id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-351868-w4d45fue	Zuwała, Kaja	The Nucleocapsid Protein of Human Coronavirus NL63	2015-02-20		.txt	text/plain	6708	421	53	Surprisingly, analysis of the subcellular localization of the N protein of HCoV-NL63 revealed that, differently than homologous proteins from other coronaviral species except for SARS-CoV, it is not present in the nucleus of infected or transfected cells. In order to test subcellular localization of the N protein in LLC-MK2 cells, the maxFP-Green-N/NL63-N encoding RNA was prepared based on the original plasmid. For EMSA assay 10 μg of RNA or DNA corresponding in sequence to the N-NL63 gene (prepared in the same manner as for the transfection of eukaryotic cells) was incubated in buffered solution (5 mM Tris, 50 mM NaCl, pH8.0) with 10 μg of the NTD or CTD for 30 minutes at room temperature. The constructs of NTD and CTD used in this study were designed based on literature data, HCoV-NL63 N protein amino acid sequence alignment with known homologs and on the comparative analysis of currently available crystal structures of these homologs.	./cache/cord-351868-w4d45fue.txt	./txt/cord-351868-w4d45fue.txt