id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-302962-qw6s1t7j	Hause, Ben M.	Bovine Rhinitis Viruses Are Common in U.S. Cattle with Bovine Respiratory Disease	2015-03-19		.txt	text/plain	4948	280	56	Metagenomic sequencing of a nasal swab from a bovine respiratory disease (BRD) diagnostic submission from Kansas identified contigs with approximately 90% nucleotide similarity to BRAV2 and BRBV. Altogether, these results demonstrate that BRV infections are common in cattle with respiratory disease and that BRAV1, BRAV2 and BRBV co-circulate in U.S. cattle and have high similarity to viruses isolated more than 30 years ago from diverse locations. The PCR assay specificity was confirmed using bovine rhinitis virus positive samples as determined by metagenomic sequencing as well as with cultures of common BRDC pathogens BVDV, BHV-1, BRSV, BCV, Mannheimia haemolytica, Histophilus somni, Pasteurella multocida and Mycoplasma bovis. As metagenomic sequencing of a nasal swab from a calf with acute respiratory disease identified concurrent infection with two bovine rhinitis virus species, we designed a 5'-nuclease reverse transcription PCR (rtPCR) assay targeting the conserved 3D polymerase gene to investigate the incidence of BRV in BRDC diagnostic submissions.	./cache/cord-302962-qw6s1t7j.txt	./txt/cord-302962-qw6s1t7j.txt