id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-354052-x4ckzw64	Li, Chunhua	Manipulation of the Porcine Epidemic Diarrhea Virus Genome Using Targeted RNA Recombination	2013-08-02		.txt	text/plain	4932	256	53	We have established a reverse genetics system for PEDV based on targeted RNA recombination that allows the modification of the 3′-end of the viral genome, which encodes the structural proteins and the ORF3 protein. All mPEDV infected cells stained positive both with the polyclonal MHV serum and with the monoclonal antibody directed against the PEDV nucleocapsid protein confirming the purity and the identity of the chimeric virus. Donor RNAs transcribed from the pPEDV and pPEDV-DORF3 transfer vectors were electroporated into mPEDV-infected L cells after which we were able to recover and purify the r-wtPEDV and PEDV-DORF3 viruses in VERO cells. Thus, the PEDV-DORF3/GFP and PEDV-DORF3/RLuc virus were preincubated with dilutions of serum obtained from an experimentally PEDV-infected pig and control serum, and the mixtures were subsequently added to VERO cells and incubated after which the GFP and Renilla luciferase expression was recorded at 9 and 6 hours p.i., respectively (Fig. 5D) .	./cache/cord-354052-x4ckzw64.txt	./txt/cord-354052-x4ckzw64.txt