140 3 was be VBD cord-001249-awn9ayy6 140 4 that that IN cord-001249-awn9ayy6 140 5 these these DT cord-001249-awn9ayy6 140 6 proteins protein NNS cord-001249-awn9ayy6 140 7 are be VBP cord-001249-awn9ayy6 140 8 moving move VBG cord-001249-awn9ayy6 140 9 to to IN cord-001249-awn9ayy6 140 10 the the DT cord-001249-awn9ayy6 140 11 cell cell NN cord-001249-awn9ayy6 140 12 surface surface NN cord-001249-awn9ayy6 140 13 in in IN cord-001249-awn9ayy6 140 14 infected infected JJ cord-001249-awn9ayy6 140 15 cells cell NNS cord-001249-awn9ayy6 140 16 , , , cord-001249-awn9ayy6 140 17 but but CC cord-001249-awn9ayy6 140 18 are be VBP cord-001249-awn9ayy6 140 19 lost lose VBN cord-001249-awn9ayy6 140 20 during during IN cord-001249-awn9ayy6 140 21 fixation fixation NN cord-001249-awn9ayy6 140 22 and and CC cord-001249-awn9ayy6 140 23 washing washing NN cord-001249-awn9ayy6 140 24 , , , cord-001249-awn9ayy6 140 25 since since IN cord-001249-awn9ayy6 140 26 PDI PDI NNP cord-001249-awn9ayy6 140 27 is be VBZ cord-001249-awn9ayy6 140 28 known know VBN cord-001249-awn9ayy6 140 29 to to TO cord-001249-awn9ayy6 140 30 be be VB cord-001249-awn9ayy6 140 31 only only RB cord-001249-awn9ayy6 140 32 weakly weakly RB cord-001249-awn9ayy6 140 33 bound bind VBN cord-001249-awn9ayy6 140 34 to to IN cord-001249-awn9ayy6 140 35 the the DT cord-001249-awn9ayy6 140 36 cell cell NN cord-001249-awn9ayy6 140 37 surface surface NN cord-001249-awn9ayy6 140 38 via via IN cord-001249-awn9ayy6 140 39 non non JJ cord-001249-awn9ayy6 140 40 - - JJ cord-001249-awn9ayy6 140 41 covalent covalent JJ cord-001249-awn9ayy6 140 42 interactions interaction NNS cord-001249-awn9ayy6 140 43 with with IN cord-001249-awn9ayy6 140 44 other other JJ cord-001249-awn9ayy6 140 45 molecules molecule NNS cord-001249-awn9ayy6 140 46 present present JJ cord-001249-awn9ayy6 140 47 at at IN cord-001249-awn9ayy6 140 48 the the DT cord-001249-awn9ayy6 140 49 cell cell NN cord-001249-awn9ayy6 140 50 surface surface NN cord-001249-awn9ayy6 140 51 [ [ -LRB- cord-001249-awn9ayy6 140 52 70 70 CD cord-001249-awn9ayy6 140 53 ] ] -RRB- cord-001249-awn9ayy6 140 54 . . . cord-001249-awn9ayy6 141 1 This this DT cord-001249-awn9ayy6 141 2 possibility possibility NN cord-001249-awn9ayy6 141 3 was be VBD cord-001249-awn9ayy6 141 4 investigated investigate VBN cord-001249-awn9ayy6 141 5 using use VBG cord-001249-awn9ayy6 141 6 careful careful JJ cord-001249-awn9ayy6 141 7 surface surface NN cord-001249-awn9ayy6 141 8 labelling labelling NN cord-001249-awn9ayy6 141 9 of of IN cord-001249-awn9ayy6 141 10 lightly lightly RB cord-001249-awn9ayy6 141 11 fixed fix VBN cord-001249-awn9ayy6 141 12 cells cell NNS cord-001249-awn9ayy6 141 13 and and CC cord-001249-awn9ayy6 141 14 confocal confocal JJ cord-001249-awn9ayy6 141 15 microscopy microscopy NNS cord-001249-awn9ayy6 141 16 . . . cord-001249-awn9ayy6 142 1 To to TO cord-001249-awn9ayy6 142 2 detect detect VB cord-001249-awn9ayy6 142 3 PDI PDI NNP cord-001249-awn9ayy6 142 4 and and CC cord-001249-awn9ayy6 142 5 ERp57 ERp57 NNS cord-001249-awn9ayy6 142 6 at at IN cord-001249-awn9ayy6 142 7 the the DT cord-001249-awn9ayy6 142 8 surface surface NN cord-001249-awn9ayy6 142 9 , , , cord-001249-awn9ayy6 142 10 Vero Vero NNP cord-001249-awn9ayy6 142 11 cells cell NNS cord-001249-awn9ayy6 142 12 infected infect VBN cord-001249-awn9ayy6 142 13 with with IN cord-001249-awn9ayy6 142 14 NSDVi NSDVi NNS cord-001249-awn9ayy6 142 15 at at IN cord-001249-awn9ayy6 142 16 a a DT cord-001249-awn9ayy6 142 17 low low JJ cord-001249-awn9ayy6 142 18 MOI MOI NNP cord-001249-awn9ayy6 142 19 were be VBD cord-001249-awn9ayy6 142 20 fixed fix VBN cord-001249-awn9ayy6 142 21 and and CC cord-001249-awn9ayy6 142 22 then then RB cord-001249-awn9ayy6 142 23 incubated incubate VBN cord-001249-awn9ayy6 142 24 with with IN cord-001249-awn9ayy6 142 25 anti anti JJ cord-001249-awn9ayy6 142 26 - - JJ cord-001249-awn9ayy6 142 27 PDI pdi JJ cord-001249-awn9ayy6 142 28 or or CC cord-001249-awn9ayy6 142 29 anti anti JJ cord-001249-awn9ayy6 142 30 - - JJ cord-001249-awn9ayy6 142 31 ERp57 erp57 JJ cord-001249-awn9ayy6 142 32 antibody antibody NN cord-001249-awn9ayy6 142 33 without without IN cord-001249-awn9ayy6 142 34 permeabilization permeabilization NN cord-001249-awn9ayy6 142 35 . . . cord-001249-awn9ayy6 143 1 After after IN cord-001249-awn9ayy6 143 2 washing wash VBG cord-001249-awn9ayy6 143 3 away away RP cord-001249-awn9ayy6 143 4 unbound unbound JJ cord-001249-awn9ayy6 143 5 antibodies antibody NNS cord-001249-awn9ayy6 143 6 , , , cord-001249-awn9ayy6 143 7 cells cell NNS cord-001249-awn9ayy6 143 8 were be VBD cord-001249-awn9ayy6 143 9 again again RB cord-001249-awn9ayy6 143 10 fixed fix VBN cord-001249-awn9ayy6 143 11 with with IN cord-001249-awn9ayy6 143 12 3 3 CD cord-001249-awn9ayy6 143 13 % % NN cord-001249-awn9ayy6 143 14 PFA PFA NNP cord-001249-awn9ayy6 143 15 to to TO cord-001249-awn9ayy6 143 16 ensure ensure VB cord-001249-awn9ayy6 143 17 that that IN cord-001249-awn9ayy6 143 18 any any DT cord-001249-awn9ayy6 143 19 PDI PDI NNP cord-001249-awn9ayy6 143 20 / / SYM cord-001249-awn9ayy6 143 21 ERp57 ERp57 NNS cord-001249-awn9ayy6 143 22 and and CC cord-001249-awn9ayy6 143 23 bound bind VBN cord-001249-awn9ayy6 143 24 antibodies antibody NNS cord-001249-awn9ayy6 143 25 remained remain VBD cord-001249-awn9ayy6 143 26 attached attach VBN cord-001249-awn9ayy6 143 27 to to IN cord-001249-awn9ayy6 143 28 the the DT cord-001249-awn9ayy6 143 29 cell cell NN cord-001249-awn9ayy6 143 30 surface surface NN cord-001249-awn9ayy6 143 31 throughout throughout IN cord-001249-awn9ayy6 143 32 the the DT cord-001249-awn9ayy6 143 33 staining staining NN cord-001249-awn9ayy6 143 34 procedure procedure NN cord-001249-awn9ayy6 143 35 . . . cord-001249-awn9ayy6 144 1 To to TO cord-001249-awn9ayy6 144 2 detect detect VB cord-001249-awn9ayy6 144 3 NSDV nsdv NN cord-001249-awn9ayy6 144 4 proteins protein NNS cord-001249-awn9ayy6 144 5 , , , cord-001249-awn9ayy6 144 6 cells cell NNS cord-001249-awn9ayy6 144 7 were be VBD cord-001249-awn9ayy6 144 8 then then RB cord-001249-awn9ayy6 144 9 permeabilised permeabilise VBN cord-001249-awn9ayy6 144 10 with with IN cord-001249-awn9ayy6 144 11 ice ice NN cord-001249-awn9ayy6 144 12 - - HYPH cord-001249-awn9ayy6 144 13 cold cold JJ cord-001249-awn9ayy6 144 14 methanol methanol NN cord-001249-awn9ayy6 144 15 and and CC cord-001249-awn9ayy6 144 16 incubated incubate VBN cord-001249-awn9ayy6 144 17 with with IN cord-001249-awn9ayy6 144 18 rabbit rabbit NN cord-001249-awn9ayy6 144 19 anti anti JJ cord-001249-awn9ayy6 144 20 - - NNP cord-001249-awn9ayy6 144 21 NSDV NSDV NNP cord-001249-awn9ayy6 144 22 N N NNP cord-001249-awn9ayy6 144 23 protein protein NN cord-001249-awn9ayy6 144 24 serum serum NN cord-001249-awn9ayy6 144 25 or or CC cord-001249-awn9ayy6 144 26 with with IN cord-001249-awn9ayy6 144 27 mouse mouse NN cord-001249-awn9ayy6 144 28 anti anti NNS cord-001249-awn9ayy6 144 29 - - NNS cord-001249-awn9ayy6 144 30 NSDVi NSDVi NNS cord-001249-awn9ayy6 145 1 PreGn pregn PRP cord-001249-awn9ayy6 145 2 ( ( -LRB- cord-001249-awn9ayy6 145 3 a a DT cord-001249-awn9ayy6 145 4 monoclonal monoclonal NN cord-001249-awn9ayy6 145 5 which which WDT cord-001249-awn9ayy6 145 6 recognises recognise VBZ cord-001249-awn9ayy6 145 7 a a DT cord-001249-awn9ayy6 145 8 sequence sequence NN cord-001249-awn9ayy6 145 9 near near IN cord-001249-awn9ayy6 145 10 the the DT cord-001249-awn9ayy6 145 11 amino amino NN cord-001249-awn9ayy6 145 12 - - HYPH cord-001249-awn9ayy6 145 13 terminus terminus NN cord-001249-awn9ayy6 145 14 of of IN cord-001249-awn9ayy6 145 15 the the DT cord-001249-awn9ayy6 145 16 NSDVi NSDVi NNS cord-001249-awn9ayy6 145 17 PreGn PreGn NNP cord-001249-awn9ayy6 145 18 glycoprotein glycoprotein NN cord-001249-awn9ayy6 145 19 ) ) -RRB- cord-001249-awn9ayy6 145 20 . . . cord-001249-awn9ayy6 146 1 We -PRON- PRP cord-001249-awn9ayy6 146 2 observed observe VBD cord-001249-awn9ayy6 146 3 that that IN cord-001249-awn9ayy6 146 4 many many JJ cord-001249-awn9ayy6 146 5 NSDV nsdv NN cord-001249-awn9ayy6 146 6 - - HYPH cord-001249-awn9ayy6 146 7 infected infect VBN cord-001249-awn9ayy6 146 8 cells cell NNS cord-001249-awn9ayy6 146 9 showed show VBD cord-001249-awn9ayy6 146 10 significant significant JJ cord-001249-awn9ayy6 146 11 amounts amount NNS cord-001249-awn9ayy6 146 12 of of IN cord-001249-awn9ayy6 146 13 cell cell NN cord-001249-awn9ayy6 146 14 surface surface NN cord-001249-awn9ayy6 146 15 PDI PDI NNP cord-001249-awn9ayy6 146 16 and and CC cord-001249-awn9ayy6 146 17 ERp57 ERp57 NNS cord-001249-awn9ayy6 146 18 ( ( -LRB- cord-001249-awn9ayy6 146 19 Figure figure NN cord-001249-awn9ayy6 146 20 4 4 CD cord-001249-awn9ayy6 146 21 .A .A . cord-001249-awn9ayy6 146 22 ) ) -RRB- cord-001249-awn9ayy6 146 23 . . . cord-001249-awn9ayy6 147 1 Although although IN cord-001249-awn9ayy6 147 2 small small JJ cord-001249-awn9ayy6 147 3 amounts amount NNS cord-001249-awn9ayy6 147 4 of of IN cord-001249-awn9ayy6 147 5 PDI PDI NNP cord-001249-awn9ayy6 147 6 and and CC cord-001249-awn9ayy6 147 7 ERp57 ERp57 NNS cord-001249-awn9ayy6 147 8 could could MD cord-001249-awn9ayy6 147 9 be be VB cord-001249-awn9ayy6 147 10 observed observe VBN cord-001249-awn9ayy6 147 11 at at IN cord-001249-awn9ayy6 147 12 the the DT cord-001249-awn9ayy6 147 13 surface surface NN cord-001249-awn9ayy6 147 14 of of IN cord-001249-awn9ayy6 147 15 even even RB cord-001249-awn9ayy6 147 16 uninfected uninfected JJ cord-001249-awn9ayy6 147 17 cells cell NNS cord-001249-awn9ayy6 147 18 , , , cord-001249-awn9ayy6 147 19 NSDV NSDV NNP cord-001249-awn9ayy6 147 20 infection infection NN cord-001249-awn9ayy6 147 21 led lead VBD cord-001249-awn9ayy6 147 22 to to IN cord-001249-awn9ayy6 147 23 a a DT cord-001249-awn9ayy6 147 24 large large JJ cord-001249-awn9ayy6 147 25 increase increase NN cord-001249-awn9ayy6 147 26 in in IN cord-001249-awn9ayy6 147 27 the the DT cord-001249-awn9ayy6 147 28 amount amount NN cord-001249-awn9ayy6 147 29 of of IN cord-001249-awn9ayy6 147 30 these these DT cord-001249-awn9ayy6 147 31 proteins protein NNS cord-001249-awn9ayy6 147 32 at at IN cord-001249-awn9ayy6 147 33 the the DT cord-001249-awn9ayy6 147 34 surface surface NN cord-001249-awn9ayy6 147 35 . . . cord-001249-awn9ayy6 148 1 Approximately approximately RB cord-001249-awn9ayy6 148 2 1/3 1/3 CD cord-001249-awn9ayy6 148 3 rd rd NN cord-001249-awn9ayy6 148 4 of of IN cord-001249-awn9ayy6 148 5 infected infected JJ cord-001249-awn9ayy6 148 6 cells cell NNS cord-001249-awn9ayy6 148 7 ( ( -LRB- cord-001249-awn9ayy6 148 8 calculated calculate VBN cord-001249-awn9ayy6 148 9 as as IN cord-001249-awn9ayy6 148 10 a a DT cord-001249-awn9ayy6 148 11 fraction fraction NN cord-001249-awn9ayy6 148 12 ( ( -LRB- cord-001249-awn9ayy6 148 13 51/150 51/150 CD cord-001249-awn9ayy6 148 14 ) ) -RRB- cord-001249-awn9ayy6 148 15 of of IN cord-001249-awn9ayy6 148 16 viral viral JJ cord-001249-awn9ayy6 148 17 proteinpositive proteinpositive JJ cord-001249-awn9ayy6 148 18 cells cell NNS cord-001249-awn9ayy6 148 19 with with IN cord-001249-awn9ayy6 148 20 increased increase VBN cord-001249-awn9ayy6 148 21 surface surface NN cord-001249-awn9ayy6 148 22 PDI PDI NNP cord-001249-awn9ayy6 148 23 / / SYM cord-001249-awn9ayy6 148 24 ERp57 ERp57 NNS cord-001249-awn9ayy6 148 25 calculated calculate VBN cord-001249-awn9ayy6 148 26 over over IN cord-001249-awn9ayy6 148 27 several several JJ cord-001249-awn9ayy6 148 28 focal focal JJ cord-001249-awn9ayy6 148 29 views view NNS cord-001249-awn9ayy6 148 30 ) ) -RRB- cord-001249-awn9ayy6 148 31 showed show VBD cord-001249-awn9ayy6 148 32 this this DT cord-001249-awn9ayy6 148 33 high high JJ cord-001249-awn9ayy6 148 34 level level NN cord-001249-awn9ayy6 148 35 of of IN cord-001249-awn9ayy6 148 36 surface surface NN cord-001249-awn9ayy6 148 37 PDI PDI NNP cord-001249-awn9ayy6 148 38 / / SYM cord-001249-awn9ayy6 148 39 ERp57 ERp57 NNS cord-001249-awn9ayy6 148 40 , , , cord-001249-awn9ayy6 148 41 and and CC cord-001249-awn9ayy6 148 42 only only RB cord-001249-awn9ayy6 148 43 1/150 1/150 CD cord-001249-awn9ayy6 148 44 uninfected uninfected JJ cord-001249-awn9ayy6 148 45 cells cell NNS cord-001249-awn9ayy6 148 46 showed show VBD cord-001249-awn9ayy6 148 47 increased increase VBN cord-001249-awn9ayy6 148 48 surface surface NN cord-001249-awn9ayy6 148 49 PDI/ PDI/ NNP cord-001249-awn9ayy6 148 50 ERp57 ERp57 NNS cord-001249-awn9ayy6 148 51 ( ( -LRB- cord-001249-awn9ayy6 148 52 x x NN cord-001249-awn9ayy6 148 53 2 2 CD cord-001249-awn9ayy6 148 54 = = SYM cord-001249-awn9ayy6 148 55 55.8 55.8 CD cord-001249-awn9ayy6 148 56 , , , cord-001249-awn9ayy6 148 57 p,0.00001 p,0.00001 CD cord-001249-awn9ayy6 148 58 ) ) -RRB- cord-001249-awn9ayy6 148 59 . . . cord-001249-awn9ayy6 149 1 We -PRON- PRP cord-001249-awn9ayy6 149 2 did do VBD cord-001249-awn9ayy6 149 3 observe observe VB cord-001249-awn9ayy6 149 4 a a DT cord-001249-awn9ayy6 149 5 small small JJ cord-001249-awn9ayy6 149 6 fraction fraction NN cord-001249-awn9ayy6 149 7 of of IN cord-001249-awn9ayy6 149 8 cells cell NNS cord-001249-awn9ayy6 149 9 the the DT cord-001249-awn9ayy6 149 10 membranes membrane NNS cord-001249-awn9ayy6 149 11 of of IN cord-001249-awn9ayy6 149 12 which which WDT cord-001249-awn9ayy6 149 13 were be VBD cord-001249-awn9ayy6 149 14 permeabilised permeabilise VBN cord-001249-awn9ayy6 149 15 by by IN cord-001249-awn9ayy6 149 16 the the DT cord-001249-awn9ayy6 149 17 initial initial JJ cord-001249-awn9ayy6 149 18 fixation fixation NN cord-001249-awn9ayy6 149 19 , , , cord-001249-awn9ayy6 149 20 leading lead VBG cord-001249-awn9ayy6 149 21 to to IN cord-001249-awn9ayy6 149 22 labelling labelling NN cord-001249-awn9ayy6 149 23 of of IN cord-001249-awn9ayy6 149 24 internal internal JJ cord-001249-awn9ayy6 149 25 PDI PDI NNP cord-001249-awn9ayy6 149 26 or or CC cord-001249-awn9ayy6 149 27 ERp57 ERp57 NNS cord-001249-awn9ayy6 149 28 ; ; : cord-001249-awn9ayy6 149 29 however however RB cord-001249-awn9ayy6 149 30 , , , cord-001249-awn9ayy6 149 31 surface surface NN cord-001249-awn9ayy6 149 32 PDI PDI NNP cord-001249-awn9ayy6 149 33 / / SYM cord-001249-awn9ayy6 149 34 ERp57 ERp57 NNS cord-001249-awn9ayy6 149 35 staining staining NN cord-001249-awn9ayy6 149 36 showed show VBD cord-001249-awn9ayy6 149 37 a a DT cord-001249-awn9ayy6 149 38 completely completely RB cord-001249-awn9ayy6 149 39 different different JJ cord-001249-awn9ayy6 149 40 pattern pattern NN cord-001249-awn9ayy6 149 41 to to IN cord-001249-awn9ayy6 149 42 the the DT cord-001249-awn9ayy6 149 43 resultant resultant JJ cord-001249-awn9ayy6 149 44 intracellular intracellular JJ cord-001249-awn9ayy6 149 45 ( ( -LRB- cord-001249-awn9ayy6 149 46 ER er NN cord-001249-awn9ayy6 149 47 ) ) -RRB- cord-001249-awn9ayy6 149 48 staining staining NN cord-001249-awn9ayy6 149 49 . . . cord-001249-awn9ayy6 150 1 To to TO cord-001249-awn9ayy6 150 2 ensure ensure VB cord-001249-awn9ayy6 150 3 that that IN cord-001249-awn9ayy6 150 4 the the DT cord-001249-awn9ayy6 150 5 apparent apparent JJ cord-001249-awn9ayy6 150 6 surface surface NN cord-001249-awn9ayy6 150 7 PDI PDI NNP cord-001249-awn9ayy6 150 8 is be VBZ cord-001249-awn9ayy6 150 9 not not RB cord-001249-awn9ayy6 150 10 an an DT cord-001249-awn9ayy6 150 11 artefact artefact NN cord-001249-awn9ayy6 150 12 of of IN cord-001249-awn9ayy6 150 13 the the DT cord-001249-awn9ayy6 150 14 multi multi JJ cord-001249-awn9ayy6 150 15 - - JJ cord-001249-awn9ayy6 150 16 step step JJ cord-001249-awn9ayy6 150 17 labelling labelling NN cord-001249-awn9ayy6 150 18 procedure procedure NN cord-001249-awn9ayy6 150 19 and and CC cord-001249-awn9ayy6 150 20 that that IN cord-001249-awn9ayy6 150 21 anti anti JJ cord-001249-awn9ayy6 150 22 - - JJ cord-001249-awn9ayy6 150 23 PDI pdi JJ cord-001249-awn9ayy6 150 24 antibody antibody NN cord-001249-awn9ayy6 150 25 does do VBZ cord-001249-awn9ayy6 150 26 not not RB cord-001249-awn9ayy6 150 27 re re VB cord-001249-awn9ayy6 150 28 - - NN cord-001249-awn9ayy6 150 29 associate associate VB cord-001249-awn9ayy6 150 30 with with IN cord-001249-awn9ayy6 150 31 an an DT cord-001249-awn9ayy6 150 32 internal internal JJ cord-001249-awn9ayy6 150 33 epitope epitope NN cord-001249-awn9ayy6 150 34 after after IN cord-001249-awn9ayy6 150 35 opening opening NN cord-001249-awn9ayy6 150 36 of of IN cord-001249-awn9ayy6 150 37 cells cell NNS cord-001249-awn9ayy6 150 38 with with IN cord-001249-awn9ayy6 150 39 methanol methanol NN cord-001249-awn9ayy6 150 40 , , , cord-001249-awn9ayy6 150 41 we -PRON- PRP cord-001249-awn9ayy6 150 42 infected infect VBD cord-001249-awn9ayy6 150 43 cells cell NNS cord-001249-awn9ayy6 150 44 at at IN cord-001249-awn9ayy6 150 45 a a DT cord-001249-awn9ayy6 150 46 high high JJ cord-001249-awn9ayy6 150 47 MOI MOI NNP cord-001249-awn9ayy6 150 48 and and CC cord-001249-awn9ayy6 150 49 repeated repeat VBD cord-001249-awn9ayy6 150 50 the the DT cord-001249-awn9ayy6 150 51 surface surface NN cord-001249-awn9ayy6 150 52 labelling labelling NN cord-001249-awn9ayy6 150 53 of of IN cord-001249-awn9ayy6 150 54 non non JJ cord-001249-awn9ayy6 150 55 - - JJ cord-001249-awn9ayy6 150 56 permeabilised permeabilised JJ cord-001249-awn9ayy6 150 57 cells cell NNS cord-001249-awn9ayy6 150 58 using use VBG cord-001249-awn9ayy6 150 59 anti anti JJ cord-001249-awn9ayy6 150 60 - - JJ cord-001249-awn9ayy6 150 61 PDI pdi JJ cord-001249-awn9ayy6 150 62 antibody antibody NN cord-001249-awn9ayy6 150 63 as as IN cord-001249-awn9ayy6 150 64 previously previously RB cord-001249-awn9ayy6 150 65 , , , cord-001249-awn9ayy6 150 66 but but CC cord-001249-awn9ayy6 150 67 omitting omit VBG cord-001249-awn9ayy6 150 68 the the DT cord-001249-awn9ayy6 150 69 subsequent subsequent JJ cord-001249-awn9ayy6 150 70 permeabilization permeabilization NN cord-001249-awn9ayy6 150 71 step step NN cord-001249-awn9ayy6 150 72 and and CC cord-001249-awn9ayy6 150 73 staining stain VBG cord-001249-awn9ayy6 150 74 for for IN cord-001249-awn9ayy6 150 75 viral viral JJ cord-001249-awn9ayy6 150 76 protein protein NN cord-001249-awn9ayy6 150 77 . . . cord-001249-awn9ayy6 151 1 Enhanced enhanced JJ cord-001249-awn9ayy6 151 2 surface surface NN cord-001249-awn9ayy6 151 3 PDI PDI NNP cord-001249-awn9ayy6 151 4 was be VBD cord-001249-awn9ayy6 151 5 detected detect VBN cord-001249-awn9ayy6 151 6 in in IN cord-001249-awn9ayy6 151 7 batches batch NNS cord-001249-awn9ayy6 151 8 of of IN cord-001249-awn9ayy6 151 9 infected infected JJ cord-001249-awn9ayy6 151 10 but but CC cord-001249-awn9ayy6 151 11 not not RB cord-001249-awn9ayy6 151 12 in in IN cord-001249-awn9ayy6 151 13 uninfected uninfected JJ cord-001249-awn9ayy6 151 14 cells cell NNS cord-001249-awn9ayy6 151 15 ( ( -LRB- cord-001249-awn9ayy6 151 16 Figure figure NN cord-001249-awn9ayy6 151 17 4 4 CD cord-001249-awn9ayy6 151 18 .B .B NFP cord-001249-awn9ayy6 151 19 ) ) -RRB- cord-001249-awn9ayy6 151 20 , , , cord-001249-awn9ayy6 151 21 confirming confirm VBG cord-001249-awn9ayy6 151 22 that that IN cord-001249-awn9ayy6 151 23 surface surface NN cord-001249-awn9ayy6 151 24 PDI PDI NNP cord-001249-awn9ayy6 151 25 detected detect VBD cord-001249-awn9ayy6 151 26 in in IN cord-001249-awn9ayy6 151 27 NSDVinfected nsdvinfected JJ cord-001249-awn9ayy6 151 28 cells cell NNS cord-001249-awn9ayy6 151 29 is be VBZ cord-001249-awn9ayy6 151 30 not not RB cord-001249-awn9ayy6 151 31 an an DT cord-001249-awn9ayy6 151 32 artefact artefact NN cord-001249-awn9ayy6 151 33 of of IN cord-001249-awn9ayy6 151 34 the the DT cord-001249-awn9ayy6 151 35 staining staining NN cord-001249-awn9ayy6 151 36 procedure procedure NN cord-001249-awn9ayy6 151 37 . . . cord-001249-awn9ayy6 152 1 The the DT cord-001249-awn9ayy6 152 2 disappearance disappearance NN cord-001249-awn9ayy6 152 3 of of IN cord-001249-awn9ayy6 152 4 PDI PDI NNP cord-001249-awn9ayy6 152 5 / / SYM cord-001249-awn9ayy6 152 6 ERp57 ERp57 NNS cord-001249-awn9ayy6 152 7 from from IN cord-001249-awn9ayy6 152 8 the the DT cord-001249-awn9ayy6 152 9 ER ER NNP cord-001249-awn9ayy6 152 10 and and CC cord-001249-awn9ayy6 152 11 their -PRON- PRP$ cord-001249-awn9ayy6 152 12 appearance appearance NN cord-001249-awn9ayy6 152 13 at at IN cord-001249-awn9ayy6 152 14 the the DT cord-001249-awn9ayy6 152 15 cell cell NN cord-001249-awn9ayy6 152 16 surface surface NN cord-001249-awn9ayy6 152 17 in in IN cord-001249-awn9ayy6 152 18 infected infected JJ cord-001249-awn9ayy6 152 19 cells cell NNS cord-001249-awn9ayy6 152 20 suggests suggest VBZ cord-001249-awn9ayy6 152 21 that that IN cord-001249-awn9ayy6 152 22 NSDV NSDV NNP cord-001249-awn9ayy6 152 23 replication replication NN cord-001249-awn9ayy6 152 24 and/or and/or CC cord-001249-awn9ayy6 152 25 assembly assembly NN cord-001249-awn9ayy6 152 26 induces induce VBZ cord-001249-awn9ayy6 152 27 movement movement NN cord-001249-awn9ayy6 152 28 of of IN cord-001249-awn9ayy6 152 29 these these DT cord-001249-awn9ayy6 152 30 proteins protein NNS cord-001249-awn9ayy6 152 31 from from IN cord-001249-awn9ayy6 152 32 the the DT cord-001249-awn9ayy6 152 33 ER ER NNP cord-001249-awn9ayy6 152 34 to to IN cord-001249-awn9ayy6 152 35 the the DT cord-001249-awn9ayy6 152 36 cell cell NN cord-001249-awn9ayy6 152 37 surface surface NN cord-001249-awn9ayy6 152 38 . . . cord-001249-awn9ayy6 153 1 The the DT cord-001249-awn9ayy6 153 2 effect effect NN cord-001249-awn9ayy6 153 3 of of IN cord-001249-awn9ayy6 153 4 NSDV NSDV NNP cord-001249-awn9ayy6 153 5 replication replication NN cord-001249-awn9ayy6 153 6 on on IN cord-001249-awn9ayy6 153 7 PDI PDI NNP cord-001249-awn9ayy6 153 8 distribution distribution NN cord-001249-awn9ayy6 153 9 in in IN cord-001249-awn9ayy6 153 10 the the DT cord-001249-awn9ayy6 153 11 cell cell NN cord-001249-awn9ayy6 153 12 was be VBD cord-001249-awn9ayy6 153 13 also also RB cord-001249-awn9ayy6 153 14 tested test VBN cord-001249-awn9ayy6 153 15 in in IN cord-001249-awn9ayy6 153 16 other other JJ cord-001249-awn9ayy6 153 17 cell cell NN cord-001249-awn9ayy6 153 18 types type NNS cord-001249-awn9ayy6 153 19 . . . cord-001249-awn9ayy6 154 1 While while IN cord-001249-awn9ayy6 154 2 NSDVi NSDVi NNS cord-001249-awn9ayy6 154 3 grows grow VBZ cord-001249-awn9ayy6 154 4 relatively relatively RB cord-001249-awn9ayy6 154 5 poorly poorly RB cord-001249-awn9ayy6 154 6 in in IN cord-001249-awn9ayy6 154 7 a a DT cord-001249-awn9ayy6 154 8 caprine caprine NN cord-001249-awn9ayy6 154 9 endothelial endothelial JJ cord-001249-awn9ayy6 154 10 cell cell NN cord-001249-awn9ayy6 154 11 line line NN cord-001249-awn9ayy6 154 12 ( ( -LRB- cord-001249-awn9ayy6 154 13 CJE CJE NNP cord-001249-awn9ayy6 154 14 ) ) -RRB- cord-001249-awn9ayy6 154 15 , , , cord-001249-awn9ayy6 154 16 it -PRON- PRP cord-001249-awn9ayy6 154 17 still still RB cord-001249-awn9ayy6 154 18 induced induce VBD cord-001249-awn9ayy6 154 19 redistribution redistribution NN cord-001249-awn9ayy6 154 20 of of IN cord-001249-awn9ayy6 154 21 PDI PDI NNP cord-001249-awn9ayy6 154 22 from from IN cord-001249-awn9ayy6 154 23 the the DT cord-001249-awn9ayy6 154 24 ER ER NNP cord-001249-awn9ayy6 154 25 to to IN cord-001249-awn9ayy6 154 26 the the DT cord-001249-awn9ayy6 154 27 cell cell NN cord-001249-awn9ayy6 154 28 surface surface NN cord-001249-awn9ayy6 154 29 in in IN cord-001249-awn9ayy6 154 30 these these DT cord-001249-awn9ayy6 154 31 cells cell NNS cord-001249-awn9ayy6 154 32 ( ( -LRB- cord-001249-awn9ayy6 154 33 Figure figure NN cord-001249-awn9ayy6 154 34 5 5 CD cord-001249-awn9ayy6 154 35 ) ) -RRB- cord-001249-awn9ayy6 154 36 . . . cord-001249-awn9ayy6 155 1 Similar similar JJ cord-001249-awn9ayy6 155 2 changes change NNS cord-001249-awn9ayy6 155 3 to to IN cord-001249-awn9ayy6 155 4 the the DT cord-001249-awn9ayy6 155 5 distribution distribution NN cord-001249-awn9ayy6 155 6 of of IN cord-001249-awn9ayy6 155 7 PDI PDI NNP cord-001249-awn9ayy6 155 8 upon upon IN cord-001249-awn9ayy6 155 9 infection infection NN cord-001249-awn9ayy6 155 10 with with IN cord-001249-awn9ayy6 155 11 NSDV NSDV NNP cord-001249-awn9ayy6 155 12 ( ( -LRB- cord-001249-awn9ayy6 155 13 either either CC cord-001249-awn9ayy6 155 14 NSDVi NSDVi NNS cord-001249-awn9ayy6 155 15 or or CC cord-001249-awn9ayy6 155 16 NSDVu NSDVu NNP cord-001249-awn9ayy6 155 17 ) ) -RRB- cord-001249-awn9ayy6 155 18 were be VBD cord-001249-awn9ayy6 155 19 also also RB cord-001249-awn9ayy6 155 20 observed observe VBN cord-001249-awn9ayy6 155 21 in in IN cord-001249-awn9ayy6 155 22 A549 a549 JJ cord-001249-awn9ayy6 155 23 cells cell NNS cord-001249-awn9ayy6 155 24 and and CC cord-001249-awn9ayy6 155 25 in in IN cord-001249-awn9ayy6 155 26 a a DT cord-001249-awn9ayy6 155 27 bovine bovine JJ cord-001249-awn9ayy6 155 28 foetal foetal JJ cord-001249-awn9ayy6 155 29 endothelial endothelial JJ cord-001249-awn9ayy6 155 30 cell cell NN cord-001249-awn9ayy6 155 31 line line NN cord-001249-awn9ayy6 155 32 ( ( -LRB- cord-001249-awn9ayy6 155 33 BFA BFA NNP cord-001249-awn9ayy6 155 34 ) ) -RRB- cord-001249-awn9ayy6 155 35 ( ( -LRB- cord-001249-awn9ayy6 155 36 Figure figure NN cord-001249-awn9ayy6 155 37 S2 s2 NN cord-001249-awn9ayy6 155 38 ) ) -RRB- cord-001249-awn9ayy6 155 39 . . . cord-001249-awn9ayy6 156 1 This this DT cord-001249-awn9ayy6 156 2 confirmed confirm VBD cord-001249-awn9ayy6 156 3 that that IN cord-001249-awn9ayy6 156 4 redistribution redistribution NN cord-001249-awn9ayy6 156 5 of of IN cord-001249-awn9ayy6 156 6 PDI PDI NNP cord-001249-awn9ayy6 156 7 during during IN cord-001249-awn9ayy6 156 8 NSDV NSDV NNP cord-001249-awn9ayy6 156 9 infection infection NN cord-001249-awn9ayy6 156 10 is be VBZ cord-001249-awn9ayy6 156 11 not not RB cord-001249-awn9ayy6 156 12 Vero Vero NNP cord-001249-awn9ayy6 156 13 cell cell NN cord-001249-awn9ayy6 156 14 - - HYPH cord-001249-awn9ayy6 156 15 specific specific JJ cord-001249-awn9ayy6 156 16 , , , cord-001249-awn9ayy6 156 17 nor nor CC cord-001249-awn9ayy6 156 18 is be VBZ cord-001249-awn9ayy6 156 19 it -PRON- PRP cord-001249-awn9ayy6 156 20 specific specific JJ cord-001249-awn9ayy6 156 21 to to IN cord-001249-awn9ayy6 156 22 a a DT cord-001249-awn9ayy6 156 23 pathogenic pathogenic JJ cord-001249-awn9ayy6 156 24 or or CC cord-001249-awn9ayy6 156 25 apathogenic apathogenic JJ cord-001249-awn9ayy6 156 26 virus virus NN cord-001249-awn9ayy6 156 27 type type NN cord-001249-awn9ayy6 156 28 . . . cord-001249-awn9ayy6 157 1 NSDVi NSDVi NNS cord-001249-awn9ayy6 157 2 - - HYPH cord-001249-awn9ayy6 157 3 infected infect VBN cord-001249-awn9ayy6 157 4 cells cell NNS cord-001249-awn9ayy6 157 5 were be VBD cord-001249-awn9ayy6 157 6 studied study VBN cord-001249-awn9ayy6 157 7 at at IN cord-001249-awn9ayy6 157 8 different different JJ cord-001249-awn9ayy6 157 9 times time NNS cord-001249-awn9ayy6 157 10 post post JJ cord-001249-awn9ayy6 157 11 infection infection NN cord-001249-awn9ayy6 157 12 to to TO cord-001249-awn9ayy6 157 13 determine determine VB cord-001249-awn9ayy6 157 14 when when WRB cord-001249-awn9ayy6 157 15 during during IN cord-001249-awn9ayy6 157 16 infection infection NN cord-001249-awn9ayy6 157 17 changes change VBZ cord-001249-awn9ayy6 157 18 to to IN cord-001249-awn9ayy6 157 19 the the DT cord-001249-awn9ayy6 157 20 distribution distribution NN cord-001249-awn9ayy6 157 21 of of IN cord-001249-awn9ayy6 157 22 PDI PDI NNP cord-001249-awn9ayy6 157 23 appear appear VBP cord-001249-awn9ayy6 157 24 . . . cord-001249-awn9ayy6 158 1 Vero Vero NNP cord-001249-awn9ayy6 158 2 cells cell NNS cord-001249-awn9ayy6 158 3 were be VBD cord-001249-awn9ayy6 158 4 infected infect VBN cord-001249-awn9ayy6 158 5 at at IN cord-001249-awn9ayy6 158 6 high high JJ cord-001249-awn9ayy6 158 7 MOI MOI NNP cord-001249-awn9ayy6 158 8 and and CC cord-001249-awn9ayy6 158 9 fixed fix VBN cord-001249-awn9ayy6 158 10 at at IN cord-001249-awn9ayy6 158 11 0 0 CD cord-001249-awn9ayy6 158 12 , , , cord-001249-awn9ayy6 158 13 1 1 CD cord-001249-awn9ayy6 158 14 , , , cord-001249-awn9ayy6 158 15 2 2 CD cord-001249-awn9ayy6 158 16 , , , cord-001249-awn9ayy6 158 17 4 4 CD cord-001249-awn9ayy6 158 18 , , , cord-001249-awn9ayy6 158 19 8 8 CD cord-001249-awn9ayy6 158 20 , , , cord-001249-awn9ayy6 158 21 12 12 CD cord-001249-awn9ayy6 158 22 and and CC cord-001249-awn9ayy6 158 23 16 16 CD cord-001249-awn9ayy6 158 24 hours hour NNS cord-001249-awn9ayy6 158 25 post post NN cord-001249-awn9ayy6 158 26 infection infection NN cord-001249-awn9ayy6 158 27 ( ( -LRB- cord-001249-awn9ayy6 158 28 hpi hpi NNP cord-001249-awn9ayy6 158 29 ) ) -RRB- cord-001249-awn9ayy6 158 30 , , , cord-001249-awn9ayy6 158 31 after after IN cord-001249-awn9ayy6 158 32 which which WDT cord-001249-awn9ayy6 158 33 PDI PDI NNP cord-001249-awn9ayy6 158 34 was be VBD cord-001249-awn9ayy6 158 35 labelled label VBN cord-001249-awn9ayy6 158 36 in in IN cord-001249-awn9ayy6 158 37 the the DT cord-001249-awn9ayy6 158 38 ER ER NNP cord-001249-awn9ayy6 158 39 or or CC cord-001249-awn9ayy6 158 40 at at IN cord-001249-awn9ayy6 158 41 the the DT cord-001249-awn9ayy6 158 42 surface surface NN cord-001249-awn9ayy6 158 43 . . . cord-001249-awn9ayy6 159 1 The the DT cord-001249-awn9ayy6 159 2 first first JJ cord-001249-awn9ayy6 159 3 changes change NNS cord-001249-awn9ayy6 159 4 to to IN cord-001249-awn9ayy6 159 5 PDI PDI NNP cord-001249-awn9ayy6 159 6 distribution distribution NN cord-001249-awn9ayy6 159 7 could could MD cord-001249-awn9ayy6 159 8 be be VB cord-001249-awn9ayy6 159 9 observed observe VBN cord-001249-awn9ayy6 159 10 at at IN cord-001249-awn9ayy6 159 11 8 8 CD cord-001249-awn9ayy6 159 12 hpi hpi NNP cord-001249-awn9ayy6 159 13 , , , cord-001249-awn9ayy6 159 14 when when WRB cord-001249-awn9ayy6 159 15 a a DT cord-001249-awn9ayy6 159 16 few few JJ cord-001249-awn9ayy6 159 17 cells cell NNS cord-001249-awn9ayy6 159 18 showed show VBD cord-001249-awn9ayy6 159 19 a a DT cord-001249-awn9ayy6 159 20 reduced reduce VBN cord-001249-awn9ayy6 159 21 amount amount NN cord-001249-awn9ayy6 159 22 of of IN cord-001249-awn9ayy6 159 23 PDI PDI NNP cord-001249-awn9ayy6 159 24 in in IN cord-001249-awn9ayy6 159 25 the the DT cord-001249-awn9ayy6 159 26 ER ER NNP cord-001249-awn9ayy6 159 27 ( ( -LRB- cord-001249-awn9ayy6 159 28 observed observe VBN cord-001249-awn9ayy6 159 29 visually visually RB cord-001249-awn9ayy6 159 30 as as IN cord-001249-awn9ayy6 159 31 a a DT cord-001249-awn9ayy6 159 32 clearly clearly RB cord-001249-awn9ayy6 159 33 weaker weak JJR cord-001249-awn9ayy6 159 34 fluorescence fluorescence NN cord-001249-awn9ayy6 159 35 signal signal NN cord-001249-awn9ayy6 159 36 when when WRB cord-001249-awn9ayy6 159 37 compared compare VBN cord-001249-awn9ayy6 159 38 to to IN cord-001249-awn9ayy6 159 39 uninfected uninfected JJ cord-001249-awn9ayy6 159 40 cells cell NNS cord-001249-awn9ayy6 159 41 ) ) -RRB- cord-001249-awn9ayy6 159 42 and and CC cord-001249-awn9ayy6 159 43 vesicle vesicle NN cord-001249-awn9ayy6 159 44 - - HYPH cord-001249-awn9ayy6 159 45 like like JJ cord-001249-awn9ayy6 159 46 structures structure NNS cord-001249-awn9ayy6 159 47 containing contain VBG cord-001249-awn9ayy6 159 48 PDI PDI NNP cord-001249-awn9ayy6 159 49 could could MD cord-001249-awn9ayy6 159 50 be be VB cord-001249-awn9ayy6 159 51 noticed notice VBN cord-001249-awn9ayy6 160 1 ( ( -LRB- cord-001249-awn9ayy6 160 2 Figure figure NN cord-001249-awn9ayy6 160 3 6 6 CD cord-001249-awn9ayy6 160 4 a a DT cord-001249-awn9ayy6 160 5 - - HYPH cord-001249-awn9ayy6 160 6 c c NN cord-001249-awn9ayy6 160 7 ) ) -RRB- cord-001249-awn9ayy6 160 8 . . . cord-001249-awn9ayy6 161 1 At at IN cord-001249-awn9ayy6 161 2 12 12 CD cord-001249-awn9ayy6 161 3 hpi hpi NNP cord-001249-awn9ayy6 161 4 , , , cord-001249-awn9ayy6 161 5 several several JJ cord-001249-awn9ayy6 161 6 cells cell NNS cord-001249-awn9ayy6 161 7 which which WDT cord-001249-awn9ayy6 161 8 had have VBD cord-001249-awn9ayy6 161 9 completely completely RB cord-001249-awn9ayy6 161 10 lost lose VBN cord-001249-awn9ayy6 161 11 detectable detectable JJ cord-001249-awn9ayy6 161 12 PDI PDI NNP cord-001249-awn9ayy6 161 13 from from IN cord-001249-awn9ayy6 161 14 the the DT cord-001249-awn9ayy6 161 15 ER ER NNP cord-001249-awn9ayy6 161 16 were be VBD cord-001249-awn9ayy6 161 17 observed observe VBN cord-001249-awn9ayy6 161 18 ( ( -LRB- cord-001249-awn9ayy6 161 19 Figure figure NN cord-001249-awn9ayy6 161 20 6 6 CD cord-001249-awn9ayy6 161 21 d d NN cord-001249-awn9ayy6 161 22 - - HYPH cord-001249-awn9ayy6 161 23 f f NNP cord-001249-awn9ayy6 161 24 ) ) -RRB- cord-001249-awn9ayy6 161 25 and and CC cord-001249-awn9ayy6 161 26 at at IN cord-001249-awn9ayy6 161 27 16 16 CD cord-001249-awn9ayy6 161 28 hpi hpi NNP cord-001249-awn9ayy6 161 29 many many JJ cord-001249-awn9ayy6 161 30 cells cell NNS cord-001249-awn9ayy6 161 31 lacking lack VBG cord-001249-awn9ayy6 161 32 PDI PDI NNP cord-001249-awn9ayy6 161 33 were be VBD cord-001249-awn9ayy6 161 34 found find VBN cord-001249-awn9ayy6 161 35 ( ( -LRB- cord-001249-awn9ayy6 161 36 Figure figure NN cord-001249-awn9ayy6 161 37 6 6 CD cord-001249-awn9ayy6 161 38 g g NN cord-001249-awn9ayy6 161 39 - - : cord-001249-awn9ayy6 161 40 i i NNP cord-001249-awn9ayy6 161 41 ) ) -RRB- cord-001249-awn9ayy6 161 42 . . . cord-001249-awn9ayy6 162 1 The the DT cord-001249-awn9ayy6 162 2 first first JJ cord-001249-awn9ayy6 162 3 surface surface NN cord-001249-awn9ayy6 162 4 PDI PDI NNP cord-001249-awn9ayy6 162 5 was be VBD cord-001249-awn9ayy6 162 6 only only RB cord-001249-awn9ayy6 162 7 seen see VBN cord-001249-awn9ayy6 162 8 at at IN cord-001249-awn9ayy6 162 9 16 16 CD cord-001249-awn9ayy6 162 10 hpi hpi NNP cord-001249-awn9ayy6 162 11 ( ( -LRB- cord-001249-awn9ayy6 162 12 Figure Figure NNP cord-001249-awn9ayy6 162 13 6 6 CD cord-001249-awn9ayy6 162 14 j j NNP cord-001249-awn9ayy6 162 15 - - HYPH cord-001249-awn9ayy6 162 16 l l NNP cord-001249-awn9ayy6 162 17 ) ) -RRB- cord-001249-awn9ayy6 162 18 ; ; : cord-001249-awn9ayy6 162 19 however however RB cord-001249-awn9ayy6 162 20 , , , cord-001249-awn9ayy6 162 21 it -PRON- PRP cord-001249-awn9ayy6 162 22 is be VBZ cord-001249-awn9ayy6 162 23 possible possible JJ cord-001249-awn9ayy6 162 24 that that IN cord-001249-awn9ayy6 162 25 a a DT cord-001249-awn9ayy6 162 26 small small JJ cord-001249-awn9ayy6 162 27 increase increase NN cord-001249-awn9ayy6 162 28 of of IN cord-001249-awn9ayy6 162 29 surface surface NN cord-001249-awn9ayy6 162 30 PDI PDI NNP cord-001249-awn9ayy6 162 31 would would MD cord-001249-awn9ayy6 162 32 be be VB cord-001249-awn9ayy6 162 33 missed miss VBN cord-001249-awn9ayy6 162 34 at at IN cord-001249-awn9ayy6 162 35 earlier early JJR cord-001249-awn9ayy6 162 36 time time NN cord-001249-awn9ayy6 162 37 points point NNS cord-001249-awn9ayy6 162 38 due due JJ cord-001249-awn9ayy6 162 39 to to IN cord-001249-awn9ayy6 162 40 the the DT cord-001249-awn9ayy6 162 41 relatively relatively RB cord-001249-awn9ayy6 162 42 low low JJ cord-001249-awn9ayy6 162 43 sensitivity sensitivity NN cord-001249-awn9ayy6 162 44 of of IN cord-001249-awn9ayy6 162 45 confocal confocal JJ cord-001249-awn9ayy6 162 46 microscopy microscopy NNS cord-001249-awn9ayy6 162 47 . . . cord-001249-awn9ayy6 163 1 Similar similar JJ cord-001249-awn9ayy6 163 2 results result NNS cord-001249-awn9ayy6 163 3 were be VBD cord-001249-awn9ayy6 163 4 obtained obtain VBN cord-001249-awn9ayy6 163 5 for for IN cord-001249-awn9ayy6 163 6 ERp57 ERp57 NNS cord-001249-awn9ayy6 163 7 ( ( -LRB- cord-001249-awn9ayy6 163 8 Figure figure NN cord-001249-awn9ayy6 163 9 S3 S3 NNP cord-001249-awn9ayy6 163 10 ) ) -RRB- cord-001249-awn9ayy6 163 11 . . . cord-001249-awn9ayy6 164 1 Interestingly interestingly RB cord-001249-awn9ayy6 164 2 , , , cord-001249-awn9ayy6 164 3 despite despite IN cord-001249-awn9ayy6 164 4 synchronising synchronise VBG cord-001249-awn9ayy6 164 5 infection infection NN cord-001249-awn9ayy6 164 6 by by IN cord-001249-awn9ayy6 164 7 washing wash VBG cord-001249-awn9ayy6 164 8 away away RB cord-001249-awn9ayy6 164 9 unbound unbound JJ cord-001249-awn9ayy6 164 10 virus virus NN cord-001249-awn9ayy6 164 11 1 1 CD cord-001249-awn9ayy6 164 12 h h NN cord-001249-awn9ayy6 164 13 post post NN cord-001249-awn9ayy6 164 14 inoculation inoculation NN cord-001249-awn9ayy6 164 15 , , , cord-001249-awn9ayy6 164 16 it -PRON- PRP cord-001249-awn9ayy6 164 17 was be VBD cord-001249-awn9ayy6 164 18 observed observe VBN cord-001249-awn9ayy6 164 19 that that IN cord-001249-awn9ayy6 164 20 infection infection NN cord-001249-awn9ayy6 164 21 had have VBD cord-001249-awn9ayy6 164 22 not not RB cord-001249-awn9ayy6 164 23 progressed progress VBN cord-001249-awn9ayy6 164 24 to to IN cord-001249-awn9ayy6 164 25 the the DT cord-001249-awn9ayy6 164 26 same same JJ cord-001249-awn9ayy6 164 27 degree degree NN cord-001249-awn9ayy6 164 28 in in IN cord-001249-awn9ayy6 164 29 all all DT cord-001249-awn9ayy6 164 30 infected infected JJ cord-001249-awn9ayy6 164 31 cells cell NNS cord-001249-awn9ayy6 164 32 , , , cord-001249-awn9ayy6 164 33 so so IN cord-001249-awn9ayy6 164 34 that that IN cord-001249-awn9ayy6 164 35 , , , cord-001249-awn9ayy6 164 36 at at IN cord-001249-awn9ayy6 164 37 8 8 CD cord-001249-awn9ayy6 164 38 hpi hpi NNP cord-001249-awn9ayy6 164 39 , , , cord-001249-awn9ayy6 164 40 only only RB cord-001249-awn9ayy6 164 41 a a DT cord-001249-awn9ayy6 164 42 few few JJ cord-001249-awn9ayy6 164 43 cells cell NNS cord-001249-awn9ayy6 164 44 showed show VBD cord-001249-awn9ayy6 164 45 detectable detectable JJ cord-001249-awn9ayy6 164 46 viral viral JJ cord-001249-awn9ayy6 164 47 N n CD cord-001249-awn9ayy6 164 48 protein protein NN cord-001249-awn9ayy6 164 49 , , , cord-001249-awn9ayy6 164 50 while while IN cord-001249-awn9ayy6 164 51 at at IN cord-001249-awn9ayy6 164 52 12 12 CD cord-001249-awn9ayy6 164 53 hpi hpi NNP cord-001249-awn9ayy6 164 54 , , , cord-001249-awn9ayy6 164 55 at at IN cord-001249-awn9ayy6 164 56 which which WDT cord-001249-awn9ayy6 164 57 time time NN cord-001249-awn9ayy6 164 58 progeny progeny NN cord-001249-awn9ayy6 164 59 virus virus NN cord-001249-awn9ayy6 164 60 can can MD cord-001249-awn9ayy6 164 61 be be VB cord-001249-awn9ayy6 164 62 detected detect VBN cord-001249-awn9ayy6 164 63 [ [ -LRB- cord-001249-awn9ayy6 164 64 29 29 CD cord-001249-awn9ayy6 164 65 ] ] -RRB- cord-001249-awn9ayy6 164 66 , , , cord-001249-awn9ayy6 164 67 cells cell NNS cord-001249-awn9ayy6 164 68 with with IN cord-001249-awn9ayy6 164 69 very very RB cord-001249-awn9ayy6 164 70 different different JJ cord-001249-awn9ayy6 164 71 amounts amount NNS cord-001249-awn9ayy6 164 72 of of IN cord-001249-awn9ayy6 164 73 N n CD cord-001249-awn9ayy6 164 74 were be VBD cord-001249-awn9ayy6 164 75 observed observe VBN cord-001249-awn9ayy6 165 1 ( ( -LRB- cord-001249-awn9ayy6 165 2 Figure figure NN cord-001249-awn9ayy6 165 3 6 6 CD cord-001249-awn9ayy6 165 4 ) ) -RRB- cord-001249-awn9ayy6 165 5 . . . cord-001249-awn9ayy6 166 1 This this DT cord-001249-awn9ayy6 166 2 apparent apparent JJ cord-001249-awn9ayy6 166 3 variation variation NN cord-001249-awn9ayy6 166 4 in in IN cord-001249-awn9ayy6 166 5 the the DT cord-001249-awn9ayy6 166 6 extent extent NN cord-001249-awn9ayy6 166 7 of of IN cord-001249-awn9ayy6 166 8 virus virus NN cord-001249-awn9ayy6 166 9 replication replication NN cord-001249-awn9ayy6 166 10 in in IN cord-001249-awn9ayy6 166 11 individual individual JJ cord-001249-awn9ayy6 166 12 cells cell NNS cord-001249-awn9ayy6 166 13 from from IN cord-001249-awn9ayy6 166 14 the the DT cord-001249-awn9ayy6 166 15 same same JJ cord-001249-awn9ayy6 166 16 sample sample NN cord-001249-awn9ayy6 166 17 may may MD cord-001249-awn9ayy6 166 18 be be VB cord-001249-awn9ayy6 166 19 due due JJ cord-001249-awn9ayy6 166 20 to to IN cord-001249-awn9ayy6 166 21 variation variation NN cord-001249-awn9ayy6 166 22 in in IN cord-001249-awn9ayy6 166 23 the the DT cord-001249-awn9ayy6 166 24 virions virion NNS cord-001249-awn9ayy6 166 25 themselves -PRON- PRP cord-001249-awn9ayy6 166 26 ( ( -LRB- cord-001249-awn9ayy6 166 27 e.g. e.g. JJ cord-001249-awn9ayy6 166 28 individual individual JJ cord-001249-awn9ayy6 166 29 virus virus NN cord-001249-awn9ayy6 166 30 particles particle NNS cord-001249-awn9ayy6 166 31 having have VBG cord-001249-awn9ayy6 166 32 different different JJ cord-001249-awn9ayy6 166 33 numbers number NNS cord-001249-awn9ayy6 166 34 of of IN cord-001249-awn9ayy6 166 35 copies copy NNS cord-001249-awn9ayy6 166 36 of of IN cord-001249-awn9ayy6 166 37 each each DT cord-001249-awn9ayy6 166 38 segment segment NN cord-001249-awn9ayy6 166 39 ) ) -RRB- cord-001249-awn9ayy6 166 40 or or CC cord-001249-awn9ayy6 166 41 due due IN cord-001249-awn9ayy6 