id	sid	tid	token	lemma	pos
cord-001142-puj74k7y	1	1	key	key	NN
cord-001142-puj74k7y	1	2	:	:	:
cord-001142-puj74k7y	2	1	cord-001142-puj74k7y	cord-001142-puj74k7y	CD
cord-001142-puj74k7y	2	2	authors	author	NNS
cord-001142-puj74k7y	2	3	:	:	:
cord-001142-puj74k7y	2	4	Crescenzo	Crescenzo	NNP
cord-001142-puj74k7y	2	5	-	-	HYPH
cord-001142-puj74k7y	2	6	Chaigne	Chaigne	NNP
cord-001142-puj74k7y	2	7	,	,	,
cord-001142-puj74k7y	2	8	Bernadette	Bernadette	NNP
cord-001142-puj74k7y	2	9	;	;	:
cord-001142-puj74k7y	2	10	Barbezange	Barbezange	NNP
cord-001142-puj74k7y	2	11	,	,	,
cord-001142-puj74k7y	2	12	Cyril	Cyril	NNP
cord-001142-puj74k7y	2	13	;	;	:
cord-001142-puj74k7y	2	14	van	van	NNP
cord-001142-puj74k7y	2	15	der	der	NNP
cord-001142-puj74k7y	2	16	Werf	Werf	NNP
cord-001142-puj74k7y	2	17	,	,	,
cord-001142-puj74k7y	2	18	Sylvie	Sylvie	NNP
cord-001142-puj74k7y	2	19	title	title	NN
cord-001142-puj74k7y	2	20	:	:	:
cord-001142-puj74k7y	3	1	The	the	DT
cord-001142-puj74k7y	3	2	Panhandle	Panhandle	NNP
cord-001142-puj74k7y	3	3	Formed	form	VBN
cord-001142-puj74k7y	3	4	by	by	IN
cord-001142-puj74k7y	3	5	Influenza	Influenza	NNP
cord-001142-puj74k7y	3	6	A	A	NNP
cord-001142-puj74k7y	3	7	and	and	CC
cord-001142-puj74k7y	3	8	C	C	NNP
cord-001142-puj74k7y	3	9	Virus	virus	NN
cord-001142-puj74k7y	4	1	NS	NS	NNP
cord-001142-puj74k7y	4	2	Non	Non	NNP
cord-001142-puj74k7y	4	3	-	-	NNP
cord-001142-puj74k7y	4	4	Coding	coding	JJ
cord-001142-puj74k7y	4	5	Regions	Regions	NNPS
cord-001142-puj74k7y	4	6	Determines	determine	VBZ
cord-001142-puj74k7y	4	7	NS	NS	NNP
cord-001142-puj74k7y	4	8	Segment	Segment	NNP
cord-001142-puj74k7y	4	9	Expression	Expression	NNP
cord-001142-puj74k7y	4	10	date	date	NN
cord-001142-puj74k7y	4	11	:	:	:
cord-001142-puj74k7y	4	12	2013	2013	CD
cord-001142-puj74k7y	4	13	-	-	SYM
cord-001142-puj74k7y	4	14	11	11	CD
cord-001142-puj74k7y	4	15	-	-	SYM
cord-001142-puj74k7y	4	16	21	21	CD
cord-001142-puj74k7y	4	17	journal	journal	NN
cord-001142-puj74k7y	4	18	:	:	:
cord-001142-puj74k7y	5	1	PLoS	PLoS	NNP
cord-001142-puj74k7y	6	1	One	one	CD
cord-001142-puj74k7y	6	2	DOI	doi	NN
cord-001142-puj74k7y	6	3	:	:	:
cord-001142-puj74k7y	6	4	10.1371	10.1371	CD
cord-001142-puj74k7y	6	5	/	/	SYM
cord-001142-puj74k7y	6	6	journal.pone.0081550	journal.pone.0081550	NNP
cord-001142-puj74k7y	6	7	sha	sha	NNP
cord-001142-puj74k7y	6	8	:	:	:
cord-001142-puj74k7y	7	1	5c10b401cc688e4f966e2ab5f41ab3a056685a3d	5c10b401cc688e4f966e2ab5f41ab3a056685a3d	CD
cord-001142-puj74k7y	7	2	doc_id	doc_id	CD
cord-001142-puj74k7y	7	3	:	:	:
cord-001142-puj74k7y	7	4	1142	1142	CD
cord-001142-puj74k7y	7	5	cord_uid	cord_uid	NNS
cord-001142-puj74k7y	7	6	:	:	:
cord-001142-puj74k7y	8	1	puj74k7y	puj74k7y	NNP
cord-001142-puj74k7y	8	2	Exchange	Exchange	NNP
cord-001142-puj74k7y	8	3	of	of	IN
cord-001142-puj74k7y	8	4	the	the	DT
cord-001142-puj74k7y	8	5	extremities	extremity	NNS
cord-001142-puj74k7y	8	6	of	of	IN
cord-001142-puj74k7y	8	7	the	the	DT
cord-001142-puj74k7y	8	8	NS	NS	NNP
cord-001142-puj74k7y	8	9	segment	segment	NN
cord-001142-puj74k7y	8	10	of	of	IN
cord-001142-puj74k7y	8	11	type	type	NN
cord-001142-puj74k7y	8	12	A	a	NN
cord-001142-puj74k7y	8	13	and	and	CC
cord-001142-puj74k7y	8	14	C	c	NN
cord-001142-puj74k7y	8	15	influenza	influenza	NN
cord-001142-puj74k7y	8	16	viruses	virus	NNS
cord-001142-puj74k7y	8	17	in	in	IN
cord-001142-puj74k7y	8	18	reverse	reverse	JJ
cord-001142-puj74k7y	8	19	genetics	genetics	NN
cord-001142-puj74k7y	8	20	systems	system	NNS
cord-001142-puj74k7y	8	21	was	be	VBD
cord-001142-puj74k7y	8	22	used	use	VBN
cord-001142-puj74k7y	8	23	to	to	TO
cord-001142-puj74k7y	8	24	assess	assess	VB
cord-001142-puj74k7y	8	25	their	-PRON-	PRP$
cord-001142-puj74k7y	8	26	putative	putative	JJ
cord-001142-puj74k7y	8	27	role	role	NN
cord-001142-puj74k7y	8	28	in	in	IN
cord-001142-puj74k7y	8	29	type	type	NN
cord-001142-puj74k7y	8	30	specificity	specificity	NN
cord-001142-puj74k7y	8	31	.	.	.
cord-001142-puj74k7y	9	1	Restoration	restoration	NN
cord-001142-puj74k7y	9	2	of	of	IN
cord-001142-puj74k7y	9	3	each	each	DT
cord-001142-puj74k7y	9	4	specific	specific	JJ
cord-001142-puj74k7y	9	5	proximal	proximal	JJ
cord-001142-puj74k7y	9	6	panhandle	panhandle	NN
cord-001142-puj74k7y	9	7	was	be	VBD
cord-001142-puj74k7y	9	8	mandatory	mandatory	JJ
cord-001142-puj74k7y	9	9	to	to	TO
cord-001142-puj74k7y	9	10	allow	allow	VB
cord-001142-puj74k7y	9	11	the	the	DT
cord-001142-puj74k7y	9	12	rescue	rescue	NN
cord-001142-puj74k7y	9	13	of	of	IN
cord-001142-puj74k7y	9	14	viruses	virus	NNS
cord-001142-puj74k7y	9	15	with	with	IN
cord-001142-puj74k7y	9	16	heterotypic	heterotypic	NNP
cord-001142-puj74k7y	9	17	extremities	extremity	NNS
cord-001142-puj74k7y	9	18	.	.	.
cord-001142-puj74k7y	10	1	Moreover	moreover	RB
cord-001142-puj74k7y	10	2	,	,	,
cord-001142-puj74k7y	10	3	the	the	DT
cord-001142-puj74k7y	10	4	transcription	transcription	NN
cord-001142-puj74k7y	10	5	level	level	NN
cord-001142-puj74k7y	10	6	of	of	IN
cord-001142-puj74k7y	10	7	the	the	DT
cord-001142-puj74k7y	10	8	modified	modify	VBN
cord-001142-puj74k7y	10	9	segment	segment	NN
cord-001142-puj74k7y	10	10	seemed	seem	VBD
cord-001142-puj74k7y	10	11	to	to	TO
cord-001142-puj74k7y	10	12	be	be	VB
cord-001142-puj74k7y	10	13	directly	directly	RB
cord-001142-puj74k7y	10	14	affected	affect	VBN
cord-001142-puj74k7y	10	15	by	by	IN
cord-001142-puj74k7y	10	16	the	the	DT
cord-001142-puj74k7y	10	17	distal	distal	JJ
cord-001142-puj74k7y	10	18	panhandle	panhandle	NN
cord-001142-puj74k7y	10	19	strength	strength	NN
cord-001142-puj74k7y	10	20	.	.	.
cord-001142-puj74k7y	11	1	Influenza	Influenza	NNP
cord-001142-puj74k7y	11	2	A	A	NNP
cord-001142-puj74k7y	11	3	,	,	,
cord-001142-puj74k7y	11	4	B	b	NN
cord-001142-puj74k7y	11	5	and	and	CC
cord-001142-puj74k7y	11	6	C	c	NN
cord-001142-puj74k7y	11	7	viruses	virus	NNS
cord-001142-puj74k7y	11	8	are	be	VBP
cord-001142-puj74k7y	11	9	members	member	NNS
cord-001142-puj74k7y	11	10	of	of	IN
cord-001142-puj74k7y	11	11	the	the	DT
cord-001142-puj74k7y	11	12	Orthomyxoviridae	Orthomyxoviridae	NNP
cord-001142-puj74k7y	11	13	family	family	NN
cord-001142-puj74k7y	11	14	,	,	,
cord-001142-puj74k7y	11	15	a	a	DT
cord-001142-puj74k7y	11	16	group	group	NN
cord-001142-puj74k7y	11	17	of	of	IN
cord-001142-puj74k7y	11	18	enveloped	envelop	VBN
cord-001142-puj74k7y	11	19	,	,	,
cord-001142-puj74k7y	11	20	segmented	segmented	JJ
cord-001142-puj74k7y	11	21	,	,	,
cord-001142-puj74k7y	11	22	single	single	RB
cord-001142-puj74k7y	11	23	-	-	HYPH
cord-001142-puj74k7y	11	24	stranded	strand	VBN
cord-001142-puj74k7y	11	25	negative	negative	JJ
cord-001142-puj74k7y	11	26	-	-	HYPH
cord-001142-puj74k7y	11	27	sense	sense	NN
cord-001142-puj74k7y	11	28	RNA	RNA	NNP
cord-001142-puj74k7y	11	29	viruses	virus	NNS
cord-001142-puj74k7y	11	30	.	.	.
cord-001142-puj74k7y	12	1	Reassortment	Reassortment	NNP
cord-001142-puj74k7y	12	2	between	between	IN
cord-001142-puj74k7y	12	3	type	type	NN
cord-001142-puj74k7y	12	4	A	a	NN
cord-001142-puj74k7y	12	5	,	,	,
cord-001142-puj74k7y	12	6	B	B	NNP
cord-001142-puj74k7y	12	7	and	and	CC
cord-001142-puj74k7y	12	8	C	C	NNP
cord-001142-puj74k7y	12	9	influenza	influenza	NN
cord-001142-puj74k7y	12	10	viruses	virus	NNS
cord-001142-puj74k7y	12	11	has	have	VBZ
cord-001142-puj74k7y	12	12	never	never	RB
cord-001142-puj74k7y	12	13	been	be	VBN
cord-001142-puj74k7y	12	14	reported	report	VBN
cord-001142-puj74k7y	12	15	to	to	IN
cord-001142-puj74k7y	12	16	date	date	NN
cord-001142-puj74k7y	12	17	.	.	.
cord-001142-puj74k7y	13	1	The	the	DT
cord-001142-puj74k7y	13	2	general	general	JJ
cord-001142-puj74k7y	13	3	structural	structural	JJ
cord-001142-puj74k7y	13	4	features	feature	NNS
cord-001142-puj74k7y	13	5	and	and	CC
cord-001142-puj74k7y	13	6	genome	genome	NN
cord-001142-puj74k7y	13	7	organization	organization	NN
cord-001142-puj74k7y	13	8	of	of	IN
cord-001142-puj74k7y	13	9	influenza	influenza	NN
cord-001142-puj74k7y	13	10	viruses	virus	NNS
cord-001142-puj74k7y	13	11	suggest	suggest	VBP
cord-001142-puj74k7y	13	12	that	that	IN
cord-001142-puj74k7y	13	13	they	-PRON-	PRP
cord-001142-puj74k7y	13	14	share	share	VBP
cord-001142-puj74k7y	13	15	a	a	DT
cord-001142-puj74k7y	13	16	common	common	JJ
cord-001142-puj74k7y	13	17	ancestor	ancestor	NN
cord-001142-puj74k7y	13	18	[	[	-LRB-
cord-001142-puj74k7y	13	19	1	1	CD
cord-001142-puj74k7y	13	20	]	]	-RRB-
cord-001142-puj74k7y	13	21	.	.	.
cord-001142-puj74k7y	14	1	The	the	DT
cord-001142-puj74k7y	14	2	genome	genome	NN
cord-001142-puj74k7y	14	3	of	of	IN
cord-001142-puj74k7y	14	4	influenza	influenza	NN
cord-001142-puj74k7y	14	5	A	a	NN
cord-001142-puj74k7y	14	6	and	and	CC
cord-001142-puj74k7y	14	7	B	b	NN
cord-001142-puj74k7y	14	8	viruses	virus	NNS
cord-001142-puj74k7y	14	9	consists	consist	VBZ
cord-001142-puj74k7y	14	10	of	of	IN
cord-001142-puj74k7y	14	11	eight	eight	CD
cord-001142-puj74k7y	14	12	segments	segment	NNS
cord-001142-puj74k7y	14	13	,	,	,
cord-001142-puj74k7y	14	14	whereas	whereas	IN
cord-001142-puj74k7y	14	15	that	that	DT
cord-001142-puj74k7y	14	16	of	of	IN
cord-001142-puj74k7y	14	17	influenza	influenza	NN
cord-001142-puj74k7y	14	18	C	C	NNP
cord-001142-puj74k7y	14	19	virus	virus	NN
cord-001142-puj74k7y	14	20	has	have	VBZ
cord-001142-puj74k7y	14	21	only	only	RB
cord-001142-puj74k7y	14	22	seven	seven	CD
cord-001142-puj74k7y	14	23	segments	segment	NNS
cord-001142-puj74k7y	14	24	since	since	IN
cord-001142-puj74k7y	14	25	it	-PRON-	PRP
cord-001142-puj74k7y	14	26	has	have	VBZ
cord-001142-puj74k7y	14	27	a	a	DT
cord-001142-puj74k7y	14	28	single	single	JJ
cord-001142-puj74k7y	14	29	envelope	envelope	NN
cord-001142-puj74k7y	14	30	glycoprotein	glycoprotein	NN
cord-001142-puj74k7y	14	31	(	(	-LRB-
cord-001142-puj74k7y	14	32	HEF	HEF	NNP
cord-001142-puj74k7y	14	33	)	)	-RRB-
cord-001142-puj74k7y	14	34	instead	instead	RB
cord-001142-puj74k7y	14	35	of	of	IN
cord-001142-puj74k7y	14	36	two	two	CD
cord-001142-puj74k7y	14	37	for	for	IN
cord-001142-puj74k7y	14	38	type	type	NN
cord-001142-puj74k7y	14	39	A	a	NN
cord-001142-puj74k7y	14	40	and	and	CC
cord-001142-puj74k7y	14	41	B	b	NN
cord-001142-puj74k7y	14	42	viruses	virus	NNS
cord-001142-puj74k7y	14	43	(	(	-LRB-
cord-001142-puj74k7y	14	44	HA	HA	NNP
cord-001142-puj74k7y	14	45	and	and	CC
cord-001142-puj74k7y	14	46	NA	NA	NNP
cord-001142-puj74k7y	14	47	)	)	-RRB-
cord-001142-puj74k7y	14	48	[	[	-LRB-
cord-001142-puj74k7y	14	49	2	2	CD
cord-001142-puj74k7y	14	50	]	]	-RRB-
cord-001142-puj74k7y	14	51	.	.	.
cord-001142-puj74k7y	15	1	The	the	DT
cord-001142-puj74k7y	15	2	genomic	genomic	JJ
cord-001142-puj74k7y	15	3	viral	viral	JJ
cord-001142-puj74k7y	15	4	RNAs	rna	NNS
cord-001142-puj74k7y	15	5	(	(	-LRB-
cord-001142-puj74k7y	15	6	vRNAs	vrna	NNS
cord-001142-puj74k7y	15	7	)	)	-RRB-
cord-001142-puj74k7y	15	8	are	be	VBP
cord-001142-puj74k7y	15	9	associated	associate	VBN
cord-001142-puj74k7y	15	10	with	with	IN
cord-001142-puj74k7y	15	11	the	the	DT
cord-001142-puj74k7y	15	12	nucleoprotein	nucleoprotein	NNP
cord-001142-puj74k7y	15	13	(	(	-LRB-
cord-001142-puj74k7y	15	14	NP	NP	NNP
cord-001142-puj74k7y	15	15	)	)	-RRB-
cord-001142-puj74k7y	15	16	and	and	CC
cord-001142-puj74k7y	15	17	the	the	DT
cord-001142-puj74k7y	15	18	polymerase	polymerase	NN
cord-001142-puj74k7y	15	19	complex	complex	NN
cord-001142-puj74k7y	15	20	(	(	-LRB-
cord-001142-puj74k7y	15	21	P	p	NN
cord-001142-puj74k7y	15	22	)	)	-RRB-
cord-001142-puj74k7y	15	23	.	.	.
cord-001142-puj74k7y	16	1	The	the	DT
cord-001142-puj74k7y	16	2	latter	latter	JJ
cord-001142-puj74k7y	16	3	is	be	VBZ
cord-001142-puj74k7y	16	4	formed	form	VBN
cord-001142-puj74k7y	16	5	by	by	IN
cord-001142-puj74k7y	16	6	three	three	CD
cord-001142-puj74k7y	16	7	subunits	subunit	NNS
cord-001142-puj74k7y	16	8	named	name	VBN
cord-001142-puj74k7y	16	9	PB1	PB1	NNP
cord-001142-puj74k7y	16	10	,	,	,
cord-001142-puj74k7y	16	11	PB2	PB2	NNP
cord-001142-puj74k7y	16	12	and	and	CC
cord-001142-puj74k7y	16	13	PA	PA	NNP
cord-001142-puj74k7y	16	14	for	for	IN
cord-001142-puj74k7y	16	15	influenza	influenza	NN
cord-001142-puj74k7y	16	16	A	a	NN
cord-001142-puj74k7y	16	17	and	and	CC
cord-001142-puj74k7y	16	18	B	b	NN
cord-001142-puj74k7y	16	19	viruses	virus	NNS
cord-001142-puj74k7y	16	20	and	and	CC
cord-001142-puj74k7y	16	21	PB1	pb1	NN
cord-001142-puj74k7y	16	22	,	,	,
cord-001142-puj74k7y	16	23	PB2	PB2	NNP
cord-001142-puj74k7y	16	24	and	and	CC
cord-001142-puj74k7y	16	25	P3	P3	NNP
cord-001142-puj74k7y	16	26	for	for	IN
cord-001142-puj74k7y	16	27	influenza	influenza	NN
cord-001142-puj74k7y	16	28	C	C	NNP
cord-001142-puj74k7y	16	29	virus	virus	NN
cord-001142-puj74k7y	16	30	,	,	,
cord-001142-puj74k7y	16	31	respectively	respectively	RB
cord-001142-puj74k7y	16	32	.	.	.
cord-001142-puj74k7y	17	1	In	in	IN
cord-001142-puj74k7y	17	2	the	the	DT
cord-001142-puj74k7y	17	3	nucleus	nucleus	NN
cord-001142-puj74k7y	17	4	of	of	IN
cord-001142-puj74k7y	17	5	infected	infected	JJ
cord-001142-puj74k7y	17	6	cells	cell	NNS
cord-001142-puj74k7y	17	7	,	,	,
cord-001142-puj74k7y	17	8	viral	viral	JJ
cord-001142-puj74k7y	17	9	messenger	messenger	NN
cord-001142-puj74k7y	17	10	RNA	RNA	NNP
cord-001142-puj74k7y	17	11	(	(	-LRB-
cord-001142-puj74k7y	17	12	mRNA	mRNA	NNP
cord-001142-puj74k7y	17	13	)	)	-RRB-
cord-001142-puj74k7y	17	14	synthesis	synthesis	NN
cord-001142-puj74k7y	17	15	is	be	VBZ
cord-001142-puj74k7y	17	16	initiated	initiate	VBN
cord-001142-puj74k7y	17	17	with	with	IN
cord-001142-puj74k7y	17	18	capped	cap	VBN
cord-001142-puj74k7y	17	19	RNA	RNA	NNP
cord-001142-puj74k7y	17	20	primers	primer	NNS
cord-001142-puj74k7y	17	21	that	that	WDT
cord-001142-puj74k7y	17	22	are	be	VBP
cord-001142-puj74k7y	17	23	cleaved	cleave	VBN
cord-001142-puj74k7y	17	24	from	from	IN
cord-001142-puj74k7y	17	25	host	host	NN
cord-001142-puj74k7y	17	26	cell	cell	NN
cord-001142-puj74k7y	17	27	mRNAs	mRNAs	.
cord-001142-puj74k7y	18	1	(	(	-LRB-
cord-001142-puj74k7y	18	2	capsnatching	capsnatching	NN
cord-001142-puj74k7y	18	3	mechanism	mechanism	NN
cord-001142-puj74k7y	18	4	)	)	-RRB-
cord-001142-puj74k7y	18	5	,	,	,
cord-001142-puj74k7y	18	6	and	and	CC
cord-001142-puj74k7y	18	7	terminates	terminate	VBZ
cord-001142-puj74k7y	18	8	17	17	CD
cord-001142-puj74k7y	18	9	to	to	TO
cord-001142-puj74k7y	18	10	22	22	CD
cord-001142-puj74k7y	18	11	nucleotides	nucleotide	NNS
cord-001142-puj74k7y	18	12	(	(	-LRB-
cord-001142-puj74k7y	18	13	nt	nt	RB
cord-001142-puj74k7y	18	14	)	)	-RRB-
cord-001142-puj74k7y	18	15	upstream	upstream	RB
cord-001142-puj74k7y	18	16	of	of	IN
cord-001142-puj74k7y	18	17	the	the	DT
cord-001142-puj74k7y	18	18	genomic	genomic	JJ
cord-001142-puj74k7y	18	19	vRNA	vRNA	HYPH
cord-001142-puj74k7y	18	20	template	template	NN
cord-001142-puj74k7y	18	21	5	5	CD
cord-001142-puj74k7y	18	22	'	'	''
cord-001142-puj74k7y	19	1	end	end	VB
cord-001142-puj74k7y	19	2	at	at	IN
cord-001142-puj74k7y	19	3	a	a	DT
cord-001142-puj74k7y	19	4	stretch	stretch	NN
cord-001142-puj74k7y	19	5	of	of	IN
cord-001142-puj74k7y	19	6	five	five	CD
cord-001142-puj74k7y	19	7	to	to	TO
cord-001142-puj74k7y	19	8	seven	seven	CD
cord-001142-puj74k7y	19	9	uridine	uridine	NN
cord-001142-puj74k7y	19	10	residues	residue	NNS
cord-001142-puj74k7y	19	11	used	use	VBN
cord-001142-puj74k7y	19	12	as	as	IN
cord-001142-puj74k7y	19	13	a	a	DT
cord-001142-puj74k7y	19	14	polyadenylation	polyadenylation	NN
cord-001142-puj74k7y	19	15	signal	signal	NN
cord-001142-puj74k7y	19	16	.	.	.
cord-001142-puj74k7y	20	1	Genomic	genomic	JJ
cord-001142-puj74k7y	20	2	vRNA	vrna	IN
cord-001142-puj74k7y	20	3	replication	replication	NN
cord-001142-puj74k7y	20	4	requires	require	VBZ
cord-001142-puj74k7y	20	5	a	a	DT
cord-001142-puj74k7y	20	6	full	full	JJ
cord-001142-puj74k7y	20	7	-	-	HYPH
cord-001142-puj74k7y	20	8	length	length	NN
cord-001142-puj74k7y	20	9	positive	positive	JJ
cord-001142-puj74k7y	20	10	-	-	HYPH
cord-001142-puj74k7y	20	11	sense	sense	NN
cord-001142-puj74k7y	20	12	RNA	rna	NN
cord-001142-puj74k7y	20	13	template	template	NN
cord-001142-puj74k7y	20	14	(	(	-LRB-
cord-001142-puj74k7y	20	15	complementary	complementary	JJ
cord-001142-puj74k7y	20	16	RNA	RNA	NNP
cord-001142-puj74k7y	20	17	or	or	CC
cord-001142-puj74k7y	20	18	cRNA	cRNA	NNP
cord-001142-puj74k7y	20	19	)	)	-RRB-
cord-001142-puj74k7y	20	20	,	,	,
cord-001142-puj74k7y	20	21	and	and	CC
cord-001142-puj74k7y	20	22	both	both	DT
cord-001142-puj74k7y	20	23	cRNA	cRNA	NNS
cord-001142-puj74k7y	20	24	and	and	CC
cord-001142-puj74k7y	20	25	vRNA	vrna	JJ
cord-001142-puj74k7y	20	26	syntheses	synthesis	NNS
cord-001142-puj74k7y	20	27	are	be	VBP
cord-001142-puj74k7y	20	28	primer	primer	NN
cord-001142-puj74k7y	20	29	-	-	HYPH
cord-001142-puj74k7y	20	30	independent	independent	JJ
cord-001142-puj74k7y	20	31	[	[	-LRB-
cord-001142-puj74k7y	20	32	2	2	CD
cord-001142-puj74k7y	20	33	]	]	-RRB-
cord-001142-puj74k7y	20	34	.	.	.
cord-001142-puj74k7y	21	1	The	the	DT
cord-001142-puj74k7y	21	2	coding	code	VBG
cord-001142-puj74k7y	21	3	region	region	NN
cord-001142-puj74k7y	21	4	of	of	IN
cord-001142-puj74k7y	21	5	each	each	DT
cord-001142-puj74k7y	21	6	genomic	genomic	JJ
cord-001142-puj74k7y	21	7	vRNA	vrna	NN
cord-001142-puj74k7y	21	8	is	be	VBZ
cord-001142-puj74k7y	21	9	flanked	flank	VBN
cord-001142-puj74k7y	21	10	by	by	IN
cord-001142-puj74k7y	21	11	noncoding	noncode	VBG
cord-001142-puj74k7y	21	12	(	(	-LRB-
cord-001142-puj74k7y	21	13	NC	NC	NNP
cord-001142-puj74k7y	21	14	)	)	-RRB-
cord-001142-puj74k7y	21	15	sequences	sequence	NNS
cord-001142-puj74k7y	21	16	that	that	WDT
cord-001142-puj74k7y	21	17	are	be	VBP
cord-001142-puj74k7y	21	18	divided	divide	VBN
cord-001142-puj74k7y	21	19	into	into	IN
cord-001142-puj74k7y	21	20	conserved	conserved	JJ
cord-001142-puj74k7y	21	21	and	and	CC
cord-001142-puj74k7y	21	22	non	non	JJ
cord-001142-puj74k7y	21	23	conserved	conserved	JJ
cord-001142-puj74k7y	21	24	parts	part	NNS
cord-001142-puj74k7y	21	25	[	[	-LRB-
cord-001142-puj74k7y	21	26	3	3	CD
cord-001142-puj74k7y	21	27	]	]	-RRB-
cord-001142-puj74k7y	21	28	.	.	.
cord-001142-puj74k7y	22	1	The	the	DT
cord-001142-puj74k7y	22	2	length	length	NN
cord-001142-puj74k7y	22	3	of	of	IN
cord-001142-puj74k7y	22	4	the	the	DT
cord-001142-puj74k7y	22	5	NC	NC	NNP
cord-001142-puj74k7y	22	6	sequences	sequence	NNS
cord-001142-puj74k7y	22	7	differs	differ	VBZ
cord-001142-puj74k7y	22	8	for	for	IN
cord-001142-puj74k7y	22	9	each	each	DT
cord-001142-puj74k7y	22	10	segment	segment	NN
cord-001142-puj74k7y	22	11	and	and	CC
cord-001142-puj74k7y	22	12	also	also	RB
cord-001142-puj74k7y	22	13	varies	vary	VBZ
cord-001142-puj74k7y	22	14	between	between	IN
cord-001142-puj74k7y	22	15	virus	virus	NN
cord-001142-puj74k7y	22	16	types	type	NNS
cord-001142-puj74k7y	22	17	[	[	-LRB-
cord-001142-puj74k7y	22	18	4	4	CD
cord-001142-puj74k7y	22	19	,	,	,
cord-001142-puj74k7y	22	20	5	5	CD
cord-001142-puj74k7y	22	21	]	]	-RRB-
cord-001142-puj74k7y	22	22	.	.	.
cord-001142-puj74k7y	23	1	For	for	IN
cord-001142-puj74k7y	23	2	the	the	DT
cord-001142-puj74k7y	23	3	3	3	CD
cord-001142-puj74k7y	23	4	'	'	''
cord-001142-puj74k7y	23	5	and	and	CC
cord-001142-puj74k7y	23	6	5	5	CD
cord-001142-puj74k7y	23	7	'	'	POS
cord-001142-puj74k7y	23	8	ends	end	NNS
cord-001142-puj74k7y	23	9	,	,	,
cord-001142-puj74k7y	23	10	respectively	respectively	RB
cord-001142-puj74k7y	23	11	,	,	,
cord-001142-puj74k7y	23	12	the	the	DT
cord-001142-puj74k7y	23	13	conserved	conserved	JJ
cord-001142-puj74k7y	23	14	parts	part	NNS
cord-001142-puj74k7y	23	15	are	be	VBP
cord-001142-puj74k7y	23	16	12	12	CD
cord-001142-puj74k7y	23	17	and	and	CC
cord-001142-puj74k7y	23	18	13	13	CD
cord-001142-puj74k7y	23	19	nt	nt	NN
cord-001142-puj74k7y	23	20	for	for	IN
cord-001142-puj74k7y	23	21	type	type	NN
cord-001142-puj74k7y	23	22	A	a	NN
cord-001142-puj74k7y	23	23	,	,	,
cord-001142-puj74k7y	23	24	12	12	CD
cord-001142-puj74k7y	23	25	and	and	CC
cord-001142-puj74k7y	23	26	11nt	11nt	JJ
cord-001142-puj74k7y	23	27	for	for	IN
cord-001142-puj74k7y	23	28	type	type	NN
cord-001142-puj74k7y	23	29	B	b	NN
cord-001142-puj74k7y	23	30	and	and	CC
cord-001142-puj74k7y	23	31	11	11	CD
cord-001142-puj74k7y	23	32	and	and	CC
cord-001142-puj74k7y	23	33	12	12	CD
cord-001142-puj74k7y	23	34	nt	nt	NN
cord-001142-puj74k7y	23	35	for	for	IN
cord-001142-puj74k7y	23	36	type	type	NN
cord-001142-puj74k7y	23	37	C	C	NNP
cord-001142-puj74k7y	23	38	influenza	influenza	NN
cord-001142-puj74k7y	23	39	viruses	virus	NNS
cord-001142-puj74k7y	23	40	[	[	-LRB-
cord-001142-puj74k7y	23	41	6	6	CD
cord-001142-puj74k7y	23	42	]	]	-RRB-
cord-001142-puj74k7y	23	43	[	[	-LRB-
cord-001142-puj74k7y	23	44	7	7	CD
cord-001142-puj74k7y	23	45	]	]	-RRB-
cord-001142-puj74k7y	23	46	[	[	-LRB-
cord-001142-puj74k7y	23	47	8	8	CD
cord-001142-puj74k7y	23	48	]	]	-RRB-
cord-001142-puj74k7y	23	49	.	.	.
