id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-298078-uqrwq5qk	Kwak, Hoyun	Annexin A2 Binds RNA and Reduces the Frameshifting Efficiency of Infectious Bronchitis Virus	2011-08-30		.txt	text/plain	5346	318	56	The results suggest that ANXA2 is a cellular RBP that can modulate the frameshifting efficiency of viral RNA, enabling it to act as an anti-viral cellular protein, and hinting at roles in RNA metabolism for other cellular mRNAs. Ribosomal frameshifing is a recoding process of translation where a specific messenger RNA (mRNA)-mediated signal directs a ribosome to shift its reading frame and to continue in the new frame. To search for cellular proteins that directly interacted with IBV pseudoknot RNA, a RNA pull down assay was performed in the presence of cell extracts (Figure 2A ). Through the RNA-immunoprecipitation assay, we showed that ANXA2 specifically interacted with wild-type IBV pseudoknot RNA but not with mutant IBV RNA in LNCaP and HEK293T cells ( Figure 3C and 3D). To test how ANXA2 regulates the frameshifting efficiency of IBV pseudoknot RNA, we first overexpressed ANXA2 protein in the presence of the reporters and measured the luciferase activities.	./cache/cord-298078-uqrwq5qk.txt	./txt/cord-298078-uqrwq5qk.txt