166 42 to to IN cord-001249-awn9ayy6 166 43 the the DT cord-001249-awn9ayy6 166 44 virus virus NN cord-001249-awn9ayy6 166 45 replicating replicate VBG cord-001249-awn9ayy6 166 46 with with IN cord-001249-awn9ayy6 166 47 different different JJ cord-001249-awn9ayy6 166 48 rates rate NNS cord-001249-awn9ayy6 166 49 in in IN cord-001249-awn9ayy6 166 50 different different JJ cord-001249-awn9ayy6 166 51 cells cell NNS cord-001249-awn9ayy6 166 52 , , , cord-001249-awn9ayy6 166 53 possibly possibly RB cord-001249-awn9ayy6 166 54 due due IN cord-001249-awn9ayy6 166 55 to to IN cord-001249-awn9ayy6 166 56 some some DT cord-001249-awn9ayy6 166 57 effect effect NN cord-001249-awn9ayy6 166 58 of of IN cord-001249-awn9ayy6 166 59 cell cell NN cord-001249-awn9ayy6 166 60 cycle cycle NN cord-001249-awn9ayy6 166 61 , , , cord-001249-awn9ayy6 166 62 as as IN cord-001249-awn9ayy6 166 63 the the DT cord-001249-awn9ayy6 166 64 cell cell NN cord-001249-awn9ayy6 166 65 cycle cycle NN cord-001249-awn9ayy6 166 66 of of IN cord-001249-awn9ayy6 166 67 the the DT cord-001249-awn9ayy6 166 68 cells cell NNS cord-001249-awn9ayy6 166 69 used use VBN cord-001249-awn9ayy6 166 70 in in IN cord-001249-awn9ayy6 166 71 this this DT cord-001249-awn9ayy6 166 72 experiment experiment NN cord-001249-awn9ayy6 166 73 was be VBD cord-001249-awn9ayy6 166 74 not not RB cord-001249-awn9ayy6 166 75 synchronised synchronised JJ cord-001249-awn9ayy6 166 76 . . . cord-001249-awn9ayy6 167 1 We -PRON- PRP cord-001249-awn9ayy6 167 2 also also RB cord-001249-awn9ayy6 167 3 observed observe VBD cord-001249-awn9ayy6 167 4 that that IN cord-001249-awn9ayy6 167 5 some some DT cord-001249-awn9ayy6 167 6 NSDVi NSDVi NNS cord-001249-awn9ayy6 167 7 - - HYPH cord-001249-awn9ayy6 167 8 infected infect VBN cord-001249-awn9ayy6 167 9 cells cell NNS cord-001249-awn9ayy6 167 10 , , , cord-001249-awn9ayy6 167 11 despite despite IN cord-001249-awn9ayy6 167 12 containing contain VBG cord-001249-awn9ayy6 167 13 large large JJ cord-001249-awn9ayy6 167 14 amount amount NN cord-001249-awn9ayy6 167 15 of of IN cord-001249-awn9ayy6 167 16 N n CD cord-001249-awn9ayy6 167 17 protein protein NN cord-001249-awn9ayy6 167 18 , , , cord-001249-awn9ayy6 167 19 showed show VBD cord-001249-awn9ayy6 167 20 very very RB cord-001249-awn9ayy6 167 21 little little JJ cord-001249-awn9ayy6 167 22 or or CC cord-001249-awn9ayy6 167 23 no no UH cord-001249-awn9ayy6 167 24 PreGn pregn NN cord-001249-awn9ayy6 167 25 ( ( -LRB- cord-001249-awn9ayy6 167 26 Figure Figure NNP cord-001249-awn9ayy6 167 27 S4 S4 NNP cord-001249-awn9ayy6 167 28 ) ) -RRB- cord-001249-awn9ayy6 167 29 , , , cord-001249-awn9ayy6 167 30 which which WDT cord-001249-awn9ayy6 167 31 could could MD cord-001249-awn9ayy6 167 32 explain explain VB cord-001249-awn9ayy6 167 33 why why WRB cord-001249-awn9ayy6 167 34 not not RB cord-001249-awn9ayy6 167 35 all all DT cord-001249-awn9ayy6 167 36 cells cell NNS cord-001249-awn9ayy6 167 37 judged judge VBN cord-001249-awn9ayy6 167 38 to to TO cord-001249-awn9ayy6 167 39 be be VB cord-001249-awn9ayy6 167 40 infected infect VBN cord-001249-awn9ayy6 167 41 because because IN cord-001249-awn9ayy6 167 42 of of IN cord-001249-awn9ayy6 167 43 the the DT cord-001249-awn9ayy6 167 44 presence presence NN cord-001249-awn9ayy6 167 45 of of IN cord-001249-awn9ayy6 167 46 viral viral JJ cord-001249-awn9ayy6 167 47 N n CD cord-001249-awn9ayy6 167 48 protein protein NN cord-001249-awn9ayy6 167 49 had have VBD cord-001249-awn9ayy6 167 50 lost lose VBN cord-001249-awn9ayy6 167 51 PDI PDI NNP cord-001249-awn9ayy6 167 52 from from IN cord-001249-awn9ayy6 167 53 their -PRON- PRP$ cord-001249-awn9ayy6 167 54 ER er NN cord-001249-awn9ayy6 167 55 or or CC cord-001249-awn9ayy6 167 56 showed show VBD cord-001249-awn9ayy6 167 57 surface surface NN cord-001249-awn9ayy6 167 58 PDI PDI NNP cord-001249-awn9ayy6 167 59 expression expression NN cord-001249-awn9ayy6 167 60 . . . cord-001249-awn9ayy6 168 1 Differential differential JJ cord-001249-awn9ayy6 168 2 expression expression NN cord-001249-awn9ayy6 168 3 of of IN cord-001249-awn9ayy6 168 4 the the DT cord-001249-awn9ayy6 168 5 N N NNP cord-001249-awn9ayy6 168 6 and and CC cord-001249-awn9ayy6 168 7 PreGn pregn PRP cord-001249-awn9ayy6 168 8 proteins protein VBZ cord-001249-awn9ayy6 168 9 in in IN cord-001249-awn9ayy6 168 10 NSDV NSDV NNP cord-001249-awn9ayy6 168 11 - - HYPH cord-001249-awn9ayy6 168 12 infected infect VBN cord-001249-awn9ayy6 168 13 cells cell NNS cord-001249-awn9ayy6 168 14 may may MD cord-001249-awn9ayy6 168 15 reflect reflect VB cord-001249-awn9ayy6 168 16 the the DT cord-001249-awn9ayy6 168 17 apparent apparent JJ cord-001249-awn9ayy6 168 18 non non JJ cord-001249-awn9ayy6 168 19 - - JJ cord-001249-awn9ayy6 168 20 equimolar equimolar JJ cord-001249-awn9ayy6 168 21 amount amount NN cord-001249-awn9ayy6 168 22 of of IN cord-001249-awn9ayy6 168 23 the the DT cord-001249-awn9ayy6 168 24 three three CD cord-001249-awn9ayy6 168 25 viral viral JJ cord-001249-awn9ayy6 168 26 RNA RNA NNP cord-001249-awn9ayy6 168 27 segments segment NNS cord-001249-awn9ayy6 168 28 found find VBD cord-001249-awn9ayy6 168 29 previously previously RB cord-001249-awn9ayy6 168 30 in in IN cord-001249-awn9ayy6 168 31 number number NN cord-001249-awn9ayy6 168 32 of of IN cord-001249-awn9ayy6 168 33 bunyaviruses bunyavirus NNS cord-001249-awn9ayy6 168 34 [ [ -LRB- cord-001249-awn9ayy6 168 35 40 40 CD cord-001249-awn9ayy6 168 36 , , , cord-001249-awn9ayy6 168 37 [ [ -LRB- cord-001249-awn9ayy6 168 38 71 71 CD cord-001249-awn9ayy6 168 39 ] ] -RRB- cord-001249-awn9ayy6 168 40 [ [ -LRB- cord-001249-awn9ayy6 168 41 72 72 CD cord-001249-awn9ayy6 168 42 ] ] -RRB- cord-001249-awn9ayy6 168 43 [ [ -LRB- cord-001249-awn9ayy6 168 44 73 73 CD cord-001249-awn9ayy6 168 45 ] ] -RRB- cord-001249-awn9ayy6 168 46 . . . cord-001249-awn9ayy6 169 1 Since since IN cord-001249-awn9ayy6 169 2 PDI PDI NNP cord-001249-awn9ayy6 169 3 is be VBZ cord-001249-awn9ayy6 169 4 a a DT cord-001249-awn9ayy6 169 5 soluble soluble JJ cord-001249-awn9ayy6 169 6 protein protein NN cord-001249-awn9ayy6 169 7 which which WDT cord-001249-awn9ayy6 169 8 is be VBZ cord-001249-awn9ayy6 169 9 thought think VBN cord-001249-awn9ayy6 169 10 to to TO cord-001249-awn9ayy6 169 11 be be VB cord-001249-awn9ayy6 169 12 retained retain VBN cord-001249-awn9ayy6 169 13 at at IN cord-001249-awn9ayy6 169 14 the the DT cord-001249-awn9ayy6 169 15 cell cell NN cord-001249-awn9ayy6 169 16 surface surface NN cord-001249-awn9ayy6 169 17 through through IN cord-001249-awn9ayy6 169 18 non non JJ cord-001249-awn9ayy6 169 19 - - JJ cord-001249-awn9ayy6 169 20 covalent covalent JJ cord-001249-awn9ayy6 169 21 interactions interaction NNS cord-001249-awn9ayy6 169 22 [ [ -LRB- cord-001249-awn9ayy6 169 23 70 70 CD cord-001249-awn9ayy6 169 24 ] ] -RRB- cord-001249-awn9ayy6 169 25 , , , cord-001249-awn9ayy6 169 26 and and CC cord-001249-awn9ayy6 169 27 the the DT cord-001249-awn9ayy6 169 28 secretion secretion NN cord-001249-awn9ayy6 169 29 of of IN cord-001249-awn9ayy6 169 30 PDI PDI NNP cord-001249-awn9ayy6 169 31 has have VBZ cord-001249-awn9ayy6 169 32 been be VBN cord-001249-awn9ayy6 169 33 observed observe VBN cord-001249-awn9ayy6 169 34 from from IN cord-001249-awn9ayy6 169 35 a a DT cord-001249-awn9ayy6 169 36 number number NN cord-001249-awn9ayy6 169 37 of of IN cord-001249-awn9ayy6 169 38 cell cell NN cord-001249-awn9ayy6 169 39 types type NNS cord-001249-awn9ayy6 169 40 [ [ -LRB- cord-001249-awn9ayy6 169 41 70 70 CD cord-001249-awn9ayy6 169 42 , , , cord-001249-awn9ayy6 169 43 74 74 CD cord-001249-awn9ayy6 169 44 ] ] -RRB- cord-001249-awn9ayy6 169 45 , , , cord-001249-awn9ayy6 169 46 we -PRON- PRP cord-001249-awn9ayy6 169 47 investigated investigate VBD cord-001249-awn9ayy6 169 48 whether whether IN cord-001249-awn9ayy6 169 49 PDI PDI NNP cord-001249-awn9ayy6 169 50 and and CC cord-001249-awn9ayy6 169 51 ERp57 ERp57 NNS cord-001249-awn9ayy6 169 52 not not RB cord-001249-awn9ayy6 169 53 only only RB cord-001249-awn9ayy6 169 54 appear appear VB cord-001249-awn9ayy6 169 55 at at IN cord-001249-awn9ayy6 169 56 the the DT cord-001249-awn9ayy6 169 57 surface surface NN cord-001249-awn9ayy6 169 58 of of IN cord-001249-awn9ayy6 169 59 NSDV NSDV NNP cord-001249-awn9ayy6 169 60 - - HYPH cord-001249-awn9ayy6 169 61 infected infect VBN cord-001249-awn9ayy6 169 62 cells cell NNS cord-001249-awn9ayy6 169 63 but but CC cord-001249-awn9ayy6 169 64 are be VBP cord-001249-awn9ayy6 169 65 also also RB cord-001249-awn9ayy6 169 66 secreted secrete VBN cord-001249-awn9ayy6 169 67 from from IN cord-001249-awn9ayy6 169 68 these these DT cord-001249-awn9ayy6 169 69 cells cell NNS cord-001249-awn9ayy6 169 70 . . . cord-001249-awn9ayy6 170 1 Vero Vero NNP cord-001249-awn9ayy6 170 2 cells cell NNS cord-001249-awn9ayy6 170 3 were be VBD cord-001249-awn9ayy6 170 4 again again RB cord-001249-awn9ayy6 170 5 infected infect VBN cord-001249-awn9ayy6 170 6 with with IN cord-001249-awn9ayy6 170 7 NSDVi NSDVi NNS cord-001249-awn9ayy6 170 8 and and CC cord-001249-awn9ayy6 170 9 culture culture NN cord-001249-awn9ayy6 170 10 supernatants supernatant NNS cord-001249-awn9ayy6 170 11 ( ( -LRB- cord-001249-awn9ayy6 170 12 i.e. i.e. FW cord-001249-awn9ayy6 170 13 the the DT cord-001249-awn9ayy6 170 14 cell cell NN cord-001249-awn9ayy6 170 15 medium medium NN cord-001249-awn9ayy6 170 16 ) ) -RRB- cord-001249-awn9ayy6 170 17 and and CC cord-001249-awn9ayy6 170 18 the the DT cord-001249-awn9ayy6 170 19 cells cell NNS cord-001249-awn9ayy6 170 20 themselves -PRON- PRP cord-001249-awn9ayy6 170 21 were be VBD cord-001249-awn9ayy6 170 22 harvested harvest VBN cord-001249-awn9ayy6 170 23 , , , cord-001249-awn9ayy6 170 24 and and CC cord-001249-awn9ayy6 170 25 proteins protein NNS cord-001249-awn9ayy6 170 26 from from IN cord-001249-awn9ayy6 170 27 supernatants supernatant NNS cord-001249-awn9ayy6 170 28 and/or and/or CC cord-001249-awn9ayy6 170 29 cell cell NN cord-001249-awn9ayy6 170 30 lysates lysate NNS cord-001249-awn9ayy6 170 31 were be VBD cord-001249-awn9ayy6 170 32 analysed analyse VBN cord-001249-awn9ayy6 170 33 by by IN cord-001249-awn9ayy6 170 34 Western western JJ cord-001249-awn9ayy6 170 35 blotting blotting NN cord-001249-awn9ayy6 170 36 using use VBG cord-001249-awn9ayy6 170 37 specific specific JJ cord-001249-awn9ayy6 170 38 antibodies antibody NNS cord-001249-awn9ayy6 170 39 . . . cord-001249-awn9ayy6 171 1 Before before IN cord-001249-awn9ayy6 171 2 harvesting harvesting NN cord-001249-awn9ayy6 171 3 , , , cord-001249-awn9ayy6 171 4 cells cell NNS cord-001249-awn9ayy6 171 5 were be VBD cord-001249-awn9ayy6 171 6 checked check VBN cord-001249-awn9ayy6 171 7 by by IN cord-001249-awn9ayy6 171 8 light light JJ cord-001249-awn9ayy6 171 9 microscopy microscopy NNS cord-001249-awn9ayy6 171 10 for for IN cord-001249-awn9ayy6 171 11 the the DT cord-001249-awn9ayy6 171 12 appearance appearance NN cord-001249-awn9ayy6 171 13 of of IN cord-001249-awn9ayy6 171 14 any any DT cord-001249-awn9ayy6 171 15 cytopathic cytopathic JJ cord-001249-awn9ayy6 171 16 effect effect NN cord-001249-awn9ayy6 171 17 ( ( -LRB- cord-001249-awn9ayy6 171 18 CPE CPE NNP cord-001249-awn9ayy6 171 19 ) ) -RRB- cord-001249-awn9ayy6 171 20 , , , cord-001249-awn9ayy6 171 21 to to TO cord-001249-awn9ayy6 171 22 ensure ensure VB cord-001249-awn9ayy6 171 23 that that IN cord-001249-awn9ayy6 171 24 only only RB cord-001249-awn9ayy6 171 25 the the DT cord-001249-awn9ayy6 171 26 medium medium NN cord-001249-awn9ayy6 171 27 from from IN cord-001249-awn9ayy6 171 28 cells cell NNS cord-001249-awn9ayy6 171 29 which which WDT cord-001249-awn9ayy6 171 30 appeared appear VBD cord-001249-awn9ayy6 171 31 healthy healthy JJ cord-001249-awn9ayy6 171 32 was be VBD cord-001249-awn9ayy6 171 33 used use VBN cord-001249-awn9ayy6 171 34 in in IN cord-001249-awn9ayy6 171 35 this this DT cord-001249-awn9ayy6 171 36 study study NN cord-001249-awn9ayy6 171 37 . . . cord-001249-awn9ayy6 172 1 After after IN cord-001249-awn9ayy6 172 2 48 48 CD cord-001249-awn9ayy6 172 3 h h NN cord-001249-awn9ayy6 172 4 , , , cord-001249-awn9ayy6 172 5 in in IN cord-001249-awn9ayy6 172 6 cells cell NNS cord-001249-awn9ayy6 172 7 infected infect VBN cord-001249-awn9ayy6 172 8 with with IN cord-001249-awn9ayy6 172 9 NSDVi NSDVi NNS cord-001249-awn9ayy6 172 10 at at IN cord-001249-awn9ayy6 172 11 a a DT cord-001249-awn9ayy6 172 12 low low JJ cord-001249-awn9ayy6 172 13 MOI MOI NNP cord-001249-awn9ayy6 172 14 , , , cord-001249-awn9ayy6 172 15 no no DT cord-001249-awn9ayy6 172 16 CPE CPE NNP cord-001249-awn9ayy6 172 17 was be VBD cord-001249-awn9ayy6 172 18 observed observe VBN cord-001249-awn9ayy6 172 19 in in IN cord-001249-awn9ayy6 172 20 the the DT cord-001249-awn9ayy6 172 21 culture culture NN cord-001249-awn9ayy6 172 22 , , , cord-001249-awn9ayy6 172 23 but but CC cord-001249-awn9ayy6 172 24 a a DT cord-001249-awn9ayy6 172 25 clear clear JJ cord-001249-awn9ayy6 172 26 increase increase NN cord-001249-awn9ayy6 172 27 in in IN cord-001249-awn9ayy6 172 28 levels level NNS cord-001249-awn9ayy6 172 29 of of IN cord-001249-awn9ayy6 172 30 PDI PDI NNP cord-001249-awn9ayy6 172 31 and and CC cord-001249-awn9ayy6 172 32 ERp57 ERp57 NNPS cord-001249-awn9ayy6 172 33 was be VBD cord-001249-awn9ayy6 172 34 detected detect VBN cord-001249-awn9ayy6 172 35 in in IN cord-001249-awn9ayy6 172 36 the the DT cord-001249-awn9ayy6 172 37 medium medium NN cord-001249-awn9ayy6 172 38 . . . cord-001249-awn9ayy6 173 1 These these DT cord-001249-awn9ayy6 173 2 proteins protein NNS cord-001249-awn9ayy6 173 3 were be VBD cord-001249-awn9ayy6 173 4 found find VBN cord-001249-awn9ayy6 173 5 in in IN cord-001249-awn9ayy6 173 6 the the DT cord-001249-awn9ayy6 173 7 medium medium NN cord-001249-awn9ayy6 173 8 from from IN cord-001249-awn9ayy6 173 9 infected infected JJ cord-001249-awn9ayy6 173 10 cells cell NNS cord-001249-awn9ayy6 173 11 but but CC cord-001249-awn9ayy6 173 12 not not RB cord-001249-awn9ayy6 173 13 from from IN cord-001249-awn9ayy6 173 14 uninfected uninfected JJ cord-001249-awn9ayy6 173 15 cells cell NNS cord-001249-awn9ayy6 173 16 ( ( -LRB- cord-001249-awn9ayy6 173 17 Figure Figure NNP cord-001249-awn9ayy6 173 18 7 7 CD cord-001249-awn9ayy6 173 19 ) ) -RRB- cord-001249-awn9ayy6 173 20 , , , cord-001249-awn9ayy6 173 21 while while IN cord-001249-awn9ayy6 173 22 cellular cellular JJ cord-001249-awn9ayy6 173 23 proteins protein NNS cord-001249-awn9ayy6 173 24 such such JJ cord-001249-awn9ayy6 173 25 as as IN cord-001249-awn9ayy6 173 26 tubulin tubulin JJ cord-001249-awn9ayy6 173 27 or or CC cord-001249-awn9ayy6 173 28 calnexin calnexin NN cord-001249-awn9ayy6 173 29 were be VBD cord-001249-awn9ayy6 173 30 not not RB cord-001249-awn9ayy6 173 31 found find VBN cord-001249-awn9ayy6 173 32 in in IN cord-001249-awn9ayy6 173 33 the the DT cord-001249-awn9ayy6 173 34 medium medium NN cord-001249-awn9ayy6 173 35 ( ( -LRB- cord-001249-awn9ayy6 173 36 Figure Figure NNP cord-001249-awn9ayy6 173 37 7 7 CD cord-001249-awn9ayy6 173 38 ) ) -RRB- cord-001249-awn9ayy6 173 39 , , , cord-001249-awn9ayy6 173 40 indicating indicate VBG cord-001249-awn9ayy6 173 41 that that IN cord-001249-awn9ayy6 173 42 the the DT cord-001249-awn9ayy6 173 43 PDI PDI NNP cord-001249-awn9ayy6 173 44 , , , cord-001249-awn9ayy6 173 45 ERp57 ERp57 NNS cord-001249-awn9ayy6 173 46 and and CC cord-001249-awn9ayy6 173 47 NSDV NSDV NNP cord-001249-awn9ayy6 173 48 N N NNP cord-001249-awn9ayy6 173 49 protein protein NN cord-001249-awn9ayy6 173 50 were be VBD cord-001249-awn9ayy6 173 51 not not RB cord-001249-awn9ayy6 173 52 simply simply RB cord-001249-awn9ayy6 173 53 coming come VBG cord-001249-awn9ayy6 173 54 from from IN cord-001249-awn9ayy6 173 55 ruptured ruptured JJ cord-001249-awn9ayy6 173 56 cells cell NNS cord-001249-awn9ayy6 173 57 . . . cord-001249-awn9ayy6 174 1 The the DT cord-001249-awn9ayy6 174 2 NSDV NSDV NNP cord-001249-awn9ayy6 174 3 glycoproteins glycoprotein NNS cord-001249-awn9ayy6 174 4 mature mature VBP cord-001249-awn9ayy6 174 5 in in IN cord-001249-awn9ayy6 174 6 the the DT cord-001249-awn9ayy6 174 7 ER ER NNP cord-001249-awn9ayy6 174 8 and and CC cord-001249-awn9ayy6 174 9 Golgi Golgi NNP cord-001249-awn9ayy6 174 10 and and CC cord-001249-awn9ayy6 174 11 their -PRON- PRP$ cord-001249-awn9ayy6 174 12 synthesis synthesis NN cord-001249-awn9ayy6 174 13 , , , cord-001249-awn9ayy6 174 14 like like IN cord-001249-awn9ayy6 174 15 that that DT cord-001249-awn9ayy6 174 16 of of IN cord-001249-awn9ayy6 174 17 most most JJS cord-001249-awn9ayy6 174 18 glycoproteins glycoprotein NNS cord-001249-awn9ayy6 174 19 , , , cord-001249-awn9ayy6 174 20 will will MD cord-001249-awn9ayy6 174 21 involve involve VB cord-001249-awn9ayy6 174 22 the the DT cord-001249-awn9ayy6 174 23 ER ER NNP cord-001249-awn9ayy6 174 24 chaperones chaperone NNS cord-001249-awn9ayy6 174 25 . . . cord-001249-awn9ayy6 175 1 It -PRON- PRP cord-001249-awn9ayy6 175 2 is be VBZ cord-001249-awn9ayy6 175 3 therefore therefore RB cord-001249-awn9ayy6 175 4 possible possible JJ cord-001249-awn9ayy6 175 5 that that IN cord-001249-awn9ayy6 175 6 the the DT cord-001249-awn9ayy6 175 7 viral viral JJ cord-001249-awn9ayy6 175 8 glycoproteins glycoprotein NNS cord-001249-awn9ayy6 175 9 may may MD cord-001249-awn9ayy6 175 10 be be VB cord-001249-awn9ayy6 175 11 involved involve VBN cord-001249-awn9ayy6 175 12 in in IN cord-001249-awn9ayy6 175 13 the the DT cord-001249-awn9ayy6 175 14 redistribution redistribution NN cord-001249-awn9ayy6 175 15 of of IN cord-001249-awn9ayy6 175 16 PDI PDI NNP cord-001249-awn9ayy6 175 17 / / SYM cord-001249-awn9ayy6 175 18 ERp57 ERp57 NNS cord-001249-awn9ayy6 175 19 in in IN cord-001249-awn9ayy6 175 20 infected infected JJ cord-001249-awn9ayy6 175 21 cells cell NNS cord-001249-awn9ayy6 175 22 . . . cord-001249-awn9ayy6 176 1 In in IN cord-001249-awn9ayy6 176 2 order order NN cord-001249-awn9ayy6 176 3 to to TO cord-001249-awn9ayy6 176 4 visualise visualise VB cord-001249-awn9ayy6 176 5 this this DT cord-001249-awn9ayy6 176 6 directly directly RB cord-001249-awn9ayy6 176 7 , , , cord-001249-awn9ayy6 176 8 we -PRON- PRP cord-001249-awn9ayy6 176 9 labelled label VBD cord-001249-awn9ayy6 176 10 infected infected JJ cord-001249-awn9ayy6 176 11 cells cell NNS cord-001249-awn9ayy6 176 12 with with IN cord-001249-awn9ayy6 176 13 rabbit rabbit NN cord-001249-awn9ayy6 176 14 anti anti NNS cord-001249-awn9ayy6 176 15 - - NNS cord-001249-awn9ayy6 176 16 ERp57 erp57 JJ cord-001249-awn9ayy6 176 17 and and CC cord-001249-awn9ayy6 176 18 mouse mouse NN cord-001249-awn9ayy6 176 19 anti anti NNP cord-001249-awn9ayy6 176 20 - - NNP cord-001249-awn9ayy6 176 21 PreGn PreGn NNP cord-001249-awn9ayy6 176 22 . . . cord-001249-awn9ayy6 177 1 It -PRON- PRP cord-001249-awn9ayy6 177 2 was be VBD cord-001249-awn9ayy6 177 3 observed observe VBN cord-001249-awn9ayy6 177 4 that that IN cord-001249-awn9ayy6 177 5 ERp57 ERp57 NNS cord-001249-awn9ayy6 177 6 is be VBZ cord-001249-awn9ayy6 177 7 redistributed redistribute VBN cord-001249-awn9ayy6 177 8 in in IN cord-001249-awn9ayy6 177 9 infected infected JJ cord-001249-awn9ayy6 177 10 cells cell NNS cord-001249-awn9ayy6 177 11 so so IN cord-001249-awn9ayy6 177 12 that that IN cord-001249-awn9ayy6 177 13 it -PRON- PRP cord-001249-awn9ayy6 177 14 is be VBZ cord-001249-awn9ayy6 177 15 essentially essentially RB cord-001249-awn9ayy6 177 16 all all DT cord-001249-awn9ayy6 177 17 in in IN cord-001249-awn9ayy6 177 18 the the DT cord-001249-awn9ayy6 177 19 specific specific JJ cord-001249-awn9ayy6 177 20 areas area NNS cord-001249-awn9ayy6 177 21 occupied occupy VBN cord-001249-awn9ayy6 177 22 by by IN cord-001249-awn9ayy6 177 23 viral viral JJ cord-001249-awn9ayy6 177 24 glycoproteins glycoprotein NNS cord-001249-awn9ayy6 177 25 ( ( -LRB- cord-001249-awn9ayy6 177 26 Figure figure NN cord-001249-awn9ayy6 177 27 8.A 8.a CD cord-001249-awn9ayy6 177 28 ) ) -RRB- cord-001249-awn9ayy6 177 29 , , , cord-001249-awn9ayy6 177 30 suggesting suggest VBG cord-001249-awn9ayy6 177 31 that that IN cord-001249-awn9ayy6 177 32 these these DT cord-001249-awn9ayy6 177 33 chaperones chaperone NNS cord-001249-awn9ayy6 177 34 may may MD cord-001249-awn9ayy6 177 35 be be VB cord-001249-awn9ayy6 177 36 recruited recruit VBN cord-001249-awn9ayy6 177 37 to to IN cord-001249-awn9ayy6 177 38 the the DT cord-001249-awn9ayy6 177 39 parts part NNS cord-001249-awn9ayy6 177 40 of of IN cord-001249-awn9ayy6 177 41 the the DT cord-001249-awn9ayy6 177 42 ER ER NNP cord-001249-awn9ayy6 177 43 involved involve VBN cord-001249-awn9ayy6 177 44 in in IN cord-001249-awn9ayy6 177 45 synthesising synthesise VBG cord-001249-awn9ayy6 177 46 the the DT cord-001249-awn9ayy6 177 47 viral viral JJ cord-001249-awn9ayy6 177 48 glycoproteins glycoprotein NNS cord-001249-awn9ayy6 177 49 . . . cord-001249-awn9ayy6 178 1 Although although IN cord-001249-awn9ayy6 178 2 we -PRON- PRP cord-001249-awn9ayy6 178 3 could could MD cord-001249-awn9ayy6 178 4 not not RB cord-001249-awn9ayy6 178 5 carry carry VB cord-001249-awn9ayy6 178 6 out out RP cord-001249-awn9ayy6 178 7 a a DT cord-001249-awn9ayy6 178 8 similar similar JJ cord-001249-awn9ayy6 178 9 study study NN cord-001249-awn9ayy6 178 10 of of IN cord-001249-awn9ayy6 178 11 PDI PDI NNP cord-001249-awn9ayy6 178 12 / / SYM cord-001249-awn9ayy6 178 13 PreGn PreGn NNP cord-001249-awn9ayy6 178 14 colocalisation colocalisation NN cord-001249-awn9ayy6 178 15 ( ( -LRB- cord-001249-awn9ayy6 178 16 no no DT cord-001249-awn9ayy6 178 17 good good JJ cord-001249-awn9ayy6 178 18 rabbit rabbit NN cord-001249-awn9ayy6 178 19 anti anti NNP cord-001249-awn9ayy6 178 20 - - NNP cord-001249-awn9ayy6 178 21 PDI PDI NNP cord-001249-awn9ayy6 178 22 was be VBD cord-001249-awn9ayy6 178 23 found find VBN cord-001249-awn9ayy6 178 24 ) ) -RRB- cord-001249-awn9ayy6 178 25 , , , cord-001249-awn9ayy6 178 26 a a DT cord-001249-awn9ayy6 178 27 similar similar JJ cord-001249-awn9ayy6 178 28 association association NN cord-001249-awn9ayy6 178 29 of of IN cord-001249-awn9ayy6 178 30 PDI PDI NNP cord-001249-awn9ayy6 178 31 and and CC cord-001249-awn9ayy6 178 32 viral viral JJ cord-001249-awn9ayy6 178 33 glycoprotein glycoprotein NN cord-001249-awn9ayy6 178 34 is be VBZ cord-001249-awn9ayy6 178 35 strongly strongly RB cord-001249-awn9ayy6 178 36 suggested suggest VBN cord-001249-awn9ayy6 178 37 by by IN cord-001249-awn9ayy6 178 38 studies study NNS cord-001249-awn9ayy6 178 39 in in IN cord-001249-awn9ayy6 178 40 which which WDT cord-001249-awn9ayy6 178 41 we -PRON- PRP cord-001249-awn9ayy6 178 42 lysed lyse VBD cord-001249-awn9ayy6 178 43 infected infected JJ cord-001249-awn9ayy6 178 44 cells cell NNS cord-001249-awn9ayy6 178 45 and and CC cord-001249-awn9ayy6 178 46 immunoextracted immunoextracte VBD cord-001249-awn9ayy6 178 47 the the DT cord-001249-awn9ayy6 178 48 lysates lysate NNS cord-001249-awn9ayy6 178 49 using use VBG cord-001249-awn9ayy6 178 50 mouse mouse NN cord-001249-awn9ayy6 178 51 anti anti NNP cord-001249-awn9ayy6 178 52 - - NNP cord-001249-awn9ayy6 178 53 PDI pdi JJ cord-001249-awn9ayy6 178 54 ( ( -LRB- cord-001249-awn9ayy6 178 55 clone clone JJ cord-001249-awn9ayy6 178 56 RL90 RL90 NNP cord-001249-awn9ayy6 178 57 ) ) -RRB- cord-001249-awn9ayy6 178 58 antibody antibody NN cord-001249-awn9ayy6 178 59 . . . cord-001249-awn9ayy6 179 1 We -PRON- PRP cord-001249-awn9ayy6 179 2 found find VBD cord-001249-awn9ayy6 179 3 PreGn pregn PRP cord-001249-awn9ayy6 179 4 , , , cord-001249-awn9ayy6 179 5 but but CC cord-001249-awn9ayy6 179 6 not not RB cord-001249-awn9ayy6 179 7 N n CD cord-001249-awn9ayy6 179 8 , , , cord-001249-awn9ayy6 179 9 among among IN cord-001249-awn9ayy6 179 10 proteins protein NNS cord-001249-awn9ayy6 179 11 co co VBD cord-001249-awn9ayy6 179 12 - - VBD cord-001249-awn9ayy6 179 13 precipitated precipitate VBN cord-001249-awn9ayy6 179 14 by by IN cord-001249-awn9ayy6 179 15 anti anti JJ cord-001249-awn9ayy6 179 16 - - JJ cord-001249-awn9ayy6 179 17 PDI pdi JJ cord-001249-awn9ayy6 179 18 antibody antibody NN cord-001249-awn9ayy6 179 19 from from IN cord-001249-awn9ayy6 179 20 infected infected JJ cord-001249-awn9ayy6 179 21 cells cell NNS cord-001249-awn9ayy6 179 22 ( ( -LRB- cord-001249-awn9ayy6 179 23 Figure figure NN cord-001249-awn9ayy6 179 24 8 8 CD cord-001249-awn9ayy6 179 25 .B .B NFP cord-001249-awn9ayy6 179 26 ) ) -RRB- cord-001249-awn9ayy6 179 27 , , , cord-001249-awn9ayy6 179 28 indicating indicate VBG cord-001249-awn9ayy6 179 29 that that IN cord-001249-awn9ayy6 179 30 PDI PDI NNP cord-001249-awn9ayy6 179 31 appears appear VBZ cord-001249-awn9ayy6 179 32 to to TO cord-001249-awn9ayy6 179 33 interact interact VB cord-001249-awn9ayy6 179 34 , , , cord-001249-awn9ayy6 179 35 directly directly RB cord-001249-awn9ayy6 179 36 or or CC cord-001249-awn9ayy6 179 37 indirectly indirectly RB cord-001249-awn9ayy6 179 38 , , , cord-001249-awn9ayy6 179 39 with with IN cord-001249-awn9ayy6 179 40 PreGn pregn PRP cord-001249-awn9ayy6 179 41 and and CC cord-001249-awn9ayy6 179 42 possibly possibly RB cord-001249-awn9ayy6 179 43 other other JJ cord-001249-awn9ayy6 179 44 of of IN cord-001249-awn9ayy6 179 45 the the DT cord-001249-awn9ayy6 179 46 viral viral JJ cord-001249-awn9ayy6 179 47 glycoproteins glycoprotein NNS cord-001249-awn9ayy6 179 48 in in IN cord-001249-awn9ayy6 179 49 NSDV NSDV NNP cord-001249-awn9ayy6 179 50 - - HYPH cord-001249-awn9ayy6 179 51 infected infect VBN cord-001249-awn9ayy6 179 52 cells cell NNS cord-001249-awn9ayy6 180 1 ( ( -LRB- cord-001249-awn9ayy6 180 2 Figure figure NN cord-001249-awn9ayy6 180 3 8 8 CD cord-001249-awn9ayy6 180 4 .B .B NFP cord-001249-awn9ayy6 180 5 ) ) -RRB- cord-001249-awn9ayy6 181 1 ( ( -LRB- cord-001249-awn9ayy6 181 2 note note VB cord-001249-awn9ayy6 181 3 that that DT cord-001249-awn9ayy6 181 4 reverse reverse JJ cord-001249-awn9ayy6 181 5 immunoprecipitation immunoprecipitation NN cord-001249-awn9ayy6 181 6 with with IN cord-001249-awn9ayy6 181 7 anti anti JJ cord-001249-awn9ayy6 181 8 - - JJ cord-001249-awn9ayy6 181 9 PreGn pregn PRP cord-001249-awn9ayy6 181 10 was be VBD cord-001249-awn9ayy6 181 11 not not RB cord-001249-awn9ayy6 181 12 Figure figure NN cord-001249-awn9ayy6 181 13 1 1 CD cord-001249-awn9ayy6 181 14 except except IN cord-001249-awn9ayy6 181 15 that that DT cord-001249-awn9ayy6 181 16 , , , cord-001249-awn9ayy6 181 17 after after IN cord-001249-awn9ayy6 181 18 fixing fix VBG cord-001249-awn9ayy6 181 19 with with IN cord-001249-awn9ayy6 181 20 3 3 CD cord-001249-awn9ayy6 181 21 % % NN cord-001249-awn9ayy6 181 22 PFA PFA NNP cord-001249-awn9ayy6 181 23 , , , cord-001249-awn9ayy6 181 24 cells cell NNS cord-001249-awn9ayy6 181 25 were be VBD cord-001249-awn9ayy6 181 26 labelled label VBN cord-001249-awn9ayy6 181 27 with with IN cord-001249-awn9ayy6 181 28 mouse mouse NN cord-001249-awn9ayy6 181 29 anti anti JJ cord-001249-awn9ayy6 181 30 - - NNP cord-001249-awn9ayy6 181 31 PDI pdi JJ cord-001249-awn9ayy6 182 1 ( ( -LRB- cord-001249-awn9ayy6 182 2 clone clone NN cord-001249-awn9ayy6 182 3 1D3 1d3 CD cord-001249-awn9ayy6 182 4 ; ; : cord-001249-awn9ayy6 182 5 a a DT cord-001249-awn9ayy6 182 6 - - HYPH cord-001249-awn9ayy6 182 7 c c NN cord-001249-awn9ayy6 182 8 ) ) -RRB- cord-001249-awn9ayy6 182 9 , , , cord-001249-awn9ayy6 182 10 mouse mouse NN cord-001249-awn9ayy6 182 11 anti anti NNP cord-001249-awn9ayy6 182 12 - - NNP cord-001249-awn9ayy6 182 13 PDI pdi JJ cord-001249-awn9ayy6 182 14 ( ( -LRB- cord-001249-awn9ayy6 182 15 clone clone JJ cord-001249-awn9ayy6 182 16 RL90 RL90 NNP cord-001249-awn9ayy6 182 17 ; ; : cord-001249-awn9ayy6 182 18 d d NN cord-001249-awn9ayy6 182 19 - - HYPH cord-001249-awn9ayy6 182 20 f f NNP cord-001249-awn9ayy6 182 21 ) ) -RRB- cord-001249-awn9ayy6 182 22 or or CC cord-001249-awn9ayy6 182 23 rabbit rabbit NN cord-001249-awn9ayy6 182 24 anti anti NNS cord-001249-awn9ayy6 182 25 - - NNS cord-001249-awn9ayy6 182 26 ERp57 erp57 JJ cord-001249-awn9ayy6 182 27 ( ( -LRB- cord-001249-awn9ayy6 182 28 g g NN cord-001249-awn9ayy6 182 29 - - : cord-001249-awn9ayy6 182 30 i i NNP cord-001249-awn9ayy6 182 31 ) ) -RRB- cord-001249-awn9ayy6 182 32 . . . cord-001249-awn9ayy6 183 1 Then then RB cord-001249-awn9ayy6 183 2 cells cell NNS cord-001249-awn9ayy6 183 3 were be VBD cord-001249-awn9ayy6 183 4 again again RB cord-001249-awn9ayy6 183 5 fixed fix VBN cord-001249-awn9ayy6 183 6 with with IN cord-001249-awn9ayy6 183 7 3 3 CD cord-001249-awn9ayy6 183 8 % % NN cord-001249-awn9ayy6 183 9 PFA PFA NNP cord-001249-awn9ayy6 183 10 , , , cord-001249-awn9ayy6 183 11 opened open VBD cord-001249-awn9ayy6 183 12 with with IN cord-001249-awn9ayy6 183 13 ice ice NN cord-001249-awn9ayy6 183 14 - - HYPH cord-001249-awn9ayy6 183 15 cold cold JJ cord-001249-awn9ayy6 183 16 methanol methanol NN cord-001249-awn9ayy6 183 17 , , , cord-001249-awn9ayy6 183 18 and and CC cord-001249-awn9ayy6 183 19 stained stain VBN cord-001249-awn9ayy6 183 20 with with IN cord-001249-awn9ayy6 183 21 rabbit rabbit NN cord-001249-awn9ayy6 183 22 antiserum antiserum NN cord-001249-awn9ayy6 183 23 against against IN cord-001249-awn9ayy6 183 24 the the DT cord-001249-awn9ayy6 183 25 viral viral JJ cord-001249-awn9ayy6 183 26 N n CD cord-001249-awn9ayy6 183 27 protein protein NN cord-001249-awn9ayy6 183 28 ( ( -LRB- cord-001249-awn9ayy6 183 29 a a FW cord-001249-awn9ayy6 183 30 - - HYPH cord-001249-awn9ayy6 183 31 f f NN cord-001249-awn9ayy6 183 32 ) ) -RRB- cord-001249-awn9ayy6 183 33 or or CC cord-001249-awn9ayy6 183 34 with with IN cord-001249-awn9ayy6 183 35 mouse mouse NN cord-001249-awn9ayy6 183 36 anti anti JJ cord-001249-awn9ayy6 183 37 - - JJ cord-001249-awn9ayy6 183 38 PreGn pregn JJ cord-001249-awn9ayy6 183 39 antibody antibody NN cord-001249-awn9ayy6 183 40 ( ( -LRB- cord-001249-awn9ayy6 183 41 g g NN cord-001249-awn9ayy6 183 42 - - : cord-001249-awn9ayy6 183 43 i i NNP cord-001249-awn9ayy6 183 44 ) ) -RRB- cord-001249-awn9ayy6 183 45 . . . cord-001249-awn9ayy6 184 1 Proteins protein NNS cord-001249-awn9ayy6 184 2 were be VBD cord-001249-awn9ayy6 184 3 visualised visualise VBN cord-001249-awn9ayy6 184 4 by by IN cord-001249-awn9ayy6 184 5 co co NN cord-001249-awn9ayy6 184 6 - - NN cord-001249-awn9ayy6 184 7 staining staining NN cord-001249-awn9ayy6 184 8 with with IN cord-001249-awn9ayy6 184 9 Alexa Alexa NNP cord-001249-awn9ayy6 184 10 Fluor Fluor NNP cord-001249-awn9ayy6 184 11 488 488 CD cord-001249-awn9ayy6 184 12 goat goat NN cord-001249-awn9ayy6 184 13 anti anti JJ cord-001249-awn9ayy6 184 14 - - JJ cord-001249-awn9ayy6 184 15 mouse mouse JJ cord-001249-awn9ayy6 184 16 IgG IgG NNP cord-001249-awn9ayy6 184 17 ( ( -LRB- cord-001249-awn9ayy6 184 18 green green NNP cord-001249-awn9ayy6 184 19 ) ) -RRB- cord-001249-awn9ayy6 184 20 and and CC cord-001249-awn9ayy6 184 21 Alexa Alexa NNP cord-001249-awn9ayy6 184 22 Fluor Fluor NNP cord-001249-awn9ayy6 184 23 568 568 CD cord-001249-awn9ayy6 184 24 goat goat NN cord-001249-awn9ayy6 184 25 anti anti JJ cord-001249-awn9ayy6 184 26 - - JJ cord-001249-awn9ayy6 184 27 rabbit rabbit JJ cord-001249-awn9ayy6 184 28 IgG igg NN cord-001249-awn9ayy6 184 29 ( ( -LRB- cord-001249-awn9ayy6 184 30 red red NNP cord-001249-awn9ayy6 184 31 ) ) -RRB- cord-001249-awn9ayy6 184 32 . . . cord-001249-awn9ayy6 185 1 B. B. NNP cord-001249-awn9ayy6 185 2 Vero Vero NNP cord-001249-awn9ayy6 185 3 cells cell NNS cord-001249-awn9ayy6 185 4 were be VBD cord-001249-awn9ayy6 185 5 infected infect VBN cord-001249-awn9ayy6 185 6 with with IN cord-001249-awn9ayy6 185 7 the the DT cord-001249-awn9ayy6 185 8 NSDVi NSDVi NNS cord-001249-awn9ayy6 185 9 isolate isolate VBP cord-001249-awn9ayy6 185 10 at at IN cord-001249-awn9ayy6 185 11 a a DT cord-001249-awn9ayy6 185 12 MOI MOI NNP cord-001249-awn9ayy6 185 13 of of IN cord-001249-awn9ayy6 185 14 6 6 CD cord-001249-awn9ayy6 185 15 TCID TCID NNP cord-001249-awn9ayy6 185 16 50 50 CD cord-001249-awn9ayy6 185 17 or or CC cord-001249-awn9ayy6 185 18 left leave VBN cord-001249-awn9ayy6 185 19 uninfected uninfected JJ cord-001249-awn9ayy6 185 20 . . . cord-001249-awn9ayy6 186 1 After after IN cord-001249-awn9ayy6 186 2 16 16 CD cord-001249-awn9ayy6 186 3 h h NN cord-001249-awn9ayy6 186 4 , , , cord-001249-awn9ayy6 186 5 cells cell NNS cord-001249-awn9ayy6 186 6 were be VBD cord-001249-awn9ayy6 186 7 fixed fix VBN cord-001249-awn9ayy6 186 8 using use VBG cord-001249-awn9ayy6 186 9 3 3 CD cord-001249-awn9ayy6 186 10 % % NN cord-001249-awn9ayy6 186 11 PFA PFA NNP cord-001249-awn9ayy6 186 12 and and CC cord-001249-awn9ayy6 186 13 left leave VBD cord-001249-awn9ayy6 186 14 nonpermeabilised nonpermeabilised JJ cord-001249-awn9ayy6 186 15 . . . cord-001249-awn9ayy6 187 1 Cells cell NNS cord-001249-awn9ayy6 187 2 were be VBD cord-001249-awn9ayy6 187 3 incubated incubate VBN cord-001249-awn9ayy6 187 4 with with IN cord-001249-awn9ayy6 187 5 mouse mouse NN cord-001249-awn9ayy6 187 6 anti anti JJ cord-001249-awn9ayy6 187 7 - - NNP cord-001249-awn9ayy6 187 8 PDI pdi JJ cord-001249-awn9ayy6 187 9 ( ( -LRB- cord-001249-awn9ayy6 187 10 clone clone NN cord-001249-awn9ayy6 187 11 1D3 1d3 CD cord-001249-awn9ayy6 187 12 ) ) -RRB- cord-001249-awn9ayy6 187 13 antibody antibody NN cord-001249-awn9ayy6 187 14 followed follow VBN cord-001249-awn9ayy6 187 15 by by IN cord-001249-awn9ayy6 187 16 Alexa Alexa NNP cord-001249-awn9ayy6 187 17 Fluor Fluor NNP cord-001249-awn9ayy6 187 18 488 488 CD cord-001249-awn9ayy6 187 19 goat goat NN cord-001249-awn9ayy6 187 20 anti anti JJ cord-001249-awn9ayy6 187 21 - - JJ cord-001249-awn9ayy6 187 22 mouse mouse JJ cord-001249-awn9ayy6 187 23 IgG igg NN cord-001249-awn9ayy6 187 24 ( ( -LRB- cord-001249-awn9ayy6 187 25 green green NNP cord-001249-awn9ayy6 187 26 ) ) -RRB- cord-001249-awn9ayy6 187 27 . . . cord-001249-awn9ayy6 188 1 Nuclei nucleus NNS cord-001249-awn9ayy6 188 2 were be VBD cord-001249-awn9ayy6 188 3 counterstained counterstaine VBN cord-001249-awn9ayy6 188 4 using use VBG cord-001249-awn9ayy6 188 5 DAPI DAPI NNP cord-001249-awn9ayy6 188 6 ( ( -LRB- cord-001249-awn9ayy6 188 7 blue blue JJ cord-001249-awn9ayy6 188 8 ) ) -RRB- cord-001249-awn9ayy6 188 9 . . . cord-001249-awn9ayy6 189 1 Bars bar NNS cord-001249-awn9ayy6 189 2 correspond correspond VBP cord-001249-awn9ayy6 189 3 to to IN cord-001249-awn9ayy6 189 4 40 40 CD cord-001249-awn9ayy6 189 5 mm mm NNS cord-001249-awn9ayy6 189 6 . . . cord-001249-awn9ayy6 190 1 doi:10.1371 doi:10.1371 NNP cord-001249-awn9ayy6 191 1 /journal.pone.0094656.g004 /journal.pone.0094656.g004 . cord-001249-awn9ayy6 191 2 Export Export NNP cord-001249-awn9ayy6 191 3 of of IN cord-001249-awn9ayy6 191 4 ER ER NNP cord-001249-awn9ayy6 191 5 Proteins Proteins NNP cord-001249-awn9ayy6 191 6 in in IN cord-001249-awn9ayy6 191 7 Nairovirus Nairovirus NNP cord-001249-awn9ayy6 191 8 - - HYPH cord-001249-awn9ayy6 191 9 Infected Infected NNP cord-001249-awn9ayy6 191 10 Cells Cells NNPS cord-001249-awn9ayy6 191 11 PLOS PLOS NNP cord-001249-awn9ayy6 191 12 ONE one CD cord-001249-awn9ayy6 191 13 | | : cord-001249-awn9ayy6 191 14 www.plosone.org www.plosone.org RBS cord-001249-awn9ayy6 191 15 possible possible JJ cord-001249-awn9ayy6 191 16 since since IN cord-001249-awn9ayy6 191 17 the the DT cord-001249-awn9ayy6 191 18 monoclonal monoclonal JJ cord-001249-awn9ayy6 191 19 antibody antibody NN cord-001249-awn9ayy6 191 20 in in IN cord-001249-awn9ayy6 191 21 question question NN cord-001249-awn9ayy6 191 22 appears appear VBZ cord-001249-awn9ayy6 191 23 to to TO cord-001249-awn9ayy6 191 24 recognise recognise VB cord-001249-awn9ayy6 191 25 only only RB cord-001249-awn9ayy6 191 26 denatured denature VBN cord-001249-awn9ayy6 191 27 PreGn PreGn NNP cord-001249-awn9ayy6 191 28 ) ) -RRB- cord-001249-awn9ayy6 191 29 . . . cord-001249-awn9ayy6 192 1 We -PRON- PRP cord-001249-awn9ayy6 192 2 constructed construct VBD cord-001249-awn9ayy6 192 3 plasmids plasmid NNS cord-001249-awn9ayy6 192 4 expressing express VBG cord-001249-awn9ayy6 192 5 NSDV NSDV NNP cord-001249-awn9ayy6 192 6 glycoproteins glycoprotein NNS cord-001249-awn9ayy6 192 7 PreGn pregn PRP cord-001249-awn9ayy6 192 8 , , , cord-001249-awn9ayy6 192 9 NS NS NNP cord-001249-awn9ayy6 192 10 M M NNP cord-001249-awn9ayy6 192 11 or or CC cord-001249-awn9ayy6 192 12 PreGc PreGc NNPS cord-001249-awn9ayy6 192 13 to to TO cord-001249-awn9ayy6 192 14 determine determine VB cord-001249-awn9ayy6 192 15 which which WDT cord-001249-awn9ayy6 192 16 glycoprotein(s glycoprotein(s NNP cord-001249-awn9ayy6 192 17 ) ) -RRB- cord-001249-awn9ayy6 192 18 were be VBD cord-001249-awn9ayy6 192 19 responsible responsible JJ cord-001249-awn9ayy6 192 20 for for IN cord-001249-awn9ayy6 192 21 the the DT cord-001249-awn9ayy6 192 22 redistribution redistribution NN cord-001249-awn9ayy6 192 23 of of IN cord-001249-awn9ayy6 192 24 PDI PDI NNP cord-001249-awn9ayy6 192 25 / / SYM cord-001249-awn9ayy6 192 26 ERp57 ERp57 NNS cord-001249-awn9ayy6 192 27 . . . cord-001249-awn9ayy6 193 1 In in IN cord-001249-awn9ayy6 193 2 silico silico NN cord-001249-awn9ayy6 193 3 analysis analysis NN cord-001249-awn9ayy6 193 4 of of IN cord-001249-awn9ayy6 193 5 the the DT cord-001249-awn9ayy6 193 6 protein protein NN cord-001249-awn9ayy6 193 7 sequence sequence NN cord-001249-awn9ayy6 193 8 encoded encode VBN cord-001249-awn9ayy6 193 9 by by IN cord-001249-awn9ayy6 193 10 the the DT cord-001249-awn9ayy6 193 11 NSDV NSDV NNP cord-001249-awn9ayy6 193 12 M M NNP cord-001249-awn9ayy6 193 13 segment segment NN cord-001249-awn9ayy6 193 14 suggested suggest VBD cord-001249-awn9ayy6 193 15 that that IN cord-001249-awn9ayy6 193 16 the the DT cord-001249-awn9ayy6 193 17 NSDV NSDV NNP cord-001249-awn9ayy6 193 18 glycoproteins glycoprotein NNS cord-001249-awn9ayy6 193 19 have have VBP cord-001249-awn9ayy6 193 20 a a DT cord-001249-awn9ayy6 193 21 similar similar JJ cord-001249-awn9ayy6 193 22 organisational organisational JJ cord-001249-awn9ayy6 193 23 arrangement arrangement NN cord-001249-awn9ayy6 193 24 to to IN cord-001249-awn9ayy6 193 25 that that DT cord-001249-awn9ayy6 193 26 described describe VBN cord-001249-awn9ayy6 193 27 for for IN cord-001249-awn9ayy6 193 28 CCHFV CCHFV NNP cord-001249-awn9ayy6 193 29 [ [ -LRB- cord-001249-awn9ayy6 193 30 50 50 CD cord-001249-awn9ayy6 193 31 ] ] -RRB- cord-001249-awn9ayy6 193 32 , , , cord-001249-awn9ayy6 193 33 although although IN cord-001249-awn9ayy6 193 34 with with IN cord-001249-awn9ayy6 193 35 some some DT cord-001249-awn9ayy6 193 36 differences difference NNS cord-001249-awn9ayy6 193 37 in in IN cord-001249-awn9ayy6 193 38 the the DT cord-001249-awn9ayy6 193 39 exact exact JJ cord-001249-awn9ayy6 193 40 lengths length NNS cord-001249-awn9ayy6 193 41 of of IN cord-001249-awn9ayy6 193 42 the the DT cord-001249-awn9ayy6 193 43 different different JJ cord-001249-awn9ayy6 193 44 proteins protein NNS cord-001249-awn9ayy6 193 45 . . . cord-001249-awn9ayy6 194 1 Transmembrane Transmembrane NNP cord-001249-awn9ayy6 194 2 ( ( -LRB- cord-001249-awn9ayy6 194 3 TM TM NNP cord-001249-awn9ayy6 194 4 ) ) -RRB- cord-001249-awn9ayy6 194 5 regions region NNS cord-001249-awn9ayy6 194 6 and and CC cord-001249-awn9ayy6 194 7 signalase signalase NN cord-001249-awn9ayy6 194 8 cleavage cleavage NN cord-001249-awn9ayy6 194 9 sites site NNS cord-001249-awn9ayy6 194 10 were be VBD cord-001249-awn9ayy6 194 11 predicted predict VBN cord-001249-awn9ayy6 194 12 using use VBG cord-001249-awn9ayy6 194 13 TMHMM TMHMM NNP cord-001249-awn9ayy6 194 14 Server Server NNP cord-001249-awn9ayy6 194 15 v. v. CC cord-001249-awn9ayy6 194 16 2.0 2.0 CD cord-001249-awn9ayy6 194 17 and and CC cord-001249-awn9ayy6 194 18 SignalP signalp VB cord-001249-awn9ayy6 194 19 4.0 4.0 CD cord-001249-awn9ayy6 194 20 Server server NN cord-001249-awn9ayy6 194 21 [ [ -LRB- cord-001249-awn9ayy6 194 22 75 75 CD cord-001249-awn9ayy6 194 23 ] ] -RRB- cord-001249-awn9ayy6 194 24 respectively respectively RB cord-001249-awn9ayy6 194 25 ; ; : cord-001249-awn9ayy6 194 26 six six CD cord-001249-awn9ayy6 194 27 hydrophobic hydrophobic JJ cord-001249-awn9ayy6 194 28 regions region NNS cord-001249-awn9ayy6 194 29 TM TM NNP cord-001249-awn9ayy6 194 30 0 0 NFP cord-001249-awn9ayy6 194 31 ( ( -LRB- cord-001249-awn9ayy6 194 32 N n CD cord-001249-awn9ayy6 194 33 - - HYPH cord-001249-awn9ayy6 194 34 terminal terminal NN cord-001249-awn9ayy6 194 35 ) ) -RRB- cord-001249-awn9ayy6 194 36 , , , cord-001249-awn9ayy6 194 37 TM TM NNP cord-001249-awn9ayy6 194 38 1 1 CD cord-001249-awn9ayy6 194 39 ( ( -LRB- cord-001249-awn9ayy6 194 40 631 631 CD cord-001249-awn9ayy6 194 41 - - SYM cord-001249-awn9ayy6 194 42 653 653 CD cord-001249-awn9ayy6 194 43 aa aa NNP