id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-308764-9z4qcoqz	Wei, Lin	Transcriptome Analysis of Houttuynia cordata Thunb. by Illumina Paired-End RNA Sequencing and SSR Marker Discovery	2014-01-02		.txt	text/plain	5253	298	51	cordata to generate an enormous transcriptome sequence dataset for gene discovery and molecular marker development. cordata; 43 (86%) produced fragments of expected size, suggesting that the unigenes were suitable for specific primer design and of high quality, and the SSR marker could be widely used in marker-assisted selection and molecular breeding of H. cordata and used Illumina paired-end sequencing technology to generate a large-scale EST database and develop a set of SSR markers. For further assessment of the assembly quality and development of new molecular markers, all 63,954 unigenes generated in this study were used to mine potential microsatellites, which were defined as di-to hexa-nucleotide SSRs with a minimum of four repetitions for all motifs. Here, the N50 length of the unigenes was 1,051 bp and the average length was 679 bp, which suggests that the relatively short reads from Illumina paired-end sequencing for this non-model organism have been effectively and accurately assembled.	./cache/cord-308764-9z4qcoqz.txt	./txt/cord-308764-9z4qcoqz.txt