id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-293393-kbndie8e	Braesch-Andersen, Sten	ApoD Mediates Binding of HDL to LDL and to Growing T24 Carcinoma	2014-12-16		.txt	text/plain	6253	386	60	To investigate the presence of particles containing both apoD and apoB, we performed a dual-specific ELISA using the anti-apoD mAb D554 as a capture antibody and a biotinylated anti-apoB mAb, LDL20, for detection. To determine the importance of having intact lipoprotein particles, we repeated the dual-specific apoD/B ELISA in the presence of detergent, using anti-apoD-D544 as the capture mAb and anti-apoB-LDL20 as the detecting mAb. As seen in Fig. 4A , only small amounts of apoB bound to D544 when detergent was present, indicating that intact lipoprotein particles are needed for the apoD-LDL interaction. In agreement with this latter finding, apoD production was high in confluent, growth-arrested T24 cell cultures (2,5 million cells in 9 ml), but was not detectable in non-confluent proliferating Anti-apoD (D544) was used as the capture antibody and anti-apoB (LDL20-biotin) was used as the detection antibody in a detergent free dualspecific ELISA.	./cache/cord-293393-kbndie8e.txt	./txt/cord-293393-kbndie8e.txt