cord-001249-awn9ayy6 194 44 ) ) -RRB- cord-001249-awn9ayy6 194 45 , , , cord-001249-awn9ayy6 194 46 TM TM NNP cord-001249-awn9ayy6 194 47 2 2 CD cord-001249-awn9ayy6 194 48 ( ( -LRB- cord-001249-awn9ayy6 194 49 751 751 CD cord-001249-awn9ayy6 194 50 - - HYPH cord-001249-awn9ayy6 194 51 773 773 CD cord-001249-awn9ayy6 194 52 aa aa NNP cord-001249-awn9ayy6 194 53 ) ) -RRB- cord-001249-awn9ayy6 194 54 , , , cord-001249-awn9ayy6 194 55 TM TM NNP cord-001249-awn9ayy6 194 56 3 3 CD cord-001249-awn9ayy6 194 57 ( ( -LRB- cord-001249-awn9ayy6 194 58 786 786 CD cord-001249-awn9ayy6 194 59 - - SYM cord-001249-awn9ayy6 194 60 803 803 CD cord-001249-awn9ayy6 194 61 aa aa NN cord-001249-awn9ayy6 194 62 ) ) -RRB- cord-001249-awn9ayy6 194 63 , , , cord-001249-awn9ayy6 194 64 TM TM NNP cord-001249-awn9ayy6 194 65 4 4 CD cord-001249-awn9ayy6 194 66 ( ( -LRB- cord-001249-awn9ayy6 194 67 907 907 CD cord-001249-awn9ayy6 194 68 - - SYM cord-001249-awn9ayy6 194 69 928 928 CD cord-001249-awn9ayy6 194 70 aa aa NNP cord-001249-awn9ayy6 194 71 ) ) -RRB- cord-001249-awn9ayy6 194 72 and and CC cord-001249-awn9ayy6 194 73 TM TM NNP cord-001249-awn9ayy6 194 74 5 5 CD cord-001249-awn9ayy6 194 75 ( ( -LRB- cord-001249-awn9ayy6 194 76 1524 1524 CD cord-001249-awn9ayy6 194 77 - - SYM cord-001249-awn9ayy6 194 78 1546 1546 CD cord-001249-awn9ayy6 194 79 aa aa NNP cord-001249-awn9ayy6 194 80 ) ) -RRB- cord-001249-awn9ayy6 194 81 were be VBD cord-001249-awn9ayy6 194 82 predicted predict VBN cord-001249-awn9ayy6 194 83 with with IN cord-001249-awn9ayy6 194 84 signalase signalase NN cord-001249-awn9ayy6 194 85 cleavage cleavage NN cord-001249-awn9ayy6 194 86 sites site NNS cord-001249-awn9ayy6 194 87 just just RB cord-001249-awn9ayy6 194 88 after after IN cord-001249-awn9ayy6 194 89 the the DT cord-001249-awn9ayy6 194 90 hydrophobic hydrophobic JJ cord-001249-awn9ayy6 194 91 sequences sequence NNS cord-001249-awn9ayy6 194 92 of of IN cord-001249-awn9ayy6 194 93 TM TM NNP cord-001249-awn9ayy6 194 94 0 0 CD cord-001249-awn9ayy6 194 95 ( ( -LRB- cord-001249-awn9ayy6 194 96 19Q20 19q20 CD cord-001249-awn9ayy6 194 97 aa aa NNP cord-001249-awn9ayy6 194 98 ) ) -RRB- cord-001249-awn9ayy6 194 99 , , , cord-001249-awn9ayy6 194 100 TM TM NNP cord-001249-awn9ayy6 194 101 2 2 CD cord-001249-awn9ayy6 194 102 ( ( -LRB- cord-001249-awn9ayy6 194 103 770Q771 770q771 CD cord-001249-awn9ayy6 194 104 aa aa NNP cord-001249-awn9ayy6 194 105 ) ) -RRB- cord-001249-awn9ayy6 194 106 and and CC cord-001249-awn9ayy6 194 107 TM TM NNP cord-001249-awn9ayy6 194 108 4 4 CD cord-001249-awn9ayy6 194 109 ( ( -LRB- cord-001249-awn9ayy6 194 110 927Q928 927q928 CD cord-001249-awn9ayy6 194 111 aa aa NNP cord-001249-awn9ayy6 194 112 ) ) -RRB- cord-001249-awn9ayy6 194 113 . . . cord-001249-awn9ayy6 195 1 Based base VBN cord-001249-awn9ayy6 195 2 on on IN cord-001249-awn9ayy6 195 3 the the DT cord-001249-awn9ayy6 195 4 NSDV NSDV NNP cord-001249-awn9ayy6 195 5 M M NNP cord-001249-awn9ayy6 195 6 topology topology NN cord-001249-awn9ayy6 195 7 prediction prediction NN cord-001249-awn9ayy6 195 8 , , , cord-001249-awn9ayy6 195 9 plasmids plasmid NNS cord-001249-awn9ayy6 195 10 encoding encode VBG cord-001249-awn9ayy6 195 11 PreGn pregn PRP cord-001249-awn9ayy6 195 12 , , , cord-001249-awn9ayy6 195 13 NS NS NNP cord-001249-awn9ayy6 195 14 M M NNP cord-001249-awn9ayy6 195 15 and and CC cord-001249-awn9ayy6 195 16 PreGc PreGc NNP cord-001249-awn9ayy6 195 17 ( ( -LRB- cord-001249-awn9ayy6 195 18 corresponding correspond VBG cord-001249-awn9ayy6 195 19 respectively respectively RB cord-001249-awn9ayy6 195 20 to to IN cord-001249-awn9ayy6 195 21 aa aa NNP cord-001249-awn9ayy6 195 22 1 1 NNP cord-001249-awn9ayy6 195 23 - - HYPH cord-001249-awn9ayy6 195 24 770 770 CD cord-001249-awn9ayy6 195 25 , , , cord-001249-awn9ayy6 195 26 750 750 CD cord-001249-awn9ayy6 195 27 - - HYPH cord-001249-awn9ayy6 195 28 927 927 CD cord-001249-awn9ayy6 195 29 and and CC cord-001249-awn9ayy6 195 30 907 907 CD cord-001249-awn9ayy6 195 31 - - HYPH cord-001249-awn9ayy6 195 32 1624 1624 CD cord-001249-awn9ayy6 195 33 of of IN cord-001249-awn9ayy6 195 34 the the DT cord-001249-awn9ayy6 195 35 NSDVi NSDVi NNS cord-001249-awn9ayy6 195 36 M m CD cord-001249-awn9ayy6 195 37 open open JJ cord-001249-awn9ayy6 195 38 reading reading NN cord-001249-awn9ayy6 195 39 frame frame NN cord-001249-awn9ayy6 195 40 ) ) -RRB- cord-001249-awn9ayy6 195 41 were be VBD cord-001249-awn9ayy6 195 42 constructed construct VBN cord-001249-awn9ayy6 195 43 in in IN cord-001249-awn9ayy6 195 44 which which WDT cord-001249-awn9ayy6 195 45 each each DT cord-001249-awn9ayy6 195 46 protein protein NN cord-001249-awn9ayy6 195 47 was be VBD cord-001249-awn9ayy6 195 48 in in IN cord-001249-awn9ayy6 195 49 frame frame NN cord-001249-awn9ayy6 195 50 with with IN cord-001249-awn9ayy6 195 51 a a DT cord-001249-awn9ayy6 195 52 carboxyterminal carboxyterminal JJ cord-001249-awn9ayy6 195 53 V5 V5 NNP cord-001249-awn9ayy6 195 54 epitope epitope NN cord-001249-awn9ayy6 195 55 tag tag NN cord-001249-awn9ayy6 195 56 . . . cord-001249-awn9ayy6 196 1 Expression expression NN cord-001249-awn9ayy6 196 2 of of IN cord-001249-awn9ayy6 196 3 these these DT cord-001249-awn9ayy6 196 4 constructs construct NNS cord-001249-awn9ayy6 196 5 was be VBD cord-001249-awn9ayy6 196 6 tested test VBN cord-001249-awn9ayy6 196 7 in in IN cord-001249-awn9ayy6 196 8 Vero Vero NNP cord-001249-awn9ayy6 196 9 cells cell NNS cord-001249-awn9ayy6 196 10 by by IN cord-001249-awn9ayy6 196 11 Western western JJ cord-001249-awn9ayy6 196 12 blotting blotting NN cord-001249-awn9ayy6 196 13 and and CC cord-001249-awn9ayy6 196 14 immunofluorescence immunofluorescence NN cord-001249-awn9ayy6 196 15 to to TO cord-001249-awn9ayy6 196 16 ensure ensure VB cord-001249-awn9ayy6 196 17 that that IN cord-001249-awn9ayy6 196 18 proteins protein NNS cord-001249-awn9ayy6 196 19 of of IN cord-001249-awn9ayy6 196 20 the the DT cord-001249-awn9ayy6 196 21 expected expect VBN cord-001249-awn9ayy6 196 22 size size NN cord-001249-awn9ayy6 196 23 and and CC cord-001249-awn9ayy6 196 24 localising localise VBG cord-001249-awn9ayy6 196 25 to to IN cord-001249-awn9ayy6 196 26 the the DT cord-001249-awn9ayy6 196 27 expected expect VBN cord-001249-awn9ayy6 196 28 compartments compartment NNS cord-001249-awn9ayy6 196 29 were be VBD cord-001249-awn9ayy6 196 30 expressed express VBN cord-001249-awn9ayy6 196 31 . . . cord-001249-awn9ayy6 197 1 The the DT cord-001249-awn9ayy6 197 2 plasmid plasmid NN cord-001249-awn9ayy6 197 3 - - VBD cord-001249-awn9ayy6 197 4 expressed express VBN cord-001249-awn9ayy6 197 5 PreGn PreGn NNP cord-001249-awn9ayy6 197 6 - - HYPH cord-001249-awn9ayy6 197 7 V5 V5 NNP cord-001249-awn9ayy6 197 8 was be VBD cord-001249-awn9ayy6 197 9 found find VBN cord-001249-awn9ayy6 197 10 in in IN cord-001249-awn9ayy6 197 11 the the DT cord-001249-awn9ayy6 197 12 ER ER NNP cord-001249-awn9ayy6 197 13 and and CC cord-001249-awn9ayy6 197 14 cisGolgi cisGolgi NNP cord-001249-awn9ayy6 197 15 , , , cord-001249-awn9ayy6 197 16 while while IN cord-001249-awn9ayy6 197 17 the the DT cord-001249-awn9ayy6 197 18 PreGc PreGc NNP cord-001249-awn9ayy6 197 19 - - HYPH cord-001249-awn9ayy6 197 20 V5 V5 NNP cord-001249-awn9ayy6 197 21 was be VBD cord-001249-awn9ayy6 197 22 found find VBN cord-001249-awn9ayy6 197 23 only only RB cord-001249-awn9ayy6 197 24 in in IN cord-001249-awn9ayy6 197 25 the the DT cord-001249-awn9ayy6 197 26 ER ER NNP cord-001249-awn9ayy6 197 27 , , , cord-001249-awn9ayy6 197 28 with with IN cord-001249-awn9ayy6 197 29 no no DT cord-001249-awn9ayy6 197 30 detectable detectable JJ cord-001249-awn9ayy6 197 31 colocalisation colocalisation NN cord-001249-awn9ayy6 197 32 with with IN cord-001249-awn9ayy6 197 33 the the DT cord-001249-awn9ayy6 197 34 cisGolgi cisgolgi JJ cord-001249-awn9ayy6 197 35 marker marker NN cord-001249-awn9ayy6 197 36 ( ( -LRB- cord-001249-awn9ayy6 197 37 Figure figure NN cord-001249-awn9ayy6 197 38 S5 S5 NNP cord-001249-awn9ayy6 197 39 a a NNP cord-001249-awn9ayy6 197 40 - - HYPH cord-001249-awn9ayy6 197 41 f f NN cord-001249-awn9ayy6 197 42 ) ) -RRB- cord-001249-awn9ayy6 197 43 . . . cord-001249-awn9ayy6 198 1 Some some DT cord-001249-awn9ayy6 198 2 of of IN cord-001249-awn9ayy6 198 3 the the DT cord-001249-awn9ayy6 198 4 plasmid plasmid NN cord-001249-awn9ayy6 198 5 - - HYPH cord-001249-awn9ayy6 198 6 expressed express VBN cord-001249-awn9ayy6 198 7 NS NS NNP cord-001249-awn9ayy6 198 8 M M NNP cord-001249-awn9ayy6 198 9 -V5 -v5 NN cord-001249-awn9ayy6 198 10 protein protein NN cord-001249-awn9ayy6 198 11 colocalised colocalise VBN cord-001249-awn9ayy6 198 12 with with IN cord-001249-awn9ayy6 198 13 the the DT cord-001249-awn9ayy6 198 14 cisGolgi cisgolgi JJ cord-001249-awn9ayy6 198 15 marker marker NN cord-001249-awn9ayy6 198 16 , , , cord-001249-awn9ayy6 198 17 however however RB cord-001249-awn9ayy6 198 18 NS NS NNP cord-001249-awn9ayy6 198 19 M M NNP cord-001249-awn9ayy6 198 20 -V5 -v5 NN cord-001249-awn9ayy6 198 21 also also RB cord-001249-awn9ayy6 198 22 appeared appear VBD cord-001249-awn9ayy6 198 23 in in IN cord-001249-awn9ayy6 198 24 other other JJ cord-001249-awn9ayy6 198 25 compartments compartment NNS cord-001249-awn9ayy6 198 26 of of IN cord-001249-awn9ayy6 198 27 the the DT cord-001249-awn9ayy6 198 28 secretory secretory JJ cord-001249-awn9ayy6 198 29 pathway pathway NN cord-001249-awn9ayy6 198 30 , , , cord-001249-awn9ayy6 198 31 one one CD cord-001249-awn9ayy6 198 32 of of IN cord-001249-awn9ayy6 198 33 which which WDT cord-001249-awn9ayy6 198 34 could could MD cord-001249-awn9ayy6 198 35 be be VB cord-001249-awn9ayy6 198 36 ERGIC ERGIC NNP cord-001249-awn9ayy6 199 1 ( ( -LRB- cord-001249-awn9ayy6 199 2 Figure figure NN cord-001249-awn9ayy6 199 3 S5 S5 NNP cord-001249-awn9ayy6 199 4 g g NNP cord-001249-awn9ayy6 199 5 - - : cord-001249-awn9ayy6 199 6 i i NNP cord-001249-awn9ayy6 199 7 ) ) -RRB- cord-001249-awn9ayy6 199 8 . . . cord-001249-awn9ayy6 200 1 Vero Vero NNP cord-001249-awn9ayy6 200 2 cells cell NNS cord-001249-awn9ayy6 200 3 were be VBD cord-001249-awn9ayy6 200 4 transfected transfecte VBN cord-001249-awn9ayy6 200 5 with with IN cord-001249-awn9ayy6 200 6 the the DT cord-001249-awn9ayy6 200 7 PreGn PreGn NNP cord-001249-awn9ayy6 200 8 - - HYPH cord-001249-awn9ayy6 200 9 V5 V5 NNP cord-001249-awn9ayy6 200 10 , , , cord-001249-awn9ayy6 200 11 PreGc pregc NN cord-001249-awn9ayy6 200 12 - - HYPH cord-001249-awn9ayy6 200 13 V5 V5 NNP cord-001249-awn9ayy6 200 14 and and CC cord-001249-awn9ayy6 200 15 NS NS NNP cord-001249-awn9ayy6 200 16 M M NNP cord-001249-awn9ayy6 200 17 -V5 -v5 NN cord-001249-awn9ayy6 200 18 expression expression NN cord-001249-awn9ayy6 200 19 constructs construct NNS cord-001249-awn9ayy6 200 20 and and CC cord-001249-awn9ayy6 200 21 their -PRON- PRP$ cord-001249-awn9ayy6 200 22 effect effect NN cord-001249-awn9ayy6 200 23 on on IN cord-001249-awn9ayy6 200 24 PDI PDI NNP cord-001249-awn9ayy6 200 25 distribution distribution NN cord-001249-awn9ayy6 200 26 in in IN cord-001249-awn9ayy6 200 27 transfected transfecte VBN cord-001249-awn9ayy6 200 28 cells cell NNS cord-001249-awn9ayy6 200 29 was be VBD cord-001249-awn9ayy6 200 30 investigated investigate VBN cord-001249-awn9ayy6 200 31 by by IN cord-001249-awn9ayy6 200 32 confocal confocal JJ cord-001249-awn9ayy6 200 33 microscopy microscopy NNS cord-001249-awn9ayy6 200 34 . . . cord-001249-awn9ayy6 201 1 Cells cell NNS cord-001249-awn9ayy6 201 2 which which WDT cord-001249-awn9ayy6 201 3 expressed express VBD cord-001249-awn9ayy6 201 4 PreGn pregn NN cord-001249-awn9ayy6 201 5 - - HYPH cord-001249-awn9ayy6 201 6 V5 V5 NNP cord-001249-awn9ayy6 201 7 lost lose VBN cord-001249-awn9ayy6 201 8 PDI PDI NNP cord-001249-awn9ayy6 201 9 from from IN cord-001249-awn9ayy6 201 10 the the DT cord-001249-awn9ayy6 201 11 ER er NN cord-001249-awn9ayy6 202 1 ( ( -LRB- cord-001249-awn9ayy6 202 2 Figure figure NN cord-001249-awn9ayy6 202 3 9 9 CD cord-001249-awn9ayy6 202 4 a a DT cord-001249-awn9ayy6 202 5 - - HYPH cord-001249-awn9ayy6 202 6 c c NN cord-001249-awn9ayy6 202 7 ) ) -RRB- cord-001249-awn9ayy6 202 8 , , , cord-001249-awn9ayy6 202 9 while while IN cord-001249-awn9ayy6 202 10 expression expression NN cord-001249-awn9ayy6 202 11 of of IN cord-001249-awn9ayy6 202 12 PreGc PreGc NNP cord-001249-awn9ayy6 202 13 - - HYPH cord-001249-awn9ayy6 202 14 V5 V5 NNP cord-001249-awn9ayy6 202 15 or or CC cord-001249-awn9ayy6 202 16 NS NS NNP cord-001249-awn9ayy6 202 17 M M NNP cord-001249-awn9ayy6 202 18 -V5 -v5 NN cord-001249-awn9ayy6 202 19 had have VBD cord-001249-awn9ayy6 202 20 no no DT cord-001249-awn9ayy6 202 21 apparent apparent JJ cord-001249-awn9ayy6 202 22 effect effect NN cord-001249-awn9ayy6 202 23 on on IN cord-001249-awn9ayy6 202 24 the the DT cord-001249-awn9ayy6 202 25 PDI PDI NNP cord-001249-awn9ayy6 202 26 distribution distribution NN cord-001249-awn9ayy6 202 27 ( ( -LRB- cord-001249-awn9ayy6 202 28 Figure figure NN cord-001249-awn9ayy6 202 29 9 9 CD cord-001249-awn9ayy6 202 30 d d XX cord-001249-awn9ayy6 202 31 - - : cord-001249-awn9ayy6 202 32 i i NNP cord-001249-awn9ayy6 202 33 ) ) -RRB- cord-001249-awn9ayy6 202 34 , , , cord-001249-awn9ayy6 202 35 suggesting suggest VBG cord-001249-awn9ayy6 202 36 that that IN cord-001249-awn9ayy6 202 37 it -PRON- PRP cord-001249-awn9ayy6 202 38 is be VBZ cord-001249-awn9ayy6 202 39 the the DT cord-001249-awn9ayy6 202 40 ( ( -LRB- cord-001249-awn9ayy6 202 41 Pre)Gn pre)gn CD cord-001249-awn9ayy6 202 42 but but CC cord-001249-awn9ayy6 202 43 not not RB cord-001249-awn9ayy6 202 44 ( ( -LRB- cord-001249-awn9ayy6 202 45 Pre)Gc Pre)Gc NNP cord-001249-awn9ayy6 202 46 or or CC cord-001249-awn9ayy6 202 47 NS NS NNP cord-001249-awn9ayy6 202 48 M M NNP cord-001249-awn9ayy6 202 49 which which WDT cord-001249-awn9ayy6 202 50 is be VBZ cord-001249-awn9ayy6 202 51 primarily primarily RB cord-001249-awn9ayy6 202 52 responsible responsible JJ cord-001249-awn9ayy6 202 53 for for IN cord-001249-awn9ayy6 202 54 the the DT cord-001249-awn9ayy6 202 55 translocation translocation NN cord-001249-awn9ayy6 202 56 of of IN cord-001249-awn9ayy6 202 57 PDI PDI NNP cord-001249-awn9ayy6 202 58 , , , cord-001249-awn9ayy6 202 59 and and CC cord-001249-awn9ayy6 202 60 possibly possibly RB cord-001249-awn9ayy6 202 61 ERp57 ERp57 NNS cord-001249-awn9ayy6 202 62 , , , cord-001249-awn9ayy6 202 63 to to IN cord-001249-awn9ayy6 202 64 the the DT cord-001249-awn9ayy6 202 65 cell cell NN cord-001249-awn9ayy6 202 66 surface surface NN cord-001249-awn9ayy6 202 67 . . . cord-001249-awn9ayy6 203 1 Although although IN cord-001249-awn9ayy6 203 2 in in IN cord-001249-awn9ayy6 203 3 some some DT cord-001249-awn9ayy6 203 4 cells cell NNS cord-001249-awn9ayy6 203 5 over over IN cord-001249-awn9ayy6 203 6 - - HYPH cord-001249-awn9ayy6 203 7 accumulation accumulation NN cord-001249-awn9ayy6 203 8 of of IN cord-001249-awn9ayy6 203 9 PreGn PreGn NNP cord-001249-awn9ayy6 203 10 - - HYPH cord-001249-awn9ayy6 203 11 V5 V5 NNP cord-001249-awn9ayy6 203 12 appeared appear VBD cord-001249-awn9ayy6 203 13 to to TO cord-001249-awn9ayy6 203 14 change change VB cord-001249-awn9ayy6 203 15 the the DT cord-001249-awn9ayy6 203 16 morphology morphology NN cord-001249-awn9ayy6 203 17 of of IN cord-001249-awn9ayy6 203 18 the the DT cord-001249-awn9ayy6 203 19 ER er NN cord-001249-awn9ayy6 203 20 , , , cord-001249-awn9ayy6 204 1 the the DT cord-001249-awn9ayy6 204 2 cells cell NNS cord-001249-awn9ayy6 204 3 which which WDT cord-001249-awn9ayy6 204 4 showed show VBD cord-001249-awn9ayy6 204 5 only only RB cord-001249-awn9ayy6 204 6 moderate moderate JJ cord-001249-awn9ayy6 204 7 or or CC cord-001249-awn9ayy6 204 8 low low JJ cord-001249-awn9ayy6 204 9 levels level NNS cord-001249-awn9ayy6 204 10 of of IN cord-001249-awn9ayy6 204 11 PreGn pregn JJ cord-001249-awn9ayy6 204 12 - - HYPH cord-001249-awn9ayy6 204 13 V5 V5 NNP cord-001249-awn9ayy6 204 14 in in IN cord-001249-awn9ayy6 204 15 the the DT cord-001249-awn9ayy6 204 16 ER ER NNP cord-001249-awn9ayy6 204 17 also also RB cord-001249-awn9ayy6 204 18 lost lose VBD cord-001249-awn9ayy6 204 19 PDI PDI NNP cord-001249-awn9ayy6 204 20 from from IN cord-001249-awn9ayy6 204 21 this this DT cord-001249-awn9ayy6 204 22 compartment compartment NN cord-001249-awn9ayy6 204 23 . . . cord-001249-awn9ayy6 205 1 In in IN cord-001249-awn9ayy6 205 2 cells cell NNS cord-001249-awn9ayy6 205 3 in in IN cord-001249-awn9ayy6 205 4 which which WDT cord-001249-awn9ayy6 205 5 PreGn pregn NN cord-001249-awn9ayy6 205 6 - - HYPH cord-001249-awn9ayy6 205 7 V5 V5 NNP cord-001249-awn9ayy6 205 8 was be VBD cord-001249-awn9ayy6 205 9 found find VBN cord-001249-awn9ayy6 205 10 only only RB cord-001249-awn9ayy6 205 11 in in IN cord-001249-awn9ayy6 205 12 the the DT cord-001249-awn9ayy6 205 13 Golgi Golgi NNP cord-001249-awn9ayy6 205 14 ( ( -LRB- cord-001249-awn9ayy6 205 15 i.e. i.e. FW cord-001249-awn9ayy6 205 16 cells cell NNS cord-001249-awn9ayy6 205 17 which which WDT cord-001249-awn9ayy6 205 18 expressed express VBD cord-001249-awn9ayy6 205 19 PreGn pregn PRP cord-001249-awn9ayy6 205 20 at at IN cord-001249-awn9ayy6 205 21 a a DT cord-001249-awn9ayy6 205 22 very very RB cord-001249-awn9ayy6 205 23 low low JJ cord-001249-awn9ayy6 205 24 level level NN cord-001249-awn9ayy6 205 25 ) ) -RRB- cord-001249-awn9ayy6 206 1 no no DT cord-001249-awn9ayy6 206 2 change change NN cord-001249-awn9ayy6 206 3 to to IN cord-001249-awn9ayy6 206 4 PDI PDI NNP cord-001249-awn9ayy6 206 5 was be VBD cord-001249-awn9ayy6 206 6 observed observe VBN cord-001249-awn9ayy6 206 7 , , , cord-001249-awn9ayy6 206 8 suggesting suggest VBG cord-001249-awn9ayy6 206 9 that that IN cord-001249-awn9ayy6 206 10 PreGn pregn PRP cord-001249-awn9ayy6 206 11 needs need VBZ cord-001249-awn9ayy6 206 12 to to TO cord-001249-awn9ayy6 206 13 accumulate accumulate VB cord-001249-awn9ayy6 206 14 in in IN cord-001249-awn9ayy6 206 15 the the DT cord-001249-awn9ayy6 206 16 ER ER NNP cord-001249-awn9ayy6 206 17 , , , cord-001249-awn9ayy6 206 18 even even RB cord-001249-awn9ayy6 206 19 at at IN cord-001249-awn9ayy6 206 20 low low JJ cord-001249-awn9ayy6 206 21 levels level NNS cord-001249-awn9ayy6 206 22 , , , cord-001249-awn9ayy6 206 23 in in IN cord-001249-awn9ayy6 206 24 order order NN cord-001249-awn9ayy6 206 25 to to TO cord-001249-awn9ayy6 206 26 affect affect VB cord-001249-awn9ayy6 206 27 the the DT cord-001249-awn9ayy6 206 28 distribution distribution NN cord-001249-awn9ayy6 206 29 of of IN cord-001249-awn9ayy6 206 30 PDI PDI NNP cord-001249-awn9ayy6 206 31 . . . cord-001249-awn9ayy6 207 1 PreGn PreGn NNP cord-001249-awn9ayy6 207 2 - - HYPH cord-001249-awn9ayy6 207 3 V5 V5 NNP cord-001249-awn9ayy6 207 4 had have VBD cord-001249-awn9ayy6 207 5 no no DT cord-001249-awn9ayy6 207 6 effect effect NN cord-001249-awn9ayy6 207 7 on on IN cord-001249-awn9ayy6 207 8 the the DT cord-001249-awn9ayy6 207 9 distribution distribution NN cord-001249-awn9ayy6 207 10 of of IN cord-001249-awn9ayy6 207 11 calnexin calnexin NNP cord-001249-awn9ayy6 207 12 ( ( -LRB- cord-001249-awn9ayy6 207 13 Figure figure NN cord-001249-awn9ayy6 207 14 9 9 CD cord-001249-awn9ayy6 207 15 j j NNP cord-001249-awn9ayy6 207 16 - - HYPH cord-001249-awn9ayy6 207 17 l l NNP cord-001249-awn9ayy6 207 18 ) ) -RRB- cord-001249-awn9ayy6 207 19 , , , cord-001249-awn9ayy6 207 20 indicating indicate VBG cord-001249-awn9ayy6 207 21 that that IN cord-001249-awn9ayy6 207 22 the the DT cord-001249-awn9ayy6 207 23 PreGn PreGn NNP cord-001249-awn9ayy6 207 24 - - HYPH cord-001249-awn9ayy6 207 25 V5 V5 NNP cord-001249-awn9ayy6 207 26 does do VBZ cord-001249-awn9ayy6 207 27 not not RB cord-001249-awn9ayy6 207 28 affect affect VB cord-001249-awn9ayy6 207 29 the the DT cord-001249-awn9ayy6 207 30 overall overall JJ cord-001249-awn9ayy6 207 31 ER ER NNP cord-001249-awn9ayy6 207 32 structure structure NN cord-001249-awn9ayy6 207 33 when when WRB cord-001249-awn9ayy6 207 34 expressed express VBN cord-001249-awn9ayy6 207 35 at at IN cord-001249-awn9ayy6 207 36 moderate moderate JJ cord-001249-awn9ayy6 207 37 levels level NNS cord-001249-awn9ayy6 207 38 , , , cord-001249-awn9ayy6 207 39 similarly similarly RB cord-001249-awn9ayy6 207 40 to to IN cord-001249-awn9ayy6 207 41 what what WP cord-001249-awn9ayy6 207 42 was be VBD cord-001249-awn9ayy6 207 43 observed observe VBN cord-001249-awn9ayy6 207 44 in in IN cord-001249-awn9ayy6 207 45 NSDV NSDV NNP cord-001249-awn9ayy6 207 46 - - HYPH cord-001249-awn9ayy6 207 47 infected infect VBN cord-001249-awn9ayy6 207 48 cells cell NNS cord-001249-awn9ayy6 207 49 . . . cord-001249-awn9ayy6 208 1 As as IN cord-001249-awn9ayy6 208 2 expected expect VBN cord-001249-awn9ayy6 208 3 , , , cord-001249-awn9ayy6 208 4 expression expression NN cord-001249-awn9ayy6 208 5 of of IN cord-001249-awn9ayy6 208 6 the the DT cord-001249-awn9ayy6 208 7 viral viral JJ cord-001249-awn9ayy6 208 8 N n CD cord-001249-awn9ayy6 208 9 protein protein NN cord-001249-awn9ayy6 208 10 or or CC cord-001249-awn9ayy6 208 11 the the DT cord-001249-awn9ayy6 208 12 N n CD cord-001249-awn9ayy6 208 13 - - HYPH cord-001249-awn9ayy6 208 14 terminal terminal JJ cord-001249-awn9ayy6 208 15 half half NN cord-001249-awn9ayy6 208 16 of of IN cord-001249-awn9ayy6 208 17 the the DT cord-001249-awn9ayy6 208 18 viral viral JJ cord-001249-awn9ayy6 208 19 L L NNP cord-001249-awn9ayy6 208 20 protein protein NN cord-001249-awn9ayy6 208 21 ( ( -LRB- cord-001249-awn9ayy6 208 22 La La NNP cord-001249-awn9ayy6 208 23 ) ) -RRB- cord-001249-awn9ayy6 208 24 [ [ -LRB- cord-001249-awn9ayy6 208 25 62 62 CD cord-001249-awn9ayy6 208 26 ] ] -RRB- cord-001249-awn9ayy6 208 27 had have VBD cord-001249-awn9ayy6 208 28 no no DT cord-001249-awn9ayy6 208 29 effect effect NN cord-001249-awn9ayy6 208 30 on on IN cord-001249-awn9ayy6 208 31 PDI PDI NNP cord-001249-awn9ayy6 208 32 distribution distribution NN cord-001249-awn9ayy6 208 33 ( ( -LRB- cord-001249-awn9ayy6 208 34 Figure figure NN cord-001249-awn9ayy6 208 35 S6 S6 NNP cord-001249-awn9ayy6 208 36 ) ) -RRB- cord-001249-awn9ayy6 208 37 . . . cord-001249-awn9ayy6 209 1 Taken take VBN cord-001249-awn9ayy6 209 2 together together RB cord-001249-awn9ayy6 209 3 , , , cord-001249-awn9ayy6 209 4 these these DT cord-001249-awn9ayy6 209 5 data datum NNS cord-001249-awn9ayy6 209 6 suggest suggest VBP cord-001249-awn9ayy6 209 7 that that IN cord-001249-awn9ayy6 209 8 NSDV NSDV NNP cord-001249-awn9ayy6 209 9 infection infection NN cord-001249-awn9ayy6 209 10 causes cause VBZ cord-001249-awn9ayy6 209 11 the the DT cord-001249-awn9ayy6 209 12 re re NN cord-001249-awn9ayy6 209 13 - - NN cord-001249-awn9ayy6 209 14 localisation localisation NN cord-001249-awn9ayy6 209 15 of of IN cord-001249-awn9ayy6 209 16 ER ER NNP cord-001249-awn9ayy6 209 17 soluble soluble JJ cord-001249-awn9ayy6 209 18 proteins protein NNS cord-001249-awn9ayy6 209 19 , , , cord-001249-awn9ayy6 209 20 notably notably RB cord-001249-awn9ayy6 209 21 PDI PDI NNP cord-001249-awn9ayy6 209 22 and and CC cord-001249-awn9ayy6 209 23 ERp57 ERp57 NNS cord-001249-awn9ayy6 209 24 , , , cord-001249-awn9ayy6 209 25 to to IN cord-001249-awn9ayy6 209 26 the the DT cord-001249-awn9ayy6 209 27 cell cell NN cord-001249-awn9ayy6 209 28 surface surface NN cord-001249-awn9ayy6 209 29 and and CC cord-001249-awn9ayy6 209 30 ultimately ultimately RB cord-001249-awn9ayy6 209 31 the the DT cord-001249-awn9ayy6 209 32 medium medium NN cord-001249-awn9ayy6 209 33 surrounding surround VBG cord-001249-awn9ayy6 209 34 the the DT cord-001249-awn9ayy6 209 35 infected infected JJ cord-001249-awn9ayy6 209 36 cell cell NN cord-001249-awn9ayy6 209 37 , , , cord-001249-awn9ayy6 209 38 and and CC cord-001249-awn9ayy6 209 39 this this DT cord-001249-awn9ayy6 209 40 re re NN cord-001249-awn9ayy6 209 41 - - NN cord-001249-awn9ayy6 209 42 localisation localisation NN cord-001249-awn9ayy6 209 43 appears appear VBZ cord-001249-awn9ayy6 209 44 to to TO cord-001249-awn9ayy6 209 45 be be VB cord-001249-awn9ayy6 209 46 due due JJ cord-001249-awn9ayy6 209 47 to to IN cord-001249-awn9ayy6 209 48 association association NN cord-001249-awn9ayy6 209 49 of of IN cord-001249-awn9ayy6 209 50 these these DT cord-001249-awn9ayy6 209 51 proteins protein NNS cord-001249-awn9ayy6 209 52 with with IN cord-001249-awn9ayy6 209 53 the the DT cord-001249-awn9ayy6 209 54 NSDV NSDV NNP cord-001249-awn9ayy6 209 55 ( ( -LRB- cord-001249-awn9ayy6 209 56 Pre)Gn Pre)Gn NNP cord-001249-awn9ayy6 209 57 glycoprotein glycoprotein NN cord-001249-awn9ayy6 209 58 . . . cord-001249-awn9ayy6 210 1 While while IN cord-001249-awn9ayy6 210 2 both both CC cord-001249-awn9ayy6 210 3 NSDV NSDV NNP cord-001249-awn9ayy6 210 4 and and CC cord-001249-awn9ayy6 210 5 CCHFV CCHFV NNP cord-001249-awn9ayy6 210 6 are be VBP cord-001249-awn9ayy6 210 7 nairoviruses nairoviruse NNS cord-001249-awn9ayy6 210 8 which which WDT cord-001249-awn9ayy6 210 9 cause cause VBP cord-001249-awn9ayy6 210 10 a a DT cord-001249-awn9ayy6 210 11 highly highly RB cord-001249-awn9ayy6 210 12 pathogenic pathogenic JJ cord-001249-awn9ayy6 210 13 haemorrhagic haemorrhagic JJ cord-001249-awn9ayy6 210 14 disease disease NN cord-001249-awn9ayy6 210 15 in in IN cord-001249-awn9ayy6 210 16 their -PRON- PRP$ cord-001249-awn9ayy6 210 17 natural natural JJ cord-001249-awn9ayy6 210 18 hosts host NNS cord-001249-awn9ayy6 210 19 , , , cord-001249-awn9ayy6 210 20 little little JJ cord-001249-awn9ayy6 210 21 is be VBZ cord-001249-awn9ayy6 210 22 known know VBN cord-001249-awn9ayy6 210 23 about about IN cord-001249-awn9ayy6 210 24 the the DT cord-001249-awn9ayy6 210 25 mechanism mechanism NN cord-001249-awn9ayy6 210 26 of of IN cord-001249-awn9ayy6 210 27 pathogenesis pathogenesis NN cord-001249-awn9ayy6 210 28 of of IN cord-001249-awn9ayy6 210 29 these these DT cord-001249-awn9ayy6 210 30 viruses virus NNS cord-001249-awn9ayy6 210 31 ; ; : cord-001249-awn9ayy6 210 32 even even RB cord-001249-awn9ayy6 210 33 the the DT cord-001249-awn9ayy6 210 34 primary primary JJ cord-001249-awn9ayy6 210 35 cellular cellular JJ cord-001249-awn9ayy6 210 36 targets target NNS cord-001249-awn9ayy6 210 37 are be VBP cord-001249-awn9ayy6 210 38 currently currently RB cord-001249-awn9ayy6 210 39 unknown unknown JJ cord-001249-awn9ayy6 210 40 . . . cord-001249-awn9ayy6 211 1 To to TO cord-001249-awn9ayy6 211 2 better better RB cord-001249-awn9ayy6 211 3 understand understand VB cord-001249-awn9ayy6 211 4 the the DT cord-001249-awn9ayy6 211 5 replication replication NN cord-001249-awn9ayy6 211 6 of of IN cord-001249-awn9ayy6 211 7 nairoviruses nairoviruse NNS cord-001249-awn9ayy6 211 8 we -PRON- PRP cord-001249-awn9ayy6 211 9 have have VBP cord-001249-awn9ayy6 211 10 studied study VBN cord-001249-awn9ayy6 211 11 NSDV NSDV NNP cord-001249-awn9ayy6 211 12 and and CC cord-001249-awn9ayy6 211 13 its -PRON- PRP$ cord-001249-awn9ayy6 211 14 effects effect NNS cord-001249-awn9ayy6 211 15 on on IN cord-001249-awn9ayy6 211 16 the the DT cord-001249-awn9ayy6 211 17 cellular cellular JJ cord-001249-awn9ayy6 211 18 compartments compartment NNS cord-001249-awn9ayy6 211 19 in in IN cord-001249-awn9ayy6 211 20 infected infected JJ cord-001249-awn9ayy6 211 21 cells cell NNS cord-001249-awn9ayy6 211 22 , , , cord-001249-awn9ayy6 211 23 particularly particularly RB cord-001249-awn9ayy6 211 24 concentrating concentrate VBG cord-001249-awn9ayy6 211 25 on on IN cord-001249-awn9ayy6 211 26 the the DT cord-001249-awn9ayy6 211 27 host host NN cord-001249-awn9ayy6 211 28 cell cell NN cord-001249-awn9ayy6 211 29 secretory secretory NN cord-001249-awn9ayy6 211 30 pathway pathway NN cord-001249-awn9ayy6 211 31 , , , cord-001249-awn9ayy6 211 32 which which WDT cord-001249-awn9ayy6 211 33 is be VBZ cord-001249-awn9ayy6 211 34 used use VBN cord-001249-awn9ayy6 211 35 by by IN cord-001249-awn9ayy6 211 36 nairoviruses nairoviruse NNS cord-001249-awn9ayy6 211 37 for for IN cord-001249-awn9ayy6 211 38 budding budding NN cord-001249-awn9ayy6 211 39 and and CC cord-001249-awn9ayy6 211 40 egress egress NN cord-001249-awn9ayy6 211 41 of of IN cord-001249-awn9ayy6 211 42 newly newly RB cord-001249-awn9ayy6 211 43 generated generate VBN cord-001249-awn9ayy6 211 44 virions virion NNS cord-001249-awn9ayy6 211 45 . . . cord-001249-awn9ayy6 212 1 Despite despite IN cord-001249-awn9ayy6 212 2 a a DT cord-001249-awn9ayy6 212 3 high high JJ cord-001249-awn9ayy6 212 4 throughput throughput NN cord-001249-awn9ayy6 212 5 of of IN cord-001249-awn9ayy6 212 6 viral viral JJ cord-001249-awn9ayy6 212 7 glycoproteins glycoprotein NNS cord-001249-awn9ayy6 212 8 processed process VBN cord-001249-awn9ayy6 212 9 through through IN cord-001249-awn9ayy6 212 10 the the DT cord-001249-awn9ayy6 212 11 secretory secretory JJ cord-001249-awn9ayy6 212 12 compartments compartment NNS cord-001249-awn9ayy6 212 13 , , , cord-001249-awn9ayy6 212 14 and and CC cord-001249-awn9ayy6 212 15 viral viral JJ cord-001249-awn9ayy6 212 16 budding bud VBG cord-001249-awn9ayy6 212 17 in in IN cord-001249-awn9ayy6 212 18 the the DT cord-001249-awn9ayy6 212 19 Golgi Golgi NNP cord-001249-awn9ayy6 212 20 , , , cord-001249-awn9ayy6 212 21 the the DT cord-001249-awn9ayy6 212 22 structure structure NN cord-001249-awn9ayy6 212 23 of of IN cord-001249-awn9ayy6 212 24 the the DT cord-001249-awn9ayy6 212 25 ER ER NNP cord-001249-awn9ayy6 212 26 and and CC cord-001249-awn9ayy6 212 27 post post JJ cord-001249-awn9ayy6 212 28 - - JJ cord-001249-awn9ayy6 212 29 ER er JJ cord-001249-awn9ayy6 212 30 compartments compartment NNS cord-001249-awn9ayy6 212 31 appeared appear VBD cord-001249-awn9ayy6 212 32 to to TO cord-001249-awn9ayy6 212 33 be be VB cord-001249-awn9ayy6 212 34 unaffected unaffected JJ cord-001249-awn9ayy6 212 35 in in IN cord-001249-awn9ayy6 212 36 NSDV NSDV NNP cord-001249-awn9ayy6 212 37 - - HYPH cord-001249-awn9ayy6 212 38 infected infect VBN cord-001249-awn9ayy6 212 39 cells cell NNS cord-001249-awn9ayy6 212 40 when when WRB cord-001249-awn9ayy6 212 41 investigated investigate VBN cord-001249-awn9ayy6 212 42 using use VBG cord-001249-awn9ayy6 212 43 immunofluorescence immunofluorescence NN cord-001249-awn9ayy6 212 44 . . . cord-001249-awn9ayy6 213 1 While while IN cord-001249-awn9ayy6 213 2 what what WP cord-001249-awn9ayy6 213 3 appear appear VBP cord-001249-awn9ayy6 213 4 to to TO cord-001249-awn9ayy6 213 5 be be VB cord-001249-awn9ayy6 213 6 swollen swollen JJ cord-001249-awn9ayy6 213 7 Golgi Golgi NNP cord-001249-awn9ayy6 213 8 cisternae cisternae NN cord-001249-awn9ayy6 213 9 and and CC cord-001249-awn9ayy6 213 10 vacuolisation vacuolisation NN cord-001249-awn9ayy6 213 11 of of IN cord-001249-awn9ayy6 213 12 the the DT cord-001249-awn9ayy6 213 13 cytoplasm cytoplasm NN cord-001249-awn9ayy6 213 14 has have VBZ cord-001249-awn9ayy6 213 15 been be VBN cord-001249-awn9ayy6 213 16 observed observe VBN cord-001249-awn9ayy6 213 17 by by IN cord-001249-awn9ayy6 213 18 electron electron NN cord-001249-awn9ayy6 213 19 microscopy microscopy NN cord-001249-awn9ayy6 213 20 in in IN cord-001249-awn9ayy6 213 21 NSDV NSDV NNP cord-001249-awn9ayy6 213 22 - - HYPH cord-001249-awn9ayy6 213 23 infected infect VBN cord-001249-awn9ayy6 213 24 cells cell NNS cord-001249-awn9ayy6 213 25 [ [ -LRB- cord-001249-awn9ayy6 213 26 35 35 CD cord-001249-awn9ayy6 213 27 ] ] -RRB- cord-001249-awn9ayy6 213 28 , , , cord-001249-awn9ayy6 213 29 it -PRON- PRP cord-001249-awn9ayy6 213 30 is be VBZ cord-001249-awn9ayy6 213 31 possible possible JJ cord-001249-awn9ayy6 213 32 that that IN cord-001249-awn9ayy6 213 33 changes change NNS cord-001249-awn9ayy6 213 34 to to IN cord-001249-awn9ayy6 213 35 the the DT cord-001249-awn9ayy6 213 36 Golgi Golgi NNP cord-001249-awn9ayy6 213 37 observed observe VBN cord-001249-awn9ayy6 213 38 by by IN cord-001249-awn9ayy6 213 39 electron electron NN cord-001249-awn9ayy6 213 40 microscopy microscopy NNS cord-001249-awn9ayy6 213 41 are be VBP cord-001249-awn9ayy6 213 42 not not RB cord-001249-awn9ayy6 213 43 extensive extensive JJ cord-001249-awn9ayy6 213 44 enough enough RB cord-001249-awn9ayy6 213 45 to to TO cord-001249-awn9ayy6 213 46 be be VB cord-001249-awn9ayy6 213 47 detected detect VBN cord-001249-awn9ayy6 213 48 by by IN cord-001249-awn9ayy6 213 49 confocal confocal JJ cord-001249-awn9ayy6 213 50 microscopy microscopy NNS cord-001249-awn9ayy6 213 51 , , , cord-001249-awn9ayy6 213 52 or or CC cord-001249-awn9ayy6 213 53 are be VBP cord-001249-awn9ayy6 213 54 not not RB cord-001249-awn9ayy6 213 55 reflected reflect VBN cord-001249-awn9ayy6 213 56 in in IN cord-001249-awn9ayy6 213 57 changes change NNS cord-001249-awn9ayy6 213 58 to to IN cord-001249-awn9ayy6 213 59 the the DT cord-001249-awn9ayy6 213 60 distribution distribution NN cord-001249-awn9ayy6 213 61 of of IN cord-001249-awn9ayy6 213 62 protein protein NN cord-001249-awn9ayy6 213 63 markers marker NNS cord-001249-awn9ayy6 213 64 of of IN cord-001249-awn9ayy6 213 65 those those DT cord-001249-awn9ayy6 213 66 compartments compartment NNS cord-001249-awn9ayy6 213 67 . . . cord-001249-awn9ayy6 214 1 Our -PRON- PRP$ cord-001249-awn9ayy6 214 2 findings finding NNS cord-001249-awn9ayy6 214 3 agree agree VBP cord-001249-awn9ayy6 214 4 with with IN cord-001249-awn9ayy6 214 5 the the DT cord-001249-awn9ayy6 214 6 absence absence NN cord-001249-awn9ayy6 214 7 of of IN cord-001249-awn9ayy6 214 8 changes change NNS cord-001249-awn9ayy6 214 9 to to IN cord-001249-awn9ayy6 214 10 the the DT cord-001249-awn9ayy6 214 11 Golgi Golgi NNP cord-001249-awn9ayy6 214 12 observed observe VBN cord-001249-awn9ayy6 214 13 in in IN cord-001249-awn9ayy6 214 14 cells cell NNS cord-001249-awn9ayy6 214 15 infected infect VBN cord-001249-awn9ayy6 214 16 with with IN cord-001249-awn9ayy6 214 17 CCHFV CCHFV NNP cord-001249-awn9ayy6 214 18 or or CC cord-001249-awn9ayy6 214 19 Hantaan Hantaan NNP cord-001249-awn9ayy6 214 20 virus virus NN cord-001249-awn9ayy6 214 21 ( ( -LRB- cord-001249-awn9ayy6 214 22 HTNV HTNV NNP cord-001249-awn9ayy6 214 23 ; ; : cord-001249-awn9ayy6 214 24 a a DT cord-001249-awn9ayy6 214 25 hantavirus hantavirus NN cord-001249-awn9ayy6 214 26 ) ) -RRB- cord-001249-awn9ayy6 214 27 [ [ -LRB- cord-001249-awn9ayy6 214 28 76 76 CD cord-001249-awn9ayy6 214 29 , , , cord-001249-awn9ayy6 214 30 77 77 CD cord-001249-awn9ayy6 214 31 ] ] -RRB- cord-001249-awn9ayy6 214 32 . . . cord-001249-awn9ayy6 215 1 In in IN cord-001249-awn9ayy6 215 2 contrast contrast NN cord-001249-awn9ayy6 215 3 , , , cord-001249-awn9ayy6 215 4 Bunyamwera Bunyamwera NNP cord-001249-awn9ayy6 215 5 virus virus NN cord-001249-awn9ayy6 215 6 ( ( -LRB- cord-001249-awn9ayy6 215 7 BUNV BUNV NNP cord-001249-awn9ayy6 215 8 ; ; : cord-001249-awn9ayy6 215 9 an an DT cord-001249-awn9ayy6 215 10 orthobunyavirus)-infected orthobunyavirus)-infected NNP cord-001249-awn9ayy6 215 11 cells cell NNS cord-001249-awn9ayy6 215 12 showed show VBD cord-001249-awn9ayy6 215 13 a a DT cord-001249-awn9ayy6 215 14 condensed condensed JJ cord-001249-awn9ayy6 215 15 phenotype phenotype NN cord-001249-awn9ayy6 215 16 of of IN cord-001249-awn9ayy6 215 17 the the DT cord-001249-awn9ayy6 215 18 cis cis NNP cord-001249-awn9ayy6 215 19 and and CC cord-001249-awn9ayy6 215 20 transGolgi transGolgi NNP cord-001249-awn9ayy6 215 21 [ [ -LRB- cord-001249-awn9ayy6 215 22 78 78 CD cord-001249-awn9ayy6 215 23 ] ] -RRB- cord-001249-awn9ayy6 215 24 [ [ -LRB- cord-001249-awn9ayy6 215 25 79 79 CD cord-001249-awn9ayy6 215 26 ] ] -RRB- cord-001249-awn9ayy6 215 27 [ [ -LRB- cord-001249-awn9ayy6 215 28 80 80 CD cord-001249-awn9ayy6 215 29 ] ] -RRB- cord-001249-awn9ayy6 215 30 , , , cord-001249-awn9ayy6 215 31 indicating indicate VBG cord-001249-awn9ayy6 215 32 that that IN cord-001249-awn9ayy6 215 33 different different JJ cord-001249-awn9ayy6 215 34 bunyaviruses bunyavirus NNS cord-001249-awn9ayy6 215 35 may may MD cord-001249-awn9ayy6 215 36 have have VB cord-001249-awn9ayy6 215 37 different different JJ cord-001249-awn9ayy6 215 38 effects effect NNS cord-001249-awn9ayy6 215 39 on on IN cord-001249-awn9ayy6 215 40 the the DT cord-001249-awn9ayy6 215 41 Golgi Golgi NNP cord-001249-awn9ayy6 215 42 . . . cord-001249-awn9ayy6 216 1 While while IN cord-001249-awn9ayy6 216 2 the the DT cord-001249-awn9ayy6 216 3 ERGIC ERGIC NNP cord-001249-awn9ayy6 216 4 has have VBZ cord-001249-awn9ayy6 216 5 been be VBN cord-001249-awn9ayy6 216 6 proposed propose VBN cord-001249-awn9ayy6 216 7 as as IN cord-001249-awn9ayy6 216 8 the the DT cord-001249-awn9ayy6 216 9 first first JJ cord-001249-awn9ayy6 216 10 site site NN cord-001249-awn9ayy6 216 11 of of IN cord-001249-awn9ayy6 216 12 budding bud VBG cord-001249-awn9ayy6 216 13 for for IN cord-001249-awn9ayy6 216 14 phleboviruses phleboviruse NNS cord-001249-awn9ayy6 216 15 , , , cord-001249-awn9ayy6 216 16 orthobunyaviruses orthobunyaviruse NNS cord-001249-awn9ayy6 216 17 and and CC cord-001249-awn9ayy6 216 18 hantaviruses hantaviruses NNPS cord-001249-awn9ayy6 216 19 [ [ -LRB- cord-001249-awn9ayy6 216 20 76 76 CD cord-001249-awn9ayy6 216 21 , , , cord-001249-awn9ayy6 216 22 [ [ -LRB- cord-001249-awn9ayy6 216 23 79 79 CD cord-001249-awn9ayy6 216 24 ] ] -RRB- cord-001249-awn9ayy6 216 25 [ [ -LRB- cord-001249-awn9ayy6 216 26 80 80 CD cord-001249-awn9ayy6 216 27 ] ] -RRB- cord-001249-awn9ayy6 216 28 [ [ -LRB- cord-001249-awn9ayy6 216 29 81 81 CD cord-001249-awn9ayy6 216 30 ] ] -RRB- cord-001249-awn9ayy6 216 31 [ [ -LRB- cord-001249-awn9ayy6 216 32 82 82 CD cord-001249-awn9ayy6 216 33 ] ] -RRB- cord-001249-awn9ayy6 216 34 [ [ -LRB- cord-001249-awn9ayy6 216 35 83 83 CD cord-001249-awn9ayy6 216 36 ] ] -RRB- cord-001249-awn9ayy6 216 37 , , , cord-001249-awn9ayy6 216 38 no no DT cord-001249-awn9ayy6 216 39 specific specific JJ cord-001249-awn9ayy6 216 40 accumulation accumulation NN cord-001249-awn9ayy6 216 41 of of IN cord-001249-awn9ayy6 216 42 the the DT cord-001249-awn9ayy6 216 43 NSDV NSDV NNP cord-001249-awn9ayy6 216 44 N n CD cord-001249-awn9ayy6 216 45 colocalising colocalising NN cord-001249-awn9ayy6 216 46 with with IN cord-001249-awn9ayy6 216 47 the the DT cord-001249-awn9ayy6 216 48 ERGIC ERGIC NNP cord-001249-awn9ayy6 216 49 marker marker NN cord-001249-awn9ayy6 216 50 was be VBD cord-001249-awn9ayy6 216 51 observed observe VBN cord-001249-awn9ayy6 216 52 in in IN cord-001249-awn9ayy6 216 53 our -PRON- PRP$ cord-001249-awn9ayy6 216 54 studies study NNS cord-001249-awn9ayy6 216 55 . . . cord-001249-awn9ayy6 217 1 Although although IN cord-001249-awn9ayy6 217 2 some some DT cord-001249-awn9ayy6 217 3 overlapping overlapping NN cord-001249-awn9ayy6 217 4 of of IN cord-001249-awn9ayy6 217 5 N n CD cord-001249-awn9ayy6 217 6 and and CC cord-001249-awn9ayy6 217 7 ERGIC53 ergic53 NN cord-001249-awn9ayy6 217 8 signal signal NN cord-001249-awn9ayy6 217 9 was be VBD cord-001249-awn9ayy6 217 10 noticed notice VBN cord-001249-awn9ayy6 217 11 in in IN cord-001249-awn9ayy6 217 12 some some DT cord-001249-awn9ayy6 217 13 of of IN cord-001249-awn9ayy6 217 14 the the DT cord-001249-awn9ayy6 217 15 infected infected JJ cord-001249-awn9ayy6 217 16 cells cell NNS cord-001249-awn9ayy6 217 17 , , , cord-001249-awn9ayy6 217 18 such such JJ cord-001249-awn9ayy6 217 19 apparent apparent JJ cord-001249-awn9ayy6 217 20 colocalisation colocalisation NN cord-001249-awn9ayy6 217 21 appeared appear VBD cord-001249-awn9ayy6 217 22 to to TO cord-001249-awn9ayy6 217 23 be be VB cord-001249-awn9ayy6 217 24 due due JJ cord-001249-awn9ayy6 217 25 to to IN cord-001249-awn9ayy6 217 26 the the DT cord-001249-awn9ayy6 217 27 accumulation accumulation NN cord-001249-awn9ayy6 217 28 of of IN cord-001249-awn9ayy6 217 29 very very RB cord-001249-awn9ayy6 217 30 high high JJ cord-001249-awn9ayy6 217 31 levels level NNS cord-001249-awn9ayy6 217 32 of of IN cord-001249-awn9ayy6 217 33 N n CD cord-001249-awn9ayy6 217 34 protein protein NN cord-001249-awn9ayy6 217 35 in in IN cord-001249-awn9ayy6 217 36 those those DT cord-001249-awn9ayy6 217 37 cells cell NNS cord-001249-awn9ayy6 217 38 . . . cord-001249-awn9ayy6 218 1 While while IN cord-001249-awn9ayy6 218 2 the the DT cord-001249-awn9ayy6 218 3 structure structure NN cord-001249-awn9ayy6 218 4 of of IN cord-001249-awn9ayy6 218 5 the the DT cord-001249-awn9ayy6 218 6 ER ER NNP cord-001249-awn9ayy6 218 7 remains remain VBZ cord-001249-awn9ayy6 218 8 unchanged unchanged JJ cord-001249-awn9ayy6 218 9 , , , cord-001249-awn9ayy6 218 10 NSDV NSDV NNP cord-001249-awn9ayy6 218 11 infection infection NN cord-001249-awn9ayy6 218 12 led lead VBD cord-001249-awn9ayy6 218 13 to to IN cord-001249-awn9ayy6 218 14 the the DT cord-001249-awn9ayy6 218 15 redistribution redistribution NN cord-001249-awn9ayy6 218 16 of