id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-309043-dlmx12vt	von Brunn, Albrecht	Analysis of Intraviral Protein-Protein Interactions of the SARS Coronavirus ORFeome	2007-05-23		.txt	text/plain	6706	341	52	The severe acute respiratory syndrome coronavirus (SARS-CoV) genome is predicted to encode 14 functional open reading frames, leading to the expression of up to 30 structural and non-structural protein products. There are reports that a number of MHV and SARS-CoV replicase proteins colocalize and eventually interact in cytoplasmic membrane bound complexes, in which viral RNA synthesis occurs [18, 19] . We therefore cloned the SARS-CoV ORFeome by recombinatorial cloning (GATEWAY technology) and performed a genome-wide analysis for viral protein interactions by yeast-two-hybrid (Y2H) matrix screen. To systematically study the subcellular localization of viral proteins within eukaryotic HeLa cells the SARS-CoV ORFs were transfected in eukaryotic vectors with either N-or C-terminal Flag tags and detected with an anti-Flag antibody. In this study we report the cloning of the complete ORFeome of SARS-CoV and the results of a matrix-based yeast two-hybrid screen of pairwise viral protein-protein interactions.	./cache/cord-309043-dlmx12vt.txt	./txt/cord-309043-dlmx12vt.txt