id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-352564-2j4pjjwk	Dominguez, Samuel R.	Human Coronavirus HKU1 Infection of Primary Human Type II Alveolar Epithelial Cells: Cytopathic Effects and Innate Immune Response	2013-07-24		.txt	text/plain	4449	199	46	Because they are the natural target cells for respiratory virus infection, primary human respiratory epithelial cell cultures provide the ideal in vitro systems for investigation of cell factors required for growth of respiratory human viruses, for analysis of their interactions with viruses and their innate immune responses to infection, and for isolation and propagation of novel respiratory pathogens. Here we demonstrate that HCoV-HKU1 can infect and be serially propagated in primary human alveolar type II cells but not in alveolar type I-like cells or alveolar macrophages at the air-liquid interface. Titers of viral RNA in the wash from the apical surface of the cells were determined by qRT-PCR at the indicated time points, and HCoV-HKU1 Infection of Type II Alveolar Cells PLOS ONE | www.plosone.org cultures were fixed and immunolabeled with antibodies to the HCoV-HKU1 spike glycoprotein to identify infected cells. In summary, HCoV-HKU1 can infect, be serially propagated, and induce an anti-viral response in human alveolar type II cells maintained at an air-liquid interface.	./cache/cord-352564-2j4pjjwk.txt	./txt/cord-352564-2j4pjjwk.txt