of IN cord-001249-awn9ayy6 218 17 the the DT cord-001249-awn9ayy6 218 18 soluble soluble JJ cord-001249-awn9ayy6 218 19 oxidoreductases oxidoreductase NNS cord-001249-awn9ayy6 218 20 PDI PDI NNP cord-001249-awn9ayy6 218 21 and and CC cord-001249-awn9ayy6 218 22 ERp57 ERp57 NNS cord-001249-awn9ayy6 218 23 from from IN cord-001249-awn9ayy6 218 24 the the DT cord-001249-awn9ayy6 218 25 ER ER NNP cord-001249-awn9ayy6 218 26 to to IN cord-001249-awn9ayy6 218 27 the the DT cord-001249-awn9ayy6 218 28 cell cell NN cord-001249-awn9ayy6 218 29 surface surface NN cord-001249-awn9ayy6 218 30 , , , cord-001249-awn9ayy6 218 31 and and CC cord-001249-awn9ayy6 218 32 ultimately ultimately RB cord-001249-awn9ayy6 218 33 to to IN cord-001249-awn9ayy6 218 34 the the DT cord-001249-awn9ayy6 218 35 extracellular extracellular JJ cord-001249-awn9ayy6 218 36 space space NN cord-001249-awn9ayy6 218 37 ( ( -LRB- cord-001249-awn9ayy6 218 38 i.e. i.e. FW cord-001249-awn9ayy6 218 39 cell cell NN cord-001249-awn9ayy6 218 40 medium medium NN cord-001249-awn9ayy6 218 41 ) ) -RRB- cord-001249-awn9ayy6 218 42 . . . cord-001249-awn9ayy6 219 1 Redistribution redistribution NN cord-001249-awn9ayy6 219 2 of of IN cord-001249-awn9ayy6 219 3 PDI PDI NNP cord-001249-awn9ayy6 219 4 has have VBZ cord-001249-awn9ayy6 219 5 been be VBN cord-001249-awn9ayy6 219 6 observed observe VBN cord-001249-awn9ayy6 219 7 also also RB cord-001249-awn9ayy6 219 8 during during IN cord-001249-awn9ayy6 219 9 replication replication NN cord-001249-awn9ayy6 219 10 of of IN cord-001249-awn9ayy6 219 11 other other JJ cord-001249-awn9ayy6 219 12 viruses virus NNS cord-001249-awn9ayy6 219 13 . . . cord-001249-awn9ayy6 220 1 For for IN cord-001249-awn9ayy6 220 2 instance instance NN cord-001249-awn9ayy6 220 3 , , , cord-001249-awn9ayy6 220 4 PDI PDI NNP cord-001249-awn9ayy6 220 5 was be VBD cord-001249-awn9ayy6 220 6 redistributed redistribute VBN cord-001249-awn9ayy6 220 7 from from IN cord-001249-awn9ayy6 220 8 the the DT cord-001249-awn9ayy6 220 9 peripheral peripheral JJ cord-001249-awn9ayy6 220 10 ER er NN cord-001249-awn9ayy6 220 11 to to TO cord-001249-awn9ayy6 220 12 Figure figure VB cord-001249-awn9ayy6 220 13 6 6 CD cord-001249-awn9ayy6 220 14 . . . cord-001249-awn9ayy6 221 1 Time time NN cord-001249-awn9ayy6 221 2 course course NN cord-001249-awn9ayy6 221 3 of of IN cord-001249-awn9ayy6 221 4 changes change NNS cord-001249-awn9ayy6 221 5 to to IN cord-001249-awn9ayy6 221 6 PDI PDI NNP cord-001249-awn9ayy6 221 7 in in IN cord-001249-awn9ayy6 221 8 NSDV NSDV NNP cord-001249-awn9ayy6 221 9 - - HYPH cord-001249-awn9ayy6 221 10 infected infect VBN cord-001249-awn9ayy6 221 11 cells cell NNS cord-001249-awn9ayy6 221 12 . . . cord-001249-awn9ayy6 222 1 Vero Vero NNP cord-001249-awn9ayy6 222 2 cells cell NNS cord-001249-awn9ayy6 222 3 were be VBD cord-001249-awn9ayy6 222 4 infected infect VBN cord-001249-awn9ayy6 222 5 with with IN cord-001249-awn9ayy6 222 6 the the DT cord-001249-awn9ayy6 222 7 NSDVi NSDVi NNS cord-001249-awn9ayy6 222 8 isolate isolate VBP cord-001249-awn9ayy6 222 9 at at IN cord-001249-awn9ayy6 222 10 a a DT cord-001249-awn9ayy6 222 11 MOI MOI NNP cord-001249-awn9ayy6 222 12 of of IN cord-001249-awn9ayy6 222 13 6 6 CD cord-001249-awn9ayy6 222 14 TCID TCID NNP cord-001249-awn9ayy6 222 15 50 50 CD cord-001249-awn9ayy6 222 16 and and CC cord-001249-awn9ayy6 222 17 fixed fix VBN cord-001249-awn9ayy6 222 18 at at IN cord-001249-awn9ayy6 222 19 8 8 CD cord-001249-awn9ayy6 222 20 , , , cord-001249-awn9ayy6 222 21 12 12 CD cord-001249-awn9ayy6 222 22 and and CC cord-001249-awn9ayy6 222 23 16 16 CD cord-001249-awn9ayy6 222 24 hpi hpi NNP cord-001249-awn9ayy6 222 25 . . . cord-001249-awn9ayy6 223 1 For for IN cord-001249-awn9ayy6 223 2 internal internal JJ cord-001249-awn9ayy6 223 3 staining staining NN cord-001249-awn9ayy6 223 4 ( ( -LRB- cord-001249-awn9ayy6 223 5 a a FW cord-001249-awn9ayy6 223 6 - - : cord-001249-awn9ayy6 223 7 i i NN cord-001249-awn9ayy6 223 8 ) ) -RRB- cord-001249-awn9ayy6 223 9 cells cell NNS cord-001249-awn9ayy6 223 10 were be VBD cord-001249-awn9ayy6 223 11 fixed fix VBN cord-001249-awn9ayy6 223 12 using use VBG cord-001249-awn9ayy6 223 13 3 3 CD cord-001249-awn9ayy6 223 14 % % NN cord-001249-awn9ayy6 224 1 PFA PFA NNP cord-001249-awn9ayy6 224 2 followed follow VBD cord-001249-awn9ayy6 224 3 by by IN cord-001249-awn9ayy6 224 4 ice ice NN cord-001249-awn9ayy6 224 5 cold cold JJ cord-001249-awn9ayy6 224 6 methanol methanol NN cord-001249-awn9ayy6 224 7 . . . cord-001249-awn9ayy6 225 1 Cells cell NNS cord-001249-awn9ayy6 225 2 were be VBD cord-001249-awn9ayy6 225 3 co co JJ cord-001249-awn9ayy6 225 4 - - JJ cord-001249-awn9ayy6 225 5 stained stain VBN cord-001249-awn9ayy6 225 6 with with IN cord-001249-awn9ayy6 225 7 mouse mouse NN cord-001249-awn9ayy6 225 8 anti anti JJ cord-001249-awn9ayy6 225 9 - - NNP cord-001249-awn9ayy6 225 10 PDI pdi JJ cord-001249-awn9ayy6 225 11 ( ( -LRB- cord-001249-awn9ayy6 225 12 clone clone NN cord-001249-awn9ayy6 225 13 1D3 1d3 CD cord-001249-awn9ayy6 225 14 ) ) -RRB- cord-001249-awn9ayy6 225 15 antibody antibody NN cord-001249-awn9ayy6 225 16 and and CC cord-001249-awn9ayy6 225 17 rabbit rabbit NN cord-001249-awn9ayy6 225 18 antiserum antiserum NN cord-001249-awn9ayy6 225 19 against against IN cord-001249-awn9ayy6 225 20 the the DT cord-001249-awn9ayy6 225 21 viral viral JJ cord-001249-awn9ayy6 225 22 N n CD cord-001249-awn9ayy6 225 23 protein protein NN cord-001249-awn9ayy6 225 24 . . . cord-001249-awn9ayy6 226 1 For for IN cord-001249-awn9ayy6 226 2 surface surface NN cord-001249-awn9ayy6 226 3 PDI PDI NNP cord-001249-awn9ayy6 226 4 staining staining NN cord-001249-awn9ayy6 226 5 ( ( -LRB- cord-001249-awn9ayy6 226 6 j j NNP cord-001249-awn9ayy6 226 7 - - HYPH cord-001249-awn9ayy6 226 8 l l NNP cord-001249-awn9ayy6 226 9 ) ) -RRB- cord-001249-awn9ayy6 226 10 , , , cord-001249-awn9ayy6 226 11 cells cell NNS cord-001249-awn9ayy6 226 12 were be VBD cord-001249-awn9ayy6 226 13 fixed fix VBN cord-001249-awn9ayy6 226 14 with with IN cord-001249-awn9ayy6 226 15 3 3 CD cord-001249-awn9ayy6 226 16 % % NN cord-001249-awn9ayy6 227 1 PFA PFA NNP cord-001249-awn9ayy6 227 2 followed follow VBD cord-001249-awn9ayy6 227 3 by by IN cord-001249-awn9ayy6 227 4 staining stain VBG cord-001249-awn9ayy6 227 5 with with IN cord-001249-awn9ayy6 227 6 mouse mouse NN cord-001249-awn9ayy6 227 7 anti anti JJ cord-001249-awn9ayy6 227 8 - - NNP cord-001249-awn9ayy6 227 9 PDI pdi JJ cord-001249-awn9ayy6 227 10 ( ( -LRB- cord-001249-awn9ayy6 227 11 clone clone NN cord-001249-awn9ayy6 227 12 1D3 1d3 CD cord-001249-awn9ayy6 227 13 ) ) -RRB- cord-001249-awn9ayy6 227 14 antibody antibody NN cord-001249-awn9ayy6 227 15 . . . cord-001249-awn9ayy6 228 1 Then then RB cord-001249-awn9ayy6 228 2 cells cell NNS cord-001249-awn9ayy6 228 3 were be VBD cord-001249-awn9ayy6 228 4 again again RB cord-001249-awn9ayy6 228 5 fixed fix VBN cord-001249-awn9ayy6 228 6 with with IN cord-001249-awn9ayy6 228 7 3 3 CD cord-001249-awn9ayy6 228 8 % % NN cord-001249-awn9ayy6 228 9 PFA PFA NNP cord-001249-awn9ayy6 228 10 , , , cord-001249-awn9ayy6 228 11 opened open VBD cord-001249-awn9ayy6 228 12 with with IN cord-001249-awn9ayy6 228 13 ice ice NN cord-001249-awn9ayy6 228 14 - - HYPH cord-001249-awn9ayy6 228 15 cold cold JJ cord-001249-awn9ayy6 228 16 methanol methanol NN cord-001249-awn9ayy6 228 17 , , , cord-001249-awn9ayy6 228 18 and and CC cord-001249-awn9ayy6 228 19 virus virus NN cord-001249-awn9ayy6 228 20 was be VBD cord-001249-awn9ayy6 228 21 detected detect VBN cord-001249-awn9ayy6 228 22 by by IN cord-001249-awn9ayy6 228 23 rabbit rabbit NN cord-001249-awn9ayy6 228 24 antiserum antiserum NN cord-001249-awn9ayy6 228 25 against against IN cord-001249-awn9ayy6 228 26 the the DT cord-001249-awn9ayy6 228 27 N N NNP cord-001249-awn9ayy6 228 28 protein protein NN cord-001249-awn9ayy6 228 29 . . . cord-001249-awn9ayy6 229 1 Proteins protein NNS cord-001249-awn9ayy6 229 2 were be VBD cord-001249-awn9ayy6 229 3 visualised visualise VBN cord-001249-awn9ayy6 229 4 with with IN cord-001249-awn9ayy6 229 5 Alexa Alexa NNP cord-001249-awn9ayy6 229 6 Fluor Fluor NNP cord-001249-awn9ayy6 229 7 488 488 CD cord-001249-awn9ayy6 229 8 goat goat NN cord-001249-awn9ayy6 229 9 anti anti JJ cord-001249-awn9ayy6 229 10 - - JJ cord-001249-awn9ayy6 229 11 mouse mouse JJ cord-001249-awn9ayy6 229 12 IgG IgG NNP cord-001249-awn9ayy6 229 13 ( ( -LRB- cord-001249-awn9ayy6 229 14 green green NNP cord-001249-awn9ayy6 229 15 ) ) -RRB- cord-001249-awn9ayy6 229 16 and and CC cord-001249-awn9ayy6 229 17 Alexa Alexa NNP cord-001249-awn9ayy6 229 18 Fluor Fluor NNP cord-001249-awn9ayy6 229 19 568 568 CD cord-001249-awn9ayy6 229 20 goat goat NN cord-001249-awn9ayy6 229 21 anti anti JJ cord-001249-awn9ayy6 229 22 - - JJ cord-001249-awn9ayy6 229 23 rabbit rabbit JJ cord-001249-awn9ayy6 229 24 IgG igg NN cord-001249-awn9ayy6 229 25 ( ( -LRB- cord-001249-awn9ayy6 229 26 red red NNP cord-001249-awn9ayy6 229 27 ) ) -RRB- cord-001249-awn9ayy6 229 28 . . . cord-001249-awn9ayy6 230 1 Nuclei nucleus NNS cord-001249-awn9ayy6 230 2 were be VBD cord-001249-awn9ayy6 230 3 counterstained counterstaine VBN cord-001249-awn9ayy6 230 4 using use VBG cord-001249-awn9ayy6 230 5 DAPI DAPI NNP cord-001249-awn9ayy6 230 6 ( ( -LRB- cord-001249-awn9ayy6 230 7 blue blue JJ cord-001249-awn9ayy6 230 8 ) ) -RRB- cord-001249-awn9ayy6 230 9 . . . cord-001249-awn9ayy6 231 1 Bars bar NNS cord-001249-awn9ayy6 231 2 correspond correspond VBP cord-001249-awn9ayy6 231 3 to to IN cord-001249-awn9ayy6 231 4 16 16 CD cord-001249-awn9ayy6 231 5 mm mm NN cord-001249-awn9ayy6 231 6 . . . cord-001249-awn9ayy6 232 1 doi:10.1371 doi:10.1371 NNP cord-001249-awn9ayy6 233 1 /journal.pone.0094656.g006 /journal.pone.0094656.g006 NFP cord-001249-awn9ayy6 233 2 perinuclear perinuclear NNP cord-001249-awn9ayy6 233 3 membranous membranous JJ cord-001249-awn9ayy6 233 4 viral viral JJ cord-001249-awn9ayy6 233 5 factories factory NNS cord-001249-awn9ayy6 233 6 induced induce VBN cord-001249-awn9ayy6 233 7 by by IN cord-001249-awn9ayy6 233 8 Kunjin Kunjin NNP cord-001249-awn9ayy6 233 9 virus virus NN cord-001249-awn9ayy6 233 10 ( ( -LRB- cord-001249-awn9ayy6 233 11 KUNV KUNV NNPS cord-001249-awn9ayy6 233 12 ; ; : cord-001249-awn9ayy6 234 1 genus genus NNP cord-001249-awn9ayy6 234 2 Flavivirus Flavivirus NNP cord-001249-awn9ayy6 234 3 ) ) -RRB- cord-001249-awn9ayy6 234 4 [ [ -LRB- cord-001249-awn9ayy6 234 5 84 84 CD cord-001249-awn9ayy6 234 6 ] ] -RRB- cord-001249-awn9ayy6 234 7 . . . cord-001249-awn9ayy6 235 1 It -PRON- PRP cord-001249-awn9ayy6 235 2 is be VBZ cord-001249-awn9ayy6 235 3 currently currently RB cord-001249-awn9ayy6 235 4 unknown unknown JJ cord-001249-awn9ayy6 235 5 whether whether IN cord-001249-awn9ayy6 235 6 KUNV KUNV NNPS cord-001249-awn9ayy6 235 7 selectively selectively RB cord-001249-awn9ayy6 235 8 redistributes redistribute VBZ cord-001249-awn9ayy6 235 9 PDI PDI NNP cord-001249-awn9ayy6 235 10 or or CC cord-001249-awn9ayy6 235 11 also also RB cord-001249-awn9ayy6 235 12 affects affect VBZ cord-001249-awn9ayy6 235 13 other other JJ cord-001249-awn9ayy6 235 14 ER ER NNP cord-001249-awn9ayy6 235 15 proteins protein NNS cord-001249-awn9ayy6 235 16 ; ; : cord-001249-awn9ayy6 235 17 since since IN cord-001249-awn9ayy6 235 18 the the DT cord-001249-awn9ayy6 235 19 KUNV KUNV NNS cord-001249-awn9ayy6 235 20 glycoproteins glycoprotein NNS cord-001249-awn9ayy6 235 21 E e NN cord-001249-awn9ayy6 235 22 and and CC cord-001249-awn9ayy6 235 23 M M NNP cord-001249-awn9ayy6 235 24 mature mature VBP cord-001249-awn9ayy6 235 25 in in IN cord-001249-awn9ayy6 235 26 the the DT cord-001249-awn9ayy6 235 27 ER ER NNP cord-001249-awn9ayy6 235 28 [ [ -LRB- cord-001249-awn9ayy6 235 29 85 85 CD cord-001249-awn9ayy6 235 30 ] ] -RRB- cord-001249-awn9ayy6 235 31 and and CC cord-001249-awn9ayy6 235 32 KUNV KUNV NNP cord-001249-awn9ayy6 235 33 generates generate VBZ cord-001249-awn9ayy6 235 34 its -PRON- PRP$ cord-001249-awn9ayy6 235 35 viral viral JJ cord-001249-awn9ayy6 235 36 factories factory NNS cord-001249-awn9ayy6 235 37 by by IN cord-001249-awn9ayy6 235 38 remodelling remodelle VBG cord-001249-awn9ayy6 235 39 both both CC cord-001249-awn9ayy6 235 40 the the DT cord-001249-awn9ayy6 235 41 ER ER NNP cord-001249-awn9ayy6 235 42 and and CC cord-001249-awn9ayy6 235 43 transGolgi transgolgi RB cord-001249-awn9ayy6 235 44 [ [ -LRB- cord-001249-awn9ayy6 235 45 84 84 CD cord-001249-awn9ayy6 235 46 ] ] -RRB- cord-001249-awn9ayy6 235 47 , , , cord-001249-awn9ayy6 235 48 it -PRON- PRP cord-001249-awn9ayy6 235 49 is be VBZ cord-001249-awn9ayy6 235 50 possible possible JJ cord-001249-awn9ayy6 235 51 that that IN cord-001249-awn9ayy6 235 52 in in IN cord-001249-awn9ayy6 235 53 KUNVinfected kunvinfecte VBN cord-001249-awn9ayy6 235 54 cells cell NNS cord-001249-awn9ayy6 235 55 the the DT cord-001249-awn9ayy6 235 56 normal normal JJ cord-001249-awn9ayy6 235 57 retrieval retrieval NN cord-001249-awn9ayy6 235 58 system system NN cord-001249-awn9ayy6 235 59 of of IN cord-001249-awn9ayy6 235 60 ER ER NNP cord-001249-awn9ayy6 235 61 proteins protein NNS cord-001249-awn9ayy6 235 62 may may MD cord-001249-awn9ayy6 235 63 not not RB cord-001249-awn9ayy6 235 64 work work VB cord-001249-awn9ayy6 235 65 . . . cord-001249-awn9ayy6 236 1 Interestingly interestingly RB cord-001249-awn9ayy6 236 2 , , , cord-001249-awn9ayy6 236 3 despite despite IN cord-001249-awn9ayy6 236 4 changes change NNS cord-001249-awn9ayy6 236 5 to to IN cord-001249-awn9ayy6 236 6 the the DT cord-001249-awn9ayy6 236 7 ER ER NNP cord-001249-awn9ayy6 236 8 , , , cord-001249-awn9ayy6 236 9 no no DT cord-001249-awn9ayy6 236 10 major major JJ cord-001249-awn9ayy6 236 11 effect effect NN cord-001249-awn9ayy6 236 12 on on IN cord-001249-awn9ayy6 236 13 the the DT cord-001249-awn9ayy6 236 14 Golgi Golgi NNP cord-001249-awn9ayy6 236 15 structure structure NN cord-001249-awn9ayy6 236 16 was be VBD cord-001249-awn9ayy6 236 17 found find VBN cord-001249-awn9ayy6 236 18 by by IN cord-001249-awn9ayy6 236 19 immunofluorescence immunofluorescence NN cord-001249-awn9ayy6 236 20 in in IN cord-001249-awn9ayy6 236 21 KUNVinfected kunvinfected JJ cord-001249-awn9ayy6 236 22 cells cell NNS cord-001249-awn9ayy6 236 23 [ [ -LRB- cord-001249-awn9ayy6 236 24 84 84 CD cord-001249-awn9ayy6 236 25 ] ] -RRB- cord-001249-awn9ayy6 236 26 . . . cord-001249-awn9ayy6 237 1 Another another DT cord-001249-awn9ayy6 237 2 flavivirus flavivirus NN cord-001249-awn9ayy6 237 3 , , , cord-001249-awn9ayy6 237 4 Dengue Dengue NNP cord-001249-awn9ayy6 237 5 virus virus NN cord-001249-awn9ayy6 237 6 ( ( -LRB- cord-001249-awn9ayy6 237 7 DENV DENV NNP cord-001249-awn9ayy6 237 8 ) ) -RRB- cord-001249-awn9ayy6 237 9 ( ( -LRB- cord-001249-awn9ayy6 237 10 which which WDT cord-001249-awn9ayy6 237 11 , , , cord-001249-awn9ayy6 237 12 like like IN cord-001249-awn9ayy6 237 13 NSDV NSDV NNP cord-001249-awn9ayy6 237 14 , , , cord-001249-awn9ayy6 237 15 can can MD cord-001249-awn9ayy6 237 16 cause cause VB cord-001249-awn9ayy6 237 17 a a DT cord-001249-awn9ayy6 237 18 haemorrhagic haemorrhagic JJ cord-001249-awn9ayy6 237 19 fever fever NN cord-001249-awn9ayy6 237 20 ) ) -RRB- cord-001249-awn9ayy6 237 21 , , , cord-001249-awn9ayy6 237 22 increased increase VBN cord-001249-awn9ayy6 237 23 surface surface NN cord-001249-awn9ayy6 237 24 levels level NNS cord-001249-awn9ayy6 237 25 of of IN cord-001249-awn9ayy6 237 26 PDI PDI NNP cord-001249-awn9ayy6 237 27 in in IN cord-001249-awn9ayy6 237 28 infected infected JJ cord-001249-awn9ayy6 237 29 endothelial endothelial JJ cord-001249-awn9ayy6 237 30 cells cell NNS cord-001249-awn9ayy6 237 31 [ [ -LRB- cord-001249-awn9ayy6 237 32 86 86 CD cord-001249-awn9ayy6 237 33 ] ] -RRB- cord-001249-awn9ayy6 237 34 . . . cord-001249-awn9ayy6 238 1 As as IN cord-001249-awn9ayy6 238 2 in in IN cord-001249-awn9ayy6 238 3 our -PRON- PRP$ cord-001249-awn9ayy6 238 4 observations observation NNS cord-001249-awn9ayy6 238 5 on on IN cord-001249-awn9ayy6 238 6 NSDV NSDV NNP cord-001249-awn9ayy6 238 7 - - HYPH cord-001249-awn9ayy6 238 8 infected infect VBN cord-001249-awn9ayy6 238 9 cells cell NNS cord-001249-awn9ayy6 238 10 , , , cord-001249-awn9ayy6 238 11 total total JJ cord-001249-awn9ayy6 238 12 PDI PDI NNP cord-001249-awn9ayy6 238 13 levels level NNS cord-001249-awn9ayy6 238 14 were be VBD cord-001249-awn9ayy6 238 15 not not RB cord-001249-awn9ayy6 238 16 affected affect VBN cord-001249-awn9ayy6 238 17 in in IN cord-001249-awn9ayy6 238 18 DENV DENV NNP cord-001249-awn9ayy6 238 19 - - HYPH cord-001249-awn9ayy6 238 20 infected infect VBN cord-001249-awn9ayy6 238 21 cells cell NNS cord-001249-awn9ayy6 238 22 [ [ -LRB- cord-001249-awn9ayy6 238 23 86 86 CD cord-001249-awn9ayy6 238 24 ] ] -RRB- cord-001249-awn9ayy6 238 25 ; ; : cord-001249-awn9ayy6 238 26 at at IN cord-001249-awn9ayy6 238 27 present present NN cord-001249-awn9ayy6 238 28 it -PRON- PRP cord-001249-awn9ayy6 238 29 is be VBZ cord-001249-awn9ayy6 238 30 unknown unknown JJ cord-001249-awn9ayy6 238 31 whether whether IN cord-001249-awn9ayy6 238 32 DENV DENV NNP cord-001249-awn9ayy6 238 33 replication replication NN cord-001249-awn9ayy6 238 34 affects affect VBZ cord-001249-awn9ayy6 238 35 the the DT cord-001249-awn9ayy6 238 36 distribution distribution NN cord-001249-awn9ayy6 238 37 of of IN cord-001249-awn9ayy6 238 38 intracellular intracellular JJ cord-001249-awn9ayy6 238 39 PDI PDI NNP cord-001249-awn9ayy6 238 40 . . . cord-001249-awn9ayy6 239 1 Although although IN cord-001249-awn9ayy6 239 2 some some DT cord-001249-awn9ayy6 239 3 redistribution redistribution NN cord-001249-awn9ayy6 239 4 of of IN cord-001249-awn9ayy6 239 5 PDI PDI NNP cord-001249-awn9ayy6 239 6 was be VBD cord-001249-awn9ayy6 239 7 found find VBN cord-001249-awn9ayy6 239 8 in in IN cord-001249-awn9ayy6 239 9 BUNVinfected bunvinfecte VBN cord-001249-awn9ayy6 239 10 Vero Vero NNP cord-001249-awn9ayy6 239 11 cells cell NNS cord-001249-awn9ayy6 239 12 , , , cord-001249-awn9ayy6 239 13 these these DT cord-001249-awn9ayy6 239 14 changes change NNS cord-001249-awn9ayy6 239 15 most most RBS cord-001249-awn9ayy6 239 16 probably probably RB cord-001249-awn9ayy6 239 17 result result VBP cord-001249-awn9ayy6 239 18 from from IN cord-001249-awn9ayy6 239 19 fragmentation fragmentation NN cord-001249-awn9ayy6 239 20 of of IN cord-001249-awn9ayy6 239 21 the the DT cord-001249-awn9ayy6 239 22 ER ER NNP cord-001249-awn9ayy6 239 23 induced induce VBN cord-001249-awn9ayy6 239 24 by by IN cord-001249-awn9ayy6 239 25 the the DT cord-001249-awn9ayy6 239 26 virus virus NN cord-001249-awn9ayy6 239 27 and and CC cord-001249-awn9ayy6 240 1 this this DT cord-001249-awn9ayy6 240 2 effect effect NN cord-001249-awn9ayy6 240 3 on on IN cord-001249-awn9ayy6 240 4 PDI PDI NNP cord-001249-awn9ayy6 240 5 appears appear VBZ cord-001249-awn9ayy6 240 6 to to TO cord-001249-awn9ayy6 240 7 be be VB cord-001249-awn9ayy6 240 8 Vero Vero NNP cord-001249-awn9ayy6 240 9 - - HYPH cord-001249-awn9ayy6 240 10 specific specific JJ cord-001249-awn9ayy6 240 11 , , , cord-001249-awn9ayy6 240 12 since since IN cord-001249-awn9ayy6 240 13 these these DT cord-001249-awn9ayy6 240 14 changes change NNS cord-001249-awn9ayy6 240 15 have have VBP cord-001249-awn9ayy6 240 16 not not RB cord-001249-awn9ayy6 240 17 been be VBN cord-001249-awn9ayy6 240 18 observed observe VBN cord-001249-awn9ayy6 240 19 in in IN cord-001249-awn9ayy6 240 20 BUNV BUNV NNP cord-001249-awn9ayy6 240 21 - - HYPH cord-001249-awn9ayy6 240 22 infected infect VBN cord-001249-awn9ayy6 240 23 BHK21 BHK21 NNP cord-001249-awn9ayy6 240 24 cells cell NNS cord-001249-awn9ayy6 240 25 [ [ -LRB- cord-001249-awn9ayy6 240 26 79 79 CD cord-001249-awn9ayy6 240 27 ] ] -RRB- cord-001249-awn9ayy6 240 28 . . . cord-001249-awn9ayy6 241 1 No no DT cord-001249-awn9ayy6 241 2 effect effect NN cord-001249-awn9ayy6 241 3 on on IN cord-001249-awn9ayy6 241 4 PDI PDI NNP cord-001249-awn9ayy6 241 5 was be VBD cord-001249-awn9ayy6 241 6 detected detect VBN cord-001249-awn9ayy6 241 7 in in IN cord-001249-awn9ayy6 241 8 HTNV HTNV NNP cord-001249-awn9ayy6 241 9 - - HYPH cord-001249-awn9ayy6 241 10 infected infect VBN cord-001249-awn9ayy6 241 11 cells cell NNS cord-001249-awn9ayy6 241 12 [ [ -LRB- cord-001249-awn9ayy6 241 13 76 76 CD cord-001249-awn9ayy6 241 14 ] ] -RRB- cord-001249-awn9ayy6 241 15 . . . cord-001249-awn9ayy6 242 1 Redistribution redistribution NN cord-001249-awn9ayy6 242 2 of of IN cord-001249-awn9ayy6 242 3 soluble soluble JJ cord-001249-awn9ayy6 242 4 ER ER NNP cord-001249-awn9ayy6 242 5 proteins protein NNS cord-001249-awn9ayy6 242 6 PDI PDI NNP cord-001249-awn9ayy6 242 7 , , , cord-001249-awn9ayy6 242 8 ERp57 ERp57 NNS cord-001249-awn9ayy6 242 9 and and CC cord-001249-awn9ayy6 242 10 colligin colligin NNP cord-001249-awn9ayy6 242 11 ( ( -LRB- cord-001249-awn9ayy6 242 12 a a DT cord-001249-awn9ayy6 242 13 collagen collagen NN cord-001249-awn9ayy6 242 14 - - HYPH cord-001249-awn9ayy6 242 15 binding bind VBG cord-001249-awn9ayy6 242 16 protein protein NN cord-001249-awn9ayy6 242 17 ) ) -RRB- cord-001249-awn9ayy6 242 18 has have VBZ cord-001249-awn9ayy6 242 19 also also RB cord-001249-awn9ayy6 242 20 been be VBN cord-001249-awn9ayy6 242 21 found find VBN cord-001249-awn9ayy6 242 22 in in IN cord-001249-awn9ayy6 242 23 cells cell NNS cord-001249-awn9ayy6 242 24 infected infect VBN cord-001249-awn9ayy6 242 25 with with IN cord-001249-awn9ayy6 242 26 African african JJ cord-001249-awn9ayy6 242 27 swine swine NN cord-001249-awn9ayy6 242 28 fever fever NN cord-001249-awn9ayy6 242 29 virus virus NN cord-001249-awn9ayy6 242 30 ( ( -LRB- cord-001249-awn9ayy6 242 31 ASFV ASFV NNP cord-001249-awn9ayy6 242 32 ) ) -RRB- cord-001249-awn9ayy6 242 33 [ [ -LRB- cord-001249-awn9ayy6 242 34 87 87 CD cord-001249-awn9ayy6 242 35 , , , cord-001249-awn9ayy6 242 36 88 88 CD cord-001249-awn9ayy6 242 37 ] ] -RRB- cord-001249-awn9ayy6 242 38 , , , cord-001249-awn9ayy6 242 39 a a DT cord-001249-awn9ayy6 242 40 DNA dna NN cord-001249-awn9ayy6 242 41 virus virus NN cord-001249-awn9ayy6 242 42 which which WDT cord-001249-awn9ayy6 242 43 belongs belong VBZ cord-001249-awn9ayy6 242 44 to to IN cord-001249-awn9ayy6 242 45 the the DT cord-001249-awn9ayy6 242 46 family family NN cord-001249-awn9ayy6 242 47 Asfarviridae Asfarviridae NNP cord-001249-awn9ayy6 242 48 and and CC cord-001249-awn9ayy6 242 49 which which WDT cord-001249-awn9ayy6 242 50 causes cause VBZ cord-001249-awn9ayy6 242 51 a a DT cord-001249-awn9ayy6 242 52 severe severe JJ cord-001249-awn9ayy6 242 53 haemorrhagic haemorrhagic JJ cord-001249-awn9ayy6 242 54 disease disease NN cord-001249-awn9ayy6 242 55 in in IN cord-001249-awn9ayy6 242 56 domestic domestic JJ cord-001249-awn9ayy6 242 57 pigs pig NNS cord-001249-awn9ayy6 242 58 . . . cord-001249-awn9ayy6 243 1 In in IN cord-001249-awn9ayy6 243 2 ASFV ASFV NNP cord-001249-awn9ayy6 243 3 - - HYPH cord-001249-awn9ayy6 243 4 infected infect VBN cord-001249-awn9ayy6 243 5 cells cell NNS cord-001249-awn9ayy6 243 6 , , , cord-001249-awn9ayy6 243 7 PDI PDI NNP cord-001249-awn9ayy6 243 8 , , , cord-001249-awn9ayy6 243 9 ERp57 ERp57 NNS cord-001249-awn9ayy6 243 10 and and CC cord-001249-awn9ayy6 243 11 colligin colligin NNP cord-001249-awn9ayy6 243 12 were be VBD cord-001249-awn9ayy6 243 13 redistributed redistribute VBN cord-001249-awn9ayy6 243 14 from from IN cord-001249-awn9ayy6 243 15 the the DT cord-001249-awn9ayy6 243 16 ER ER NNP cord-001249-awn9ayy6 243 17 to to IN cord-001249-awn9ayy6 243 18 the the DT cord-001249-awn9ayy6 243 19 ERGIC ergic NN cord-001249-awn9ayy6 243 20 ( ( -LRB- cord-001249-awn9ayy6 243 21 which which WDT cord-001249-awn9ayy6 243 22 contained contain VBD cord-001249-awn9ayy6 243 23 the the DT cord-001249-awn9ayy6 243 24 ASFV ASFV NNP cord-001249-awn9ayy6 243 25 protein protein NN cord-001249-awn9ayy6 243 26 pXP124L pXP124L NNP cord-001249-awn9ayy6 243 27 ) ) -RRB- cord-001249-awn9ayy6 243 28 or or CC cord-001249-awn9ayy6 243 29 even even RB cord-001249-awn9ayy6 243 30 entirely entirely RB cord-001249-awn9ayy6 243 31 disappeared disappear VBD cord-001249-awn9ayy6 243 32 from from IN cord-001249-awn9ayy6 243 33 the the DT cord-001249-awn9ayy6 243 34 ER ER NNP cord-001249-awn9ayy6 243 35 , , , cord-001249-awn9ayy6 243 36 while while IN cord-001249-awn9ayy6 243 37 the the DT cord-001249-awn9ayy6 243 38 structure structure NN cord-001249-awn9ayy6 243 39 of of IN cord-001249-awn9ayy6 243 40 the the DT cord-001249-awn9ayy6 243 41 ER ER NNP cord-001249-awn9ayy6 243 42 was be VBD cord-001249-awn9ayy6 243 43 not not RB cord-001249-awn9ayy6 243 44 affected affect VBN cord-001249-awn9ayy6 243 45 [ [ -LRB- cord-001249-awn9ayy6 243 46 87 87 CD cord-001249-awn9ayy6 243 47 ] ] -RRB- cord-001249-awn9ayy6 243 48 . . . cord-001249-awn9ayy6 244 1 Total total JJ cord-001249-awn9ayy6 244 2 cellular cellular JJ cord-001249-awn9ayy6 244 3 levels level NNS cord-001249-awn9ayy6 244 4 of of IN cord-001249-awn9ayy6 244 5 PDI PDI NNP cord-001249-awn9ayy6 244 6 , , , cord-001249-awn9ayy6 244 7 ERp57 ERp57 NNS cord-001249-awn9ayy6 244 8 , , , cord-001249-awn9ayy6 244 9 and and CC cord-001249-awn9ayy6 244 10 colligin colligin NNP cord-001249-awn9ayy6 244 11 were be VBD cord-001249-awn9ayy6 244 12 not not RB cord-001249-awn9ayy6 244 13 affected affect VBN cord-001249-awn9ayy6 244 14 in in IN cord-001249-awn9ayy6 244 15 ASFV ASFV NNP cord-001249-awn9ayy6 244 16 - - HYPH cord-001249-awn9ayy6 244 17 infected infect VBN cord-001249-awn9ayy6 244 18 cells cell NNS cord-001249-awn9ayy6 244 19 and and CC cord-001249-awn9ayy6 244 20 redistribution redistribution NN cord-001249-awn9ayy6 244 21 of of IN cord-001249-awn9ayy6 244 22 these these DT cord-001249-awn9ayy6 244 23 ER ER NNP cord-001249-awn9ayy6 244 24 soluble soluble JJ cord-001249-awn9ayy6 244 25 proteins protein NNS cord-001249-awn9ayy6 244 26 was be VBD cord-001249-awn9ayy6 244 27 also also RB cord-001249-awn9ayy6 244 28 observed observe VBN cord-001249-awn9ayy6 244 29 in in IN cord-001249-awn9ayy6 244 30 cells cell NNS cord-001249-awn9ayy6 244 31 containing contain VBG cord-001249-awn9ayy6 244 32 plasmid plasmid NN cord-001249-awn9ayy6 244 33 - - HYPH cord-001249-awn9ayy6 244 34 expressed express VBN cord-001249-awn9ayy6 244 35 viral viral JJ cord-001249-awn9ayy6 244 36 protein protein NN cord-001249-awn9ayy6 244 37 pXP124L pXP124L NNP cord-001249-awn9ayy6 244 38 , , , cord-001249-awn9ayy6 244 39 suggesting suggest VBG cord-001249-awn9ayy6 244 40 that that IN cord-001249-awn9ayy6 244 41 these these DT cord-001249-awn9ayy6 244 42 ER ER NNP cord-001249-awn9ayy6 244 43 proteins protein NNS cord-001249-awn9ayy6 244 44 are be VBP cord-001249-awn9ayy6 244 45 not not RB cord-001249-awn9ayy6 244 46 physically physically RB cord-001249-awn9ayy6 244 47 redistributed redistribute VBN cord-001249-awn9ayy6 244 48 due due IN cord-001249-awn9ayy6 244 49 to to IN cord-001249-awn9ayy6 244 50 wrapping wrap VBG cord-001249-awn9ayy6 244 51 of of IN cord-001249-awn9ayy6 244 52 the the DT cord-001249-awn9ayy6 244 53 ASFV ASFV NNP cord-001249-awn9ayy6 244 54 progeny progeny NN cord-001249-awn9ayy6 244 55 virions virion NNS cord-001249-awn9ayy6 244 56 in in IN cord-001249-awn9ayy6 244 57 the the DT cord-001249-awn9ayy6 244 58 ER er NN cord-001249-awn9ayy6 244 59 but but CC cord-001249-awn9ayy6 244 60 due due IN cord-001249-awn9ayy6 244 61 to to IN cord-001249-awn9ayy6 244 62 a a DT cord-001249-awn9ayy6 244 63 specific specific JJ cord-001249-awn9ayy6 244 64 effect effect NN cord-001249-awn9ayy6 244 65 of of IN cord-001249-awn9ayy6 244 66 pXP124L. pXP124L. VBG cord-001249-awn9ayy6 244 67 Interestingly interestingly RB cord-001249-awn9ayy6 244 68 , , , cord-001249-awn9ayy6 244 69 all all PDT cord-001249-awn9ayy6 244 70 these these DT cord-001249-awn9ayy6 244 71 very very RB cord-001249-awn9ayy6 244 72 distinct distinct JJ cord-001249-awn9ayy6 244 73 viruses virus NNS cord-001249-awn9ayy6 244 74 ( ( -LRB- cord-001249-awn9ayy6 244 75 a a DT cord-001249-awn9ayy6 244 76 negativestrand negativestrand NN cord-001249-awn9ayy6 244 77 RNA RNA NNP cord-001249-awn9ayy6 244 78 virus virus NN cord-001249-awn9ayy6 244 79 , , , cord-001249-awn9ayy6 244 80 positive positive JJ cord-001249-awn9ayy6 244 81 - - HYPH cord-001249-awn9ayy6 244 82 strand strand NN cord-001249-awn9ayy6 244 83 RNA RNA NNP cord-001249-awn9ayy6 244 84 viruses virus NNS cord-001249-awn9ayy6 244 85 or or CC cord-001249-awn9ayy6 244 86 a a DT cord-001249-awn9ayy6 244 87 DNA dna NN cord-001249-awn9ayy6 244 88 virus virus NN cord-001249-awn9ayy6 244 89 ) ) -RRB- cord-001249-awn9ayy6 244 90 use use NN cord-001249-awn9ayy6 244 91 membranes membrane NNS cord-001249-awn9ayy6 244 92 of of IN cord-001249-awn9ayy6 244 93 the the DT cord-001249-awn9ayy6 244 94 secretory secretory JJ cord-001249-awn9ayy6 244 95 pathway pathway NN cord-001249-awn9ayy6 244 96 to to TO cord-001249-awn9ayy6 244 97 assemble assemble VB cord-001249-awn9ayy6 244 98 their -PRON- PRP$ cord-001249-awn9ayy6 244 99 virions virion NNS cord-001249-awn9ayy6 244 100 [ [ -LRB- cord-001249-awn9ayy6 244 101 35 35 CD cord-001249-awn9ayy6 244 102 , , , cord-001249-awn9ayy6 244 103 85 85 CD cord-001249-awn9ayy6 244 104 , , , cord-001249-awn9ayy6 244 105 89 89 CD cord-001249-awn9ayy6 244 106 , , , cord-001249-awn9ayy6 244 107 90 90 CD cord-001249-awn9ayy6 244 108 ] ] -RRB- cord-001249-awn9ayy6 244 109 , , , cord-001249-awn9ayy6 244 110 and and CC cord-001249-awn9ayy6 244 111 NSDV NSDV NNP cord-001249-awn9ayy6 244 112 , , , cord-001249-awn9ayy6 244 113 DENV DENV NNP cord-001249-awn9ayy6 244 114 and and CC cord-001249-awn9ayy6 244 115 ASFV ASFV NNP cord-001249-awn9ayy6 244 116 all all DT cord-001249-awn9ayy6 244 117 cause cause VBP cord-001249-awn9ayy6 244 118 haemorrhagic haemorrhagic JJ cord-001249-awn9ayy6 244 119 disease disease NN cord-001249-awn9ayy6 244 120 [ [ -LRB- cord-001249-awn9ayy6 244 121 2 2 CD cord-001249-awn9ayy6 244 122 , , , cord-001249-awn9ayy6 244 123 91 91 CD cord-001249-awn9ayy6 244 124 , , , cord-001249-awn9ayy6 244 125 92 92 CD cord-001249-awn9ayy6 244 126 ] ] -RRB- cord-001249-awn9ayy6 244 127 . . . cord-001249-awn9ayy6 245 1 The the DT cord-001249-awn9ayy6 245 2 underlying underlying JJ cord-001249-awn9ayy6 245 3 mechanism mechanism NN cord-001249-awn9ayy6 245 4 for for IN cord-001249-awn9ayy6 245 5 the the DT cord-001249-awn9ayy6 245 6 redistribution redistribution NN cord-001249-awn9ayy6 245 7 of of IN cord-001249-awn9ayy6 245 8 these these DT cord-001249-awn9ayy6 245 9 soluble soluble JJ cord-001249-awn9ayy6 245 10 ER ER NNP cord-001249-awn9ayy6 245 11 proteins protein NNS cord-001249-awn9ayy6 245 12 in in IN cord-001249-awn9ayy6 245 13 NSDV NSDV NNP cord-001249-awn9ayy6 245 14 infected infect VBN cord-001249-awn9ayy6 245 15 cells cell NNS cord-001249-awn9ayy6 245 16 remains remain VBZ cord-001249-awn9ayy6 245 17 to to TO cord-001249-awn9ayy6 245 18 be be VB cord-001249-awn9ayy6 245 19 determined determine VBN cord-001249-awn9ayy6 245 20 . . . cord-001249-awn9ayy6 246 1 PDI PDI NNP cord-001249-awn9ayy6 246 2 and and CC cord-001249-awn9ayy6 246 3 ERp57 ERp57 NNS cord-001249-awn9ayy6 246 4 , , , cord-001249-awn9ayy6 246 5 which which WDT cord-001249-awn9ayy6 246 6 contain contain VBP cord-001249-awn9ayy6 246 7 an an DT cord-001249-awn9ayy6 246 8 ER er NN cord-001249-awn9ayy6 246 9 retention retention NN cord-001249-awn9ayy6 246 10 motif motif NN cord-001249-awn9ayy6 246 11 at at IN cord-001249-awn9ayy6 246 12 their -PRON- PRP$ cord-001249-awn9ayy6 246 13 carboxy carboxy JJ cord-001249-awn9ayy6 246 14 - - HYPH cord-001249-awn9ayy6 246 15 terminus terminus NN cord-001249-awn9ayy6 246 16 , , , cord-001249-awn9ayy6 246 17 would would MD cord-001249-awn9ayy6 246 18 normally normally RB cord-001249-awn9ayy6 246 19 be be VB cord-001249-awn9ayy6 246 20 bound bind VBN cord-001249-awn9ayy6 246 21 to to IN cord-001249-awn9ayy6 246 22 KDEL KDEL NNP cord-001249-awn9ayy6 246 23 receptors receptor NNS cord-001249-awn9ayy6 246 24 in in IN cord-001249-awn9ayy6 246 25 the the DT cord-001249-awn9ayy6 246 26 ERGIC ERGIC NNP cord-001249-awn9ayy6 246 27 or or CC cord-001249-awn9ayy6 246 28 cisGolgi cisGolgi NNP cord-001249-awn9ayy6 246 29 and and CC cord-001249-awn9ayy6 246 30 recycled recycle VBD cord-001249-awn9ayy6 246 31 back back RB cord-001249-awn9ayy6 246 32 to to IN cord-001249-awn9ayy6 246 33 the the DT cord-001249-awn9ayy6 246 34 ER ER NNP cord-001249-awn9ayy6 246 35 , , , cord-001249-awn9ayy6 246 36 although although IN cord-001249-awn9ayy6 246 37 it -PRON- PRP cord-001249-awn9ayy6 246 38 is be VBZ cord-001249-awn9ayy6 246 39 known know VBN cord-001249-awn9ayy6 246 40 that that IN cord-001249-awn9ayy6 246 41 this this DT cord-001249-awn9ayy6 246 42 process process NN cord-001249-awn9ayy6 246 43 is be VBZ cord-001249-awn9ayy6 246 44 not not RB cord-001249-awn9ayy6 246 45 totally totally RB cord-001249-awn9ayy6 246 46 efficient efficient JJ cord-001249-awn9ayy6 246 47 , , , cord-001249-awn9ayy6 246 48 and and CC cord-001249-awn9ayy6 246 49 PDI PDI NNP cord-001249-awn9ayy6 246 50 at at IN cord-001249-awn9ayy6 246 51 least least JJS cord-001249-awn9ayy6 246 52 has have VBZ cord-001249-awn9ayy6 246 53 been be VBN cord-001249-awn9ayy6 246 54 observed observe VBN cord-001249-awn9ayy6 246 55 to to TO cord-001249-awn9ayy6 246 56 be be VB cord-001249-awn9ayy6 246 57 released release VBN cord-001249-awn9ayy6 246 58 from from IN cord-001249-awn9ayy6 246 59 some some DT cord-001249-awn9ayy6 246 60 cells cell NNS cord-001249-awn9ayy6 246 61 even even RB cord-001249-awn9ayy6 246 62 under under IN cord-001249-awn9ayy6 246 63 normal normal JJ cord-001249-awn9ayy6 246 64 conditions condition NNS cord-001249-awn9ayy6 246 65 [ [ -LRB- cord-001249-awn9ayy6 246 66 70 70 CD cord-001249-awn9ayy6 246 67 , , , cord-001249-awn9ayy6 246 68 74 74 CD cord-001249-awn9ayy6 246 69 ] ] -RRB- cord-001249-awn9ayy6 246 70 . . . cord-001249-awn9ayy6 247 1 What what WP cord-001249-awn9ayy6 247 2 is be VBZ cord-001249-awn9ayy6 247 3 more more JJR cord-001249-awn9ayy6 247 4 , , , cord-001249-awn9ayy6 247 5 its -PRON- PRP$ cord-001249-awn9ayy6 247 6 release release NN cord-001249-awn9ayy6 247 7 is be VBZ cord-001249-awn9ayy6 247 8 stimulated stimulate VBN cord-001249-awn9ayy6 247 9 in in IN cord-001249-awn9ayy6 247 10 activated activate VBN cord-001249-awn9ayy6 247 11 endothelial endothelial JJ cord-001249-awn9ayy6 247 12 cells cell NNS cord-001249-awn9ayy6 247 13 [ [ -LRB- cord-001249-awn9ayy6 247 14 93 93 CD cord-001249-awn9ayy6 247 15 , , , cord-001249-awn9ayy6 247 16 94 94 CD cord-001249-awn9ayy6 247 17 ] ] -RRB- cord-001249-awn9ayy6 247 18 . . . cord-001249-awn9ayy6 248 1 As as IN cord-001249-awn9ayy6 248 2 might may MD cord-001249-awn9ayy6 248 3 be be VB cord-001249-awn9ayy6 248 4 expected expect VBN cord-001249-awn9ayy6 248 5 due due IN cord-001249-awn9ayy6 248 6 to to IN cord-001249-awn9ayy6 248 7 the the DT cord-001249-awn9ayy6 248 8 generation generation NN cord-001249-awn9ayy6 248 9 of of IN cord-001249-awn9ayy6 248 10 large large JJ cord-001249-awn9ayy6 248 11 amounts amount NNS cord-001249-awn9ayy6 248 12 of of IN cord-001249-awn9ayy6 248 13 viral viral JJ cord-001249-awn9ayy6 248 14 glycoproteins glycoprotein NNS cord-001249-awn9ayy6 248 15 in in IN cord-001249-awn9ayy6 248 16 the the DT cord-001249-awn9ayy6 248 17 ER ER NNP cord-001249-awn9ayy6 248 18 , , , cord-001249-awn9ayy6 248 19 NSDV NSDV NNP cord-001249-awn9ayy6 248 20 induces induce VBZ cord-001249-awn9ayy6 248 21 the the DT cord-001249-awn9ayy6 248 22 unfolded unfolded JJ cord-001249-awn9ayy6 248 23 protein protein NN cord-001249-awn9ayy6 248 24 response response NN cord-001249-awn9ayy6 248 25 ( ( -LRB- cord-001249-awn9ayy6 248 26 UPR UPR NNP cord-001249-awn9ayy6 248 27 ) ) -RRB- cord-001249-awn9ayy6 248 28 in in IN cord-001249-awn9ayy6 248 29 infected infected JJ cord-001249-awn9ayy6 248 30 cells cell NNS cord-001249-awn9ayy6 248 31 ( ( -LRB- cord-001249-awn9ayy6 248 32 unpublished unpublished JJ cord-001249-awn9ayy6 248 33 observation observation NN cord-001249-awn9ayy6 248 34 ) ) -RRB- cord-001249-awn9ayy6 248 35 ; ; : cord-001249-awn9ayy6 248 36 however however RB cord-001249-awn9ayy6 248 37 , , , cord-001249-awn9ayy6 248 38 it -PRON- PRP cord-001249-awn9ayy6 248 39 has have VBZ cord-001249-awn9ayy6 248 40 been be VBN cord-001249-awn9ayy6 248 41 reported report VBN cord-001249-awn9ayy6 248 42 that that IN cord-001249-awn9ayy6 248 43 the the DT cord-001249-awn9ayy6 248 44 UPR UPR NNP cord-001249-awn9ayy6 248 45 appeared appear VBD cord-001249-awn9ayy6 248 46 to to TO cord-001249-awn9ayy6 248 47 diminish diminish VB cord-001249-awn9ayy6 248 48 PDI PDI NNP cord-001249-awn9ayy6 248 49 secretion secretion NN cord-001249-awn9ayy6 248 50 [ [ -LRB- cord-001249-awn9ayy6 248 51 74 74 CD cord-001249-awn9ayy6 248 52 ] ] -RRB- cord-001249-awn9ayy6 248 53 , , , cord-001249-awn9ayy6 248 54 so so CC cord-001249-awn9ayy6 248 55 it -PRON- PRP cord-001249-awn9ayy6 248 56 is be VBZ cord-001249-awn9ayy6 248 57 unlikely unlikely JJ cord-001249-awn9ayy6 248 58 that that IN cord-001249-awn9ayy6 248 59 virus virus NN cord-001249-awn9ayy6 248 60 - - HYPH cord-001249-awn9ayy6 248 61 induced induce VBN cord-001249-awn9ayy6 248 62 UPR UPR NNP cord-001249-awn9ayy6 248 63 is be VBZ cord-001249-awn9ayy6 248 64 the the DT cord-001249-awn9ayy6 248 65 cause cause NN cord-001249-awn9ayy6 248 66 of of IN cord-001249-awn9ayy6 248 67 the the DT cord-001249-awn9ayy6 248 68 PDI PDI NNP cord-001249-awn9ayy6 248 69 redistribution redistribution NN cord-001249-awn9ayy6 248 70 observed observe VBD cord-001249-awn9ayy6 248 71 . . . cord-001249-awn9ayy6 249 1 None none NN cord-001249-awn9ayy6 249 2 of of IN cord-001249-awn9ayy6 249 3 the the DT cord-001249-awn9ayy6 249 4 predicted predict VBN cord-001249-awn9ayy6 249 5 glycoproteins glycoprotein NNS cord-001249-awn9ayy6 249 6 of of IN cord-001249-awn9ayy6 249 7 NSDV NSDV NNP cord-001249-awn9ayy6 249 8 have have VBP cord-001249-awn9ayy6 249 9 a a DT cord-001249-awn9ayy6 249 10 C c NN cord-001249-awn9ayy6 249 11 - - HYPH cord-001249-awn9ayy6 249 12 terminal terminal NN cord-001249-awn9ayy6 249 13 sequence sequence NN cord-001249-awn9ayy6 249 14 recognised recognise VBN cord-001249-awn9ayy6 249 15 by by IN cord-001249-awn9ayy6 249 16 KDEL KDEL NNP cord-001249-awn9ayy6 249 17 receptors receptor NNS cord-001249-awn9ayy6 249 18 , , , cord-001249-awn9ayy6 249 19 so so IN cord-001249-awn9ayy6 249 20 the the DT cord-001249-awn9ayy6 249 21 viral viral JJ cord-001249-awn9ayy6 249 22 proteins protein NNS cord-001249-awn9ayy6 249 23 can can MD cord-001249-awn9ayy6 249 24 not not RB cord-001249-awn9ayy6 249 25 be be VB cord-001249-awn9ayy6 249 26 competing compete VBG cord-001249-awn9ayy6 249 27 with with IN cord-001249-awn9ayy6 249 28 the the DT cord-001249-awn9ayy6 249 29 host host NN cord-001249-awn9ayy6 249 30 cell cell NN cord-001249-awn9ayy6 249 31 proteins protein NNS cord-001249-awn9ayy6 249 32 for for IN cord-001249-awn9ayy6 249 33 KDEL KDEL NNP cord-001249-awn9ayy6 249 34 receptors receptor NNS cord-001249-awn9ayy6 249 35 , , , cord-001249-awn9ayy6 249 36 unlike unlike IN cord-001249-awn9ayy6 249 37 the the DT cord-001249-awn9ayy6 249 38 case case NN cord-001249-awn9ayy6 249 39 of of IN cord-001249-awn9ayy6 249 40 ASFV ASFV NNP cord-001249-awn9ayy6 249 41 pXP124L pXP124L NNP cord-001249-awn9ayy6 249 42 , , , cord-001249-awn9ayy6 249 43 the the DT cord-001249-awn9ayy6 249 44 effects effect NNS cord-001249-awn9ayy6 249 45 of of IN cord-001249-awn9ayy6 249 46 which which WDT cord-001249-awn9ayy6 249 47 on on IN cord-001249-awn9ayy6 249 48 ER ER NNP cord-001249-awn9ayy6 249 49 proteins protein NNS cord-001249-awn9ayy6 249 50 is be VBZ cord-001249-awn9ayy6 249 51 dependent dependent JJ cord-001249-awn9ayy6 249 52 on on IN cord-001249-awn9ayy6 249 53 its -PRON- PRP$ cord-001249-awn9ayy6 249 54 own own JJ cord-001249-awn9ayy6 249 55 C c NN cord-001249-awn9ayy6 249 56 - - HYPH cord-001249-awn9ayy6 249 57 terminal terminal NN cord-001249-awn9ayy6 249 58 KDEL KDEL NNP cord-001249-awn9ayy6 249 59 motif motif NN cord-001249-awn9ayy6 249 60 [ [ -LRB- cord-001249-awn9ayy6 249 61 87 87 CD cord-001249-awn9ayy6 249 62 ] ] -RRB- cord-001249-awn9ayy6 249 63 . . . cord-001249-awn9ayy6 250 1 Interaction interaction NN cord-001249-awn9ayy6 250 2 of of IN cord-001249-awn9ayy6 250 3 ER ER NNP cord-001249-awn9ayy6 250 4 chaperones chaperone NNS cord-001249-awn9ayy6 250 5 ( ( -LRB- cord-001249-awn9ayy6 250 6 e.g. e.g. RB cord-001249-awn9ayy6 250 7 PDI PDI NNP cord-001249-awn9ayy6 250 8 , , , cord-001249-awn9ayy6 250 9 BiP BiP NNP cord-001249-awn9ayy6 250 10 , , , cord-001249-awn9ayy6 