id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-305303-82n96ukr	Shapira, Assaf	Removal of Hepatitis C Virus-Infected Cells by a Zymogenized Bacterial Toxin	2012-02-16		.txt	text/plain	10132	431	42	As shown in Figure 2 , similar numbers of surviving colonies were observed when the cells were transfected with the plasmids encoding mCherry-NS3 activated MazF or the red fluorescent protein alone, suggesting that expression of NS3-activable ribonuclease in naïve HEK293 T-REx cells (that do not express NS3) cause minimal toxicity, if any. The ER membrane-targeted zymoxin colocalizes with NS3 protease in vivo Previously we described a HEK293 cell line which inducibly expresses (by addition of tetracycline) a fusion between EGFP and the coding sequence of the full length NS3 (including the helicase domain) followed by NS4A from HCV 1a genotype [10] . When infection reached ,50% (about 50% of the cultured cells showed expression of the HCV-core protein, as detected by immuno-staining and fluorescence microscopy), the mixed culture and a culture of uninfected cells were treated with NS3 activated MazF or uncleavable-MazF encoding adenoviruses at MOI of ,3.	./cache/cord-305303-82n96ukr.txt	./txt/cord-305303-82n96ukr.txt