id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-353253-kk2q71vg	Itokawa, Kentaro	Disentangling primer interactions improves SARS-CoV-2 genome sequencing by multiplex tiling PCR	2020-09-18		.txt	text/plain	3327	179	52	Soon after the pandemic was recognized by epidemiologists, a group of biologists comprising the ARTIC Network, has devised a multiplexed polymerase chain reaction (PCR) protocol and primer set for targeted whole-genome amplification of SARS-CoV-2. In our experience, the low to zero depth for those two amplicons was the most frequent bottleneck for using the ARTIC primer set V1 to sequence all targeted genomic regions from samples with middle to low viral load (Ct > 27). The results indicated that preventing primer dimerformation is an effective measure to improve coverage bias in the ARTIC Network's SARS-CoV-2 genome sequencing protocol, and may be applicable to other PrimalSeq methods in general. The formation of primer-dimers is a major cause of coverage bias in the ARTIC Network's multiplex PCR protocol for SARS-CoV-2 genome sequencing. A proposal of an alternative primer for the ARTIC Network's multiplex PCR to improve coverage of SARS-CoV-2 genome sequencing (manuscript version 1)	./cache/cord-353253-kk2q71vg.txt	./txt/cord-353253-kk2q71vg.txt