250 11 calnexin calnexin NNP cord-001249-awn9ayy6 250 12 and/or and/or CC cord-001249-awn9ayy6 250 13 calreticulin calreticulin NNP cord-001249-awn9ayy6 250 14 ) ) -RRB- cord-001249-awn9ayy6 250 15 with with IN cord-001249-awn9ayy6 250 16 viral viral JJ cord-001249-awn9ayy6 250 17 glycoproteins glycoprotein NNS cord-001249-awn9ayy6 250 18 during during IN cord-001249-awn9ayy6 250 19 their -PRON- PRP$ cord-001249-awn9ayy6 250 20 folding folding NN cord-001249-awn9ayy6 250 21 in in IN cord-001249-awn9ayy6 250 22 the the DT cord-001249-awn9ayy6 250 23 ER ER NNP cord-001249-awn9ayy6 250 24 would would MD cord-001249-awn9ayy6 250 25 be be VB cord-001249-awn9ayy6 250 26 expected expect VBN cord-001249-awn9ayy6 250 27 and and CC cord-001249-awn9ayy6 250 28 has have VBZ cord-001249-awn9ayy6 250 29 been be VBN cord-001249-awn9ayy6 250 30 shown show VBN cord-001249-awn9ayy6 250 31 for for IN cord-001249-awn9ayy6 250 32 HTNV HTNV NNP cord-001249-awn9ayy6 250 33 and and CC cord-001249-awn9ayy6 250 34 UUKV UUKV NNP cord-001249-awn9ayy6 250 35 [ [ -LRB- cord-001249-awn9ayy6 250 36 95 95 CD cord-001249-awn9ayy6 250 37 ] ] -RRB- cord-001249-awn9ayy6 251 1 [ [ -LRB- cord-001249-awn9ayy6 251 2 96 96 CD cord-001249-awn9ayy6 251 3 ] ] -RRB- cord-001249-awn9ayy6 251 4 [ [ -LRB- cord-001249-awn9ayy6 251 5 97 97 CD cord-001249-awn9ayy6 251 6 ] ] -RRB- cord-001249-awn9ayy6 251 7 . . . cord-001249-awn9ayy6 252 1 It -PRON- PRP cord-001249-awn9ayy6 252 2 remains remain VBZ cord-001249-awn9ayy6 252 3 to to TO cord-001249-awn9ayy6 252 4 be be VB cord-001249-awn9ayy6 252 5 determined determine VBN cord-001249-awn9ayy6 252 6 why why WRB cord-001249-awn9ayy6 252 7 the the DT cord-001249-awn9ayy6 252 8 PDI PDI NNP cord-001249-awn9ayy6 252 9 is be VBZ cord-001249-awn9ayy6 252 10 not not RB cord-001249-awn9ayy6 252 11 recycled recycle VBN cord-001249-awn9ayy6 252 12 from from IN cord-001249-awn9ayy6 252 13 the the DT cord-001249-awn9ayy6 252 14 ERGIC ERGIC NNP cord-001249-awn9ayy6 252 15 or or CC cord-001249-awn9ayy6 252 16 cisGolgi cisGolgi NNP cord-001249-awn9ayy6 252 17 in in IN cord-001249-awn9ayy6 252 18 this this DT cord-001249-awn9ayy6 252 19 case case NN cord-001249-awn9ayy6 252 20 , , , cord-001249-awn9ayy6 252 21 and and CC cord-001249-awn9ayy6 252 22 why why WRB cord-001249-awn9ayy6 252 23 only only RB cord-001249-awn9ayy6 252 24 the the DT cord-001249-awn9ayy6 252 25 PreGn PreGn NNP cord-001249-awn9ayy6 252 26 protein protein NN cord-001249-awn9ayy6 252 27 appears appear VBZ cord-001249-awn9ayy6 252 28 to to TO cord-001249-awn9ayy6 252 29 be be VB cord-001249-awn9ayy6 252 30 involved involve VBN cord-001249-awn9ayy6 252 31 in in IN cord-001249-awn9ayy6 252 32 PDI PDI NNP cord-001249-awn9ayy6 252 33 distribution distribution NN cord-001249-awn9ayy6 252 34 and and CC cord-001249-awn9ayy6 252 35 not not RB cord-001249-awn9ayy6 252 36 PreGc pregc NN cord-001249-awn9ayy6 252 37 . . . cord-001249-awn9ayy6 253 1 Figure figure NN cord-001249-awn9ayy6 253 2 9 9 CD cord-001249-awn9ayy6 253 3 . . . cord-001249-awn9ayy6 254 1 The the DT cord-001249-awn9ayy6 254 2 effect effect NN cord-001249-awn9ayy6 254 3 of of IN cord-001249-awn9ayy6 254 4 NSDV NSDV NNP cord-001249-awn9ayy6 254 5 glycoprotein glycoprotein NN cord-001249-awn9ayy6 254 6 expression expression NN cord-001249-awn9ayy6 254 7 on on IN cord-001249-awn9ayy6 254 8 PDI PDI NNP cord-001249-awn9ayy6 254 9 . . . cord-001249-awn9ayy6 255 1 Vero Vero NNP cord-001249-awn9ayy6 255 2 cells cell NNS cord-001249-awn9ayy6 255 3 were be VBD cord-001249-awn9ayy6 255 4 transfected transfecte VBN cord-001249-awn9ayy6 255 5 with with IN cord-001249-awn9ayy6 255 6 1 1 CD cord-001249-awn9ayy6 255 7 mg mg NN cord-001249-awn9ayy6 255 8 of of IN cord-001249-awn9ayy6 255 9 pCAGGs_MCSII_PreGn_V5 pcaggs_mcsii_pregn_v5 NN cord-001249-awn9ayy6 255 10 ( ( -LRB- cord-001249-awn9ayy6 255 11 a a FW cord-001249-awn9ayy6 255 12 - - HYPH cord-001249-awn9ayy6 255 13 c c NN cord-001249-awn9ayy6 255 14 and and CC cord-001249-awn9ayy6 255 15 j j NNP cord-001249-awn9ayy6 255 16 - - HYPH cord-001249-awn9ayy6 255 17 l l NNP cord-001249-awn9ayy6 255 18 ) ) -RRB- cord-001249-awn9ayy6 255 19 , , , cord-001249-awn9ayy6 255 20 pCAGGs_MCSII_PreGc_V5 pCAGGs_MCSII_PreGc_V5 NNP cord-001249-awn9ayy6 255 21 ( ( -LRB- cord-001249-awn9ayy6 255 22 d d NNP cord-001249-awn9ayy6 255 23 - - HYPH cord-001249-awn9ayy6 255 24 f f NNP cord-001249-awn9ayy6 255 25 ) ) -RRB- cord-001249-awn9ayy6 255 26 or or CC cord-001249-awn9ayy6 255 27 pCAGGs_MCSII_NS pCAGGs_MCSII_NS NNP cord-001249-awn9ayy6 255 28 M M NNP cord-001249-awn9ayy6 255 29 _ _ NNP cord-001249-awn9ayy6 255 30 V5 V5 NNP cord-001249-awn9ayy6 255 31 ( ( -LRB- cord-001249-awn9ayy6 255 32 g g NN cord-001249-awn9ayy6 255 33 - - : cord-001249-awn9ayy6 255 34 i i NNP cord-001249-awn9ayy6 255 35 ) ) -RRB- cord-001249-awn9ayy6 255 36 . . . cord-001249-awn9ayy6 256 1 After after IN cord-001249-awn9ayy6 256 2 24 24 CD cord-001249-awn9ayy6 256 3 h h NN cord-001249-awn9ayy6 256 4 , , , cord-001249-awn9ayy6 256 5 cells cell NNS cord-001249-awn9ayy6 256 6 were be VBD cord-001249-awn9ayy6 256 7 fixed fix VBN cord-001249-awn9ayy6 256 8 with with IN cord-001249-awn9ayy6 256 9 3 3 CD cord-001249-awn9ayy6 256 10 % % NN cord-001249-awn9ayy6 257 1 PFA PFA NNP cord-001249-awn9ayy6 257 2 followed follow VBD cord-001249-awn9ayy6 257 3 by by IN cord-001249-awn9ayy6 257 4 ice ice NN cord-001249-awn9ayy6 257 5 - - HYPH cord-001249-awn9ayy6 257 6 cold cold JJ cord-001249-awn9ayy6 257 7 methanol methanol NN cord-001249-awn9ayy6 257 8 , , , cord-001249-awn9ayy6 257 9 and and CC cord-001249-awn9ayy6 257 10 were be VBD cord-001249-awn9ayy6 257 11 stained stain VBN cord-001249-awn9ayy6 257 12 using use VBG cord-001249-awn9ayy6 257 13 mouse mouse NN cord-001249-awn9ayy6 257 14 anti anti JJ cord-001249-awn9ayy6 257 15 - - NNP cord-001249-awn9ayy6 257 16 PDI pdi JJ cord-001249-awn9ayy6 257 17 ( ( -LRB- cord-001249-awn9ayy6 257 18 a a RB cord-001249-awn9ayy6 257 19 - - : cord-001249-awn9ayy6 257 20 i i NN cord-001249-awn9ayy6 257 21 ) ) -RRB- cord-001249-awn9ayy6 257 22 or or CC cord-001249-awn9ayy6 257 23 anti anti JJ cord-001249-awn9ayy6 257 24 - - JJ cord-001249-awn9ayy6 257 25 calnexin calnexin JJ cord-001249-awn9ayy6 257 26 ( ( -LRB- cord-001249-awn9ayy6 257 27 j j NNP cord-001249-awn9ayy6 257 28 - - HYPH cord-001249-awn9ayy6 257 29 l l NNP cord-001249-awn9ayy6 257 30 ) ) -RRB- cord-001249-awn9ayy6 257 31 antibodies antibody NNS cord-001249-awn9ayy6 257 32 followed follow VBN cord-001249-awn9ayy6 257 33 by by IN cord-001249-awn9ayy6 257 34 Alexa Alexa NNP cord-001249-awn9ayy6 257 35 Fluor Fluor NNP cord-001249-awn9ayy6 257 36 568 568 CD cord-001249-awn9ayy6 257 37 goat goat NN cord-001249-awn9ayy6 257 38 anti anti JJ cord-001249-awn9ayy6 257 39 - - JJ cord-001249-awn9ayy6 257 40 mouse mouse JJ cord-001249-awn9ayy6 257 41 IgG IgG NNP cord-001249-awn9ayy6 257 42 ( ( -LRB- cord-001249-awn9ayy6 257 43 red red NNP cord-001249-awn9ayy6 257 44 ) ) -RRB- cord-001249-awn9ayy6 257 45 . . . cord-001249-awn9ayy6 258 1 Plasmid Plasmid NNP cord-001249-awn9ayy6 258 2 - - HYPH cord-001249-awn9ayy6 258 3 expressed express VBN cord-001249-awn9ayy6 258 4 proteins protein NNS cord-001249-awn9ayy6 258 5 were be VBD cord-001249-awn9ayy6 258 6 visualised visualise VBN cord-001249-awn9ayy6 258 7 with with IN cord-001249-awn9ayy6 258 8 mouse mouse NN cord-001249-awn9ayy6 258 9 anti anti JJ cord-001249-awn9ayy6 258 10 - - JJ cord-001249-awn9ayy6 258 11 V5 v5 JJ cord-001249-awn9ayy6 258 12 antibody antibody NN cord-001249-awn9ayy6 258 13 conjugated conjugate VBN cord-001249-awn9ayy6 258 14 to to IN cord-001249-awn9ayy6 258 15 Alexa Alexa NNP cord-001249-awn9ayy6 258 16 Fluor Fluor NNP cord-001249-awn9ayy6 258 17 488 488 CD cord-001249-awn9ayy6 258 18 ( ( -LRB- cord-001249-awn9ayy6 258 19 green green NNP cord-001249-awn9ayy6 258 20 ) ) -RRB- cord-001249-awn9ayy6 258 21 . . . cord-001249-awn9ayy6 259 1 Nuclei nucleus NNS cord-001249-awn9ayy6 259 2 were be VBD cord-001249-awn9ayy6 259 3 counterstained counterstaine VBN cord-001249-awn9ayy6 259 4 using use VBG cord-001249-awn9ayy6 259 5 DAPI DAPI NNP cord-001249-awn9ayy6 259 6 ( ( -LRB- cord-001249-awn9ayy6 259 7 blue blue JJ cord-001249-awn9ayy6 259 8 ) ) -RRB- cord-001249-awn9ayy6 259 9 . . . cord-001249-awn9ayy6 260 1 Bars bar NNS cord-001249-awn9ayy6 260 2 correspond correspond VBP cord-001249-awn9ayy6 260 3 to to IN cord-001249-awn9ayy6 260 4 40 40 CD cord-001249-awn9ayy6 260 5 mm mm NNS cord-001249-awn9ayy6 260 6 . . . cord-001249-awn9ayy6 261 1 Arrows arrow NNS cord-001249-awn9ayy6 261 2 in in IN cord-001249-awn9ayy6 261 3 a a DT cord-001249-awn9ayy6 261 4 - - HYPH cord-001249-awn9ayy6 261 5 b b NN cord-001249-awn9ayy6 261 6 and and CC cord-001249-awn9ayy6 261 7 j j NNP cord-001249-awn9ayy6 261 8 - - HYPH cord-001249-awn9ayy6 261 9 k k NNP cord-001249-awn9ayy6 261 10 indicate indicate VBP cord-001249-awn9ayy6 261 11 cells cell NNS cord-001249-awn9ayy6 261 12 expressing express VBG cord-001249-awn9ayy6 261 13 PreGn pregn PRP cord-001249-awn9ayy6 261 14 . . . cord-001249-awn9ayy6 262 1 doi:10.1371/ doi:10.1371/ NNP cord-001249-awn9ayy6 263 1 journal.pone.0094656.g009 journal.pone.0094656.g009 NNP cord-001249-awn9ayy6 264 1 It -PRON- PRP cord-001249-awn9ayy6 264 2 is be VBZ cord-001249-awn9ayy6 264 3 possible possible JJ cord-001249-awn9ayy6 264 4 that that WDT cord-001249-awn9ayy6 264 5 bound bind VBN cord-001249-awn9ayy6 264 6 PreGn pregn PRP cord-001249-awn9ayy6 264 7 makes make VBZ cord-001249-awn9ayy6 264 8 PDI PDI NNP cord-001249-awn9ayy6 264 9 not not RB cord-001249-awn9ayy6 264 10 available available JJ cord-001249-awn9ayy6 264 11 for for IN cord-001249-awn9ayy6 264 12 KDEL KDEL NNP cord-001249-awn9ayy6 264 13 receptor receptor NN cord-001249-awn9ayy6 264 14 . . . cord-001249-awn9ayy6 265 1 While while IN cord-001249-awn9ayy6 265 2 we -PRON- PRP cord-001249-awn9ayy6 265 3 have have VBP cord-001249-awn9ayy6 265 4 been be VBN cord-001249-awn9ayy6 265 5 unable unable JJ cord-001249-awn9ayy6 265 6 to to TO cord-001249-awn9ayy6 265 7 detect detect VB cord-001249-awn9ayy6 265 8 a a DT cord-001249-awn9ayy6 265 9 separate separate JJ cord-001249-awn9ayy6 265 10 mucin mucin NN cord-001249-awn9ayy6 265 11 - - HYPH cord-001249-awn9ayy6 265 12 like like JJ cord-001249-awn9ayy6 265 13 protein protein NN cord-001249-awn9ayy6 265 14 / / SYM cord-001249-awn9ayy6 265 15 GP38 GP38 NNP cord-001249-awn9ayy6 265 16 protein protein NN cord-001249-awn9ayy6 265 17 , , , cord-001249-awn9ayy6 265 18 such such JJ cord-001249-awn9ayy6 265 19 as as IN cord-001249-awn9ayy6 265 20 that that DT cord-001249-awn9ayy6 265 21 cleaved cleave VBD cord-001249-awn9ayy6 265 22 from from IN cord-001249-awn9ayy6 265 23 the the DT cord-001249-awn9ayy6 265 24 CCHFV CCHFV NNP cord-001249-awn9ayy6 265 25 PreGn pregn NN cord-001249-awn9ayy6 265 26 protein protein NN cord-001249-awn9ayy6 265 27 by by IN cord-001249-awn9ayy6 265 28 an an DT cord-001249-awn9ayy6 265 29 SKI-1-like ski-1-like NN cord-001249-awn9ayy6 265 30 protease protease NN cord-001249-awn9ayy6 265 31 in in IN cord-001249-awn9ayy6 265 32 the the DT cord-001249-awn9ayy6 265 33 cisGolgi cisgolgi JJ cord-001249-awn9ayy6 265 34 [ [ -LRB- cord-001249-awn9ayy6 265 35 57 57 CD cord-001249-awn9ayy6 265 36 , , , cord-001249-awn9ayy6 265 37 58 58 CD cord-001249-awn9ayy6 265 38 ] ] -RRB- cord-001249-awn9ayy6 265 39 , , , cord-001249-awn9ayy6 265 40 in in IN cord-001249-awn9ayy6 265 41 NSDV NSDV NNP cord-001249-awn9ayy6 265 42 - - HYPH cord-001249-awn9ayy6 265 43 infected infect VBN cord-001249-awn9ayy6 265 44 cells cell NNS cord-001249-awn9ayy6 265 45 , , , cord-001249-awn9ayy6 265 46 it -PRON- PRP cord-001249-awn9ayy6 265 47 may may MD cord-001249-awn9ayy6 265 48 be be VB cord-001249-awn9ayy6 265 49 that that DT cord-001249-awn9ayy6 265 50 final final JJ cord-001249-awn9ayy6 265 51 folding folding NN cord-001249-awn9ayy6 265 52 of of IN cord-001249-awn9ayy6 265 53 the the DT cord-001249-awn9ayy6 265 54 NSDV NSDV NNP cord-001249-awn9ayy6 265 55 ( ( -LRB- cord-001249-awn9ayy6 265 56 Pre)Gn Pre)Gn NNP cord-001249-awn9ayy6 265 57 occurs occur VBZ cord-001249-awn9ayy6 265 58 late late RB cord-001249-awn9ayy6 265 59 in in IN cord-001249-awn9ayy6 265 60 the the DT cord-001249-awn9ayy6 265 61 exit exit NN cord-001249-awn9ayy6 265 62 pathway pathway NN cord-001249-awn9ayy6 265 63 of of IN cord-001249-awn9ayy6 265 64 the the DT cord-001249-awn9ayy6 265 65 virion virion NN cord-001249-awn9ayy6 265 66 so so IN cord-001249-awn9ayy6 265 67 that that IN cord-001249-awn9ayy6 265 68 PDI PDI NNP cord-001249-awn9ayy6 265 69 is be VBZ cord-001249-awn9ayy6 265 70 still still RB cord-001249-awn9ayy6 265 71 required require VBN cord-001249-awn9ayy6 265 72 for for IN cord-001249-awn9ayy6 265 73 protein protein NN cord-001249-awn9ayy6 265 74 maturation maturation NN cord-001249-awn9ayy6 265 75 , , , cord-001249-awn9ayy6 265 76 even even RB cord-001249-awn9ayy6 265 77 after after IN cord-001249-awn9ayy6 265 78 virion virion NN cord-001249-awn9ayy6 265 79 assembly assembly NN cord-001249-awn9ayy6 265 80 . . . cord-001249-awn9ayy6 266 1 Further further JJ cord-001249-awn9ayy6 266 2 maturation maturation NN cord-001249-awn9ayy6 266 3 of of IN cord-001249-awn9ayy6 266 4 newly newly RB cord-001249-awn9ayy6 266 5 synthesised synthesise VBN cord-001249-awn9ayy6 266 6 virions virion NNS cord-001249-awn9ayy6 266 7 in in IN cord-001249-awn9ayy6 266 8 post post JJ cord-001249-awn9ayy6 266 9 - - JJ cord-001249-awn9ayy6 266 10 transGolgi transgolgi JJ cord-001249-awn9ayy6 266 11 network network NN cord-001249-awn9ayy6 266 12 has have VBZ cord-001249-awn9ayy6 266 13 been be VBN cord-001249-awn9ayy6 266 14 observed observe VBN cord-001249-awn9ayy6 266 15 in in IN cord-001249-awn9ayy6 266 16 BUNV BUNV NNP cord-001249-awn9ayy6 266 17 - - HYPH cord-001249-awn9ayy6 266 18 infected infect VBN cord-001249-awn9ayy6 266 19 cells cell NNS cord-001249-awn9ayy6 266 20 [ [ -LRB- cord-001249-awn9ayy6 266 21 79 79 CD cord-001249-awn9ayy6 266 22 , , , cord-001249-awn9ayy6 266 23 80 80 CD cord-001249-awn9ayy6 266 24 ] ] -RRB- cord-001249-awn9ayy6 266 25 , , , cord-001249-awn9ayy6 266 26 supporting support VBG cord-001249-awn9ayy6 266 27 the the DT cord-001249-awn9ayy6 266 28 possibility possibility NN cord-001249-awn9ayy6 266 29 that that IN cord-001249-awn9ayy6 266 30 PDI PDI NNP cord-001249-awn9ayy6 266 31 might may MD cord-001249-awn9ayy6 266 32 play play VB cord-001249-awn9ayy6 266 33 a a DT cord-001249-awn9ayy6 266 34 role role NN cord-001249-awn9ayy6 266 35 in in IN cord-001249-awn9ayy6 266 36 late late JJ cord-001249-awn9ayy6 266 37 maturation maturation NN cord-001249-awn9ayy6 266 38 of of IN cord-001249-awn9ayy6 266 39 NSDV NSDV NNP cord-001249-awn9ayy6 266 40 . . . cord-001249-awn9ayy6 267 1 Late late JJ cord-001249-awn9ayy6 267 2 maturation maturation NN cord-001249-awn9ayy6 267 3 , , , cord-001249-awn9ayy6 267 4 or or CC cord-001249-awn9ayy6 267 5 even even RB cord-001249-awn9ayy6 267 6 virion virion NN cord-001249-awn9ayy6 267 7 - - HYPH cord-001249-awn9ayy6 267 8 bound bind VBN cord-001249-awn9ayy6 267 9 PDI PDI NNP cord-001249-awn9ayy6 267 10 , , , cord-001249-awn9ayy6 267 11 may may MD cord-001249-awn9ayy6 267 12 be be VB cord-001249-awn9ayy6 267 13 required require VBN cord-001249-awn9ayy6 267 14 to to TO cord-001249-awn9ayy6 267 15 prevent prevent VB cord-001249-awn9ayy6 267 16 fusion fusion NN cord-001249-awn9ayy6 267 17 of of IN cord-001249-awn9ayy6 267 18 the the DT cord-001249-awn9ayy6 267 19 viral viral JJ cord-001249-awn9ayy6 267 20 membrane membrane NN cord-001249-awn9ayy6 267 21 with with IN cord-001249-awn9ayy6 267 22 the the DT cord-001249-awn9ayy6 267 23 membrane membrane NN cord-001249-awn9ayy6 267 24 of of IN cord-001249-awn9ayy6 267 25 the the DT cord-001249-awn9ayy6 267 26 secretory secretory JJ cord-001249-awn9ayy6 267 27 compartments compartment NNS cord-001249-awn9ayy6 267 28 during during IN cord-001249-awn9ayy6 267 29 virus virus NN cord-001249-awn9ayy6 267 30 egress egress NN cord-001249-awn9ayy6 267 31 . . . cord-001249-awn9ayy6 268 1 Fusion fusion NN cord-001249-awn9ayy6 268 2 of of IN cord-001249-awn9ayy6 268 3 the the DT cord-001249-awn9ayy6 268 4 CCHFV CCHFV NNP cord-001249-awn9ayy6 268 5 virion virion NN cord-001249-awn9ayy6 268 6 with with IN cord-001249-awn9ayy6 268 7 the the DT cord-001249-awn9ayy6 268 8 endosomal endosomal NNP cord-001249-awn9ayy6 268 9 membrane membrane NN cord-001249-awn9ayy6 268 10 is be VBZ cord-001249-awn9ayy6 268 11 induced induce VBN cord-001249-awn9ayy6 268 12 at at IN cord-001249-awn9ayy6 268 13 pH pH NNP cord-001249-awn9ayy6 268 14 5.5 5.5 CD cord-001249-awn9ayy6 268 15 to to IN cord-001249-awn9ayy6 268 16 6.0 6.0 CD cord-001249-awn9ayy6 268 17 [ [ -LRB- cord-001249-awn9ayy6 268 18 98 98 CD cord-001249-awn9ayy6 268 19 ] ] -RRB- cord-001249-awn9ayy6 268 20 , , , cord-001249-awn9ayy6 268 21 which which WDT cord-001249-awn9ayy6 268 22 is be VBZ cord-001249-awn9ayy6 268 23 within within IN cord-001249-awn9ayy6 268 24 the the DT cord-001249-awn9ayy6 268 25 range range NN cord-001249-awn9ayy6 268 26 of of IN cord-001249-awn9ayy6 268 27 pH pH NNP cord-001249-awn9ayy6 268 28 observed observe VBN cord-001249-awn9ayy6 268 29 in in IN cord-001249-awn9ayy6 268 30 the the DT cord-001249-awn9ayy6 268 31 lumen lumen NN cord-001249-awn9ayy6 268 32 of of IN cord-001249-awn9ayy6 268 33 post post JJ cord-001249-awn9ayy6 268 34 - - JJ cord-001249-awn9ayy6 268 35 Golgi Golgi NNP cord-001249-awn9ayy6 268 36 compartments compartment NNS cord-001249-awn9ayy6 268 37 ( ( -LRB- cord-001249-awn9ayy6 268 38 reviewed review VBN cord-001249-awn9ayy6 268 39 in in IN cord-001249-awn9ayy6 268 40 [ [ -LRB- cord-001249-awn9ayy6 268 41 99 99 CD cord-001249-awn9ayy6 268 42 ] ] -RRB- cord-001249-awn9ayy6 268 43 ) ) -RRB- cord-001249-awn9ayy6 268 44 , , , cord-001249-awn9ayy6 268 45 so so IN cord-001249-awn9ayy6 268 46 a a DT cord-001249-awn9ayy6 268 47 system system NN cord-001249-awn9ayy6 268 48 which which WDT cord-001249-awn9ayy6 268 49 prevented prevent VBD cord-001249-awn9ayy6 268 50 fusion fusion NN cord-001249-awn9ayy6 268 51 of of IN cord-001249-awn9ayy6 268 52 newly newly RB cord-001249-awn9ayy6 268 53 synthesised synthesise VBN cord-001249-awn9ayy6 268 54 virions virion NNS cord-001249-awn9ayy6 268 55 with with IN cord-001249-awn9ayy6 268 56 intracellular intracellular JJ cord-001249-awn9ayy6 268 57 membranes membrane NNS cord-001249-awn9ayy6 268 58 prior prior RB cord-001249-awn9ayy6 268 59 to to IN cord-001249-awn9ayy6 268 60 virus virus NN cord-001249-awn9ayy6 268 61 egress egress NN cord-001249-awn9ayy6 268 62 would would MD cord-001249-awn9ayy6 268 63 be be VB cord-001249-awn9ayy6 268 64 of of IN cord-001249-awn9ayy6 268 65 benefit benefit NN cord-001249-awn9ayy6 268 66 . . . cord-001249-awn9ayy6 269 1 An an DT cord-001249-awn9ayy6 269 2 alternative alternative NN cord-001249-awn9ayy6 269 3 is be VBZ cord-001249-awn9ayy6 269 4 that that IN cord-001249-awn9ayy6 269 5 the the DT cord-001249-awn9ayy6 269 6 virus virus NN cord-001249-awn9ayy6 269 7 benefits benefit NNS cord-001249-awn9ayy6 269 8 by by IN cord-001249-awn9ayy6 269 9 inducing induce VBG cord-001249-awn9ayy6 269 10 PDI PDI NNP cord-001249-awn9ayy6 269 11 release release NN cord-001249-awn9ayy6 269 12 from from IN cord-001249-awn9ayy6 269 13 cells cell NNS cord-001249-awn9ayy6 269 14 because because IN cord-001249-awn9ayy6 269 15 the the DT cord-001249-awn9ayy6 269 16 PDI PDI NNP cord-001249-awn9ayy6 269 17 is be VBZ cord-001249-awn9ayy6 269 18 required require VBN cord-001249-awn9ayy6 269 19 for for IN cord-001249-awn9ayy6 269 20 virus virus NN cord-001249-awn9ayy6 269 21 entry entry NN cord-001249-awn9ayy6 269 22 . . . cord-001249-awn9ayy6 270 1 Extracellular Extracellular NNP cord-001249-awn9ayy6 270 2 PDI PDI NNP cord-001249-awn9ayy6 270 3 , , , cord-001249-awn9ayy6 270 4 with with IN cord-001249-awn9ayy6 270 5 its -PRON- PRP$ cord-001249-awn9ayy6 270 6 potential potential NN cord-001249-awn9ayy6 270 7 for for IN cord-001249-awn9ayy6 270 8 rearranging rearrange VBG cord-001249-awn9ayy6 270 9 disulphide disulphide JJ cord-001249-awn9ayy6 270 10 bonds bond NNS cord-001249-awn9ayy6 270 11 within within IN cord-001249-awn9ayy6 270 12 viral viral JJ cord-001249-awn9ayy6 270 13 glycoproteins glycoprotein NNS cord-001249-awn9ayy6 270 14 , , , cord-001249-awn9ayy6 270 15 is be VBZ cord-001249-awn9ayy6 270 16 thought think VBN cord-001249-awn9ayy6 270 17 to to TO cord-001249-awn9ayy6 270 18 aid aid VB cord-001249-awn9ayy6 270 19 during during IN cord-001249-awn9ayy6 270 20 entry entry NN cord-001249-awn9ayy6 270 21 of of IN cord-001249-awn9ayy6 270 22 several several JJ cord-001249-awn9ayy6 270 23 viruses virus NNS cord-001249-awn9ayy6 270 24 [ [ -LRB- cord-001249-awn9ayy6 270 25 86 86 CD cord-001249-awn9ayy6 270 26 , , , cord-001249-awn9ayy6 270 27 [ [ -LRB- cord-001249-awn9ayy6 270 28 100 100 CD cord-001249-awn9ayy6 270 29 ] ] -RRB- cord-001249-awn9ayy6 270 30 [ [ -LRB- cord-001249-awn9ayy6 270 31 101 101 CD cord-001249-awn9ayy6 270 32 ] ] -RRB- cord-001249-awn9ayy6 270 33 [ [ -LRB- cord-001249-awn9ayy6 270 34 102 102 CD cord-001249-awn9ayy6 270 35 ] ] -RRB- cord-001249-awn9ayy6 270 36 [ [ -LRB- cord-001249-awn9ayy6 270 37 103 103 CD cord-001249-awn9ayy6 270 38 ] ] -RRB- cord-001249-awn9ayy6 270 39 [ [ -LRB- cord-001249-awn9ayy6 270 40 104 104 CD cord-001249-awn9ayy6 270 41 ] ] -RRB- cord-001249-awn9ayy6 270 42 [ [ -LRB- cord-001249-awn9ayy6 270 43 105 105 CD cord-001249-awn9ayy6 270 44 ] ] -RRB- cord-001249-awn9ayy6 270 45 ; ; : cord-001249-awn9ayy6 270 46 free free JJ cord-001249-awn9ayy6 270 47 thiol thiol NN cord-001249-awn9ayy6 270 48 groups group NNS cord-001249-awn9ayy6 270 49 in in IN cord-001249-awn9ayy6 270 50 the the DT cord-001249-awn9ayy6 270 51 F F NNP cord-001249-awn9ayy6 270 52 fusion fusion NN cord-001249-awn9ayy6 270 53 protein protein NN cord-001249-awn9ayy6 270 54 of of IN cord-001249-awn9ayy6 270 55 Newcastle Newcastle NNP cord-001249-awn9ayy6 270 56 disease disease NN cord-001249-awn9ayy6 270 57 virus virus NN cord-001249-awn9ayy6 270 58 or or CC cord-001249-awn9ayy6 270 59 the the DT cord-001249-awn9ayy6 270 60 gp120 gp120 NNP cord-001249-awn9ayy6 270 61 subunit subunit NN cord-001249-awn9ayy6 270 62 of of IN cord-001249-awn9ayy6 270 63 the the DT cord-001249-awn9ayy6 270 64 human human JJ cord-001249-awn9ayy6 270 65 immunodeficiency immunodeficiency NN cord-001249-awn9ayy6 270 66 virus virus NN cord-001249-awn9ayy6 270 67 Env Env NNP cord-001249-awn9ayy6 270 68 glycoprotein glycoprotein NN cord-001249-awn9ayy6 270 69 are be VBP cord-001249-awn9ayy6 270 70 critical critical JJ cord-001249-awn9ayy6 270 71 for for IN cord-001249-awn9ayy6 270 72 virus virus NN cord-001249-awn9ayy6 270 73 entry entry NN cord-001249-awn9ayy6 270 74 and and CC cord-001249-awn9ayy6 270 75 their -PRON- PRP$ cord-001249-awn9ayy6 270 76 presence presence NN cord-001249-awn9ayy6 270 77 is be VBZ cord-001249-awn9ayy6 270 78 thought think VBN cord-001249-awn9ayy6 270 79 to to TO cord-001249-awn9ayy6 270 80 be be VB cord-001249-awn9ayy6 270 81 mediated mediate VBN cord-001249-awn9ayy6 270 82 by by IN cord-001249-awn9ayy6 270 83 cell cell NN cord-001249-awn9ayy6 270 84 surface surface NN cord-001249-awn9ayy6 270 85 PDI PDI NNP cord-001249-awn9ayy6 270 86 [ [ -LRB- cord-001249-awn9ayy6 270 87 100 100 CD cord-001249-awn9ayy6 270 88 ] ] -RRB- cord-001249-awn9ayy6 270 89 [ [ -LRB- cord-001249-awn9ayy6 270 90 101 101 CD cord-001249-awn9ayy6 270 91 ] ] -RRB- cord-001249-awn9ayy6 270 92 [ [ -LRB- cord-001249-awn9ayy6 270 93 102 102 CD cord-001249-awn9ayy6 270 94 ] ] -RRB- cord-001249-awn9ayy6 270 95 [ [ -LRB- cord-001249-awn9ayy6 270 96 103 103 CD cord-001249-awn9ayy6 270 97 ] ] -RRB- cord-001249-awn9ayy6 270 98 [ [ -LRB- cord-001249-awn9ayy6 270 99 104 104 CD cord-001249-awn9ayy6 270 100 ] ] -RRB- cord-001249-awn9ayy6 270 101 [ [ -LRB- cord-001249-awn9ayy6 270 102 105 105 CD cord-001249-awn9ayy6 270 103 ] ] -RRB- cord-001249-awn9ayy6 270 104 . . . cord-001249-awn9ayy6 271 1 Recent recent JJ cord-001249-awn9ayy6 271 2 data datum NNS cord-001249-awn9ayy6 271 3 suggest suggest VBP cord-001249-awn9ayy6 271 4 that that IN cord-001249-awn9ayy6 271 5 free free JJ cord-001249-awn9ayy6 271 6 thiol thiol NN cord-001249-awn9ayy6 271 7 groups group NNS cord-001249-awn9ayy6 271 8 at at IN cord-001249-awn9ayy6 271 9 the the DT cord-001249-awn9ayy6 271 10 surface surface NN cord-001249-awn9ayy6 271 11 of of IN cord-001249-awn9ayy6 271 12 hantaviruses hantaviruse NNS cord-001249-awn9ayy6 271 13 might may MD cord-001249-awn9ayy6 271 14 also also RB cord-001249-awn9ayy6 271 15 play play VB cord-001249-awn9ayy6 271 16 a a DT cord-001249-awn9ayy6 271 17 role role NN cord-001249-awn9ayy6 271 18 in in IN cord-001249-awn9ayy6 271 19 infectivity infectivity NN cord-001249-awn9ayy6 271 20 of of IN cord-001249-awn9ayy6 271 21 these these DT cord-001249-awn9ayy6 271 22 viruses virus NNS cord-001249-awn9ayy6 271 23 [ [ -LRB- cord-001249-awn9ayy6 271 24 106 106 CD cord-001249-awn9ayy6 271 25 ] ] -RRB- cord-001249-awn9ayy6 271 26 . . . cord-001249-awn9ayy6 272 1 Whether whether IN cord-001249-awn9ayy6 272 2 NSDV NSDV NNP cord-001249-awn9ayy6 272 3 derives derive VBZ cord-001249-awn9ayy6 272 4 a a DT cord-001249-awn9ayy6 272 5 selectable selectable JJ cord-001249-awn9ayy6 272 6 advantage advantage NN cord-001249-awn9ayy6 272 7 from from IN cord-001249-awn9ayy6 272 8 the the DT cord-001249-awn9ayy6 272 9 redistribution redistribution NN cord-001249-awn9ayy6 272 10 of of IN cord-001249-awn9ayy6 272 11 PDI PDI NNP cord-001249-awn9ayy6 272 12 and and CC cord-001249-awn9ayy6 272 13 ERp57 ERp57 NNS cord-001249-awn9ayy6 272 14 in in IN cord-001249-awn9ayy6 272 15 infected infected JJ cord-001249-awn9ayy6 272 16 cells cell NNS cord-001249-awn9ayy6 272 17 or or CC cord-001249-awn9ayy6 272 18 this this DT cord-001249-awn9ayy6 272 19 redistribution redistribution NN cord-001249-awn9ayy6 272 20 is be VBZ cord-001249-awn9ayy6 272 21 a a DT cord-001249-awn9ayy6 272 22 bystander bystander NN cord-001249-awn9ayy6 272 23 effect effect NN cord-001249-awn9ayy6 272 24 of of IN cord-001249-awn9ayy6 272 25 the the DT cord-001249-awn9ayy6 272 26 NSDV NSDV NNP cord-001249-awn9ayy6 272 27 infection infection NN cord-001249-awn9ayy6 272 28 , , , cord-001249-awn9ayy6 272 29 extracellular extracellular JJ cord-001249-awn9ayy6 272 30 PDI PDI NNP cord-001249-awn9ayy6 272 31 may may MD cord-001249-awn9ayy6 272 32 contribute contribute VB cord-001249-awn9ayy6 272 33 to to IN cord-001249-awn9ayy6 272 34 the the DT cord-001249-awn9ayy6 272 35 pathogenicity pathogenicity NN cord-001249-awn9ayy6 272 36 observed observe VBN cord-001249-awn9ayy6 272 37 during during IN cord-001249-awn9ayy6 272 38 NSDV NSDV NNP cord-001249-awn9ayy6 272 39 infection infection NN cord-001249-awn9ayy6 272 40 . . . cord-001249-awn9ayy6 273 1 Extracellular Extracellular NNP cord-001249-awn9ayy6 273 2 PDI PDI NNP cord-001249-awn9ayy6 273 3 can can MD cord-001249-awn9ayy6 273 4 still still RB cord-001249-awn9ayy6 273 5 reduce reduce VB cord-001249-awn9ayy6 273 6 , , , cord-001249-awn9ayy6 273 7 oxidise oxidise VB cord-001249-awn9ayy6 273 8 and and CC cord-001249-awn9ayy6 273 9 rearrange rearrange VB cord-001249-awn9ayy6 273 10 disulphide disulphide JJ cord-001249-awn9ayy6 273 11 bonds bond NNS cord-001249-awn9ayy6 273 12 and and CC cord-001249-awn9ayy6 273 13 therefore therefore RB cord-001249-awn9ayy6 273 14 activate activate VBP cord-001249-awn9ayy6 273 15 molecules molecule NNS cord-001249-awn9ayy6 273 16 with with IN cord-001249-awn9ayy6 273 17 which which WDT cord-001249-awn9ayy6 273 18 it -PRON- PRP cord-001249-awn9ayy6 273 19 interacts interact VBZ cord-001249-awn9ayy6 273 20 at at IN cord-001249-awn9ayy6 273 21 the the DT cord-001249-awn9ayy6 273 22 cell cell NN cord-001249-awn9ayy6 273 23 surface surface NN cord-001249-awn9ayy6 273 24 or or CC cord-001249-awn9ayy6 273 25 in in IN cord-001249-awn9ayy6 273 26 the the DT cord-001249-awn9ayy6 273 27 extracellular extracellular JJ cord-001249-awn9ayy6 273 28 space space NN cord-001249-awn9ayy6 273 29 ( ( -LRB- cord-001249-awn9ayy6 273 30 reviewed review VBN cord-001249-awn9ayy6 273 31 in in IN cord-001249-awn9ayy6 273 32 [ [ -LRB- cord-001249-awn9ayy6 273 33 107 107 CD cord-001249-awn9ayy6 273 34 , , , cord-001249-awn9ayy6 273 35 108 108 CD cord-001249-awn9ayy6 273 36 ] ] -RRB- cord-001249-awn9ayy6 273 37 ) ) -RRB- cord-001249-awn9ayy6 273 38 . . . cord-001249-awn9ayy6 274 1 Extracellular Extracellular NNP cord-001249-awn9ayy6 274 2 PDI PDI NNP cord-001249-awn9ayy6 274 3 has have VBZ cord-001249-awn9ayy6 274 4 been be VBN cord-001249-awn9ayy6 274 5 shown show VBN cord-001249-awn9ayy6 274 6 to to TO cord-001249-awn9ayy6 274 7 activate activate VB cord-001249-awn9ayy6 274 8 b1 b1 NN cord-001249-awn9ayy6 274 9 and and CC cord-001249-awn9ayy6 274 10 b3 b3 NN cord-001249-awn9ayy6 274 11 subunit subunit NN cord-001249-awn9ayy6 274 12 - - HYPH cord-001249-awn9ayy6 274 13 containing contain VBG cord-001249-awn9ayy6 274 14 integrins integrin NNS cord-001249-awn9ayy6 274 15 on on IN cord-001249-awn9ayy6 274 16 endothelial endothelial JJ cord-001249-awn9ayy6 274 17 cells cell NNS cord-001249-awn9ayy6 274 18 and and CC cord-001249-awn9ayy6 274 19 platelets platelet NNS cord-001249-awn9ayy6 274 20 [ [ -LRB- cord-001249-awn9ayy6 274 21 86 86 CD cord-001249-awn9ayy6 274 22 , , , cord-001249-awn9ayy6 274 23 93 93 CD cord-001249-awn9ayy6 274 24 , , , cord-001249-awn9ayy6 274 25 109 109 CD cord-001249-awn9ayy6 274 26 ] ] -RRB- cord-001249-awn9ayy6 274 27 . . . cord-001249-awn9ayy6 275 1 Integrins integrin NNS cord-001249-awn9ayy6 275 2 are be VBP cord-001249-awn9ayy6 275 3 known know VBN cord-001249-awn9ayy6 275 4 to to TO cord-001249-awn9ayy6 275 5 take take VB cord-001249-awn9ayy6 275 6 part part NN cord-001249-awn9ayy6 275 7 in in IN cord-001249-awn9ayy6 275 8 the the DT cord-001249-awn9ayy6 275 9 cell cell NN cord-001249-awn9ayy6 275 10 - - HYPH cord-001249-awn9ayy6 275 11 cell cell NN cord-001249-awn9ayy6 275 12 interactions interaction NNS cord-001249-awn9ayy6 275 13 promoting promote VBG cord-001249-awn9ayy6 275 14 cell cell NN cord-001249-awn9ayy6 275 15 migration migration NN cord-001249-awn9ayy6 275 16 and and CC cord-001249-awn9ayy6 275 17 vasodilation vasodilation NN cord-001249-awn9ayy6 275 18 ( ( -LRB- cord-001249-awn9ayy6 275 19 reviewed review VBN cord-001249-awn9ayy6 275 20 in in IN cord-001249-awn9ayy6 275 21 [ [ -LRB- cord-001249-awn9ayy6 275 22 110 110 CD cord-001249-awn9ayy6 275 23 ] ] -RRB- cord-001249-awn9ayy6 275 24 ) ) -RRB- cord-001249-awn9ayy6 275 25 , , , cord-001249-awn9ayy6 275 26 processes process NNS cord-001249-awn9ayy6 275 27 which which WDT cord-001249-awn9ayy6 275 28 are be VBP cord-001249-awn9ayy6 275 29 enhanced enhance VBN cord-001249-awn9ayy6 275 30 during during IN cord-001249-awn9ayy6 275 31 viral viral JJ cord-001249-awn9ayy6 275 32 haemorrhagic haemorrhagic JJ cord-001249-awn9ayy6 275 33 fevers fever NNS cord-001249-awn9ayy6 275 34 and and CC cord-001249-awn9ayy6 275 35 which which WDT cord-001249-awn9ayy6 275 36 contribute contribute VBP cord-001249-awn9ayy6 275 37 to to IN cord-001249-awn9ayy6 275 38 the the DT cord-001249-awn9ayy6 275 39 haemorrhagic haemorrhagic JJ cord-001249-awn9ayy6 275 40 nature nature NN cord-001249-awn9ayy6 275 41 of of IN cord-001249-awn9ayy6 275 42 the the DT cord-001249-awn9ayy6 275 43 pathogenesis pathogenesis NN cord-001249-awn9ayy6 275 44 ( ( -LRB- cord-001249-awn9ayy6 275 45 reviewed review VBN cord-001249-awn9ayy6 275 46 in in IN cord-001249-awn9ayy6 275 47 [ [ -LRB- cord-001249-awn9ayy6 275 48 111 111 CD cord-001249-awn9ayy6 275 49 ] ] -RRB- cord-001249-awn9ayy6 275 50 ) ) -RRB- cord-001249-awn9ayy6 276 1 e.g. e.g. RB cord-001249-awn9ayy6 276 2 DENV DENV NNP cord-001249-awn9ayy6 276 3 - - HYPH cord-001249-awn9ayy6 276 4 induced induce VBN cord-001249-awn9ayy6 276 5 translocation translocation NN cord-001249-awn9ayy6 276 6 of of IN cord-001249-awn9ayy6 276 7 PDI PDI NNP cord-001249-awn9ayy6 276 8 to to IN cord-001249-awn9ayy6 276 9 the the DT cord-001249-awn9ayy6 276 10 cells cell NNS cord-001249-awn9ayy6 276 11 surface surface NN cord-001249-awn9ayy6 276 12 has have VBZ cord-001249-awn9ayy6 276 13 been be VBN cord-001249-awn9ayy6 276 14 shown show VBN cord-001249-awn9ayy6 276 15 to to TO cord-001249-awn9ayy6 276 16 activate activate VB cord-001249-awn9ayy6 276 17 integrins integrin NNS cord-001249-awn9ayy6 276 18 on on IN cord-001249-awn9ayy6 276 19 infected infected JJ cord-001249-awn9ayy6 276 20 endothelial endothelial JJ cord-001249-awn9ayy6 276 21 cells cell NNS cord-001249-awn9ayy6 276 22 [ [ -LRB- cord-001249-awn9ayy6 276 23 86 86 CD cord-001249-awn9ayy6 276 24 ] ] -RRB- cord-001249-awn9ayy6 276 25 . . . cord-001249-awn9ayy6 277 1 Secreted secreted JJ cord-001249-awn9ayy6 277 2 PDI PDI NNP cord-001249-awn9ayy6 277 3 could could MD cord-001249-awn9ayy6 277 4 also also RB cord-001249-awn9ayy6 277 5 activate activate VB cord-001249-awn9ayy6 277 6 integrins integrin NNS cord-001249-awn9ayy6 277 7 at at IN cord-001249-awn9ayy6 277 8 the the DT cord-001249-awn9ayy6 277 9 surface surface NN cord-001249-awn9ayy6 277 10 of of IN cord-001249-awn9ayy6 277 11 various various JJ cord-001249-awn9ayy6 277 12 uninfected uninfected JJ cord-001249-awn9ayy6 277 13 cells cell NNS cord-001249-awn9ayy6 277 14 , , , cord-001249-awn9ayy6 277 15 including include VBG cord-001249-awn9ayy6 277 16 endothelial endothelial JJ cord-001249-awn9ayy6 277 17 cells cell NNS cord-001249-awn9ayy6 277 18 and and CC cord-001249-awn9ayy6 277 19 platelets platelet NNS cord-001249-awn9ayy6 277 20 , , , cord-001249-awn9ayy6 277 21 leading lead VBG cord-001249-awn9ayy6 277 22 to to IN cord-001249-awn9ayy6 277 23 increased increase VBN cord-001249-awn9ayy6 277 24 vasodilatation vasodilatation NN cord-001249-awn9ayy6 277 25 and and CC cord-001249-awn9ayy6 277 26 coagulopathy coagulopathy NN cord-001249-awn9ayy6 277 27 . . . cord-001249-awn9ayy6 278 1 Extracellular Extracellular NNP cord-001249-awn9ayy6 278 2 PDI PDI NNP cord-001249-awn9ayy6 278 3 has have VBZ cord-001249-awn9ayy6 278 4 been be VBN cord-001249-awn9ayy6 278 5 shown show VBN cord-001249-awn9ayy6 278 6 to to TO cord-001249-awn9ayy6 278 7 activate activate VB cord-001249-awn9ayy6 278 8 tissue tissue NN cord-001249-awn9ayy6 278 9 factor factor NN cord-001249-awn9ayy6 278 10 ( ( -LRB- cord-001249-awn9ayy6 278 11 TF TF NNP cord-001249-awn9ayy6 278 12 ) ) -RRB- cord-001249-awn9ayy6 278 13 [ [ -LRB- cord-001249-awn9ayy6 278 14 112 112 CD cord-001249-awn9ayy6 278 15 ] ] -RRB- cord-001249-awn9ayy6 278 16 , , , cord-001249-awn9ayy6 278 17 inducing induce VBG cord-001249-awn9ayy6 278 18 intravascular intravascular JJ cord-001249-awn9ayy6 278 19 coagulation coagulation NN cord-001249-awn9ayy6 278 20 , , , cord-001249-awn9ayy6 278 21 which which WDT cord-001249-awn9ayy6 278 22 may may MD cord-001249-awn9ayy6 278 23 subsequently subsequently RB cord-001249-awn9ayy6 278 24 lead lead VB cord-001249-awn9ayy6 278 25 to to IN cord-001249-awn9ayy6 278 26 disseminated disseminate VBN cord-001249-awn9ayy6 278 27 intravascular intravascular JJ cord-001249-awn9ayy6 278 28 coagulation coagulation NN cord-001249-awn9ayy6 278 29 ( ( -LRB- cord-001249-awn9ayy6 278 30 DIC DIC NNP cord-001249-awn9ayy6 278 31 ) ) -RRB- cord-001249-awn9ayy6 278 32 through through IN cord-001249-awn9ayy6 278 33 depletion depletion NN cord-001249-awn9ayy6 278 34 of of IN cord-001249-awn9ayy6 278 35 platelets platelet NNS cord-001249-awn9ayy6 278 36 and and CC cord-001249-awn9ayy6 278 37 clotting clotting NN cord-001249-awn9ayy6 278 38 factors factor NNS cord-001249-awn9ayy6 278 39 , , , cord-001249-awn9ayy6 278 40 resulting result VBG cord-001249-awn9ayy6 278 41 in in IN cord-001249-awn9ayy6 278 42 haemorrhaging haemorrhage VBG cord-001249-awn9ayy6 278 43 [ [ -LRB- cord-001249-awn9ayy6 278 44 113 113 CD cord-001249-awn9ayy6 278 45 ] ] -RRB- cord-001249-awn9ayy6 278 46 [ [ -LRB- cord-001249-awn9ayy6 278 47 114 114 CD cord-001249-awn9ayy6 278 48 ] ] -RRB- cord-001249-awn9ayy6 278 49 [ [ -LRB- cord-001249-awn9ayy6 278 50 115 115 CD cord-001249-awn9ayy6 278 51 ] ] -RRB- cord-001249-awn9ayy6 278 52 [ [ -LRB- cord-001249-awn9ayy6 278 53 116 116 CD cord-001249-awn9ayy6 278 54 ] ] -RRB- cord-001249-awn9ayy6 279 1 ; ; : cord-001249-awn9ayy6 279 2 DIC DIC NNP cord-001249-awn9ayy6 279 3 has have VBZ cord-001249-awn9ayy6 279 4 been be VBN cord-001249-awn9ayy6 279 5 observed observe VBN cord-001249-awn9ayy6 279 6 in in IN cord-001249-awn9ayy6 279 7 many many JJ cord-001249-awn9ayy6 279 8 CCHF CCHF NNP cord-001249-awn9ayy6 279 9 patients patient NNS cord-001249-awn9ayy6 279 10 [ [ -LRB- cord-001249-awn9ayy6 279 11 31 31 CD cord-001249-awn9ayy6 279 12 ] ] -RRB- cord-001249-awn9ayy6 280 1 [ [ -LRB- cord-001249-awn9ayy6 280 2 32 32 CD cord-001249-awn9ayy6 280 3 ] ] -RRB- cord-001249-awn9ayy6 280 4 [ [ -LRB- cord-001249-awn9ayy6 280 5 33 33 CD cord-001249-awn9ayy6 280 6 ] ] -RRB- cord-001249-awn9ayy6 280 7 117 117 CD cord-001249-awn9ayy6 280 8 ] ] -RRB- cord-001249-awn9ayy6 280 9 . . . cord-001249-awn9ayy6 281 1 Finally finally RB cord-001249-awn9ayy6 281 2 , , , cord-001249-awn9ayy6 281 3 since since IN cord-001249-awn9ayy6 281 4 ERp57 ERp57 NNS cord-001249-awn9ayy6 281 5 ( ( -LRB- cord-001249-awn9ayy6 281 6 and and CC cord-001249-awn9ayy6 281 7 probably probably RB cord-001249-awn9ayy6 281 8 PDI PDI NNP cord-001249-awn9ayy6 281 9 ) ) -RRB- cord-001249-awn9ayy6 281 10 takes take VBZ cord-001249-awn9ayy6 281 11 part part NN cord-001249-awn9ayy6 281 12 in in IN cord-001249-awn9ayy6 281 13 the the DT cord-001249-awn9ayy6 281 14 folding folding NN cord-001249-awn9ayy6 281 15 of of IN cord-001249-awn9ayy6 281 16 the the DT cord-001249-awn9ayy6 281 17 major major JJ cord-001249-awn9ayy6 281 18 histocompatibility histocompatibility NN cord-001249-awn9ayy6 281 19 complex complex NN cord-001249-awn9ayy6 281 20 ( ( -LRB- cord-001249-awn9ayy6 281 21 MHC MHC NNP cord-001249-awn9ayy6 281 22 ) ) -RRB- cord-001249-awn9ayy6 281 23 class class NN cord-001249-awn9ayy6 281 24 I i NN cord-001249-awn9ayy6 281 25 and and CC cord-001249-awn9ayy6 281 26 therefore therefore RB cord-001249-awn9ayy6 281 27 assists assist VBZ cord-001249-awn9ayy6 281 28 in in IN cord-001249-awn9ayy6 281 29 facilitating facilitate VBG cord-001249-awn9ayy6 281 30 immune immune JJ cord-001249-awn9ayy6 281 31 surveillance surveillance NN cord-001249-awn9ayy6 281 32 and and CC cord-001249-awn9ayy6 281 33 elimination elimination NN cord-001249-awn9ayy6 281 34 of of IN cord-001249-awn9ayy6 281 35 virus virus NN cord-001249-awn9ayy6 281 36 - - HYPH cord-001249-awn9ayy6 281 37 infected infect VBN cord-001249-awn9ayy6 281 38 cells cell NNS cord-001249-awn9ayy6 281 39 [ [ -LRB- cord-001249-awn9ayy6 281 40 118,119,120 118,119,120 CD cord-001249-awn9ayy6 281 41 , , , cord-001249-awn9ayy6 281 42 ] ] -RRB- cord-001249-awn9ayy6 281 43 viruses virus NNS cord-001249-awn9ayy6 281 44 which which WDT cord-001249-awn9ayy6 281 45 translocate translocate VBP cord-001249-awn9ayy6 281 46 these these DT cord-001249-awn9ayy6 281 47 ER ER NNP cord-001249-awn9ayy6 281 48 chaperones chaperone NNS cord-001249-awn9ayy6 281 49 from from IN cord-001249-awn9ayy6 281 50 the the DT cord-001249-awn9ayy6 281 51 ER ER NNP cord-001249-awn9ayy6 281 52 may may MD cord-001249-awn9ayy6 281 53 avoid avoid VB cord-001249-awn9ayy6 281 54 adaptive adaptive JJ cord-001249-awn9ayy6 281 55 immune immune JJ cord-001249-awn9ayy6 281 56 responses response NNS cord-001249-awn9ayy6 281 57 , , , cord-001249-awn9ayy6 281 58 although although IN cord-001249-awn9ayy6 281 59 down down IN cord-001249-awn9ayy6 281 60 - - HYPH cord-001249-awn9ayy6 281 61 regulation regulation NN cord-001249-awn9ayy6 281 62 of of IN cord-001249-awn9ayy6 281 63 MHC MHC NNP cord-001249-awn9ayy6 281 64 class class NN cord-001249-awn9ayy6 281 65 I -PRON- PRP cord-001249-awn9ayy6 281 66 has have VBZ cord-001249-awn9ayy6 281 67 not not RB cord-001249-awn9ayy6 281 68 yet yet RB cord-001249-awn9ayy6 281 69 been be VBN cord-001249-awn9ayy6 281 70 described describe VBN cord-001249-awn9ayy6 281 71 for for IN cord-001249-awn9ayy6 281 72 nairovirus nairovirus NNP cord-001249-awn9ayy6 281 73 - - HYPH cord-001249-awn9ayy6 281 74 infected infect VBN cord-001249-awn9ayy6 281 75 cells cell NNS cord-001249-awn9ayy6 281 76 . . . cord-001249-awn9ayy6 282 1 In in IN cord-001249-awn9ayy6 282 2 this this DT cord-001249-awn9ayy6 282 3 study study NN cord-001249-awn9ayy6 282 4 we -PRON- PRP cord-001249-awn9ayy6 282 5 have have VBP cord-001249-awn9ayy6 282 6 demonstrated demonstrate VBN