cord-001142-puj74k7y	24	1	The	the	DT
cord-001142-puj74k7y	24	2	5	5	CD
cord-001142-puj74k7y	24	3	'	'	''
cord-001142-puj74k7y	24	4	and	and	CC
cord-001142-puj74k7y	24	5	3	3	CD
cord-001142-puj74k7y	24	6	'	'	''
cord-001142-puj74k7y	24	7	NC	NC	NNP
cord-001142-puj74k7y	24	8	sequences	sequence	NNS
cord-001142-puj74k7y	24	9	base	base	NN
cord-001142-puj74k7y	24	10	-	-	HYPH
cord-001142-puj74k7y	24	11	pair	pair	NN
cord-001142-puj74k7y	24	12	to	to	TO
cord-001142-puj74k7y	24	13	form	form	VB
cord-001142-puj74k7y	24	14	two	two	CD
cord-001142-puj74k7y	24	15	elements	element	NNS
cord-001142-puj74k7y	24	16	:	:	:
cord-001142-puj74k7y	24	17	the	the	DT
cord-001142-puj74k7y	24	18	proximal	proximal	JJ
cord-001142-puj74k7y	24	19	element	element	NN
cord-001142-puj74k7y	24	20	or	or	CC
cord-001142-puj74k7y	24	21	region	region	NN
cord-001142-puj74k7y	24	22	I	-PRON-	PRP
cord-001142-puj74k7y	24	23	(	(	-LRB-
cord-001142-puj74k7y	24	24	nt	nt	RB
cord-001142-puj74k7y	24	25	1	1	CD
cord-001142-puj74k7y	24	26	-	-	SYM
cord-001142-puj74k7y	24	27	9	9	CD
cord-001142-puj74k7y	24	28	of	of	IN
cord-001142-puj74k7y	24	29	the	the	DT
cord-001142-puj74k7y	24	30	3	3	CD
cord-001142-puj74k7y	24	31	'	'	''
cord-001142-puj74k7y	24	32	and	and	CC
cord-001142-puj74k7y	24	33	5	5	CD
cord-001142-puj74k7y	24	34	'	'	POS
cord-001142-puj74k7y	24	35	ends	end	NNS
cord-001142-puj74k7y	24	36	)	)	-RRB-
cord-001142-puj74k7y	24	37	and	and	CC
cord-001142-puj74k7y	24	38	the	the	DT
cord-001142-puj74k7y	24	39	distal	distal	JJ
cord-001142-puj74k7y	24	40	element	element	NN
cord-001142-puj74k7y	24	41	or	or	CC
cord-001142-puj74k7y	24	42	region	region	NN
cord-001142-puj74k7y	24	43	II	ii	CD
cord-001142-puj74k7y	24	44	involving	involve	VBG
cord-001142-puj74k7y	24	45	sequences	sequence	NNS
cord-001142-puj74k7y	24	46	downstream	downstream	RB
cord-001142-puj74k7y	24	47	of	of	IN
cord-001142-puj74k7y	24	48	nt	nt	RB
cord-001142-puj74k7y	24	49	10	10	CD
cord-001142-puj74k7y	24	50	and	and	CC
cord-001142-puj74k7y	24	51	11	11	CD
cord-001142-puj74k7y	24	52	from	from	IN
cord-001142-puj74k7y	24	53	the	the	DT
cord-001142-puj74k7y	24	54	3	3	CD
cord-001142-puj74k7y	24	55	'	'	''
cord-001142-puj74k7y	24	56	and	and	CC
cord-001142-puj74k7y	24	57	5	5	CD
cord-001142-puj74k7y	24	58	'	'	POS
cord-001142-puj74k7y	24	59	ends	end	NNS
cord-001142-puj74k7y	24	60	,	,	,
cord-001142-puj74k7y	24	61	respectively	respectively	RB
cord-001142-puj74k7y	24	62	[	[	-LRB-
cord-001142-puj74k7y	24	63	9	9	CD
cord-001142-puj74k7y	24	64	]	]	-RRB-
cord-001142-puj74k7y	24	65	.	.	.
cord-001142-puj74k7y	25	1	Two	two	CD
cord-001142-puj74k7y	25	2	main	main	JJ
cord-001142-puj74k7y	25	3	secondary	secondary	JJ
cord-001142-puj74k7y	25	4	structures	structure	NNS
cord-001142-puj74k7y	25	5	have	have	VBP
cord-001142-puj74k7y	25	6	been	be	VBN
cord-001142-puj74k7y	25	7	described	describe	VBN
cord-001142-puj74k7y	25	8	:	:	:
cord-001142-puj74k7y	25	9	the	the	DT
cord-001142-puj74k7y	25	10	"	"	``
cord-001142-puj74k7y	25	11	panhandle	panhandle	NNP
cord-001142-puj74k7y	25	12	structure	structure	NN
cord-001142-puj74k7y	25	13	"	"	''
cord-001142-puj74k7y	25	14	resulting	result	VBG
cord-001142-puj74k7y	25	15	from	from	IN
cord-001142-puj74k7y	25	16	complete	complete	JJ
cord-001142-puj74k7y	25	17	base	base	NN
cord-001142-puj74k7y	25	18	-	-	HYPH
cord-001142-puj74k7y	25	19	pairing	pairing	NN
cord-001142-puj74k7y	25	20	between	between	IN
cord-001142-puj74k7y	25	21	the	the	DT
cord-001142-puj74k7y	25	22	two	two	CD
cord-001142-puj74k7y	25	23	ends	end	NNS
cord-001142-puj74k7y	25	24	[	[	-LRB-
cord-001142-puj74k7y	25	25	10	10	CD
cord-001142-puj74k7y	25	26	]	]	-RRB-
cord-001142-puj74k7y	25	27	[	[	-LRB-
cord-001142-puj74k7y	25	28	11	11	CD
cord-001142-puj74k7y	25	29	]	]	-RRB-
cord-001142-puj74k7y	25	30	[	[	-LRB-
cord-001142-puj74k7y	25	31	12	12	CD
cord-001142-puj74k7y	25	32	]	]	-RRB-
cord-001142-puj74k7y	25	33	[	[	-LRB-
cord-001142-puj74k7y	25	34	13	13	CD
cord-001142-puj74k7y	25	35	]	]	-RRB-
cord-001142-puj74k7y	25	36	[	[	-LRB-
cord-001142-puj74k7y	25	37	14	14	CD
cord-001142-puj74k7y	25	38	]	]	-RRB-
cord-001142-puj74k7y	25	39	and	and	CC
cord-001142-puj74k7y	25	40	the	the	DT
cord-001142-puj74k7y	25	41	"	"	``
cord-001142-puj74k7y	25	42	corkscrew	corkscrew	JJ
cord-001142-puj74k7y	25	43	structure	structure	NN
cord-001142-puj74k7y	25	44	"	"	''
cord-001142-puj74k7y	25	45	,	,	,
cord-001142-puj74k7y	25	46	where	where	WRB
cord-001142-puj74k7y	25	47	the	the	DT
cord-001142-puj74k7y	25	48	proximal	proximal	JJ
cord-001142-puj74k7y	25	49	element	element	NN
cord-001142-puj74k7y	25	50	form	form	NN
cord-001142-puj74k7y	25	51	hairpin	hairpin	NN
cord-001142-puj74k7y	25	52	loops	loop	NNS
cord-001142-puj74k7y	25	53	[	[	-LRB-
cord-001142-puj74k7y	25	54	15	15	CD
cord-001142-puj74k7y	25	55	]	]	-RRB-
cord-001142-puj74k7y	25	56	[	[	-LRB-
cord-001142-puj74k7y	25	57	16	16	CD
cord-001142-puj74k7y	25	58	]	]	-RRB-
cord-001142-puj74k7y	25	59	[	[	-LRB-
cord-001142-puj74k7y	25	60	17	17	CD
cord-001142-puj74k7y	25	61	]	]	-RRB-
cord-001142-puj74k7y	25	62	[	[	-LRB-
cord-001142-puj74k7y	25	63	18	18	CD
cord-001142-puj74k7y	25	64	]	]	-RRB-
cord-001142-puj74k7y	25	65	.	.	.
cord-001142-puj74k7y	26	1	These	these	DT
cord-001142-puj74k7y	26	2	conformations	conformation	NNS
cord-001142-puj74k7y	26	3	are	be	VBP
cord-001142-puj74k7y	26	4	known	know	VBN
cord-001142-puj74k7y	26	5	to	to	TO
cord-001142-puj74k7y	26	6	be	be	VB
cord-001142-puj74k7y	26	7	critical	critical	JJ
cord-001142-puj74k7y	26	8	for	for	IN
cord-001142-puj74k7y	26	9	transcription	transcription	NN
cord-001142-puj74k7y	26	10	and	and	CC
cord-001142-puj74k7y	26	11	replication	replication	NN
cord-001142-puj74k7y	26	12	of	of	IN
cord-001142-puj74k7y	26	13	the	the	DT
cord-001142-puj74k7y	26	14	vRNAs	vrna	NNS
cord-001142-puj74k7y	26	15	[	[	-LRB-
cord-001142-puj74k7y	26	16	9	9	CD
cord-001142-puj74k7y	26	17	]	]	-RRB-
cord-001142-puj74k7y	26	18	.	.	.
cord-001142-puj74k7y	27	1	To	to	TO
cord-001142-puj74k7y	27	2	investigate	investigate	VB
cord-001142-puj74k7y	27	3	whether	whether	IN
cord-001142-puj74k7y	27	4	,	,	,
cord-001142-puj74k7y	27	5	or	or	CC
cord-001142-puj74k7y	27	6	not	not	RB
cord-001142-puj74k7y	27	7	,	,	,
cord-001142-puj74k7y	27	8	and	and	CC
cord-001142-puj74k7y	27	9	how	how	WRB
cord-001142-puj74k7y	27	10	the	the	DT
cord-001142-puj74k7y	27	11	complete	complete	JJ
cord-001142-puj74k7y	27	12	NC	NC	NNP
cord-001142-puj74k7y	27	13	regions	region	NNS
cord-001142-puj74k7y	27	14	of	of	IN
cord-001142-puj74k7y	27	15	a	a	DT
cord-001142-puj74k7y	27	16	given	give	VBN
cord-001142-puj74k7y	27	17	segment	segment	NN
cord-001142-puj74k7y	27	18	are	be	VBP
cord-001142-puj74k7y	27	19	involved	involve	VBN
cord-001142-puj74k7y	27	20	in	in	IN
cord-001142-puj74k7y	27	21	type	type	NN
cord-001142-puj74k7y	27	22	specificity	specificity	NN
cord-001142-puj74k7y	27	23	,	,	,
cord-001142-puj74k7y	27	24	we	-PRON-	PRP
cord-001142-puj74k7y	27	25	attempted	attempt	VBD
cord-001142-puj74k7y	27	26	to	to	TO
cord-001142-puj74k7y	27	27	rescue	rescue	VB
cord-001142-puj74k7y	27	28	,	,	,
cord-001142-puj74k7y	27	29	by	by	IN
cord-001142-puj74k7y	27	30	reverse	reverse	JJ
cord-001142-puj74k7y	27	31	genetics	genetic	NNS
cord-001142-puj74k7y	27	32	,	,	,
cord-001142-puj74k7y	27	33	type	type	NN
cord-001142-puj74k7y	27	34	A	a	NN
cord-001142-puj74k7y	27	35	and	and	CC
cord-001142-puj74k7y	27	36	C	c	NN
cord-001142-puj74k7y	27	37	influenza	influenza	NN
cord-001142-puj74k7y	27	38	viruses	virus	NNS
cord-001142-puj74k7y	27	39	with	with	IN
cord-001142-puj74k7y	27	40	chimeric	chimeric	JJ
cord-001142-puj74k7y	27	41	non	non	JJ
cord-001142-puj74k7y	27	42	-	-	JJ
cord-001142-puj74k7y	27	43	coding	coding	JJ
cord-001142-puj74k7y	27	44	sequences	sequence	NNS
cord-001142-puj74k7y	27	45	.	.	.
cord-001142-puj74k7y	28	1	All	all	DT
cord-001142-puj74k7y	28	2	experiments	experiment	NNS
cord-001142-puj74k7y	28	3	were	be	VBD
cord-001142-puj74k7y	28	4	based	base	VBN
cord-001142-puj74k7y	28	5	on	on	IN
cord-001142-puj74k7y	28	6	the	the	DT
cord-001142-puj74k7y	28	7	NS	NS	NNP
cord-001142-puj74k7y	28	8	segment	segment	NN
cord-001142-puj74k7y	28	9	,	,	,
cord-001142-puj74k7y	28	10	the	the	DT
cord-001142-puj74k7y	28	11	smallest	small	JJS
cord-001142-puj74k7y	28	12	segment	segment	NN
cord-001142-puj74k7y	28	13	for	for	IN
cord-001142-puj74k7y	28	14	both	both	DT
cord-001142-puj74k7y	28	15	influenza	influenza	NN
cord-001142-puj74k7y	28	16	virus	virus	NN
cord-001142-puj74k7y	28	17	types	type	NNS
cord-001142-puj74k7y	28	18	and	and	CC
cord-001142-puj74k7y	28	19	for	for	IN
cord-001142-puj74k7y	28	20	which	which	WDT
cord-001142-puj74k7y	28	21	the	the	DT
cord-001142-puj74k7y	28	22	NC	NC	NNP
cord-001142-puj74k7y	28	23	regions	region	NNS
cord-001142-puj74k7y	28	24	of	of	IN
cord-001142-puj74k7y	28	25	both	both	DT
cord-001142-puj74k7y	28	26	viruses	virus	NNS
cord-001142-puj74k7y	28	27	are	be	VBP
cord-001142-puj74k7y	28	28	quite	quite	RB
cord-001142-puj74k7y	28	29	similar	similar	JJ
cord-001142-puj74k7y	28	30	in	in	IN
cord-001142-puj74k7y	28	31	length	length	NN
cord-001142-puj74k7y	28	32	.	.	.
cord-001142-puj74k7y	29	1	We	-PRON-	PRP
cord-001142-puj74k7y	29	2	showed	show	VBD
cord-001142-puj74k7y	29	3	that	that	DT
cord-001142-puj74k7y	29	4	type	type	NN
cord-001142-puj74k7y	29	5	specificity	specificity	NN
cord-001142-puj74k7y	29	6	of	of	IN
cord-001142-puj74k7y	29	7	the	the	DT
cord-001142-puj74k7y	29	8	proximal	proximal	JJ
cord-001142-puj74k7y	29	9	element	element	NN
cord-001142-puj74k7y	29	10	is	be	VBZ
cord-001142-puj74k7y	29	11	critical	critical	JJ
cord-001142-puj74k7y	29	12	to	to	TO
cord-001142-puj74k7y	29	13	rescue	rescue	VB
cord-001142-puj74k7y	29	14	infectious	infectious	JJ
cord-001142-puj74k7y	29	15	viruses	virus	NNS
cord-001142-puj74k7y	29	16	,	,	,
cord-001142-puj74k7y	29	17	and	and	CC
cord-001142-puj74k7y	29	18	that	that	IN
cord-001142-puj74k7y	29	19	the	the	DT
cord-001142-puj74k7y	29	20	distal	distal	JJ
cord-001142-puj74k7y	29	21	element	element	NN
cord-001142-puj74k7y	29	22	might	may	MD
cord-001142-puj74k7y	29	23	modulate	modulate	VB
cord-001142-puj74k7y	29	24	viral	viral	JJ
cord-001142-puj74k7y	29	25	transcription	transcription	NN
cord-001142-puj74k7y	29	26	.	.	.
cord-001142-puj74k7y	30	1	The	the	DT
cord-001142-puj74k7y	30	2	12-or	12-or	CD
cord-001142-puj74k7y	30	3	11-plasmids	11-plasmids	CD
cord-001142-puj74k7y	30	4	based	base	VBN
cord-001142-puj74k7y	30	5	reverse	reverse	JJ
cord-001142-puj74k7y	30	6	genetic	genetic	JJ
cord-001142-puj74k7y	30	7	systems	system	NNS
cord-001142-puj74k7y	30	8	were	be	VBD
cord-001142-puj74k7y	30	9	used	use	VBN
cord-001142-puj74k7y	30	10	to	to	TO
cord-001142-puj74k7y	30	11	produce	produce	VB
cord-001142-puj74k7y	30	12	recombinant	recombinant	JJ
cord-001142-puj74k7y	30	13	type	type	NN
cord-001142-puj74k7y	30	14	A	a	NN
cord-001142-puj74k7y	30	15	(	(	-LRB-
cord-001142-puj74k7y	30	16	A	a	NN
cord-001142-puj74k7y	30	17	/	/	SYM
cord-001142-puj74k7y	30	18	WSN/33	wsn/33	NN
cord-001142-puj74k7y	30	19	)	)	-RRB-
cord-001142-puj74k7y	30	20	and	and	CC
cord-001142-puj74k7y	30	21	type	type	NN
cord-001142-puj74k7y	30	22	C	c	NN
cord-001142-puj74k7y	30	23	(	(	-LRB-
cord-001142-puj74k7y	30	24	C	C	NNP
cord-001142-puj74k7y	30	25	/	/	SYM
cord-001142-puj74k7y	30	26	JHB/1/66	JHB/1/66	NNP
cord-001142-puj74k7y	30	27	)	)	-RRB-
cord-001142-puj74k7y	30	28	influenza	influenza	NN
cord-001142-puj74k7y	30	29	viruses	virus	NNS
cord-001142-puj74k7y	30	30	,	,	,
cord-001142-puj74k7y	30	31	and	and	CC
cord-001142-puj74k7y	30	32	were	be	VBD
cord-001142-puj74k7y	30	33	adapted	adapt	VBN
cord-001142-puj74k7y	30	34	from	from	IN
cord-001142-puj74k7y	30	35	previously	previously	RB
cord-001142-puj74k7y	30	36	described	describe	VBN
cord-001142-puj74k7y	30	37	procedures	procedure	NNS
cord-001142-puj74k7y	30	38	[	[	-LRB-
cord-001142-puj74k7y	30	39	19	19	CD
cord-001142-puj74k7y	30	40	]	]	-RRB-
cord-001142-puj74k7y	30	41	[	[	-LRB-
cord-001142-puj74k7y	30	42	20	20	CD
cord-001142-puj74k7y	30	43	]	]	-RRB-
cord-001142-puj74k7y	30	44	[	[	-LRB-
cord-001142-puj74k7y	30	45	21	21	CD
cord-001142-puj74k7y	30	46	]	]	-RRB-
cord-001142-puj74k7y	30	47	.	.	.
cord-001142-puj74k7y	31	1	The	the	DT
cord-001142-puj74k7y	31	2	modified	modified	JJ
cord-001142-puj74k7y	31	3	NS	NS	NNP
cord-001142-puj74k7y	31	4	plasmids	plasmid	NNS
cord-001142-puj74k7y	31	5	(	(	-LRB-
cord-001142-puj74k7y	31	6	chimeric	chimeric	JJ
cord-001142-puj74k7y	31	7	)	)	-RRB-
cord-001142-puj74k7y	31	8	were	be	VBD
cord-001142-puj74k7y	31	9	constructed	construct	VBN
cord-001142-puj74k7y	31	10	by	by	IN
cord-001142-puj74k7y	31	11	PCR	PCR	NNP
cord-001142-puj74k7y	31	12	.	.	.
cord-001142-puj74k7y	32	1	Point	point	NN
cord-001142-puj74k7y	32	2	mutations	mutation	NNS
cord-001142-puj74k7y	32	3	in	in	IN
cord-001142-puj74k7y	32	4	the	the	DT
cord-001142-puj74k7y	32	5	NS	NS	NNP
cord-001142-puj74k7y	32	6	NC	NC	NNP
cord-001142-puj74k7y	32	7	regions	region	NNS
cord-001142-puj74k7y	32	8	were	be	VBD
cord-001142-puj74k7y	32	9	introduced	introduce	VBN
cord-001142-puj74k7y	32	10	by	by	IN
cord-001142-puj74k7y	32	11	directed	direct	VBN
cord-001142-puj74k7y	32	12	mutagenesis	mutagenesis	NN
cord-001142-puj74k7y	32	13	using	use	VBG
cord-001142-puj74k7y	32	14	Quikchange	Quikchange	NNP
cord-001142-puj74k7y	32	15	II	II	NNP
cord-001142-puj74k7y	32	16	site	site	NN
cord-001142-puj74k7y	32	17	-	-	HYPH
cord-001142-puj74k7y	32	18	Directed	direct	VBN
cord-001142-puj74k7y	32	19	mutagenesis	mutagenesis	NN
cord-001142-puj74k7y	32	20	kit	kit	NN
cord-001142-puj74k7y	32	21	(	(	-LRB-
cord-001142-puj74k7y	32	22	Agilent	Agilent	NNP
cord-001142-puj74k7y	32	23	Technologies	Technologies	NNPS
cord-001142-puj74k7y	32	24	)	)	-RRB-
cord-001142-puj74k7y	32	25	according	accord	VBG
cord-001142-puj74k7y	32	26	to	to	IN
cord-001142-puj74k7y	32	27	the	the	DT
cord-001142-puj74k7y	32	28	manufacturer	manufacturer	NN
cord-001142-puj74k7y	32	29	's	's	POS
cord-001142-puj74k7y	32	30	instructions	instruction	NNS
cord-001142-puj74k7y	32	31	.	.	.
cord-001142-puj74k7y	33	1	The	the	DT
cord-001142-puj74k7y	33	2	primer	primer	NN
cord-001142-puj74k7y	33	3	sequences	sequence	NNS
cord-001142-puj74k7y	33	4	will	will	MD
cord-001142-puj74k7y	33	5	be	be	VB
cord-001142-puj74k7y	33	6	provided	provide	VBN
cord-001142-puj74k7y	33	7	upon	upon	IN
cord-001142-puj74k7y	33	8	request	request	NN
cord-001142-puj74k7y	33	9	.	.	.
cord-001142-puj74k7y	34	1	All	all	DT
cord-001142-puj74k7y	34	2	plasmids	plasmid	NNS
cord-001142-puj74k7y	34	3	were	be	VBD
cord-001142-puj74k7y	34	4	sequenced	sequence	VBN
cord-001142-puj74k7y	34	5	using	use	VBG
cord-001142-puj74k7y	34	6	a	a	DT
cord-001142-puj74k7y	34	7	Big	big	JJ
cord-001142-puj74k7y	34	8	Dye	dye	NN
cord-001142-puj74k7y	34	9	terminator	terminator	NN
cord-001142-puj74k7y	34	10	sequencing	sequencing	NN
cord-001142-puj74k7y	34	11	kit	kit	NN
cord-001142-puj74k7y	34	12	and	and	CC
cord-001142-puj74k7y	34	13	an	an	DT
cord-001142-puj74k7y	34	14	automated	automated	JJ
cord-001142-puj74k7y	34	15	sequencer	sequencer	NN
cord-001142-puj74k7y	34	16	(	(	-LRB-
cord-001142-puj74k7y	34	17	Perkin	Perkin	NNP
cord-001142-puj74k7y	34	18	-	-	HYPH
cord-001142-puj74k7y	34	19	Elmer	Elmer	NNP
cord-001142-puj74k7y	34	20	)	)	-RRB-
cord-001142-puj74k7y	34	21	.	.	.
cord-001142-puj74k7y	35	1	Human	human	JJ
cord-001142-puj74k7y	35	2	skin	skin	NN
cord-001142-puj74k7y	35	3	melanoma	melanoma	NN
cord-001142-puj74k7y	35	4	cells	cell	NNS
cord-001142-puj74k7y	35	5	(	(	-LRB-
cord-001142-puj74k7y	35	6	SK93/2	SK93/2	NNP
cord-001142-puj74k7y	35	7	)	)	-RRB-
cord-001142-puj74k7y	35	8	[	[	-LRB-
cord-001142-puj74k7y	35	9	22	22	CD
cord-001142-puj74k7y	35	10	]	]	-RRB-
cord-001142-puj74k7y	35	11	,	,	,
cord-001142-puj74k7y	35	12	293	293	CD
cord-001142-puj74k7y	35	13	T	T	NNP
cord-001142-puj74k7y	35	14	human	human	JJ
cord-001142-puj74k7y	35	15	embryonic	embryonic	JJ
cord-001142-puj74k7y	35	16	kidney	kidney	NN
cord-001142-puj74k7y	35	17	cells	cell	NNS
cord-001142-puj74k7y	35	18	and	and	CC
cord-001142-puj74k7y	35	19	Madin	Madin	NNP
cord-001142-puj74k7y	35	20	-	-	HYPH
cord-001142-puj74k7y	35	21	Darby	Darby	NNP
cord-001142-puj74k7y	35	22	canine	canine	JJ
cord-001142-puj74k7y	35	23	kidney	kidney	NN
cord-001142-puj74k7y	35	24	cells	cell	NNS
cord-001142-puj74k7y	36	1	(	(	-LRB-
cord-001142-puj74k7y	36	2	MDCK	MDCK	NNP
cord-001142-puj74k7y	36	3	)	)	-RRB-
cord-001142-puj74k7y	36	4	cells	cell	NNS
cord-001142-puj74k7y	36	5	were	be	VBD
cord-001142-puj74k7y	36	6	cultured	culture	VBN
cord-001142-puj74k7y	36	7	in	in	IN
cord-001142-puj74k7y	36	8	DMEM	DMEM	NNP
cord-001142-puj74k7y	36	9	supplemented	supplement	VBD
cord-001142-puj74k7y	36	10	with	with	IN
cord-001142-puj74k7y	36	11	10	10	CD
cord-001142-puj74k7y	36	12	%	%	NN
cord-001142-puj74k7y	36	13	and	and	CC
cord-001142-puj74k7y	36	14	5	5	CD
cord-001142-puj74k7y	36	15	%	%	NN
cord-001142-puj74k7y	36	16	fetal	fetal	JJ
cord-001142-puj74k7y	36	17	calf	calf	NN
cord-001142-puj74k7y	36	18	serum	serum	NN
cord-001142-puj74k7y	36	19	(	(	-LRB-
cord-001142-puj74k7y	36	20	FCS	FCS	NNP
cord-001142-puj74k7y	36	21	)	)	-RRB-
cord-001142-puj74k7y	36	22	,	,	,
cord-001142-puj74k7y	36	23	respectively	respectively	RB
cord-001142-puj74k7y	36	24	.	.	.
cord-001142-puj74k7y	37	1	All	all	DT
cord-001142-puj74k7y	37	2	cells	cell	NNS
cord-001142-puj74k7y	37	3	were	be	VBD
cord-001142-puj74k7y	37	4	grown	grow	VBN
cord-001142-puj74k7y	37	5	at	at	IN
cord-001142-puj74k7y	37	6	37	37	CD
cord-001142-puj74k7y	37	7	°	°	NN
cord-001142-puj74k7y	37	8	C	c	NN
cord-001142-puj74k7y	37	9	with	with	IN
cord-001142-puj74k7y	37	10	5	5	CD
cord-001142-puj74k7y	37	11	%	%	NN
cord-001142-puj74k7y	37	12	CO	co	NN
cord-001142-puj74k7y	37	13	2	2	CD
cord-001142-puj74k7y	37	14	.	.	.
cord-001142-puj74k7y	38	1	Plaque	plaque	NN
cord-001142-puj74k7y	38	2	assay	assay	NN
cord-001142-puj74k7y	38	3	using	use	VBG
cord-001142-puj74k7y	38	4	an	an	DT
cord-001142-puj74k7y	38	5	agarose	agarose	JJ
cord-001142-puj74k7y	38	6	overlay	overlay	NN
cord-001142-puj74k7y	38	7	was	be	VBD
cord-001142-puj74k7y	38	8	performed	perform	VBN
cord-001142-puj74k7y	38	9	for	for	IN
cord-001142-puj74k7y	38	10	titration	titration	NN
cord-001142-puj74k7y	38	11	of	of	IN
cord-001142-puj74k7y	38	12	influenza	influenza	NN
cord-001142-puj74k7y	38	13	A	a	DT
cord-001142-puj74k7y	38	14	viruses	virus	NNS
cord-001142-puj74k7y	38	15	in	in	IN
cord-001142-puj74k7y	38	16	DMEM	DMEM	NNP
cord-001142-puj74k7y	38	17	with	with	IN
cord-001142-puj74k7y	38	18	1	1	CD
cord-001142-puj74k7y	38	19	µg	µg	NN
cord-001142-puj74k7y	38	20	/	/	SYM
cord-001142-puj74k7y	38	21	ml	ml	NN
cord-001142-puj74k7y	38	22	of	of	IN
cord-001142-puj74k7y	38	23	L-1-tosylamido-2-phenyl	L-1-tosylamido-2-phenyl	NNP
cord-001142-puj74k7y	38	24	chloromethyl	chloromethyl	NN
cord-001142-puj74k7y	38	25	ketone	ketone	NN
cord-001142-puj74k7y	38	26	TPCK	TPCK	NNP
cord-001142-puj74k7y	38	27	-	-	HYPH
cord-001142-puj74k7y	38	28	trypsin	trypsin	NN
cord-001142-puj74k7y	38	29	(	(	-LRB-
cord-001142-puj74k7y	38	30	Worthington	Worthington	NNP
cord-001142-puj74k7y	38	31	)	)	-RRB-
cord-001142-puj74k7y	38	32	for	for	IN
cord-001142-puj74k7y	38	33	3	3	CD
cord-001142-puj74k7y	38	34	days	day	NNS
cord-001142-puj74k7y	38	35	at	at	IN
cord-001142-puj74k7y	38	36	35	35	CD
cord-001142-puj74k7y	38	37	°	°	NN
cord-001142-puj74k7y	38	38	C	c	NN
cord-001142-puj74k7y	38	39	.	.	.
cord-001142-puj74k7y	39	1	Titration	titration	NN
cord-001142-puj74k7y	39	2	by	by	IN
cord-001142-puj74k7y	39	3	plaque	plaque	NN
cord-001142-puj74k7y	39	4	assay	assay	NN
cord-001142-puj74k7y	39	5	of	of	IN
cord-001142-puj74k7y	39	6	influenza	influenza	NN
cord-001142-puj74k7y	39	7	C	C	NNP
cord-001142-puj74k7y	39	8	viruses	virus	NNS
cord-001142-puj74k7y	39	9	on	on	IN
cord-001142-puj74k7y	39	10	MDCK	MDCK	NNP
cord-001142-puj74k7y	39	11	cells	cell	NNS
cord-001142-puj74k7y	39	12	at	at	IN
cord-001142-puj74k7y	39	13	33	33	CD
cord-001142-puj74k7y	39	14	°	°	NN
cord-001142-puj74k7y	39	15	C	C	NNP
cord-001142-puj74k7y	39	16	was	be	VBD
cord-001142-puj74k7y	39	17	described	describe	VBN
cord-001142-puj74k7y	39	18	previously	previously	RB
cord-001142-puj74k7y	39	19	[	[	-LRB-
cord-001142-puj74k7y	39	20	21	21	CD
cord-001142-puj74k7y	39	21	]	]	-RRB-
cord-001142-puj74k7y	39	22	.	.	.
cord-001142-puj74k7y	40	1	For	for	IN
cord-001142-puj74k7y	40	2	influenza	influenza	NN
cord-001142-puj74k7y	40	3	A	a	DT
cord-001142-puj74k7y	40	4	viruses	virus	NNS
cord-001142-puj74k7y	40	5	,	,	,
cord-001142-puj74k7y	40	6	the	the	DT
cord-001142-puj74k7y	40	7	rescued	rescue	VBN
cord-001142-puj74k7y	40	8	viruses	virus	NNS
cord-001142-puj74k7y	40	9	were	be	VBD
cord-001142-puj74k7y	40	10	plaque	plaque	NN
cord-001142-puj74k7y	40	11	purified	purify	VBN
cord-001142-puj74k7y	40	12	twice	twice	RB
cord-001142-puj74k7y	40	13	on	on	IN
cord-001142-puj74k7y	40	14	MDCK	MDCK	NNP
cord-001142-puj74k7y	40	15	cells	cell	NNS
cord-001142-puj74k7y	40	16	before	before	IN
cord-001142-puj74k7y	40	17	final	final	JJ
cord-001142-puj74k7y	40	18	amplification	amplification	NN
cord-001142-puj74k7y	40	19	on	on	IN
cord-001142-puj74k7y	40	20	MDCK	MDCK	NNP
cord-001142-puj74k7y	40	21	cells	cell	NNS
cord-001142-puj74k7y	40	22	at	at	IN
cord-001142-puj74k7y	40	23	an	an	DT
cord-001142-puj74k7y	40	24	m.o.i	m.o.i	NN
cord-001142-puj74k7y	40	25	.	.	NN
cord-001142-puj74k7y	40	26	of	of	IN
cord-001142-puj74k7y	40	27	0.01	0.01	CD
cord-001142-puj74k7y	40	28	.	.	.
