id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-348243-e5tdb08v	Schermer, Bernhard	Rapid SARS-CoV-2 testing in primary material based on a novel multiplex RT-LAMP assay	2020-11-02		.txt	text/plain	3958	236	56	METHODS: To avoid these obstacles, we tested PCR-independent methods for the detection of SARS-CoV-2 RNA from primary material (nasopharyngeal swabs) including reverse transcription loop-mediated isothermal amplification (RT-LAMP) and specific high-sensitivity enzymatic reporter unlocking (SHERLOCK). To allow for the comparison of different nucleic acid detection methods for SARS-CoV-2 we collected redundant material from nasopharyngeal swabs obtained for qPCR testing in clinical routine due to suspected COVID-19. We first tested two recently described assays for SARS-CoV-2 detection on isolated RNA from patient samples. In summary, our multiplex RT-LAMP protocol is a simple and sensitive way to detect SARS-CoV-2 RNA from clinical samples. Currently, a test based on our multiplexed RT-LAMP assay would-in contrast to a good specificity-most likely miss to identify those infected patients with very low amounts of viral RNA in the nose or throat and would not yet reach the sensitivity of the gold-standard qPCR assays.	./cache/cord-348243-e5tdb08v.txt	./txt/cord-348243-e5tdb08v.txt