cord-001249-awn9ayy6 282 7 that that IN cord-001249-awn9ayy6 282 8 NSDV NSDV NNP cord-001249-awn9ayy6 282 9 induces induce VBZ cord-001249-awn9ayy6 282 10 the the DT cord-001249-awn9ayy6 282 11 redistribution redistribution NN cord-001249-awn9ayy6 282 12 of of IN cord-001249-awn9ayy6 282 13 soluble soluble JJ cord-001249-awn9ayy6 282 14 ER ER NNP cord-001249-awn9ayy6 282 15 oxidoreductases oxidoreductase NNS cord-001249-awn9ayy6 282 16 , , , cord-001249-awn9ayy6 282 17 specifically specifically RB cord-001249-awn9ayy6 282 18 PDI PDI NNP cord-001249-awn9ayy6 282 19 and and CC cord-001249-awn9ayy6 282 20 ERp57 ERp57 NNS cord-001249-awn9ayy6 282 21 , , , cord-001249-awn9ayy6 282 22 in in IN cord-001249-awn9ayy6 282 23 infected infected JJ cord-001249-awn9ayy6 282 24 cells cell NNS cord-001249-awn9ayy6 282 25 , , , cord-001249-awn9ayy6 282 26 and and CC cord-001249-awn9ayy6 282 27 that that IN cord-001249-awn9ayy6 282 28 the the DT cord-001249-awn9ayy6 282 29 viral viral JJ cord-001249-awn9ayy6 282 30 PreGn pregn NN cord-001249-awn9ayy6 282 31 glycoprotein glycoprotein NN cord-001249-awn9ayy6 282 32 appears appear VBZ cord-001249-awn9ayy6 282 33 to to TO cord-001249-awn9ayy6 282 34 be be VB cord-001249-awn9ayy6 282 35 involved involve VBN cord-001249-awn9ayy6 282 36 in in IN cord-001249-awn9ayy6 282 37 this this DT cord-001249-awn9ayy6 282 38 process process NN cord-001249-awn9ayy6 282 39 . . . cord-001249-awn9ayy6 283 1 Redistribution redistribution NN cord-001249-awn9ayy6 283 2 of of IN cord-001249-awn9ayy6 283 3 PDI PDI NNP cord-001249-awn9ayy6 283 4 from from IN cord-001249-awn9ayy6 283 5 the the DT cord-001249-awn9ayy6 283 6 ER ER NNP cord-001249-awn9ayy6 283 7 of of IN cord-001249-awn9ayy6 283 8 NSDV NSDV NNP cord-001249-awn9ayy6 283 9 - - HYPH cord-001249-awn9ayy6 283 10 infected infect VBN cord-001249-awn9ayy6 283 11 cells cell NNS cord-001249-awn9ayy6 283 12 to to IN cord-001249-awn9ayy6 283 13 the the DT cord-001249-awn9ayy6 283 14 cell cell NN cord-001249-awn9ayy6 283 15 surface surface NN cord-001249-awn9ayy6 283 16 and/or and/or CC cord-001249-awn9ayy6 283 17 the the DT cord-001249-awn9ayy6 283 18 extracellular extracellular JJ cord-001249-awn9ayy6 283 19 environment environment NN cord-001249-awn9ayy6 283 20 may may MD cord-001249-awn9ayy6 283 21 play play VB cord-001249-awn9ayy6 283 22 a a DT cord-001249-awn9ayy6 283 23 crucial crucial JJ cord-001249-awn9ayy6 283 24 role role NN cord-001249-awn9ayy6 283 25 in in IN cord-001249-awn9ayy6 283 26 the the DT cord-001249-awn9ayy6 283 27 pathogenicity pathogenicity NN cord-001249-awn9ayy6 283 28 of of IN cord-001249-awn9ayy6 283 29 NSDV NSDV NNP cord-001249-awn9ayy6 283 30 . . . cord-001249-awn9ayy6 284 1 PDI PDI NNP cord-001249-awn9ayy6 284 2 may may MD cord-001249-awn9ayy6 284 3 enhance enhance VB cord-001249-awn9ayy6 284 4 virus virus NN cord-001249-awn9ayy6 284 5 infection infection NN cord-001249-awn9ayy6 284 6 or or CC cord-001249-awn9ayy6 284 7 may may MD cord-001249-awn9ayy6 284 8 contribute contribute VB cord-001249-awn9ayy6 284 9 to to IN cord-001249-awn9ayy6 284 10 the the DT cord-001249-awn9ayy6 284 11 observed observed JJ cord-001249-awn9ayy6 284 12 haemorrhagic haemorrhagic JJ cord-001249-awn9ayy6 284 13 outcome outcome NN cord-001249-awn9ayy6 284 14 of of IN cord-001249-awn9ayy6 284 15 the the DT cord-001249-awn9ayy6 284 16 infection infection NN cord-001249-awn9ayy6 284 17 , , , cord-001249-awn9ayy6 284 18 although although IN cord-001249-awn9ayy6 284 19 the the DT cord-001249-awn9ayy6 284 20 exact exact JJ cord-001249-awn9ayy6 284 21 importance importance NN cord-001249-awn9ayy6 284 22 of of IN cord-001249-awn9ayy6 284 23 extracellular extracellular JJ cord-001249-awn9ayy6 284 24 PDI PDI NNP cord-001249-awn9ayy6 284 25 and and CC cord-001249-awn9ayy6 284 26 ERp57 ERp57 NNS cord-001249-awn9ayy6 284 27 in in IN cord-001249-awn9ayy6 284 28 natural natural JJ cord-001249-awn9ayy6 284 29 infection infection NN cord-001249-awn9ayy6 284 30 remains remain VBZ cord-001249-awn9ayy6 284 31 to to TO cord-001249-awn9ayy6 284 32 be be VB cord-001249-awn9ayy6 284 33 determined determine VBN cord-001249-awn9ayy6 284 34 . . . cord-001249-awn9ayy6 285 1 In in IN cord-001249-awn9ayy6 285 2 recent recent JJ cord-001249-awn9ayy6 285 3 years year NNS cord-001249-awn9ayy6 285 4 , , , cord-001249-awn9ayy6 285 5 PDI PDI NNP cord-001249-awn9ayy6 285 6 has have VBZ cord-001249-awn9ayy6 285 7 become become VBN cord-001249-awn9ayy6 285 8 a a DT cord-001249-awn9ayy6 285 9 popular popular JJ cord-001249-awn9ayy6 285 10 potential potential JJ cord-001249-awn9ayy6 285 11 drug drug NN cord-001249-awn9ayy6 285 12 target target NN cord-001249-awn9ayy6 285 13 for for IN cord-001249-awn9ayy6 285 14 treatment treatment NN cord-001249-awn9ayy6 285 15 of of IN cord-001249-awn9ayy6 285 16 thrombosis thrombosis NN cord-001249-awn9ayy6 285 17 , , , cord-001249-awn9ayy6 285 18 neurodegenerative neurodegenerative JJ cord-001249-awn9ayy6 285 19 diseases disease NNS cord-001249-awn9ayy6 285 20 and and CC cord-001249-awn9ayy6 285 21 HIV HIV NNP cord-001249-awn9ayy6 285 22 infection infection NN cord-001249-awn9ayy6 285 23 [ [ -LRB- cord-001249-awn9ayy6 285 24 69 69 CD cord-001249-awn9ayy6 285 25 , , , cord-001249-awn9ayy6 285 26 121 121 CD cord-001249-awn9ayy6 285 27 , , , cord-001249-awn9ayy6 285 28 122 122 CD cord-001249-awn9ayy6 285 29 ] ] -RRB- cord-001249-awn9ayy6 285 30 and and CC cord-001249-awn9ayy6 285 31 it -PRON- PRP cord-001249-awn9ayy6 285 32 appears appear VBZ cord-001249-awn9ayy6 285 33 that that IN cord-001249-awn9ayy6 285 34 PDI PDI NNP cord-001249-awn9ayy6 285 35 could could MD cord-001249-awn9ayy6 285 36 also also RB cord-001249-awn9ayy6 285 37 act act VB cord-001249-awn9ayy6 285 38 as as IN cord-001249-awn9ayy6 285 39 a a DT cord-001249-awn9ayy6 285 40 target target NN cord-001249-awn9ayy6 285 41 for for IN cord-001249-awn9ayy6 285 42 treatment treatment NN cord-001249-awn9ayy6 285 43 of of IN cord-001249-awn9ayy6 285 44 at at IN cord-001249-awn9ayy6 285 45 least least JJS cord-001249-awn9ayy6 285 46 some some DT cord-001249-awn9ayy6 285 47 viral viral JJ cord-001249-awn9ayy6 285 48 haemorrhagic haemorrhagic JJ cord-001249-awn9ayy6 285 49 fevers fever NNS cord-001249-awn9ayy6 285 50 [ [ -LRB- cord-001249-awn9ayy6 285 51 86 86 CD cord-001249-awn9ayy6 285 52 ] ] -RRB- cord-001249-awn9ayy6 285 53 . . . cord-001249-awn9ayy6 286 1 While while IN cord-001249-awn9ayy6 286 2 a a DT cord-001249-awn9ayy6 286 3 wide wide JJ cord-001249-awn9ayy6 286 4 range range NN cord-001249-awn9ayy6 286 5 of of IN cord-001249-awn9ayy6 286 6 applications application NNS cord-001249-awn9ayy6 286 7 makes make VBZ cord-001249-awn9ayy6 286 8 PDI PDI NNP cord-001249-awn9ayy6 286 9 inhibitors inhibitor NNS cord-001249-awn9ayy6 286 10 attractive attractive JJ cord-001249-awn9ayy6 286 11 agents agent NNS cord-001249-awn9ayy6 286 12 , , , cord-001249-awn9ayy6 286 13 extracellular extracellular JJ cord-001249-awn9ayy6 286 14 PDI PDI NNP cord-001249-awn9ayy6 286 15 appears appear VBZ cord-001249-awn9ayy6 286 16 to to TO cord-001249-awn9ayy6 286 17 take take VB cord-001249-awn9ayy6 286 18 part part NN cord-001249-awn9ayy6 286 19 in in IN cord-001249-awn9ayy6 286 20 a a DT cord-001249-awn9ayy6 286 21 broad broad JJ cord-001249-awn9ayy6 286 22 range range NN cord-001249-awn9ayy6 286 23 of of IN cord-001249-awn9ayy6 286 24 physiological physiological JJ cord-001249-awn9ayy6 286 25 functions function NNS cord-001249-awn9ayy6 286 26 , , , cord-001249-awn9ayy6 286 27 and and CC cord-001249-awn9ayy6 286 28 therefore therefore RB cord-001249-awn9ayy6 286 29 drug drug NN cord-001249-awn9ayy6 286 30 safety safety NN cord-001249-awn9ayy6 286 31 might may MD cord-001249-awn9ayy6 286 32 become become VB cord-001249-awn9ayy6 286 33 a a DT cord-001249-awn9ayy6 286 34 limiting limit VBG cord-001249-awn9ayy6 286 35 factor factor NN cord-001249-awn9ayy6 286 36 for for IN cord-001249-awn9ayy6 286 37 the the DT cord-001249-awn9ayy6 286 38 development development NN cord-001249-awn9ayy6 286 39 of of IN cord-001249-awn9ayy6 286 40 drugs drug NNS cord-001249-awn9ayy6 286 41 which which WDT cord-001249-awn9ayy6 286 42 interfere interfere VBP cord-001249-awn9ayy6 286 43 with with IN cord-001249-awn9ayy6 286 44 enzymatic enzymatic JJ cord-001249-awn9ayy6 286 45 activity activity NN cord-001249-awn9ayy6 286 46 of of IN cord-001249-awn9ayy6 286 47 extracellular extracellular JJ cord-001249-awn9ayy6 286 48 PDI PDI NNP cord-001249-awn9ayy6 286 49 . . . cord-001249-awn9ayy6 287 1 Figure figure NN cord-001249-awn9ayy6 287 2 S1 S1 NNP cord-001249-awn9ayy6 287 3 Effect Effect NNP cord-001249-awn9ayy6 287 4 of of IN cord-001249-awn9ayy6 287 5 NSDV NSDV NNP cord-001249-awn9ayy6 287 6 infection infection NN cord-001249-awn9ayy6 287 7 on on IN cord-001249-awn9ayy6 287 8 the the DT cord-001249-awn9ayy6 287 9 cellular cellular JJ cord-001249-awn9ayy6 287 10 cytoskeleton cytoskeleton NN cord-001249-awn9ayy6 287 11 . . . cord-001249-awn9ayy6 288 1 Vero Vero NNP cord-001249-awn9ayy6 288 2 cells cell NNS cord-001249-awn9ayy6 288 3 were be VBD cord-001249-awn9ayy6 288 4 infected infect VBN cord-001249-awn9ayy6 288 5 with with IN cord-001249-awn9ayy6 288 6 NSDVi NSDVi NNS cord-001249-awn9ayy6 288 7 at at IN cord-001249-awn9ayy6 288 8 a a DT cord-001249-awn9ayy6 288 9 MOI MOI NNP cord-001249-awn9ayy6 288 10 of of IN cord-001249-awn9ayy6 288 11 0.3 0.3 CD cord-001249-awn9ayy6 288 12 . . . cord-001249-awn9ayy6 289 1 After after IN cord-001249-awn9ayy6 289 2 16 16 CD cord-001249-awn9ayy6 289 3 h h NN cord-001249-awn9ayy6 289 4 , , , cord-001249-awn9ayy6 289 5 cells cell NNS cord-001249-awn9ayy6 289 6 were be VBD cord-001249-awn9ayy6 289 7 fixed fix VBN cord-001249-awn9ayy6 289 8 using use VBG cord-001249-awn9ayy6 289 9 3 3 CD cord-001249-awn9ayy6 289 10 % % NN cord-001249-awn9ayy6 289 11 PFA PFA NNP cord-001249-awn9ayy6 289 12 and and CC cord-001249-awn9ayy6 289 13 ( ( -LRB- cord-001249-awn9ayy6 289 14 a a LS cord-001249-awn9ayy6 289 15 - - HYPH cord-001249-awn9ayy6 289 16 f f NN cord-001249-awn9ayy6 289 17 ) ) -RRB- cord-001249-awn9ayy6 289 18 stained stain VBN cord-001249-awn9ayy6 289 19 using use VBG cord-001249-awn9ayy6 289 20 Alexa Alexa NNP cord-001249-awn9ayy6 289 21 Fluor Fluor NNP cord-001249-awn9ayy6 289 22 488 488 CD cord-001249-awn9ayy6 289 23 Phalloidin Phalloidin NNP cord-001249-awn9ayy6 289 24 ( ( -LRB- cord-001249-awn9ayy6 289 25 green green JJ cord-001249-awn9ayy6 289 26 ; ; : cord-001249-awn9ayy6 289 27 actin actin JJ cord-001249-awn9ayy6 289 28 ) ) -RRB- cord-001249-awn9ayy6 289 29 and and CC cord-001249-awn9ayy6 289 30 rabbit rabbit NN cord-001249-awn9ayy6 289 31 anti anti NNP cord-001249-awn9ayy6 289 32 - - NNP cord-001249-awn9ayy6 289 33 N N NNP cord-001249-awn9ayy6 289 34 , , , cord-001249-awn9ayy6 289 35 followed follow VBN cord-001249-awn9ayy6 289 36 by by IN cord-001249-awn9ayy6 289 37 Alexa Alexa NNP cord-001249-awn9ayy6 289 38 Fluor Fluor NNP cord-001249-awn9ayy6 289 39 568 568 CD cord-001249-awn9ayy6 289 40 goat goat NN cord-001249-awn9ayy6 289 41 anti anti JJ cord-001249-awn9ayy6 289 42 - - JJ cord-001249-awn9ayy6 289 43 rabbit rabbit JJ cord-001249-awn9ayy6 289 44 IgG igg NN cord-001249-awn9ayy6 289 45 ( ( -LRB- cord-001249-awn9ayy6 289 46 red red NNP cord-001249-awn9ayy6 289 47 ) ) -RRB- cord-001249-awn9ayy6 289 48 ; ; : cord-001249-awn9ayy6 289 49 ( ( -LRB- cord-001249-awn9ayy6 289 50 g g NN cord-001249-awn9ayy6 289 51 - - HYPH cord-001249-awn9ayy6 289 52 o o NNP cord-001249-awn9ayy6 289 53 ) ) -RRB- cord-001249-awn9ayy6 289 54 cells cell NNS cord-001249-awn9ayy6 289 55 were be VBD cord-001249-awn9ayy6 289 56 opened open VBN cord-001249-awn9ayy6 289 57 with with IN cord-001249-awn9ayy6 289 58 ice ice NN cord-001249-awn9ayy6 289 59 - - HYPH cord-001249-awn9ayy6 289 60 cold cold JJ cord-001249-awn9ayy6 289 61 methanol methanol NN cord-001249-awn9ayy6 289 62 and and CC cord-001249-awn9ayy6 289 63 stained stain VBN cord-001249-awn9ayy6 289 64 using use VBG cord-001249-awn9ayy6 289 65 mouse mouse NN cord-001249-awn9ayy6 289 66 antia antia NN cord-001249-awn9ayy6 289 67 - - HYPH cord-001249-awn9ayy6 289 68 tubulin tubulin NN cord-001249-awn9ayy6 289 69 antibody antibody NN cord-001249-awn9ayy6 289 70 and and CC cord-001249-awn9ayy6 289 71 rabbit rabbit NN cord-001249-awn9ayy6 289 72 anti anti NNP cord-001249-awn9ayy6 289 73 - - NNP cord-001249-awn9ayy6 289 74 N N NNP cord-001249-awn9ayy6 289 75 , , , cord-001249-awn9ayy6 289 76 followed follow VBN cord-001249-awn9ayy6 289 77 by by IN cord-001249-awn9ayy6 289 78 Alexa Alexa NNP cord-001249-awn9ayy6 289 79 Fluor Fluor NNP cord-001249-awn9ayy6 289 80 488 488 CD cord-001249-awn9ayy6 289 81 goat goat NN cord-001249-awn9ayy6 289 82 anti anti JJ cord-001249-awn9ayy6 289 83 - - JJ cord-001249-awn9ayy6 289 84 mouse mouse JJ cord-001249-awn9ayy6 289 85 IgG IgG NNP cord-001249-awn9ayy6 289 86 ( ( -LRB- cord-001249-awn9ayy6 289 87 green green NNP cord-001249-awn9ayy6 289 88 ) ) -RRB- cord-001249-awn9ayy6 289 89 and and CC cord-001249-awn9ayy6 289 90 Alexa Alexa NNP cord-001249-awn9ayy6 289 91 Fluor Fluor NNP cord-001249-awn9ayy6 289 92 568 568 CD cord-001249-awn9ayy6 289 93 goat goat NN cord-001249-awn9ayy6 289 94 anti anti JJ cord-001249-awn9ayy6 289 95 - - JJ cord-001249-awn9ayy6 289 96 rabbit rabbit JJ cord-001249-awn9ayy6 289 97 IgG igg NN cord-001249-awn9ayy6 289 98 ( ( -LRB- cord-001249-awn9ayy6 289 99 red red NNP cord-001249-awn9ayy6 289 100 ) ) -RRB- cord-001249-awn9ayy6 289 101 . . . cord-001249-awn9ayy6 290 1 Nuclei nucleus NNS cord-001249-awn9ayy6 290 2 were be VBD cord-001249-awn9ayy6 290 3 counterstained counterstaine VBN cord-001249-awn9ayy6 290 4 using use VBG cord-001249-awn9ayy6 290 5 DAPI DAPI NNP cord-001249-awn9ayy6 290 6 ( ( -LRB- cord-001249-awn9ayy6 290 7 blue blue JJ cord-001249-awn9ayy6 290 8 ) ) -RRB- cord-001249-awn9ayy6 290 9 . . . cord-001249-awn9ayy6 291 1 Dashed dash VBN cord-001249-awn9ayy6 291 2 boxes box NNS cord-001249-awn9ayy6 291 3 in in IN cord-001249-awn9ayy6 291 4 a a DT cord-001249-awn9ayy6 291 5 - - HYPH cord-001249-awn9ayy6 291 6 c c NN cord-001249-awn9ayy6 291 7 and and CC cord-001249-awn9ayy6 291 8 g g NN cord-001249-awn9ayy6 292 1 -i -i NFP cord-001249-awn9ayy6 292 2 indicate indicate VBP cord-001249-awn9ayy6 292 3 the the DT cord-001249-awn9ayy6 292 4 source source NN cord-001249-awn9ayy6 292 5 of of IN cord-001249-awn9ayy6 292 6 the the DT cord-001249-awn9ayy6 292 7 enlarged enlarged JJ cord-001249-awn9ayy6 292 8 areas area NNS cord-001249-awn9ayy6 292 9 shown show VBN cord-001249-awn9ayy6 292 10 in in IN cord-001249-awn9ayy6 292 11 d d NNP cord-001249-awn9ayy6 292 12 - - HYPH cord-001249-awn9ayy6 292 13 f f NNP cord-001249-awn9ayy6 292 14 and and CC cord-001249-awn9ayy6 292 15 j j NNP cord-001249-awn9ayy6 292 16 - - HYPH cord-001249-awn9ayy6 292 17 l l NNP cord-001249-awn9ayy6 292 18 , , , cord-001249-awn9ayy6 292 19 respectively respectively RB cord-001249-awn9ayy6 292 20 . . . cord-001249-awn9ayy6 293 1 ( ( -LRB- cord-001249-awn9ayy6 293 2 TIF tif NN cord-001249-awn9ayy6 293 3 ) ) -RRB- cord-001249-awn9ayy6 294 1 Figure figure NN cord-001249-awn9ayy6 294 2 S2 S2 NNP cord-001249-awn9ayy6 294 3 Effect Effect NNP cord-001249-awn9ayy6 294 4 of of IN cord-001249-awn9ayy6 294 5 NSDV NSDV NNP cord-001249-awn9ayy6 294 6 infection infection NN cord-001249-awn9ayy6 294 7 on on IN cord-001249-awn9ayy6 294 8 PDI PDI NNP cord-001249-awn9ayy6 294 9 in in IN cord-001249-awn9ayy6 294 10 human human JJ cord-001249-awn9ayy6 294 11 and and CC cord-001249-awn9ayy6 294 12 bovine bovine JJ cord-001249-awn9ayy6 294 13 cell cell NN cord-001249-awn9ayy6 294 14 lines line NNS cord-001249-awn9ayy6 294 15 . . . cord-001249-awn9ayy6 295 1 A549 a549 JJ cord-001249-awn9ayy6 295 2 ( ( -LRB- cord-001249-awn9ayy6 295 3 human human JJ cord-001249-awn9ayy6 295 4 lung lung NN cord-001249-awn9ayy6 295 5 ) ) -RRB- cord-001249-awn9ayy6 295 6 cells cell NNS cord-001249-awn9ayy6 295 7 ( ( -LRB- cord-001249-awn9ayy6 295 8 a a NNP cord-001249-awn9ayy6 295 9 - - HYPH cord-001249-awn9ayy6 295 10 c c NN cord-001249-awn9ayy6 295 11 ) ) -RRB- cord-001249-awn9ayy6 295 12 or or CC cord-001249-awn9ayy6 295 13 BFA BFA NNP cord-001249-awn9ayy6 295 14 ( ( -LRB- cord-001249-awn9ayy6 295 15 bovine bovine JJ cord-001249-awn9ayy6 295 16 foetal foetal JJ cord-001249-awn9ayy6 295 17 aortic aortic JJ cord-001249-awn9ayy6 295 18 endothelial endothelial NN cord-001249-awn9ayy6 295 19 ) ) -RRB- cord-001249-awn9ayy6 295 20 cells cell NNS cord-001249-awn9ayy6 295 21 ( ( -LRB- cord-001249-awn9ayy6 295 22 d d NN cord-001249-awn9ayy6 295 23 - - : cord-001249-awn9ayy6 295 24 i i NNP cord-001249-awn9ayy6 295 25 ) ) -RRB- cord-001249-awn9ayy6 295 26 were be VBD cord-001249-awn9ayy6 295 27 infected infect VBN cord-001249-awn9ayy6 295 28 with with IN cord-001249-awn9ayy6 295 29 NSDVi NSDVi NNS cord-001249-awn9ayy6 295 30 at at IN cord-001249-awn9ayy6 295 31 a a DT cord-001249-awn9ayy6 295 32 MOI MOI NNP cord-001249-awn9ayy6 295 33 of of IN cord-001249-awn9ayy6 295 34 0.3 0.3 CD cord-001249-awn9ayy6 295 35 . . . cord-001249-awn9ayy6 296 1 After after IN cord-001249-awn9ayy6 296 2 16 16 CD cord-001249-awn9ayy6 296 3 h h NN cord-001249-awn9ayy6 296 4 ( ( -LRB- cord-001249-awn9ayy6 296 5 a a NNP cord-001249-awn9ayy6 296 6 - - HYPH cord-001249-awn9ayy6 296 7 c c NN cord-001249-awn9ayy6 296 8 ) ) -RRB- cord-001249-awn9ayy6 296 9 or or CC cord-001249-awn9ayy6 296 10 72 72 CD cord-001249-awn9ayy6 296 11 h h NN cord-001249-awn9ayy6 296 12 ( ( -LRB- cord-001249-awn9ayy6 296 13 d d NN cord-001249-awn9ayy6 296 14 - - HYPH cord-001249-awn9ayy6 296 15 f f NNP cord-001249-awn9ayy6 296 16 ) ) -RRB- cord-001249-awn9ayy6 296 17 cells cell NNS cord-001249-awn9ayy6 296 18 were be VBD cord-001249-awn9ayy6 296 19 fixed fix VBN cord-001249-awn9ayy6 296 20 and and CC cord-001249-awn9ayy6 296 21 stained stain VBN cord-001249-awn9ayy6 296 22 using use VBG cord-001249-awn9ayy6 296 23 specific specific JJ cord-001249-awn9ayy6 296 24 antibodies antibody NNS cord-001249-awn9ayy6 296 25 . . . cord-001249-awn9ayy6 297 1 ( ( -LRB- cord-001249-awn9ayy6 297 2 a a LS cord-001249-awn9ayy6 297 3 - - HYPH cord-001249-awn9ayy6 297 4 f f NN cord-001249-awn9ayy6 297 5 ) ) -RRB- cord-001249-awn9ayy6 297 6 Cells cell NNS cord-001249-awn9ayy6 297 7 were be VBD cord-001249-awn9ayy6 297 8 fixed fix VBN cord-001249-awn9ayy6 297 9 using use VBG cord-001249-awn9ayy6 297 10 3 3 CD cord-001249-awn9ayy6 297 11 % % NN cord-001249-awn9ayy6 298 1 PFA PFA NNP cord-001249-awn9ayy6 298 2 followed follow VBD cord-001249-awn9ayy6 298 3 by by IN cord-001249-awn9ayy6 298 4 ice ice NN cord-001249-awn9ayy6 298 5 - - HYPH cord-001249-awn9ayy6 298 6 cold cold JJ cord-001249-awn9ayy6 298 7 methanol methanol NN cord-001249-awn9ayy6 298 8 , , , cord-001249-awn9ayy6 298 9 and and CC cord-001249-awn9ayy6 298 10 then then RB cord-001249-awn9ayy6 298 11 stained stain VBD cord-001249-awn9ayy6 298 12 with with IN cord-001249-awn9ayy6 298 13 mouse mouse NN cord-001249-awn9ayy6 298 14 anti anti JJ cord-001249-awn9ayy6 298 15 - - NNP cord-001249-awn9ayy6 298 16 PDI pdi JJ cord-001249-awn9ayy6 298 17 ( ( -LRB- cord-001249-awn9ayy6 298 18 clone clone NN cord-001249-awn9ayy6 298 19 1D3 1d3 CD cord-001249-awn9ayy6 298 20 ) ) -RRB- cord-001249-awn9ayy6 298 21 and and CC cord-001249-awn9ayy6 298 22 rabbit rabbit NN cord-001249-awn9ayy6 298 23 anti anti NNP cord-001249-awn9ayy6 298 24 - - NNP cord-001249-awn9ayy6 298 25 N. n. NN cord-001249-awn9ayy6 298 26 ( ( -LRB- cord-001249-awn9ayy6 298 27 g g NN cord-001249-awn9ayy6 298 28 - - : cord-001249-awn9ayy6 298 29 i i NNP cord-001249-awn9ayy6 298 30 ) ) -RRB- cord-001249-awn9ayy6 298 31 Cells cell NNS cord-001249-awn9ayy6 298 32 fixed fix VBN cord-001249-awn9ayy6 298 33 with with IN cord-001249-awn9ayy6 298 34 3 3 CD cord-001249-awn9ayy6 298 35 % % NN cord-001249-awn9ayy6 298 36 PFA PFA NNP cord-001249-awn9ayy6 298 37 only only RB cord-001249-awn9ayy6 298 38 , , , cord-001249-awn9ayy6 298 39 labelled label VBN cord-001249-awn9ayy6 298 40 with with IN cord-001249-awn9ayy6 298 41 mouse mouse NN cord-001249-awn9ayy6 298 42 anti anti JJ cord-001249-awn9ayy6 298 43 - - NNP cord-001249-awn9ayy6 298 44 PDI pdi JJ cord-001249-awn9ayy6 298 45 ( ( -LRB- cord-001249-awn9ayy6 298 46 clone clone NN cord-001249-awn9ayy6 298 47 1D3 1d3 CD cord-001249-awn9ayy6 298 48 ) ) -RRB- cord-001249-awn9ayy6 298 49 , , , cord-001249-awn9ayy6 298 50 then then RB cord-001249-awn9ayy6 298 51 again again RB cord-001249-awn9ayy6 298 52 fixed fix VBN cord-001249-awn9ayy6 298 53 with with IN cord-001249-awn9ayy6 298 54 3 3 CD cord-001249-awn9ayy6 298 55 % % NN cord-001249-awn9ayy6 298 56 PFA PFA NNP cord-001249-awn9ayy6 298 57 , , , cord-001249-awn9ayy6 298 58 opened open VBD cord-001249-awn9ayy6 298 59 with with IN cord-001249-awn9ayy6 298 60 ice ice NN cord-001249-awn9ayy6 298 61 - - HYPH cord-001249-awn9ayy6 298 62 cold cold JJ cord-001249-awn9ayy6 298 63 methanol methanol NN cord-001249-awn9ayy6 298 64 and and CC cord-001249-awn9ayy6 298 65 stained stain VBN cord-001249-awn9ayy6 298 66 with with IN cord-001249-awn9ayy6 298 67 rabbit rabbit NN cord-001249-awn9ayy6 298 68 anti anti JJ cord-001249-awn9ayy6 298 69 - - NNP cord-001249-awn9ayy6 298 70 N. N. NNP cord-001249-awn9ayy6 298 71 Proteins protein NNS cord-001249-awn9ayy6 298 72 were be VBD cord-001249-awn9ayy6 298 73 visualised visualise VBN cord-001249-awn9ayy6 298 74 using use VBG cord-001249-awn9ayy6 298 75 Alexa Alexa NNP cord-001249-awn9ayy6 298 76 Fluor Fluor NNP cord-001249-awn9ayy6 298 77 488 488 CD cord-001249-awn9ayy6 298 78 goat goat NN cord-001249-awn9ayy6 298 79 anti anti JJ cord-001249-awn9ayy6 298 80 - - JJ cord-001249-awn9ayy6 298 81 mouse mouse JJ cord-001249-awn9ayy6 298 82 IgG IgG NNP cord-001249-awn9ayy6 298 83 ( ( -LRB- cord-001249-awn9ayy6 298 84 green green NNP cord-001249-awn9ayy6 298 85 ) ) -RRB- cord-001249-awn9ayy6 298 86 and and CC cord-001249-awn9ayy6 298 87 Alexa Alexa NNP cord-001249-awn9ayy6 298 88 Fluor Fluor NNP cord-001249-awn9ayy6 298 89 568 568 CD cord-001249-awn9ayy6 298 90 goat goat NN cord-001249-awn9ayy6 298 91 anti anti JJ cord-001249-awn9ayy6 298 92 - - JJ cord-001249-awn9ayy6 298 93 rabbit rabbit JJ cord-001249-awn9ayy6 298 94 IgG igg NN cord-001249-awn9ayy6 298 95 ( ( -LRB- cord-001249-awn9ayy6 298 96 red red NNP cord-001249-awn9ayy6 298 97 ) ) -RRB- cord-001249-awn9ayy6 298 98 . . . cord-001249-awn9ayy6 299 1 Nuclei nucleus NNS cord-001249-awn9ayy6 299 2 were be VBD cord-001249-awn9ayy6 299 3 counterstained counterstaine VBN cord-001249-awn9ayy6 299 4 using use VBG cord-001249-awn9ayy6 299 5 DAPI DAPI NNP cord-001249-awn9ayy6 299 6 ( ( -LRB- cord-001249-awn9ayy6 299 7 blue blue JJ cord-001249-awn9ayy6 299 8 ) ) -RRB- cord-001249-awn9ayy6 299 9 . . . cord-001249-awn9ayy6 300 1 Bars bar NNS cord-001249-awn9ayy6 300 2 correspond correspond VBP cord-001249-awn9ayy6 300 3 to to IN cord-001249-awn9ayy6 300 4 40 40 CD cord-001249-awn9ayy6 300 5 mm mm NNS cord-001249-awn9ayy6 300 6 . . . cord-001249-awn9ayy6 301 1 ( ( -LRB- cord-001249-awn9ayy6 301 2 TIF tif NN cord-001249-awn9ayy6 301 3 ) ) -RRB- cord-001249-awn9ayy6 302 1 Figure figure NN cord-001249-awn9ayy6 302 2 S3 S3 NNP cord-001249-awn9ayy6 302 3 Time Time NNP cord-001249-awn9ayy6 302 4 course course NN cord-001249-awn9ayy6 302 5 of of IN cord-001249-awn9ayy6 302 6 changes change NNS cord-001249-awn9ayy6 302 7 to to IN cord-001249-awn9ayy6 302 8 ERp57 ERp57 NNS cord-001249-awn9ayy6 302 9 in in IN cord-001249-awn9ayy6 302 10 NSDVinfected nsdvinfected JJ cord-001249-awn9ayy6 302 11 cells cell NNS cord-001249-awn9ayy6 302 12 . . . cord-001249-awn9ayy6 303 1 Vero Vero NNP cord-001249-awn9ayy6 303 2 cells cell NNS cord-001249-awn9ayy6 303 3 were be VBD cord-001249-awn9ayy6 303 4 infected infect VBN cord-001249-awn9ayy6 303 5 with with IN cord-001249-awn9ayy6 303 6 NSDVi NSDVi NNS cord-001249-awn9ayy6 303 7 at at IN cord-001249-awn9ayy6 303 8 a a DT cord-001249-awn9ayy6 303 9 MOI MOI NNP cord-001249-awn9ayy6 303 10 of of IN cord-001249-awn9ayy6 303 11 6 6 CD cord-001249-awn9ayy6 303 12 and and CC cord-001249-awn9ayy6 303 13 fixed fix VBN cord-001249-awn9ayy6 303 14 at at IN cord-001249-awn9ayy6 303 15 8 8 CD cord-001249-awn9ayy6 303 16 , , , cord-001249-awn9ayy6 303 17 12 12 CD cord-001249-awn9ayy6 303 18 and and CC cord-001249-awn9ayy6 303 19 16 16 CD cord-001249-awn9ayy6 303 20 hpi hpi NNP cord-001249-awn9ayy6 303 21 . . . cord-001249-awn9ayy6 304 1 ( ( -LRB- cord-001249-awn9ayy6 304 2 a a LS cord-001249-awn9ayy6 304 3 - - : cord-001249-awn9ayy6 304 4 i i NN cord-001249-awn9ayy6 304 5 ) ) -RRB- cord-001249-awn9ayy6 304 6 Cells cell NNS cord-001249-awn9ayy6 304 7 were be VBD cord-001249-awn9ayy6 304 8 fixed fix VBN cord-001249-awn9ayy6 304 9 using use VBG cord-001249-awn9ayy6 304 10 3 3 CD cord-001249-awn9ayy6 304 11 % % NN cord-001249-awn9ayy6 305 1 PFA PFA NNP cord-001249-awn9ayy6 305 2 followed follow VBD cord-001249-awn9ayy6 305 3 by by IN cord-001249-awn9ayy6 305 4 ice ice NN cord-001249-awn9ayy6 305 5 cold cold JJ cord-001249-awn9ayy6 305 6 methanol methanol NN cord-001249-awn9ayy6 305 7 and and CC cord-001249-awn9ayy6 305 8 stained stain VBN cord-001249-awn9ayy6 305 9 with with IN cord-001249-awn9ayy6 305 10 rabbit rabbit NN cord-001249-awn9ayy6 305 11 anti anti JJ cord-001249-awn9ayy6 305 12 - - JJ cord-001249-awn9ayy6 305 13 ERp57 erp57 JJ cord-001249-awn9ayy6 305 14 antibody antibody NN cord-001249-awn9ayy6 305 15 and and CC cord-001249-awn9ayy6 305 16 mouse mouse NN cord-001249-awn9ayy6 305 17 anti anti NNP cord-001249-awn9ayy6 305 18 - - NNP cord-001249-awn9ayy6 305 19 PreGn PreGn NNP cord-001249-awn9ayy6 305 20 . . . cord-001249-awn9ayy6 306 1 ( ( -LRB- cord-001249-awn9ayy6 306 2 j j NNP cord-001249-awn9ayy6 306 3 - - HYPH cord-001249-awn9ayy6 306 4 l l NNP cord-001249-awn9ayy6 306 5 ) ) -RRB- cord-001249-awn9ayy6 306 6 cells cell NNS cord-001249-awn9ayy6 306 7 were be VBD cord-001249-awn9ayy6 306 8 fixed fix VBN cord-001249-awn9ayy6 306 9 with with IN cord-001249-awn9ayy6 306 10 3 3 CD cord-001249-awn9ayy6 306 11 % % NN cord-001249-awn9ayy6 306 12 PFA PFA NNP cord-001249-awn9ayy6 306 13 , , , cord-001249-awn9ayy6 306 14 labelled label VBN cord-001249-awn9ayy6 306 15 with with IN cord-001249-awn9ayy6 306 16 rabbit rabbit NN cord-001249-awn9ayy6 306 17 anti anti JJ cord-001249-awn9ayy6 306 18 - - JJ cord-001249-awn9ayy6 306 19 ERp57 erp57 JJ cord-001249-awn9ayy6 306 20 antibody antibody NN cord-001249-awn9ayy6 306 21 , , , cord-001249-awn9ayy6 306 22 again again RB cord-001249-awn9ayy6 306 23 fixed fix VBN cord-001249-awn9ayy6 306 24 with with IN cord-001249-awn9ayy6 306 25 3 3 CD cord-001249-awn9ayy6 306 26 % % NN cord-001249-awn9ayy6 306 27 PFA PFA NNP cord-001249-awn9ayy6 306 28 , , , cord-001249-awn9ayy6 306 29 opened open VBD cord-001249-awn9ayy6 306 30 with with IN cord-001249-awn9ayy6 306 31 ice ice NN cord-001249-awn9ayy6 306 32 - - HYPH cord-001249-awn9ayy6 306 33 cold cold JJ cord-001249-awn9ayy6 306 34 methanol methanol NN cord-001249-awn9ayy6 306 35 , , , cord-001249-awn9ayy6 306 36 and and CC cord-001249-awn9ayy6 306 37 labelled label VBN cord-001249-awn9ayy6 306 38 using use VBG cord-001249-awn9ayy6 306 39 mouse mouse NN cord-001249-awn9ayy6 306 40 anti anti NNP cord-001249-awn9ayy6 306 41 - - NNP cord-001249-awn9ayy6 306 42 PreGn PreGn NNP cord-001249-awn9ayy6 306 43 . . . cord-001249-awn9ayy6 307 1 Proteins protein NNS cord-001249-awn9ayy6 307 2 were be VBD cord-001249-awn9ayy6 307 3 visualised visualise VBN cord-001249-awn9ayy6 307 4 with with IN cord-001249-awn9ayy6 307 5 Alexa Alexa NNP cord-001249-awn9ayy6 307 6 Fluor Fluor NNP cord-001249-awn9ayy6 307 7 488 488 CD cord-001249-awn9ayy6 307 8 goat goat NN cord-001249-awn9ayy6 307 9 anti anti JJ cord-001249-awn9ayy6 307 10 - - JJ cord-001249-awn9ayy6 307 11 mouse mouse JJ cord-001249-awn9ayy6 307 12 IgG IgG NNP cord-001249-awn9ayy6 307 13 ( ( -LRB- cord-001249-awn9ayy6 307 14 green green NNP cord-001249-awn9ayy6 307 15 ) ) -RRB- cord-001249-awn9ayy6 307 16 and and CC cord-001249-awn9ayy6 307 17 Alexa Alexa NNP cord-001249-awn9ayy6 307 18 Fluor Fluor NNP cord-001249-awn9ayy6 307 19 568 568 CD cord-001249-awn9ayy6 307 20 goat goat NN cord-001249-awn9ayy6 307 21 anti anti JJ cord-001249-awn9ayy6 307 22 - - JJ cord-001249-awn9ayy6 307 23 rabbit rabbit JJ cord-001249-awn9ayy6 307 24 IgG igg NN cord-001249-awn9ayy6 307 25 ( ( -LRB- cord-001249-awn9ayy6 307 26 red red NNP cord-001249-awn9ayy6 307 27 ) ) -RRB- cord-001249-awn9ayy6 307 28 . . . cord-001249-awn9ayy6 308 1 Nuclei nucleus NNS cord-001249-awn9ayy6 308 2 were be VBD cord-001249-awn9ayy6 308 3 counterstained counterstaine VBN cord-001249-awn9ayy6 308 4 using use VBG cord-001249-awn9ayy6 308 5 DAPI DAPI NNP cord-001249-awn9ayy6 308 6 ( ( -LRB- cord-001249-awn9ayy6 308 7 blue blue JJ cord-001249-awn9ayy6 308 8 ) ) -RRB- cord-001249-awn9ayy6 308 9 . . . cord-001249-awn9ayy6 309 1 Bars bar NNS cord-001249-awn9ayy6 309 2 correspond correspond VBP cord-001249-awn9ayy6 309 3 to to IN cord-001249-awn9ayy6 309 4 16 16 CD cord-001249-awn9ayy6 309 5 mm mm NN cord-001249-awn9ayy6 309 6 . . . cord-001249-awn9ayy6 310 1 ( ( -LRB- cord-001249-awn9ayy6 310 2 TIF tif NN cord-001249-awn9ayy6 310 3 ) ) -RRB- cord-001249-awn9ayy6 311 1 Figure figure NN cord-001249-awn9ayy6 311 2 S4 S4 NNP cord-001249-awn9ayy6 311 3 Differences difference NNS cord-001249-awn9ayy6 311 4 in in IN cord-001249-awn9ayy6 311 5 the the DT cord-001249-awn9ayy6 311 6 expression expression NN cord-001249-awn9ayy6 311 7 levels level NNS cord-001249-awn9ayy6 311 8 of of IN cord-001249-awn9ayy6 311 9 N N NNP cord-001249-awn9ayy6 311 10 and and CC cord-001249-awn9ayy6 311 11 PreGn pregn PRP cord-001249-awn9ayy6 311 12 in in IN cord-001249-awn9ayy6 311 13 NSDV NSDV NNP cord-001249-awn9ayy6 311 14 - - HYPH cord-001249-awn9ayy6 311 15 infected infect VBN cord-001249-awn9ayy6 311 16 cells cell NNS cord-001249-awn9ayy6 311 17 . . . cord-001249-awn9ayy6 312 1 Samples sample NNS cord-001249-awn9ayy6 312 2 were be VBD cord-001249-awn9ayy6 312 3 prepared prepare VBN cord-001249-awn9ayy6 312 4 as as IN cord-001249-awn9ayy6 312 5 for for IN cord-001249-awn9ayy6 312 6 Figure figure NN cord-001249-awn9ayy6 312 7 1 1 CD cord-001249-awn9ayy6 312 8 except except IN cord-001249-awn9ayy6 312 9 that that IN cord-001249-awn9ayy6 312 10 cells cell NNS cord-001249-awn9ayy6 312 11 were be VBD cord-001249-awn9ayy6 312 12 stained stain VBN cord-001249-awn9ayy6 312 13 with with IN cord-001249-awn9ayy6 312 14 rabbit rabbit NN cord-001249-awn9ayy6 312 15 anti anti JJ cord-001249-awn9ayy6 312 16 - - NNP cord-001249-awn9ayy6 312 17 N n JJ cord-001249-awn9ayy6 312 18 protein protein NN cord-001249-awn9ayy6 312 19 and and CC cord-001249-awn9ayy6 312 20 mouse mouse NN cord-001249-awn9ayy6 312 21 anti anti NNP cord-001249-awn9ayy6 312 22 - - JJ cord-001249-awn9ayy6 312 23 PreGn pregn JJ cord-001249-awn9ayy6 312 24 antibody antibody NN cord-001249-awn9ayy6 312 25 . . . cord-001249-awn9ayy6 313 1 Proteins protein NNS cord-001249-awn9ayy6 313 2 were be VBD cord-001249-awn9ayy6 313 3 visualised visualise VBN cord-001249-awn9ayy6 313 4 using use VBG cord-001249-awn9ayy6 313 5 Alexa Alexa NNP cord-001249-awn9ayy6 313 6 Fluor Fluor NNP cord-001249-awn9ayy6 313 7 488 488 CD cord-001249-awn9ayy6 313 8 goat goat NN cord-001249-awn9ayy6 313 9 anti anti JJ cord-001249-awn9ayy6 313 10 - - JJ cord-001249-awn9ayy6 313 11 mouse mouse JJ cord-001249-awn9ayy6 313 12 IgG IgG NNP cord-001249-awn9ayy6 313 13 ( ( -LRB- cord-001249-awn9ayy6 313 14 green green NNP cord-001249-awn9ayy6 313 15 ) ) -RRB- cord-001249-awn9ayy6 313 16 and and CC cord-001249-awn9ayy6 313 17 Alexa Alexa NNP cord-001249-awn9ayy6 313 18 Fluor Fluor NNP cord-001249-awn9ayy6 313 19 568 568 CD cord-001249-awn9ayy6 313 20 goat goat NN cord-001249-awn9ayy6 313 21 anti anti JJ cord-001249-awn9ayy6 313 22 - - JJ cord-001249-awn9ayy6 313 23 rabbit rabbit JJ cord-001249-awn9ayy6 313 24 IgG igg NN cord-001249-awn9ayy6 313 25 ( ( -LRB- cord-001249-awn9ayy6 313 26 red red NNP cord-001249-awn9ayy6 313 27 ) ) -RRB- cord-001249-awn9ayy6 313 28 . . . cord-001249-awn9ayy6 314 1 Nuclei nucleus NNS cord-001249-awn9ayy6 314 2 were be VBD cord-001249-awn9ayy6 314 3 counterstained counterstaine VBN cord-001249-awn9ayy6 314 4 using use VBG cord-001249-awn9ayy6 314 5 DAPI DAPI NNP cord-001249-awn9ayy6 314 6 ( ( -LRB- cord-001249-awn9ayy6 314 7 blue blue JJ cord-001249-awn9ayy6 314 8 ) ) -RRB- cord-001249-awn9ayy6 314 9 . . . cord-001249-awn9ayy6 315 1 Bars bar NNS cord-001249-awn9ayy6 315 2 correspond correspond VBP cord-001249-awn9ayy6 315 3 to to IN cord-001249-awn9ayy6 315 4 40 40 CD cord-001249-awn9ayy6 315 5 mm mm NNS cord-001249-awn9ayy6 315 6 . . . cord-001249-awn9ayy6 316 1 Arrows arrow NNS cord-001249-awn9ayy6 316 2 indicate indicate VBP cord-001249-awn9ayy6 316 3 cells cell NNS cord-001249-awn9ayy6 316 4 where where WRB cord-001249-awn9ayy6 316 5 N n CD cord-001249-awn9ayy6 316 6 but but CC cord-001249-awn9ayy6 316 7 not not RB cord-001249-awn9ayy6 317 1 PreGn pregn PRP cord-001249-awn9ayy6 317 2 was be VBD cord-001249-awn9ayy6 317 3 detected detect VBN cord-001249-awn9ayy6 317 4 . . . cord-001249-awn9ayy6 318 1 ( ( -LRB- cord-001249-awn9ayy6 318 2 TIF tif NN cord-001249-awn9ayy6 318 3 ) ) -RRB- cord-001249-awn9ayy6 319 1 Figure figure NN cord-001249-awn9ayy6 319 2 S5 S5 NNP cord-001249-awn9ayy6 319 3 Localisation Localisation NNP cord-001249-awn9ayy6 319 4 of of IN cord-001249-awn9ayy6 319 5 plasmid plasmid NN cord-001249-awn9ayy6 319 6 - - HYPH cord-001249-awn9ayy6 319 7 expressed express VBN cord-001249-awn9ayy6 319 8 NSDV NSDV NNP cord-001249-awn9ayy6 319 9 glycoproteins glycoprotein NNS cord-001249-awn9ayy6 319 10 . . . cord-001249-awn9ayy6 320 1 Vero Vero NNP cord-001249-awn9ayy6 320 2 were be VBD cord-001249-awn9ayy6 320 3 transfected transfecte VBN cord-001249-awn9ayy6 320 4 with with IN cord-001249-awn9ayy6 320 5 1 1 CD cord-001249-awn9ayy6 320 6 mg mg NN cord-001249-awn9ayy6 320 7 of of IN cord-001249-awn9ayy6 320 8 pCAGGs_MCSII_PreGn_V5 pcaggs_mcsii_pregn_v5 NN cord-001249-awn9ayy6 320 9 ( ( -LRB- cord-001249-awn9ayy6 320 10 a a FW cord-001249-awn9ayy6 320 11 - - HYPH cord-001249-awn9ayy6 320 12 c c NN cord-001249-awn9ayy6 320 13 ) ) -RRB- cord-001249-awn9ayy6 320 14 , , , cord-001249-awn9ayy6 320 15 pCAGGs_MCSII_PreGc_V5 pCAGGs_MCSII_PreGc_V5 NNP cord-001249-awn9ayy6 320 16 ( ( -LRB- cord-001249-awn9ayy6 320 17 d d NNP cord-001249-awn9ayy6 320 18 - - HYPH cord-001249-awn9ayy6 320 19 f f NNP cord-001249-awn9ayy6 320 20 ) ) -RRB- cord-001249-awn9ayy6 320 21 or or CC cord-001249-awn9ayy6 320 22 pCAGGs_MCSII_NSM_V5 pCAGGs_MCSII_NSM_V5 NNP cord-001249-awn9ayy6 320 23 ( ( -LRB- cord-001249-awn9ayy6 320 24 g g NN cord-001249-awn9ayy6 320 25 - - HYPH cord-001249-awn9ayy6 320 26 i i NNP cord-001249-awn9ayy6 320 27 ) ) -RRB- cord-001249-awn9ayy6 320 28 . . . cord-001249-awn9ayy6 321 1 After after IN cord-001249-awn9ayy6 321 2 24 24 CD cord-001249-awn9ayy6 321 3 h h NN cord-001249-awn9ayy6 321 4 , , , cord-001249-awn9ayy6 321 5 cells cell NNS cord-001249-awn9ayy6 321 6 were be VBD cord-001249-awn9ayy6 321 7 fixed fix VBN cord-001249-awn9ayy6 321 8 with with IN cord-001249-awn9ayy6 321 9 3 3 CD cord-001249-awn9ayy6 321 10 % % NN cord-001249-awn9ayy6 322 1 PFA PFA NNP cord-001249-awn9ayy6 322 2 followed follow VBD cord-001249-awn9ayy6 322 3 by by IN cord-001249-awn9ayy6 322 4 ice ice NN cord-001249-awn9ayy6 322 5 cold cold JJ cord-001249-awn9ayy6 322 6 methanol methanol NN cord-001249-awn9ayy6 322 7 , , , cord-001249-awn9ayy6 322 8 and and CC cord-001249-awn9ayy6 322 9 incubated incubate VBN cord-001249-awn9ayy6 322 10 with with IN cord-001249-awn9ayy6 322 11 mouse mouse NN cord-001249-awn9ayy6 322 12 anti anti NNP cord-001249-awn9ayy6 322 13 - - NNP cord-001249-awn9ayy6 322 14 GM130 gm130 JJ cord-001249-awn9ayy6 322 15 ( ( -LRB- cord-001249-awn9ayy6 322 16 cisGolgi cisGolgi NNP cord-001249-awn9ayy6 322 17 ) ) -RRB- cord-001249-awn9ayy6 322 18 antibody antibody NN cord-001249-awn9ayy6 322 19 , , , cord-001249-awn9ayy6 322 20 followed follow VBN cord-001249-awn9ayy6 322 21 by by IN cord-001249-awn9ayy6 322 22 Alexa Alexa NNP cord-001249-awn9ayy6 322 23 Fluor Fluor NNP cord-001249-awn9ayy6 322 24 568 568 CD cord-001249-awn9ayy6 322 25 goat goat NN cord-001249-awn9ayy6 322 26 anti anti JJ cord-001249-awn9ayy6 322 27 - - JJ cord-001249-awn9ayy6 322 28 mouse mouse JJ cord-001249-awn9ayy6 322 29 IgG IgG NNP cord-001249-awn9ayy6 322 30 ( ( -LRB- cord-001249-awn9ayy6 322 31 red red NNP cord-001249-awn9ayy6 322 32 ) ) -RRB- cord-001249-awn9ayy6 322 33 . . . cord-001249-awn9ayy6 323 1 Then then RB cord-001249-awn9ayy6 323 2 plasmid plasmid NN cord-001249-awn9ayy6 323 3 - - HYPH cord-001249-awn9ayy6 323 4 expressed express VBN cord-001249-awn9ayy6 323 5 proteins protein NNS cord-001249-awn9ayy6 323 6 were be VBD cord-001249-awn9ayy6 323 7 visualised visualise VBN cord-001249-awn9ayy6 323 8 with with IN cord-001249-awn9ayy6 323 9 anti anti JJ cord-001249-awn9ayy6 323 10 - - JJ cord-001249-awn9ayy6 323 11 V5 v5 JJ cord-001249-awn9ayy6 323 12 antibody antibody NN cord-001249-awn9ayy6 323 13 conjugated conjugate VBN cord-001249-awn9ayy6 323 14 to to IN cord-001249-awn9ayy6 323 15 Alexa Alexa NNP cord-001249-awn9ayy6 323 16 Fluor Fluor NNP cord-001249-awn9ayy6 323 17 488 488 CD cord-001249-awn9ayy6 323 18 ( ( -LRB- cord-001249-awn9ayy6 323 19 green green NNP cord-001249-awn9ayy6 323 20 ) ) -RRB- cord-001249-awn9ayy6 323 21 . . . cord-001249-awn9ayy6 324 1 Nuclei nucleus NNS cord-001249-awn9ayy6 324 2 were be VBD cord-001249-awn9ayy6 324 3 counterstained counterstaine VBN cord-001249-awn9ayy6 324 4 using use VBG cord-001249-awn9ayy6 324 5 DAPI DAPI NNP cord-001249-awn9ayy6 324 6 ( ( -LRB- cord-001249-awn9ayy6 324 7 blue blue JJ cord-001249-awn9ayy6 324 8 ) ) -RRB- cord-001249-awn9ayy6 324 9 . . . cord-001249-awn9ayy6 325 1 Bars bar NNS cord-001249-awn9ayy6 325 2 correspond correspond VBP cord-001249-awn9ayy6 325 3 to to IN cord-001249-awn9ayy6 325 4 20 20 CD cord-001249-awn9ayy6 325 5 mm mm NNS cord-001249-awn9ayy6 325 6 . . . cord-001249-awn9ayy6 326 1 ( ( -LRB- cord-001249-awn9ayy6 326 2 TIF tif NN cord-001249-awn9ayy6 326 3 ) ) -RRB- cord-001249-awn9ayy6 326 4 Figure figure NN cord-001249-awn9ayy6 326 5 S6 s6 NN cord-001249-awn9ayy6 327 1 The the DT cord-001249-awn9ayy6 327 2 effect effect NN cord-001249-awn9ayy6 327 3 of of IN cord-001249-awn9ayy6 327 4 NSDV NSDV NNP cord-001249-awn9ayy6 327 5 protein protein NN cord-001249-awn9ayy6 327 6 expression expression NN cord-001249-awn9ayy6 327 7 on on IN cord-001249-awn9ayy6 327 8 PDI PDI NNP cord-001249-awn9ayy6 327 9 . . . cord-001249-awn9ayy6 328 1 Vero Vero NNP cord-001249-awn9ayy6 328 2 cells cell NNS cord-001249-awn9ayy6 328 3 were be VBD cord-001249-awn9ayy6 328 4 transfected transfecte VBN cord-001249-awn9ayy6 328 5 with with IN cord-001249-awn9ayy6 328 6 1 1 CD cord-001249-awn9ayy6 328 7 mg mg NN cord-001249-awn9ayy6 328 8 of of IN cord-001249-awn9ayy6 328 9 pcDNA6-GV pcDNA6-GV NNP cord-001249-awn9ayy6 328 10 - - HYPH cord-001249-awn9ayy6 328 11 N N NNP cord-001249-awn9ayy6 328 12 ( ( -LRB- cord-001249-awn9ayy6 328 13 ac ac NN cord-001249-awn9ayy6 328 14 ) ) -RRB- cord-001249-awn9ayy6 328 15 or or CC cord-001249-awn9ayy6 329 1 pcDNA6-GV pcDNA6-GV NNP cord-001249-awn9ayy6 329 2 - - HYPH cord-001249-awn9ayy6 329 3 La La NNP cord-001249-awn9ayy6 329 4 ( ( -LRB- cord-001249-awn9ayy6 329 5 d d NNP cord-001249-awn9ayy6 329 6 - - HYPH cord-001249-awn9ayy6 329 7 f f NNP cord-001249-awn9ayy6 329 8 ) ) -RRB- cord-001249-awn9ayy6 329 9 . . . cord-001249-awn9ayy6 330 1 After after IN cord-001249-awn9ayy6 330 2 24 24 CD cord-001249-awn9ayy6 330 3 h h NN cord-001249-awn9ayy6 330 4 , , , cord-001249-awn9ayy6 330 5 cells cell NNS cord-001249-awn9ayy6 330 6 were be VBD cord-001249-awn9ayy6 330 7 fixed fix VBN cord-001249-awn9ayy6 330 8 with with IN cord-001249-awn9ayy6 330 9 3 3 CD cord-001249-awn9ayy6 330 10 % % NN cord-001249-awn9ayy6 331 1 PFA PFA NNP cord-001249-awn9ayy6 331 2 followed follow VBD cord-001249-awn9ayy6 331 3 by by IN cord-001249-awn9ayy6 331 4 ice ice NN cord-001249-awn9ayy6 331 5 - - HYPH cord-001249-awn9ayy6 331 6 cold cold JJ cord-001249-awn9ayy6 331 7 methanol methanol NN cord-001249-awn9ayy6 331 8 , , , cord-001249-awn9ayy6 331 9 and and CC cord-001249-awn9ayy6 331 10 were be VBD cord-001249-awn9ayy6 331 11 stained stain VBN cord-001249-awn9ayy6 331 12 using use VBG cord-001249-awn9ayy6 331 13 mouse mouse NN cord-001249-awn9ayy6 331 14 anti anti JJ cord-001249-awn9ayy6 331 15 - - NNP cord-001249-awn9ayy6 331 16 PDI pdi JJ cord-001249-awn9ayy6 331 17 ( ( -LRB- cord-001249-awn9ayy6 