cord-001142-puj74k7y	41	1	Growth	growth	NN
cord-001142-puj74k7y	41	2	kinetics	kinetic	NNS
cord-001142-puj74k7y	41	3	were	be	VBD
cord-001142-puj74k7y	41	4	performed	perform	VBN
cord-001142-puj74k7y	41	5	with	with	IN
cord-001142-puj74k7y	41	6	the	the	DT
cord-001142-puj74k7y	41	7	stock	stock	NN
cord-001142-puj74k7y	41	8	viruses	virus	NNS
cord-001142-puj74k7y	41	9	.	.	.
cord-001142-puj74k7y	42	1	MDCK	MDCK	NNP
cord-001142-puj74k7y	42	2	cells	cell	NNS
cord-001142-puj74k7y	42	3	were	be	VBD
cord-001142-puj74k7y	42	4	infected	infect	VBN
cord-001142-puj74k7y	42	5	at	at	IN
cord-001142-puj74k7y	42	6	an	an	DT
cord-001142-puj74k7y	42	7	m.o.i	m.o.i	NN
cord-001142-puj74k7y	42	8	.	.	NN
cord-001142-puj74k7y	42	9	of	of	IN
cord-001142-puj74k7y	42	10	0.001	0.001	CD
cord-001142-puj74k7y	42	11	in	in	IN
cord-001142-puj74k7y	42	12	DMEM	DMEM	NNP
cord-001142-puj74k7y	42	13	supplemented	supplement	VBN
cord-001142-puj74k7y	42	14	with	with	IN
cord-001142-puj74k7y	42	15	1µg	1µg	CD
cord-001142-puj74k7y	42	16	/	/	SYM
cord-001142-puj74k7y	42	17	mL	ml	NN
cord-001142-puj74k7y	42	18	TPCKtrypsin	TPCKtrypsin	NNP
cord-001142-puj74k7y	42	19	.	.	.
cord-001142-puj74k7y	43	1	The	the	DT
cord-001142-puj74k7y	43	2	rescued	rescue	VBN
cord-001142-puj74k7y	43	3	influenza	influenza	NN
cord-001142-puj74k7y	43	4	C	c	NN
cord-001142-puj74k7y	43	5	viruses	virus	NNS
cord-001142-puj74k7y	43	6	were	be	VBD
cord-001142-puj74k7y	43	7	plaque	plaque	NN
cord-001142-puj74k7y	43	8	purified	purify	VBN
cord-001142-puj74k7y	43	9	on	on	IN
cord-001142-puj74k7y	43	10	MDCK	MDCK	NNP
cord-001142-puj74k7y	43	11	cells	cell	NNS
cord-001142-puj74k7y	43	12	supplemented	supplement	VBN
cord-001142-puj74k7y	43	13	with	with	IN
cord-001142-puj74k7y	43	14	bovine	bovine	JJ
cord-001142-puj74k7y	43	15	brain	brain	NN
cord-001142-puj74k7y	43	16	gangliosides	ganglioside	NNS
cord-001142-puj74k7y	43	17	(	(	-LRB-
cord-001142-puj74k7y	43	18	BBG	BBG	NNP
cord-001142-puj74k7y	43	19	)	)	-RRB-
cord-001142-puj74k7y	43	20	before	before	IN
cord-001142-puj74k7y	43	21	amplification	amplification	NN
cord-001142-puj74k7y	43	22	on	on	IN
cord-001142-puj74k7y	43	23	SK	SK	NNP
cord-001142-puj74k7y	43	24	cells	cell	NNS
cord-001142-puj74k7y	43	25	.	.	.
cord-001142-puj74k7y	44	1	For	for	IN
cord-001142-puj74k7y	44	2	growth	growth	NN
cord-001142-puj74k7y	44	3	kinetics	kinetic	NNS
cord-001142-puj74k7y	44	4	,	,	,
cord-001142-puj74k7y	44	5	SK	SK	NNP
cord-001142-puj74k7y	44	6	cells	cell	NNS
cord-001142-puj74k7y	44	7	were	be	VBD
cord-001142-puj74k7y	44	8	infected	infect	VBN
cord-001142-puj74k7y	44	9	at	at	IN
cord-001142-puj74k7y	44	10	an	an	DT
cord-001142-puj74k7y	44	11	m.o.i	m.o.i	NN
cord-001142-puj74k7y	44	12	.	.	NN
cord-001142-puj74k7y	44	13	of	of	IN
cord-001142-puj74k7y	44	14	0.001	0.001	CD
cord-001142-puj74k7y	44	15	in	in	IN
cord-001142-puj74k7y	44	16	DMEM	DMEM	NNP
cord-001142-puj74k7y	44	17	supplemented	supplement	VBD
cord-001142-puj74k7y	44	18	with	with	IN
cord-001142-puj74k7y	44	19	0.25	0.25	CD
cord-001142-puj74k7y	44	20	µg	µg	NN
cord-001142-puj74k7y	44	21	/	/	SYM
cord-001142-puj74k7y	44	22	mL	mL	NNP
cord-001142-puj74k7y	44	23	TPCK	TPCK	NNP
cord-001142-puj74k7y	44	24	-	-	HYPH
cord-001142-puj74k7y	44	25	trypsin	trypsin	NN
cord-001142-puj74k7y	44	26	.	.	.
cord-001142-puj74k7y	45	1	Supernatants	supernatant	NNS
cord-001142-puj74k7y	45	2	were	be	VBD
cord-001142-puj74k7y	45	3	collected	collect	VBN
cord-001142-puj74k7y	45	4	at	at	IN
cord-001142-puj74k7y	45	5	indicated	indicate	VBN
cord-001142-puj74k7y	45	6	time	time	NN
cord-001142-puj74k7y	45	7	points	point	NNS
cord-001142-puj74k7y	45	8	and	and	CC
cord-001142-puj74k7y	45	9	virus	virus	NN
cord-001142-puj74k7y	45	10	titres	titre	NNS
cord-001142-puj74k7y	45	11	were	be	VBD
cord-001142-puj74k7y	45	12	determined	determine	VBN
cord-001142-puj74k7y	45	13	by	by	IN
cord-001142-puj74k7y	45	14	plaque	plaque	NN
cord-001142-puj74k7y	45	15	assay	assay	NN
cord-001142-puj74k7y	45	16	.	.	.
cord-001142-puj74k7y	46	1	Viral	viral	JJ
cord-001142-puj74k7y	46	2	genomic	genomic	NN
cord-001142-puj74k7y	46	3	RNA	RNA	NNP
cord-001142-puj74k7y	46	4	was	be	VBD
cord-001142-puj74k7y	46	5	extracted	extract	VBN
cord-001142-puj74k7y	46	6	using	use	VBG
cord-001142-puj74k7y	46	7	QIAamp	QIAamp	NNP
cord-001142-puj74k7y	46	8	Viral	Viral	NNP
cord-001142-puj74k7y	46	9	RNA	RNA	NNP
cord-001142-puj74k7y	46	10	Mini	Mini	NNP
cord-001142-puj74k7y	46	11	kit	kit	NN
cord-001142-puj74k7y	46	12	(	(	-LRB-
cord-001142-puj74k7y	46	13	Qiagen	Qiagen	NNP
cord-001142-puj74k7y	46	14	)	)	-RRB-
cord-001142-puj74k7y	46	15	according	accord	VBG
cord-001142-puj74k7y	46	16	to	to	IN
cord-001142-puj74k7y	46	17	the	the	DT
cord-001142-puj74k7y	46	18	manufacturer	manufacturer	NN
cord-001142-puj74k7y	46	19	's	's	POS
cord-001142-puj74k7y	46	20	recommendations	recommendation	NNS
cord-001142-puj74k7y	46	21	and	and	CC
cord-001142-puj74k7y	46	22	cDNA	cdna	NN
cord-001142-puj74k7y	46	23	,	,	,
cord-001142-puj74k7y	46	24	PCR	PCR	NNP
cord-001142-puj74k7y	46	25	and	and	CC
cord-001142-puj74k7y	46	26	sequencing	sequencing	NN
cord-001142-puj74k7y	46	27	were	be	VBD
cord-001142-puj74k7y	46	28	performed	perform	VBN
cord-001142-puj74k7y	46	29	as	as	IN
cord-001142-puj74k7y	46	30	previously	previously	RB
cord-001142-puj74k7y	46	31	described	describe	VBN
cord-001142-puj74k7y	46	32	[	[	-LRB-
cord-001142-puj74k7y	46	33	10	10	CD
cord-001142-puj74k7y	46	34	]	]	-RRB-
cord-001142-puj74k7y	46	35	.	.	.
cord-001142-puj74k7y	47	1	All	all	DT
cord-001142-puj74k7y	47	2	primer	primer	NN
cord-001142-puj74k7y	47	3	sequences	sequence	NNS
cord-001142-puj74k7y	47	4	are	be	VBP
cord-001142-puj74k7y	47	5	available	available	JJ
cord-001142-puj74k7y	47	6	from	from	IN
cord-001142-puj74k7y	47	7	the	the	DT
cord-001142-puj74k7y	47	8	authors	author	NNS
cord-001142-puj74k7y	47	9	upon	upon	IN
cord-001142-puj74k7y	47	10	request	request	NN
cord-001142-puj74k7y	47	11	.	.	.
cord-001142-puj74k7y	48	1	MDCK	MDCK	NNP
cord-001142-puj74k7y	48	2	cells	cell	NNS
cord-001142-puj74k7y	48	3	were	be	VBD
cord-001142-puj74k7y	48	4	infected	infect	VBN
cord-001142-puj74k7y	48	5	at	at	IN
cord-001142-puj74k7y	48	6	an	an	DT
cord-001142-puj74k7y	48	7	m.o.i	m.o.i	NN
cord-001142-puj74k7y	48	8	.	.	NN
cord-001142-puj74k7y	48	9	of	of	IN
cord-001142-puj74k7y	48	10	2	2	CD
cord-001142-puj74k7y	48	11	with	with	IN
cord-001142-puj74k7y	49	1	the	the	DT
cord-001142-puj74k7y	49	2	wild	wild	JJ
cord-001142-puj74k7y	49	3	-	-	HYPH
cord-001142-puj74k7y	49	4	type	type	NN
cord-001142-puj74k7y	49	5	or	or	CC
cord-001142-puj74k7y	49	6	mutated	mutate	VBN
cord-001142-puj74k7y	49	7	influenza	influenza	NN
cord-001142-puj74k7y	49	8	A	a	DT
cord-001142-puj74k7y	49	9	viruses	virus	NNS
cord-001142-puj74k7y	49	10	.	.	.
cord-001142-puj74k7y	50	1	At	at	IN
cord-001142-puj74k7y	50	2	six	six	CD
cord-001142-puj74k7y	50	3	hours	hour	NNS
cord-001142-puj74k7y	50	4	post	post	NN
cord-001142-puj74k7y	50	5	-	-	NN
cord-001142-puj74k7y	50	6	infection	infection	JJ
cord-001142-puj74k7y	50	7	,	,	,
cord-001142-puj74k7y	50	8	total	total	JJ
cord-001142-puj74k7y	50	9	RNAs	rna	NNS
cord-001142-puj74k7y	50	10	were	be	VBD
cord-001142-puj74k7y	50	11	extracted	extract	VBN
cord-001142-puj74k7y	50	12	,	,	,
cord-001142-puj74k7y	50	13	reverse	reverse	VBP
cord-001142-puj74k7y	50	14	transcribed	transcribe	VBN
cord-001142-puj74k7y	50	15	using	use	VBG
cord-001142-puj74k7y	50	16	the	the	DT
cord-001142-puj74k7y	50	17	3	3	CD
cord-001142-puj74k7y	50	18	'	'	POS
cord-001142-puj74k7y	50	19	universal	universal	JJ
cord-001142-puj74k7y	50	20	primer	primer	NN
cord-001142-puj74k7y	50	21	for	for	IN
cord-001142-puj74k7y	50	22	vRNA	vrna	JJ
cord-001142-puj74k7y	50	23	(	(	-LRB-
cord-001142-puj74k7y	50	24	5'AGGGCTCTTCGGCCAGCRAAAGCAGG	5'agggctcttcggccagcraaagcagg	CD
cord-001142-puj74k7y	50	25	)	)	-RRB-
cord-001142-puj74k7y	50	26	or	or	CC
cord-001142-puj74k7y	50	27	oligo	oligo	NNP
cord-001142-puj74k7y	50	28	dT	dT	NNP
cord-001142-puj74k7y	50	29	for	for	IN
cord-001142-puj74k7y	50	30	mRNA	mRNA	NNP
cord-001142-puj74k7y	50	31	,	,	,
cord-001142-puj74k7y	50	32	and	and	CC
cord-001142-puj74k7y	50	33	used	use	VBD
cord-001142-puj74k7y	50	34	as	as	IN
cord-001142-puj74k7y	50	35	a	a	DT
cord-001142-puj74k7y	50	36	template	template	NN
cord-001142-puj74k7y	50	37	for	for	IN
cord-001142-puj74k7y	50	38	quantitative	quantitative	JJ
cord-001142-puj74k7y	50	39	PCR	PCR	NNP
cord-001142-puj74k7y	50	40	(	(	-LRB-
cord-001142-puj74k7y	50	41	qPCR	qPCR	NNP
cord-001142-puj74k7y	50	42	)	)	-RRB-
cord-001142-puj74k7y	50	43	.	.	.
cord-001142-puj74k7y	51	1	Separate	separate	JJ
cord-001142-puj74k7y	51	2	PCRs	pcr	NNS
cord-001142-puj74k7y	51	3	were	be	VBD
cord-001142-puj74k7y	51	4	performed	perform	VBN
cord-001142-puj74k7y	51	5	with	with	IN
cord-001142-puj74k7y	51	6	segment	segment	NN
cord-001142-puj74k7y	51	7	-	-	HYPH
cord-001142-puj74k7y	51	8	specific	specific	JJ
cord-001142-puj74k7y	51	9	primers	primer	NNS
cord-001142-puj74k7y	51	10	designed	design	VBN
cord-001142-puj74k7y	51	11	by	by	IN
cord-001142-puj74k7y	51	12	Marsh	Marsh	NNP
cord-001142-puj74k7y	51	13	et	et	NNP
cord-001142-puj74k7y	51	14	al	al	NNP
cord-001142-puj74k7y	51	15	[	[	-LRB-
cord-001142-puj74k7y	51	16	23	23	CD
cord-001142-puj74k7y	51	17	]	]	-RRB-
cord-001142-puj74k7y	51	18	using	use	VBG
cord-001142-puj74k7y	51	19	of	of	IN
cord-001142-puj74k7y	51	20	a	a	DT
cord-001142-puj74k7y	51	21	LightCycler	LightCycler	NNP
cord-001142-puj74k7y	51	22	480	480	CD
cord-001142-puj74k7y	51	23	(	(	-LRB-
cord-001142-puj74k7y	51	24	Roche	Roche	NNP
cord-001142-puj74k7y	51	25	)	)	-RRB-
cord-001142-puj74k7y	51	26	.	.	.
cord-001142-puj74k7y	52	1	The	the	DT
cord-001142-puj74k7y	52	2	relative	relative	JJ
cord-001142-puj74k7y	52	3	concentrations	concentration	NNS
cord-001142-puj74k7y	52	4	of	of	IN
cord-001142-puj74k7y	52	5	vRNAs	vrna	NNS
cord-001142-puj74k7y	52	6	and	and	CC
cord-001142-puj74k7y	52	7	mRNAs	mRNAs	NNPS
cord-001142-puj74k7y	52	8	were	be	VBD
cord-001142-puj74k7y	52	9	determined	determine	VBN
cord-001142-puj74k7y	52	10	by	by	IN
cord-001142-puj74k7y	52	11	calculating	calculate	VBG
cord-001142-puj74k7y	52	12	mRNA	mRNA	NNP
cord-001142-puj74k7y	52	13	/	/	SYM
cord-001142-puj74k7y	52	14	vRNA	vrna	JJ
cord-001142-puj74k7y	52	15	ratios	ratio	NNS
cord-001142-puj74k7y	52	16	using	use	VBG
cord-001142-puj74k7y	52	17	the	the	DT
cord-001142-puj74k7y	52	18	crossing	crossing	NN
cord-001142-puj74k7y	52	19	point	point	NN
cord-001142-puj74k7y	52	20	values	value	NNS
cord-001142-puj74k7y	52	21	(	(	-LRB-
cord-001142-puj74k7y	52	22	Cp	cp	NN
cord-001142-puj74k7y	52	23	)	)	-RRB-
cord-001142-puj74k7y	52	24	.	.	.
cord-001142-puj74k7y	53	1	As	as	IN
cord-001142-puj74k7y	53	2	control	control	NN
cord-001142-puj74k7y	53	3	,	,	,
cord-001142-puj74k7y	53	4	a	a	DT
cord-001142-puj74k7y	53	5	one	one	CD
cord-001142-puj74k7y	53	6	step	step	NN
cord-001142-puj74k7y	53	7	qRT	qrt	ADD
cord-001142-puj74k7y	53	8	-	-	HYPH
cord-001142-puj74k7y	53	9	PCR	PCR	NNP
cord-001142-puj74k7y	53	10	targeting	target	VBG
cord-001142-puj74k7y	53	11	the	the	DT
cord-001142-puj74k7y	53	12	GAPDH	GAPDH	NNP
cord-001142-puj74k7y	53	13	sequences	sequence	NNS
cord-001142-puj74k7y	53	14	was	be	VBD
cord-001142-puj74k7y	53	15	performed	perform	VBN
cord-001142-puj74k7y	53	16	[	[	-LRB-
cord-001142-puj74k7y	53	17	24	24	CD
cord-001142-puj74k7y	53	18	]	]	-RRB-
cord-001142-puj74k7y	53	19	.	.	.
cord-001142-puj74k7y	54	1	Cells	cell	NNS
cord-001142-puj74k7y	54	2	were	be	VBD
cord-001142-puj74k7y	54	3	solubilised	solubilise	VBN
cord-001142-puj74k7y	54	4	in	in	IN
cord-001142-puj74k7y	54	5	LDS	LDS	NNP
cord-001142-puj74k7y	54	6	sample	sample	NN
cord-001142-puj74k7y	54	7	buffer	buffer	NN
cord-001142-puj74k7y	54	8	(	(	-LRB-
cord-001142-puj74k7y	54	9	Invitrogen	Invitrogen	NNP
cord-001142-puj74k7y	54	10	)	)	-RRB-
cord-001142-puj74k7y	54	11	complemented	complement	VBD
cord-001142-puj74k7y	54	12	with	with	IN
cord-001142-puj74k7y	54	13	β	β	NNP
cord-001142-puj74k7y	54	14	mercaptoethanol	mercaptoethanol	NNP
cord-001142-puj74k7y	54	15	,	,	,
cord-001142-puj74k7y	54	16	and	and	CC
cord-001142-puj74k7y	54	17	proteins	protein	NNS
cord-001142-puj74k7y	54	18	were	be	VBD
cord-001142-puj74k7y	54	19	separated	separate	VBN
cord-001142-puj74k7y	54	20	by	by	IN
cord-001142-puj74k7y	54	21	polyacrylamide	polyacrylamide	NN
cord-001142-puj74k7y	54	22	gel	gel	NN
cord-001142-puj74k7y	54	23	electrophoresis	electrophoresis	NN
cord-001142-puj74k7y	54	24	(	(	-LRB-
cord-001142-puj74k7y	54	25	NUPAGE	NUPAGE	NNP
cord-001142-puj74k7y	54	26	Invitrogen	Invitrogen	NNP
cord-001142-puj74k7y	54	27	)	)	-RRB-
cord-001142-puj74k7y	54	28	followed	follow	VBN
cord-001142-puj74k7y	54	29	by	by	IN
cord-001142-puj74k7y	54	30	western	western	JJ
cord-001142-puj74k7y	54	31	blotting	blotting	NN
cord-001142-puj74k7y	54	32	.	.	.
cord-001142-puj74k7y	55	1	Rabbit	rabbit	NN
cord-001142-puj74k7y	55	2	polyclonal	polyclonal	JJ
cord-001142-puj74k7y	55	3	anti	anti	NNP
cord-001142-puj74k7y	55	4	-	-	NNP
cord-001142-puj74k7y	55	5	NS	NS	NNP
cord-001142-puj74k7y	55	6	antibody	antibody	NN
cord-001142-puj74k7y	55	7	was	be	VBD
cord-001142-puj74k7y	55	8	a	a	DT
cord-001142-puj74k7y	55	9	kind	kind	JJ
cord-001142-puj74k7y	55	10	gift	gift	NN
cord-001142-puj74k7y	55	11	from	from	IN
cord-001142-puj74k7y	55	12	D.	D.	NNP
cord-001142-puj74k7y	55	13	Marc	Marc	NNP
cord-001142-puj74k7y	55	14	(	(	-LRB-
cord-001142-puj74k7y	55	15	INRA	INRA	NNP
cord-001142-puj74k7y	55	16	,	,	,
cord-001142-puj74k7y	55	17	Tours	Tours	NNP
cord-001142-puj74k7y	55	18	,	,	,
cord-001142-puj74k7y	55	19	France	France	NNP
cord-001142-puj74k7y	55	20	)	)	-RRB-
cord-001142-puj74k7y	55	21	.	.	.
cord-001142-puj74k7y	56	1	The	the	DT
cord-001142-puj74k7y	56	2	rabbit	rabbit	NN
cord-001142-puj74k7y	56	3	anti	anti	JJ
cord-001142-puj74k7y	56	4	-	-	NNP
cord-001142-puj74k7y	56	5	PR8	PR8	NNP
cord-001142-puj74k7y	56	6	,	,	,
cord-001142-puj74k7y	56	7	used	use	VBN
cord-001142-puj74k7y	56	8	to	to	TO
cord-001142-puj74k7y	56	9	detect	detect	VB
cord-001142-puj74k7y	56	10	NP	NP	NNP
cord-001142-puj74k7y	56	11	,	,	,
cord-001142-puj74k7y	56	12	was	be	VBD
cord-001142-puj74k7y	56	13	produced	produce	VBN
cord-001142-puj74k7y	56	14	in	in	IN
cord-001142-puj74k7y	56	15	house	house	NN
cord-001142-puj74k7y	56	16	[	[	-LRB-
cord-001142-puj74k7y	56	17	25	25	CD
cord-001142-puj74k7y	56	18	]	]	-RRB-
cord-001142-puj74k7y	56	19	.	.	.
cord-001142-puj74k7y	57	1	Anti	anti	JJ
cord-001142-puj74k7y	57	2	beta	beta	NN
cord-001142-puj74k7y	57	3	-	-	HYPH
cord-001142-puj74k7y	57	4	actin	actin	JJ
cord-001142-puj74k7y	57	5	(	(	-LRB-
cord-001142-puj74k7y	57	6	Abcam	Abcam	NNP
cord-001142-puj74k7y	57	7	,	,	,
cord-001142-puj74k7y	57	8	Cambridge	Cambridge	NNP
cord-001142-puj74k7y	57	9	,	,	,
cord-001142-puj74k7y	57	10	UK	UK	NNP
cord-001142-puj74k7y	57	11	)	)	-RRB-
cord-001142-puj74k7y	57	12	antibody	antibody	NN
cord-001142-puj74k7y	57	13	was	be	VBD
cord-001142-puj74k7y	57	14	used	use	VBN
cord-001142-puj74k7y	57	15	for	for	IN
cord-001142-puj74k7y	57	16	loading	loading	NN
cord-001142-puj74k7y	57	17	controls	control	NNS
cord-001142-puj74k7y	57	18	.	.	.
cord-001142-puj74k7y	58	1	The	the	DT
cord-001142-puj74k7y	58	2	role	role	NN
cord-001142-puj74k7y	58	3	of	of	IN
cord-001142-puj74k7y	58	4	NC	NC	NNP
cord-001142-puj74k7y	58	5	sequences	sequence	NNS
cord-001142-puj74k7y	58	6	in	in	IN
cord-001142-puj74k7y	58	7	type	type	NN
cord-001142-puj74k7y	58	8	specificity	specificity	NN
cord-001142-puj74k7y	58	9	was	be	VBD
cord-001142-puj74k7y	58	10	investigated	investigate	VBN
cord-001142-puj74k7y	58	11	for	for	IN
cord-001142-puj74k7y	58	12	the	the	DT
cord-001142-puj74k7y	58	13	NS	NS	NNP
cord-001142-puj74k7y	58	14	segment	segment	NN
cord-001142-puj74k7y	58	15	of	of	IN
cord-001142-puj74k7y	58	16	the	the	DT
cord-001142-puj74k7y	58	17	most	most	RBS
cord-001142-puj74k7y	58	18	divergent	divergent	JJ
cord-001142-puj74k7y	58	19	type	type	NN
cord-001142-puj74k7y	58	20	A	a	NN
cord-001142-puj74k7y	58	21	and	and	CC
cord-001142-puj74k7y	58	22	C	c	NN
cord-001142-puj74k7y	58	23	influenza	influenza	NN
cord-001142-puj74k7y	58	24	viruses	virus	NNS
cord-001142-puj74k7y	58	25	using	use	VBG
cord-001142-puj74k7y	58	26	reverse	reverse	JJ
cord-001142-puj74k7y	58	27	genetics	genetics	NN
cord-001142-puj74k7y	58	28	systems	system	NNS
cord-001142-puj74k7y	58	29	[	[	-LRB-
cord-001142-puj74k7y	58	30	19	19	CD
cord-001142-puj74k7y	58	31	]	]	-RRB-
cord-001142-puj74k7y	58	32	[	[	-LRB-
cord-001142-puj74k7y	58	33	20	20	CD
cord-001142-puj74k7y	58	34	]	]	-RRB-
cord-001142-puj74k7y	58	35	[	[	-LRB-
cord-001142-puj74k7y	58	36	21	21	CD
cord-001142-puj74k7y	58	37	]	]	-RRB-
cord-001142-puj74k7y	58	38	.	.	.
cord-001142-puj74k7y	59	1	A	a	DT
cord-001142-puj74k7y	59	2	set	set	NN
cord-001142-puj74k7y	59	3	of	of	IN
cord-001142-puj74k7y	59	4	type	type	NN
cord-001142-puj74k7y	59	5	A	a	NN
cord-001142-puj74k7y	59	6	and	and	CC
cord-001142-puj74k7y	59	7	type	type	NN
cord-001142-puj74k7y	59	8	C	c	NN
cord-001142-puj74k7y	59	9	chimeric	chimeric	JJ
cord-001142-puj74k7y	59	10	viruses	virus	NNS
cord-001142-puj74k7y	59	11	was	be	VBD
cord-001142-puj74k7y	59	12	generated	generate	VBN
cord-001142-puj74k7y	59	13	,	,	,
cord-001142-puj74k7y	59	14	in	in	IN
cord-001142-puj74k7y	59	15	which	which	WDT
cord-001142-puj74k7y	59	16	one	one	NN
cord-001142-puj74k7y	59	17	or	or	CC
cord-001142-puj74k7y	59	18	both	both	DT
cord-001142-puj74k7y	59	19	type	type	NN
cord-001142-puj74k7y	59	20	-	-	HYPH
cord-001142-puj74k7y	59	21	specific	specific	JJ
cord-001142-puj74k7y	59	22	extremities	extremity	NNS
cord-001142-puj74k7y	59	23	of	of	IN
cord-001142-puj74k7y	59	24	the	the	DT
cord-001142-puj74k7y	59	25	NS	NS	NNP
cord-001142-puj74k7y	59	26	segment	segment	NN
cord-001142-puj74k7y	59	27	were	be	VBD
cord-001142-puj74k7y	59	28	totally	totally	RB
cord-001142-puj74k7y	59	29	or	or	CC
cord-001142-puj74k7y	59	30	partially	partially	RB
cord-001142-puj74k7y	59	31	replaced	replace	VBN
cord-001142-puj74k7y	59	32	by	by	IN
cord-001142-puj74k7y	59	33	their	-PRON-	PRP$
cord-001142-puj74k7y	59	34	counterpart	counterpart	NN
cord-001142-puj74k7y	59	35	from	from	IN
cord-001142-puj74k7y	59	36	type	type	NN
cord-001142-puj74k7y	59	37	C	c	NN
cord-001142-puj74k7y	59	38	or	or	CC
cord-001142-puj74k7y	59	39	type	type	VB
cord-001142-puj74k7y	59	40	A	a	DT
cord-001142-puj74k7y	59	41	virus	virus	NN
cord-001142-puj74k7y	59	42	,	,	,
cord-001142-puj74k7y	59	43	respectively	respectively	RB
cord-001142-puj74k7y	59	44	(	(	-LRB-
cord-001142-puj74k7y	59	45	Figure	figure	NN
cord-001142-puj74k7y	59	46	1	1	CD
cord-001142-puj74k7y	59	47	)	)	-RRB-
cord-001142-puj74k7y	59	48	.	.	.
cord-001142-puj74k7y	60	1	In	in	IN
cord-001142-puj74k7y	60	2	a	a	DT
cord-001142-puj74k7y	60	3	type	type	NN
cord-001142-puj74k7y	60	4	C	c	NN
cord-001142-puj74k7y	60	5	backbone	backbone	NN
cord-001142-puj74k7y	60	6	,	,	,
cord-001142-puj74k7y	60	7	no	no	DT
cord-001142-puj74k7y	60	8	virus	virus	NN
cord-001142-puj74k7y	60	9	was	be	VBD
cord-001142-puj74k7y	60	10	rescued	rescue	VBN
cord-001142-puj74k7y	60	11	when	when	WRB
cord-001142-puj74k7y	60	12	one	one	CD
cord-001142-puj74k7y	60	13	or	or	CC
cord-001142-puj74k7y	60	14	both	both	DT
cord-001142-puj74k7y	60	15	type	type	NN
cord-001142-puj74k7y	61	1	A	a	DT
cord-001142-puj74k7y	61	2	extremities	extremity	NNS
cord-001142-puj74k7y	61	3	were	be	VBD
cord-001142-puj74k7y	61	4	introduced	introduce	VBN
cord-001142-puj74k7y	61	5	,	,	,
cord-001142-puj74k7y	61	6	and	and	CC
cord-001142-puj74k7y	61	7	only	only	RB
cord-001142-puj74k7y	61	8	the	the	DT
cord-001142-puj74k7y	61	9	wild	wild	JJ
cord-001142-puj74k7y	61	10	-	-	HYPH
cord-001142-puj74k7y	61	11	type	type	NN
cord-001142-puj74k7y	61	12	5'C/3'C	5'c/3'c	NN
cord-001142-puj74k7y	61	13	virus	virus	NN
cord-001142-puj74k7y	61	14	was	be	VBD
cord-001142-puj74k7y	61	15	recovered	recover	VBN
cord-001142-puj74k7y	61	16	(	(	-LRB-
cord-001142-puj74k7y	61	17	Figure	figure	NN
cord-001142-puj74k7y	61	18	1	1	CD
cord-001142-puj74k7y	61	19	)	)	-RRB-
cord-001142-puj74k7y	61	20	.	.	.
