id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-350398-w75flrwv	Sampath, Rangarajan	Comprehensive Biothreat Cluster Identification by PCR/Electrospray-Ionization Mass Spectrometry	2012-06-29		.txt	text/plain	11138	581	50	Coupling biothreat cluster-specific PCR to electrospray ionization mass spectrometry simultaneously provides the breadth of coverage, discrimination of near neighbors, and an extremely low false positive rate due to the requirement that an amplicon with a precise base composition of a biothreat agent be detected by mass spectrometry. In addition to detecting the threat organisms, the biothreat assay described here also detects virulence factors associated with three of the agents: Bacillus anthracis (pXO1 and pXO2), Yersinia pestis (pla and caf), and Vibrio cholera (ctx1). PCR primers were designed to conserved regions within the selected target genes such that the targeted threat agent was clearly identified and differentiated from its near-neighbor species ( Table 1) . In the biothreat assay, the Francisella biocluster is identified by two genus-specific primer pairs targeting the asd (BCT2328) and galE (BCT2332) genes ( Table 1) .	./cache/cord-350398-w75flrwv.txt	./txt/cord-350398-w75flrwv.txt