id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-355259-779czzzx	Yang, Xiaoyun	A Beneficiary Role for Neuraminidase in Influenza Virus Penetration through the Respiratory Mucus	2014-10-15		.txt	text/plain	6143	324	47	Swine influenza virus (SIV) has a strong tropism for pig respiratory mucosa, which consists of a mucus layer, epithelium, basement membrane and lamina propria. The microscopic diffusion of SIV particles in the mucus was analyzed by single particle tracking (SPT), and the macroscopic penetration of SIV through mucus was studied by a virus in-capsule-mucus penetration system, followed by visualizing the translocation of the virions with time by immunofluorescence staining. These findings clearly show that the neuraminidase helps SIV move through the mucus, which is important for the virus to reach and infect epithelial cells and eventually become shed into the lumen of the respiratory tract. To this purpose, we applied swine influenza virus to a model we previously set up using porcine respiratory mucus, pseudorabies virus (PRV) and single particle tracking (SPT) [20] . This does not only confirm the beneficial effect of neuraminidase on releasing SIV from respiratory mucus, but also highlights bidirectional synergistic interactions between influenza virus and bacterial infections.	./cache/cord-355259-779czzzx.txt	./txt/cord-355259-779czzzx.txt