cord-001142-puj74k7y	62	1	In	in	IN
cord-001142-puj74k7y	62	2	a	a	DT
cord-001142-puj74k7y	62	3	type	type	NN
cord-001142-puj74k7y	62	4	A	a	DT
cord-001142-puj74k7y	62	5	backbone	backbone	NN
cord-001142-puj74k7y	62	6	(	(	-LRB-
cord-001142-puj74k7y	62	7	Figure	figure	NN
cord-001142-puj74k7y	62	8	1	1	CD
cord-001142-puj74k7y	62	9	)	)	-RRB-
cord-001142-puj74k7y	62	10	,	,	,
cord-001142-puj74k7y	62	11	no	no	DT
cord-001142-puj74k7y	62	12	virus	virus	NN
cord-001142-puj74k7y	62	13	harbouring	harbour	VBG
cord-001142-puj74k7y	62	14	the	the	DT
cord-001142-puj74k7y	62	15	type	type	NN
cord-001142-puj74k7y	62	16	C	C	NNP
cord-001142-puj74k7y	62	17	5'end	5'end	CD
cord-001142-puj74k7y	62	18	(	(	-LRB-
cord-001142-puj74k7y	62	19	5'C/3'C	5'c/3'c	NN
cord-001142-puj74k7y	62	20	and	and	CC
cord-001142-puj74k7y	62	21	5'C/3'A	5'c/3'a	NN
cord-001142-puj74k7y	62	22	)	)	-RRB-
cord-001142-puj74k7y	62	23	was	be	VBD
cord-001142-puj74k7y	62	24	rescued	rescue	VBN
cord-001142-puj74k7y	62	25	.	.	.
cord-001142-puj74k7y	63	1	Only	only	RB
cord-001142-puj74k7y	63	2	viruses	virus	NNS
cord-001142-puj74k7y	63	3	with	with	IN
cord-001142-puj74k7y	63	4	a	a	DT
cord-001142-puj74k7y	63	5	type	type	NN
cord-001142-puj74k7y	64	1	A	a	DT
cord-001142-puj74k7y	64	2	5	5	CD
cord-001142-puj74k7y	64	3	'	'	''
cord-001142-puj74k7y	64	4	end	end	NN
cord-001142-puj74k7y	64	5	(	(	-LRB-
cord-001142-puj74k7y	64	6	5'A/3'A	5'a/3'a	NN
cord-001142-puj74k7y	64	7	and	and	CC
cord-001142-puj74k7y	64	8	5'A/3'C	5'a/3'c	LS
cord-001142-puj74k7y	64	9	)	)	-RRB-
cord-001142-puj74k7y	64	10	could	could	MD
cord-001142-puj74k7y	64	11	be	be	VB
cord-001142-puj74k7y	64	12	rescued	rescue	VBN
cord-001142-puj74k7y	64	13	,	,	,
cord-001142-puj74k7y	64	14	but	but	CC
cord-001142-puj74k7y	64	15	a	a	DT
cord-001142-puj74k7y	64	16	C	c	NN
cord-001142-puj74k7y	64	17	-	-	HYPH
cord-001142-puj74k7y	64	18	to	to	IN
cord-001142-puj74k7y	64	19	-	-	HYPH
cord-001142-puj74k7y	64	20	U	u	NN
cord-001142-puj74k7y	64	21	mutation	mutation	NN
cord-001142-puj74k7y	64	22	in	in	IN
cord-001142-puj74k7y	64	23	the	the	DT
cord-001142-puj74k7y	64	24	3'C	3'c	CD
cord-001142-puj74k7y	64	25	proximal	proximal	JJ
cord-001142-puj74k7y	64	26	sequence	sequence	NN
cord-001142-puj74k7y	64	27	was	be	VBD
cord-001142-puj74k7y	64	28	systematically	systematically	RB
cord-001142-puj74k7y	64	29	observed	observe	VBN
cord-001142-puj74k7y	64	30	at	at	IN
cord-001142-puj74k7y	64	31	nucleotide	nucleotide	JJ
cord-001142-puj74k7y	64	32	5	5	CD
cord-001142-puj74k7y	64	33	(	(	-LRB-
cord-001142-puj74k7y	64	34	5'A/3'C(C5U	5'a/3'c(c5u	CD
cord-001142-puj74k7y	64	35	)	)	-RRB-
cord-001142-puj74k7y	64	36	)	)	-RRB-
cord-001142-puj74k7y	64	37	.	.	.
cord-001142-puj74k7y	65	1	Introduction	introduction	NN
cord-001142-puj74k7y	65	2	of	of	IN
cord-001142-puj74k7y	65	3	this	this	DT
cord-001142-puj74k7y	65	4	reverse	reverse	JJ
cord-001142-puj74k7y	65	5	substitution	substitution	NN
cord-001142-puj74k7y	65	6	into	into	IN
cord-001142-puj74k7y	65	7	the	the	DT
cord-001142-puj74k7y	65	8	wild	wild	JJ
cord-001142-puj74k7y	65	9	-	-	HYPH
cord-001142-puj74k7y	65	10	type	type	NN
cord-001142-puj74k7y	65	11	5'A/3'A	5'a/3'a	NN
cord-001142-puj74k7y	65	12	construct	construct	VB
cord-001142-puj74k7y	65	13	(	(	-LRB-
cord-001142-puj74k7y	65	14	to	to	TO
cord-001142-puj74k7y	65	15	generate	generate	VB
cord-001142-puj74k7y	65	16	5'A/3'A(U5C	5'a/3'a(u5c	CD
cord-001142-puj74k7y	65	17	)	)	-RRB-
cord-001142-puj74k7y	65	18	)	)	-RRB-
cord-001142-puj74k7y	66	1	systematically	systematically	RB
cord-001142-puj74k7y	66	2	led	lead	VBN
cord-001142-puj74k7y	66	3	to	to	IN
cord-001142-puj74k7y	66	4	a	a	DT
cord-001142-puj74k7y	66	5	wild	wild	JJ
cord-001142-puj74k7y	66	6	-	-	HYPH
cord-001142-puj74k7y	66	7	type	type	NN
cord-001142-puj74k7y	66	8	uracil	uracil	NN
cord-001142-puj74k7y	66	9	reversion	reversion	NN
cord-001142-puj74k7y	66	10	,	,	,
cord-001142-puj74k7y	66	11	confirming	confirm	VBG
cord-001142-puj74k7y	66	12	the	the	DT
cord-001142-puj74k7y	66	13	importance	importance	NN
cord-001142-puj74k7y	66	14	of	of	IN
cord-001142-puj74k7y	66	15	this	this	DT
cord-001142-puj74k7y	66	16	position	position	NN
cord-001142-puj74k7y	66	17	.	.	.
cord-001142-puj74k7y	67	1	Minigenome	Minigenome	NNP
cord-001142-puj74k7y	67	2	experiments	experiment	NNS
cord-001142-puj74k7y	67	3	previously	previously	RB
cord-001142-puj74k7y	67	4	showed	show	VBD
cord-001142-puj74k7y	67	5	that	that	IN
cord-001142-puj74k7y	67	6	this	this	DT
cord-001142-puj74k7y	67	7	position	position	NN
cord-001142-puj74k7y	67	8	did	do	VBD
cord-001142-puj74k7y	67	9	not	not	RB
cord-001142-puj74k7y	67	10	modify	modify	VB
cord-001142-puj74k7y	67	11	the	the	DT
cord-001142-puj74k7y	67	12	transcription	transcription	NN
cord-001142-puj74k7y	67	13	/	/	SYM
cord-001142-puj74k7y	67	14	replication	replication	NN
cord-001142-puj74k7y	67	15	processes	process	NNS
cord-001142-puj74k7y	67	16	[	[	-LRB-
cord-001142-puj74k7y	67	17	10	10	CD
cord-001142-puj74k7y	67	18	]	]	-RRB-
cord-001142-puj74k7y	67	19	.	.	.
cord-001142-puj74k7y	68	1	Interestingly	interestingly	RB
cord-001142-puj74k7y	68	2	,	,	,
cord-001142-puj74k7y	68	3	this	this	DT
cord-001142-puj74k7y	68	4	mutation	mutation	NN
cord-001142-puj74k7y	68	5	in	in	IN
cord-001142-puj74k7y	68	6	5'A/3'C	5'a/3'c	CD
cord-001142-puj74k7y	68	7	,	,	,
cord-001142-puj74k7y	68	8	leading	lead	VBG
cord-001142-puj74k7y	68	9	to	to	IN
cord-001142-puj74k7y	68	10	5'A/3'C(C5U	5'a/3'c(c5u	CD
cord-001142-puj74k7y	68	11	)	)	-RRB-
cord-001142-puj74k7y	68	12	,	,	,
cord-001142-puj74k7y	68	13	restored	restore	VBD
cord-001142-puj74k7y	68	14	a	a	DT
cord-001142-puj74k7y	68	15	proximal	proximal	JJ
cord-001142-puj74k7y	68	16	panhandle	panhandle	NN
cord-001142-puj74k7y	68	17	sequence	sequence	NN
cord-001142-puj74k7y	68	18	identical	identical	JJ
cord-001142-puj74k7y	68	19	to	to	IN
cord-001142-puj74k7y	68	20	that	that	DT
cord-001142-puj74k7y	68	21	of	of	IN
cord-001142-puj74k7y	68	22	the	the	DT
cord-001142-puj74k7y	68	23	wild	wild	JJ
cord-001142-puj74k7y	68	24	-	-	HYPH
cord-001142-puj74k7y	68	25	type	type	NN
cord-001142-puj74k7y	68	26	5'A/3'A.	5'a/3'a.	CD
cord-001142-puj74k7y	68	27	To	to	TO
cord-001142-puj74k7y	68	28	demonstrate	demonstrate	VB
cord-001142-puj74k7y	68	29	the	the	DT
cord-001142-puj74k7y	68	30	necessity	necessity	NN
cord-001142-puj74k7y	68	31	of	of	IN
cord-001142-puj74k7y	68	32	the	the	DT
cord-001142-puj74k7y	68	33	type	type	NN
cord-001142-puj74k7y	68	34	specific	specific	JJ
cord-001142-puj74k7y	68	35	proximal	proximal	JJ
cord-001142-puj74k7y	68	36	panhandles	panhandle	NNS
cord-001142-puj74k7y	68	37	to	to	TO
cord-001142-puj74k7y	68	38	recover	recover	VB
cord-001142-puj74k7y	68	39	viruses	virus	NNS
cord-001142-puj74k7y	68	40	,	,	,
cord-001142-puj74k7y	68	41	chimeric	chimeric	JJ
cord-001142-puj74k7y	68	42	heterotypic	heterotypic	NNP
cord-001142-puj74k7y	68	43	ends	end	NNS
cord-001142-puj74k7y	68	44	were	be	VBD
cord-001142-puj74k7y	68	45	created	create	VBN
cord-001142-puj74k7y	68	46	to	to	TO
cord-001142-puj74k7y	68	47	include	include	VB
cord-001142-puj74k7y	68	48	proximal	proximal	JJ
cord-001142-puj74k7y	68	49	panhandles	panhandles	NNP
cord-001142-puj74k7y	68	50	homotypic	homotypic	NNP
cord-001142-puj74k7y	68	51	of	of	IN
cord-001142-puj74k7y	68	52	the	the	DT
cord-001142-puj74k7y	68	53	reverse	reverse	JJ
cord-001142-puj74k7y	68	54	genetics	genetics	NN
cord-001142-puj74k7y	68	55	backbone	backbone	NN
cord-001142-puj74k7y	68	56	.	.	.
cord-001142-puj74k7y	69	1	Both	both	DT
cord-001142-puj74k7y	69	2	type	type	NN
cord-001142-puj74k7y	69	3	A	a	NN
cord-001142-puj74k7y	69	4	and	and	CC
cord-001142-puj74k7y	69	5	C	c	NN
cord-001142-puj74k7y	69	6	viruses	virus	NNS
cord-001142-puj74k7y	69	7	with	with	IN
cord-001142-puj74k7y	69	8	5	5	CD
cord-001142-puj74k7y	69	9	'	'	''
cord-001142-puj74k7y	69	10	and	and	CC
cord-001142-puj74k7y	69	11	3	3	CD
cord-001142-puj74k7y	69	12	'	'	''
cord-001142-puj74k7y	69	13	modified	modify	VBN
cord-001142-puj74k7y	69	14	NS	NS	NNP
cord-001142-puj74k7y	69	15	ends	end	VBZ
cord-001142-puj74k7y	69	16	(	(	-LRB-
cord-001142-puj74k7y	69	17	5'C/3'C	5'c/3'c	NN
cord-001142-puj74k7y	69	18	-	-	:
cord-001142-puj74k7y	69	19	proxA	proxa	JJ
cord-001142-puj74k7y	69	20	and	and	CC
cord-001142-puj74k7y	69	21	5'A/3'A	5'a/3'a	NN
cord-001142-puj74k7y	69	22	-	-	:
cord-001142-puj74k7y	69	23	proxC	proxc	JJ
cord-001142-puj74k7y	69	24	,	,	,
cord-001142-puj74k7y	69	25	respectively	respectively	RB
cord-001142-puj74k7y	69	26	)	)	-RRB-
cord-001142-puj74k7y	69	27	were	be	VBD
cord-001142-puj74k7y	69	28	rescued	rescue	VBN
cord-001142-puj74k7y	69	29	as	as	RB
cord-001142-puj74k7y	69	30	efficiently	efficiently	RB
cord-001142-puj74k7y	69	31	as	as	IN
cord-001142-puj74k7y	69	32	wild	wild	JJ
cord-001142-puj74k7y	69	33	-	-	HYPH
cord-001142-puj74k7y	69	34	type	type	NN
cord-001142-puj74k7y	69	35	viruses	virus	NNS
cord-001142-puj74k7y	69	36	(	(	-LRB-
cord-001142-puj74k7y	69	37	Figure	figure	NN
cord-001142-puj74k7y	69	38	1	1	CD
cord-001142-puj74k7y	69	39	)	)	-RRB-
cord-001142-puj74k7y	69	40	.	.	.
cord-001142-puj74k7y	70	1	The	the	DT
cord-001142-puj74k7y	70	2	fact	fact	NN
cord-001142-puj74k7y	70	3	that	that	IN
cord-001142-puj74k7y	70	4	the	the	DT
cord-001142-puj74k7y	70	5	proximal	proximal	JJ
cord-001142-puj74k7y	70	6	panhandle	panhandle	NN
cord-001142-puj74k7y	70	7	of	of	IN
cord-001142-puj74k7y	70	8	the	the	DT
cord-001142-puj74k7y	70	9	unsuccessful	unsuccessful	JJ
cord-001142-puj74k7y	70	10	constructs	construct	NNS
cord-001142-puj74k7y	70	11	is	be	VBZ
cord-001142-puj74k7y	70	12	not	not	RB
cord-001142-puj74k7y	70	13	homotypic	homotypic	JJ
cord-001142-puj74k7y	70	14	strongly	strongly	RB
cord-001142-puj74k7y	70	15	suggested	suggest	VBD
cord-001142-puj74k7y	70	16	that	that	IN
cord-001142-puj74k7y	70	17	a	a	DT
cord-001142-puj74k7y	70	18	homotypic	homotypic	JJ
cord-001142-puj74k7y	70	19	proximal	proximal	NNP
cord-001142-puj74k7y	70	20	panhandle	panhandle	NNP
cord-001142-puj74k7y	70	21	is	be	VBZ
cord-001142-puj74k7y	70	22	required	require	VBN
cord-001142-puj74k7y	70	23	.	.	.
cord-001142-puj74k7y	71	1	All	all	DT
cord-001142-puj74k7y	71	2	rescued	rescue	VBN
cord-001142-puj74k7y	71	3	type	type	NN
cord-001142-puj74k7y	71	4	C	c	NN
cord-001142-puj74k7y	71	5	and	and	CC
cord-001142-puj74k7y	71	6	A	a	DT
cord-001142-puj74k7y	71	7	viruses	virus	NNS
cord-001142-puj74k7y	71	8	were	be	VBD
cord-001142-puj74k7y	71	9	plaque	plaque	NN
cord-001142-puj74k7y	71	10	-	-	HYPH
cord-001142-puj74k7y	71	11	purified	purify	VBN
cord-001142-puj74k7y	71	12	and	and	CC
cord-001142-puj74k7y	71	13	subsequently	subsequently	RB
cord-001142-puj74k7y	71	14	amplified	amplify	VBN
cord-001142-puj74k7y	71	15	in	in	IN
cord-001142-puj74k7y	71	16	SK	SK	NNP
cord-001142-puj74k7y	71	17	and	and	CC
cord-001142-puj74k7y	71	18	MDCK	MDCK	NNP
cord-001142-puj74k7y	71	19	cells	cell	NNS
cord-001142-puj74k7y	71	20	,	,	,
cord-001142-puj74k7y	71	21	respectively	respectively	RB
cord-001142-puj74k7y	71	22	.	.	.
cord-001142-puj74k7y	72	1	Kinetics	kinetics	NN
cord-001142-puj74k7y	72	2	assays	assay	NNS
cord-001142-puj74k7y	72	3	at	at	IN
cord-001142-puj74k7y	72	4	low	low	JJ
cord-001142-puj74k7y	72	5	multiplicity	multiplicity	NN
cord-001142-puj74k7y	72	6	-	-	HYPH
cord-001142-puj74k7y	72	7	of	of	IN
cord-001142-puj74k7y	72	8	-	-	HYPH
cord-001142-puj74k7y	72	9	infection	infection	NN
cord-001142-puj74k7y	72	10	(	(	-LRB-
cord-001142-puj74k7y	72	11	m.o.i	m.o.i	NNP
cord-001142-puj74k7y	72	12	.	.	.
cord-001142-puj74k7y	72	13	)	)	-RRB-
cord-001142-puj74k7y	72	14	were	be	VBD
cord-001142-puj74k7y	72	15	then	then	RB
cord-001142-puj74k7y	72	16	performed	perform	VBN
cord-001142-puj74k7y	72	17	in	in	IN
cord-001142-puj74k7y	72	18	the	the	DT
cord-001142-puj74k7y	72	19	respective	respective	JJ
cord-001142-puj74k7y	72	20	cell	cell	NN
cord-001142-puj74k7y	72	21	types	type	NNS
cord-001142-puj74k7y	72	22	.	.	.
cord-001142-puj74k7y	73	1	No	no	DT
cord-001142-puj74k7y	73	2	difference	difference	NN
cord-001142-puj74k7y	73	3	was	be	VBD
cord-001142-puj74k7y	73	4	observed	observe	VBN
cord-001142-puj74k7y	73	5	for	for	IN
cord-001142-puj74k7y	73	6	type	type	NN
cord-001142-puj74k7y	73	7	C	c	NN
cord-001142-puj74k7y	73	8	viruses	virus	NNS
cord-001142-puj74k7y	73	9	;	;	:
cord-001142-puj74k7y	73	10	wild	wild	JJ
cord-001142-puj74k7y	73	11	-	-	HYPH
cord-001142-puj74k7y	73	12	type	type	NN
cord-001142-puj74k7y	73	13	and	and	CC
cord-001142-puj74k7y	73	14	5'A/3'A	5'a/3'a	NN
cord-001142-puj74k7y	73	15	-	-	:
cord-001142-puj74k7y	73	16	proxC	proxC	NNP
cord-001142-puj74k7y	73	17	grew	grow	VBD
cord-001142-puj74k7y	73	18	with	with	IN
cord-001142-puj74k7y	73	19	similar	similar	JJ
cord-001142-puj74k7y	73	20	efficiencies	efficiency	NNS
cord-001142-puj74k7y	73	21	(	(	-LRB-
cord-001142-puj74k7y	73	22	Figure	figure	NN
cord-001142-puj74k7y	73	23	2A	2a	CD
cord-001142-puj74k7y	73	24	)	)	-RRB-
cord-001142-puj74k7y	73	25	.	.	.
cord-001142-puj74k7y	74	1	The	the	DT
cord-001142-puj74k7y	74	2	three	three	CD
cord-001142-puj74k7y	74	3	rescued	rescue	VBN
cord-001142-puj74k7y	74	4	type	type	NN
cord-001142-puj74k7y	74	5	A	a	NN
cord-001142-puj74k7y	74	6	[	[	-LRB-
cord-001142-puj74k7y	74	7	20	20	CD
cord-001142-puj74k7y	74	8	,	,	,
cord-001142-puj74k7y	74	9	21	21	CD
cord-001142-puj74k7y	74	10	]	]	-RRB-
cord-001142-puj74k7y	74	11	.	.	.
cord-001142-puj74k7y	75	1	The	the	DT
cord-001142-puj74k7y	75	2	introduced	introduce	VBN
cord-001142-puj74k7y	75	3	mutation	mutation	NN
cord-001142-puj74k7y	75	4	in	in	IN
cord-001142-puj74k7y	75	5	5'A/3'A(U5C	5'a/3'a(u5c	CD
cord-001142-puj74k7y	75	6	)	)	-RRB-
cord-001142-puj74k7y	75	7	is	be	VBZ
cord-001142-puj74k7y	75	8	underlined	underline	VBN
cord-001142-puj74k7y	75	9	.	.	.
cord-001142-puj74k7y	76	1	The	the	DT
cord-001142-puj74k7y	76	2	sequence	sequence	NN
cord-001142-puj74k7y	76	3	of	of	IN
cord-001142-puj74k7y	76	4	both	both	DT
cord-001142-puj74k7y	76	5	ends	end	NNS
cord-001142-puj74k7y	76	6	of	of	IN
cord-001142-puj74k7y	76	7	each	each	DT
cord-001142-puj74k7y	76	8	segment	segment	NN
cord-001142-puj74k7y	76	9	of	of	IN
cord-001142-puj74k7y	76	10	each	each	DT
cord-001142-puj74k7y	76	11	rescued	rescue	VBN
cord-001142-puj74k7y	76	12	virus	virus	NN
cord-001142-puj74k7y	76	13	was	be	VBD
cord-001142-puj74k7y	76	14	verified	verify	VBN
cord-001142-puj74k7y	76	15	as	as	IN
cord-001142-puj74k7y	76	16	described	describe	VBN
cord-001142-puj74k7y	76	17	[	[	-LRB-
cord-001142-puj74k7y	76	18	10	10	CD
cord-001142-puj74k7y	76	19	]	]	-RRB-
cord-001142-puj74k7y	76	20	,	,	,
cord-001142-puj74k7y	76	21	and	and	CC
cord-001142-puj74k7y	76	22	no	no	DT
cord-001142-puj74k7y	76	23	mutation	mutation	NN
cord-001142-puj74k7y	76	24	was	be	VBD
cord-001142-puj74k7y	76	25	detected	detect	VBN
cord-001142-puj74k7y	76	26	except	except	IN
cord-001142-puj74k7y	76	27	at	at	IN
cord-001142-puj74k7y	76	28	position	position	NN
cord-001142-puj74k7y	76	29	5	5	CD
cord-001142-puj74k7y	76	30	in	in	IN
cord-001142-puj74k7y	76	31	the	the	DT
cord-001142-puj74k7y	76	32	3	3	CD
cord-001142-puj74k7y	76	33	'	'	NN
cord-001142-puj74k7y	76	34	end	end	NN
cord-001142-puj74k7y	76	35	for	for	IN
cord-001142-puj74k7y	76	36	5'A/3'C	5'a/3'c	CD
cord-001142-puj74k7y	76	37	and	and	CC
cord-001142-puj74k7y	76	38	5'A/3'A(U5C	5'a/3'a(u5c	CD
cord-001142-puj74k7y	76	39	)	)	-RRB-
cord-001142-puj74k7y	76	40	yielding	yield	VBG
cord-001142-puj74k7y	76	41	viruses	virus	NNS
cord-001142-puj74k7y	76	42	5'A/	5'a/	CD
cord-001142-puj74k7y	76	43	3'C(C5U	3'c(c5u	CD
cord-001142-puj74k7y	76	44	)	)	-RRB-
cord-001142-puj74k7y	76	45	and	and	CC
cord-001142-puj74k7y	76	46	5'A/3'A	5'a/3'a	NN
cord-001142-puj74k7y	76	47	,	,	,
cord-001142-puj74k7y	76	48	respectively	respectively	RB
cord-001142-puj74k7y	76	49	(	(	-LRB-
cord-001142-puj74k7y	76	50	indicated	indicate	VBN
cord-001142-puj74k7y	76	51	by	by	IN
cord-001142-puj74k7y	76	52	arrows	arrow	NNS
cord-001142-puj74k7y	76	53	)	)	-RRB-
cord-001142-puj74k7y	76	54	.	.	.
cord-001142-puj74k7y	77	1	In	in	IN
cord-001142-puj74k7y	77	2	constructs	construct	NNS
cord-001142-puj74k7y	77	3	5'A/3'A	5'a/3'a	NN
cord-001142-puj74k7y	77	4	-	-	:
cord-001142-puj74k7y	77	5	proxC	proxC	NNP
cord-001142-puj74k7y	77	6	in	in	IN
cord-001142-puj74k7y	77	7	the	the	DT
cord-001142-puj74k7y	77	8	type	type	NN
cord-001142-puj74k7y	77	9	C	c	NN
cord-001142-puj74k7y	77	10	system	system	NN
cord-001142-puj74k7y	77	11	and	and	CC
cord-001142-puj74k7y	77	12	5'C/3'C	5'c/3'c	NN
cord-001142-puj74k7y	77	13	-	-	:
cord-001142-puj74k7y	77	14	proxA	proxA	NNP
cord-001142-puj74k7y	77	15	in	in	IN
cord-001142-puj74k7y	77	16	the	the	DT
cord-001142-puj74k7y	77	17	type	type	NN
cord-001142-puj74k7y	77	18	A	a	NN
cord-001142-puj74k7y	77	19	system	system	NN
cord-001142-puj74k7y	77	20	,	,	,
cord-001142-puj74k7y	77	21	only	only	RB
cord-001142-puj74k7y	77	22	the	the	DT
cord-001142-puj74k7y	77	23	distal	distal	JJ
cord-001142-puj74k7y	77	24	panhandle	panhandle	NN
cord-001142-puj74k7y	77	25	was	be	VBD
cord-001142-puj74k7y	77	26	modified	modify	VBN
cord-001142-puj74k7y	77	27	,	,	,
cord-001142-puj74k7y	77	28	but	but	CC
cord-001142-puj74k7y	77	29	the	the	DT
cord-001142-puj74k7y	77	30	homotypic	homotypic	NNP
cord-001142-puj74k7y	77	31	proximal	proximal	NNP
cord-001142-puj74k7y	77	32	panhandle	panhandle	NNP
cord-001142-puj74k7y	77	33	was	be	VBD
cord-001142-puj74k7y	77	34	conserved	conserve	VBN
cord-001142-puj74k7y	77	35	.	.	.
cord-001142-puj74k7y	78	1	The	the	DT
cord-001142-puj74k7y	78	2	energy	energy	NN
cord-001142-puj74k7y	78	3	barriers	barrier	NNS
cord-001142-puj74k7y	78	4	of	of	IN
cord-001142-puj74k7y	78	5	the	the	DT
cord-001142-puj74k7y	78	6	canonical	canonical	JJ
cord-001142-puj74k7y	78	7	pairs	pair	NNS
cord-001142-puj74k7y	78	8	C	c	NN
cord-001142-puj74k7y	78	9	:	:	:
cord-001142-puj74k7y	78	10	G	g	NN
cord-001142-puj74k7y	78	11	and	and	CC
cord-001142-puj74k7y	78	12	U	u	NN
cord-001142-puj74k7y	78	13	:	:	:
cord-001142-puj74k7y	78	14	A	a	NN
cord-001142-puj74k7y	78	15	,	,	,
cord-001142-puj74k7y	78	16	and	and	CC
cord-001142-puj74k7y	78	17	of	of	IN
cord-001142-puj74k7y	78	18	the	the	DT
cord-001142-puj74k7y	78	19	wobble	wobble	NN
cord-001142-puj74k7y	78	20	base	base	NN
cord-001142-puj74k7y	78	21	pair	pair	NN
cord-001142-puj74k7y	78	22	G	g	NN
cord-001142-puj74k7y	78	23	:	:	:
cord-001142-puj74k7y	78	24	U	U	NNP
cord-001142-puj74k7y	78	25	(	(	-LRB-
cord-001142-puj74k7y	78	26	represented	represent	VBN
cord-001142-puj74k7y	78	27	by	by	IN
cord-001142-puj74k7y	78	28	a	a	DT
cord-001142-puj74k7y	78	29	black	black	JJ
cord-001142-puj74k7y	78	30	dot	dot	NN
cord-001142-puj74k7y	78	31	)	)	-RRB-
cord-001142-puj74k7y	78	32	were	be	VBD
cord-001142-puj74k7y	78	33	described	describe	VBN
cord-001142-puj74k7y	78	34	by	by	IN
cord-001142-puj74k7y	78	35	Vendeix	Vendeix	NNP
cord-001142-puj74k7y	78	36	et	et	NNP
cord-001142-puj74k7y	78	37	al	al	NNP
cord-001142-puj74k7y	78	38	[	[	-LRB-
cord-001142-puj74k7y	78	39	34	34	CD
cord-001142-puj74k7y	78	40	]	]	-RRB-
cord-001142-puj74k7y	78	41	and	and	CC
cord-001142-puj74k7y	78	42	were	be	VBD
cord-001142-puj74k7y	78	43	used	use	VBN
cord-001142-puj74k7y	78	44	to	to	TO
cord-001142-puj74k7y	78	45	calculate	calculate	VB
cord-001142-puj74k7y	78	46	a	a	DT
cord-001142-puj74k7y	78	47	score	score	NN
cord-001142-puj74k7y	78	48	to	to	TO
cord-001142-puj74k7y	78	49	evaluate	evaluate	VB
cord-001142-puj74k7y	78	50	the	the	DT
cord-001142-puj74k7y	78	51	panhandle	panhandle	NN
cord-001142-puj74k7y	78	52	strength	strength	NN
cord-001142-puj74k7y	78	53	.	.	.
cord-001142-puj74k7y	79	1	The	the	DT
cord-001142-puj74k7y	79	2	proximal	proximal	JJ
cord-001142-puj74k7y	79	3	panhandle	panhandle	NN
cord-001142-puj74k7y	79	4	consists	consist	VBZ
cord-001142-puj74k7y	79	5	of	of	IN
cord-001142-puj74k7y	79	6	9	9	CD
cord-001142-puj74k7y	79	7	potential	potential	JJ
cord-001142-puj74k7y	79	8	basepairs	basepair	NNS
cord-001142-puj74k7y	79	9	,	,	,
cord-001142-puj74k7y	79	10	when	when	WRB
cord-001142-puj74k7y	79	11	the	the	DT
cord-001142-puj74k7y	79	12	distal	distal	JJ
cord-001142-puj74k7y	79	13	panhandle	panhandle	NN
cord-001142-puj74k7y	79	14	was	be	VBD
cord-001142-puj74k7y	79	15	defined	define	VBN
cord-001142-puj74k7y	79	16	to	to	TO
cord-001142-puj74k7y	79	17	include	include	VB
cord-001142-puj74k7y	79	18	the	the	DT
cord-001142-puj74k7y	79	19	potential	potential	JJ
cord-001142-puj74k7y	79	20	base	base	NN
cord-001142-puj74k7y	79	21	-	-	HYPH
cord-001142-puj74k7y	79	22	pairs	pair	NNS
cord-001142-puj74k7y	79	23	up	up	RP
cord-001142-puj74k7y	79	24	to	to	IN
cord-001142-puj74k7y	79	25	the	the	DT
cord-001142-puj74k7y	79	26	type	type	NN
cord-001142-puj74k7y	79	27	A	a	DT
cord-001142-puj74k7y	79	28	poly	poly	JJ
cord-001142-puj74k7y	79	29	-	-	HYPH
cord-001142-puj74k7y	79	30	U	u	JJ
cord-001142-puj74k7y	79	31	signal	signal	NN
cord-001142-puj74k7y	79	32	.	.	.
