id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-305811-987dhnf7	Cho, Che-Pei	Regulation of Programmed Ribosomal Frameshifting by Co-Translational Refolding RNA Hairpins	2013-04-29		.txt	text/plain	5837	301	49	Because both 59CC-WT and 13363-13520 constructs share 27 identical nucleotides upstream of their slippery sites, the attenuation activity difference is not likely to be caused by an E-site flanking sequences effect [12, 13] but rather by the disruption of the two potential AU base pairs. We noticed a potential to form four extra base pairs between 59and 39-flanking sequences (GACG and CGUU, respectively) of the 6BPGC hairpin stem (and other deletion mutants) due to the existence of a 59 SalI cloning site (Fig. S1A ). The results (Fig. S1C ) indicate that the two potential base pairs involving E-site sequences are not the main cause of observed attenuation activity in 293T cell cultures. Furthermore, mutating two nucleotides (27 nucleotides upstream of the E site) to disrupt Watson-Crick base pairs in the lower hairpin stem dramatically impairs attenuation activity (Fig. 2) , indicating that attenuation is not caused by primary sequencemediated flanking-sequences effects [12, 13] .	./cache/cord-305811-987dhnf7.txt	./txt/cord-305811-987dhnf7.txt