id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-301180-ndiwmnv0	Lin, Min-Hsuan	Nullbasic, a Potent Anti-HIV Tat Mutant, Induces CRM1-Dependent Disruption of HIV Rev Trafficking	2012-12-10		.txt	text/plain	5161	233	39	Importantly, treatment of Myc-Rev and Nullbasic-mCherry expressing cells with LMB resulted in the complete restoration of Myc-Rev nucleolar accumulation (Fig. 3, row 6) , suggesting that Nullbasic requires functional CRM1 to mislocalize Rev most likely by an indirect interaction. While expression of Nullbasic-mCherry alone had no apparent effect on the nucleolar localization of either protein (Fig. 6 , rows 2 and 5), coexpression of Nullbasic-mCherry with Myc-Rev induced relocalization of C23 but not fibrillarin from the nucleolus to the nucleoplasm, in a manner concomitant with the redistribution of Myc-Rev (Fig. 6, rows 3 and 6 , respectively). CRM1 is a cellular receptor that recognizes and binds to the leucine-rich NES domain of Rev, an interaction which facilitates the egress of Rev/viral mRNA complexes through nuclear pores [3, 4, 42] and is readily visualized in cells as a colocalization in the nucleolus (Fig. 3, row 2 ).	./cache/cord-301180-ndiwmnv0.txt	./txt/cord-301180-ndiwmnv0.txt