cord-001142-puj74k7y	80	1	Type	type	NN
cord-001142-puj74k7y	80	2	C	C	NNP
cord-001142-puj74k7y	80	3	reverse	reverse	NN
cord-001142-puj74k7y	80	4	genetics	genetics	NN
cord-001142-puj74k7y	80	5	was	be	VBD
cord-001142-puj74k7y	80	6	performed	perform	VBN
cord-001142-puj74k7y	80	7	in	in	IN
cord-001142-puj74k7y	80	8	293	293	CD
cord-001142-puj74k7y	80	9	T	T	NNP
cord-001142-puj74k7y	80	10	cells	cell	NNS
cord-001142-puj74k7y	80	11	and	and	CC
cord-001142-puj74k7y	80	12	supernatants	supernatant	NNS
cord-001142-puj74k7y	80	13	were	be	VBD
cord-001142-puj74k7y	80	14	collected	collect	VBN
cord-001142-puj74k7y	80	15	and	and	CC
cord-001142-puj74k7y	80	16	titrated	titrate	VBN
cord-001142-puj74k7y	80	17	at	at	IN
cord-001142-puj74k7y	80	18	day	day	NN
cord-001142-puj74k7y	80	19	10	10	CD
cord-001142-puj74k7y	80	20	post	post	NN
cord-001142-puj74k7y	80	21	-	-	JJ
cord-001142-puj74k7y	80	22	transfection	transfection	JJ
cord-001142-puj74k7y	80	23	(	(	-LRB-
cord-001142-puj74k7y	80	24	p.t	p.t	NNP
cord-001142-puj74k7y	80	25	.	.	NNP
cord-001142-puj74k7y	80	26	)	)	-RRB-
cord-001142-puj74k7y	80	27	.	.	.
cord-001142-puj74k7y	81	1	Type	type	VB
cord-001142-puj74k7y	81	2	A	a	DT
cord-001142-puj74k7y	81	3	reverse	reverse	JJ
cord-001142-puj74k7y	81	4	genetics	genetics	NN
cord-001142-puj74k7y	81	5	was	be	VBD
cord-001142-puj74k7y	81	6	performed	perform	VBN
cord-001142-puj74k7y	81	7	in	in	IN
cord-001142-puj74k7y	81	8	a	a	DT
cord-001142-puj74k7y	81	9	co	co	NN
cord-001142-puj74k7y	81	10	-	-	NN
cord-001142-puj74k7y	81	11	culture	culture	NN
cord-001142-puj74k7y	81	12	of	of	IN
cord-001142-puj74k7y	81	13	293	293	CD
cord-001142-puj74k7y	81	14	T	t	NN
cord-001142-puj74k7y	81	15	and	and	CC
cord-001142-puj74k7y	81	16	MDCK	MDCK	NNP
cord-001142-puj74k7y	81	17	cells	cell	NNS
cord-001142-puj74k7y	81	18	,	,	,
cord-001142-puj74k7y	81	19	and	and	CC
cord-001142-puj74k7y	81	20	supernatants	supernatant	NNS
cord-001142-puj74k7y	81	21	were	be	VBD
cord-001142-puj74k7y	81	22	collected	collect	VBN
cord-001142-puj74k7y	81	23	and	and	CC
cord-001142-puj74k7y	81	24	titrated	titrate	VBN
cord-001142-puj74k7y	81	25	at	at	IN
cord-001142-puj74k7y	81	26	72h	72h	NNP
cord-001142-puj74k7y	81	27	p.t	p.t	NNP
cord-001142-puj74k7y	81	28	.	.	.
cord-001142-puj74k7y	82	1	Titers	titer	NNS
cord-001142-puj74k7y	82	2	in	in	IN
cord-001142-puj74k7y	82	3	PFU	PFU	NNP
cord-001142-puj74k7y	83	1	/ml	/ml	NFP
cord-001142-puj74k7y	83	2	are	be	VBP
cord-001142-puj74k7y	83	3	the	the	DT
cord-001142-puj74k7y	83	4	mean	mean	NN
cord-001142-puj74k7y	83	5	of	of	IN
cord-001142-puj74k7y	83	6	2	2	CD
cord-001142-puj74k7y	83	7	to	to	IN
cord-001142-puj74k7y	83	8	4	4	CD
cord-001142-puj74k7y	83	9	independent	independent	JJ
cord-001142-puj74k7y	83	10	experiments	experiment	NNS
cord-001142-puj74k7y	83	11	.	.	.
cord-001142-puj74k7y	84	1	doi	doi	NNP
cord-001142-puj74k7y	84	2	:	:	:
cord-001142-puj74k7y	85	1	10.1371	10.1371	CD
cord-001142-puj74k7y	85	2	/	/	SYM
cord-001142-puj74k7y	85	3	journal.pone.0081550.g001	journal.pone.0081550.g001	NNS
cord-001142-puj74k7y	85	4	viruses	virus	NNS
cord-001142-puj74k7y	85	5	replicated	replicate	VBD
cord-001142-puj74k7y	85	6	to	to	IN
cord-001142-puj74k7y	85	7	similar	similar	JJ
cord-001142-puj74k7y	85	8	final	final	JJ
cord-001142-puj74k7y	85	9	titers	titer	NNS
cord-001142-puj74k7y	85	10	,	,	,
cord-001142-puj74k7y	85	11	although	although	IN
cord-001142-puj74k7y	85	12	both	both	DT
cord-001142-puj74k7y	85	13	5'A/	5'a/	CD
cord-001142-puj74k7y	85	14	3'C(C5U	3'c(c5u	CD
cord-001142-puj74k7y	85	15	)	)	-RRB-
cord-001142-puj74k7y	85	16	and	and	CC
cord-001142-puj74k7y	85	17	5'C/3'C	5'C/3'C	NNS
cord-001142-puj74k7y	85	18	-	-	:
cord-001142-puj74k7y	85	19	proxA	proxA	NNP
cord-001142-puj74k7y	85	20	were	be	VBD
cord-001142-puj74k7y	85	21	slightly	slightly	RB
cord-001142-puj74k7y	85	22	delayed	delay	VBN
cord-001142-puj74k7y	85	23	(	(	-LRB-
cord-001142-puj74k7y	85	24	Figure	figure	NN
cord-001142-puj74k7y	85	25	2B	2b	CD
cord-001142-puj74k7y	85	26	)	)	-RRB-
cord-001142-puj74k7y	85	27	.	.	.
cord-001142-puj74k7y	86	1	Overall	overall	RB
cord-001142-puj74k7y	86	2	,	,	,
cord-001142-puj74k7y	86	3	these	these	DT
cord-001142-puj74k7y	86	4	results	result	NNS
cord-001142-puj74k7y	86	5	indicated	indicate	VBD
cord-001142-puj74k7y	86	6	that	that	IN
cord-001142-puj74k7y	86	7	the	the	DT
cord-001142-puj74k7y	86	8	major	major	JJ
cord-001142-puj74k7y	86	9	packaging	packaging	NN
cord-001142-puj74k7y	86	10	signals	signal	NNS
cord-001142-puj74k7y	86	11	were	be	VBD
cord-001142-puj74k7y	86	12	not	not	RB
cord-001142-puj74k7y	86	13	located	locate	VBN
cord-001142-puj74k7y	86	14	within	within	IN
cord-001142-puj74k7y	86	15	the	the	DT
cord-001142-puj74k7y	86	16	distal	distal	JJ
cord-001142-puj74k7y	86	17	panhandle	panhandle	NN
cord-001142-puj74k7y	86	18	sequence	sequence	NN
cord-001142-puj74k7y	86	19	,	,	,
cord-001142-puj74k7y	86	20	since	since	IN
cord-001142-puj74k7y	86	21	it	-PRON-	PRP
cord-001142-puj74k7y	86	22	was	be	VBD
cord-001142-puj74k7y	86	23	possible	possible	JJ
cord-001142-puj74k7y	86	24	to	to	TO
cord-001142-puj74k7y	86	25	rescue	rescue	VB
cord-001142-puj74k7y	86	26	the	the	DT
cord-001142-puj74k7y	86	27	5'A/3'C(C5U	5'a/3'c(c5u	CD
cord-001142-puj74k7y	86	28	)	)	-RRB-
cord-001142-puj74k7y	86	29	virus	virus	NN
cord-001142-puj74k7y	86	30	.	.	.
cord-001142-puj74k7y	87	1	Thus	thus	RB
cord-001142-puj74k7y	87	2	,	,	,
cord-001142-puj74k7y	87	3	the	the	DT
cord-001142-puj74k7y	87	4	distal	distal	JJ
cord-001142-puj74k7y	87	5	sequence	sequence	NN
cord-001142-puj74k7y	87	6	of	of	IN
cord-001142-puj74k7y	87	7	the	the	DT
cord-001142-puj74k7y	87	8	type	type	NN
cord-001142-puj74k7y	87	9	C	c	NN
cord-001142-puj74k7y	87	10	3	3	CD
cord-001142-puj74k7y	87	11	'	'	''
cord-001142-puj74k7y	87	12	end	end	NN
cord-001142-puj74k7y	87	13	was	be	VBD
cord-001142-puj74k7y	87	14	not	not	RB
cord-001142-puj74k7y	87	15	restrictive	restrictive	JJ
cord-001142-puj74k7y	87	16	for	for	IN
cord-001142-puj74k7y	87	17	NS	NS	NNP
cord-001142-puj74k7y	87	18	segment	segment	NN
cord-001142-puj74k7y	87	19	packaging	packaging	NN
cord-001142-puj74k7y	87	20	.	.	.
cord-001142-puj74k7y	88	1	This	this	DT
cord-001142-puj74k7y	88	2	is	be	VBZ
cord-001142-puj74k7y	88	3	in	in	IN
cord-001142-puj74k7y	88	4	agreement	agreement	NN
cord-001142-puj74k7y	88	5	with	with	IN
cord-001142-puj74k7y	88	6	published	publish	VBN
cord-001142-puj74k7y	88	7	data	datum	NNS
cord-001142-puj74k7y	88	8	showing	show	VBG
cord-001142-puj74k7y	88	9	that	that	IN
cord-001142-puj74k7y	88	10	NS	NS	NNP
cord-001142-puj74k7y	88	11	segment	segment	NN
cord-001142-puj74k7y	88	12	packaging	packaging	NN
cord-001142-puj74k7y	88	13	does	do	VBZ
cord-001142-puj74k7y	88	14	not	not	RB
cord-001142-puj74k7y	88	15	require	require	VB
cord-001142-puj74k7y	88	16	wild	wild	JJ
cord-001142-puj74k7y	88	17	-	-	HYPH
cord-001142-puj74k7y	88	18	type	type	NN
cord-001142-puj74k7y	88	19	3	3	CD
cord-001142-puj74k7y	88	20	'	'	''
cord-001142-puj74k7y	88	21	end	end	NN
cord-001142-puj74k7y	88	22	sequences	sequence	NNS
cord-001142-puj74k7y	88	23	[	[	-LRB-
cord-001142-puj74k7y	88	24	26	26	CD
cord-001142-puj74k7y	88	25	]	]	-RRB-
cord-001142-puj74k7y	88	26	.	.	.
cord-001142-puj74k7y	89	1	The	the	DT
cord-001142-puj74k7y	89	2	putative	putative	JJ
cord-001142-puj74k7y	89	3	effect	effect	NN
cord-001142-puj74k7y	89	4	of	of	IN
cord-001142-puj74k7y	89	5	modifying	modify	VBG
cord-001142-puj74k7y	89	6	the	the	DT
cord-001142-puj74k7y	89	7	extremities	extremity	NNS
cord-001142-puj74k7y	89	8	on	on	IN
cord-001142-puj74k7y	89	9	the	the	DT
cord-001142-puj74k7y	89	10	transcription	transcription	NN
cord-001142-puj74k7y	89	11	and	and	CC
cord-001142-puj74k7y	89	12	replication	replication	NN
cord-001142-puj74k7y	89	13	steps	step	NNS
cord-001142-puj74k7y	89	14	was	be	VBD
cord-001142-puj74k7y	89	15	then	then	RB
cord-001142-puj74k7y	89	16	studied	study	VBN
cord-001142-puj74k7y	89	17	at	at	IN
cord-001142-puj74k7y	89	18	an	an	DT
cord-001142-puj74k7y	89	19	early	early	JJ
cord-001142-puj74k7y	89	20	stage	stage	NN
cord-001142-puj74k7y	89	21	of	of	IN
cord-001142-puj74k7y	89	22	the	the	DT
cord-001142-puj74k7y	89	23	viral	viral	JJ
cord-001142-puj74k7y	89	24	cycle	cycle	NN
cord-001142-puj74k7y	89	25	for	for	IN
cord-001142-puj74k7y	89	26	the	the	DT
cord-001142-puj74k7y	89	27	three	three	CD
cord-001142-puj74k7y	89	28	rescued	rescue	VBN
cord-001142-puj74k7y	89	29	type	type	NN
cord-001142-puj74k7y	89	30	A	a	NN
cord-001142-puj74k7y	89	31	viruses	virus	NNS
cord-001142-puj74k7y	89	32	.	.	.
cord-001142-puj74k7y	90	1	Levels	level	NNS
cord-001142-puj74k7y	90	2	of	of	IN
cord-001142-puj74k7y	90	3	mRNA	mrna	NN
cord-001142-puj74k7y	90	4	and	and	CC
cord-001142-puj74k7y	90	5	vRNA	vRNA	NNPS
cord-001142-puj74k7y	90	6	were	be	VBD
cord-001142-puj74k7y	90	7	analyzed	analyze	VBN
cord-001142-puj74k7y	90	8	by	by	IN
cord-001142-puj74k7y	90	9	RT	RT	NNP
cord-001142-puj74k7y	90	10	-	-	HYPH
cord-001142-puj74k7y	90	11	qPCR	qPCR	NNP
cord-001142-puj74k7y	90	12	[	[	-LRB-
cord-001142-puj74k7y	90	13	23	23	CD
cord-001142-puj74k7y	90	14	]	]	-RRB-
cord-001142-puj74k7y	90	15	at	at	IN
cord-001142-puj74k7y	90	16	6	6	CD
cord-001142-puj74k7y	90	17	hours	hour	NNS
cord-001142-puj74k7y	90	18	post	post	NN
cord-001142-puj74k7y	90	19	infection	infection	NN
cord-001142-puj74k7y	90	20	(	(	-LRB-
cord-001142-puj74k7y	90	21	h.p.i	h.p.i	NNP
cord-001142-puj74k7y	90	22	.	.	NNP
cord-001142-puj74k7y	90	23	)	)	-RRB-
cord-001142-puj74k7y	90	24	at	at	IN
cord-001142-puj74k7y	90	25	high	high	JJ
cord-001142-puj74k7y	90	26	m.o.i	m.o.i	NN
cord-001142-puj74k7y	90	27	.	.	NNP
cord-001142-puj74k7y	90	28	,	,	,
cord-001142-puj74k7y	90	29	using	use	VBG
cord-001142-puj74k7y	90	30	RT	RT	NNP
cord-001142-puj74k7y	90	31	primers	primer	NNS
cord-001142-puj74k7y	90	32	specific	specific	JJ
cord-001142-puj74k7y	90	33	for	for	IN
cord-001142-puj74k7y	90	34	mRNA	mRNA	NNP
cord-001142-puj74k7y	90	35	or	or	CC
cord-001142-puj74k7y	90	36	vRNA	vrna	JJ
cord-001142-puj74k7y	90	37	(	(	-LRB-
cord-001142-puj74k7y	90	38	Figure	figure	NN
cord-001142-puj74k7y	90	39	3A	3a	CD
cord-001142-puj74k7y	90	40	)	)	-RRB-
cord-001142-puj74k7y	90	41	.	.	.
cord-001142-puj74k7y	91	1	Crossing	crossing	NN
cord-001142-puj74k7y	91	2	point	point	NN
cord-001142-puj74k7y	91	3	values	value	NNS
cord-001142-puj74k7y	91	4	were	be	VBD
cord-001142-puj74k7y	91	5	used	use	VBN
cord-001142-puj74k7y	91	6	to	to	TO
cord-001142-puj74k7y	91	7	evaluate	evaluate	VB
cord-001142-puj74k7y	91	8	the	the	DT
cord-001142-puj74k7y	91	9	relative	relative	JJ
cord-001142-puj74k7y	91	10	level	level	NN
cord-001142-puj74k7y	91	11	of	of	IN
cord-001142-puj74k7y	91	12	both	both	DT
cord-001142-puj74k7y	91	13	processes	process	NNS
cord-001142-puj74k7y	91	14	,	,	,
cord-001142-puj74k7y	91	15	by	by	IN
cord-001142-puj74k7y	91	16	calculating	calculate	VBG
cord-001142-puj74k7y	91	17	an	an	DT
cord-001142-puj74k7y	91	18	mRNA	mrna	NN
cord-001142-puj74k7y	91	19	/	/	SYM
cord-001142-puj74k7y	91	20	vRNA	vrna	JJ
cord-001142-puj74k7y	91	21	ratio	ratio	NN
cord-001142-puj74k7y	91	22	.	.	.
cord-001142-puj74k7y	92	1	Compared	compare	VBN
cord-001142-puj74k7y	92	2	to	to	IN
cord-001142-puj74k7y	92	3	wild	wild	JJ
cord-001142-puj74k7y	92	4	-	-	HYPH
cord-001142-puj74k7y	92	5	type	type	NN
cord-001142-puj74k7y	92	6	5'A/	5'a/	CD
cord-001142-puj74k7y	92	7	3'A	3'a	NN
cord-001142-puj74k7y	92	8	,	,	,
cord-001142-puj74k7y	92	9	significant	significant	JJ
cord-001142-puj74k7y	92	10	differences	difference	NNS
cord-001142-puj74k7y	92	11	(	(	-LRB-
cord-001142-puj74k7y	92	12	p<0.05	p<0.05	CD
cord-001142-puj74k7y	92	13	)	)	-RRB-
cord-001142-puj74k7y	92	14	were	be	VBD
cord-001142-puj74k7y	92	15	found	find	VBN
cord-001142-puj74k7y	92	16	only	only	RB
cord-001142-puj74k7y	92	17	for	for	IN
cord-001142-puj74k7y	92	18	5'A/	5'a/	CD
cord-001142-puj74k7y	92	19	3'C(C5U	3'c(c5u	CD
cord-001142-puj74k7y	92	20	)	)	-RRB-
cord-001142-puj74k7y	92	21	,	,	,
cord-001142-puj74k7y	92	22	but	but	CC
cord-001142-puj74k7y	92	23	not	not	RB
cord-001142-puj74k7y	92	24	for	for	IN
cord-001142-puj74k7y	92	25	5'C/3'C	5'c/3'c	NN
cord-001142-puj74k7y	92	26	-	-	:
cord-001142-puj74k7y	92	27	proxA.	proxa.	JJ
cord-001142-puj74k7y	93	1	Interestingly	interestingly	RB
cord-001142-puj74k7y	93	2	,	,	,
cord-001142-puj74k7y	93	3	the	the	DT
cord-001142-puj74k7y	93	4	CpmRNA	CpmRNA	NNPS
cord-001142-puj74k7y	93	5	/	/	SYM
cord-001142-puj74k7y	93	6	CpvRNA	CpvRNA	NNP
cord-001142-puj74k7y	93	7	ratio	ratio	NN
cord-001142-puj74k7y	93	8	for	for	IN
cord-001142-puj74k7y	93	9	5'A/3'C(C5U	5'a/3'c(c5u	CD
cord-001142-puj74k7y	93	10	)	)	-RRB-
cord-001142-puj74k7y	93	11	was	be	VBD
cord-001142-puj74k7y	93	12	higher	high	JJR
cord-001142-puj74k7y	93	13	than	than	IN
cord-001142-puj74k7y	93	14	for	for	IN
cord-001142-puj74k7y	93	15	wild	wild	JJ
cord-001142-puj74k7y	93	16	-	-	HYPH
cord-001142-puj74k7y	93	17	type	type	NN
cord-001142-puj74k7y	93	18	5'A/3'A	5'a/3'a	NN
cord-001142-puj74k7y	93	19	for	for	IN
cord-001142-puj74k7y	93	20	all	all	DT
cord-001142-puj74k7y	93	21	segments	segment	NNS
cord-001142-puj74k7y	93	22	,	,	,
cord-001142-puj74k7y	93	23	meaning	mean	VBG
cord-001142-puj74k7y	93	24	that	that	IN
cord-001142-puj74k7y	93	25	either	either	CC
cord-001142-puj74k7y	93	26	more	more	RBR
cord-001142-puj74k7y	93	27	vRNA	vRNA	JJS
cord-001142-puj74k7y	93	28	or	or	CC
cord-001142-puj74k7y	93	29	less	less	JJR
cord-001142-puj74k7y	93	30	mRNA	mRNA	NNP
cord-001142-puj74k7y	93	31	was	be	VBD
cord-001142-puj74k7y	93	32	synthesized	synthesize	VBN
cord-001142-puj74k7y	93	33	for	for	IN
cord-001142-puj74k7y	93	34	5'A/3'C(C5U	5'a/3'c(c5u	CD
cord-001142-puj74k7y	93	35	)	)	-RRB-
cord-001142-puj74k7y	93	36	.	.	.
cord-001142-puj74k7y	94	1	The	the	DT
cord-001142-puj74k7y	94	2	sole	sole	JJ
cord-001142-puj74k7y	94	3	difference	difference	NN
cord-001142-puj74k7y	94	4	between	between	IN
cord-001142-puj74k7y	94	5	5'A/3'C(C5U	5'a/3'c(c5u	CD
cord-001142-puj74k7y	94	6	)	)	-RRB-
cord-001142-puj74k7y	94	7	and	and	CC
cord-001142-puj74k7y	94	8	wild	wild	JJ
cord-001142-puj74k7y	94	9	-	-	HYPH
cord-001142-puj74k7y	94	10	type	type	NN
cord-001142-puj74k7y	94	11	5'A/3'A	5'a/3'a	NN
cord-001142-puj74k7y	94	12	viruses	virus	NNS
cord-001142-puj74k7y	94	13	being	be	VBG
cord-001142-puj74k7y	94	14	the	the	DT
cord-001142-puj74k7y	94	15	3	3	CD
cord-001142-puj74k7y	94	16	'	'	POS
cord-001142-puj74k7y	94	17	distal	distal	JJ
cord-001142-puj74k7y	94	18	extremity	extremity	NN
cord-001142-puj74k7y	94	19	of	of	IN
cord-001142-puj74k7y	94	20	the	the	DT
cord-001142-puj74k7y	94	21	NS	NS	NNP
cord-001142-puj74k7y	94	22	segment	segment	NN
cord-001142-puj74k7y	94	23	suggested	suggest	VBD
cord-001142-puj74k7y	94	24	that	that	IN
cord-001142-puj74k7y	94	25	the	the	DT
cord-001142-puj74k7y	94	26	level	level	NN
cord-001142-puj74k7y	94	27	of	of	IN
cord-001142-puj74k7y	94	28	NS	NS	NNP
cord-001142-puj74k7y	94	29	encoded	encode	VBD
cord-001142-puj74k7y	94	30	proteins	protein	NNS
cord-001142-puj74k7y	94	31	(	(	-LRB-
cord-001142-puj74k7y	94	32	i.e.	i.e.	FW
cord-001142-puj74k7y	94	33	NS1	ns1	NN
cord-001142-puj74k7y	94	34	or	or	CC
cord-001142-puj74k7y	94	35	/	/	SYM
cord-001142-puj74k7y	94	36	and	and	CC
cord-001142-puj74k7y	94	37	NS2	NS2	NNP
cord-001142-puj74k7y	94	38	/	/	SYM
cord-001142-puj74k7y	94	39	NEP	NEP	NNP
cord-001142-puj74k7y	94	40	)	)	-RRB-
cord-001142-puj74k7y	94	41	was	be	VBD
cord-001142-puj74k7y	94	42	affected	affect	VBN
cord-001142-puj74k7y	94	43	at	at	IN
cord-001142-puj74k7y	94	44	early	early	JJ
cord-001142-puj74k7y	94	45	stages	stage	NNS
cord-001142-puj74k7y	94	46	of	of	IN
cord-001142-puj74k7y	94	47	infection	infection	NN
cord-001142-puj74k7y	94	48	for	for	IN
cord-001142-puj74k7y	94	49	this	this	DT
cord-001142-puj74k7y	94	50	virus	virus	NN
cord-001142-puj74k7y	94	51	(	(	-LRB-
cord-001142-puj74k7y	94	52	5'A/3'C(C5U	5'a/3'c(c5u	CD
cord-001142-puj74k7y	94	53	)	)	-RRB-
cord-001142-puj74k7y	94	54	)	)	-RRB-
cord-001142-puj74k7y	94	55	.	.	.
cord-001142-puj74k7y	95	1	Western	western	JJ
cord-001142-puj74k7y	95	2	-	-	HYPH
cord-001142-puj74k7y	95	3	blot	blot	NN
cord-001142-puj74k7y	95	4	was	be	VBD
cord-001142-puj74k7y	95	5	used	use	VBN
cord-001142-puj74k7y	95	6	to	to	TO
cord-001142-puj74k7y	95	7	evaluate	evaluate	VB
cord-001142-puj74k7y	95	8	the	the	DT
cord-001142-puj74k7y	95	9	amount	amount	NN
cord-001142-puj74k7y	95	10	of	of	IN
cord-001142-puj74k7y	95	11	NS	NS	NNP
cord-001142-puj74k7y	95	12	encoded	encode	VBD
cord-001142-puj74k7y	95	13	proteins	protein	NNS
cord-001142-puj74k7y	95	14	two	two	CD
cord-001142-puj74k7y	95	15	hours	hour	NNS
cord-001142-puj74k7y	95	16	before	before	IN
cord-001142-puj74k7y	95	17	the	the	DT
cord-001142-puj74k7y	95	18	observed	observed	JJ
cord-001142-puj74k7y	95	19	effect	effect	NN
cord-001142-puj74k7y	95	20	.	.	.
cord-001142-puj74k7y	96	1	The	the	DT
cord-001142-puj74k7y	96	2	amount	amount	NN
cord-001142-puj74k7y	96	3	of	of	IN
cord-001142-puj74k7y	96	4	NP	NP	NNP
cord-001142-puj74k7y	96	5	protein	protein	NN
cord-001142-puj74k7y	96	6	was	be	VBD
cord-001142-puj74k7y	96	7	used	use	VBN
cord-001142-puj74k7y	96	8	to	to	TO
cord-001142-puj74k7y	96	9	normalize	normalize	VB
cord-001142-puj74k7y	96	10	the	the	DT
cord-001142-puj74k7y	96	11	results	result	NNS
cord-001142-puj74k7y	96	12	.	.	.
cord-001142-puj74k7y	97	1	At	at	IN
cord-001142-puj74k7y	97	2	4	4	CD
cord-001142-puj74k7y	97	3	h.p.i	h.p.i	NNP
cord-001142-puj74k7y	97	4	.	.	.
cord-001142-puj74k7y	97	5	at	at	IN
cord-001142-puj74k7y	97	6	high	high	JJ
cord-001142-puj74k7y	97	7	m.o.i	m.o.i	NN
cord-001142-puj74k7y	97	8	.	.	NNP
cord-001142-puj74k7y	97	9	,	,	,
cord-001142-puj74k7y	97	10	only	only	RB
cord-001142-puj74k7y	97	11	5'A/	5'a/	CD
cord-001142-puj74k7y	97	12	3'C(C5U	3'c(c5u	CD
cord-001142-puj74k7y	97	13	)	)	-RRB-
cord-001142-puj74k7y	97	14	showed	show	VBD
cord-001142-puj74k7y	97	15	a	a	DT
cord-001142-puj74k7y	97	16	clear	clear	JJ
cord-001142-puj74k7y	97	17	increase	increase	NN
cord-001142-puj74k7y	97	18	in	in	IN
cord-001142-puj74k7y	97	19	the	the	DT
cord-001142-puj74k7y	97	20	production	production	NN
cord-001142-puj74k7y	97	21	of	of	IN
cord-001142-puj74k7y	97	22	NS1	ns1	NN
cord-001142-puj74k7y	97	23	protein	protein	NN
cord-001142-puj74k7y	97	24	,	,	,
cord-001142-puj74k7y	97	25	as	as	IN
cord-001142-puj74k7y	97	26	indicated	indicate	VBN
cord-001142-puj74k7y	97	27	by	by	IN
cord-001142-puj74k7y	97	28	a	a	DT
cord-001142-puj74k7y	97	29	higher	high	JJR
cord-001142-puj74k7y	97	30	NS1	NS1	NNS
cord-001142-puj74k7y	97	31	/	/	SYM
cord-001142-puj74k7y	97	32	NP	np	NN
cord-001142-puj74k7y	97	33	ratio	ratio	NN
cord-001142-puj74k7y	97	34	(	(	-LRB-
cord-001142-puj74k7y	97	35	Figure	figure	NN
cord-001142-puj74k7y	97	36	3B	3b	CD
cord-001142-puj74k7y	97	37	)	)	-RRB-
cord-001142-puj74k7y	97	38	.	.	.
cord-001142-puj74k7y	98	1	The	the	DT
cord-001142-puj74k7y	98	2	putative	putative	JJ
cord-001142-puj74k7y	98	3	roles	role	NNS
cord-001142-puj74k7y	98	4	of	of	IN
cord-001142-puj74k7y	98	5	the	the	DT
cord-001142-puj74k7y	98	6	proteins	protein	NNS
cord-001142-puj74k7y	98	7	encoded	encode	VBN
cord-001142-puj74k7y	98	8	by	by	IN
cord-001142-puj74k7y	98	9	the	the	DT
cord-001142-puj74k7y	98	10	NS	NS	NNP
cord-001142-puj74k7y	98	11	segment	segment	NN
cord-001142-puj74k7y	98	12	in	in	IN
cord-001142-puj74k7y	98	13	viral	viral	JJ
cord-001142-puj74k7y	98	14	transcription	transcription	NN
cord-001142-puj74k7y	98	15	and	and	CC
cord-001142-puj74k7y	98	16	replication	replication	NN
cord-001142-puj74k7y	98	17	have	have	VBP
cord-001142-puj74k7y	98	18	been	be	VBN
cord-001142-puj74k7y	98	19	documented	document	VBN
cord-001142-puj74k7y	99	1	:	:	:
cord-001142-puj74k7y	99	2	(	(	-LRB-
cord-001142-puj74k7y	99	3	i	i	NN
cord-001142-puj74k7y	99	4	)	)	-RRB-
cord-001142-puj74k7y	99	5	inactivation	inactivation	NN
cord-001142-puj74k7y	99	6	of	of	IN
cord-001142-puj74k7y	99	7	NS1	ns1	NN
cord-001142-puj74k7y	99	8	protein	protein	NN
cord-001142-puj74k7y	99	9	reduces	reduce	VBZ
cord-001142-puj74k7y	99	10	the	the	DT
cord-001142-puj74k7y	99	11	amount	amount	NN
cord-001142-puj74k7y	99	12	of	of	IN
cord-001142-puj74k7y	99	13	all	all	DT
cord-001142-puj74k7y	99	14	vRNAs	vrna	NNS
cord-001142-puj74k7y	99	15	without	without	IN
cord-001142-puj74k7y	99	16	affecting	affect	VBG
cord-001142-puj74k7y	99	17	the	the	DT
cord-001142-puj74k7y	99	18	amount	amount	NN
cord-001142-puj74k7y	99	19	of	of	IN
cord-001142-puj74k7y	99	20	mRNAs	mrnas	CD
cord-001142-puj74k7y	99	21	[	[	-LRB-
cord-001142-puj74k7y	99	22	27	27	CD
cord-001142-puj74k7y	99	23	]	]	-RRB-
cord-001142-puj74k7y	99	24	;	;	:
cord-001142-puj74k7y	99	25	(	(	-LRB-
cord-001142-puj74k7y	99	26	ii	ii	NN
cord-001142-puj74k7y	99	27	)	)	-RRB-
cord-001142-puj74k7y	99	28	NS1	ns1	NN
cord-001142-puj74k7y	99	29	protein	protein	NN
cord-001142-puj74k7y	99	30	counteracts	counteract	VBZ
cord-001142-puj74k7y	99	31	the	the	DT
cord-001142-puj74k7y	99	32	effect	effect	NN
cord-001142-puj74k7y	99	33	of	of	IN
cord-001142-puj74k7y	99	34	a	a	DT
cord-001142-puj74k7y	99	35	polymerase	polymerase	NN
cord-001142-puj74k7y	99	36	regulating	regulating	NN
cord-001142-puj74k7y	99	37	factor	factor	NN
cord-001142-puj74k7y	99	38	,	,	,
cord-001142-puj74k7y	99	39	possibly	possibly	RB
cord-001142-puj74k7y	99	40	hnRNP	hnrnp	NN
cord-001142-puj74k7y	99	41	-	-	HYPH
cord-001142-puj74k7y	99	42	F	F	NNP
cord-001142-puj74k7y	99	43	[	[	-LRB-
cord-001142-puj74k7y	99	44	28	28	CD
cord-001142-puj74k7y	99	45	]	]	-RRB-
cord-001142-puj74k7y	99	46	,	,	,
cord-001142-puj74k7y	99	47	that	that	WDT
cord-001142-puj74k7y	99	48	inhibits	inhibit	VBZ
cord-001142-puj74k7y	99	49	influenza	influenza	NN
cord-001142-puj74k7y	99	50	virus	virus	NN
cord-001142-puj74k7y	99	51	full	full	JJ
cord-001142-puj74k7y	99	52	-	-	HYPH
cord-001142-puj74k7y	99	53	length	length	NN
cord-001142-puj74k7y	99	54	RNA	RNA	NNP
cord-001142-puj74k7y	99	55	synthesis	synthesis	NN
cord-001142-puj74k7y	99	56	[	[	-LRB-
cord-001142-puj74k7y	99	57	29	29	CD
cord-001142-puj74k7y	99	58	]	]	-RRB-
cord-001142-puj74k7y	99	59	;	;	:
cord-001142-puj74k7y	99	60	and	and	CC
cord-001142-puj74k7y	99	61	(	(	-LRB-
cord-001142-puj74k7y	99	62	iii	iii	NN
cord-001142-puj74k7y	99	63	)	)	-RRB-
cord-001142-puj74k7y	99	64	NS2	NS2	NNP
cord-001142-puj74k7y	99	65	/	/	SYM
cord-001142-puj74k7y	99	66	NEP	NEP	NNP
cord-001142-puj74k7y	99	67	protein	protein	NN
cord-001142-puj74k7y	99	68	reduces	reduce	VBZ
cord-001142-puj74k7y	99	69	transcription	transcription	NN
cord-001142-puj74k7y	99	70	and	and	CC
cord-001142-puj74k7y	99	71	increases	increase	VBZ
cord-001142-puj74k7y	99	72	replication	replication	NN
cord-001142-puj74k7y	99	73	[	[	-LRB-
cord-001142-puj74k7y	99	74	30	30	CD
cord-001142-puj74k7y	99	75	]	]	-RRB-
cord-001142-puj74k7y	99	76	.	.	.