331 18 clone clone NN cord-001249-awn9ayy6 331 19 1D3 1d3 CD cord-001249-awn9ayy6 331 20 ) ) -RRB- cord-001249-awn9ayy6 331 21 and and CC cord-001249-awn9ayy6 331 22 rabbit rabbit NN cord-001249-awn9ayy6 331 23 anti anti NNP cord-001249-awn9ayy6 331 24 - - NNP cord-001249-awn9ayy6 331 25 N N NNP cord-001249-awn9ayy6 331 26 ( ( -LRB- cord-001249-awn9ayy6 331 27 a a NNP cord-001249-awn9ayy6 331 28 - - HYPH cord-001249-awn9ayy6 331 29 c c NN cord-001249-awn9ayy6 331 30 ) ) -RRB- cord-001249-awn9ayy6 331 31 or or CC cord-001249-awn9ayy6 331 32 rabbit rabbit NN cord-001249-awn9ayy6 331 33 anti anti NNP cord-001249-awn9ayy6 331 34 - - NNP cord-001249-awn9ayy6 331 35 L l JJ cord-001249-awn9ayy6 331 36 ( ( -LRB- cord-001249-awn9ayy6 331 37 d d NN cord-001249-awn9ayy6 331 38 - - HYPH cord-001249-awn9ayy6 331 39 f f NNP cord-001249-awn9ayy6 331 40 ) ) -RRB- cord-001249-awn9ayy6 331 41 . . . cord-001249-awn9ayy6 332 1 Proteins protein NNS cord-001249-awn9ayy6 332 2 were be VBD cord-001249-awn9ayy6 332 3 visualised visualise VBN cord-001249-awn9ayy6 332 4 with with IN cord-001249-awn9ayy6 332 5 Alexa Alexa NNP cord-001249-awn9ayy6 332 6 Fluor Fluor NNP cord-001249-awn9ayy6 332 7 488 488 CD cord-001249-awn9ayy6 332 8 goat goat NN cord-001249-awn9ayy6 332 9 anti anti JJ cord-001249-awn9ayy6 332 10 - - JJ cord-001249-awn9ayy6 332 11 mouse mouse JJ cord-001249-awn9ayy6 332 12 IgG IgG NNP cord-001249-awn9ayy6 332 13 ( ( -LRB- cord-001249-awn9ayy6 332 14 green green NNP cord-001249-awn9ayy6 332 15 ) ) -RRB- cord-001249-awn9ayy6 332 16 and and CC cord-001249-awn9ayy6 332 17 Alexa Alexa NNP cord-001249-awn9ayy6 332 18 Fluor Fluor NNP cord-001249-awn9ayy6 332 19 568 568 CD cord-001249-awn9ayy6 332 20 goat goat NN cord-001249-awn9ayy6 332 21 anti anti JJ cord-001249-awn9ayy6 332 22 - - JJ cord-001249-awn9ayy6 332 23 rabbit rabbit JJ cord-001249-awn9ayy6 332 24 IgG igg NN cord-001249-awn9ayy6 332 25 ( ( -LRB- cord-001249-awn9ayy6 332 26 red red NNP cord-001249-awn9ayy6 332 27 ) ) -RRB- cord-001249-awn9ayy6 332 28 . . . cord-001249-awn9ayy6 333 1 Nuclei nucleus NNS cord-001249-awn9ayy6 333 2 were be VBD cord-001249-awn9ayy6 333 3 counterstained counterstaine VBN cord-001249-awn9ayy6 333 4 using use VBG cord-001249-awn9ayy6 333 5 DAPI DAPI NNP cord-001249-awn9ayy6 333 6 ( ( -LRB- cord-001249-awn9ayy6 333 7 blue blue JJ cord-001249-awn9ayy6 333 8 ) ) -RRB- cord-001249-awn9ayy6 333 9 . . . cord-001249-awn9ayy6 334 1 Bars bar NNS cord-001249-awn9ayy6 334 2 correspond correspond VBP cord-001249-awn9ayy6 334 3 to to IN cord-001249-awn9ayy6 334 4 40 40 CD cord-001249-awn9ayy6 334 5 mm mm NNS cord-001249-awn9ayy6 334 6 . . . cord-001249-awn9ayy6 335 1 ( ( -LRB- cord-001249-awn9ayy6 335 2 TIF TIF NNP cord-001249-awn9ayy6 335 3 ) ) -RRB- cord-001249-awn9ayy6 335 4 Nairobi Nairobi NNP cord-001249-awn9ayy6 335 5 Sheep Sheep NNP cord-001249-awn9ayy6 335 6 Disease Disease NNP cord-001249-awn9ayy6 336 1 On on IN cord-001249-awn9ayy6 336 2 a a DT cord-001249-awn9ayy6 336 3 tick tick NN cord-001249-awn9ayy6 336 4 - - HYPH cord-001249-awn9ayy6 336 5 borne bear VBN cord-001249-awn9ayy6 336 6 gastroenteritis gastroenteritis NN cord-001249-awn9ayy6 336 7 of of IN cord-001249-awn9ayy6 336 8 sheep sheep NNS cord-001249-awn9ayy6 336 9 and and CC cord-001249-awn9ayy6 336 10 goats goat NNS cord-001249-awn9ayy6 336 11 occurring occur VBG cord-001249-awn9ayy6 336 12 in in IN cord-001249-awn9ayy6 336 13 British British NNP cord-001249-awn9ayy6 336 14 East East NNP cord-001249-awn9ayy6 336 15 Africa Africa NNP cord-001249-awn9ayy6 337 1 A a DT cord-001249-awn9ayy6 337 2 survey survey NN cord-001249-awn9ayy6 337 3 of of IN cord-001249-awn9ayy6 337 4 Nairobi Nairobi NNP cord-001249-awn9ayy6 337 5 sheep sheep NN cord-001249-awn9ayy6 337 6 disease disease NN cord-001249-awn9ayy6 337 7 antibody antibody NN cord-001249-awn9ayy6 337 8 in in IN cord-001249-awn9ayy6 337 9 sheep sheep NNS cord-001249-awn9ayy6 337 10 and and CC cord-001249-awn9ayy6 337 11 goats goat NNS cord-001249-awn9ayy6 337 12 , , , cord-001249-awn9ayy6 337 13 wild wild JJ cord-001249-awn9ayy6 337 14 ruminants ruminant NNS cord-001249-awn9ayy6 337 15 and and CC cord-001249-awn9ayy6 337 16 rodents rodent NNS cord-001249-awn9ayy6 337 17 within within IN cord-001249-awn9ayy6 337 18 Kenya Kenya NNP cord-001249-awn9ayy6 337 19 Nairobi Nairobi NNP cord-001249-awn9ayy6 337 20 sheep sheep NN cord-001249-awn9ayy6 337 21 disease disease NN cord-001249-awn9ayy6 337 22 in in IN cord-001249-awn9ayy6 337 23 Kenya Kenya NNP cord-001249-awn9ayy6 337 24 . . . cord-001249-awn9ayy6 338 1 The the DT cord-001249-awn9ayy6 338 2 isolation isolation NN cord-001249-awn9ayy6 338 3 of of IN cord-001249-awn9ayy6 338 4 virus virus NN cord-001249-awn9ayy6 338 5 from from IN cord-001249-awn9ayy6 338 6 sheep sheep NNS cord-001249-awn9ayy6 338 7 and and CC cord-001249-awn9ayy6 338 8 goats goat NNS cord-001249-awn9ayy6 338 9 , , , cord-001249-awn9ayy6 338 10 ticks tick NNS cord-001249-awn9ayy6 338 11 and and CC cord-001249-awn9ayy6 338 12 possible possible JJ cord-001249-awn9ayy6 338 13 maintenance maintenance NN cord-001249-awn9ayy6 338 14 hosts host NNS cord-001249-awn9ayy6 339 1 An an DT cord-001249-awn9ayy6 339 2 epizootic epizootic NN cord-001249-awn9ayy6 339 3 of of IN cord-001249-awn9ayy6 339 4 Nairobi Nairobi NNP cord-001249-awn9ayy6 339 5 sheep sheep NN cord-001249-awn9ayy6 339 6 disease disease NN cord-001249-awn9ayy6 339 7 in in IN cord-001249-awn9ayy6 339 8 Uganda Uganda NNP cord-001249-awn9ayy6 339 9 Investigation Investigation NNP cord-001249-awn9ayy6 339 10 of of IN cord-001249-awn9ayy6 339 11 tick tick NN cord-001249-awn9ayy6 339 12 - - HYPH cord-001249-awn9ayy6 339 13 borne bear VBN cord-001249-awn9ayy6 339 14 viruses virus NNS cord-001249-awn9ayy6 339 15 as as IN cord-001249-awn9ayy6 339 16 pathogens pathogen NNS cord-001249-awn9ayy6 339 17 of of IN cord-001249-awn9ayy6 339 18 humans human NNS cord-001249-awn9ayy6 339 19 in in IN cord-001249-awn9ayy6 339 20 South South NNP cord-001249-awn9ayy6 339 21 Africa Africa NNP cord-001249-awn9ayy6 339 22 and and CC cord-001249-awn9ayy6 339 23 evidence evidence NN cord-001249-awn9ayy6 339 24 of of IN cord-001249-awn9ayy6 339 25 Dugbe Dugbe NNP cord-001249-awn9ayy6 339 26 virus virus NN cord-001249-awn9ayy6 339 27 infection infection NN cord-001249-awn9ayy6 339 28 in in IN cord-001249-awn9ayy6 339 29 a a DT cord-001249-awn9ayy6 339 30 patient patient NN cord-001249-awn9ayy6 339 31 with with IN cord-001249-awn9ayy6 339 32 prolonged prolonged JJ cord-001249-awn9ayy6 339 33 thrombocytopenia thrombocytopenia NN cord-001249-awn9ayy6 339 34 Nairobi Nairobi NNP cord-001249-awn9ayy6 339 35 sheep sheep NN cord-001249-awn9ayy6 339 36 disease disease NN cord-001249-awn9ayy6 339 37 virus virus NN cord-001249-awn9ayy6 339 38 Ganjam Ganjam NNP cord-001249-awn9ayy6 339 39 virus virus NN cord-001249-awn9ayy6 340 1 The the DT cord-001249-awn9ayy6 340 2 serological serological JJ cord-001249-awn9ayy6 340 3 relationships relationship NNS cord-001249-awn9ayy6 340 4 of of IN cord-001249-awn9ayy6 340 5 Nairobi Nairobi NNP cord-001249-awn9ayy6 340 6 sheep sheep NN cord-001249-awn9ayy6 340 7 disease disease NN cord-001249-awn9ayy6 340 8 virus virus NN cord-001249-awn9ayy6 340 9 Nairobi Nairobi NNP cord-001249-awn9ayy6 340 10 sheep sheep NN cord-001249-awn9ayy6 340 11 disease disease NN cord-001249-awn9ayy6 340 12 virus virus NN cord-001249-awn9ayy6 340 13 , , , cord-001249-awn9ayy6 340 14 an an DT cord-001249-awn9ayy6 340 15 important important JJ cord-001249-awn9ayy6 340 16 tickborne tickborne JJ cord-001249-awn9ayy6 340 17 pathogen pathogen NN cord-001249-awn9ayy6 340 18 of of IN cord-001249-awn9ayy6 340 19 sheep sheep NNS cord-001249-awn9ayy6 340 20 and and CC cord-001249-awn9ayy6 340 21 goats goat NNS cord-001249-awn9ayy6 340 22 in in IN cord-001249-awn9ayy6 340 23 Africa Africa NNP cord-001249-awn9ayy6 340 24 , , , cord-001249-awn9ayy6 340 25 is be VBZ cord-001249-awn9ayy6 340 26 also also RB cord-001249-awn9ayy6 340 27 present present JJ cord-001249-awn9ayy6 340 28 in in IN cord-001249-awn9ayy6 340 29 Asia Asia NNP cord-001249-awn9ayy6 340 30 Isolation Isolation NNP cord-001249-awn9ayy6 340 31 of of IN cord-001249-awn9ayy6 340 32 Ganjam Ganjam NNP cord-001249-awn9ayy6 340 33 virus virus NN cord-001249-awn9ayy6 340 34 from from IN cord-001249-awn9ayy6 340 35 ticks tick NNS cord-001249-awn9ayy6 341 1 Haemaphysalis Haemaphysalis NNP cord-001249-awn9ayy6 341 2 intermedia intermedia NN cord-001249-awn9ayy6 341 3 Ganjam Ganjam NNP cord-001249-awn9ayy6 341 4 virus virus NN cord-001249-awn9ayy6 341 5 : : : cord-001249-awn9ayy6 341 6 a a DT cord-001249-awn9ayy6 341 7 new new JJ cord-001249-awn9ayy6 341 8 arbovirus arbovirus NN cord-001249-awn9ayy6 341 9 isolated isolate VBN cord-001249-awn9ayy6 341 10 from from IN cord-001249-awn9ayy6 341 11 ticks tick NNS cord-001249-awn9ayy6 342 1 Haemaphysalis Haemaphysalis NNP cord-001249-awn9ayy6 342 2 intermedia intermedia NN cord-001249-awn9ayy6 342 3 Warburton Warburton NNP cord-001249-awn9ayy6 342 4 and and CC cord-001249-awn9ayy6 342 5 Nuttall Nuttall NNP cord-001249-awn9ayy6 342 6 , , , cord-001249-awn9ayy6 342 7 1909 1909 CD cord-001249-awn9ayy6 342 8 in in IN cord-001249-awn9ayy6 342 9 Orissa Orissa NNP cord-001249-awn9ayy6 342 10 Isolation Isolation NNP cord-001249-awn9ayy6 342 11 of of IN cord-001249-awn9ayy6 342 12 Ganjam Ganjam NNP cord-001249-awn9ayy6 342 13 virus virus NN cord-001249-awn9ayy6 342 14 from from IN cord-001249-awn9ayy6 342 15 ticks tick NNS cord-001249-awn9ayy6 342 16 collected collect VBN cord-001249-awn9ayy6 342 17 off off RP cord-001249-awn9ayy6 342 18 domestic domestic JJ cord-001249-awn9ayy6 342 19 animals animal NNS cord-001249-awn9ayy6 342 20 around around IN cord-001249-awn9ayy6 342 21 Pune Pune NNP cord-001249-awn9ayy6 342 22 Nairobi Nairobi NNP cord-001249-awn9ayy6 342 23 Sheep Sheep NNP cord-001249-awn9ayy6 342 24 Disease Disease NNP cord-001249-awn9ayy6 342 25 Qualitative Qualitative NNP cord-001249-awn9ayy6 342 26 studies study NNS cord-001249-awn9ayy6 342 27 of of IN cord-001249-awn9ayy6 342 28 the the DT cord-001249-awn9ayy6 342 29 transmission transmission NN cord-001249-awn9ayy6 342 30 of of IN cord-001249-awn9ayy6 342 31 Nairobi Nairobi NNP cord-001249-awn9ayy6 342 32 sheep sheep NN cord-001249-awn9ayy6 342 33 disease disease NN cord-001249-awn9ayy6 342 34 virus virus NN cord-001249-awn9ayy6 342 35 by by IN cord-001249-awn9ayy6 342 36 Rhipicephalus Rhipicephalus NNP cord-001249-awn9ayy6 342 37 appendiculatus appendiculatus NN cord-001249-awn9ayy6 342 38 ( ( -LRB- cord-001249-awn9ayy6 342 39 Ixodoidea Ixodoidea NNP cord-001249-awn9ayy6 342 40 , , , cord-001249-awn9ayy6 343 1 ixodidae ixodidae NNP cord-001249-awn9ayy6 343 2 ) ) -RRB- cord-001249-awn9ayy6 344 1 The the DT cord-001249-awn9ayy6 344 2 antibody antibody NN cord-001249-awn9ayy6 344 3 response response NN cord-001249-awn9ayy6 344 4 of of IN cord-001249-awn9ayy6 344 5 sheep sheep NN cord-001249-awn9ayy6 344 6 following follow VBG cord-001249-awn9ayy6 344 7 infection infection NN cord-001249-awn9ayy6 344 8 with with IN cord-001249-awn9ayy6 344 9 Nairobi Nairobi NNP cord-001249-awn9ayy6 344 10 sheep sheep NN cord-001249-awn9ayy6 344 11 disease disease NN cord-001249-awn9ayy6 344 12 virus virus NN cord-001249-awn9ayy6 345 1 The the DT cord-001249-awn9ayy6 345 2 propagation propagation NN cord-001249-awn9ayy6 345 3 of of IN cord-001249-awn9ayy6 345 4 Nairobi Nairobi NNP cord-001249-awn9ayy6 345 5 sheep sheep NN cord-001249-awn9ayy6 345 6 diseases disease NNS cord-001249-awn9ayy6 345 7 virus virus NN cord-001249-awn9ayy6 345 8 in in IN cord-001249-awn9ayy6 345 9 tissue tissue NN cord-001249-awn9ayy6 345 10 culture culture NN cord-001249-awn9ayy6 345 11 Crimean Crimean NNP cord-001249-awn9ayy6 345 12 - - HYPH cord-001249-awn9ayy6 345 13 Congo Congo NNP cord-001249-awn9ayy6 345 14 hemorrhagic hemorrhagic JJ cord-001249-awn9ayy6 345 15 fever fever NN cord-001249-awn9ayy6 345 16 Crimean Crimean NNP cord-001249-awn9ayy6 345 17 - - HYPH cord-001249-awn9ayy6 345 18 Congo Congo NNP cord-001249-awn9ayy6 345 19 haemorrhagic haemorrhagic JJ cord-001249-awn9ayy6 345 20 fever fever NN cord-001249-awn9ayy6 346 1 First first JJ cord-001249-awn9ayy6 346 2 documentation documentation NN cord-001249-awn9ayy6 346 3 of of IN cord-001249-awn9ayy6 346 4 human human JJ cord-001249-awn9ayy6 346 5 Crimean Crimean NNP cord-001249-awn9ayy6 346 6 - - HYPH cord-001249-awn9ayy6 346 7 Congo Congo NNP cord-001249-awn9ayy6 346 8 hemorrhagic hemorrhagic JJ cord-001249-awn9ayy6 346 9 fever fever NN cord-001249-awn9ayy6 346 10 Crimean Crimean NNP cord-001249-awn9ayy6 346 11 - - HYPH cord-001249-awn9ayy6 346 12 Congo Congo NNP cord-001249-awn9ayy6 346 13 hemorrhagic hemorrhagic JJ cord-001249-awn9ayy6 346 14 fever fever NN cord-001249-awn9ayy6 346 15 in in IN cord-001249-awn9ayy6 346 16 Kosovo Kosovo NNP cord-001249-awn9ayy6 346 17 Crimean Crimean NNP cord-001249-awn9ayy6 346 18 - - HYPH cord-001249-awn9ayy6 346 19 Congo Congo NNP cord-001249-awn9ayy6 346 20 haemorrhagic haemorrhagic JJ cord-001249-awn9ayy6 346 21 fever fever NN cord-001249-awn9ayy6 346 22 virus virus NN cord-001249-awn9ayy6 346 23 infection infection NN cord-001249-awn9ayy6 346 24 in in IN cord-001249-awn9ayy6 346 25 the the DT cord-001249-awn9ayy6 346 26 western western JJ cord-001249-awn9ayy6 346 27 province province NN cord-001249-awn9ayy6 346 28 of of IN cord-001249-awn9ayy6 346 29 Saudi Saudi NNP cord-001249-awn9ayy6 346 30 Arabia Arabia NNP cord-001249-awn9ayy6 346 31 Nosocomial Nosocomial NNP cord-001249-awn9ayy6 346 32 outbreak outbreak NN cord-001249-awn9ayy6 346 33 of of IN cord-001249-awn9ayy6 346 34 viral viral JJ cord-001249-awn9ayy6 346 35 hemorrhagic hemorrhagic JJ cord-001249-awn9ayy6 346 36 fever fever NN cord-001249-awn9ayy6 346 37 caused cause VBN cord-001249-awn9ayy6 346 38 by by IN cord-001249-awn9ayy6 346 39 Crimean crimean JJ cord-001249-awn9ayy6 346 40 Hemorrhagic Hemorrhagic NNP cord-001249-awn9ayy6 346 41 fever fever NN cord-001249-awn9ayy6 346 42 - - : cord-001249-awn9ayy6 346 43 Congo Congo NNP cord-001249-awn9ayy6 346 44 virus virus NN cord-001249-awn9ayy6 346 45 in in IN cord-001249-awn9ayy6 346 46 Pakistan Pakistan NNP cord-001249-awn9ayy6 346 47 Crimean Crimean NNP cord-001249-awn9ayy6 346 48 - - HYPH cord-001249-awn9ayy6 346 49 Congo Congo NNP cord-001249-awn9ayy6 346 50 hemorrhagic hemorrhagic JJ cord-001249-awn9ayy6 346 51 fever fever NN cord-001249-awn9ayy6 346 52 in in IN cord-001249-awn9ayy6 346 53 Bulgaria Bulgaria NNP cord-001249-awn9ayy6 346 54 Emergence Emergence NNP cord-001249-awn9ayy6 346 55 of of IN cord-001249-awn9ayy6 346 56 Crimean Crimean NNP cord-001249-awn9ayy6 346 57 - - HYPH cord-001249-awn9ayy6 346 58 Congo Congo NNP cord-001249-awn9ayy6 346 59 haemorrhagic haemorrhagic JJ cord-001249-awn9ayy6 346 60 fever fever NN cord-001249-awn9ayy6 346 61 in in IN cord-001249-awn9ayy6 346 62 Greece Greece NNP cord-001249-awn9ayy6 346 63 Human Human NNP cord-001249-awn9ayy6 346 64 Crimean Crimean NNP cord-001249-awn9ayy6 346 65 - - HYPH cord-001249-awn9ayy6 346 66 Congo Congo NNP cord-001249-awn9ayy6 346 67 hemorrhagic hemorrhagic JJ cord-001249-awn9ayy6 346 68 fever fever NN cord-001249-awn9ayy6 346 69 Crimean Crimean NNP cord-001249-awn9ayy6 346 70 - - HYPH cord-001249-awn9ayy6 346 71 congo congo NNP cord-001249-awn9ayy6 346 72 haemorrhagic haemorrhagic JJ cord-001249-awn9ayy6 346 73 fever fever NN cord-001249-awn9ayy6 346 74 : : : cord-001249-awn9ayy6 346 75 a a DT cord-001249-awn9ayy6 346 76 seroepidemiological seroepidemiological JJ cord-001249-awn9ayy6 346 77 and and CC cord-001249-awn9ayy6 346 78 tick tick VB cord-001249-awn9ayy6 346 79 survey survey NN cord-001249-awn9ayy6 346 80 in in IN cord-001249-awn9ayy6 346 81 the the DT cord-001249-awn9ayy6 346 82 Sultanate Sultanate NNP cord-001249-awn9ayy6 346 83 of of IN cord-001249-awn9ayy6 346 84 Oman Oman NNP cord-001249-awn9ayy6 346 85 Genetic genetic JJ cord-001249-awn9ayy6 346 86 characterization characterization NN cord-001249-awn9ayy6 346 87 of of IN cord-001249-awn9ayy6 346 88 the the DT cord-001249-awn9ayy6 346 89 M M NNP cord-001249-awn9ayy6 346 90 RNA RNA NNP cord-001249-awn9ayy6 346 91 segment segment NN cord-001249-awn9ayy6 346 92 of of IN cord-001249-awn9ayy6 346 93 Crimean crimean JJ cord-001249-awn9ayy6 346 94 Congo Congo NNP cord-001249-awn9ayy6 346 95 hemorrhagic hemorrhagic JJ cord-001249-awn9ayy6 346 96 fever fever NN cord-001249-awn9ayy6 346 97 virus virus NN cord-001249-awn9ayy6 346 98 strains strain NNS cord-001249-awn9ayy6 347 1 Ganjam ganjam JJ cord-001249-awn9ayy6 347 2 virus virus NN cord-001249-awn9ayy6 347 3 / / SYM cord-001249-awn9ayy6 347 4 Nairobi Nairobi NNP cord-001249-awn9ayy6 347 5 sheep sheep NN cord-001249-awn9ayy6 347 6 disease disease NN cord-001249-awn9ayy6 347 7 virus virus NN cord-001249-awn9ayy6 347 8 induces induce VBZ cord-001249-awn9ayy6 347 9 a a DT cord-001249-awn9ayy6 347 10 pro pro JJ cord-001249-awn9ayy6 347 11 - - JJ cord-001249-awn9ayy6 347 12 inflammatory inflammatory JJ cord-001249-awn9ayy6 347 13 response response NN cord-001249-awn9ayy6 347 14 in in IN cord-001249-awn9ayy6 347 15 infected infected JJ cord-001249-awn9ayy6 347 16 sheep sheep NN cord-001249-awn9ayy6 347 17 Immunohistochemical Immunohistochemical NNP cord-001249-awn9ayy6 347 18 and and CC cord-001249-awn9ayy6 347 19 in in IN cord-001249-awn9ayy6 347 20 situ situ JJ cord-001249-awn9ayy6 347 21 localization localization NN cord-001249-awn9ayy6 347 22 of of IN cord-001249-awn9ayy6 347 23 Crimean Crimean NNP cord-001249-awn9ayy6 347 24 - - HYPH cord-001249-awn9ayy6 347 25 Congo Congo NNP cord-001249-awn9ayy6 347 26 hemorrhagic hemorrhagic JJ cord-001249-awn9ayy6 347 27 fever fever NN cord-001249-awn9ayy6 347 28 ( ( -LRB- cord-001249-awn9ayy6 347 29 CCHF CCHF NNP cord-001249-awn9ayy6 347 30 ) ) -RRB- cord-001249-awn9ayy6 347 31 virus virus NN cord-001249-awn9ayy6 347 32 in in IN cord-001249-awn9ayy6 347 33 human human JJ cord-001249-awn9ayy6 347 34 tissues tissue NNS cord-001249-awn9ayy6 347 35 and and CC cord-001249-awn9ayy6 347 36 implications implication NNS cord-001249-awn9ayy6 347 37 for for IN cord-001249-awn9ayy6 347 38 CCHF CCHF NNP cord-001249-awn9ayy6 347 39 pathogenesis pathogenesis NN cord-001249-awn9ayy6 348 1 A a DT cord-001249-awn9ayy6 348 2 nosocomial nosocomial JJ cord-001249-awn9ayy6 348 3 outbreak outbreak NN cord-001249-awn9ayy6 348 4 of of IN cord-001249-awn9ayy6 348 5 Crimean Crimean NNP cord-001249-awn9ayy6 348 6 - - HYPH cord-001249-awn9ayy6 348 7 Congo Congo NNP cord-001249-awn9ayy6 348 8 haemorrhagic haemorrhagic JJ cord-001249-awn9ayy6 348 9 fever fever NN cord-001249-awn9ayy6 348 10 at at IN cord-001249-awn9ayy6 348 11 Tygerberg Tygerberg NNP cord-001249-awn9ayy6 348 12 Hospital Hospital NNP cord-001249-awn9ayy6 348 13 . . . cord-001249-awn9ayy6 349 1 Part part NN cord-001249-awn9ayy6 349 2 III III NNP cord-001249-awn9ayy6 349 3 . . . cord-001249-awn9ayy6 350 1 Clinical clinical JJ cord-001249-awn9ayy6 350 2 pathology pathology NN cord-001249-awn9ayy6 350 3 and and CC cord-001249-awn9ayy6 350 4 pathogenesis pathogenesis NN cord-001249-awn9ayy6 350 5 Evidence evidence NN cord-001249-awn9ayy6 350 6 of of IN cord-001249-awn9ayy6 350 7 vascular vascular JJ cord-001249-awn9ayy6 350 8 endothelial endothelial JJ cord-001249-awn9ayy6 350 9 damage damage NN cord-001249-awn9ayy6 350 10 in in IN cord-001249-awn9ayy6 350 11 Crimean Crimean NNP cord-001249-awn9ayy6 350 12 - - HYPH cord-001249-awn9ayy6 350 13 Congo Congo NNP cord-001249-awn9ayy6 350 14 hemorrhagic hemorrhagic JJ cord-001249-awn9ayy6 350 15 fever fever NN cord-001249-awn9ayy6 350 16 Evaluation evaluation NN cord-001249-awn9ayy6 350 17 of of IN cord-001249-awn9ayy6 350 18 serum serum NN cord-001249-awn9ayy6 350 19 levels level NNS cord-001249-awn9ayy6 350 20 of of IN cord-001249-awn9ayy6 350 21 interleukin interleukin NN cord-001249-awn9ayy6 350 22 ( ( -LRB- cord-001249-awn9ayy6 350 23 IL)-6 IL)-6 NNP cord-001249-awn9ayy6 350 24 , , , cord-001249-awn9ayy6 350 25 IL-10 IL-10 NNP cord-001249-awn9ayy6 350 26 , , , cord-001249-awn9ayy6 350 27 and and CC cord-001249-awn9ayy6 350 28 tumor tumor NN cord-001249-awn9ayy6 350 29 necrosis necrosis NN cord-001249-awn9ayy6 350 30 factor factor NN cord-001249-awn9ayy6 350 31 - - HYPH cord-001249-awn9ayy6 350 32 alpha alpha NN cord-001249-awn9ayy6 350 33 in in IN cord-001249-awn9ayy6 350 34 patients patient NNS cord-001249-awn9ayy6 350 35 with with IN cord-001249-awn9ayy6 350 36 Crimean Crimean NNP cord-001249-awn9ayy6 350 37 - - HYPH cord-001249-awn9ayy6 350 38 Congo Congo NNP cord-001249-awn9ayy6 350 39 hemorrhagic hemorrhagic JJ cord-001249-awn9ayy6 350 40 fever fever NN cord-001249-awn9ayy6 350 41 Dugbe Dugbe NNP cord-001249-awn9ayy6 350 42 virus virus NN cord-001249-awn9ayy6 350 43 : : : cord-001249-awn9ayy6 350 44 a a DT cord-001249-awn9ayy6 350 45 tick tick VB cord-001249-awn9ayy6 350 46 - - HYPH cord-001249-awn9ayy6 350 47 borne bear VBN cord-001249-awn9ayy6 350 48 arbovirus arbovirus NN cord-001249-awn9ayy6 350 49 from from IN cord-001249-awn9ayy6 350 50 Nigeria Nigeria NNP cord-001249-awn9ayy6 350 51 Ultrastructural Ultrastructural NNP cord-001249-awn9ayy6 350 52 studies study NNS cord-001249-awn9ayy6 350 53 on on IN cord-001249-awn9ayy6 350 54 the the DT cord-001249-awn9ayy6 350 55 replication replication NN cord-001249-awn9ayy6 350 56 and and CC cord-001249-awn9ayy6 350 57 morphogenesis morphogenesis NN cord-001249-awn9ayy6 350 58 of of IN cord-001249-awn9ayy6 350 59 Nairobi Nairobi NNP cord-001249-awn9ayy6 350 60 sheep sheep NN cord-001249-awn9ayy6 350 61 disease disease NN cord-001249-awn9ayy6 350 62 virus virus NN cord-001249-awn9ayy6 350 63 , , , cord-001249-awn9ayy6 350 64 a a DT cord-001249-awn9ayy6 350 65 Nairovirus Nairovirus NNP cord-001249-awn9ayy6 350 66 Structure Structure NNP cord-001249-awn9ayy6 350 67 and and CC cord-001249-awn9ayy6 350 68 morphogenesis morphogenesis NN cord-001249-awn9ayy6 350 69 of of IN cord-001249-awn9ayy6 350 70 Dugbe Dugbe NNP cord-001249-awn9ayy6 350 71 virus virus NN cord-001249-awn9ayy6 350 72 ( ( -LRB- cord-001249-awn9ayy6 350 73 Bunyaviridae Bunyaviridae NNP cord-001249-awn9ayy6 350 74 , , , cord-001249-awn9ayy6 350 75 Nairovirus Nairovirus NNP cord-001249-awn9ayy6 350 76 ) ) -RRB- cord-001249-awn9ayy6 350 77 studied study VBN cord-001249-awn9ayy6 350 78 by by IN cord-001249-awn9ayy6 350 79 immunogold immunogold NNP cord-001249-awn9ayy6 350 80 electron electron NN cord-001249-awn9ayy6 350 81 microscopy microscopy NN cord-001249-awn9ayy6 350 82 of of IN cord-001249-awn9ayy6 350 83 ultrathin ultrathin JJ cord-001249-awn9ayy6 350 84 cryosections cryosection NNS cord-001249-awn9ayy6 350 85 Structural structural JJ cord-001249-awn9ayy6 350 86 characteristics characteristic NNS cord-001249-awn9ayy6 350 87 of of IN cord-001249-awn9ayy6 350 88 nairoviruses nairoviruse NNS cord-001249-awn9ayy6 350 89 ( ( -LRB- cord-001249-awn9ayy6 350 90 genus genus NN cord-001249-awn9ayy6 350 91 Nairovirus Nairovirus NNP cord-001249-awn9ayy6 350 92 , , , cord-001249-awn9ayy6 350 93 Bunyaviridae Bunyaviridae NNP cord-001249-awn9ayy6 350 94 ) ) -RRB- cord-001249-awn9ayy6 350 95 Molecular Molecular NNP cord-001249-awn9ayy6 350 96 Biology biology NN cord-001249-awn9ayy6 350 97 of of IN cord-001249-awn9ayy6 350 98 Nairoviruses Nairoviruses NNP cord-001249-awn9ayy6 351 1 The the DT cord-001249-awn9ayy6 351 2 proteins protein NNS cord-001249-awn9ayy6 351 3 and and CC cord-001249-awn9ayy6 351 4 RNAs rna NNS cord-001249-awn9ayy6 351 5 specified specify VBN cord-001249-awn9ayy6 351 6 by by IN cord-001249-awn9ayy6 351 7 Clo Clo NNP cord-001249-awn9ayy6 351 8 Mor Mor NNP cord-001249-awn9ayy6 351 9 virus virus NN cord-001249-awn9ayy6 351 10 , , , cord-001249-awn9ayy6 351 11 a a DT cord-001249-awn9ayy6 351 12 Scottish Scottish NNP cord-001249-awn9ayy6 351 13 Nairovirus Nairovirus NNP cord-001249-awn9ayy6 351 14 Ribonucleoproteins Ribonucleoproteins NNP cord-001249-awn9ayy6 351 15 of of IN cord-001249-awn9ayy6 351 16 Uukuniemi Uukuniemi NNP cord-001249-awn9ayy6 351 17 virus virus NN cord-001249-awn9ayy6 351 18 are be VBP cord-001249-awn9ayy6 351 19 circular circular JJ cord-001249-awn9ayy6 351 20 Circular circular JJ cord-001249-awn9ayy6 351 21 forms form NNS cord-001249-awn9ayy6 351 22 of of IN cord-001249-awn9ayy6 351 23 Uukuniemi Uukuniemi NNP cord-001249-awn9ayy6 351 24 virion virion NN cord-001249-awn9ayy6 351 25 RNA RNA NNP cord-001249-awn9ayy6 351 26 : : : cord-001249-awn9ayy6 351 27 an an DT cord-001249-awn9ayy6 351 28 electron electron NN cord-001249-awn9ayy6 351 29 microscopic microscopic JJ cord-001249-awn9ayy6 351 30 study study NN cord-001249-awn9ayy6 351 31 Dugbe Dugbe NNP cord-001249-awn9ayy6 351 32 nairovirus nairovirus NNP cord-001249-awn9ayy6 351 33 S S NNP cord-001249-awn9ayy6 351 34 segment segment NN cord-001249-awn9ayy6 351 35 : : : cord-001249-awn9ayy6 351 36 correction correction NN cord-001249-awn9ayy6 351 37 of of IN cord-001249-awn9ayy6 351 38 published publish VBN cord-001249-awn9ayy6 351 39 sequence sequence NN cord-001249-awn9ayy6 351 40 and and CC cord-001249-awn9ayy6 351 41 comparison comparison NN cord-001249-awn9ayy6 351 42 of of IN cord-001249-awn9ayy6 351 43 five five CD cord-001249-awn9ayy6 351 44 isolates isolate NNS cord-001249-awn9ayy6 352 1 Crimean Crimean NNP cord-001249-awn9ayy6 352 2 - - HYPH cord-001249-awn9ayy6 352 3 Congo Congo NNP cord-001249-awn9ayy6 352 4 hemorrhagic hemorrhagic JJ cord-001249-awn9ayy6 352 5 fever fever NN cord-001249-awn9ayy6 352 6 virus virus NN cord-001249-awn9ayy6 352 7 nucleoprotein nucleoprotein NNP cord-001249-awn9ayy6 352 8 reveals reveal VBZ cord-001249-awn9ayy6 352 9 endonuclease endonuclease NN cord-001249-awn9ayy6 352 10 activity activity NN cord-001249-awn9ayy6 352 11 in in IN cord-001249-awn9ayy6 352 12 bunyaviruses bunyaviruses NNP cord-001249-awn9ayy6 352 13 Structure Structure NNP cord-001249-awn9ayy6 352 14 , , , cord-001249-awn9ayy6 352 15 function function NN cord-001249-awn9ayy6 352 16 , , , cord-001249-awn9ayy6 352 17 and and CC cord-001249-awn9ayy6 352 18 evolution evolution NN cord-001249-awn9ayy6 352 19 of of IN cord-001249-awn9ayy6 352 20 the the DT cord-001249-awn9ayy6 352 21 Crimean Crimean NNP cord-001249-awn9ayy6 352 22 - - HYPH cord-001249-awn9ayy6 352 23 Congo Congo NNP cord-001249-awn9ayy6 352 24 hemorrhagic hemorrhagic JJ cord-001249-awn9ayy6 352 25 fever fever NN cord-001249-awn9ayy6 352 26 virus virus NN cord-001249-awn9ayy6 352 27 nucleocapsid nucleocapsid NN cord-001249-awn9ayy6 352 28 protein protein NN cord-001249-awn9ayy6 352 29 Structure Structure NNP cord-001249-awn9ayy6 352 30 of of IN cord-001249-awn9ayy6 352 31 Crimean Crimean NNP cord-001249-awn9ayy6 352 32 - - HYPH cord-001249-awn9ayy6 352 33 Congo Congo NNP cord-001249-awn9ayy6 352 34 hemorrhagic hemorrhagic JJ cord-001249-awn9ayy6 352 35 fever fever NN cord-001249-awn9ayy6 352 36 virus virus NN cord-001249-awn9ayy6 352 37 nucleoprotein nucleoprotein NNP cord-001249-awn9ayy6 352 38 : : : cord-001249-awn9ayy6 352 39 superhelical superhelical NNP cord-001249-awn9ayy6 352 40 homooligomers homooligomer NNS cord-001249-awn9ayy6 352 41 and and CC cord-001249-awn9ayy6 352 42 the the DT cord-001249-awn9ayy6 352 43 role role NN cord-001249-awn9ayy6 352 44 of of IN cord-001249-awn9ayy6 352 45 caspase-3 caspase-3 NNP cord-001249-awn9ayy6 352 46 cleavage cleavage NN cord-001249-awn9ayy6 352 47 Polypeptide Polypeptide NNP cord-001249-awn9ayy6 352 48 synthesis synthesis NN cord-001249-awn9ayy6 352 49 of of IN cord-001249-awn9ayy6 352 50 Dugbe Dugbe NNP cord-001249-awn9ayy6 352 51 virus virus NN cord-001249-awn9ayy6 352 52 , , , cord-001249-awn9ayy6 352 53 a a DT cord-001249-awn9ayy6 352 54 member member NN cord-001249-awn9ayy6 352 55 of of IN cord-001249-awn9ayy6 352 56 the the DT cord-001249-awn9ayy6 352 57 Nairovirus Nairovirus NNP cord-001249-awn9ayy6 352 58 genus genus NN cord-001249-awn9ayy6 352 59 of of IN cord-001249-awn9ayy6 352 60 the the DT cord-001249-awn9ayy6 352 61 Bunyaviridae Bunyaviridae NNP cord-001249-awn9ayy6 352 62 Identification Identification NNP cord-001249-awn9ayy6 352 63 of of IN cord-001249-awn9ayy6 352 64 a a DT cord-001249-awn9ayy6 352 65 novel novel JJ cord-001249-awn9ayy6 352 66 C c NN cord-001249-awn9ayy6 352 67 - - HYPH cord-001249-awn9ayy6 352 68 terminal terminal NN cord-001249-awn9ayy6 352 69 cleavage cleavage NN cord-001249-awn9ayy6 352 70 of of IN cord-001249-awn9ayy6 352 71 Crimean Crimean NNP cord-001249-awn9ayy6 352 72 - - HYPH cord-001249-awn9ayy6 352 73 Congo Congo NNP cord-001249-awn9ayy6 352 74 hemorrhagic hemorrhagic JJ cord-001249-awn9ayy6 352 75 fever fever NN cord-001249-awn9ayy6 352 76 virus virus NN cord-001249-awn9ayy6 352 77 PreGN pregn NN cord-001249-awn9ayy6 352 78 that that WDT cord-001249-awn9ayy6 352 79 leads lead VBZ cord-001249-awn9ayy6 352 80 to to IN cord-001249-awn9ayy6 352 81 generation generation NN cord-001249-awn9ayy6 352 82 of of IN cord-001249-awn9ayy6 352 83 an an DT cord-001249-awn9ayy6 352 84 NSM NSM NNP cord-001249-awn9ayy6 352 85 protein protein NN cord-001249-awn9ayy6 352 86 Cellular cellular JJ cord-001249-awn9ayy6 352 87 localization localization NN cord-001249-awn9ayy6 352 88 and and CC cord-001249-awn9ayy6 352 89 antigenic antigenic JJ cord-001249-awn9ayy6 352 90 characterization characterization NN cord-001249-awn9ayy6 352 91 of of IN cord-001249-awn9ayy6 352 92 crimean crimean NNP cord-001249-awn9ayy6 352 93 - - HYPH cord-001249-awn9ayy6 352 94 congo congo NNP cord-001249-awn9ayy6 352 95 hemorrhagic hemorrhagic JJ cord-001249-awn9ayy6 352 96 fever fever NN cord-001249-awn9ayy6 352 97 virus virus NN cord-001249-awn9ayy6 352 98 glycoproteins glycoprotein VBZ cord-001249-awn9ayy6 352 99 Dugbe Dugbe NNP cord-001249-awn9ayy6 352 100 Nairovirus Nairovirus NNP cord-001249-awn9ayy6 352 101 M M NNP cord-001249-awn9ayy6 352 102 RNA RNA NNP cord-001249-awn9ayy6 352 103 : : : cord-001249-awn9ayy6 352 104 nucleotide nucleotide JJ cord-001249-awn9ayy6 352 105 sequence sequence NN cord-001249-awn9ayy6 352 106 and and CC cord-001249-awn9ayy6 352 107 coding coding NN cord-001249-awn9ayy6 352 108 strategy strategy NN cord-001249-awn9ayy6 353 1 Characterization characterization NN cord-001249-awn9ayy6 353 2 of of IN cord-001249-awn9ayy6 353 3 the the DT cord-001249-awn9ayy6 353 4 glycoproteins glycoprotein NNS cord-001249-awn9ayy6 353 5 of of IN cord-001249-awn9ayy6 353 6 Crimean Crimean NNP cord-001249-awn9ayy6 353 7 - - HYPH cord-001249-awn9ayy6 353 8 Congo Congo NNP cord-001249-awn9ayy6 353 9 hemorrhagic hemorrhagic JJ cord-001249-awn9ayy6 353 10 fever fever NN cord-001249-awn9ayy6 353 11 virus virus NN cord-001249-awn9ayy6 353 12 Large Large NNP cord-001249-awn9ayy6 353 13 RNA RNA NNP cord-001249-awn9ayy6 353 14 segment segment NN cord-001249-awn9ayy6 353 15 of of IN cord-001249-awn9ayy6 353 16 Dugbe Dugbe NNP cord-001249-awn9ayy6 353 17 nairovirus nairovirus NNP cord-001249-awn9ayy6 353 18 encodes encode VBZ cord-001249-awn9ayy6 353 19 the the DT cord-001249-awn9ayy6 353 20 putative putative JJ cord-001249-awn9ayy6 353 21 RNA RNA NNP cord-001249-awn9ayy6 353 22 polymerase polymerase NN cord-001249-awn9ayy6 353 23 Crimean Crimean NNP cord-001249-awn9ayy6 353 24 - - HYPH cord-001249-awn9ayy6 353 25 Congo Congo NNP cord-001249-awn9ayy6 353 26 hemorrhagic hemorrhagic JJ cord-001249-awn9ayy6 353 27 fever fever NN cord-001249-awn9ayy6 353 28 virus virus NN cord-001249-awn9ayy6 353 29 genome genome NN cord-001249-awn9ayy6 353 30 L L NNP cord-001249-awn9ayy6 353 31 RNA RNA NNP cord-001249-awn9ayy6 353 32 segment segment NN cord-001249-awn9ayy6 353 33 and and CC cord-001249-awn9ayy6 353 34 encoded encode VBD cord-001249-awn9ayy6 353 35 protein protein NN cord-001249-awn9ayy6 353 36 Mechanism Mechanism NNP cord-001249-awn9ayy6 353 37 of of IN cord-001249-awn9ayy6 353 38 tripartite tripartite NNP cord-001249-awn9ayy6 353 39 RNA RNA NNP cord-001249-awn9ayy6 353 40 genome genome NN cord-001249-awn9ayy6 353 41 packaging packaging NN cord-001249-awn9ayy6 353 42 in in IN cord-001249-awn9ayy6 353 43 Rift Rift NNP cord-001249-awn9ayy6 353 44 Valley Valley NNP cord-001249-awn9ayy6 353 45 fever fever NN cord-001249-awn9ayy6 353 46 virus virus NN cord-001249-awn9ayy6 353 47 Role role NN cord-001249-awn9ayy6 353 48 of of IN cord-001249-awn9ayy6 353 49 actin actin NN cord-001249-awn9ayy6 353 50 filaments filament NNS cord-001249-awn9ayy6 353 51 in in IN cord-001249-awn9ayy6 353 52 targeting target VBG cord-001249-awn9ayy6 353 53 of of IN cord-001249-awn9ayy6 353 54 Crimean crimean JJ cord-001249-awn9ayy6 353 55 Congo Congo NNP cord-001249-awn9ayy6 353 56 hemorrhagic hemorrhagic JJ cord-001249-awn9ayy6 353 57 fever fever NN cord-001249-awn9ayy6 353 58 virus virus NN cord-001249-awn9ayy6 353 59 nucleocapsid nucleocapsid NN cord-001249-awn9ayy6 353 60 protein protein NN cord-001249-awn9ayy6 353 61 to to IN cord-001249-awn9ayy6 353 62 perinuclear perinuclear NNP cord-001249-awn9ayy6 353 63 regions region NNS cord-001249-awn9ayy6 353 64 of of IN cord-001249-awn9ayy6 353 65 mammalian mammalian JJ cord-001249-awn9ayy6 353 66 cells cell NNS cord-001249-awn9ayy6 353 67 Crimean Crimean NNP cord-001249-awn9ayy6 353 68 - - HYPH cord-001249-awn9ayy6 353 69 Congo Congo NNP cord-001249-awn9ayy6 353 70 hemorrhagic hemorrhagic JJ cord-001249-awn9ayy6 353 71 fever fever NN cord-001249-awn9ayy6 353 72 virus virus NN cord-001249-awn9ayy6 353 73 - - HYPH cord-001249-awn9ayy6 353 74 encoded encode VBN cord-001249-awn9ayy6 353 75 ovarian ovarian JJ cord-001249-awn9ayy6 353 76 tumor tumor NN cord-001249-awn9ayy6 353 77 protease protease NN cord-001249-awn9ayy6 353 78 activity activity NN cord-001249-awn9ayy6 353 79 is be VBZ cord-001249-awn9ayy6 353 80 dispensable dispensable JJ cord-001249-awn9ayy6 353 81 for for IN cord-001249-awn9ayy6 353 82 virus virus NN cord-001249-awn9ayy6 353 83 RNA rna NN cord-001249-awn9ayy6 353 84 polymerase polymerase NN cord-001249-awn9ayy6 353 85 function function NN cord-001249-awn9ayy6 353 86 Qalyub Qalyub NNP cord-001249-awn9ayy6 353 87 virus virus NN cord-001249-awn9ayy6 353 88 , , , cord-001249-awn9ayy6 353 89 a a DT cord-001249-awn9ayy6 353 90 member member NN cord-001249-awn9ayy6 353 91 of of IN cord-001249-awn9ayy6 353 92 the the DT cord-001249-awn9ayy6 353 93 newly newly RB cord-001249-awn9ayy6 353 94 proposed propose VBN cord-001249-awn9ayy6 353 95 Nairovirus Nairovirus NNP cord-001249-awn9ayy6 353 96 genus genus NN cord-001249-awn9ayy6 353 97 ( ( -LRB- cord-001249-awn9ayy6 353 98 Bunyavividae Bunyavividae NNP cord-001249-awn9ayy6 353 99 ) ) -RRB- cord-001249-awn9ayy6 353 100 Crimean Crimean NNP cord-001249-awn9ayy6 353 101 - - HYPH cord-001249-awn9ayy6 353 102 Congo Congo NNP cord-001249-awn9ayy6 353 103 hemorrhagic hemorrhagic JJ cord-001249-awn9ayy6 353 104 fever fever NN cord-001249-awn9ayy6 353 105 virus virus NN cord-001249-awn9ayy6 353 106 glycoprotein glycoprotein NN cord-001249-awn9ayy6 353 107 proteolytic proteolytic JJ cord-001249-awn9ayy6 353 108 processing processing NN cord-001249-awn9ayy6 353 109 by by IN cord-001249-awn9ayy6 353 110 subtilase subtilase NNP cord-001249-awn9ayy6 353 111 SKI-1 SKI-1 NNP cord-001249-awn9ayy6 353 112 Crimean Crimean NNP cord-001249-awn9ayy6 353 113 - - HYPH cord-001249-awn9ayy6 353 114 Congo Congo NNP cord-001249-awn9ayy6 353 115 hemorrhagic hemorrhagic JJ cord-001249-awn9ayy6 353 116 fever fever NN cord-001249-awn9ayy6 353 117 virus virus NN cord-001249-awn9ayy6 353 118 glycoprotein glycoprotein NN cord-001249-awn9ayy6 353 119 processing process VBG cord-001249-awn9ayy6 353 120 by by IN cord-001249-awn9ayy6 353 121 the the DT cord-001249-awn9ayy6 353 122 endoprotease endoprotease NN cord-001249-awn9ayy6 354 1 SKI-1/ ski-1/ NN cord-001249-awn9ayy6 355 1 S1P S1P NNP cord-001249-awn9ayy6 355 2 is be VBZ cord-001249-awn9ayy6 355 3 critical critical JJ cord-001249-awn9ayy6 355 4 for for IN cord-001249-awn9ayy6 355 5 virus virus NN cord-001249-awn9ayy6 355 6 infectivity infectivity NN cord-001249-awn9ayy6 355 7 Crimean Crimean NNP cord-001249-awn9ayy6 355 8 - - HYPH cord-001249-awn9ayy6 355 9 congo congo NNP cord-001249-awn9ayy6 355 10 hemorrhagic hemorrhagic JJ cord-001249-awn9ayy6 355 11 fever fever NN cord-001249-awn9ayy6 355 12 virus virus NN cord-001249-awn9ayy6 355 13 glycoprotein glycoprotein NN cord-001249-awn9ayy6 355 14 precursor precursor NN cord-001249-awn9ayy6 355 15 is be VBZ cord-001249-awn9ayy6 355 16 cleaved cleave VBN cord-001249-awn9ayy6 355 17 by by IN cord-001249-awn9ayy6 355 18 Furin Furin NNP cord-001249-awn9ayy6 355 19 - - HYPH cord-001249-awn9ayy6 355 20 like like JJ cord-001249-awn9ayy6 355 21 and and CC cord-001249-awn9ayy6 355 22 SKI-1 SKI-1 NNP cord-001249-awn9ayy6 355 23 proteases protease NNS cord-001249-awn9ayy6 355 24 to to TO cord-001249-awn9ayy6 355 25 generate generate VB cord-001249-awn9ayy6 355 26 a a DT cord-001249-awn9ayy6 355 27 novel novel JJ cord-001249-awn9ayy6 355 28 38-kilodalton 38-kilodalton CD cord-001249-awn9ayy6 355 29 glycoprotein glycoprotein NN cord-001249-awn9ayy6 355 30 Congo Congo NNP cord-001249-awn9ayy6 355 31 / / SYM cord-001249-awn9ayy6 355 32 Crimean crimean JJ cord-001249-awn9ayy6 355 33 haemorrhagic haemorrhagic JJ cord-001249-awn9ayy6 355 34 fever fever NN cord-001249-awn9ayy6 355 35 virus virus NN cord-001249-awn9ayy6 355 36 from from IN cord-001249-awn9ayy6 355 37 Iraq Iraq NNP cord-001249-awn9ayy6 355 38 1979 1979 CD cord-001249-awn9ayy6 355 39 : : : cord-001249-awn9ayy6 356 1 I. I. NNP cord-001249-awn9ayy6 356 2 Morphology morphology NN cord-001249-awn9ayy6 356 3 in in IN cord-001249-awn9ayy6 356 4 BHK21 BHK21 NNP cord-001249-awn9ayy6 356 5 cells cell VBZ cord-001249-awn9ayy6 356 6 Council Council NNP cord-001249-awn9ayy6 356 7 Directive Directive NNP cord-001249-awn9ayy6 356 8 86/609 86/609 CD cord-001249-awn9ayy6 356 9 / / SYM cord-001249-awn9ayy6 356 10 EEC EEC NNP cord-001249-awn9ayy6 356 11 of of IN cord-001249-awn9ayy6 356 12 24 24 CD cord-001249-awn9ayy6 356 13 November November NNP cord-001249-awn9ayy6 356 14 1986 1986 CD cord-001249-awn9ayy6 356 15 on on IN cord-001249-awn9ayy6 356 16 the the DT cord-001249-awn9ayy6 356 17 approximation approximation NN cord-001249-awn9ayy6 356 18 of of IN cord-001249-awn9ayy6 356 19 laws law NNS cord-001249-awn9ayy6 356 20 , , , cord-001249-awn9ayy6 356 21 regulations regulation NNS cord-001249-awn9ayy6 356 22 and and CC cord-001249-awn9ayy6 356 23 administrative administrative JJ cord-001249-awn9ayy6 356 24 provisions provision NNS cord-001249-awn9ayy6 356 25 of of IN cord-001249-awn9ayy6 356 26 the the DT cord-001249-awn9ayy6 356 27 Member Member NNP cord-001249-awn9ayy6 356 28 States States NNP cord-001249-awn9ayy6 356 29 regarding regard VBG cord-001249-awn9ayy6 356 30 the the DT cord-001249-awn9ayy6 356 31 protection protection NN cord-001249-awn9ayy6 356 32 of of IN cord-001249-awn9ayy6 356 33 animals animal NNS cord-001249-awn9ayy6 356 34 used use VBN cord-001249-awn9ayy6 356 35 for for IN cord-001249-awn9ayy6 356 36 experimental experimental JJ cord-001249-awn9ayy6 356 37 and and CC cord-001249-awn9ayy6 356 38 other other JJ cord-001249-awn9ayy6 356 39 scientific scientific JJ cord-001249-awn9ayy6 356 40 purposes purpose NNS cord-001249-awn9ayy6 357 1 Inhibition inhibition NN cord-001249-awn9ayy6 357 2 of of IN cord-001249-awn9ayy6 357 3 interferon interferon NN cord-001249-awn9ayy6 357 4 induction induction NN cord-001249-awn9ayy6 357 5 and and CC cord-001249-awn9ayy6 357 6 action action NN cord-001249-awn9ayy6 357 7 by by IN cord-001249-awn9ayy6 357 8 the the DT cord-001249-awn9ayy6 357 9 nairovirus nairovirus JJ cord-001249-awn9ayy6 357 10 Nairobi Nairobi NNP cord-001249-awn9ayy6 357 11 sheep sheep NN cord-001249-awn9ayy6 357 12 disease disease NN cord-001249-awn9ayy6 357 13 virus virus NN cord-001249-awn9ayy6 357 14 / / SYM cord-001249-awn9ayy6 357 15 Ganjam Ganjam NNP cord-001249-awn9ayy6 357 16 virus virus NN cord-001249-awn9ayy6 357 17 A a DT cord-001249-awn9ayy6 357 18 102 102 CD cord-001249-awn9ayy6 357 19 kDa kDa NNP cord-001249-awn9ayy6 357 20 subunit subunit NN cord-001249-awn9ayy6 357 21 of of IN cord-001249-awn9ayy6 357 22 a a DT cord-001249-awn9ayy6 357 23 Golgi Golgi NNP cord-001249-awn9ayy6 357 24 - - HYPH cord-001249-awn9ayy6 357 25 associated associate VBN cord-001249-awn9ayy6 357 26 particle particle NN cord-001249-awn9ayy6 357 27 has have VBZ cord-001249-awn9ayy6 357 28 homology homology NN cord-001249-awn9ayy6 357 29 to to IN cord-001249-awn9ayy6 357 30 beta beta JJ cord-001249-awn9ayy6 357 31 subunits subunit NNS cord-001249-awn9ayy6 357 32 of of IN cord-001249-awn9ayy6 357 33 trimeric