id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-318614-518giv0m	Tsai, Jih-Jin	A fully automated sample-to-answer PCR system for easy and sensitive detection of dengue virus in human serum and mosquitos	2019-07-10		.txt	text/plain	4825	213	49	A pan-dengue virus (DENV) RT-iiPCR, targeting the 5' untranslated region, was validated previously on the semi-automated POCKIT combo system (involving separate devices for nucleic acid extraction and PCR amplification/detection) to offer performance comparable to a laboratory real-time PCR. CONCLUSIONS/SIGNIFICANCE: With performance comparable to a previously validated system, the fully-automated PCR system allows applications of the pan-DENV reagent as a useful tool near points of need to facilitate easy, fast and effective detection of dengue virus and help mitigate versatile public health challenges in the control and management of dengue disease. Testing with the pan-DENV RT-iiPCR, the analytical and clinical performance of the fully automatic POCKIT Central system was comparable to those of the semi-automatic POCKI combo system, which was validated previously to offer performances equivalent to the CDC DENV1-4 real-time RT-PCR for the detection of DENV in human serum [7, 24, 26] .	./cache/cord-318614-518giv0m.txt	./txt/cord-318614-518giv0m.txt