cord-001142-puj74k7y	100	1	The	the	DT
cord-001142-puj74k7y	100	2	latter	latter	JJ
cord-001142-puj74k7y	100	3	role	role	NN
cord-001142-puj74k7y	100	4	of	of	IN
cord-001142-puj74k7y	100	5	NS2	NS2	NNP
cord-001142-puj74k7y	100	6	/	/	SYM
cord-001142-puj74k7y	100	7	NEP	NEP	NNP
cord-001142-puj74k7y	100	8	could	could	MD
cord-001142-puj74k7y	100	9	be	be	VB
cord-001142-puj74k7y	100	10	indirect	indirect	JJ
cord-001142-puj74k7y	100	11	by	by	IN
cord-001142-puj74k7y	100	12	stimulating	stimulate	VBG
cord-001142-puj74k7y	100	13	the	the	DT
cord-001142-puj74k7y	100	14	synthesis	synthesis	NN
cord-001142-puj74k7y	100	15	of	of	IN
cord-001142-puj74k7y	100	16	virus	virus	NN
cord-001142-puj74k7y	100	17	-	-	HYPH
cord-001142-puj74k7y	100	18	derived	derive	VBN
cord-001142-puj74k7y	100	19	small	small	JJ
cord-001142-puj74k7y	100	20	viral	viral	JJ
cord-001142-puj74k7y	100	21	RNAs	rna	NNS
cord-001142-puj74k7y	100	22	,	,	,
cord-001142-puj74k7y	100	23	whose	whose	WP$
cord-001142-puj74k7y	100	24	depletion	depletion	NN
cord-001142-puj74k7y	100	25	was	be	VBD
cord-001142-puj74k7y	100	26	shown	show	VBN
cord-001142-puj74k7y	100	27	to	to	TO
cord-001142-puj74k7y	100	28	reduce	reduce	VB
cord-001142-puj74k7y	100	29	vRNA	vrna	JJ
cord-001142-puj74k7y	100	30	synthesis	synthesis	NN
cord-001142-puj74k7y	100	31	[	[	-LRB-
cord-001142-puj74k7y	100	32	31	31	CD
cord-001142-puj74k7y	100	33	]	]	-RRB-
cord-001142-puj74k7y	100	34	.	.	.
cord-001142-puj74k7y	101	1	Thus	thus	RB
cord-001142-puj74k7y	101	2	,	,	,
cord-001142-puj74k7y	101	3	the	the	DT
cord-001142-puj74k7y	101	4	decreased	decrease	VBN
cord-001142-puj74k7y	101	5	mRNA/	mRNA/	NNP
cord-001142-puj74k7y	101	6	vRNA	vRNA	NNP
cord-001142-puj74k7y	101	7	ratio	ratio	NN
cord-001142-puj74k7y	101	8	observed	observe	VBN
cord-001142-puj74k7y	101	9	at	at	IN
cord-001142-puj74k7y	101	10	6	6	CD
cord-001142-puj74k7y	101	11	h.p.i	h.p.i	NNP
cord-001142-puj74k7y	101	12	.	.	.
cord-001142-puj74k7y	101	13	at	at	IN
cord-001142-puj74k7y	101	14	high	high	JJ
cord-001142-puj74k7y	101	15	m.o.i	m.o.i	NN
cord-001142-puj74k7y	101	16	.	.	.
cord-001142-puj74k7y	101	17	could	could	MD
cord-001142-puj74k7y	101	18	reflect	reflect	VB
cord-001142-puj74k7y	101	19	an	an	DT
cord-001142-puj74k7y	101	20	increase	increase	NN
cord-001142-puj74k7y	101	21	in	in	IN
cord-001142-puj74k7y	101	22	replication	replication	NN
cord-001142-puj74k7y	101	23	,	,	,
cord-001142-puj74k7y	101	24	which	which	WDT
cord-001142-puj74k7y	101	25	would	would	MD
cord-001142-puj74k7y	101	26	be	be	VB
cord-001142-puj74k7y	101	27	consistent	consistent	JJ
cord-001142-puj74k7y	101	28	with	with	IN
cord-001142-puj74k7y	101	29	the	the	DT
cord-001142-puj74k7y	101	30	higher	high	JJR
cord-001142-puj74k7y	101	31	viral	viral	JJ
cord-001142-puj74k7y	101	32	titer	titer	NN
cord-001142-puj74k7y	101	33	for	for	IN
cord-001142-puj74k7y	101	34	5'A/3'C(C5U	5'a/3'c(c5u	CD
cord-001142-puj74k7y	101	35	)	)	-RRB-
cord-001142-puj74k7y	102	1	we	-PRON-	PRP
cord-001142-puj74k7y	102	2	observed	observe	VBD
cord-001142-puj74k7y	102	3	at	at	IN
cord-001142-puj74k7y	102	4	that	that	DT
cord-001142-puj74k7y	102	5	time	time	NN
cord-001142-puj74k7y	102	6	point	point	NN
cord-001142-puj74k7y	102	7	(	(	-LRB-
cord-001142-puj74k7y	102	8	5x10	5x10	CD
cord-001142-puj74k7y	102	9	5	5	CD
cord-001142-puj74k7y	102	10	vs	vs	IN
cord-001142-puj74k7y	102	11	1.6x10	1.6x10	CD
cord-001142-puj74k7y	102	12	7	7	CD
cord-001142-puj74k7y	102	13	PFU	pfu	NN
cord-001142-puj74k7y	102	14	/	/	SYM
cord-001142-puj74k7y	102	15	ml	ml	NN
cord-001142-puj74k7y	102	16	for	for	IN
cord-001142-puj74k7y	102	17	wild	wild	JJ
cord-001142-puj74k7y	102	18	-	-	HYPH
cord-001142-puj74k7y	102	19	type	type	NN
cord-001142-puj74k7y	102	20	and	and	CC
cord-001142-puj74k7y	102	21	5'A/3'C(C5U	5'a/3'c(c5u	CD
cord-001142-puj74k7y	102	22	)	)	-RRB-
cord-001142-puj74k7y	102	23	,	,	,
cord-001142-puj74k7y	102	24	respectively	respectively	RB
cord-001142-puj74k7y	102	25	)	)	-RRB-
cord-001142-puj74k7y	102	26	.	.	.
cord-001142-puj74k7y	103	1	The	the	DT
cord-001142-puj74k7y	103	2	most	most	RBS
cord-001142-puj74k7y	103	3	likely	likely	JJ
cord-001142-puj74k7y	103	4	hypothesis	hypothesis	NN
cord-001142-puj74k7y	103	5	for	for	IN
cord-001142-puj74k7y	103	6	the	the	DT
cord-001142-puj74k7y	103	7	increased	increase	VBN
cord-001142-puj74k7y	103	8	amount	amount	NN
cord-001142-puj74k7y	103	9	of	of	IN
cord-001142-puj74k7y	103	10	5'A/	5'a/	CD
cord-001142-puj74k7y	103	11	3'C(C5U	3'c(c5u	CD
cord-001142-puj74k7y	103	12	)	)	-RRB-
cord-001142-puj74k7y	104	1	NS	NS	NNP
cord-001142-puj74k7y	104	2	proteins	protein	NNS
cord-001142-puj74k7y	104	3	is	be	VBZ
cord-001142-puj74k7y	104	4	that	that	IN
cord-001142-puj74k7y	104	5	the	the	DT
cord-001142-puj74k7y	104	6	level	level	NN
cord-001142-puj74k7y	104	7	of	of	IN
cord-001142-puj74k7y	104	8	transcription	transcription	NN
cord-001142-puj74k7y	104	9	was	be	VBD
cord-001142-puj74k7y	104	10	affected	affect	VBN
cord-001142-puj74k7y	104	11	and	and	CC
cord-001142-puj74k7y	104	12	that	that	IN
cord-001142-puj74k7y	104	13	more	more	JJR
cord-001142-puj74k7y	104	14	mRNA	mRNA	NNP
cord-001142-puj74k7y	104	15	was	be	VBD
cord-001142-puj74k7y	104	16	synthesized	synthesize	VBN
cord-001142-puj74k7y	104	17	for	for	IN
cord-001142-puj74k7y	104	18	5'A/	5'a/	CD
cord-001142-puj74k7y	104	19	3'C(C5U	3'c(c5u	CD
cord-001142-puj74k7y	104	20	)	)	-RRB-
cord-001142-puj74k7y	104	21	at	at	IN
cord-001142-puj74k7y	104	22	earlier	early	JJR
cord-001142-puj74k7y	104	23	stages	stage	NNS
cord-001142-puj74k7y	104	24	of	of	IN
cord-001142-puj74k7y	104	25	infection	infection	NN
cord-001142-puj74k7y	104	26	(	(	-LRB-
cord-001142-puj74k7y	104	27	2	2	CD
cord-001142-puj74k7y	104	28	-	-	SYM
cord-001142-puj74k7y	104	29	4	4	CD
cord-001142-puj74k7y	104	30	h.p.i	h.p.i	NNP
cord-001142-puj74k7y	104	31	.	.	NNP
cord-001142-puj74k7y	104	32	)	)	-RRB-
cord-001142-puj74k7y	104	33	.	.	.
cord-001142-puj74k7y	105	1	Such	such	PDT
cord-001142-puj74k7y	105	2	a	a	DT
cord-001142-puj74k7y	105	3	trend	trend	NN
cord-001142-puj74k7y	105	4	,	,	,
cord-001142-puj74k7y	105	5	but	but	CC
cord-001142-puj74k7y	105	6	no	no	DT
cord-001142-puj74k7y	105	7	significant	significant	JJ
cord-001142-puj74k7y	105	8	difference	difference	NN
cord-001142-puj74k7y	105	9	,	,	,
cord-001142-puj74k7y	105	10	in	in	IN
cord-001142-puj74k7y	105	11	NS	NS	NNP
cord-001142-puj74k7y	106	1	mRNA	mrna	NN
cord-001142-puj74k7y	106	2	quantification	quantification	NN
cord-001142-puj74k7y	106	3	after	after	IN
cord-001142-puj74k7y	106	4	normalization	normalization	NN
cord-001142-puj74k7y	106	5	relative	relative	JJ
cord-001142-puj74k7y	106	6	to	to	IN
cord-001142-puj74k7y	106	7	GAPDH	GAPDH	NNP
cord-001142-puj74k7y	106	8	was	be	VBD
cord-001142-puj74k7y	106	9	indeed	indeed	RB
cord-001142-puj74k7y	106	10	observed	observe	VBN
cord-001142-puj74k7y	106	11	between	between	IN
cord-001142-puj74k7y	106	12	two	two	CD
cord-001142-puj74k7y	106	13	and	and	CC
cord-001142-puj74k7y	106	14	four	four	CD
cord-001142-puj74k7y	106	15	h.p.i	h.p.i	NNP
cord-001142-puj74k7y	106	16	.	.	.
cord-001142-puj74k7y	106	17	(	(	-LRB-
cord-001142-puj74k7y	106	18	data	datum	NNS
cord-001142-puj74k7y	106	19	not	not	RB
cord-001142-puj74k7y	106	20	shown	show	VBN
cord-001142-puj74k7y	106	21	)	)	-RRB-
cord-001142-puj74k7y	106	22	.	.	.
cord-001142-puj74k7y	107	1	The	the	DT
cord-001142-puj74k7y	107	2	difference	difference	NN
cord-001142-puj74k7y	107	3	in	in	IN
cord-001142-puj74k7y	107	4	the	the	DT
cord-001142-puj74k7y	107	5	base	base	NN
cord-001142-puj74k7y	107	6	-	-	HYPH
cord-001142-puj74k7y	107	7	pairing	pair	VBG
cord-001142-puj74k7y	107	8	strength	strength	NN
cord-001142-puj74k7y	107	9	of	of	IN
cord-001142-puj74k7y	107	10	the	the	DT
cord-001142-puj74k7y	107	11	distal	distal	JJ
cord-001142-puj74k7y	107	12	panhandle	panhandle	NN
cord-001142-puj74k7y	107	13	formed	form	VBN
cord-001142-puj74k7y	107	14	by	by	IN
cord-001142-puj74k7y	107	15	the	the	DT
cord-001142-puj74k7y	107	16	heterotypic	heterotypic	NNP
cord-001142-puj74k7y	107	17	extremities	extremity	NNS
cord-001142-puj74k7y	107	18	in	in	IN
cord-001142-puj74k7y	107	19	5'A/3'C(C5U	5'a/3'c(c5u	CD
cord-001142-puj74k7y	107	20	)	)	-RRB-
cord-001142-puj74k7y	107	21	compared	compare	VBN
cord-001142-puj74k7y	107	22	to	to	IN
cord-001142-puj74k7y	107	23	wild	wild	JJ
cord-001142-puj74k7y	107	24	-	-	HYPH
cord-001142-puj74k7y	107	25	type	type	NN
cord-001142-puj74k7y	107	26	,	,	,
cord-001142-puj74k7y	107	27	evaluated	evaluate	VBN
cord-001142-puj74k7y	107	28	by	by	IN
cord-001142-puj74k7y	107	29	scores	score	NNS
cord-001142-puj74k7y	107	30	calculated	calculate	VBN
cord-001142-puj74k7y	107	31	based	base	VBN
cord-001142-puj74k7y	107	32	on	on	IN
cord-001142-puj74k7y	107	33	the	the	DT
cord-001142-puj74k7y	107	34	number	number	NN
cord-001142-puj74k7y	107	35	and	and	CC
cord-001142-puj74k7y	107	36	nature	nature	NN
cord-001142-puj74k7y	107	37	of	of	IN
cord-001142-puj74k7y	107	38	the	the	DT
cord-001142-puj74k7y	107	39	base	base	NN
cord-001142-puj74k7y	107	40	-	-	HYPH
cord-001142-puj74k7y	107	41	pairs	pair	NNS
cord-001142-puj74k7y	107	42	(	(	-LRB-
cord-001142-puj74k7y	107	43	Figure	figure	NN
cord-001142-puj74k7y	107	44	1	1	CD
cord-001142-puj74k7y	107	45	)	)	-RRB-
cord-001142-puj74k7y	107	46	,	,	,
cord-001142-puj74k7y	107	47	could	could	MD
cord-001142-puj74k7y	107	48	favour	favour	VB
cord-001142-puj74k7y	107	49	the	the	DT
cord-001142-puj74k7y	107	50	attachment	attachment	NN
cord-001142-puj74k7y	107	51	of	of	IN
cord-001142-puj74k7y	107	52	the	the	DT
cord-001142-puj74k7y	107	53	polymerase	polymerase	NN
cord-001142-puj74k7y	107	54	.	.	.
cord-001142-puj74k7y	108	1	The	the	DT
cord-001142-puj74k7y	108	2	stability	stability	NN
cord-001142-puj74k7y	108	3	of	of	IN
cord-001142-puj74k7y	108	4	the	the	DT
cord-001142-puj74k7y	108	5	duplex	duplex	NN
cord-001142-puj74k7y	108	6	formed	form	VBN
cord-001142-puj74k7y	108	7	by	by	IN
cord-001142-puj74k7y	108	8	the	the	DT
cord-001142-puj74k7y	108	9	5	5	CD
cord-001142-puj74k7y	108	10	'	'	''
cord-001142-puj74k7y	108	11	and	and	CC
cord-001142-puj74k7y	108	12	3	3	CD
cord-001142-puj74k7y	108	13	'	'	''
cord-001142-puj74k7y	108	14	ends	end	NNS
cord-001142-puj74k7y	108	15	was	be	VBD
cord-001142-puj74k7y	108	16	indeed	indeed	RB
cord-001142-puj74k7y	108	17	shown	show	VBN
cord-001142-puj74k7y	108	18	to	to	TO
cord-001142-puj74k7y	108	19	be	be	VB
cord-001142-puj74k7y	108	20	an	an	DT
cord-001142-puj74k7y	108	21	important	important	JJ
cord-001142-puj74k7y	108	22	parameter	parameter	NN
cord-001142-puj74k7y	108	23	for	for	IN
cord-001142-puj74k7y	108	24	polymerase	polymerase	NN
cord-001142-puj74k7y	108	25	binding	bind	VBG
cord-001142-puj74k7y	108	26	[	[	-LRB-
cord-001142-puj74k7y	108	27	32	32	CD
cord-001142-puj74k7y	108	28	]	]	-RRB-
cord-001142-puj74k7y	108	29	.	.	.
cord-001142-puj74k7y	109	1	A	a	DT
cord-001142-puj74k7y	109	2	weaker	weak	JJR
cord-001142-puj74k7y	109	3	distal	distal	JJ
cord-001142-puj74k7y	109	4	panhandle	panhandle	NN
cord-001142-puj74k7y	109	5	for	for	IN
cord-001142-puj74k7y	109	6	5'A/	5'a/	CD
cord-001142-puj74k7y	109	7	3'C(C5U	3'c(c5u	CD
cord-001142-puj74k7y	109	8	)	)	-RRB-
cord-001142-puj74k7y	110	1	(	(	-LRB-
cord-001142-puj74k7y	110	2	score	score	NN
cord-001142-puj74k7y	110	3	7.39	7.39	CD
cord-001142-puj74k7y	110	4	vs	vs	IN
cord-001142-puj74k7y	110	5	10.74	10.74	CD
cord-001142-puj74k7y	110	6	for	for	IN
cord-001142-puj74k7y	110	7	wild	wild	JJ
cord-001142-puj74k7y	110	8	-	-	HYPH
cord-001142-puj74k7y	110	9	type	type	NN
cord-001142-puj74k7y	110	10	)	)	-RRB-
cord-001142-puj74k7y	110	11	could	could	MD
cord-001142-puj74k7y	110	12	facilitate	facilitate	VB
cord-001142-puj74k7y	110	13	polymerase	polymerase	NN
cord-001142-puj74k7y	110	14	binding	binding	NN
cord-001142-puj74k7y	110	15	and/or	and/or	CC
cord-001142-puj74k7y	110	16	transcription	transcription	NN
cord-001142-puj74k7y	110	17	initiation	initiation	NN
cord-001142-puj74k7y	110	18	,	,	,
cord-001142-puj74k7y	110	19	resulting	result	VBG
cord-001142-puj74k7y	110	20	in	in	IN
cord-001142-puj74k7y	110	21	slightly	slightly	RB
cord-001142-puj74k7y	110	22	more	more	JJR
cord-001142-puj74k7y	110	23	NS	NS	NNP
cord-001142-puj74k7y	110	24	mRNA	mrna	NN
cord-001142-puj74k7y	110	25	synthesized	synthesize	VBN
cord-001142-puj74k7y	110	26	.	.	.
cord-001142-puj74k7y	111	1	This	this	DT
cord-001142-puj74k7y	111	2	is	be	VBZ
cord-001142-puj74k7y	111	3	further	further	RB
cord-001142-puj74k7y	111	4	supported	support	VBN
cord-001142-puj74k7y	111	5	by	by	IN
cord-001142-puj74k7y	111	6	the	the	DT
cord-001142-puj74k7y	111	7	results	result	NNS
cord-001142-puj74k7y	111	8	for	for	IN
cord-001142-puj74k7y	111	9	the	the	DT
cord-001142-puj74k7y	111	10	5'C/3'C	5'c/3'c	CD
cord-001142-puj74k7y	111	11	-	-	:
cord-001142-puj74k7y	111	12	proxA	proxa	JJ
cord-001142-puj74k7y	111	13	virus	virus	NN
cord-001142-puj74k7y	111	14	,	,	,
cord-001142-puj74k7y	111	15	whose	whose	WP$
cord-001142-puj74k7y	111	16	distal	distal	JJ
cord-001142-puj74k7y	111	17	panhandle	panhandle	JJ
cord-001142-puj74k7y	111	18	base	base	NN
cord-001142-puj74k7y	111	19	-	-	HYPH
cord-001142-puj74k7y	111	20	pairing	pair	VBG
cord-001142-puj74k7y	111	21	score	score	NN
cord-001142-puj74k7y	111	22	is	be	VBZ
cord-001142-puj74k7y	111	23	identical	identical	JJ
cord-001142-puj74k7y	111	24	to	to	IN
cord-001142-puj74k7y	111	25	that	that	DT
cord-001142-puj74k7y	111	26	of	of	IN
cord-001142-puj74k7y	111	27	wild	wild	JJ
cord-001142-puj74k7y	111	28	-	-	HYPH
cord-001142-puj74k7y	111	29	type	type	NN
cord-001142-puj74k7y	111	30	5'A/3'A	5'a/3'a	NN
cord-001142-puj74k7y	112	1	(	(	-LRB-
cord-001142-puj74k7y	112	2	Figure	figure	NN
cord-001142-puj74k7y	112	3	1	1	CD
cord-001142-puj74k7y	112	4	)	)	-RRB-
cord-001142-puj74k7y	112	5	,	,	,
cord-001142-puj74k7y	112	6	and	and	CC
cord-001142-puj74k7y	112	7	for	for	IN
cord-001142-puj74k7y	112	8	which	which	WDT
cord-001142-puj74k7y	112	9	NS1	ns1	NN
cord-001142-puj74k7y	112	10	expression	expression	NN
cord-001142-puj74k7y	112	11	levels	level	NNS
cord-001142-puj74k7y	112	12	(	(	-LRB-
cord-001142-puj74k7y	112	13	Figure	figure	NN
cord-001142-puj74k7y	112	14	  	  	_SP
cord-001142-puj74k7y	112	15	3B	3B	NNP
cord-001142-puj74k7y	112	16	)	)	-RRB-
cord-001142-puj74k7y	112	17	and	and	CC
cord-001142-puj74k7y	112	18	mRNA	mRNA	NNP
cord-001142-puj74k7y	112	19	/	/	SYM
cord-001142-puj74k7y	112	20	vRNA	vrna	JJ
cord-001142-puj74k7y	112	21	ratios	ratio	NNS
cord-001142-puj74k7y	112	22	(	(	-LRB-
cord-001142-puj74k7y	112	23	Figure	figure	NN
cord-001142-puj74k7y	112	24	3A	3a	CD
cord-001142-puj74k7y	112	25	)	)	-RRB-
cord-001142-puj74k7y	112	26	were	be	VBD
cord-001142-puj74k7y	112	27	similar	similar	JJ
cord-001142-puj74k7y	112	28	to	to	IN
cord-001142-puj74k7y	112	29	those	those	DT
cord-001142-puj74k7y	112	30	obtained	obtain	VBN
cord-001142-puj74k7y	112	31	for	for	IN
cord-001142-puj74k7y	112	32	wild	wild	JJ
cord-001142-puj74k7y	112	33	-	-	HYPH
cord-001142-puj74k7y	112	34	type	type	NN
cord-001142-puj74k7y	112	35	.	.	.
cord-001142-puj74k7y	113	1	The	the	DT
cord-001142-puj74k7y	113	2	major	major	JJ
cord-001142-puj74k7y	113	3	role	role	NN
cord-001142-puj74k7y	113	4	of	of	IN
cord-001142-puj74k7y	113	5	the	the	DT
cord-001142-puj74k7y	113	6	proximal	proximal	JJ
cord-001142-puj74k7y	113	7	panhandle	panhandle	NN
cord-001142-puj74k7y	113	8	in	in	IN
cord-001142-puj74k7y	113	9	type	type	NN
cord-001142-puj74k7y	113	10	specificity	specificity	NN
cord-001142-puj74k7y	113	11	that	that	WDT
cord-001142-puj74k7y	113	12	we	-PRON-	PRP
cord-001142-puj74k7y	113	13	identified	identify	VBD
cord-001142-puj74k7y	113	14	and	and	CC
cord-001142-puj74k7y	113	15	the	the	DT
cord-001142-puj74k7y	113	16	hypothesized	hypothesize	VBN
cord-001142-puj74k7y	113	17	involvement	involvement	NN
cord-001142-puj74k7y	113	18	of	of	IN
cord-001142-puj74k7y	113	19	the	the	DT
cord-001142-puj74k7y	113	20	distal	distal	JJ
cord-001142-puj74k7y	113	21	panhandle	panhandle	NN
cord-001142-puj74k7y	113	22	in	in	IN
cord-001142-puj74k7y	113	23	transcription	transcription	NN
cord-001142-puj74k7y	113	24	need	need	VBP
cord-001142-puj74k7y	113	25	to	to	TO
cord-001142-puj74k7y	113	26	be	be	VB
cord-001142-puj74k7y	113	27	tested	test	VBN
cord-001142-puj74k7y	113	28	on	on	IN
cord-001142-puj74k7y	113	29	the	the	DT
cord-001142-puj74k7y	113	30	other	other	JJ
cord-001142-puj74k7y	113	31	(	(	-LRB-
cord-001142-puj74k7y	113	32	2	2	CD
cord-001142-puj74k7y	113	33	PFU	pfu	NN
cord-001142-puj74k7y	113	34	/	/	SYM
cord-001142-puj74k7y	113	35	cell	cell	NN
cord-001142-puj74k7y	113	36	)	)	-RRB-
cord-001142-puj74k7y	113	37	,	,	,
cord-001142-puj74k7y	113	38	viral	viral	JJ
cord-001142-puj74k7y	113	39	vRNA	vRNA	NNPS
cord-001142-puj74k7y	113	40	and	and	CC
cord-001142-puj74k7y	113	41	mRNA	mRNA	NNP
cord-001142-puj74k7y	113	42	levels	level	NNS
cord-001142-puj74k7y	113	43	for	for	IN
cord-001142-puj74k7y	113	44	each	each	DT
cord-001142-puj74k7y	113	45	segment	segment	NN
cord-001142-puj74k7y	113	46	were	be	VBD
cord-001142-puj74k7y	113	47	evaluated	evaluate	VBN
cord-001142-puj74k7y	113	48	by	by	IN
cord-001142-puj74k7y	113	49	specific	specific	JJ
cord-001142-puj74k7y	113	50	two	two	CD
cord-001142-puj74k7y	113	51	-	-	HYPH
cord-001142-puj74k7y	113	52	step	step	NN
cord-001142-puj74k7y	113	53	RT	RT	NNP
cord-001142-puj74k7y	113	54	-	-	HYPH
cord-001142-puj74k7y	113	55	qPCRs	qPCRs	NNP
cord-001142-puj74k7y	113	56	previously	previously	RB
cord-001142-puj74k7y	113	57	described	describe	VBD
cord-001142-puj74k7y	113	58	[	[	-LRB-
cord-001142-puj74k7y	113	59	23	23	CD
cord-001142-puj74k7y	113	60	]	]	-RRB-
cord-001142-puj74k7y	113	61	.	.	.
cord-001142-puj74k7y	114	1	Results	result	NNS
cord-001142-puj74k7y	114	2	were	be	VBD
cord-001142-puj74k7y	114	3	expressed	express	VBN
cord-001142-puj74k7y	114	4	as	as	IN
cord-001142-puj74k7y	114	5	the	the	DT
cord-001142-puj74k7y	114	6	mean	mean	NN
cord-001142-puj74k7y	114	7	of	of	IN
cord-001142-puj74k7y	114	8	mRNA	mRNA	NNP
cord-001142-puj74k7y	114	9	/	/	SYM
cord-001142-puj74k7y	114	10	vRNA	vrna	JJ
cord-001142-puj74k7y	114	11	Cp	cp	NN
cord-001142-puj74k7y	114	12	(	(	-LRB-
cord-001142-puj74k7y	114	13	crossing	crossing	NN
cord-001142-puj74k7y	114	14	-	-	HYPH
cord-001142-puj74k7y	114	15	point	point	NN
cord-001142-puj74k7y	114	16	)	)	-RRB-
cord-001142-puj74k7y	114	17	ratios	ratio	NNS
cord-001142-puj74k7y	114	18	calculated	calculate	VBN
cord-001142-puj74k7y	114	19	on	on	IN
cord-001142-puj74k7y	114	20	data	datum	NNS
cord-001142-puj74k7y	114	21	obtained	obtain	VBN
cord-001142-puj74k7y	114	22	from	from	IN
cord-001142-puj74k7y	114	23	12	12	CD
cord-001142-puj74k7y	114	24	independent	independent	JJ
cord-001142-puj74k7y	114	25	qPCRs	qPCRs	NNPS
cord-001142-puj74k7y	114	26	(	(	-LRB-
cord-001142-puj74k7y	114	27	corresponding	correspond	VBG
cord-001142-puj74k7y	114	28	to	to	IN
cord-001142-puj74k7y	114	29	two	two	CD
cord-001142-puj74k7y	114	30	infections	infection	NNS
cord-001142-puj74k7y	114	31	,	,	,
cord-001142-puj74k7y	114	32	two	two	CD
cord-001142-puj74k7y	114	33	independent	independent	JJ
cord-001142-puj74k7y	114	34	cDNA	cdna	NN
cord-001142-puj74k7y	114	35	syntheses	synthesis	NNS
cord-001142-puj74k7y	114	36	per	per	IN
cord-001142-puj74k7y	114	37	infection	infection	NN
cord-001142-puj74k7y	114	38	and	and	CC
cord-001142-puj74k7y	114	39	three	three	CD
cord-001142-puj74k7y	114	40	independent	independent	JJ
cord-001142-puj74k7y	114	41	qPCRs	qPCRs	NNPS
cord-001142-puj74k7y	114	42	for	for	IN
cord-001142-puj74k7y	114	43	each	each	DT
cord-001142-puj74k7y	114	44	cDNA	cdna	NN
cord-001142-puj74k7y	114	45	)	)	-RRB-
cord-001142-puj74k7y	114	46	.	.	.