trimeric JJ cord-001249-awn9ayy6 357 34 G g NN cord-001249-awn9ayy6 357 35 proteins protein NNS cord-001249-awn9ayy6 358 1 African african JJ cord-001249-awn9ayy6 358 2 swine swine NN cord-001249-awn9ayy6 358 3 fever fever NN cord-001249-awn9ayy6 358 4 virus virus NN cord-001249-awn9ayy6 358 5 is be VBZ cord-001249-awn9ayy6 358 6 wrapped wrap VBN cord-001249-awn9ayy6 358 7 by by IN cord-001249-awn9ayy6 358 8 the the DT cord-001249-awn9ayy6 358 9 endoplasmic endoplasmic NN cord-001249-awn9ayy6 358 10 reticulum reticulum NN cord-001249-awn9ayy6 358 11 Rinderpest Rinderpest NNP cord-001249-awn9ayy6 358 12 virus virus NN cord-001249-awn9ayy6 358 13 blocks block VBZ cord-001249-awn9ayy6 358 14 type type NN cord-001249-awn9ayy6 358 15 I -PRON- PRP cord-001249-awn9ayy6 358 16 and and CC cord-001249-awn9ayy6 358 17 type type NN cord-001249-awn9ayy6 358 18 II ii CD cord-001249-awn9ayy6 359 1 interferon interferon NNP cord-001249-awn9ayy6 359 2 action action NN cord-001249-awn9ayy6 359 3 : : : cord-001249-awn9ayy6 359 4 role role NN cord-001249-awn9ayy6 359 5 of of IN cord-001249-awn9ayy6 359 6 structural structural JJ cord-001249-awn9ayy6 359 7 and and CC cord-001249-awn9ayy6 359 8 nonstructural nonstructural JJ cord-001249-awn9ayy6 359 9 proteins protein NNS cord-001249-awn9ayy6 359 10 Characterization characterization NN cord-001249-awn9ayy6 359 11 of of IN cord-001249-awn9ayy6 359 12 a a DT cord-001249-awn9ayy6 359 13 cis cis NNP cord-001249-awn9ayy6 359 14 - - HYPH cord-001249-awn9ayy6 359 15 Golgi Golgi NNP cord-001249-awn9ayy6 359 16 matrix matrix NN cord-001249-awn9ayy6 359 17 protein protein NN cord-001249-awn9ayy6 359 18 , , , cord-001249-awn9ayy6 359 19 GM130 GM130 NNP cord-001249-awn9ayy6 359 20 Identification Identification NNP cord-001249-awn9ayy6 359 21 by by IN cord-001249-awn9ayy6 359 22 anti anti JJ cord-001249-awn9ayy6 359 23 - - JJ cord-001249-awn9ayy6 359 24 idiotype idiotype JJ cord-001249-awn9ayy6 359 25 antibodies antibody NNS cord-001249-awn9ayy6 359 26 of of IN cord-001249-awn9ayy6 359 27 an an DT cord-001249-awn9ayy6 359 28 intracellular intracellular JJ cord-001249-awn9ayy6 359 29 membrane membrane NN cord-001249-awn9ayy6 359 30 protein protein NN cord-001249-awn9ayy6 359 31 that that WDT cord-001249-awn9ayy6 359 32 recognizes recognize VBZ cord-001249-awn9ayy6 359 33 a a DT cord-001249-awn9ayy6 359 34 mammalian mammalian JJ cord-001249-awn9ayy6 359 35 endoplasmic endoplasmic NN cord-001249-awn9ayy6 359 36 reticulum reticulum NN cord-001249-awn9ayy6 359 37 retention retention NN cord-001249-awn9ayy6 359 38 signal signal NN cord-001249-awn9ayy6 359 39 Inhibition inhibition NN cord-001249-awn9ayy6 359 40 of of IN cord-001249-awn9ayy6 359 41 a a DT cord-001249-awn9ayy6 359 42 reductive reductive JJ cord-001249-awn9ayy6 359 43 function function NN cord-001249-awn9ayy6 359 44 of of IN cord-001249-awn9ayy6 359 45 the the DT cord-001249-awn9ayy6 359 46 plasma plasma NN cord-001249-awn9ayy6 359 47 membrane membrane NN cord-001249-awn9ayy6 359 48 by by IN cord-001249-awn9ayy6 359 49 bacitracin bacitracin NN cord-001249-awn9ayy6 359 50 and and CC cord-001249-awn9ayy6 359 51 antibodies antibody NNS cord-001249-awn9ayy6 359 52 against against IN cord-001249-awn9ayy6 359 53 protein protein NN cord-001249-awn9ayy6 359 54 disulfide disulfide NN cord-001249-awn9ayy6 359 55 - - HYPH cord-001249-awn9ayy6 359 56 isomerase isomerase NN cord-001249-awn9ayy6 359 57 Cell cell NN cord-001249-awn9ayy6 359 58 entry entry NN cord-001249-awn9ayy6 359 59 by by IN cord-001249-awn9ayy6 359 60 enveloped envelop VBN cord-001249-awn9ayy6 359 61 viruses virus NNS cord-001249-awn9ayy6 359 62 : : : cord-001249-awn9ayy6 359 63 redox redox JJ cord-001249-awn9ayy6 359 64 considerations consideration NNS cord-001249-awn9ayy6 359 65 for for IN cord-001249-awn9ayy6 360 1 HIV HIV NNP cord-001249-awn9ayy6 360 2 and and CC cord-001249-awn9ayy6 360 3 SARS SARS NNP cord-001249-awn9ayy6 360 4 - - HYPH cord-001249-awn9ayy6 360 5 coronavirus coronavirus NN cord-001249-awn9ayy6 360 6 Protein Protein NNP cord-001249-awn9ayy6 360 7 disulfide disulfide NN cord-001249-awn9ayy6 360 8 - - HYPH cord-001249-awn9ayy6 360 9 isomerase isomerase NN cord-001249-awn9ayy6 360 10 in in IN cord-001249-awn9ayy6 360 11 rat rat NN cord-001249-awn9ayy6 360 12 exocrine exocrine JJ cord-001249-awn9ayy6 360 13 pancreatic pancreatic NN cord-001249-awn9ayy6 360 14 cells cell NNS cord-001249-awn9ayy6 360 15 is be VBZ cord-001249-awn9ayy6 360 16 exported export VBN cord-001249-awn9ayy6 360 17 from from IN cord-001249-awn9ayy6 360 18 the the DT cord-001249-awn9ayy6 360 19 endoplasmic endoplasmic NN cord-001249-awn9ayy6 360 20 reticulum reticulum NN cord-001249-awn9ayy6 360 21 despite despite IN cord-001249-awn9ayy6 360 22 possessing possess VBG cord-001249-awn9ayy6 360 23 the the DT cord-001249-awn9ayy6 360 24 retention retention NN cord-001249-awn9ayy6 360 25 signal signal NN cord-001249-awn9ayy6 360 26 Rift Rift NNP cord-001249-awn9ayy6 360 27 Valley Valley NNP cord-001249-awn9ayy6 360 28 fever fever NN cord-001249-awn9ayy6 360 29 virus virus NN cord-001249-awn9ayy6 360 30 NSs NSs NNP cord-001249-awn9ayy6 360 31 mRNA mRNA NNP cord-001249-awn9ayy6 360 32 is be VBZ cord-001249-awn9ayy6 360 33 transcribed transcribe VBN cord-001249-awn9ayy6 360 34 from from IN cord-001249-awn9ayy6 360 35 an an DT cord-001249-awn9ayy6 360 36 incoming incoming JJ cord-001249-awn9ayy6 360 37 anti anti JJ cord-001249-awn9ayy6 360 38 - - JJ cord-001249-awn9ayy6 360 39 viral viral JJ cord-001249-awn9ayy6 360 40 - - HYPH cord-001249-awn9ayy6 360 41 sense sense NN cord-001249-awn9ayy6 360 42 S S NNP cord-001249-awn9ayy6 360 43 RNA RNA NNP cord-001249-awn9ayy6 360 44 segment segment NN cord-001249-awn9ayy6 361 1 The the DT cord-001249-awn9ayy6 361 2 ribonucleic ribonucleic JJ cord-001249-awn9ayy6 361 3 acids acid NNS cord-001249-awn9ayy6 361 4 of of IN cord-001249-awn9ayy6 361 5 Uukuniemi Uukuniemi NNP cord-001249-awn9ayy6 361 6 virus virus NN cord-001249-awn9ayy6 361 7 , , , cord-001249-awn9ayy6 361 8 a a DT cord-001249-awn9ayy6 361 9 noncubical noncubical JJ cord-001249-awn9ayy6 361 10 tick tick NN cord-001249-awn9ayy6 361 11 - - HYPH cord-001249-awn9ayy6 361 12 borne bear VBN cord-001249-awn9ayy6 361 13 arbovirus arbovirus NN cord-001249-awn9ayy6 361 14 The the DT cord-001249-awn9ayy6 361 15 virus virus NN cord-001249-awn9ayy6 361 16 particle particle NN cord-001249-awn9ayy6 361 17 nucleic nucleic VBZ cord-001249-awn9ayy6 361 18 acids acid NNS cord-001249-awn9ayy6 361 19 and and CC cord-001249-awn9ayy6 361 20 proteins protein NNS cord-001249-awn9ayy6 361 21 of of IN cord-001249-awn9ayy6 361 22 four four CD cord-001249-awn9ayy6 361 23 bunyaviruses bunyavirus NNS cord-001249-awn9ayy6 361 24 Secretion Secretion NNP cord-001249-awn9ayy6 361 25 , , , cord-001249-awn9ayy6 361 26 surface surface NN cord-001249-awn9ayy6 361 27 localization localization NN cord-001249-awn9ayy6 361 28 , , , cord-001249-awn9ayy6 361 29 turnover turnover NN cord-001249-awn9ayy6 361 30 , , , cord-001249-awn9ayy6 361 31 and and CC cord-001249-awn9ayy6 361 32 steady steady JJ cord-001249-awn9ayy6 361 33 state state NN cord-001249-awn9ayy6 361 34 expression expression NN cord-001249-awn9ayy6 361 35 of of IN cord-001249-awn9ayy6 361 36 protein protein NN cord-001249-awn9ayy6 361 37 disulfide disulfide NN cord-001249-awn9ayy6 361 38 isomerase isomerase NN cord-001249-awn9ayy6 361 39 in in IN cord-001249-awn9ayy6 361 40 rat rat NN cord-001249-awn9ayy6 361 41 hepatocytes hepatocyte NNS cord-001249-awn9ayy6 361 42 SignalP signalp VBP cord-001249-awn9ayy6 361 43 4.0 4.0 CD cord-001249-awn9ayy6 361 44 : : : cord-001249-awn9ayy6 361 45 discriminating discriminate VBG cord-001249-awn9ayy6 361 46 signal signal NN cord-001249-awn9ayy6 361 47 peptides peptide NNS cord-001249-awn9ayy6 361 48 from from IN cord-001249-awn9ayy6 361 49 transmembrane transmembrane NN cord-001249-awn9ayy6 361 50 regions region NNS cord-001249-awn9ayy6 361 51 Dynein Dynein NNP cord-001249-awn9ayy6 361 52 - - HYPH cord-001249-awn9ayy6 361 53 dependent dependent JJ cord-001249-awn9ayy6 361 54 transport transport NN cord-001249-awn9ayy6 361 55 of of IN cord-001249-awn9ayy6 361 56 the the DT cord-001249-awn9ayy6 361 57 hantaan hantaan NNP cord-001249-awn9ayy6 361 58 virus virus NN cord-001249-awn9ayy6 361 59 nucleocapsid nucleocapsid NN cord-001249-awn9ayy6 361 60 protein protein NN cord-001249-awn9ayy6 361 61 to to IN cord-001249-awn9ayy6 361 62 the the DT cord-001249-awn9ayy6 361 63 endoplasmic endoplasmic NN cord-001249-awn9ayy6 361 64 reticulum reticulum NN cord-001249-awn9ayy6 361 65 - - : cord-001249-awn9ayy6 361 66 Golgi Golgi NNP cord-001249-awn9ayy6 361 67 intermediate intermediate JJ cord-001249-awn9ayy6 361 68 compartment compartment NN cord-001249-awn9ayy6 361 69 Microtubuledependent Microtubuledependent NNP cord-001249-awn9ayy6 361 70 and and CC cord-001249-awn9ayy6 361 71 microtubule microtubule NN cord-001249-awn9ayy6 361 72 - - HYPH cord-001249-awn9ayy6 361 73 independent independent JJ cord-001249-awn9ayy6 361 74 steps step NNS cord-001249-awn9ayy6 361 75 in in IN cord-001249-awn9ayy6 361 76 Crimean Crimean NNP cord-001249-awn9ayy6 361 77 - - HYPH cord-001249-awn9ayy6 361 78 Congo Congo NNP cord-001249-awn9ayy6 361 79 hemorrhagic hemorrhagic JJ cord-001249-awn9ayy6 361 80 fever fever NN cord-001249-awn9ayy6 361 81 virus virus NN cord-001249-awn9ayy6 361 82 replication replication NN cord-001249-awn9ayy6 361 83 cycle cycle NN cord-001249-awn9ayy6 362 1 The the DT cord-001249-awn9ayy6 362 2 unique unique JJ cord-001249-awn9ayy6 362 3 architecture architecture NN cord-001249-awn9ayy6 362 4 of of IN cord-001249-awn9ayy6 362 5 Bunyamwera Bunyamwera NNP cord-001249-awn9ayy6 362 6 virus virus NN cord-001249-awn9ayy6 362 7 factories factory NNS cord-001249-awn9ayy6 362 8 around around IN cord-001249-awn9ayy6 362 9 the the DT cord-001249-awn9ayy6 362 10 Golgi Golgi NNP cord-001249-awn9ayy6 362 11 complex complex JJ cord-001249-awn9ayy6 362 12 Polymorphism polymorphism NN cord-001249-awn9ayy6 362 13 and and CC cord-001249-awn9ayy6 362 14 structural structural JJ cord-001249-awn9ayy6 362 15 maturation maturation NN cord-001249-awn9ayy6 362 16 of of IN cord-001249-awn9ayy6 362 17 bunyamwera bunyamwera NNP cord-001249-awn9ayy6 362 18 virus virus NN cord-001249-awn9ayy6 362 19 in in IN cord-001249-awn9ayy6 362 20 Golgi Golgi NNP cord-001249-awn9ayy6 362 21 and and CC cord-001249-awn9ayy6 362 22 post post JJ cord-001249-awn9ayy6 362 23 - - JJ cord-001249-awn9ayy6 362 24 Golgi golgi JJ cord-001249-awn9ayy6 362 25 compartments compartment NNS cord-001249-awn9ayy6 362 26 Key key JJ cord-001249-awn9ayy6 362 27 Golgi Golgi NNP cord-001249-awn9ayy6 362 28 factors factor NNS cord-001249-awn9ayy6 362 29 for for IN cord-001249-awn9ayy6 362 30 structural structural JJ cord-001249-awn9ayy6 362 31 and and CC cord-001249-awn9ayy6 362 32 functional functional JJ cord-001249-awn9ayy6 362 33 maturation maturation NN cord-001249-awn9ayy6 362 34 of of IN cord-001249-awn9ayy6 362 35 bunyamwera bunyamwera NN cord-001249-awn9ayy6 362 36 virus virus NN cord-001249-awn9ayy6 362 37 Immunocytochemical Immunocytochemical NNP cord-001249-awn9ayy6 362 38 analysis analysis NN cord-001249-awn9ayy6 362 39 of of IN cord-001249-awn9ayy6 362 40 Uukuniemi Uukuniemi NNP cord-001249-awn9ayy6 362 41 virus virus NN cord-001249-awn9ayy6 362 42 budding bud VBG cord-001249-awn9ayy6 362 43 compartments compartment NNS cord-001249-awn9ayy6 362 44 : : : cord-001249-awn9ayy6 362 45 role role NN cord-001249-awn9ayy6 362 46 of of IN cord-001249-awn9ayy6 362 47 the the DT cord-001249-awn9ayy6 362 48 intermediate intermediate JJ cord-001249-awn9ayy6 362 49 compartment compartment NN cord-001249-awn9ayy6 362 50 and and CC cord-001249-awn9ayy6 362 51 the the DT cord-001249-awn9ayy6 362 52 Golgi Golgi NNP cord-001249-awn9ayy6 362 53 stack stack NN cord-001249-awn9ayy6 362 54 in in IN cord-001249-awn9ayy6 362 55 virus virus NN cord-001249-awn9ayy6 362 56 maturation maturation NN cord-001249-awn9ayy6 362 57 Bunyavirus Bunyavirus NNP cord-001249-awn9ayy6 362 58 protein protein NN cord-001249-awn9ayy6 362 59 transport transport NN cord-001249-awn9ayy6 362 60 and and CC cord-001249-awn9ayy6 362 61 assembly assembly NN cord-001249-awn9ayy6 362 62 New New NNP cord-001249-awn9ayy6 362 63 and and CC cord-001249-awn9ayy6 362 64 Old Old NNP cord-001249-awn9ayy6 362 65 World World NNP cord-001249-awn9ayy6 362 66 hantaviruses hantaviruse NNS cord-001249-awn9ayy6 362 67 differentially differentially RB cord-001249-awn9ayy6 362 68 utilize utilize VBP cord-001249-awn9ayy6 362 69 host host NN cord-001249-awn9ayy6 362 70 cytoskeletal cytoskeletal JJ cord-001249-awn9ayy6 362 71 components component NNS cord-001249-awn9ayy6 362 72 during during IN cord-001249-awn9ayy6 362 73 their -PRON- PRP$ cord-001249-awn9ayy6 362 74 life life NN cord-001249-awn9ayy6 362 75 cycles cycle NNS cord-001249-awn9ayy6 362 76 Markers marker NNS cord-001249-awn9ayy6 362 77 for for IN cord-001249-awn9ayy6 362 78 trans trans NNP cord-001249-awn9ayy6 362 79 - - JJ cord-001249-awn9ayy6 362 80 Golgi Golgi NNP cord-001249-awn9ayy6 362 81 membranes membrane NNS cord-001249-awn9ayy6 362 82 and and CC cord-001249-awn9ayy6 362 83 the the DT cord-001249-awn9ayy6 362 84 intermediate intermediate JJ cord-001249-awn9ayy6 362 85 compartment compartment NN cord-001249-awn9ayy6 362 86 localize localize VBP cord-001249-awn9ayy6 362 87 to to IN cord-001249-awn9ayy6 362 88 induced induce VBN cord-001249-awn9ayy6 362 89 membranes membrane NNS cord-001249-awn9ayy6 362 90 with with IN cord-001249-awn9ayy6 362 91 distinct distinct JJ cord-001249-awn9ayy6 362 92 replication replication NN cord-001249-awn9ayy6 362 93 functions function NNS cord-001249-awn9ayy6 362 94 in in IN cord-001249-awn9ayy6 362 95 flavivirus flavivirus JJ cord-001249-awn9ayy6 362 96 - - HYPH cord-001249-awn9ayy6 362 97 infected infect VBN cord-001249-awn9ayy6 362 98 cells cell NNS cord-001249-awn9ayy6 362 99 Assembly assembly NN cord-001249-awn9ayy6 362 100 and and CC cord-001249-awn9ayy6 362 101 maturation maturation NN cord-001249-awn9ayy6 362 102 of of IN cord-001249-awn9ayy6 362 103 the the DT cord-001249-awn9ayy6 362 104 flavivirus flavivirus JJ cord-001249-awn9ayy6 362 105 Kunjin Kunjin NNP cord-001249-awn9ayy6 362 106 virus virus NN cord-001249-awn9ayy6 362 107 appear appear VBP cord-001249-awn9ayy6 362 108 to to TO cord-001249-awn9ayy6 362 109 occur occur VB cord-001249-awn9ayy6 362 110 in in IN cord-001249-awn9ayy6 362 111 the the DT cord-001249-awn9ayy6 362 112 rough rough JJ cord-001249-awn9ayy6 362 113 endoplasmic endoplasmic NN cord-001249-awn9ayy6 362 114 reticulum reticulum NN cord-001249-awn9ayy6 362 115 and and CC cord-001249-awn9ayy6 362 116 along along IN cord-001249-awn9ayy6 362 117 the the DT cord-001249-awn9ayy6 362 118 secretory secretory JJ cord-001249-awn9ayy6 362 119 pathway pathway NN cord-001249-awn9ayy6 362 120 , , , cord-001249-awn9ayy6 362 121 respectively respectively RB cord-001249-awn9ayy6 362 122 Endothelial endothelial JJ cord-001249-awn9ayy6 362 123 cell cell NN cord-001249-awn9ayy6 362 124 surface surface NN cord-001249-awn9ayy6 362 125 expression expression NN cord-001249-awn9ayy6 362 126 of of IN cord-001249-awn9ayy6 363 1 protein protein NN cord-001249-awn9ayy6 363 2 disulfide disulfide NNP cord-001249-awn9ayy6 363 3 isomerase isomerase NN cord-001249-awn9ayy6 363 4 activates activate VBZ cord-001249-awn9ayy6 363 5 beta1 beta1 NN cord-001249-awn9ayy6 363 6 and and CC cord-001249-awn9ayy6 363 7 beta3 beta3 NN cord-001249-awn9ayy6 363 8 integrins integrin NNS cord-001249-awn9ayy6 363 9 and and CC cord-001249-awn9ayy6 363 10 facilitates facilitate VBZ cord-001249-awn9ayy6 363 11 dengue dengue NN cord-001249-awn9ayy6 363 12 virus virus NN cord-001249-awn9ayy6 363 13 infection infection NN cord-001249-awn9ayy6 364 1 The the DT cord-001249-awn9ayy6 364 2 subcellular subcellular JJ cord-001249-awn9ayy6 364 3 distribution distribution NN cord-001249-awn9ayy6 364 4 of of IN cord-001249-awn9ayy6 364 5 multigene multigene JJ cord-001249-awn9ayy6 364 6 family family NN cord-001249-awn9ayy6 364 7 110 110 CD cord-001249-awn9ayy6 364 8 proteins protein NNS cord-001249-awn9ayy6 364 9 of of IN cord-001249-awn9ayy6 364 10 African african JJ cord-001249-awn9ayy6 364 11 swine swine NN cord-001249-awn9ayy6 364 12 fever fever NN cord-001249-awn9ayy6 364 13 virus virus NN cord-001249-awn9ayy6 364 14 is be VBZ cord-001249-awn9ayy6 364 15 determined determine VBN cord-001249-awn9ayy6 364 16 by by IN cord-001249-awn9ayy6 364 17 differences difference NNS cord-001249-awn9ayy6 364 18 in in IN cord-001249-awn9ayy6 364 19 C c NN cord-001249-awn9ayy6 364 20 - - HYPH cord-001249-awn9ayy6 364 21 terminal terminal NN cord-001249-awn9ayy6 364 22 KDEL KDEL NNP cord-001249-awn9ayy6 364 23 endoplasmic endoplasmic NN cord-001249-awn9ayy6 364 24 reticulum reticulum NN cord-001249-awn9ayy6 364 25 retention retention NN cord-001249-awn9ayy6 364 26 motifs motif NNS cord-001249-awn9ayy6 365 1 African african JJ cord-001249-awn9ayy6 365 2 swine swine NN cord-001249-awn9ayy6 365 3 fever fever NN cord-001249-awn9ayy6 365 4 virus virus NN cord-001249-awn9ayy6 365 5 is be VBZ cord-001249-awn9ayy6 365 6 enveloped envelop VBN cord-001249-awn9ayy6 365 7 by by IN cord-001249-awn9ayy6 365 8 a a DT cord-001249-awn9ayy6 365 9 two two CD cord-001249-awn9ayy6 365 10 - - HYPH cord-001249-awn9ayy6 365 11 membraned membraned NN cord-001249-awn9ayy6 365 12 collapsed collapsed JJ cord-001249-awn9ayy6 365 13 cisterna cisterna NNP cord-001249-awn9ayy6 365 14 derived derive VBN cord-001249-awn9ayy6 365 15 from from IN cord-001249-awn9ayy6 365 16 the the DT cord-001249-awn9ayy6 365 17 endoplasmic endoplasmic NN cord-001249-awn9ayy6 365 18 reticulum reticulum NN cord-001249-awn9ayy6 365 19 Viral Viral NNP cord-001249-awn9ayy6 365 20 reorganization reorganization NN cord-001249-awn9ayy6 365 21 of of IN cord-001249-awn9ayy6 365 22 the the DT cord-001249-awn9ayy6 365 23 secretory secretory JJ cord-001249-awn9ayy6 365 24 pathway pathway NN cord-001249-awn9ayy6 365 25 generates generate VBZ cord-001249-awn9ayy6 365 26 distinct distinct JJ cord-001249-awn9ayy6 365 27 organelles organelle NNS cord-001249-awn9ayy6 365 28 for for IN cord-001249-awn9ayy6 365 29 RNA RNA NNP cord-001249-awn9ayy6 365 30 replication replication NN cord-001249-awn9ayy6 365 31 Composition composition NN cord-001249-awn9ayy6 365 32 and and CC cord-001249-awn9ayy6 365 33 three three CD cord-001249-awn9ayy6 365 34 - - HYPH cord-001249-awn9ayy6 365 35 dimensional dimensional JJ cord-001249-awn9ayy6 365 36 architecture architecture NN cord-001249-awn9ayy6 365 37 of of IN cord-001249-awn9ayy6 365 38 the the DT cord-001249-awn9ayy6 365 39 dengue dengue NN cord-001249-awn9ayy6 365 40 virus virus NN cord-001249-awn9ayy6 365 41 replication replication NN cord-001249-awn9ayy6 365 42 and and CC cord-001249-awn9ayy6 365 43 assembly assembly NN cord-001249-awn9ayy6 365 44 sites site NNS cord-001249-awn9ayy6 366 1 African african JJ cord-001249-awn9ayy6 366 2 swine swine NN cord-001249-awn9ayy6 366 3 fever fever NN cord-001249-awn9ayy6 366 4 : : : cord-001249-awn9ayy6 366 5 morphopathology morphopathology NN cord-001249-awn9ayy6 366 6 of of IN cord-001249-awn9ayy6 366 7 a a DT cord-001249-awn9ayy6 366 8 viral viral JJ cord-001249-awn9ayy6 366 9 haemorrhagic haemorrhagic JJ cord-001249-awn9ayy6 366 10 disease disease NN cord-001249-awn9ayy6 366 11 Interaction Interaction NNP cord-001249-awn9ayy6 366 12 and and CC cord-001249-awn9ayy6 366 13 functional functional NNP cord-001249-awn9ayy6 366 14 association association NNP cord-001249-awn9ayy6 366 15 of of IN cord-001249-awn9ayy6 366 16 protein protein NNP cord-001249-awn9ayy6 366 17 disulfide disulfide NNP cord-001249-awn9ayy6 366 18 isomerase isomerase NN cord-001249-awn9ayy6 366 19 with with IN cord-001249-awn9ayy6 366 20 alphaVbeta3 alphavbeta3 NN cord-001249-awn9ayy6 366 21 integrin integrin NN cord-001249-awn9ayy6 366 22 on on IN cord-001249-awn9ayy6 366 23 endothelial endothelial JJ cord-001249-awn9ayy6 366 24 cells cell NNS cord-001249-awn9ayy6 367 1 Extracellular Extracellular NNP cord-001249-awn9ayy6 367 2 protein protein NN cord-001249-awn9ayy6 368 1 disulfide disulfide JJ cord-001249-awn9ayy6 368 2 isomerase isomerase NN cord-001249-awn9ayy6 368 3 regulates regulate VBZ cord-001249-awn9ayy6 368 4 coagulation coagulation NN cord-001249-awn9ayy6 368 5 on on IN cord-001249-awn9ayy6 368 6 endothelial endothelial JJ cord-001249-awn9ayy6 368 7 cells cell NNS cord-001249-awn9ayy6 368 8 through through IN cord-001249-awn9ayy6 368 9 modulation modulation NN cord-001249-awn9ayy6 368 10 of of IN cord-001249-awn9ayy6 368 11 phosphatidylserine phosphatidylserine NN cord-001249-awn9ayy6 368 12 exposure exposure NN cord-001249-awn9ayy6 368 13 Analysis Analysis NNP cord-001249-awn9ayy6 368 14 of of IN cord-001249-awn9ayy6 368 15 N N NNP cord-001249-awn9ayy6 368 16 - - HYPH cord-001249-awn9ayy6 368 17 linked link VBN cord-001249-awn9ayy6 368 18 glycosylation glycosylation NN cord-001249-awn9ayy6 368 19 of of IN cord-001249-awn9ayy6 368 20 hantaan hantaan JJ cord-001249-awn9ayy6 368 21 virus virus NN cord-001249-awn9ayy6 368 22 glycoproteins glycoprotein NNS cord-001249-awn9ayy6 368 23 and and CC cord-001249-awn9ayy6 368 24 the the DT cord-001249-awn9ayy6 368 25 role role NN cord-001249-awn9ayy6 368 26 of of IN cord-001249-awn9ayy6 368 27 oligosaccharide oligosaccharide NN cord-001249-awn9ayy6 368 28 side side NN cord-001249-awn9ayy6 368 29 chains chain NNS cord-001249-awn9ayy6 368 30 in in IN cord-001249-awn9ayy6 368 31 protein protein NN cord-001249-awn9ayy6 368 32 folding folding NN cord-001249-awn9ayy6 368 33 and and CC cord-001249-awn9ayy6 368 34 intracellular intracellular JJ cord-001249-awn9ayy6 368 35 trafficking traffic VBG cord-001249-awn9ayy6 368 36 Transient Transient NNP cord-001249-awn9ayy6 368 37 association association NN cord-001249-awn9ayy6 368 38 of of IN cord-001249-awn9ayy6 368 39 calnexin calnexin NNP cord-001249-awn9ayy6 368 40 and and CC cord-001249-awn9ayy6 368 41 calreticulin calreticulin NNP cord-001249-awn9ayy6 368 42 with with IN cord-001249-awn9ayy6 368 43 newly newly RB cord-001249-awn9ayy6 368 44 synthesized synthesize VBN cord-001249-awn9ayy6 368 45 G1 G1 NNP cord-001249-awn9ayy6 368 46 and and CC cord-001249-awn9ayy6 368 47 G2 G2 NNP cord-001249-awn9ayy6 368 48 glycoproteins glycoprotein NNS cord-001249-awn9ayy6 368 49 of of IN cord-001249-awn9ayy6 368 50 uukuniemi uukuniemi JJ cord-001249-awn9ayy6 368 51 virus virus NN cord-001249-awn9ayy6 368 52 ( ( -LRB- cord-001249-awn9ayy6 368 53 family family NN cord-001249-awn9ayy6 368 54 Bunyaviridae Bunyaviridae NNP cord-001249-awn9ayy6 368 55 ) ) -RRB- cord-001249-awn9ayy6 368 56 Formation formation NN cord-001249-awn9ayy6 368 57 and and CC cord-001249-awn9ayy6 368 58 intracellular intracellular JJ cord-001249-awn9ayy6 368 59 transport transport NN cord-001249-awn9ayy6 368 60 of of IN cord-001249-awn9ayy6 368 61 a a DT cord-001249-awn9ayy6 368 62 heterodimeric heterodimeric JJ cord-001249-awn9ayy6 368 63 viral viral JJ cord-001249-awn9ayy6 368 64 spike spike NN cord-001249-awn9ayy6 368 65 protein protein NN cord-001249-awn9ayy6 368 66 complex complex JJ cord-001249-awn9ayy6 368 67 Crimean Crimean NNP cord-001249-awn9ayy6 368 68 - - HYPH cord-001249-awn9ayy6 368 69 Congo Congo NNP cord-001249-awn9ayy6 368 70 hemorrhagic hemorrhagic JJ cord-001249-awn9ayy6 368 71 fever fever NN cord-001249-awn9ayy6 368 72 virus virus NN cord-001249-awn9ayy6 368 73 utilizes utilize VBZ cord-001249-awn9ayy6 368 74 a a DT cord-001249-awn9ayy6 368 75 clathrin clathrin NN cord-001249-awn9ayy6 368 76 - - : cord-001249-awn9ayy6 368 77 and and CC cord-001249-awn9ayy6 369 1 early early JJ cord-001249-awn9ayy6 369 2 endosome endosome NN cord-001249-awn9ayy6 369 3 - - HYPH cord-001249-awn9ayy6 369 4 dependent dependent JJ cord-001249-awn9ayy6 369 5 entry entry NN cord-001249-awn9ayy6 369 6 pathway pathway NN cord-001249-awn9ayy6 369 7 Sensors sensor NNS cord-001249-awn9ayy6 369 8 and and CC cord-001249-awn9ayy6 369 9 regulators regulator NNS cord-001249-awn9ayy6 369 10 of of IN cord-001249-awn9ayy6 369 11 intracellular intracellular JJ cord-001249-awn9ayy6 369 12 pH ph NN cord-001249-awn9ayy6 369 13 Overexpression Overexpression NNP cord-001249-awn9ayy6 369 14 of of IN cord-001249-awn9ayy6 369 15 thiol thiol NN cord-001249-awn9ayy6 369 16 / / SYM cord-001249-awn9ayy6 369 17 disulfide disulfide NN cord-001249-awn9ayy6 369 18 isomerases isomerases NNP cord-001249-awn9ayy6 369 19 enhances enhance VBZ cord-001249-awn9ayy6 369 20 membrane membrane NN cord-001249-awn9ayy6 369 21 fusion fusion NN cord-001249-awn9ayy6 369 22 directed direct VBN cord-001249-awn9ayy6 369 23 by by IN cord-001249-awn9ayy6 369 24 the the DT cord-001249-awn9ayy6 369 25 Newcastle Newcastle NNP cord-001249-awn9ayy6 369 26 disease disease NN cord-001249-awn9ayy6 369 27 virus virus NN cord-001249-awn9ayy6 369 28 fusion fusion NN cord-001249-awn9ayy6 369 29 protein protein NN cord-001249-awn9ayy6 369 30 Thiol Thiol NNP cord-001249-awn9ayy6 369 31 / / SYM cord-001249-awn9ayy6 369 32 disulfide disulfide NN cord-001249-awn9ayy6 369 33 exchange exchange NN cord-001249-awn9ayy6 369 34 is be VBZ cord-001249-awn9ayy6 369 35 required require VBN cord-001249-awn9ayy6 369 36 for for IN cord-001249-awn9ayy6 369 37 membrane membrane NN cord-001249-awn9ayy6 369 38 fusion fusion NN cord-001249-awn9ayy6 369 39 directed direct VBN cord-001249-awn9ayy6 369 40 by by IN cord-001249-awn9ayy6 369 41 the the DT cord-001249-awn9ayy6 369 42 Newcastle Newcastle NNP cord-001249-awn9ayy6 369 43 disease disease NN cord-001249-awn9ayy6 369 44 virus virus NN cord-001249-awn9ayy6 369 45 fusion fusion NN cord-001249-awn9ayy6 369 46 protein protein NN cord-001249-awn9ayy6 369 47 Role role NN cord-001249-awn9ayy6 369 48 of of IN cord-001249-awn9ayy6 369 49 thiol thiol NN cord-001249-awn9ayy6 369 50 / / SYM cord-001249-awn9ayy6 369 51 disulfide disulfide NN cord-001249-awn9ayy6 369 52 exchange exchange NNP cord-001249-awn9ayy6 369 53 in in IN cord-001249-awn9ayy6 369 54 newcastle newcastle NNP cord-001249-awn9ayy6 369 55 disease disease NNP cord-001249-awn9ayy6 369 56 virus virus NN cord-001249-awn9ayy6 369 57 entry entry NN cord-001249-awn9ayy6 369 58 Galectin-9 Galectin-9 NNP cord-001249-awn9ayy6 369 59 binding bind VBG cord-001249-awn9ayy6 369 60 to to IN cord-001249-awn9ayy6 369 61 cell cell NN cord-001249-awn9ayy6 369 62 surface surface NN cord-001249-awn9ayy6 369 63 protein protein NN cord-001249-awn9ayy6 370 1 disulfide disulfide JJ cord-001249-awn9ayy6 370 2 isomerase isomerase NN cord-001249-awn9ayy6 370 3 regulates regulate VBZ cord-001249-awn9ayy6 370 4 the the DT cord-001249-awn9ayy6 370 5 redox redox JJ cord-001249-awn9ayy6 370 6 environment environment NN cord-001249-awn9ayy6 370 7 to to TO cord-001249-awn9ayy6 370 8 enhance enhance VB cord-001249-awn9ayy6 370 9 T t NN cord-001249-awn9ayy6 370 10 - - HYPH cord-001249-awn9ayy6 370 11 cell cell NN cord-001249-awn9ayy6 370 12 migration migration NN cord-001249-awn9ayy6 370 13 and and CC cord-001249-awn9ayy6 370 14 HIV HIV NNP cord-001249-awn9ayy6 370 15 entry entry NN cord-001249-awn9ayy6 370 16 Thiol Thiol NNP cord-001249-awn9ayy6 370 17 / / SYM cord-001249-awn9ayy6 370 18 disulfide disulfide NN cord-001249-awn9ayy6 370 19 exchange exchange NN cord-001249-awn9ayy6 371 1 is be VBZ cord-001249-awn9ayy6 371 2 a a DT cord-001249-awn9ayy6 371 3 prerequisite prerequisite NN cord-001249-awn9ayy6 371 4 for for IN cord-001249-awn9ayy6 371 5 CXCR4-tropic CXCR4-tropic NNP cord-001249-awn9ayy6 371 6 HIV-1 HIV-1 NNP cord-001249-awn9ayy6 371 7 envelopemediated envelopemediate VBD cord-001249-awn9ayy6 371 8 T T NNP cord-001249-awn9ayy6 371 9 - - HYPH cord-001249-awn9ayy6 371 10 cell cell NN cord-001249-awn9ayy6 371 11 fusion fusion NN cord-001249-awn9ayy6 371 12 during during IN cord-001249-awn9ayy6 371 13 viral viral JJ cord-001249-awn9ayy6 371 14 entry entry NN cord-001249-awn9ayy6 372 1 The the DT cord-001249-awn9ayy6 372 2 catalytic catalytic JJ cord-001249-awn9ayy6 372 3 activity activity NN cord-001249-awn9ayy6 372 4 of of IN cord-001249-awn9ayy6 372 5 protein protein NN cord-001249-awn9ayy6 372 6 disulfide disulfide NN cord-001249-awn9ayy6 372 7 isomerase isomerase NN cord-001249-awn9ayy6 372 8 is be VBZ cord-001249-awn9ayy6 372 9 involved involve VBN cord-001249-awn9ayy6 372 10 in in IN cord-001249-awn9ayy6 372 11 human human JJ cord-001249-awn9ayy6 372 12 immunodeficiency immunodeficiency NN cord-001249-awn9ayy6 372 13 virus virus NN cord-001249-awn9ayy6 372 14 envelope envelope NN cord-001249-awn9ayy6 372 15 - - HYPH cord-001249-awn9ayy6 372 16 mediated mediate VBN cord-001249-awn9ayy6 372 17 membrane membrane NN cord-001249-awn9ayy6 372 18 fusion fusion NN cord-001249-awn9ayy6 372 19 after after IN cord-001249-awn9ayy6 372 20 CD4 CD4 NNP cord-001249-awn9ayy6 372 21 cell cell NN cord-001249-awn9ayy6 372 22 binding bind VBG cord-001249-awn9ayy6 372 23 Inactivation Inactivation NNP cord-001249-awn9ayy6 372 24 of of IN cord-001249-awn9ayy6 372 25 hantaviruses hantaviruses NNP cord-001249-awn9ayy6 372 26 by by IN cord-001249-awn9ayy6 372 27 N N NNP cord-001249-awn9ayy6 372 28 - - HYPH cord-001249-awn9ayy6 372 29 ethylmaleimide ethylmaleimide NNP cord-001249-awn9ayy6 372 30 preserves preserve VBZ cord-001249-awn9ayy6 372 31 virion virion NN cord-001249-awn9ayy6 372 32 integrity integrity NN cord-001249-awn9ayy6 372 33 Extracellular Extracellular NNP cord-001249-awn9ayy6 372 34 disulfide disulfide JJ cord-001249-awn9ayy6 372 35 exchange exchange NN cord-001249-awn9ayy6 372 36 and and CC cord-001249-awn9ayy6 372 37 the the DT cord-001249-awn9ayy6 372 38 regulation regulation NN cord-001249-awn9ayy6 372 39 of of IN cord-001249-awn9ayy6 372 40 cellular cellular JJ cord-001249-awn9ayy6 372 41 function function NN cord-001249-awn9ayy6 372 42 Role role NN cord-001249-awn9ayy6 372 43 of of IN cord-001249-awn9ayy6 372 44 PDI PDI NNP cord-001249-awn9ayy6 372 45 in in IN cord-001249-awn9ayy6 372 46 regulating regulate VBG cord-001249-awn9ayy6 372 47 tissue tissue NN cord-001249-awn9ayy6 372 48 factor factor NN cord-001249-awn9ayy6 372 49 : : : cord-001249-awn9ayy6 372 50 FVIIa FVIIa NNS cord-001249-awn9ayy6 372 51 activity activity NN cord-001249-awn9ayy6 372 52 Sustained sustain VBN cord-001249-awn9ayy6 373 1 integrin integrin NNP cord-001249-awn9ayy6 373 2 ligation ligation NN cord-001249-awn9ayy6 373 3 involves involve VBZ cord-001249-awn9ayy6 373 4 extracellular extracellular JJ cord-001249-awn9ayy6 373 5 free free JJ cord-001249-awn9ayy6 373 6 sulfhydryls sulfhydryls NN cord-001249-awn9ayy6 373 7 and and CC cord-001249-awn9ayy6 373 8 enzymatically enzymatically RB cord-001249-awn9ayy6 373 9 catalyzed catalyze VBN cord-001249-awn9ayy6 374 1 disulfide disulfide NNP cord-001249-awn9ayy6 374 2 exchange exchange NNP cord-001249-awn9ayy6 375 1 The the DT cord-001249-awn9ayy6 375 2 molecular molecular JJ cord-001249-awn9ayy6 375 3 basis basis NN cord-001249-awn9ayy6 375 4 of of IN cord-001249-awn9ayy6 375 5 leukocyte leukocyte NN cord-001249-awn9ayy6 375 6 recruitment recruitment NN cord-001249-awn9ayy6 375 7 and and CC cord-001249-awn9ayy6 375 8 its -PRON- PRP$ cord-001249-awn9ayy6 375 9 deficiencies deficiency NNS cord-001249-awn9ayy6 375 10 Viral viral JJ cord-001249-awn9ayy6 375 11 haemorrhagic haemorrhagic JJ cord-001249-awn9ayy6 375 12 fever fever NN cord-001249-awn9ayy6 375 13 and and CC cord-001249-awn9ayy6 375 14 vascular vascular JJ cord-001249-awn9ayy6 375 15 alterations alteration NNS cord-001249-awn9ayy6 376 1 Protein protein NN cord-001249-awn9ayy6 376 2 disulfide disulfide NN cord-001249-awn9ayy6 376 3 isomerase isomerase NN cord-001249-awn9ayy6 376 4 acts act VBZ cord-001249-awn9ayy6 376 5 as as IN cord-001249-awn9ayy6 376 6 an an DT cord-001249-awn9ayy6 376 7 injury injury NN cord-001249-awn9ayy6 376 8 response response NN cord-001249-awn9ayy6 376 9 signal signal NN cord-001249-awn9ayy6 376 10 that that WDT cord-001249-awn9ayy6 376 11 enhances enhance VBZ cord-001249-awn9ayy6 376 12 fibrin fibrin NN cord-001249-awn9ayy6 376 13 generation generation NN cord-001249-awn9ayy6 376 14 via via IN cord-001249-awn9ayy6 376 15 tissue tissue NN cord-001249-awn9ayy6 376 16 factor factor NN cord-001249-awn9ayy6 376 17 activation activation NN cord-001249-awn9ayy6 377 1 The the DT cord-001249-awn9ayy6 377 2 interactions interaction NNS cord-001249-awn9ayy6 377 3 between between IN cord-001249-awn9ayy6 377 4 inflammation inflammation NN cord-001249-awn9ayy6 377 5 and and CC cord-001249-awn9ayy6 377 6 coagulation coagulation NN cord-001249-awn9ayy6 377 7 Disseminated disseminate VBD cord-001249-awn9ayy6 377 8 intravascular intravascular JJ cord-001249-awn9ayy6 377 9 coagulation coagulation NN cord-001249-awn9ayy6 377 10 : : : cord-001249-awn9ayy6 377 11 a a DT cord-001249-awn9ayy6 377 12 review review NN cord-001249-awn9ayy6 377 13 for for IN cord-001249-awn9ayy6 377 14 the the DT cord-001249-awn9ayy6 377 15 internist internist NN cord-001249-awn9ayy6 378 1 The the DT cord-001249-awn9ayy6 378 2 tissue tissue NN cord-001249-awn9ayy6 378 3 factor factor NN cord-001249-awn9ayy6 378 4 pathway pathway NN cord-001249-awn9ayy6 378 5 in in IN cord-001249-awn9ayy6 378 6 disseminated disseminate VBN cord-001249-awn9ayy6 378 7 intravascular intravascular JJ cord-001249-awn9ayy6 378 8 coagulation coagulation NN cord-001249-awn9ayy6 378 9 Disseminated disseminate VBD cord-001249-awn9ayy6 378 10 intravascular intravascular JJ cord-001249-awn9ayy6 378 11 coagulation coagulation NN cord-001249-awn9ayy6 378 12 ( ( -LRB- cord-001249-awn9ayy6 378 13 DIC DIC NNP cord-001249-awn9ayy6 378 14 ) ) -RRB- cord-001249-awn9ayy6 378 15 in in IN cord-001249-awn9ayy6 378 16 rabbit rabbit NN cord-001249-awn9ayy6 378 17 haemorrhagic haemorrhagic JJ cord-001249-awn9ayy6 378 18 disease disease NN cord-001249-awn9ayy6 378 19 Cytokine cytokine NN cord-001249-awn9ayy6 378 20 levels level NNS cord-001249-awn9ayy6 378 21 in in IN cord-001249-awn9ayy6 378 22 Crimean Crimean NNP cord-001249-awn9ayy6 378 23 - - HYPH cord-001249-awn9ayy6 378 24 Congo Congo NNP cord-001249-awn9ayy6 378 25 hemorrhagic hemorrhagic JJ cord-001249-awn9ayy6 378 26 fever fever NN cord-001249-awn9ayy6 378 27 Redox redox NN cord-001249-awn9ayy6 378 28 - - HYPH cord-001249-awn9ayy6 378 29 regulated regulate VBN cord-001249-awn9ayy6 378 30 peptide peptide NN cord-001249-awn9ayy6 378 31 transfer transfer NN cord-001249-awn9ayy6 378 32 from from IN cord-001249-awn9ayy6 378 33 the the DT cord-001249-awn9ayy6 378 34 transporter transporter NN cord-001249-awn9ayy6 378 35 associated associate VBN cord-001249-awn9ayy6 378 36 with with IN cord-001249-awn9ayy6 378 37 antigen antigen NN cord-001249-awn9ayy6 378 38 processing processing NN cord-001249-awn9ayy6 378 39 to to IN cord-001249-awn9ayy6 378 40 major major JJ cord-001249-awn9ayy6 378 41 histocompatibility histocompatibility NN cord-001249-awn9ayy6 378 42 complex complex JJ cord-001249-awn9ayy6 378 43 class class NN cord-001249-awn9ayy6 379 1 I -PRON- PRP cord-001249-awn9ayy6 379 2 molecules molecule NNS cord-001249-awn9ayy6 379 3 by by IN cord-001249-awn9ayy6 379 4 protein protein NN cord-001249-awn9ayy6 379 5 disulfide disulfide NN cord-001249-awn9ayy6 379 6 isomerase isomerase NN cord-001249-awn9ayy6 379 7 Functions function NNS cord-001249-awn9ayy6 379 8 of of IN cord-001249-awn9ayy6 379 9 ERp57 ERp57 NNS cord-001249-awn9ayy6 379 10 in in IN cord-001249-awn9ayy6 379 11 the the DT cord-001249-awn9ayy6 379 12 folding folding NN cord-001249-awn9ayy6 379 13 and and CC cord-001249-awn9ayy6 379 14 assembly assembly NN cord-001249-awn9ayy6 379 15 of of IN cord-001249-awn9ayy6 379 16 major major JJ cord-001249-awn9ayy6 379 17 histocompatibility histocompatibility NN cord-001249-awn9ayy6 379 18 complex complex JJ cord-001249-awn9ayy6 379 19 class class NN cord-001249-awn9ayy6 380 1 I -PRON- PRP cord-001249-awn9ayy6 380 2 molecules molecule NNS cord-001249-awn9ayy6 380 3 Running run VBG cord-001249-awn9ayy6 380 4 the the DT cord-001249-awn9ayy6 380 5 gauntlet gauntlet NN cord-001249-awn9ayy6 380 6 : : : cord-001249-awn9ayy6 381 1 from from IN cord-001249-awn9ayy6 381 2 peptide peptide JJ cord-001249-awn9ayy6 381 3 generation generation NN cord-001249-awn9ayy6 381 4 to to IN cord-001249-awn9ayy6 381 5 antigen antigen NN cord-001249-awn9ayy6 381 6 presentation presentation NN cord-001249-awn9ayy6 381 7 by by IN cord-001249-awn9ayy6 381 8 MHC MHC NNP cord-001249-awn9ayy6 381 9 class class NN cord-001249-awn9ayy6 382 1 I -PRON- PRP cord-001249-awn9ayy6 382 2 Protein Protein NNP cord-001249-awn9ayy6 382 3 disulfide disulfide NN cord-001249-awn9ayy6 382 4 isomerase isomerase NN cord-001249-awn9ayy6 382 5 inhibitors inhibitor NNS cord-001249-awn9ayy6 382 6 constitute constitute VBP cord-001249-awn9ayy6 382 7 a a DT cord-001249-awn9ayy6 382 8 new new JJ cord-001249-awn9ayy6 382 9 class class NN cord-001249-awn9ayy6 382 10 of of IN cord-001249-awn9ayy6 382 11 antithrombotic antithrombotic JJ cord-001249-awn9ayy6 382 12 agents agent NNS cord-001249-awn9ayy6 382 13 Inhibitors Inhibitors NNP cord-001249-awn9ayy6 382 14 of of IN cord-001249-awn9ayy6 382 15 protein protein NN cord-001249-awn9ayy6 383 1 disulfide disulfide JJ cord-001249-awn9ayy6 383 2 isomerase isomerase NN cord-001249-awn9ayy6 383 3 suppress suppress VBP cord-001249-awn9ayy6 383 4 apoptosis apoptosis NN cord-001249-awn9ayy6 383 5 induced induce VBN cord-001249-awn9ayy6 383 6 by by IN cord-001249-awn9ayy6 383 7 misfolded misfolded JJ cord-001249-awn9ayy6 383 8 proteins protein NNS cord-001249-awn9ayy6 383 9 We -PRON- PRP cord-001249-awn9ayy6 383 10 would would MD cord-001249-awn9ayy6 383 11 like like VB cord-001249-awn9ayy6 383 12 to to TO cord-001249-awn9ayy6 383 13 thank thank VB cord-001249-awn9ayy6 383 14 Professor Professor NNP cord-001249-awn9ayy6 383 15 Robert Robert NNP cord-001249-awn9ayy6 383 16 B. B. NNP cord-001249-awn9ayy6 383 17 Tesh Tesh NNP cord-001249-awn9ayy6 383 18 and and CC cord-001249-awn9ayy6 383 19 Dr Dr NNP cord-001249-awn9ayy6 383 20 Piet Piet NNP cord-001249-awn9ayy6 383 21 van van NNP cord-001249-awn9ayy6 383 22 Rijn Rijn NNP cord-001249-awn9ayy6 383 23 for for IN cord-001249-awn9ayy6 383 24 the the DT cord-001249-awn9ayy6 383 25 gift gift NN cord-001249-awn9ayy6 383 26 of of IN cord-001249-awn9ayy6 383 27 the the DT cord-001249-awn9ayy6 383 28 GV GV NNP cord-001249-awn9ayy6 383 29 and and CC cord-001249-awn9ayy6 383 30 NSDV NSDV NNP cord-001249-awn9ayy6 383 31 isolates isolate VBZ cord-001249-awn9ayy6 383 32 ; ; : cord-001249-awn9ayy6 383 33 Dr Dr NNP cord-001249-awn9ayy6 383 34 Nathalie Nathalie NNP cord-001249-awn9ayy6 383 35 Vachiery Vachiery NNP cord-001249-awn9ayy6 383 36 for for IN cord-001249-awn9ayy6 383 37 the the DT cord-001249-awn9ayy6 383 38 CJE CJE NNP cord-001249-awn9ayy6 383 39 cells cell NNS cord-001249-awn9ayy6 383 40 ; ; : cord-001249-awn9ayy6 383 41 Professor Professor NNP cord-001249-awn9ayy6 383 42 Adolfo Adolfo NNP cord-001249-awn9ayy6 383 43 Garcia Garcia NNP cord-001249-awn9ayy6 383 44 - - HYPH cord-001249-awn9ayy6 383 45 Sastre Sastre NNP cord-001249-awn9ayy6 383 46 for for IN cord-001249-awn9ayy6 383 47 pCAGGS pCAGGS NNP cord-001249-awn9ayy6 383 48 - - HYPH cord-001249-awn9ayy6 383 49 MCSII MCSII NNP cord-001249-awn9ayy6 383 50 plasmid plasmid NN cord-001249-awn9ayy6 383 51 ; ; : cord-001249-awn9ayy6 383 52 Dr Dr NNP cord-001249-awn9ayy6 383 53 Philippa Philippa NNP cord-001249-awn9ayy6 383 54 Hawes Hawes NNP cord-001249-awn9ayy6 383 55 and and CC cord-001249-awn9ayy6 383 56 Dr Dr NNP cord-001249-awn9ayy6 383 57 Christopher Christopher NNP cord-001249-awn9ayy6 383 58 Netherton Netherton NNP cord-001249-awn9ayy6 383 59 of of IN cord-001249-awn9ayy6 383 60 this this DT cord-001249-awn9ayy6 383 61 institute institute NN cord-001249-awn9ayy6 383 62 for for IN cord-001249-awn9ayy6 383 63 antibodies antibody NNS cord-001249-awn9ayy6 383 64 against against IN cord-001249-awn9ayy6 383 65 GM130 GM130 NNP cord-001249-awn9ayy6 383 66 , , , cord-001249-awn9ayy6 383 67 p102 p102 NNS cord-001249-awn9ayy6 383 68 , , , cord-001249-awn9ayy6 383 69 ERGIC53 ergic53 NN cord-001249-awn9ayy6 383 70 , , , cord-001249-awn9ayy6 383 71 PDI PDI NNP cord-001249-awn9ayy6 383 72 ( ( -LRB- cord-001249-awn9ayy6 383 73 clone clone NN cord-001249-awn9ayy6 383 74 1D3 1d3 CD cord-001249-awn9ayy6 383 75 ) ) -RRB- cord-001249-awn9ayy6 383 76 and and CC cord-001249-awn9ayy6 383 77 ERp57 ERp57 NNS cord-001249-awn9ayy6 383 78 . . . cord-001249-awn9ayy6 383 79 _SP