id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-320938-f526k9q1	Chen, Hongjun	Partial and Full PCR-Based Reverse Genetics Strategy for Influenza Viruses	2012-09-28		.txt	text/plain	8614	455	60	In order to determine whether a Flu PCR amplicon could be transfected into cells and be amplified by the influenza polymerase complex, a PCR product was produced encoding the GFP reporter gene in negative orientation flanked by the influenza segment 7 untranslated regions (UTRs) and further flanked by the human pol1 promoter and the mouse t1 termination signal, pol1EGFPt1 (Fig. 1A, Fig. S1A , Table S1 ). doi:10.1371/journal.pone.0046378.g001 Efficient influenza virus rescue using Flu PCR amplicons in either ''1+7'' or ''2+6'' modes The pol1HA pdm t1 or pol1HA D072 t1 HA PCR amplicons (Table 1) were co-transfected into co-cultured 293T/MDCK cells in a ''1+7'' mode along with 7 RG plasmids encoding the corresponding additional gene segments from the influenza A/Puerto Rico/ 8/1934 (H1N1) strain (PR8).	./cache/cord-320938-f526k9q1.txt	./txt/cord-320938-f526k9q1.txt