cord-001142-puj74k7y	115	1	Statistics	statistic	NNS
cord-001142-puj74k7y	115	2	were	be	VBD
cord-001142-puj74k7y	115	3	performed	perform	VBN
cord-001142-puj74k7y	115	4	using	use	VBG
cord-001142-puj74k7y	115	5	the	the	DT
cord-001142-puj74k7y	115	6	ANOVA	ANOVA	NNP
cord-001142-puj74k7y	115	7	test	test	NN
cord-001142-puj74k7y	115	8	.	.	.
cord-001142-puj74k7y	116	1	Black	black	JJ
cord-001142-puj74k7y	116	2	bars	bar	NNS
cord-001142-puj74k7y	116	3	:	:	:
cord-001142-puj74k7y	116	4	wild	wild	JJ
cord-001142-puj74k7y	116	5	-	-	HYPH
cord-001142-puj74k7y	116	6	type	type	NN
cord-001142-puj74k7y	116	7	5'A/3'A	5'a/3'a	NN
cord-001142-puj74k7y	116	8	;	;	,
cord-001142-puj74k7y	116	9	hatched	hatch	VBN
cord-001142-puj74k7y	116	10	bars	bar	NNS
cord-001142-puj74k7y	116	11	:	:	:
cord-001142-puj74k7y	116	12	5'A/3'C(C5U	5'a/3'c(c5u	CD
cord-001142-puj74k7y	116	13	)	)	-RRB-
cord-001142-puj74k7y	116	14	;	;	:
cord-001142-puj74k7y	116	15	grey	grey	NNP
cord-001142-puj74k7y	116	16	bars	bar	NNS
cord-001142-puj74k7y	116	17	:	:	:
cord-001142-puj74k7y	116	18	5'C/	5'c/	CD
cord-001142-puj74k7y	116	19	3'C	3'c	CD
cord-001142-puj74k7y	116	20	-	-	HYPH
cord-001142-puj74k7y	116	21	proxA.	proxa.	JJ
cord-001142-puj74k7y	116	22	(	(	-LRB-
cord-001142-puj74k7y	116	23	B	b	NN
cord-001142-puj74k7y	116	24	)	)	-RRB-
cord-001142-puj74k7y	116	25	Levels	level	NNS
cord-001142-puj74k7y	116	26	of	of	IN
cord-001142-puj74k7y	116	27	NP	NP	NNP
cord-001142-puj74k7y	116	28	and	and	CC
cord-001142-puj74k7y	116	29	NS1	ns1	NN
cord-001142-puj74k7y	116	30	proteins	protein	NNS
cord-001142-puj74k7y	116	31	after	after	IN
cord-001142-puj74k7y	116	32	infection	infection	NN
cord-001142-puj74k7y	116	33	with	with	IN
cord-001142-puj74k7y	116	34	the	the	DT
cord-001142-puj74k7y	116	35	rescued	rescue	VBN
cord-001142-puj74k7y	116	36	influenza	influenza	NN
cord-001142-puj74k7y	116	37	A	a	DT
cord-001142-puj74k7y	116	38	viruses	virus	NNS
cord-001142-puj74k7y	116	39	.	.	.
cord-001142-puj74k7y	117	1	Four	four	CD
cord-001142-puj74k7y	117	2	hours	hour	NNS
cord-001142-puj74k7y	117	3	after	after	IN
cord-001142-puj74k7y	117	4	MDCK	MDCK	NNP
cord-001142-puj74k7y	117	5	infection	infection	NN
cord-001142-puj74k7y	117	6	at	at	IN
cord-001142-puj74k7y	117	7	a	a	DT
cord-001142-puj74k7y	117	8	high	high	JJ
cord-001142-puj74k7y	117	9	m.o.i	m.o.i	NN
cord-001142-puj74k7y	117	10	.	.	.
cord-001142-puj74k7y	118	1	(	(	-LRB-
cord-001142-puj74k7y	118	2	2	2	CD
cord-001142-puj74k7y	118	3	PFU	pfu	NN
cord-001142-puj74k7y	118	4	/	/	SYM
cord-001142-puj74k7y	118	5	cell	cell	NN
cord-001142-puj74k7y	118	6	)	)	-RRB-
cord-001142-puj74k7y	118	7	,	,	,
cord-001142-puj74k7y	118	8	cell	cell	NN
cord-001142-puj74k7y	118	9	lysates	lysate	NNS
cord-001142-puj74k7y	118	10	were	be	VBD
cord-001142-puj74k7y	118	11	analyzed	analyze	VBN
cord-001142-puj74k7y	118	12	by	by	IN
cord-001142-puj74k7y	118	13	Western	western	JJ
cord-001142-puj74k7y	118	14	-	-	HYPH
cord-001142-puj74k7y	118	15	blot	blot	NN
cord-001142-puj74k7y	118	16	for	for	IN
cord-001142-puj74k7y	118	17	viral	viral	JJ
cord-001142-puj74k7y	118	18	NS1	NS1	NNS
cord-001142-puj74k7y	118	19	and	and	CC
cord-001142-puj74k7y	118	20	NP	NP	NNP
cord-001142-puj74k7y	118	21	proteins	protein	NNS
cord-001142-puj74k7y	118	22	and	and	CC
cord-001142-puj74k7y	118	23	for	for	IN
cord-001142-puj74k7y	118	24	β	β	NN
cord-001142-puj74k7y	118	25	-	-	HYPH
cord-001142-puj74k7y	118	26	actin	actin	NN
cord-001142-puj74k7y	118	27	as	as	IN
cord-001142-puj74k7y	118	28	cellular	cellular	JJ
cord-001142-puj74k7y	118	29	control	control	NN
cord-001142-puj74k7y	118	30	.	.	.
cord-001142-puj74k7y	119	1	Following	follow	VBG
cord-001142-puj74k7y	119	2	chemiluminescence	chemiluminescence	NN
cord-001142-puj74k7y	119	3	acquisition	acquisition	NN
cord-001142-puj74k7y	119	4	with	with	IN
cord-001142-puj74k7y	119	5	a	a	DT
cord-001142-puj74k7y	119	6	G.	G.	NNP
cord-001142-puj74k7y	119	7	Box	Box	NNP
cord-001142-puj74k7y	119	8	(	(	-LRB-
cord-001142-puj74k7y	119	9	SYNGENE	SYNGENE	NNP
cord-001142-puj74k7y	119	10	,	,	,
cord-001142-puj74k7y	119	11	Cambridge	Cambridge	NNP
cord-001142-puj74k7y	119	12	,	,	,
cord-001142-puj74k7y	119	13	UK	UK	NNP
cord-001142-puj74k7y	119	14	)	)	-RRB-
cord-001142-puj74k7y	119	15	,	,	,
cord-001142-puj74k7y	119	16	band	band	NN
cord-001142-puj74k7y	119	17	densities	density	NNS
cord-001142-puj74k7y	119	18	for	for	IN
cord-001142-puj74k7y	119	19	viral	viral	JJ
cord-001142-puj74k7y	119	20	proteins	protein	NNS
cord-001142-puj74k7y	119	21	were	be	VBD
cord-001142-puj74k7y	119	22	determined	determine	VBN
cord-001142-puj74k7y	119	23	using	use	VBG
cord-001142-puj74k7y	119	24	GeneTools	GeneTools	NNP
cord-001142-puj74k7y	119	25	software	software	NN
cord-001142-puj74k7y	119	26	(	(	-LRB-
cord-001142-puj74k7y	119	27	SYNGENE	SYNGENE	NNP
cord-001142-puj74k7y	119	28	,	,	,
cord-001142-puj74k7y	119	29	Cambridge	Cambridge	NNP
cord-001142-puj74k7y	119	30	,	,	,
cord-001142-puj74k7y	119	31	UK	UK	NNP
cord-001142-puj74k7y	119	32	)	)	-RRB-
cord-001142-puj74k7y	119	33	,	,	,
cord-001142-puj74k7y	119	34	and	and	CC
cord-001142-puj74k7y	119	35	were	be	VBD
cord-001142-puj74k7y	119	36	used	use	VBN
cord-001142-puj74k7y	119	37	to	to	TO
cord-001142-puj74k7y	119	38	calculate	calculate	VB
cord-001142-puj74k7y	119	39	NS1	NS1	NNP
cord-001142-puj74k7y	119	40	/	/	SYM
cord-001142-puj74k7y	119	41	NP	NP	NNP
cord-001142-puj74k7y	119	42	ratios	ratio	NNS
cord-001142-puj74k7y	119	43	for	for	IN
cord-001142-puj74k7y	119	44	each	each	DT
cord-001142-puj74k7y	119	45	virus	virus	NN
cord-001142-puj74k7y	119	46	.	.	.
cord-001142-puj74k7y	120	1	Due	due	IN
cord-001142-puj74k7y	120	2	to	to	IN
cord-001142-puj74k7y	120	3	the	the	DT
cord-001142-puj74k7y	120	4	high	high	JJ
cord-001142-puj74k7y	120	5	levels	level	NNS
cord-001142-puj74k7y	120	6	of	of	IN
cord-001142-puj74k7y	120	7	viral	viral	JJ
cord-001142-puj74k7y	120	8	protein	protein	NN
cord-001142-puj74k7y	120	9	expression	expression	NN
cord-001142-puj74k7y	120	10	during	during	IN
cord-001142-puj74k7y	120	11	infection	infection	NN
cord-001142-puj74k7y	120	12	,	,	,
cord-001142-puj74k7y	120	13	protein	protein	NN
cord-001142-puj74k7y	120	14	extracts	extract	NNS
cord-001142-puj74k7y	120	15	were	be	VBD
cord-001142-puj74k7y	120	16	diluted	dilute	VBN
cord-001142-puj74k7y	120	17	in	in	IN
cord-001142-puj74k7y	120	18	uninfected	uninfected	JJ
cord-001142-puj74k7y	120	19	cellular	cellular	JJ
cord-001142-puj74k7y	120	20	extracts	extract	NNS
cord-001142-puj74k7y	120	21	prior	prior	RB
cord-001142-puj74k7y	120	22	to	to	IN
cord-001142-puj74k7y	120	23	electrophoresis	electrophoresis	NN
cord-001142-puj74k7y	120	24	.	.	.
cord-001142-puj74k7y	121	1	Results	result	NNS
cord-001142-puj74k7y	121	2	are	be	VBP
cord-001142-puj74k7y	121	3	from	from	IN
cord-001142-puj74k7y	121	4	one	one	CD
cord-001142-puj74k7y	121	5	representative	representative	JJ
cord-001142-puj74k7y	121	6	assay	assay	NN
cord-001142-puj74k7y	121	7	out	out	IN
cord-001142-puj74k7y	121	8	of	of	IN
cord-001142-puj74k7y	121	9	three	three	CD
cord-001142-puj74k7y	121	10	independent	independent	JJ
cord-001142-puj74k7y	121	11	infections	infection	NNS
cord-001142-puj74k7y	121	12	.	.	.
cord-001142-puj74k7y	122	1	doi	doi	NNP
cord-001142-puj74k7y	122	2	:	:	:
cord-001142-puj74k7y	122	3	10.1371	10.1371	CD
cord-001142-puj74k7y	122	4	/	/	SYM
cord-001142-puj74k7y	122	5	journal.pone.0081550.g003	journal.pone.0081550.g003	NNP
cord-001142-puj74k7y	122	6	segments	segment	NNS
cord-001142-puj74k7y	122	7	.	.	.
cord-001142-puj74k7y	123	1	It	-PRON-	PRP
cord-001142-puj74k7y	123	2	is	be	VBZ
cord-001142-puj74k7y	123	3	interesting	interesting	JJ
cord-001142-puj74k7y	123	4	to	to	TO
cord-001142-puj74k7y	123	5	note	note	VB
cord-001142-puj74k7y	123	6	that	that	DT
cord-001142-puj74k7y	123	7	comparison	comparison	NN
cord-001142-puj74k7y	123	8	of	of	IN
cord-001142-puj74k7y	123	9	the	the	DT
cord-001142-puj74k7y	123	10	distal	distal	JJ
cord-001142-puj74k7y	123	11	panhandles	panhandle	NNS
cord-001142-puj74k7y	123	12	formed	form	VBN
cord-001142-puj74k7y	123	13	by	by	IN
cord-001142-puj74k7y	123	14	the	the	DT
cord-001142-puj74k7y	123	15	extremities	extremity	NNS
cord-001142-puj74k7y	123	16	of	of	IN
cord-001142-puj74k7y	123	17	each	each	DT
cord-001142-puj74k7y	123	18	segment	segment	NN
cord-001142-puj74k7y	123	19	for	for	IN
cord-001142-puj74k7y	123	20	a	a	DT
cord-001142-puj74k7y	123	21	given	give	VBN
cord-001142-puj74k7y	123	22	virus	virus	NN
cord-001142-puj74k7y	123	23	shows	show	VBZ
cord-001142-puj74k7y	123	24	differences	difference	NNS
cord-001142-puj74k7y	123	25	in	in	IN
cord-001142-puj74k7y	123	26	base	base	NN
cord-001142-puj74k7y	123	27	-	-	HYPH
cord-001142-puj74k7y	123	28	pairing	pair	VBG
cord-001142-puj74k7y	123	29	scores	score	NNS
cord-001142-puj74k7y	123	30	(	(	-LRB-
cord-001142-puj74k7y	123	31	data	datum	NNS
cord-001142-puj74k7y	123	32	not	not	RB
cord-001142-puj74k7y	123	33	shown	show	VBN
cord-001142-puj74k7y	123	34	)	)	-RRB-
cord-001142-puj74k7y	123	35	.	.	.
cord-001142-puj74k7y	124	1	In	in	IN
cord-001142-puj74k7y	124	2	particular	particular	JJ
cord-001142-puj74k7y	124	3	,	,	,
cord-001142-puj74k7y	124	4	regardless	regardless	RB
cord-001142-puj74k7y	124	5	of	of	IN
cord-001142-puj74k7y	124	6	the	the	DT
cord-001142-puj74k7y	124	7	influenza	influenza	NN
cord-001142-puj74k7y	124	8	A	a	DT
cord-001142-puj74k7y	124	9	virus	virus	NN
cord-001142-puj74k7y	124	10	subtype	subtype	NN
cord-001142-puj74k7y	124	11	,	,	,
cord-001142-puj74k7y	124	12	the	the	DT
cord-001142-puj74k7y	124	13	distal	distal	JJ
cord-001142-puj74k7y	124	14	panhandle	panhandle	NN
cord-001142-puj74k7y	124	15	of	of	IN
cord-001142-puj74k7y	124	16	the	the	DT
cord-001142-puj74k7y	124	17	HA	HA	NNP
cord-001142-puj74k7y	124	18	segment	segment	NN
cord-001142-puj74k7y	124	19	was	be	VBD
cord-001142-puj74k7y	124	20	systematically	systematically	RB
cord-001142-puj74k7y	124	21	the	the	DT
cord-001142-puj74k7y	124	22	weakest	weak	JJS
cord-001142-puj74k7y	124	23	one	one	CD
cord-001142-puj74k7y	124	24	.	.	.
cord-001142-puj74k7y	125	1	It	-PRON-	PRP
cord-001142-puj74k7y	125	2	would	would	MD
cord-001142-puj74k7y	125	3	be	be	VB
cord-001142-puj74k7y	125	4	interesting	interesting	JJ
cord-001142-puj74k7y	125	5	to	to	TO
cord-001142-puj74k7y	125	6	determine	determine	VB
cord-001142-puj74k7y	125	7	to	to	TO
cord-001142-puj74k7y	125	8	what	what	WDT
cord-001142-puj74k7y	125	9	extent	extent	NN
cord-001142-puj74k7y	125	10	the	the	DT
cord-001142-puj74k7y	125	11	distal	distal	JJ
cord-001142-puj74k7y	125	12	panhandle	panhandle	JJ
cord-001142-puj74k7y	125	13	base	base	NN
cord-001142-puj74k7y	125	14	-	-	HYPH
cord-001142-puj74k7y	125	15	pairing	pair	VBG
cord-001142-puj74k7y	125	16	strength	strength	NN
cord-001142-puj74k7y	125	17	may	may	MD
cord-001142-puj74k7y	125	18	have	have	VB
cord-001142-puj74k7y	125	19	an	an	DT
cord-001142-puj74k7y	125	20	impact	impact	NN
cord-001142-puj74k7y	125	21	on	on	IN
cord-001142-puj74k7y	125	22	HA	HA	NNP
cord-001142-puj74k7y	125	23	expression	expression	NN
cord-001142-puj74k7y	125	24	levels	level	NNS
cord-001142-puj74k7y	125	25	and	and	CC
cord-001142-puj74k7y	125	26	consequently	consequently	RB
cord-001142-puj74k7y	125	27	influence	influence	VBP
cord-001142-puj74k7y	125	28	the	the	DT
cord-001142-puj74k7y	125	29	HA	HA	NNP
cord-001142-puj74k7y	125	30	/	/	SYM
cord-001142-puj74k7y	125	31	NA	NA	NNP
cord-001142-puj74k7y	125	32	molecule	molecule	NN
cord-001142-puj74k7y	125	33	ratio	ratio	NN
cord-001142-puj74k7y	125	34	found	find	VBN
cord-001142-puj74k7y	125	35	at	at	IN
cord-001142-puj74k7y	125	36	the	the	DT
cord-001142-puj74k7y	125	37	virion	virion	NN
cord-001142-puj74k7y	125	38	surface	surface	NN
cord-001142-puj74k7y	125	39	[	[	-LRB-
cord-001142-puj74k7y	125	40	33	33	CD
cord-001142-puj74k7y	125	41	]	]	-RRB-
cord-001142-puj74k7y	125	42	.	.	.
cord-001142-puj74k7y	126	1	Our	-PRON-	PRP$
cord-001142-puj74k7y	126	2	approach	approach	NN
cord-001142-puj74k7y	126	3	to	to	TO
cord-001142-puj74k7y	126	4	understand	understand	VB
cord-001142-puj74k7y	126	5	what	what	WP
cord-001142-puj74k7y	126	6	in	in	IN
cord-001142-puj74k7y	126	7	the	the	DT
cord-001142-puj74k7y	126	8	NS	NS	NNP
cord-001142-puj74k7y	126	9	segment	segment	NN
cord-001142-puj74k7y	126	10	noncoding	noncoding	JJ
cord-001142-puj74k7y	126	11	regions	region	NNS
cord-001142-puj74k7y	126	12	was	be	VBD
cord-001142-puj74k7y	126	13	important	important	JJ
cord-001142-puj74k7y	126	14	for	for	IN
cord-001142-puj74k7y	126	15	type	type	NN
cord-001142-puj74k7y	126	16	specificity	specificity	NN
cord-001142-puj74k7y	126	17	by	by	IN
cord-001142-puj74k7y	126	18	attempting	attempt	VBG
cord-001142-puj74k7y	126	19	to	to	TO
cord-001142-puj74k7y	126	20	rescue	rescue	VB
cord-001142-puj74k7y	126	21	type	type	NN
cord-001142-puj74k7y	126	22	A	a	NN
cord-001142-puj74k7y	126	23	and	and	CC
cord-001142-puj74k7y	126	24	C	c	NN
cord-001142-puj74k7y	126	25	influenza	influenza	NN
cord-001142-puj74k7y	126	26	viruses	virus	NNS
cord-001142-puj74k7y	126	27	with	with	IN
cord-001142-puj74k7y	126	28	chimeric	chimeric	JJ
cord-001142-puj74k7y	126	29	NC	NC	NNP
cord-001142-puj74k7y	126	30	sequences	sequence	NNS
cord-001142-puj74k7y	126	31	in	in	IN
cord-001142-puj74k7y	126	32	the	the	DT
cord-001142-puj74k7y	126	33	NS	NS	NNP
cord-001142-puj74k7y	126	34	segment	segment	NN
cord-001142-puj74k7y	126	35	unraveled	unravel	VBD
cord-001142-puj74k7y	126	36	the	the	DT
cord-001142-puj74k7y	126	37	requirement	requirement	NN
cord-001142-puj74k7y	126	38	for	for	IN
cord-001142-puj74k7y	126	39	a	a	DT
cord-001142-puj74k7y	126	40	type	type	NN
cord-001142-puj74k7y	126	41	specific	specific	JJ
cord-001142-puj74k7y	126	42	proximal	proximal	JJ
cord-001142-puj74k7y	126	43	panhandle	panhandle	NN
cord-001142-puj74k7y	126	44	and	and	CC
cord-001142-puj74k7y	126	45	the	the	DT
cord-001142-puj74k7y	126	46	role	role	NN
cord-001142-puj74k7y	126	47	of	of	IN
cord-001142-puj74k7y	126	48	the	the	DT
cord-001142-puj74k7y	126	49	distal	distal	JJ
cord-001142-puj74k7y	126	50	panhandle	panhandle	NN
cord-001142-puj74k7y	126	51	in	in	IN
cord-001142-puj74k7y	126	52	regulation	regulation	NN
cord-001142-puj74k7y	126	53	of	of	IN
cord-001142-puj74k7y	126	54	transcription	transcription	NN
cord-001142-puj74k7y	126	55	.	.	.
cord-001142-puj74k7y	127	1	Our	-PRON-	PRP$
cord-001142-puj74k7y	127	2	results	result	NNS
cord-001142-puj74k7y	127	3	thus	thus	RB
cord-001142-puj74k7y	127	4	provide	provide	VBP
cord-001142-puj74k7y	127	5	useful	useful	JJ
cord-001142-puj74k7y	127	6	information	information	NN
cord-001142-puj74k7y	127	7	to	to	TO
cord-001142-puj74k7y	127	8	improve	improve	VB
cord-001142-puj74k7y	127	9	our	-PRON-	PRP$
cord-001142-puj74k7y	127	10	understanding	understanding	NN
cord-001142-puj74k7y	127	11	of	of	IN
cord-001142-puj74k7y	127	12	influenza	influenza	NN
cord-001142-puj74k7y	127	13	A	a	DT
cord-001142-puj74k7y	127	14	virus	virus	NN
cord-001142-puj74k7y	127	15	biology	biology	NN
cord-001142-puj74k7y	127	16	.	.	.
cord-001142-puj74k7y	128	1	Evolution	evolution	NN
cord-001142-puj74k7y	128	2	and	and	CC
cord-001142-puj74k7y	128	3	ecology	ecology	NN
cord-001142-puj74k7y	128	4	of	of	IN
cord-001142-puj74k7y	128	5	influenza	influenza	NN
cord-001142-puj74k7y	128	6	A	a	DT
cord-001142-puj74k7y	128	7	viruses	virus	NNS
cord-001142-puj74k7y	129	1	Orthomyxoviridae	Orthomyxoviridae	NNP
cord-001142-puj74k7y	129	2	:	:	:
cord-001142-puj74k7y	130	1	The	the	DT
cord-001142-puj74k7y	130	2	viruses	virus	NNS
cord-001142-puj74k7y	130	3	and	and	CC
cord-001142-puj74k7y	130	4	their	-PRON-	PRP$
cord-001142-puj74k7y	130	5	replication	replication	NN
cord-001142-puj74k7y	130	6	Mutations	mutation	NNS
cord-001142-puj74k7y	130	7	in	in	IN
cord-001142-puj74k7y	130	8	the	the	DT
cord-001142-puj74k7y	130	9	nonconserved	nonconserved	JJ
cord-001142-puj74k7y	130	10	noncoding	noncoding	JJ
cord-001142-puj74k7y	130	11	sequences	sequence	NNS
cord-001142-puj74k7y	130	12	of	of	IN
cord-001142-puj74k7y	130	13	the	the	DT
cord-001142-puj74k7y	130	14	influenza	influenza	NN
cord-001142-puj74k7y	130	15	A	a	DT
cord-001142-puj74k7y	130	16	virus	virus	NN
cord-001142-puj74k7y	130	17	segments	segment	NNS
cord-001142-puj74k7y	130	18	affect	affect	VBP
cord-001142-puj74k7y	130	19	viral	viral	JJ
cord-001142-puj74k7y	130	20	vRNA	vRNA	HYPH
cord-001142-puj74k7y	130	21	formation	formation	NN
cord-001142-puj74k7y	130	22	Non	non	JJ
cord-001142-puj74k7y	130	23	coding	code	VBG
cord-001142-puj74k7y	130	24	extremities	extremity	NNS
cord-001142-puj74k7y	130	25	of	of	IN
cord-001142-puj74k7y	130	26	the	the	DT
cord-001142-puj74k7y	130	27	seven	seven	CD
cord-001142-puj74k7y	130	28	influenza	influenza	NN
cord-001142-puj74k7y	130	29	virus	virus	NN
cord-001142-puj74k7y	130	30	type	type	NN
cord-001142-puj74k7y	130	31	C	C	NNP
cord-001142-puj74k7y	130	32	vRNA	vRNA	HYPH
cord-001142-puj74k7y	130	33	segments	segment	NNS
cord-001142-puj74k7y	130	34	:	:	:
cord-001142-puj74k7y	130	35	effect	effect	VB
cord-001142-puj74k7y	130	36	on	on	IN
cord-001142-puj74k7y	130	37	transcription	transcription	NN
cord-001142-puj74k7y	130	38	and	and	CC
cord-001142-puj74k7y	130	39	replication	replication	NN
cord-001142-puj74k7y	130	40	by	by	IN
cord-001142-puj74k7y	130	41	the	the	DT
cord-001142-puj74k7y	130	42	type	type	NN
cord-001142-puj74k7y	130	43	C	c	NN
cord-001142-puj74k7y	130	44	and	and	CC
cord-001142-puj74k7y	130	45	type	type	VB
cord-001142-puj74k7y	130	46	A	a	NN
cord-001142-puj74k7y	131	1	polymerase	polymerase	NNP
cord-001142-puj74k7y	132	1	complexes	complex	NNS
cord-001142-puj74k7y	132	2	Molecular	Molecular	NNP
cord-001142-puj74k7y	132	3	studies	study	NNS
cord-001142-puj74k7y	132	4	of	of	IN
cord-001142-puj74k7y	132	5	influenza	influenza	NN
cord-001142-puj74k7y	132	6	B	B	NNP
cord-001142-puj74k7y	132	7	virus	virus	NN
cord-001142-puj74k7y	132	8	in	in	IN
cord-001142-puj74k7y	132	9	the	the	DT
cord-001142-puj74k7y	132	10	reverse	reverse	JJ
cord-001142-puj74k7y	132	11	genetics	genetics	NN
cord-001142-puj74k7y	132	12	era	era	NN
cord-001142-puj74k7y	133	1	The	the	DT
cord-001142-puj74k7y	133	2	3	3	CD
cord-001142-puj74k7y	133	3	'	'	''
cord-001142-puj74k7y	133	4	and	and	CC
cord-001142-puj74k7y	133	5	5'-terminal	5'-terminal	DT
cord-001142-puj74k7y	133	6	sequences	sequence	NNS
cord-001142-puj74k7y	133	7	of	of	IN
cord-001142-puj74k7y	133	8	influenza	influenza	NN
cord-001142-puj74k7y	133	9	A	a	NN
cord-001142-puj74k7y	133	10	,	,	,
cord-001142-puj74k7y	133	11	B	B	NNP
cord-001142-puj74k7y	133	12	and	and	CC
cord-001142-puj74k7y	133	13	C	C	NNP
cord-001142-puj74k7y	133	14	virus	virus	NN
cord-001142-puj74k7y	133	15	RNA	rna	NN
cord-001142-puj74k7y	133	16	segments	segment	NNS
cord-001142-puj74k7y	133	17	are	be	VBP
cord-001142-puj74k7y	133	18	highly	highly	RB
cord-001142-puj74k7y	133	19	conserved	conserve	VBN
cord-001142-puj74k7y	133	20	and	and	CC
cord-001142-puj74k7y	133	21	show	show	VBP
cord-001142-puj74k7y	133	22	partial	partial	JJ
cord-001142-puj74k7y	133	23	inverted	inverted	JJ
cord-001142-puj74k7y	133	24	complementarity	complementarity	NN
cord-001142-puj74k7y	133	25	5	5	CD
cord-001142-puj74k7y	133	26	'	'	''
cord-001142-puj74k7y	133	27	and	and	CC
cord-001142-puj74k7y	133	28	3	3	CD
cord-001142-puj74k7y	133	29	'	'	POS
cord-001142-puj74k7y	133	30	terminal	terminal	JJ
cord-001142-puj74k7y	133	31	nucleotide	nucleotide	JJ
cord-001142-puj74k7y	133	32	sequences	sequence	NNS
cord-001142-puj74k7y	133	33	of	of	IN
cord-001142-puj74k7y	133	34	the	the	DT
cord-001142-puj74k7y	133	35	RNA	RNA	NNP
cord-001142-puj74k7y	133	36	genome	genome	NN
cord-001142-puj74k7y	133	37	segments	segment	NNS
cord-001142-puj74k7y	133	38	of	of	IN
cord-001142-puj74k7y	133	39	influenza	influenza	NN
cord-001142-puj74k7y	133	40	virus	virus	NN
cord-001142-puj74k7y	133	41	Segment	segment	NN
cord-001142-puj74k7y	133	42	-	-	HYPH
cord-001142-puj74k7y	133	43	specific	specific	JJ
cord-001142-puj74k7y	133	44	and	and	CC
cord-001142-puj74k7y	133	45	common	common	JJ
cord-001142-puj74k7y	133	46	nucleotide	nucleotide	JJ
cord-001142-puj74k7y	133	47	sequences	sequence	NNS
cord-001142-puj74k7y	133	48	in	in	IN
cord-001142-puj74k7y	133	49	the	the	DT
cord-001142-puj74k7y	133	50	noncoding	noncoding	JJ
cord-001142-puj74k7y	133	51	regions	region	NNS
cord-001142-puj74k7y	133	52	of	of	IN
cord-001142-puj74k7y	133	53	influenza	influenza	NN
cord-001142-puj74k7y	133	54	B	b	NN
cord-001142-puj74k7y	133	55	virus	virus	NN
cord-001142-puj74k7y	133	56	genome	genome	NN
cord-001142-puj74k7y	133	57	RNAs	rna	NNS
cord-001142-puj74k7y	134	1	Orthomyxovirus	Orthomyxovirus	NNP
cord-001142-puj74k7y	134	2	replication	replication	NN
cord-001142-puj74k7y	134	3	,	,	,
cord-001142-puj74k7y	134	4	transcription	transcription	NN
cord-001142-puj74k7y	134	5	,	,	,
cord-001142-puj74k7y	134	6	and	and	CC
cord-001142-puj74k7y	134	7	polyadenylation	polyadenylation	NN
cord-001142-puj74k7y	134	8	Differential	differential	NN
cord-001142-puj74k7y	134	9	effect	effect	NN
cord-001142-puj74k7y	134	10	of	of	IN
cord-001142-puj74k7y	134	11	nucleotide	nucleotide	JJ
cord-001142-puj74k7y	134	12	substitutions	substitution	NNS
cord-001142-puj74k7y	134	13	in	in	IN
cord-001142-puj74k7y	134	14	the	the	DT
cord-001142-puj74k7y	134	15	3	3	CD
cord-001142-puj74k7y	134	16	'	'	POS
cord-001142-puj74k7y	134	17	arm	arm	NN
cord-001142-puj74k7y	134	18	of	of	IN
cord-001142-puj74k7y	134	19	the	the	DT
cord-001142-puj74k7y	134	20	influenza	influenza	NN
cord-001142-puj74k7y	134	21	A	a	DT
cord-001142-puj74k7y	134	22	virus	virus	NN
cord-001142-puj74k7y	134	23	vRNA	vrna	IN
cord-001142-puj74k7y	134	24	promoter	promoter	NN
cord-001142-puj74k7y	134	25	on	on	IN
cord-001142-puj74k7y	134	26	transcription	transcription	NN
cord-001142-puj74k7y	134	27	/	/	SYM
cord-001142-puj74k7y	134	28	replication	replication	NN
cord-001142-puj74k7y	134	29	by	by	IN
cord-001142-puj74k7y	134	30	avian	avian	JJ
cord-001142-puj74k7y	134	31	and	and	CC
cord-001142-puj74k7y	134	32	human	human	JJ
cord-001142-puj74k7y	134	33	polymerase	polymerase	NN
