id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-306135-pt4jsr6d	Chan, Kamfai	A Rapid and Low-Cost PCR Thermal Cycler for Infectious Disease Diagnostics	2016-02-12		.txt	text/plain	6292	284	56	Many molecular diagnostic assays are developed based on using thermal cyclers to carry out polymerase chain reaction (PCR) and reverse-transcription PCR for DNA and RNA amplification and detection, respectively. This thermos thermal cycler (TTC) uses a very simple design that performs PCR amplification based on the "archaic" method of hand-transferring reaction tubes through a series of water baths, minimizing the temperature ramping time needed for PCR tubes to reach thermal equilibrium (Fig 1) . The TTC RT-PCR was performed using protocols similar to the HIV test, with PCR tubes transferred between three thermoses (reverse transcription, denaturation, and annealing/extension) and an optional room-temperature water bath. The gel photo in Fig 3 shows that the TTC can produce multiplexed amplicons with the correct sizes and that the yield is similar to a three-step reaction performed in the commercial cycler with same number of PCR cycles.	./cache/cord-306135-pt4jsr6d.txt	./txt/cord-306135-pt4jsr6d.txt