id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-309471-lr68epyb	Xia, Jingya	Virus-Specific Immune Memory at Peripheral Sites of Herpes Simplex Virus Type 2 (HSV-2) Infection in Guinea Pigs	2014-12-08		.txt	text/plain	7010	312	42	Memory B cells were detected primarily in the spleen and to a lesser extent in bone marrow but not in the genital tract or neural tissues suggesting that the HSV-specific antibody-secreting cells present at peripheral sites of HSV-2 infection represented persisting populations of plasma cells. The presence of memory B cells in the culture is therefore detected as a TLR-agonist-induced increase in the number of antigen-specific, ASCs. To test for the presence of memory B cells at peripheral tissues, we stimulated lymphocyte populations isolated from bone marrow, spleen, vagina/cervix, or spinal cord/sensory ganglia of previously infected guinea pigs with a combination of LPS and CpG oligonucleotides and quantified HSV-specific ASC by ELISPOT. On day 7 post infection, HSV-specific, IFN-c-secreting T cells from the spleen, vagina/cervix, and lumbosacral ganglia and the adjacent spinal cord were quantified using a pair Peripheral Tissue-Resident Immune Memory to HSV-2 of previously described anti-guinea pig IFN-c -specific monoclonal antibodies [27] in an IFN-c ELISPOT assay.	./cache/cord-309471-lr68epyb.txt	./txt/cord-309471-lr68epyb.txt