cord-001142-puj74k7y	134	34	complexes	complex	NNS
cord-001142-puj74k7y	134	35	is	be	VBZ
cord-001142-puj74k7y	134	36	related	relate	VBN
cord-001142-puj74k7y	134	37	to	to	IN
cord-001142-puj74k7y	134	38	the	the	DT
cord-001142-puj74k7y	134	39	nature	nature	NN
cord-001142-puj74k7y	134	40	of	of	IN
cord-001142-puj74k7y	134	41	PB2	PB2	NNP
cord-001142-puj74k7y	134	42	amino	amino	NNS
cord-001142-puj74k7y	134	43	acid	acid	VBP
cord-001142-puj74k7y	134	44	627	627	CD
cord-001142-puj74k7y	135	1	The	the	DT
cord-001142-puj74k7y	135	2	influenza	influenza	NN
cord-001142-puj74k7y	135	3	virus	virus	NN
cord-001142-puj74k7y	135	4	panhandle	panhandle	NNP
cord-001142-puj74k7y	135	5	is	be	VBZ
cord-001142-puj74k7y	135	6	involved	involve	VBN
cord-001142-puj74k7y	135	7	in	in	IN
cord-001142-puj74k7y	135	8	the	the	DT
cord-001142-puj74k7y	135	9	initiation	initiation	NN
cord-001142-puj74k7y	135	10	of	of	IN
cord-001142-puj74k7y	135	11	transcription	transcription	NN
cord-001142-puj74k7y	135	12	Characterization	characterization	NN
cord-001142-puj74k7y	135	13	of	of	IN
cord-001142-puj74k7y	135	14	the	the	DT
cord-001142-puj74k7y	135	15	RNA	RNA	NNP
cord-001142-puj74k7y	135	16	-	-	HYPH
cord-001142-puj74k7y	135	17	fork	fork	NN
cord-001142-puj74k7y	135	18	model	model	NN
cord-001142-puj74k7y	135	19	of	of	IN
cord-001142-puj74k7y	135	20	virion	virion	NN
cord-001142-puj74k7y	135	21	RNA	RNA	NNP
cord-001142-puj74k7y	135	22	in	in	IN
cord-001142-puj74k7y	135	23	the	the	DT
cord-001142-puj74k7y	135	24	initiation	initiation	NN
cord-001142-puj74k7y	135	25	of	of	IN
cord-001142-puj74k7y	135	26	transcription	transcription	NN
cord-001142-puj74k7y	135	27	in	in	IN
cord-001142-puj74k7y	135	28	influenza	influenza	NN
cord-001142-puj74k7y	135	29	A	a	DT
cord-001142-puj74k7y	135	30	virus	virus	NN
cord-001142-puj74k7y	136	1	Genomic	genomic	JJ
cord-001142-puj74k7y	136	2	RNAs	rna	NNS
cord-001142-puj74k7y	136	3	of	of	IN
cord-001142-puj74k7y	136	4	influenza	influenza	NN
cord-001142-puj74k7y	136	5	viruses	virus	NNS
cord-001142-puj74k7y	136	6	are	be	VBP
cord-001142-puj74k7y	136	7	held	hold	VBN
cord-001142-puj74k7y	136	8	in	in	IN
cord-001142-puj74k7y	136	9	a	a	DT
cord-001142-puj74k7y	136	10	circular	circular	JJ
cord-001142-puj74k7y	136	11	conformation	conformation	NN
cord-001142-puj74k7y	136	12	in	in	IN
cord-001142-puj74k7y	136	13	virions	virion	NNS
cord-001142-puj74k7y	136	14	and	and	CC
cord-001142-puj74k7y	136	15	in	in	IN
cord-001142-puj74k7y	136	16	infected	infected	JJ
cord-001142-puj74k7y	136	17	cells	cell	NNS
cord-001142-puj74k7y	136	18	by	by	IN
cord-001142-puj74k7y	136	19	a	a	DT
cord-001142-puj74k7y	136	20	terminal	terminal	JJ
cord-001142-puj74k7y	136	21	panhandle	panhandle	NNP
cord-001142-puj74k7y	136	22	Structure	Structure	NNP
cord-001142-puj74k7y	136	23	of	of	IN
cord-001142-puj74k7y	136	24	influenza	influenza	NNP
cord-001142-puj74k7y	136	25	virus	virus	NNP
cord-001142-puj74k7y	136	26	panhandle	panhandle	NNP
cord-001142-puj74k7y	136	27	RNA	RNA	NNP
cord-001142-puj74k7y	136	28	studied	study	VBN
cord-001142-puj74k7y	136	29	by	by	IN
cord-001142-puj74k7y	136	30	NMR	NMR	NNP
cord-001142-puj74k7y	136	31	spectroscopy	spectroscopy	NN
cord-001142-puj74k7y	136	32	and	and	CC
cord-001142-puj74k7y	136	33	molecular	molecular	JJ
cord-001142-puj74k7y	136	34	modeling	model	VBG
cord-001142-puj74k7y	136	35	Nucleotides	nucleotide	NNS
cord-001142-puj74k7y	136	36	at	at	IN
cord-001142-puj74k7y	136	37	the	the	DT
cord-001142-puj74k7y	136	38	extremities	extremity	NNS
cord-001142-puj74k7y	136	39	of	of	IN
cord-001142-puj74k7y	136	40	the	the	DT
cord-001142-puj74k7y	136	41	viral	viral	JJ
cord-001142-puj74k7y	136	42	RNA	RNA	NNP
cord-001142-puj74k7y	136	43	of	of	IN
cord-001142-puj74k7y	136	44	influenza	influenza	NN
cord-001142-puj74k7y	136	45	C	C	NNP
cord-001142-puj74k7y	136	46	virus	virus	NN
cord-001142-puj74k7y	136	47	are	be	VBP
cord-001142-puj74k7y	136	48	involved	involve	VBN
cord-001142-puj74k7y	136	49	in	in	IN
cord-001142-puj74k7y	136	50	typespecific	typespecific	JJ
cord-001142-puj74k7y	136	51	interactions	interaction	NNS
cord-001142-puj74k7y	136	52	with	with	IN
cord-001142-puj74k7y	136	53	the	the	DT
cord-001142-puj74k7y	136	54	polymerase	polymerase	NN
cord-001142-puj74k7y	136	55	complex	complex	JJ
cord-001142-puj74k7y	136	56	Mutational	Mutational	NNP
cord-001142-puj74k7y	136	57	analysis	analysis	NN
cord-001142-puj74k7y	136	58	of	of	IN
cord-001142-puj74k7y	136	59	the	the	DT
cord-001142-puj74k7y	136	60	influenza	influenza	NN
cord-001142-puj74k7y	136	61	virus	virus	NN
cord-001142-puj74k7y	136	62	cRNA	crna	NN
cord-001142-puj74k7y	136	63	promoter	promoter	NN
cord-001142-puj74k7y	136	64	and	and	CC
cord-001142-puj74k7y	136	65	identification	identification	NN
cord-001142-puj74k7y	136	66	of	of	IN
cord-001142-puj74k7y	136	67	nucleotides	nucleotide	NNS
cord-001142-puj74k7y	136	68	critical	critical	JJ
cord-001142-puj74k7y	136	69	for	for	IN
cord-001142-puj74k7y	136	70	replication	replication	NN
cord-001142-puj74k7y	136	71	Promoter	Promoter	NNP
cord-001142-puj74k7y	136	72	elements	element	NNS
cord-001142-puj74k7y	136	73	in	in	IN
cord-001142-puj74k7y	136	74	the	the	DT
cord-001142-puj74k7y	136	75	influenza	influenza	NN
cord-001142-puj74k7y	136	76	vRNA	vRNA	JJS
cord-001142-puj74k7y	136	77	terminal	terminal	NN
cord-001142-puj74k7y	136	78	structure	structure	NN
cord-001142-puj74k7y	137	1	A	a	DT
cord-001142-puj74k7y	137	2	hairpin	hairpin	NN
cord-001142-puj74k7y	137	3	loop	loop	NN
cord-001142-puj74k7y	137	4	at	at	IN
cord-001142-puj74k7y	137	5	the	the	DT
cord-001142-puj74k7y	137	6	5	5	CD
cord-001142-puj74k7y	137	7	'	'	NN
cord-001142-puj74k7y	137	8	end	end	NN
cord-001142-puj74k7y	137	9	of	of	IN
cord-001142-puj74k7y	137	10	influenza	influenza	NN
cord-001142-puj74k7y	137	11	A	a	DT
cord-001142-puj74k7y	137	12	virus	virus	NN
cord-001142-puj74k7y	137	13	virion	virion	NN
cord-001142-puj74k7y	137	14	RNA	RNA	NNP
cord-001142-puj74k7y	137	15	is	be	VBZ
cord-001142-puj74k7y	137	16	required	require	VBN
cord-001142-puj74k7y	137	17	for	for	IN
cord-001142-puj74k7y	137	18	synthesis	synthesis	NN
cord-001142-puj74k7y	137	19	of	of	IN
cord-001142-puj74k7y	137	20	poly(A)+	poly(A)+	NNP
cord-001142-puj74k7y	138	1	mRNA	mRNA	NNP
cord-001142-puj74k7y	139	1	in	in	IN
cord-001142-puj74k7y	139	2	vitro	vitro	NNP
cord-001142-puj74k7y	139	3	Rescue	Rescue	NNP
cord-001142-puj74k7y	139	4	of	of	IN
cord-001142-puj74k7y	139	5	influenza	influenza	NN
cord-001142-puj74k7y	139	6	A	a	DT
cord-001142-puj74k7y	139	7	virus	virus	NN
cord-001142-puj74k7y	139	8	from	from	IN
cord-001142-puj74k7y	139	9	recombinant	recombinant	JJ
cord-001142-puj74k7y	139	10	Generation	generation	NN
cord-001142-puj74k7y	139	11	of	of	IN
cord-001142-puj74k7y	139	12	influenza	influenza	NN
cord-001142-puj74k7y	139	13	A	a	DT
cord-001142-puj74k7y	139	14	viruses	virus	NNS
cord-001142-puj74k7y	139	15	entirely	entirely	RB
cord-001142-puj74k7y	139	16	from	from	IN
cord-001142-puj74k7y	139	17	cloned	clone	VBN
cord-001142-puj74k7y	139	18	cDNAs	cDNAs	NNP
cord-001142-puj74k7y	139	19	Rescue	Rescue	NNP
cord-001142-puj74k7y	139	20	of	of	IN
cord-001142-puj74k7y	139	21	influenza	influenza	NN
cord-001142-puj74k7y	139	22	C	c	NN
cord-001142-puj74k7y	139	23	virus	virus	NN
cord-001142-puj74k7y	139	24	from	from	IN
cord-001142-puj74k7y	139	25	recombinant	recombinant	JJ
cord-001142-puj74k7y	139	26	A	a	DT
cord-001142-puj74k7y	139	27	molecular	molecular	JJ
cord-001142-puj74k7y	139	28	mechanism	mechanism	NN
cord-001142-puj74k7y	139	29	of	of	IN
cord-001142-puj74k7y	139	30	complement	complement	JJ
cord-001142-puj74k7y	139	31	resistance	resistance	NN
cord-001142-puj74k7y	139	32	of	of	IN
cord-001142-puj74k7y	139	33	human	human	JJ
cord-001142-puj74k7y	139	34	melanoma	melanoma	NN
cord-001142-puj74k7y	139	35	cells	cell	NNS
cord-001142-puj74k7y	140	1	Specific	specific	JJ
cord-001142-puj74k7y	140	2	residues	residue	NNS
cord-001142-puj74k7y	140	3	of	of	IN
cord-001142-puj74k7y	140	4	the	the	DT
cord-001142-puj74k7y	140	5	influenza	influenza	NN
cord-001142-puj74k7y	140	6	A	a	DT
cord-001142-puj74k7y	140	7	virus	virus	NN
cord-001142-puj74k7y	140	8	hemagglutinin	hemagglutinin	NN
cord-001142-puj74k7y	140	9	viral	viral	JJ
cord-001142-puj74k7y	140	10	RNA	RNA	NNP
cord-001142-puj74k7y	140	11	are	be	VBP
cord-001142-puj74k7y	140	12	important	important	JJ
cord-001142-puj74k7y	140	13	for	for	IN
cord-001142-puj74k7y	140	14	efficient	efficient	JJ
cord-001142-puj74k7y	140	15	packaging	packaging	NN
cord-001142-puj74k7y	140	16	into	into	IN
cord-001142-puj74k7y	140	17	budding	bud	VBG
cord-001142-puj74k7y	140	18	virions	virion	NNS
cord-001142-puj74k7y	140	19	Development	Development	NNP
cord-001142-puj74k7y	140	20	of	of	IN
cord-001142-puj74k7y	140	21	a	a	DT
cord-001142-puj74k7y	140	22	quantitative	quantitative	JJ
cord-001142-puj74k7y	140	23	assay	assay	NN
cord-001142-puj74k7y	140	24	for	for	IN
cord-001142-puj74k7y	140	25	SARS	SARS	NNP
cord-001142-puj74k7y	140	26	coronavirus	coronavirus	NN
cord-001142-puj74k7y	140	27	and	and	CC
cord-001142-puj74k7y	140	28	correlation	correlation	NN
cord-001142-puj74k7y	140	29	of	of	IN
cord-001142-puj74k7y	140	30	GAPDH	GAPDH	NNP
cord-001142-puj74k7y	140	31	mRNA	mrna	NN
cord-001142-puj74k7y	140	32	with	with	IN
cord-001142-puj74k7y	140	33	SARS	SARS	NNP
cord-001142-puj74k7y	140	34	coronavirus	coronavirus	NN
cord-001142-puj74k7y	140	35	in	in	IN
cord-001142-puj74k7y	140	36	clinical	clinical	JJ
cord-001142-puj74k7y	140	37	specimens	specimen	NNS
cord-001142-puj74k7y	140	38	Naked	Naked	NNP
cord-001142-puj74k7y	140	39	RNA	RNA	NNP
cord-001142-puj74k7y	140	40	immunization	immunization	NN
cord-001142-puj74k7y	140	41	with	with	IN
cord-001142-puj74k7y	140	42	replicons	replicon	NNS
cord-001142-puj74k7y	140	43	derived	derive	VBN
cord-001142-puj74k7y	140	44	from	from	IN
cord-001142-puj74k7y	140	45	poliovirus	poliovirus	NN
cord-001142-puj74k7y	140	46	and	and	CC
cord-001142-puj74k7y	140	47	Semliki	Semliki	NNP
cord-001142-puj74k7y	140	48	Forest	Forest	NNP
cord-001142-puj74k7y	140	49	virus	virus	NN
cord-001142-puj74k7y	140	50	genomes	genome	NNS
cord-001142-puj74k7y	140	51	for	for	IN
cord-001142-puj74k7y	140	52	the	the	DT
cord-001142-puj74k7y	140	53	generation	generation	NN
cord-001142-puj74k7y	140	54	of	of	IN
cord-001142-puj74k7y	140	55	a	a	DT
cord-001142-puj74k7y	140	56	cytotoxic	cytotoxic	JJ
cord-001142-puj74k7y	140	57	T	t	NN
cord-001142-puj74k7y	140	58	cell	cell	NN
cord-001142-puj74k7y	140	59	response	response	NN
cord-001142-puj74k7y	140	60	against	against	IN
cord-001142-puj74k7y	140	61	the	the	DT
cord-001142-puj74k7y	140	62	influenza	influenza	NN
cord-001142-puj74k7y	140	63	A	a	DT
cord-001142-puj74k7y	140	64	virus	virus	NN
cord-001142-puj74k7y	140	65	nucleoprotein	nucleoprotein	NNP
cord-001142-puj74k7y	140	66	Incorporation	Incorporation	NNP
cord-001142-puj74k7y	140	67	of	of	IN
cord-001142-puj74k7y	140	68	influenza	influenza	NN
cord-001142-puj74k7y	140	69	A	a	DT
cord-001142-puj74k7y	140	70	virus	virus	NN
cord-001142-puj74k7y	140	71	genome	genome	NN
cord-001142-puj74k7y	140	72	segments	segment	NNS
cord-001142-puj74k7y	140	73	does	do	VBZ
cord-001142-puj74k7y	140	74	not	not	RB
cord-001142-puj74k7y	140	75	absolutely	absolutely	RB
cord-001142-puj74k7y	140	76	require	require	VB
cord-001142-puj74k7y	140	77	wild	wild	JJ
cord-001142-puj74k7y	140	78	-	-	HYPH
cord-001142-puj74k7y	140	79	type	type	NN
cord-001142-puj74k7y	140	80	sequences	sequence	NNS
cord-001142-puj74k7y	141	1	The	the	DT
cord-001142-puj74k7y	141	2	multifunctional	multifunctional	JJ
cord-001142-puj74k7y	141	3	NS1	ns1	NN
cord-001142-puj74k7y	141	4	protein	protein	NN
cord-001142-puj74k7y	141	5	of	of	IN
cord-001142-puj74k7y	141	6	influenza	influenza	NN
cord-001142-puj74k7y	141	7	A	a	NN
cord-001142-puj74k7y	141	8	viruses	virus	NNS
cord-001142-puj74k7y	141	9	Direct	direct	JJ
cord-001142-puj74k7y	141	10	interaction	interaction	NN
cord-001142-puj74k7y	141	11	of	of	IN
cord-001142-puj74k7y	141	12	cellular	cellular	JJ
cord-001142-puj74k7y	141	13	hnRNP	hnrnp	NN
cord-001142-puj74k7y	141	14	-	-	HYPH
cord-001142-puj74k7y	141	15	F	f	NN
cord-001142-puj74k7y	141	16	and	and	CC
cord-001142-puj74k7y	141	17	NS1	ns1	NN
cord-001142-puj74k7y	141	18	of	of	IN
cord-001142-puj74k7y	141	19	influenza	influenza	NN
cord-001142-puj74k7y	141	20	A	a	DT
cord-001142-puj74k7y	141	21	virus	virus	NN
cord-001142-puj74k7y	141	22	accelerates	accelerate	VBZ
cord-001142-puj74k7y	141	23	viral	viral	JJ
cord-001142-puj74k7y	141	24	replication	replication	NN
cord-001142-puj74k7y	141	25	by	by	IN
cord-001142-puj74k7y	141	26	modulation	modulation	NN
cord-001142-puj74k7y	141	27	of	of	IN
cord-001142-puj74k7y	141	28	viral	viral	JJ
cord-001142-puj74k7y	141	29	transcriptional	transcriptional	JJ
cord-001142-puj74k7y	141	30	activity	activity	NN
cord-001142-puj74k7y	141	31	and	and	CC
cord-001142-puj74k7y	141	32	host	host	NN
cord-001142-puj74k7y	141	33	gene	gene	NN
cord-001142-puj74k7y	141	34	expression	expression	NN
cord-001142-puj74k7y	141	35	Regulation	regulation	NN
cord-001142-puj74k7y	141	36	of	of	IN
cord-001142-puj74k7y	141	37	influenza	influenza	NN
cord-001142-puj74k7y	141	38	virus	virus	NN
cord-001142-puj74k7y	141	39	RNA	rna	NN
cord-001142-puj74k7y	141	40	polymerase	polymerase	NN
cord-001142-puj74k7y	141	41	activity	activity	NN
cord-001142-puj74k7y	141	42	by	by	IN
cord-001142-puj74k7y	141	43	cellular	cellular	JJ
cord-001142-puj74k7y	141	44	and	and	CC
cord-001142-puj74k7y	141	45	viral	viral	JJ
cord-001142-puj74k7y	141	46	factors	factor	NNS
cord-001142-puj74k7y	142	1	NS2	NS2	NNP
cord-001142-puj74k7y	142	2	/	/	SYM
cord-001142-puj74k7y	142	3	NEP	NEP	NNP
cord-001142-puj74k7y	142	4	protein	protein	NN
cord-001142-puj74k7y	142	5	regulates	regulate	VBZ
cord-001142-puj74k7y	142	6	transcription	transcription	NN
cord-001142-puj74k7y	142	7	and	and	CC
cord-001142-puj74k7y	142	8	replication	replication	NN
cord-001142-puj74k7y	142	9	of	of	IN
cord-001142-puj74k7y	142	10	the	the	DT
cord-001142-puj74k7y	142	11	influenza	influenza	NN
cord-001142-puj74k7y	142	12	virus	virus	NN
cord-001142-puj74k7y	142	13	RNA	RNA	NNP
cord-001142-puj74k7y	142	14	genome	genome	NN
cord-001142-puj74k7y	143	1	Influenza	Influenza	NNP
cord-001142-puj74k7y	143	2	A	a	DT
cord-001142-puj74k7y	143	3	virus	virus	NN
cord-001142-puj74k7y	143	4	-	-	HYPH
cord-001142-puj74k7y	143	5	generated	generate	VBN
cord-001142-puj74k7y	143	6	small	small	JJ
cord-001142-puj74k7y	143	7	RNAs	rna	NNS
cord-001142-puj74k7y	143	8	regulate	regulate	VBP
cord-001142-puj74k7y	143	9	the	the	DT
cord-001142-puj74k7y	143	10	switch	switch	NN
cord-001142-puj74k7y	143	11	from	from	IN
cord-001142-puj74k7y	143	12	transcription	transcription	NN
cord-001142-puj74k7y	143	13	to	to	IN
cord-001142-puj74k7y	143	14	replication	replication	NN
cord-001142-puj74k7y	143	15	Activation	activation	NN
cord-001142-puj74k7y	143	16	of	of	IN
cord-001142-puj74k7y	143	17	influenza	influenza	NN
cord-001142-puj74k7y	143	18	virus	virus	NN
cord-001142-puj74k7y	143	19	RNA	RNA	NNP
cord-001142-puj74k7y	143	20	polymerase	polymerase	NN
cord-001142-puj74k7y	143	21	by	by	IN
cord-001142-puj74k7y	143	22	the	the	DT
cord-001142-puj74k7y	143	23	5	5	CD
cord-001142-puj74k7y	143	24	'	'	''
cord-001142-puj74k7y	143	25	and	and	CC
cord-001142-puj74k7y	143	26	3	3	CD
cord-001142-puj74k7y	143	27	'	'	POS
cord-001142-puj74k7y	143	28	terminal	terminal	JJ
cord-001142-puj74k7y	143	29	duplex	duplex	NN
cord-001142-puj74k7y	143	30	of	of	IN
cord-001142-puj74k7y	143	31	genomic	genomic	JJ
cord-001142-puj74k7y	143	32	RNA	RNA	NNP
cord-001142-puj74k7y	143	33	Influenza	Influenza	NNP
cord-001142-puj74k7y	143	34	virus	virus	NN
cord-001142-puj74k7y	143	35	pleiomorphy	pleiomorphy	NN
cord-001142-puj74k7y	143	36	characterized	characterize	VBN
cord-001142-puj74k7y	143	37	by	by	IN
cord-001142-puj74k7y	143	38	cryoelectron	cryoelectron	NNP
cord-001142-puj74k7y	143	39	tomography	tomography	NNP
cord-001142-puj74k7y	143	40	Free	free	JJ
cord-001142-puj74k7y	143	41	energy	energy	NN
cord-001142-puj74k7y	143	42	calculation	calculation	NN
cord-001142-puj74k7y	143	43	of	of	IN
cord-001142-puj74k7y	143	44	modified	modify	VBN
cord-001142-puj74k7y	143	45	base	base	NN
cord-001142-puj74k7y	143	46	-	-	HYPH
cord-001142-puj74k7y	143	47	pair	pair	NN
cord-001142-puj74k7y	143	48	formation	formation	NN
cord-001142-puj74k7y	143	49	in	in	IN
cord-001142-puj74k7y	143	50	explicit	explicit	JJ
cord-001142-puj74k7y	143	51	solvent	solvent	NN
cord-001142-puj74k7y	143	52	:	:	:
cord-001142-puj74k7y	143	53	A	a	DT
cord-001142-puj74k7y	143	54	predictive	predictive	JJ
cord-001142-puj74k7y	143	55	model	model	NN
cord-001142-puj74k7y	144	1	We	-PRON-	PRP
cord-001142-puj74k7y	144	2	are	be	VBP
cord-001142-puj74k7y	144	3	grateful	grateful	JJ
cord-001142-puj74k7y	144	4	to	to	IN
cord-001142-puj74k7y	144	5	G.	G.	NNP
cord-001142-puj74k7y	144	6	Brownlee	Brownlee	NNP
cord-001142-puj74k7y	144	7	for	for	IN
cord-001142-puj74k7y	144	8	providing	provide	VBG
cord-001142-puj74k7y	144	9	the	the	DT
cord-001142-puj74k7y	144	10	original	original	JJ
cord-001142-puj74k7y	144	11	plasmids	plasmid	NNS
cord-001142-puj74k7y	144	12	for	for	IN
cord-001142-puj74k7y	144	13	the	the	DT
cord-001142-puj74k7y	144	14	influenza	influenza	NN
cord-001142-puj74k7y	144	15	type	type	NN
cord-001142-puj74k7y	144	16	A	a	DT
cord-001142-puj74k7y	144	17	virus	virus	NN
cord-001142-puj74k7y	144	18	reverse	reverse	NN
cord-001142-puj74k7y	144	19	genetics	genetics	NN
cord-001142-puj74k7y	144	20	system	system	NN
cord-001142-puj74k7y	144	21	and	and	CC
cord-001142-puj74k7y	144	22	R.	R.	NNP
cord-001142-puj74k7y	144	23	Frade	Frade	NNP
cord-001142-puj74k7y	144	24	(	(	-LRB-
cord-001142-puj74k7y	144	25	Saint	Saint	NNP
cord-001142-puj74k7y	144	26	Antoine	Antoine	NNP
cord-001142-puj74k7y	144	27	Hospital	Hospital	NNP
cord-001142-puj74k7y	144	28	,	,	,
cord-001142-puj74k7y	144	29	Paris	Paris	NNP
cord-001142-puj74k7y	144	30	,	,	,
cord-001142-puj74k7y	144	31	France	France	NNP
cord-001142-puj74k7y	144	32	)	)	-RRB-
cord-001142-puj74k7y	144	33	for	for	IN
cord-001142-puj74k7y	144	34	the	the	DT
cord-001142-puj74k7y	144	35	SK	SK	NNP
cord-001142-puj74k7y	144	36	93/2	93/2	NN
cord-001142-puj74k7y	144	37	cells	cell	NNS
cord-001142-puj74k7y	144	38	.	.	.
cord-001142-puj74k7y	145	1	We	-PRON-	PRP
cord-001142-puj74k7y	145	2	thank	thank	VBP
cord-001142-puj74k7y	145	3	D.	D.	NNP
cord-001142-puj74k7y	145	4	Marc	Marc	NNP
cord-001142-puj74k7y	145	5	(	(	-LRB-
cord-001142-puj74k7y	145	6	INRA	INRA	NNP
cord-001142-puj74k7y	145	7	,	,	,
cord-001142-puj74k7y	145	8	Tours	Tours	NNP
cord-001142-puj74k7y	145	9	,	,	,
cord-001142-puj74k7y	145	10	France	France	NNP
cord-001142-puj74k7y	145	11	)	)	-RRB-
cord-001142-puj74k7y	145	12	for	for	IN
cord-001142-puj74k7y	145	13	anti	anti	JJ
cord-001142-puj74k7y	145	14	-	-	JJ
cord-001142-puj74k7y	145	15	NS1	ns1	JJ
cord-001142-puj74k7y	145	16	polyclonal	polyclonal	JJ
cord-001142-puj74k7y	145	17	antibodies	antibody	NNS
cord-001142-puj74k7y	145	18	,	,	,
cord-001142-puj74k7y	145	19	V.	V.	NNP
cord-001142-puj74k7y	145	20	Frigard	Frigard	NNP
cord-001142-puj74k7y	145	21	for	for	IN
cord-001142-puj74k7y	145	22	technical	technical	JJ
cord-001142-puj74k7y	145	23	assistance	assistance	NN
cord-001142-puj74k7y	145	24	,	,	,
cord-001142-puj74k7y	145	25	D.	D.	NNP
cord-001142-puj74k7y	145	26	Marc	Marc	NNP
cord-001142-puj74k7y	145	27	,	,	,
cord-001142-puj74k7y	145	28	G.	G.	NNP
cord-001142-puj74k7y	145	29	Abou	Abou	NNP
cord-001142-puj74k7y	145	30	-	-	HYPH
cord-001142-puj74k7y	145	31	Jaoudé	Jaoudé	NNP
cord-001142-puj74k7y	145	32	,	,	,
cord-001142-puj74k7y	145	33	D.	D.	NNP
cord-001142-puj74k7y	145	34	Moisy	Moisy	NNP
cord-001142-puj74k7y	145	35	and	and	CC
cord-001142-puj74k7y	145	36	S.	S.	NNP
cord-001142-puj74k7y	145	37	Munier	Munier	NNP
cord-001142-puj74k7y	145	38	for	for	IN
cord-001142-puj74k7y	145	39	helpful	helpful	JJ
cord-001142-puj74k7y	145	40	discussions	discussion	NNS
cord-001142-puj74k7y	145	41	and	and	CC
cord-001142-puj74k7y	145	42	critical	critical	JJ
cord-001142-puj74k7y	145	43	reading	reading	NN
cord-001142-puj74k7y	145	44	of	of	IN
cord-001142-puj74k7y	145	45	the	the	DT
cord-001142-puj74k7y	145	46	manuscript	manuscript	NN
cord-001142-puj74k7y	145	47	.	.	.
cord-001142-puj74k7y	146	1	Conceived	conceive	VBN
cord-001142-puj74k7y	146	2	and	and	CC
cord-001142-puj74k7y	146	3	designed	design	VBD
cord-001142-puj74k7y	146	4	the	the	DT
cord-001142-puj74k7y	146	5	experiments	experiment	NNS
cord-001142-puj74k7y	146	6	:	:	:
cord-001142-puj74k7y	146	7	BCC	BCC	NNP
cord-001142-puj74k7y	146	8	SW	SW	NNP
cord-001142-puj74k7y	146	9	.	.	.
cord-001142-puj74k7y	147	1	Performed	perform	VBN
cord-001142-puj74k7y	147	2	the	the	DT
cord-001142-puj74k7y	147	3	experiments	experiment	NNS
cord-001142-puj74k7y	147	4	:	:	:
cord-001142-puj74k7y	147	5	BCC	BCC	NNP
cord-001142-puj74k7y	147	6	.	.	.
cord-001142-puj74k7y	148	1	Analyzed	analyze	VBD
cord-001142-puj74k7y	148	2	the	the	DT
cord-001142-puj74k7y	148	3	data	datum	NNS
cord-001142-puj74k7y	148	4	:	:	:
cord-001142-puj74k7y	148	5	BCC	BCC	NNP
cord-001142-puj74k7y	148	6	CB	CB	NNP
cord-001142-puj74k7y	148	7	SW	SW	NNP
cord-001142-puj74k7y	148	8	.	.	.
cord-001142-puj74k7y	149	1	Contributed	contribute	VBN
cord-001142-puj74k7y	149	2	reagents	reagent	NNS
cord-001142-puj74k7y	149	3	/	/	SYM
cord-001142-puj74k7y	149	4	materials	material	NNS
cord-001142-puj74k7y	149	5	/	/	SYM
cord-001142-puj74k7y	149	6	analysis	analysis	NN
cord-001142-puj74k7y	149	7	tools	tool	NNS
cord-001142-puj74k7y	149	8	:	:	:
cord-001142-puj74k7y	149	9	BCC	BCC	NNP
cord-001142-puj74k7y	149	10	CB	CB	NNP
cord-001142-puj74k7y	149	11	.	.	.
cord-001142-puj74k7y	150	1	Wrote	write	VBD
cord-001142-puj74k7y	150	2	the	the	DT
cord-001142-puj74k7y	150	3	manuscript	manuscript	NN
cord-001142-puj74k7y	150	4	:	:	:
cord-001142-puj74k7y	150	5	BCC	BCC	NNP
cord-001142-puj74k7y	150	6	CB	CB	NNP
cord-001142-puj74k7y	150	7	SW	SW	NNP
cord-001142-puj74k7y	150	8	.	.	.